Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Reverse (-)

3592846 .. 3613681

Gene Structure 20836 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1042000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5448 bp

ATGCACCTCTACAATGCATGGCTGCCGCCTCCGGTTGCCGAGGAGTCGAAGAAGGAGAAGGAGTCATTCTCTTGGGTCGTCTCCTCCGTCAAGAACTCGTACAAACCAGACGATCCTGAATCAGTCTATGCCACTCTCAAATGGGTTTCTGTTATCGACCTTTTCGTCAAGGCGAAAAGTGAAGTATCCTTGGAAGATGTAACTGTTCTTGTTGAATTTGGATTGGAATTGTTTTATGTATCGCAAAATAAGCTTTATGCCCAGGTTAGATGGGGAAACATCCTAGTCAAACTACTCAATAAGCATCGAAAGAAGTTGTCTTTGAAATTAAAATGGCGGCCTTTGTACGAAACATTGATCCATACACATTTCACTAGGAGTACAGGTCCAGAAGGCTGGAGATTGAGACAACGACATTTTGAGGCTACAACTTCTCTTGTTCGGTCTTGCCGGAAGTTCTTCCCACCAGGTTCTGCCTTTGAGATATGGTCCGAGTTTAGATCTTTATTGGAAAATCCCTGGCATAATTCATCCTTCGAGGGTTCCGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGACTTCTTTTCACATGATTGGATTAAAGAGTTTTTCCACCTCTGGGACTCGATACCCAATTGCCAATTTTGGAACAGTCAATGGGCAGCTGTTATTGCTCGTGTTGTAAAGAACTACAAGTTGATTGATTGGGAGTGTTACTTGCCCGCACTTTTCACCAGATACTTAAATATGTTTGAGGTTCCTGTGGCAAATGGAAGTGGATCATATCCATTTTCTGTGGATGTTCCAAGATACACAAGATTCTTGTTCTCCAATAAGACAGTTACCCCAGCAAAGGCCATCGCAAAGTCCATTGTCTATTTGCTAAGGCCAGGTAGCTCAGCACAGGAGCATTTTGAAAAATTGGTCAACCTCTTAGAACAATATTACCATCCTTCTAATGGTGGTCGCTGGACTTATGCATTAGAACGCTTTTTGCTCTATTTGGTTATTTCATTCCGGAAACGCTTACAGCATGAACAACTGAGCATTGATAAAGATAGACATGATGAACAGTACCTAGGAAGATCAGAAAGGAATTTCTTTGTCAGGGTGGTGCTCAAGTTAATTGATCGTGGTCAATATAGCAAGAATGAACATCTTTCTGAAACAGTTGCTGCTGCAACTTCGATTTTGTCTTATGTGGAGCCTACTCTGGTCCTTCCTTTTGTCGCATCTCGCTTCCAAATGGCCTTGGAGACGATGACTGCCACCCACCAGTTGCAGATTGCAGTGACGTCGGTAGCATTTGTTGGGCGTTCATTGTTCCTCACGTCCCTATCAGATTCTGCAGTAAAATCAGTTGATCCTGGTAATAGTGATGAATTCATTGAGCTTTTGATGGTTTCACTATCCAATGCATTACTTGGTATGGATGCCAATGATCCTCCAAAAACCTTGGCAACAATGCAATTAATTGGCTCTATATTTTCCAATATGTCTTCATTGGATGATAATATTCATGAGCTATCTGTCATGCCAATGATTCGGTTTTCTGAATGGTTAGATGAGTTCTTGTGTCGCCTATTTTCCTTACTCTTACACCTGGAACCAAGCAGTGTTACGAATGAAGGCCTACATTCATCGGCAGCGTCAGGAACTTTTCTAGTTGAGGATGGTCCTTACTACTATTGCATGCTTGAAATCTTGCTCGGGAGACTTTCACAACCTCTCTATAATCAGGCTTTGAAGAAGATCTCCAAGTTTGTGAAGACAAATATTCTTCCAGGGGCAATTGCAGAGGTTGGACTGCTTTGTTGCGCATGTGTTCATTCCAACCCAGAAGAAGCAGTTACTCAACTTGTCAAGCCAATTTTATTGTCTGTTATTTCTTCTTTGGAAGGAACACCAGCTACAGGATTTGGAGGTAGAGGAATATGTGGCGCTTCAGTTTCAACTAAGGTTAAGCCAACAATTTCTCCAGCTCTTGAAACAGCAATCGACTATCAATTAAAAGTATTATCAGTTGCCATAAGTTATGGAGGTCCTGTGCTTCTGCGCTACAAGGATGACTTCAAAGAGGCGATACTTTCTGCTTTTGAGTCCCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGATCTCTCCTTGGAAGCCTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTGTTTTGCATCATCCAAATGCTGCTGCATTAGAGGAATGGATCAGCATCAAAGATTATTCTAATGATAAACCATTGGCGGTCCCCAAGTGGCATATCCCAAGTGTTGAAGAAGTTGAATTTGCAAATGAACTTTTGGACCTTCATTTTCGGATGGCTTTAGATGATCTTTCAAGAATATGTGAAACTAAAATCCACTCAGATCCAGGAGATGAGAAAGAGCACTTGAAAGTGACTCTTCTGCGTATAGATTCTTCATTGCAAGGTGTTTTGTCTTGCTTGCCTGACTTCGCCCCATCCTCAAGGAATGGGACTGTTGAACATCCAAATCATGCTGCTTTTCTAATAGCCGGAGCAACAGGTTCAACTGTTGGTACCACTAAATTGCGGGAAAAAGCTGCTGACATTATACATGCAGCCTGCAAATACATACTAGAGAAGAAATCAGATGATAGCATCCTGTTGATACTCATTATTCGAATTATGGATGCTTTAGGAAACTATGGAAGTTTGGAGTATGATGAGTGGTCAAATCACCGGCAGGCATGGAAACTAGAATCTGCTGCCATTGTAGAACCTTCAGTAAACTTTATAAATTATGCTCATTCGAAGGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCATTTATGCACAGTACATGGAGATCATCACAATCATCTTATCATATATACCGTACTAATGGGAATTTTGGTCCACCAGACCATGTGAATCTTTTGGTGGATAATCTTCTAAATCTCTCATTGCATAGCTACGAAACTGTTCGTTTACTTGCTGGGAAATCTCTTTTAAAGATGATAAAGAGATGGCCATCTATGATTTCAAAGTGCGTCCTTTCTCTAACAGAGAACTTACGGAACCCCAAGTCACCAGAATATGCAGTGCTAGGATCATGTGCAGTCCTTGCCACACAGACAGTTCTCAAGCATTTGACAATGGACCCAAAGGCGTTCTCTTCGTGTATTCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAATCCCAGAAAGCAATCAATGAGCTTTTTGTCAAATACAACATTCACTTTGCTGGAGTATCTAGAAGCATTTTCAGGGCATCAGGCAATAATACAGAAGCACCAGATTTTGCAGATTTGGTTTCTCAGATCACTTCTATGAGTTTTGATTCTACTGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTCTACTCTTGTTGGCTATGTCATCTAGGAATGACCCAACTTCCTCCTCAAAAATTTTGAGTGAAACTGCCGGTCACTTCTTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTTGCAATTTCCGCACTAAATACACTGTTAAAAGAATCACCTTATAAACTGTCTGCTGGGGAACAGACTCTCCCCTCTGGGAATGTGCAAGGAAGTAGAAAGTCATCCCTTGAAGGAGAATTAACCCAAATATTTCAGGAAGATGGTTTTTTCAATGAGACCTTGAACAGTCTTTCTCATGTTCATATTGTTACTGATACTGAAAGCGCATCTTCCAGAGGCAATCATGGAAGTTCTTTTCAGAGCCTCGCAGACAAATCAATCACCCGATTTTATTTTGATTTTACCGCTTCATGGCCACGCACTCCTACTTGGATCTCTTTATTAGGGAGTGACACATTTTACTCAAACTTTGCGAGAATTTTCAAGCGGCTAATACAGGAATGTGGCATGCCTGTTCTATTGGCACTTAAAAGCTCATTAGAGGAGTTTGCAAATGCCAAGGAGAGGTCTAAGCAGTGTGTTGCTGCTGAAGCATTGGCTGGAATATTGCATTCTGATGTTGACGGTATTTTAGTAGCATGGGATACTTGGATTTTGACCCAGTTGCAGAATATTATCTTATCACAATCAGTAGAGTCAATACCTGAGTGGGCAGCCTGTATACGTTACGCTGTTACTGGGAAAGGGAAGCATGGAACAAGAGTTCCTCTTTTGAGGCAACCAGTATTGGATTGTTTGGCCACACCTTTACCTGGAACTGTAACCACTACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCAGCTGCACTTATAGAATTATCTCCACACACAATGCCATTAACTGAAGTACAGCTCCATCATAGACTTCTGGAAGAGCTGCTTGGTAGCATGTGCCATTCATCTGCCCAAGTAAGAGAAGCTTTAGGTGTTACCCTTTCAGTGGTGTGCTCTAACATCCAGCTTTATGAGTCATTTAATCATGAGCATTCACTTGAGGGGGGAAAAAGAGATGTTGCCAACAAATTTGATGGGAAAAGTTGGGTTCAGTTTCTGAAAGAGCGAGCATCTGAAGTGCTGATGAGTATTCAGAATACTTCCCAGTCTGACAGTTTGGATACCACAACAAATATTAGTTCTGAGAATGGGAATTCAAATGGTGATTCACAGGATGCTGTCAAGTGGATGGAGACGCTTTTCCATTTTATCATCTCATCTTTGAAGTCTGGCCGAGTTTCATATCTTCTGGATGTAATTGTGGGACTTCTTTCTCCTGTTATATCCTTACAGGAAACTTCAAGTAAAGATCTGTCAACCTTAGCGAAAGCAGCTTTTGAATTGCTGAAGTGGAGAATTTTCTGGGGACCTCATCTCCAGGAGGCTGTATCTGTGATTCTTTCTTCTGCCAATGACTCTAACTGGCGAATTAGATCTGCAACACTTACATATCTGCGAACGTTTATGTATAGGCACACCTTCATCCTCTCAAGTGCGGAGAAGCAGCAAATTTGGAGAACTGTTGAAAATCTACTTTTAGACAACCAAGTTGAGGTGAGAGAGCATGCTGCTGCAGTTTTAGCAGGCCTAATGAAGGGTGGGGATGAAGATCTTGCTAAAGACTTTCGCGATAAAGCTTATACCGAAGCTACTACTCTTCAGAGGAAGAGAAAGCGCAGAACATTGAGCTCAAGCCAGTCCATAGCTTCTATTCACGGTGCTGTACTTGCTTTGGTGGCTTCCGTGTTGTCAGCCCCTTATGATATGCCCAGTTGGTTGCCGGAGCATGTCACTTTACTTGCTCGTTTTAGTGGAGAGCCATCGCCGGTGAAATCTACGGTTACAAAAGCAGTTGCAGAGTTTCGACGTACCCATGCTGATACATGGAATATTCAGAAAGATTCATTTACTGAAGAGCAACTTGAGGTTCTGGCTGATACGTCGTCTTCATCGTCGTATTTCGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1816

Amino Acids

203.41

Weight (kDa)

7.05

Isoelectric Point (pI)

43.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	310 - 504	9.6e-28	Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid	PF16507	510 - 825	2.5e-19	Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C	PF11919	1730 - 1815	3.8e-28	Proteasome activator complex subunit 4-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2798, 3588
AasI	GACNNNNNNGTC	1 cut(s)	164
AatII	GACGTC	1 cut(s)	1310
Acc16I	TGCGCA	1 cut(s)	1831
Acc65I	GGTACC	1 cut(s)	2579
AccB1I	GGYRCC	1 cut(s)	2579
AccI	GTMKAC	1 cut(s)	4147
AccII	CGCG	2 cut(s)	2833, 5109
AccIII	TCCGGA	1 cut(s)	1029
AclI	AACGTT	1 cut(s)	4940
AclWI	GGATC	9 cut(s)	107, 352, 799, 1370, 1448, 2255, 2402, 3127, 3866
AcoI	YGGCCR	4 cut(s)	3038, 3839, 4224, 4711
AcuI	CTGAAG	8 cut(s)	1941, 2769, 4035, 4350, 4575, 4847, 5123, 5412
AcyI	GRCGYC	1 cut(s)	1307
AfeI	AGCGCT	1 cut(s)	4271
AjiI	CACGTC	1 cut(s)	1344
AjuI	GAANNNNNNNTTGG	2 cut(s)	4350, 4382
AloI	GAACNNNNNNTCC	8 cut(s)	2160, 2192, 3597, 3629, 4173, 4174, 4205, 4206
Alw21I	GWGCWC	4 cut(s)	1131, 2430, 4436, 5171
Alw26I	GTCTC	6 cut(s)	85, 400, 1262, 1720, 3695, 4667
AlwI	GGATC	9 cut(s)	107, 352, 799, 1370, 1448, 2255, 2402, 3127, 3866
AlwNI	CAGNNNCTG	4 cut(s)	473, 1187, 1358, 4537
Ama87I	CYCGRG	1 cut(s)	1721
Aor13HI	TCCGGA	1 cut(s)	1029
Aor51HI	AGCGCT	1 cut(s)	4271
AseI	ATTAAT	1 cut(s)	1484
Asp700I	GAANNNNTTC	3 cut(s)	458, 2991, 3205
Asp718I	GGTACC	1 cut(s)	2579
AspA2I	CCTAGG	1 cut(s)	1090
AspLEI	GCGC	6 cut(s)	1832, 1955, 2070, 3752, 4272, 5157
AsuC2I	CCSGG	1 cut(s)	547
AsuHPI	GGTGA	9 cut(s)	736, 2153, 2732, 3090, 3573, 3799, 4655, 5047, 5320
AsuII	TTCGAA	2 cut(s)	2683, 2813
AvaI	CYCGRG	1 cut(s)	1721
AvrII	CCTAGG	1 cut(s)	1090
BaeGI	GKGCMC	1 cut(s)	2842
BalI	TGGCCA	3 cut(s)	3040, 3841, 4226
BanI	GGYRCC	1 cut(s)	2579
BanII	GRGCYC	1 cut(s)	5171
BarI	GAAGNNNNNNTAC	2 cut(s)	2443, 2475
BauI	CACGAG	1 cut(s)	687
BbsI	GAAGAC	3 cut(s)	1503, 1787, 5418
Bbv12I	GWGCWC	4 cut(s)	1131, 2430, 4436, 5171
BcgI	CGANNNNNNTGC	2 cut(s)	1290, 1324
BciVI	GTATCC	4 cut(s)	196, 3534, 4063, 4594
BclI	TGATCA	2 cut(s)	2143, 2194
BcnI	CCSGG	1 cut(s)	547
BcoDI	GTCTC	6 cut(s)	85, 400, 1262, 1720, 3695, 4667
BfaI	CTAG	9 cut(s)	284, 375, 1091, 1676, 2639, 2759, 3116, 3294, 3450
BfmI	CTRYAG	4 cut(s)	1359, 1923, 4257, 5052
BfoI	RGCGCY	2 cut(s)	1956, 4273
BfuI	GTATCC	4 cut(s)	196, 3534, 4063, 4594
BglII	AGATCT	5 cut(s)	500, 1764, 4789, 4913, 5089
BlnI	CCTAGG	1 cut(s)	1090
BlpI	GCTNAGC	1 cut(s)	910
BmeT110I	CYCGRG	1 cut(s)	1721
BmgBI	CACGTC	1 cut(s)	1344
BmiI	GGNNCC	9 cut(s)	544, 771, 1218, 1620, 2289, 2581, 3089, 3171, 4848
BmrI	ACTGGG	4 cut(s)	4081, 4173, 4579, 5244
BmuI	ACTGGG	4 cut(s)	4081, 4173, 4579, 5244
BpiI	GAAGAC	3 cut(s)	1503, 1787, 5418
BplI	GAGNNNNNCTC	2 cut(s)	53, 85
BpmI	CTGGAG	4 cut(s)	418, 1974, 3306, 4841
Bpu10I	CCTNAGC	2 cut(s)	896, 4801
Bpu1102I	GCTNAGC	1 cut(s)	910
Bpu14I	TTCGAA	2 cut(s)	2683, 2813
BpuEI	CTTGAG	7 cut(s)	1115, 2491, 3137, 4499, 4954, 5155, 5423
BpuMI	CCSGG	1 cut(s)	547
BsaBI	GATNNNNATC	3 cut(s)	2252, 3040, 5088
BsaHI	GRCGYC	1 cut(s)	1307
BsaI	GGTCTC	1 cut(s)	3695
BsaWI	WCCGGW	2 cut(s)	31, 1029
Bse118I	RCCGGY	3 cut(s)	2742, 3494, 5305
Bse1I	ACTGG	9 cut(s)	1288, 3391, 4087, 4168, 4208, 4585, 4907, 5176, 5250
Bse3DI	GCAATG	2 cut(s)	2462, 2972
Bse8I	GATNNNNATC	3 cut(s)	2252, 3040, 5088
BseAI	TCCGGA	1 cut(s)	1029
BseJI	GATNNNNATC	3 cut(s)	2252, 3040, 5088
BseMI	GCAATG	2 cut(s)	2462, 2972
BseMII	CTCAG	7 cut(s)	924, 1046, 2418, 3371, 3545, 4122, 4614
BseNI	ACTGG	9 cut(s)	1288, 3391, 4087, 4168, 4208, 4585, 4907, 5176, 5250
BseRI	GAGGAG	4 cut(s)	56, 73, 3460, 3983
BseSI	GKGCMC	1 cut(s)	2842
BseYI	CCCAGC	3 cut(s)	859, 3005, 3599
BsgI	GTGCAG	2 cut(s)	3147, 4275
Bsh1236I	CGCG	2 cut(s)	2833, 5109
BshNI	GGYRCC	1 cut(s)	2579
BsiHKAI	GWGCWC	4 cut(s)	1131, 2430, 4436, 5171
BsiHKCI	CYCGRG	1 cut(s)	1721
BsiSI	CCGG	9 cut(s)	32, 451, 546, 1030, 2556, 2743, 3495, 5261, 5306
BslFI	GGGAC	7 cut(s)	647, 1330, 2098, 2273, 2530, 4758, 4860
BsmAI	GTCTC	6 cut(s)	85, 400, 1262, 1720, 3695, 4667
BsmBI	CGTCTC	3 cut(s)	85, 1262, 4667
BsmFI	GGGAC	7 cut(s)	647, 1330, 2098, 2273, 2530, 4758, 4860
BsmI	GAATGC	2 cut(s)	4036, 4471
Bso31I	GGTCTC	1 cut(s)	3695
BsoBI	CYCGRG	1 cut(s)	1721
Bsp119I	TTCGAA	2 cut(s)	2683, 2813
Bsp1286I	GDGCHC	5 cut(s)	1131, 2430, 2842, 4436, 5171
Bsp13I	TCCGGA	1 cut(s)	1029
Bsp1720I	GCTNAGC	1 cut(s)	910
Bsp68I	TCGCGA	1 cut(s)	5109
BspCNI	CTCAG	7 cut(s)	923, 1047, 2417, 3370, 3544, 4123, 4615
BspEI	TCCGGA	1 cut(s)	1029
BspFNI	CGCG	2 cut(s)	2833, 5109
BspHI	TCATGA	3 cut(s)	1531, 3220, 4465
BspLI	GGNNCC	9 cut(s)	544, 771, 1218, 1620, 2289, 2581, 3089, 3171, 4848
BspMAI	CTGCAG	2 cut(s)	1363, 5056
BspPI	GGATC	9 cut(s)	107, 352, 799, 1370, 1448, 2255, 2402, 3127, 3866
BspQI	GCTCTTC	2 cut(s)	4353, 5388
BspT104I	TTCGAA	2 cut(s)	2683, 2813
BspT107I	GGYRCC	1 cut(s)	2579
BspTNI	GGTCTC	1 cut(s)	3695
BsrDI	GCAATG	2 cut(s)	2462, 2972
BsrFI	RCCGGY	3 cut(s)	2742, 3494, 5305
BsrI	ACTGG	9 cut(s)	1288, 3391, 4087, 4168, 4208, 4585, 4907, 5176, 5250
BssAI	RCCGGY	3 cut(s)	2742, 3494, 5305
BssNAI	GTATAC	1 cut(s)	4148
BssNI	GRCGYC	1 cut(s)	1307
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	6 cut(s)	189, 1090, 1263, 1467, 2164, 3984
Bst1107I	GTATAC	1 cut(s)	4148
Bst2BI	CACGAG	1 cut(s)	687
Bst6I	CTCTTC	7 cut(s)	2448, 2816, 3190, 4353, 5141, 5142, 5388
BstACI	GRCGYC	1 cut(s)	1307
BstAPI	GCANNNNNTGC	1 cut(s)	3134
BstBI	TTCGAA	2 cut(s)	2683, 2813
BstDSI	CCRYGG	1 cut(s)	2831
BstENI	CCTNNNNNAGG	1 cut(s)	5072
BstFNI	CGCG	2 cut(s)	2833, 5109
BstH2I	RGCGCY	2 cut(s)	1956, 4273
BstHHI	GCGC	6 cut(s)	1832, 1955, 2070, 3752, 4272, 5157
BstMAI	GTCTC	6 cut(s)	85, 400, 1262, 1720, 3695, 4667
BstMWI	GCNNNNNNNGC	9 cut(s)	250, 683, 3134, 3554, 4016, 4811, 5060, 5207, 5216
BstNSI	RCATGY	7 cut(s)	1708, 1836, 2621, 3937, 4378, 5048, 5270
BstSFI	CTRYAG	4 cut(s)	1359, 1923, 4257, 5052
BstSLI	GKGCMC	1 cut(s)	2842
BstUI	CGCG	2 cut(s)	2833, 5109
BstV2I	GAAGAC	3 cut(s)	1503, 1787, 5418
BstX2I	RGATCY	7 cut(s)	500, 1764, 2407, 3858, 4789, 4913, 5089
BstXI	CCANNNNNNTGG	1 cut(s)	396
BstYI	RGATCY	7 cut(s)	500, 1764, 2407, 3858, 4789, 4913, 5089
BstZ17I	GTATAC	1 cut(s)	4148
BsuI	GTATCC	4 cut(s)	196, 3534, 4063, 4594
BtgI	CCRYGG	1 cut(s)	2831
BtgZI	GCGATG	2 cut(s)	856, 5286
BtrI	CACGTC	1 cut(s)	1344
BtsI	GCAGTG	4 cut(s)	1308, 1633, 3117, 4007
BtsIMutI	CAGTG	7 cut(s)	1308, 1633, 3117, 3227, 3566, 4007, 4431
BtuMI	TCGCGA	1 cut(s)	5109
CaiI	CAGNNNCTG	4 cut(s)	473, 1187, 1358, 4537
CciI	TCATGA	3 cut(s)	1531, 3220, 4465
CfoI	GCGC	6 cut(s)	1832, 1955, 2070, 3752, 4272, 5157
Cfr10I	RCCGGY	3 cut(s)	2742, 3494, 5305
Cfr42I	CCGCGG	1 cut(s)	2834
CseI	GACGC	3 cut(s)	1650, 3049, 4684
CsiI	ACCWGGT	1 cut(s)	466
DraI	TTTAAA	2 cut(s)	3021, 3516
DrdI	GACNNNNNNGTC	1 cut(s)	164
DseDI	GACNNNNNNGTC	1 cut(s)	164
EaeI	YGGCCR	4 cut(s)	3038, 3839, 4224, 4711
Eam1104I	CTCTTC	7 cut(s)	2448, 2816, 3190, 4353, 5141, 5142, 5388
EarI	CTCTTC	7 cut(s)	2448, 2816, 3190, 4353, 5141, 5142, 5388
Ecl136II	GAGCTC	1 cut(s)	5169
Eco130I	CCWWGG	6 cut(s)	189, 1090, 1263, 1467, 2164, 3984
Eco147I	AGGCCT	2 cut(s)	1644, 5067
Eco24I	GRGCYC	1 cut(s)	5171
Eco31I	GGTCTC	1 cut(s)	3695
Eco47III	AGCGCT	1 cut(s)	4271
Eco53kI	GAGCTC	1 cut(s)	5169
Eco57I	CTGAAG	8 cut(s)	1941, 2769, 4035, 4350, 4575, 4847, 5123, 5412
Eco88I	CYCGRG	1 cut(s)	1721
EcoICRI	GAGCTC	1 cut(s)	5169
EcoNI	CCTNNNNNAGG	1 cut(s)	5072
EcoO109I	RGGNCCY	2 cut(s)	2054, 4847
EcoRI	GAATTC	3 cut(s)	1394, 3201, 4633
EcoT14I	CCWWGG	6 cut(s)	189, 1090, 1263, 1467, 2164, 3984
EcoT22I	ATGCAT	3 cut(s)	19, 994, 1432
EcoT38I	GRGCYC	1 cut(s)	5171
ErhI	CCWWGG	6 cut(s)	189, 1090, 1263, 1467, 2164, 3984
Esp3I	CGTCTC	3 cut(s)	85, 1262, 4667
FaqI	GGGAC	7 cut(s)	647, 1330, 2098, 2273, 2530, 4758, 4860
FauI	CCCGC	2 cut(s)	742, 2586
FbaI	TGATCA	2 cut(s)	2143, 2194
FblI	GTMKAC	1 cut(s)	4147
FriOI	GRGCYC	1 cut(s)	5171
FspBI	CTAG	9 cut(s)	284, 375, 1091, 1676, 2639, 2759, 3116, 3294, 3450
FspI	TGCGCA	1 cut(s)	1831
GlaI	GCGC	6 cut(s)	1831, 1954, 2069, 3751, 4271, 5156
GsaI	CCCAGC	3 cut(s)	863, 3009, 3603
GsuI	CTGGAG	4 cut(s)	418, 1974, 3306, 4841
HaeII	RGCGCY	2 cut(s)	1956, 4273
HapII	CCGG	9 cut(s)	32, 451, 546, 1030, 2556, 2743, 3495, 5261, 5306
HgaI	GACGC	3 cut(s)	1650, 3049, 4684
HhaI	GCGC	6 cut(s)	1832, 1955, 2070, 3752, 4272, 5157
Hin1I	GRCGYC	1 cut(s)	1307
Hin6I	GCGC	6 cut(s)	1830, 1953, 2068, 3750, 4270, 5155
HinP1I	GCGC	6 cut(s)	1830, 1953, 2068, 3750, 4270, 5155
HincII	GTYRAC	3 cut(s)	940, 4048, 4797
HindII	GTYRAC	3 cut(s)	940, 4048, 4797
HindIII	AAGCTT	3 cut(s)	251, 4404, 5115
HpaII	CCGG	9 cut(s)	32, 451, 546, 1030, 2556, 2743, 3495, 5261, 5306
HphI	GGTGA	9 cut(s)	736, 2153, 2732, 3090, 3573, 3799, 4655, 5047, 5320
Hpy166II	GTNNAC	7 cut(s)	940, 2791, 2927, 2999, 4048, 4148, 4797
Hpy8I	GTNNAC	7 cut(s)	940, 2791, 2927, 2999, 4048, 4148, 4797
Hpy99I	CGWCG	4 cut(s)	1312, 5349, 5425, 5437
HpyCH4IV	ACGT	6 cut(s)	1307, 1343, 4150, 4940, 5347, 5420
HpyF10VI	GCNNNNNNNGC	9 cut(s)	250, 683, 3134, 3554, 4016, 4811, 5060, 5207, 5216
HpySE526I	ACGT	6 cut(s)	1307, 1343, 4150, 4940, 5347, 5420
Hsp92I	GRCGYC	1 cut(s)	1307
HspAI	GCGC	6 cut(s)	1830, 1953, 2068, 3750, 4270, 5155
Kpn2I	TCCGGA	1 cut(s)	1029
KpnI	GGTACC	1 cut(s)	2583
Ksp22I	TGATCA	2 cut(s)	2143, 2194
KspI	CCGCGG	1 cut(s)	2834
LguI	GCTCTTC	2 cut(s)	4353, 5388
LmnI	GCTCC	6 cut(s)	919, 1216, 2135, 2558, 4344, 5263
MabI	ACCWGGT	1 cut(s)	466
MaeI	CTAG	9 cut(s)	284, 375, 1091, 1676, 2639, 2759, 3116, 3294, 3450
MaeII	ACGT	6 cut(s)	1307, 1343, 4150, 4940, 5347, 5420
MfeI	CAATTG	2 cut(s)	646, 1803
MflI	RGATCY	7 cut(s)	500, 1764, 2407, 3858, 4789, 4913, 5089
MhlI	GDGCHC	5 cut(s)	1131, 2430, 2842, 4436, 5171
MlsI	TGGCCA	3 cut(s)	3040, 3841, 4226
MluNI	TGGCCA	3 cut(s)	3040, 3841, 4226
MlyI	GAGTC	9 cut(s)	53, 71, 629, 2120, 2434, 3604, 4130, 4463, 4889
MmeI	TCCRAC	2 cut(s)	1795, 1869
Mox20I	TGGCCA	3 cut(s)	3040, 3841, 4226
Mph1103I	ATGCAT	3 cut(s)	19, 994, 1432
MroI	TCCGGA	1 cut(s)	1029
MroXI	GAANNNNTTC	3 cut(s)	458, 2991, 3205
MscI	TGGCCA	3 cut(s)	3040, 3841, 4226
MslI	CAYNNNNRTG	4 cut(s)	1550, 2223, 3368, 4041
Msp20I	TGGCCA	3 cut(s)	3040, 3841, 4226
MspA1I	CMGCKG	3 cut(s)	677, 2833, 4289
MspI	CCGG	9 cut(s)	32, 451, 546, 1030, 2556, 2743, 3495, 5261, 5306
MunI	CAATTG	2 cut(s)	646, 1803
Mva1269I	GAATGC	2 cut(s)	4036, 4471
MvnI	CGCG	2 cut(s)	2833, 5109
MwoI	GCNNNNNNNGC	9 cut(s)	250, 683, 3134, 3554, 4016, 4811, 5060, 5207, 5216
NciI	CCSGG	1 cut(s)	547
NlaIV	GGNNCC	9 cut(s)	544, 771, 1218, 1620, 2289, 2581, 3089, 3171, 4848
NmeAIII	GCCGAG	2 cut(s)	64, 4739
NmuCI	GTSAC	6 cut(s)	1303, 2437, 3096, 3497, 3875, 5269
NruI	TCGCGA	1 cut(s)	5109
NsbI	TGCGCA	1 cut(s)	1831
NsiI	ATGCAT	3 cut(s)	19, 994, 1432
NspI	RCATGY	7 cut(s)	1708, 1836, 2621, 3937, 4378, 5048, 5270
NspV	TTCGAA	2 cut(s)	2683, 2813
PaeI	GCATGC	3 cut(s)	1708, 3937, 5048
PagI	TCATGA	3 cut(s)	1531, 3220, 4465
PceI	AGGCCT	2 cut(s)	1644, 5067
PciSI	GCTCTTC	2 cut(s)	4353, 5388
PcsI	WCGNNNNNNNCGW	3 cut(s)	84, 162, 5429
PctI	GAATGC	2 cut(s)	4036, 4471
PdmI	GAANNNNTTC	3 cut(s)	458, 2991, 3205
PfoI	TCCNGGA	2 cut(s)	2410, 4857
PleI	GAGTC	9 cut(s)	52, 70, 629, 2119, 2434, 3604, 4129, 4462, 4889
PpsI	GAGTC	9 cut(s)	52, 70, 629, 2119, 2434, 3604, 4129, 4462, 4889
PpuMI	RGGWCCY	2 cut(s)	2054, 4847
PshBI	ATTAAT	1 cut(s)	1484
PsiI	TTATAA	2 cut(s)	2798, 3588
Psp124BI	GAGCTC	1 cut(s)	5171
Psp1406I	AACGTT	1 cut(s)	4940
Psp5II	RGGWCCY	2 cut(s)	2054, 4847
PspFI	CCCAGC	3 cut(s)	859, 3005, 3599
PspN4I	GGNNCC	9 cut(s)	544, 771, 1218, 1620, 2289, 2581, 3089, 3171, 4848
PspPPI	RGGWCCY	2 cut(s)	2054, 4847
PsrI	GAACNNNNNNTAC	2 cut(s)	1906, 1938
PstI	CTGCAG	2 cut(s)	1363, 5056
PstNI	CAGNNNCTG	4 cut(s)	473, 1187, 1358, 4537
PsuI	RGATCY	7 cut(s)	500, 1764, 2407, 3858, 4789, 4913, 5089
PvuII	CAGCTG	2 cut(s)	677, 4289
RruI	TCGCGA	1 cut(s)	5109
RseI	CAYNNNNRTG	4 cut(s)	1550, 2223, 3368, 4041
SacI	GAGCTC	1 cut(s)	5171
SacII	CCGCGG	1 cut(s)	2834
SapI	GCTCTTC	2 cut(s)	4353, 5388
SchI	GAGTC	9 cut(s)	53, 71, 629, 2120, 2434, 3604, 4130, 4463, 4889
SduI	GDGCHC	5 cut(s)	1131, 2430, 2842, 4436, 5171
SexAI	ACCWGGT	1 cut(s)	466
SfcI	CTRYAG	4 cut(s)	1359, 1923, 4257, 5052
Sfr303I	CCGCGG	1 cut(s)	2834
SfuI	TTCGAA	2 cut(s)	2683, 2813
SgrAI	CRCCGGYG	1 cut(s)	5305
SgrBI	CCGCGG	1 cut(s)	2834
SmiMI	CAYNNNNRTG	4 cut(s)	1550, 2223, 3368, 4041
SmlI	CTYRAG	7 cut(s)	1130, 2506, 3152, 4478, 4969, 5170, 5402
SmoI	CTYRAG	7 cut(s)	1130, 2506, 3152, 4478, 4969, 5170, 5402
SphI	GCATGC	3 cut(s)	1708, 3937, 5048
SseBI	AGGCCT	2 cut(s)	1644, 5067
SspI	AATATT	8 cut(s)	956, 1528, 1789, 3675, 4032, 4099, 4615, 5371
SspMI	CTAG	9 cut(s)	284, 375, 1091, 1676, 2639, 2759, 3116, 3294, 3450
SstI	GAGCTC	1 cut(s)	5171
StuI	AGGCCT	2 cut(s)	1644, 5067
StyI	CCWWGG	6 cut(s)	189, 1090, 1263, 1467, 2164, 3984
TaiI	ACGT	6 cut(s)	1310, 1346, 4153, 4943, 5350, 5423
TaqII	GACCGA	1 cut(s)	432
TatI	WGTACW	5 cut(s)	380, 2199, 2867, 4333, 5203
TauI	GCSGC	4 cut(s)	28, 340, 2833, 3916
TscAI	CASTG	7 cut(s)	1308, 1633, 3117, 3234, 3573, 4007, 4431
TseFI	GTSAC	6 cut(s)	1303, 2437, 3096, 3497, 3875, 5269
Tsp45I	GTSAC	6 cut(s)	1303, 2437, 3096, 3497, 3875, 5269
TspGWI	ACGGA	3 cut(s)	76, 3100, 5212
TspRI	CASTG	7 cut(s)	1308, 1633, 3117, 3234, 3573, 4007, 4431
VspI	ATTAAT	1 cut(s)	1484
XagI	CCTNNNNNAGG	1 cut(s)	5072
XbaI	TCTAGA	1 cut(s)	3293
XceI	RCATGY	7 cut(s)	1708, 1836, 2621, 3937, 4378, 5048, 5270
XcmI	CCANNNNNNNNNTGG	1 cut(s)	968
XmaJI	CCTAGG	1 cut(s)	1090
XmiI	GTMKAC	1 cut(s)	4147
XmnI	GAANNNNTTC	3 cut(s)	458, 2991, 3205
XspI	CTAG	9 cut(s)	284, 375, 1091, 1676, 2639, 2759, 3116, 3294, 3450
ZraI	GACGTC	1 cut(s)	1308
Zsp2I	ATGCAT	3 cut(s)	19, 994, 1432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.