MD01G1016700.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005875
Physical Location & Seq
Reverse (-)
7584474 .. 7597025
12552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1016700.v1.1.491

Sequence Viewer

Length: 798 bp
ATGCAAATACCCAGATGGAGAAACGTCTTGCTCCTCAAGAATTCATTGGTTTCATCAACTTCTGCAGCTACCCGTTTCGCTTCATTCCATTCTACTCCTGCTTCGTGTGAGAAGTGGAAGAACAAATGGACCTTTGATGTAAAAAGCAGTCAACAACCAACAAAGAGTTACATCAGATACGAAACACGTCAGAAGCGTGCGGATGCAAAGAAGGCCTTGAAAAACCTCCTCTTCAACAGTGGGTCCTCTAAAGGTTTATTTCATGACATTCAGAATGAAGATTTTTCGGGTAGCTCTCCTAAGAAACATAGATCAAGATCTTCTCGACATGCTGAGAAATCGCAACAAAAGAAAATGAAAAGGAAGTTTAAAAGAGAGAGTTTCTCTGAAGACTATGACAATGATCCTGAGAAAATCTTTCGGGCGACATTTGGAAAGAAATGGTACACCTGGTCTTTTAACTCACGACGTGACTCTTCTTACCAAGATTCAGAATCTGGATTTGAGTGGAGAGAGCAATCGAGCTGGAAAAGTAGAAGTAAAAGATGGGAGAATAGTAGTGACACAGAATCTGATGACAAATCACGTACTATAGGATCATTTTCTGATAGAACAATTCTCGGTCTGCCTCGGACAGGTCCTTTAAAGACCGAAGATGTTAAAAAAGCTTTCCATTTATCAGCTTTAAAATGGCATCCTGACAAGCATCAAGGCACTTCACAGGAAATCGCCGAAGAGAAATTTAAACTTTGTCTTAATGCCTACAAATCTCTGTGCAATGCATTATCCCCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

266

Amino Acids

30.86

Weight (kDa)

9.94

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 206 - 260 4e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 200
AclWI GGATC 2 cut(s) 398, 604
AcsI RAATTY 2 cut(s) 40, 740
AcuI CTGAAG 1 cut(s) 408
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 2 cut(s) 446, 589
AfiI CCNNNNNNNGG 1 cut(s) 635
AflIII ACRYGT 1 cut(s) 185
AgsI TTSAA 2 cut(s) 220, 235
AjiI CACGTC 2 cut(s) 188, 470
AjnI CCWGG 1 cut(s) 449
AluBI AGCT 5 cut(s) 68, 294, 525, 668, 683
AluI AGCT 5 cut(s) 68, 294, 525, 668, 683
AlwI GGATC 2 cut(s) 398, 604
AlwNI CAGNNNCTG 2 cut(s) 497, 572
AoxI GGCC 2 cut(s) 213, 792
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 2 cut(s) 40, 740
AspS9I GGNCC 3 cut(s) 129, 243, 638
AsuC2I CCSGG 1 cut(s) 791
AvaII GGWCC 3 cut(s) 129, 243, 638
BbsI GAAGAC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 77
BccI CCATC 2 cut(s) 9, 540
BciT130I CCWGG 1 cut(s) 451
BcnI CCSGG 1 cut(s) 791
BfmI CTRYAG 2 cut(s) 63, 591
BglII AGATCT 1 cut(s) 317
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 451, 791
Bme18I GGWCC 3 cut(s) 129, 243, 638
BmgBI CACGTC 2 cut(s) 188, 470
BmgT120I GGNCC 3 cut(s) 129, 243, 638
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 2 cut(s) 451, 791
BmsI GCATC 3 cut(s) 193, 703, 715
BpiI GAAGAC 1 cut(s) 396
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 791
BsaAI YACGTR 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 316
BsaJI CCNNGG 2 cut(s) 629, 789
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse3DI GCAATG 1 cut(s) 784
Bse8I GATNNNNATC 1 cut(s) 316
BseBI CCWGG 1 cut(s) 451
BseDI CCNNGG 2 cut(s) 629, 789
BseGI GGATG 2 cut(s) 208, 694
BseJI GATNNNNATC 1 cut(s) 316
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 2 cut(s) 324, 399
BseRI GAGGAG 2 cut(s) 23, 218
BseXI GCAGC 1 cut(s) 77
BshFI GGCC 2 cut(s) 215, 794
BsiSI CCGG 1 cut(s) 791
BslI CCNNNNNNNGG 1 cut(s) 635
BsnI GGCC 2 cut(s) 215, 794
Bsp143I GATC 4 cut(s) 311, 317, 403, 596
BspACI CCGC 1 cut(s) 200
BspANI GGCC 2 cut(s) 215, 794
BspCNI CTCAG 2 cut(s) 325, 400
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 2 cut(s) 398, 604
BsrDI GCAATG 1 cut(s) 784
BssECI CCNNGG 2 cut(s) 629, 789
BssMI GATC 4 cut(s) 311, 317, 403, 596
Bst2UI CCWGG 1 cut(s) 451
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 3 cut(s) 236, 481, 729
BstBAI YACGTR 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 300, 333, 408
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 2 cut(s) 208, 694
BstKTI GATC 4 cut(s) 314, 320, 406, 599
BstMBI GATC 4 cut(s) 311, 317, 403, 596
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 1 cut(s) 451
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 449, 789
BstSFI CTRYAG 2 cut(s) 63, 591
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 2 cut(s) 215, 794
BtrI CACGTC 2 cut(s) 188, 470
BtsCI GGATG 2 cut(s) 208, 694
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 198
CaiI CAGNNNCTG 2 cut(s) 497, 572
CciI TCATGA 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 129, 243, 638
CsiI ACCWGGT 1 cut(s) 449
Csp6I GTAC 2 cut(s) 445, 588
CviAII CATG 2 cut(s) 263, 329
CviJI RGCY 7 cut(s) 68, 215, 294, 525, 668, 683, 794
CviKI_1 RGCY 7 cut(s) 68, 215, 294, 525, 668, 683, 794
CviQI GTAC 2 cut(s) 445, 588
DdeI CTNAG 3 cut(s) 300, 333, 408
DpnI GATC 4 cut(s) 313, 319, 405, 598
DpnII GATC 4 cut(s) 311, 317, 403, 596
DraI TTTAAA 4 cut(s) 370, 645, 687, 745
DraIII CACNNNGTG 1 cut(s) 470
Eam1104I CTCTTC 3 cut(s) 236, 481, 729
EarI CTCTTC 3 cut(s) 236, 481, 729
Eco147I AGGCCT 1 cut(s) 215
Eco47I GGWCC 3 cut(s) 129, 243, 638
Eco57I CTGAAG 1 cut(s) 408
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoO109I RGGNCCY 2 cut(s) 243, 638
EcoRI GAATTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 784
FaeI CATG 2 cut(s) 266, 332
FaiI YATR 5 cut(s) 264, 309, 330, 396, 593
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 2 cut(s) 262, 328
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 2 cut(s) 215, 681
Fsp4HI GCNGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 2 cut(s) 215, 794
HapII CCGG 1 cut(s) 791
Hin1II CATG 2 cut(s) 266, 332
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 4 cut(s) 473, 488, 494, 569
HpaII CCGG 1 cut(s) 791
Hpy166II GTNNAC 2 cut(s) 152, 447
Hpy188I TCNGA 8 cut(s) 176, 192, 273, 388, 493, 574, 607, 633
Hpy188III TCNNGA 8 cut(s) 37, 263, 315, 324, 407, 465, 498, 698
Hpy8I GTNNAC 2 cut(s) 152, 447
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4IV ACGT 4 cut(s) 24, 187, 469, 586
HpyCH4V TGCA 5 cut(s) 4, 65, 206, 777, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 3 cut(s) 300, 333, 408
HpySE526I ACGT 4 cut(s) 24, 187, 469, 586
Hsp92II CATG 2 cut(s) 266, 332
Kzo9I GATC 4 cut(s) 311, 317, 403, 596
LmnI GCTCC 1 cut(s) 36
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 3 cut(s) 193, 703, 715
MabI ACCWGGT 1 cut(s) 449
MaeII ACGT 4 cut(s) 24, 187, 469, 586
MaeIII GTNAC 3 cut(s) 167, 470, 560
MalI GATC 4 cut(s) 313, 319, 405, 598
MboI GATC 4 cut(s) 311, 317, 403, 596
MboII GAAGA 8 cut(s) 130, 223, 290, 312, 401, 468, 665, 746
MflI RGATCY 1 cut(s) 317
MluCI AATT 3 cut(s) 40, 615, 740
MlyI GAGTC 1 cut(s) 467
MnlI CCTC 5 cut(s) 44, 236, 239, 256, 639
Mph1103I ATGCAT 1 cut(s) 784
MseI TTAA 7 cut(s) 369, 459, 644, 660, 686, 744, 756
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 2 cut(s) 451, 791
MvaI CCWGG 1 cut(s) 451
MwoI GCNNNNNNNGC 1 cut(s) 212
NciI CCSGG 1 cut(s) 791
NdeII GATC 4 cut(s) 311, 317, 403, 596
NlaIII CATG 2 cut(s) 266, 332
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 470, 560
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 1 cut(s) 332
PagI TCATGA 1 cut(s) 262
PceI AGGCCT 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PfeI GAWTC 3 cut(s) 488, 494, 569
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 587
PpuMI RGGWCCY 2 cut(s) 243, 638
Psp5II RGGWCCY 2 cut(s) 243, 638
Psp6I CCWGG 1 cut(s) 449
PspGI CCWGG 1 cut(s) 449
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 3 cut(s) 129, 243, 638
PspPPI RGGWCCY 2 cut(s) 243, 638
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 2 cut(s) 497, 572
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 446, 589
RsaNI GTAC 2 cut(s) 445, 588
SaqAI TTAA 7 cut(s) 369, 459, 644, 660, 686, 744, 756
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 4 cut(s) 311, 317, 403, 596
Sau96I GGNCC 3 cut(s) 129, 243, 638
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 451, 791
SexAI ACCWGGT 1 cut(s) 449
SfaNI GCATC 3 cut(s) 193, 703, 715
SfcI CTRYAG 2 cut(s) 63, 591
SinI GGWCC 3 cut(s) 129, 243, 638
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 3 cut(s) 40, 615, 740
SseBI AGGCCT 1 cut(s) 215
SsiI CCGC 1 cut(s) 200
StuI AGGCCT 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 449, 789
TaaI ACNGT 1 cut(s) 239
TaiI ACGT 4 cut(s) 27, 190, 472, 589
TaqI TCGA 2 cut(s) 325, 521
TaqII GACCGA 2 cut(s) 611, 665
TasI AATT 3 cut(s) 40, 615, 740
TfiI GAWTC 3 cut(s) 488, 494, 569
Tru1I TTAA 7 cut(s) 369, 459, 644, 660, 686, 744, 756
Tru9I TTAA 7 cut(s) 369, 459, 644, 660, 686, 744, 756
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 470, 560
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 2 cut(s) 470, 560
TspDTI ATGAA 6 cut(s) 33, 42, 72, 251, 291, 371
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 3 cut(s) 129, 243, 638
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 40, 740
XceI RCATGY 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.