MD01G1012100.v1.1

Belongs to the bacterial ribosomal protein bL17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008477
Physical Location & Seq
Forward (+)
6318537 .. 6322098
3562 bp
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UTR
Exon/CDS
Intron
MD01G1012100.v1.1.491

Sequence Viewer

Length: 438 bp
ATGACGAAGTTCAGGAAGCTAAACCGACCCACGGGTCACCGCATGTCCATGCTCAGGACGATGGTGTCGCAGTTGGTGAAGCACGAGCGCATTGAGACCACTGTTTCCAAGGCCAAAGAGATCCGTCGCGAGGCTGATAAAATGGTGCAGCTTGGAAAAGATGGCTCCTTGTGTGCCGCAAGGCGTGCTGCTGCTTTTGTGCGTGGGGATAATGTACTTCACAAGCTATTTACTGAAATGGCTTATCGATACAAAGATCGAGTAGGTGGATACACAAGGCTACTTCGCACTCGTATACGAGTTGGTGATGCTGCACCTATGGCCTATATTGAGTTCATCGACAGAGAAAATGAGCTCAGACAATCAAAACCACCAGCCCCCCAACCACCTCAGAGAGTTCCTTTGGATCCTTGGGCGAGATCCAACCTTTGCAAGTAG

Protein Analysis

146

Amino Acids

16.8

Weight (kDa)

10.64

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L17 PF01196 17 - 113 1.4e-34 Ribosomal protein L17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 33, 64
AccI GTMKAC 1 cut(s) 295
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 40, 177
AclWI GGATC 4 cut(s) 115, 401, 414, 414
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 3 cut(s) 31, 54, 130
AluBI AGCT 4 cut(s) 19, 151, 226, 355
AluI AGCT 4 cut(s) 19, 151, 226, 355
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 115, 401, 414, 414
AoxI GGCC 2 cut(s) 111, 321
ApeKI GCWGC 4 cut(s) 148, 188, 191, 311
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 3 cut(s) 29, 88, 317
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 357
BauI CACGAG 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 4 cut(s) 160, 175, 178, 298
BccI CCATC 2 cut(s) 55, 155
BciVI GTATCC 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 5 cut(s) 149, 177, 189, 192, 312
BlsI GCNGC 5 cut(s) 150, 178, 190, 193, 313
BmiI GGNNCC 2 cut(s) 166, 408
BmsI GCATC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 30, 108, 410
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 54, 130
BseCI ATCGAT 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 30, 108, 410
BseLI CCNNNNNNNGG 3 cut(s) 31, 54, 130
BseMII CTCAG 3 cut(s) 67, 370, 404
BseXI GCAGC 4 cut(s) 160, 175, 178, 298
BsgI GTGCAG 2 cut(s) 167, 297
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 113, 323
BshVI ATCGAT 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 31, 54, 130
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 2 cut(s) 113, 323
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 4 cut(s) 120, 256, 406, 419
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 2 cut(s) 40, 177
BspANI GGCC 2 cut(s) 113, 323
BspCNI CTCAG 3 cut(s) 66, 369, 403
BspDI ATCGAT 1 cut(s) 247
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 166, 408
BspPI GGATC 4 cut(s) 115, 401, 414, 414
BspTNI GGTCTC 1 cut(s) 89
BssECI CCNNGG 3 cut(s) 30, 108, 410
BssMI GATC 4 cut(s) 120, 256, 406, 419
BssNAI GTATAC 1 cut(s) 296
BssSI CACGAG 1 cut(s) 83
BssT1I CCWWGG 2 cut(s) 108, 410
Bst1107I GTATAC 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 53, 356, 390
BstDSI CCRYGG 1 cut(s) 30
BstEII GGTNACC 1 cut(s) 35
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 4 cut(s) 123, 259, 409, 422
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 120, 256, 406, 419
BstMWI GCNNNNNNNGC 2 cut(s) 185, 320
BstNSI RCATGY 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 35
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 160, 175, 178, 298
BstX2I RGATCY 3 cut(s) 120, 406, 419
BstYI RGATCY 3 cut(s) 120, 406, 419
BstZ17I GTATAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 247
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 113, 323
BsuTUI ATCGAT 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 99
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 247
Csp6I GTAC 1 cut(s) 215
CviAII CATG 2 cut(s) 43, 49
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 3 cut(s) 53, 356, 390
DpnI GATC 4 cut(s) 122, 258, 408, 421
DpnII GATC 4 cut(s) 120, 256, 406, 419
DrdI GACNNNNNNGTC 2 cut(s) 33, 64
DseDI GACNNNNNNGTC 2 cut(s) 33, 64
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 2 cut(s) 108, 410
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 355
Eco91I GGTNACC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 355
EcoO65I GGTNACC 1 cut(s) 35
EcoT14I CCWWGG 2 cut(s) 108, 410
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 2 cut(s) 108, 410
FaeI CATG 2 cut(s) 46, 52
FaiI YATR 5 cut(s) 44, 50, 296, 320, 327
FatI CATG 2 cut(s) 42, 48
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 149, 177, 189, 192, 312
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 5 cut(s) 149, 177, 189, 192, 312
GlaI GCGC 1 cut(s) 89
GluI GCNGC 5 cut(s) 149, 177, 189, 192, 312
HaeIII GGCC 2 cut(s) 113, 323
HhaI GCGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 46, 52
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HphI GGTGA 3 cut(s) 29, 88, 317
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 2 cut(s) 359, 393
Hpy188III TCNNGA 3 cut(s) 13, 55, 128
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4V TGCA 3 cut(s) 148, 314, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 320
HpyF3I CTNAG 3 cut(s) 53, 356, 390
Hsp92II CATG 2 cut(s) 46, 52
HspAI GCGC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 120, 256, 406, 419
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 2 cut(s) 40, 387
Lsp1109I GCAGC 4 cut(s) 160, 175, 178, 298
LweI GCATC 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 35
MalI GATC 4 cut(s) 122, 258, 408, 421
MboI GATC 4 cut(s) 120, 256, 406, 419
MflI RGATCY 3 cut(s) 120, 406, 419
MhlI GDGCHC 1 cut(s) 357
MnlI CCTC 2 cut(s) 124, 399
MslI CAYNNNNRTG 1 cut(s) 47
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 185, 320
NdeII GATC 4 cut(s) 120, 256, 406, 419
NlaIII CATG 2 cut(s) 46, 52
NlaIV GGNNCC 2 cut(s) 166, 408
NmuCI GTSAC 1 cut(s) 35
NruI TCGCGA 1 cut(s) 129
NspI RCATGY 1 cut(s) 46
PkrI GCNGC 5 cut(s) 150, 178, 190, 193, 313
Psp124BI GAGCTC 1 cut(s) 357
PspEI GGTNACC 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 166, 408
PsuI RGATCY 3 cut(s) 120, 406, 419
RruI TCGCGA 1 cut(s) 129
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 357
SatI GCNGC 5 cut(s) 149, 177, 189, 192, 312
Sau3AI GATC 4 cut(s) 120, 256, 406, 419
SduI GDGCHC 1 cut(s) 357
SetI ASST 8 cut(s) 21, 153, 228, 268, 319, 357, 391, 429
SfaNI GCATC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 47
SsiI CCGC 2 cut(s) 40, 177
SstI GAGCTC 1 cut(s) 357
StyI CCWWGG 2 cut(s) 108, 410
TaaI ACNGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 247, 259, 339
TatI WGTACW 1 cut(s) 214
TauI GCSGC 1 cut(s) 179
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 4 cut(s) 148, 188, 191, 311
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 325
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 106
XceI RCATGY 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.