Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005836

Physical Location & Seq

Genomic Coordinates Forward (+)

16225854 .. 16228978

Gene Structure 3125 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1057600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGCTGACGCAATCCCCAAACCCCATCAAACCCTCCCCCAAAATCTCACTCTCTTCCAACTTCTCCCCCTCCAACACTTTCTCACCATTACAATTTCCAAACTTTCCCTCCAAACCAAAAACCCTAAACTTCAAGCTCCTCTGCTCCCAATCCCCAACTCCAAAGCCGTCAGCACCACCGTCTTCCTCTCCAGTCGAAACCTTTATCAATGTATTACGGATCATACCCGACTGGGCAGACCGCACTCAAGAGCGTGGAATGAGACAGCGCAGAACACTTTATGACCATGAGAAATGGATGCAGCACAGAAGCTCCTACCGCCACCTCCGCCACTTGCTTTCCAGCCTTTCGTCCAGAGTCATCCTGTCTTTGATACCCCCGGTGATTGTTTTTACTTTGGTGGCTGTGGTGATTGCTAGCTATAATACAGCAGTTGATTTGGATTTGCTTCCGGGGTTTTTCCCGTTGTTGAGGTCCTCATCTTTGCCGTACCAGCTCACAGCGCCGGCATTGGCGCTGTTGCTGGTGTTCAGGACAGAGGCTTCGTATTCGAGATTTGAAGAAGGGCGGAAGGCTTGGACTGAGGTGATTGCAGGGGCTAATGATTTTGCGAGGCAGATCATTTCTAGTGTTGAGAATTCGGGTGATGCACAGCTCAAGAAGGCACTTTTGCAGTATATTATGGCCTTCCCTGTTGCGCTGAAGTGTCATGTCATTTATGGCTCAGACATTGGACGAGACCTCGAAGATTTGCTTGAAGTAGATGATCTGCTAGTTGTTTTGAACTCAAAACATCGACCCAGCTGTATTATTCAGTTCATCTCCCGGAGCCTTCAATTGCTAAAGTTGGAGGAATCGAAGAGAATTATGTTGCAATCAAAGATCTCTTGTTTCCATGAAGGAATTGGCGTATGTGAACAGCTAATCGGTACACCCATCCCTCTGTCATATACACGCTTGACCTCAAGGTTTCTAGTCCTCTGGAATCTCACTCTCCCTATCATACTATGGGATGATTGCCACTGGATTGTGGTTCCTGCTACTTTCATCAGTGCCGCTTCTTTATTCTGCATTGAAGAAGTGGGTGTTCTTATTGAGGAACCATTTCCGATGCTAGCCCTTGATGATCTTTGCAACTCAGTTCGGAACAATGTACAGGAGGCCCTTGCAAATGAAAGAGCAATCCATGCACGGCTTGCTGCAAAAGGAAAGATCCAGTCAGAGCCGCAGTTCCAAAAAGGCCAGCCTAGACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0006996 GO:0008150 GO:0008152 GO:0008308 GO:0008509 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009668 GO:0009987 GO:0010027 GO:0010109 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0015979 GO:0016020 GO:0016043 GO:0019222 GO:0019684 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031323 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0042548 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043467 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0061024 GO:0065007 GO:0071840 GO:0098656 GO:0098660 GO:0098661 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bestrophin

Protein Analysis

419

Amino Acids

46.97

Weight (kDa)

8.85

Isoelectric Point (pI)

58.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Bestrophin_2	PF25539	99 - 387	4.3e-86	Bacterial/Fungal Bestrophin protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005836)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45870 AT2G45870 AT2G45870 AT3G61320 AT3G61320 AT3G61320 AT3G61320
fragaria_vesca	FvH4_7g14170
malus_domestica	MD01G1057600.v1.1 MD01G1202300.v1.1 MD07G1139000.v1.1
prunus_persica	Prupe.2G168100_v2.0.a1 Prupe.2G168100_v2.0.a1 Prupe.2G168100_v2.0.a1
pyrus_communis	pycom01g08760 pycom07g13600
rosa_chinensis	RchiOBHm_Chr1g0355071
rosa_laevigata	RLG00000028179
rosa_multiflora	Rmu_sc0003115.1_g000030
rosa_roxburghii	Rroxscaffold_4G00300690
rosa_rugosa	Rorug01G0244900
rosa_samantha	Rh1AG257200 Rh1BG226500 Rh1CG240800 Rh1DG254100 Rh4DG156900
rosa_wichuraiana	Rw0G011210 Rw1G022680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	6 cut(s)	241, 319, 328, 568, 1056, 1226
AclWI	GGATC	2 cut(s)	227, 1207
AcsI	RAATTY	1 cut(s)	637
AcuI	CTGAAG	1 cut(s)	722
AfaI	GTAC	3 cut(s)	491, 931, 1155
AgsI	TTSAA	6 cut(s)	133, 560, 758, 784, 836, 1076
AluBI	AGCT	7 cut(s)	136, 312, 420, 496, 655, 804, 922
AluI	AGCT	7 cut(s)	136, 312, 420, 496, 655, 804, 922
Alw26I	GTCTC	2 cut(s)	256, 732
AlwI	GGATC	2 cut(s)	227, 1207
AoxI	GGCC	3 cut(s)	684, 1161, 1240
ApeKI	GCWGC	2 cut(s)	301, 1199
ApoI	RAATTY	1 cut(s)	637
ArsI	GACNNNNNNTTYG	2 cut(s)	526, 558
Asp700I	GAANNNNTTC	1 cut(s)	1104
AspLEI	GCGC	4 cut(s)	270, 505, 517, 700
AspS9I	GGNCC	2 cut(s)	474, 1162
AsuC2I	CCSGG	3 cut(s)	380, 453, 826
AsuHPI	GGTGA	5 cut(s)	75, 394, 421, 598, 656
AsuNHI	GCTAGC	2 cut(s)	416, 1114
AvaII	GGWCC	1 cut(s)	474
BbsI	GAAGAC	1 cut(s)	174
BbvI	GCAGC	2 cut(s)	313, 1186
BccI	CCATC	2 cut(s)	32, 944
BceAI	ACGGC	3 cut(s)	151, 472, 1208
BcnI	CCSGG	3 cut(s)	380, 453, 826
BcoDI	GTCTC	2 cut(s)	256, 732
BfaI	CTAG	6 cut(s)	417, 627, 773, 974, 1115, 1248
BfoI	RGCGCY	2 cut(s)	506, 518
BglII	AGATCT	1 cut(s)	882
BisI	GCNGC	4 cut(s)	302, 1056, 1200, 1226
BlsI	GCNGC	4 cut(s)	303, 1057, 1201, 1227
Bme1390I	CCNGG	3 cut(s)	380, 453, 826
Bme18I	GGWCC	1 cut(s)	474
BmgT120I	GGNCC	2 cut(s)	474, 1162
BmiI	GGNNCC	3 cut(s)	830, 1035, 1101
BmrFI	CCNGG	3 cut(s)	380, 453, 826
BmrI	ACTGGG	1 cut(s)	241
BmsI	GCATC	3 cut(s)	288, 637, 1101
BmtI	GCTAGC	2 cut(s)	420, 1118
BmuI	ACTGGG	1 cut(s)	241
BpiI	GAAGAC	1 cut(s)	174
BpmI	CTGGAG	1 cut(s)	174
BpuEI	CTTGAG	3 cut(s)	231, 641, 949
BpuMI	CCSGG	3 cut(s)	380, 453, 826
BsaI	GGTCTC	1 cut(s)	732
BsaJI	CCNNGG	2 cut(s)	378, 452
BsaXI	ACNNNNNCTCC	4 cut(s)	92, 122, 296, 326
Bse118I	RCCGGY	1 cut(s)	505
Bse1I	ACTGG	4 cut(s)	191, 236, 1028, 1216
BseDI	CCNNGG	2 cut(s)	378, 452
BseGI	GGATG	4 cut(s)	303, 360, 936, 1018
BseMII	CTCAG	3 cut(s)	573, 738, 1152
BseNI	ACTGG	4 cut(s)	191, 236, 1028, 1216
BseRI	GAGGAG	1 cut(s)	128
BseXI	GCAGC	2 cut(s)	313, 1186
BseYI	CCCAGC	1 cut(s)	800
BshFI	GGCC	3 cut(s)	686, 1163, 1242
BsiSI	CCGG	4 cut(s)	380, 452, 506, 826
BsmAI	GTCTC	2 cut(s)	256, 732
BsnI	GGCC	3 cut(s)	686, 1163, 1242
Bso31I	GGTCTC	1 cut(s)	732
Bsp1407I	TGTACA	1 cut(s)	1153
Bsp143I	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
BspACI	CCGC	6 cut(s)	241, 319, 328, 568, 1056, 1226
BspANI	GGCC	3 cut(s)	686, 1163, 1242
BspCNI	CTCAG	3 cut(s)	574, 737, 1151
BspLI	GGNNCC	3 cut(s)	830, 1035, 1101
BspOI	GCTAGC	2 cut(s)	420, 1118
BspPI	GGATC	2 cut(s)	227, 1207
BspTNI	GGTCTC	1 cut(s)	732
BsrFI	RCCGGY	1 cut(s)	505
BsrGI	TGTACA	1 cut(s)	1153
BsrI	ACTGG	4 cut(s)	191, 236, 1028, 1216
BssAI	RCCGGY	1 cut(s)	505
BssECI	CCNNGG	2 cut(s)	378, 452
BssMI	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
Bst4CI	ACNGT	1 cut(s)	180
Bst6I	CTCTTC	2 cut(s)	58, 854
BstAPI	GCANNNNNTGC	2 cut(s)	1187, 1196
BstAUI	TGTACA	1 cut(s)	1153
BstC8I	GCNNGC	5 cut(s)	418, 507, 1116, 1197, 1244
BstDEI	CTNAG	3 cut(s)	582, 724, 1138
BstF5I	GGATG	4 cut(s)	303, 360, 936, 1018
BstH2I	RGCGCY	2 cut(s)	506, 518
BstHHI	GCGC	4 cut(s)	270, 505, 517, 700
BstKTI	GATC	6 cut(s)	222, 621, 769, 885, 1129, 1215
BstMAI	GTCTC	2 cut(s)	256, 732
BstMBI	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
BstMWI	GCNNNNNNNGC	6 cut(s)	318, 327, 493, 502, 1187, 1196
BstSCI	CCNGG	3 cut(s)	378, 451, 824
BstV1I	GCAGC	2 cut(s)	313, 1186
BstV2I	GAAGAC	1 cut(s)	174
BstX2I	RGATCY	2 cut(s)	882, 1212
BstYI	RGATCY	2 cut(s)	882, 1212
BsuRI	GGCC	3 cut(s)	686, 1163, 1242
BtsCI	GGATG	4 cut(s)	303, 360, 936, 1018
BtsIMutI	CAGTG	2 cut(s)	1021, 1057
Cac8I	GCNNGC	5 cut(s)	418, 507, 1116, 1197, 1244
CfoI	GCGC	4 cut(s)	270, 505, 517, 700
Cfr10I	RCCGGY	1 cut(s)	505
Cfr13I	GGNCC	2 cut(s)	474, 1162
CseI	GACGC	1 cut(s)	16
Csp6I	GTAC	3 cut(s)	490, 930, 1154
CviAII	CATG	4 cut(s)	287, 710, 896, 1187
CviQI	GTAC	3 cut(s)	490, 930, 1154
DdeI	CTNAG	3 cut(s)	582, 724, 1138
DpnI	GATC	6 cut(s)	221, 620, 768, 884, 1128, 1214
DpnII	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
Eam1104I	CTCTTC	2 cut(s)	58, 854
EarI	CTCTTC	2 cut(s)	58, 854
EciI	GGCGGA	2 cut(s)	317, 583
Eco31I	GGTCTC	1 cut(s)	732
Eco47I	GGWCC	1 cut(s)	474
Eco57I	CTGAAG	1 cut(s)	722
EcoO109I	RGGNCCY	2 cut(s)	474, 1162
EcoRI	GAATTC	1 cut(s)	637
FaeI	CATG	4 cut(s)	290, 713, 899, 1190
FalI	AAGNNNNNCTT	2 cut(s)	738, 770
FatI	CATG	4 cut(s)	286, 709, 895, 1186
Fnu4HI	GCNGC	4 cut(s)	302, 1056, 1200, 1226
FokI	GGATG	4 cut(s)	310, 347, 923, 1025
Fsp4HI	GCNGC	4 cut(s)	302, 1056, 1200, 1226
FspBI	CTAG	6 cut(s)	417, 627, 773, 974, 1115, 1248
GlaI	GCGC	4 cut(s)	269, 504, 516, 699
GluI	GCNGC	4 cut(s)	302, 1056, 1200, 1226
GsaI	CCCAGC	1 cut(s)	804
GsuI	CTGGAG	1 cut(s)	174
HaeII	RGCGCY	2 cut(s)	506, 518
HaeIII	GGCC	3 cut(s)	686, 1163, 1242
HapII	CCGG	4 cut(s)	380, 452, 506, 826
HgaI	GACGC	1 cut(s)	16
HhaI	GCGC	4 cut(s)	270, 505, 517, 700
Hin1II	CATG	4 cut(s)	290, 713, 899, 1190
Hin6I	GCGC	4 cut(s)	268, 503, 515, 698
HinP1I	GCGC	4 cut(s)	268, 503, 515, 698
HinfI	GANTC	3 cut(s)	357, 854, 985
HpaII	CCGG	4 cut(s)	380, 452, 506, 826
HphI	GGTGA	5 cut(s)	75, 394, 421, 598, 656
Hpy166II	GTNNAC	2 cut(s)	917, 932
Hpy188I	TCNGA	4 cut(s)	727, 1110, 1146, 1222
Hpy188III	TCNNGA	6 cut(s)	248, 354, 532, 552, 658, 982
Hpy8I	GTNNAC	2 cut(s)	917, 932
HpyAV	CCTTC	6 cut(s)	557, 565, 655, 697, 842, 893
HpyCH4III	ACNGT	1 cut(s)	180
HpyF10VI	GCNNNNNNNGC	6 cut(s)	318, 327, 493, 502, 1187, 1196
HpyF3I	CTNAG	3 cut(s)	582, 724, 1138
Hsp92II	CATG	4 cut(s)	290, 713, 899, 1190
HspAI	GCGC	4 cut(s)	268, 503, 515, 698
KroI	GCCGGC	1 cut(s)	505
KroNI	GCCGGC	1 cut(s)	507
Kzo9I	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
LmnI	GCTCC	4 cut(s)	141, 149, 317, 828
Lsp1109I	GCAGC	2 cut(s)	313, 1186
LweI	GCATC	3 cut(s)	288, 637, 1101
MaeI	CTAG	6 cut(s)	417, 627, 773, 974, 1115, 1248
MalI	GATC	6 cut(s)	221, 620, 768, 884, 1128, 1214
MboI	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
MboII	GAAGA	6 cut(s)	45, 174, 572, 758, 871, 1088
MfeI	CAATTG	1 cut(s)	836
MflI	RGATCY	2 cut(s)	882, 1212
MluCI	AATT	5 cut(s)	92, 637, 836, 864, 903
MlyI	GAGTC	1 cut(s)	366
MmeI	TCCRAC	3 cut(s)	81, 96, 828
MroNI	GCCGGC	1 cut(s)	505
MroXI	GAANNNNTTC	1 cut(s)	1104
MspA1I	CMGCKG	1 cut(s)	804
MspI	CCGG	4 cut(s)	380, 452, 506, 826
MspR9I	CCNGG	3 cut(s)	380, 453, 826
MunI	CAATTG	1 cut(s)	836
MwoI	GCNNNNNNNGC	6 cut(s)	318, 327, 493, 502, 1187, 1196
NaeI	GCCGGC	1 cut(s)	507
NciI	CCSGG	3 cut(s)	380, 453, 826
NdeII	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
NgoMIV	GCCGGC	1 cut(s)	505
NheI	GCTAGC	2 cut(s)	416, 1114
NlaIII	CATG	4 cut(s)	290, 713, 899, 1190
NlaIV	GGNNCC	3 cut(s)	830, 1035, 1101
PdiI	GCCGGC	1 cut(s)	507
PdmI	GAANNNNTTC	1 cut(s)	1104
PfeI	GAWTC	2 cut(s)	854, 985
PfoI	TCCNGGA	1 cut(s)	824
PkrI	GCNGC	4 cut(s)	303, 1057, 1201, 1227
PleI	GAGTC	1 cut(s)	365
PpsI	GAGTC	1 cut(s)	365
PpuMI	RGGWCCY	1 cut(s)	474
Psp5II	RGGWCCY	1 cut(s)	474
PspFI	CCCAGC	1 cut(s)	800
PspN4I	GGNNCC	3 cut(s)	830, 1035, 1101
PspPI	GGNCC	2 cut(s)	474, 1162
PspPPI	RGGWCCY	1 cut(s)	474
PsuI	RGATCY	2 cut(s)	882, 1212
PvuII	CAGCTG	1 cut(s)	804
RsaI	GTAC	3 cut(s)	491, 931, 1155
RsaNI	GTAC	3 cut(s)	490, 930, 1154
SatI	GCNGC	4 cut(s)	302, 1056, 1200, 1226
Sau3AI	GATC	6 cut(s)	219, 618, 766, 882, 1126, 1212
Sau96I	GGNCC	2 cut(s)	474, 1162
SchI	GAGTC	1 cut(s)	366
ScrFI	CCNGG	3 cut(s)	380, 453, 826
SfaNI	GCATC	3 cut(s)	288, 637, 1101
SinI	GGWCC	1 cut(s)	474
SmlI	CTYRAG	3 cut(s)	246, 656, 964
SmoI	CTYRAG	3 cut(s)	246, 656, 964
Sse9I	AATT	5 cut(s)	92, 637, 836, 864, 903
SsiI	CCGC	6 cut(s)	241, 319, 328, 568, 1056, 1226
SspMI	CTAG	6 cut(s)	417, 627, 773, 974, 1115, 1248
StyD4I	CCNGG	3 cut(s)	378, 451, 824
TaaI	ACNGT	1 cut(s)	180
TaqI	TCGA	5 cut(s)	195, 551, 744, 796, 857
TasI	AATT	5 cut(s)	92, 637, 836, 864, 903
TatI	WGTACW	1 cut(s)	1153
TauI	GCSGC	2 cut(s)	1058, 1228
TfiI	GAWTC	2 cut(s)	854, 985
TscAI	CASTG	2 cut(s)	1028, 1057
TseI	GCWGC	2 cut(s)	301, 1199
TspDTI	ATGAA	4 cut(s)	808, 912, 1036, 1188
TspGWI	ACGGA	1 cut(s)	232
TspRI	CASTG	2 cut(s)	1028, 1057
VpaK11BI	GGWCC	1 cut(s)	474
XapI	RAATTY	1 cut(s)	637
XcmI	CCANNNNNNNNNTGG	1 cut(s)	902
XmnI	GAANNNNTTC	1 cut(s)	1104
XspI	CTAG	6 cut(s)	417, 627, 773, 974, 1115, 1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.