Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014940

Physical Location & Seq

Genomic Coordinates Reverse (-)

18445739 .. 18447924

Gene Structure 2186 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1078000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGGAGAAGAGAAAGAGCATGGAGCGGGAGCAGGAGAGTCTAACCAGTGAGCTAACGCAAGGGAAGGAGCTGGCTGAGCAGCTCATGAGCCACCTCCACCATTCTTCCTCAGAAGAAACAAGAGATTTTCTGATTTCAAAGATACTGTTTTCATATGAGAAGGCACTCTCACTGCTCACAGGGAGTGGGGATGGATCTGATGGAGAATCCAAACACATCACAGCTCGTGAAACTATGTTGGAATCACCAACTTCATTTGGCAATAGTAGCCCATTGAGTGAGATCTCTGACCAGGATTGCAAGCGCAAAAATGTCTTCAAGAAGACGAAAACAATGCCCAGCTGGACTGAGGAAGTGAAGGTTTCCTCTGGAACAGGGTTAGATGGTGGTCTTGATGATGGCTATAGTTGGAGAAAATATGGCCAAAAGAATATCCTTGGAGCTAATCATCCAAGAGGCTACTACAGATGCACACATCGTGGAACACAGGGTTGCGTAGCTACCAAGCAAGTTCAGAAATCAGATGCAGATCCAACAACCTTCGTGCTAACCTACAGGGGAGTCCATACTTGTAACAAAGCCTCTCAGTTGGCTAGGGTTAAAGAAGGCTTAAAAGGCAACGAAAATACAACCCTAGAAGTAAAAAAAGCGAAACAATTTTCACCAGAGATGTCATTTAGTTTTGGAAGAGCAGGGCTTAGAGTTAAAACTGAACATTTGGACGCTAGAGAGAAAGATATATTTTCACCATTCTCCTTCCCTTCCACTCCGATTGAATCCGAAATGGTTGGGGAACATATTTTCTGCTCAACGATGATGGAGAACAATTTGGTCGATAGCTATGCTCCAATATTTGAATCTCCACCAGCAACATTTGAGTCGGACTACTTGGCGGTGTCACCGTGCCATATGAGCAGTTTCGGACTAGCGCATGATGTGCAGACTTCAGAATCTGATCTCACTGAGATAATCTCAGCTCCAACTTCAGTCACCAATTCACCCATTGGGGATTTTGATTTCTCAATTGATGATTTTGAATTTCCCCCAGATTGTTTGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

354

Amino Acids

39.17

Weight (kDa)

5.3

Isoelectric Point (pI)

49.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	132 - 192	5e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014940)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46400
fragaria_vesca	FvH4_7g16150
malus_domestica	MD01G1078000.v1.1 MD07G1146900.v1.1
prunus_persica	Prupe.2G185100_v2.0.a1
pyrus_communis	pycom07g14630
rosa_chinensis	RchiOBHm_Chr1g0359091
rosa_laevigata	RLG00000027900
rosa_multiflora	Rmu_sc0020948.1_g000005
rosa_roxburghii	Rroxscaffold_4G00297350
rosa_rugosa	Rorug01G0267800
rosa_samantha	Rh1AG281300 Rh1BG248200 Rh1CG265800 Rh1DG276700
rosa_wichuraiana	Rw1G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	25
AciI	CCGC	2 cut(s)	25, 893
AclWI	GGATC	2 cut(s)	202, 524
AcoI	YGGCCR	1 cut(s)	421
AcsI	RAATTY	1 cut(s)	1037
AcuI	CTGAAG	2 cut(s)	930, 969
AdeI	CACNNNGTG	1 cut(s)	479
AgsI	TTSAA	5 cut(s)	138, 319, 776, 857, 1037
AjnI	CCWGG	1 cut(s)	291
AluBI	AGCT	9 cut(s)	52, 70, 82, 224, 342, 443, 500, 840, 977
AluI	AGCT	9 cut(s)	52, 70, 82, 224, 342, 443, 500, 840, 977
AlwI	GGATC	2 cut(s)	202, 524
AlwNI	CAGNNNCTG	1 cut(s)	953
AoxI	GGCC	1 cut(s)	421
ApeKI	GCWGC	1 cut(s)	79
ApoI	RAATTY	1 cut(s)	1037
AspLEI	GCGC	2 cut(s)	306, 931
AsuHPI	GGTGA	6 cut(s)	237, 654, 738, 891, 982, 990
BalI	TGGCCA	1 cut(s)	423
BauI	CACGAG	1 cut(s)	225
BbsI	GAAGAC	2 cut(s)	307, 329
BbvI	GCAGC	1 cut(s)	91
BccI	CCATC	5 cut(s)	185, 194, 377, 392, 811
BcgI	CGANNNNNNTGC	4 cut(s)	316, 350, 824, 858
BciT130I	CCWGG	1 cut(s)	293
BfaI	CTAG	4 cut(s)	594, 635, 726, 926
BfmI	CTRYAG	3 cut(s)	403, 463, 553
BglII	AGATCT	1 cut(s)	282
BisI	GCNGC	1 cut(s)	80
BlpI	GCTNAGC	1 cut(s)	75
BlsI	GCNGC	1 cut(s)	81
Bme1390I	CCNGG	1 cut(s)	293
BmrFI	CCNGG	1 cut(s)	293
BmsI	GCATC	2 cut(s)	458, 514
BpiI	GAAGAC	2 cut(s)	307, 329
BplI	GAGNNNNNCTC	2 cut(s)	956, 988
Bpu1102I	GCTNAGC	1 cut(s)	75
BsaBI	GATNNNNATC	1 cut(s)	528
BsaJI	CCNNGG	1 cut(s)	436
Bse1I	ACTGG	1 cut(s)	45
Bse8I	GATNNNNATC	1 cut(s)	528
BseBI	CCWGG	1 cut(s)	293
BseDI	CCNNGG	1 cut(s)	436
BseGI	GGATG	2 cut(s)	196, 448
BseJI	GATNNNNATC	1 cut(s)	528
BseMII	CTCAG	6 cut(s)	66, 123, 339, 599, 954, 987
BseNI	ACTGG	1 cut(s)	45
BseXI	GCAGC	1 cut(s)	91
BseYI	CCCAGC	1 cut(s)	338
BsgI	GTGCAG	1 cut(s)	959
BshFI	GGCC	1 cut(s)	423
BsnI	GGCC	1 cut(s)	423
Bsp143I	GATC	4 cut(s)	194, 282, 529, 955
Bsp1720I	GCTNAGC	1 cut(s)	75
BspACI	CCGC	2 cut(s)	25, 893
BspANI	GGCC	1 cut(s)	423
BspCNI	CTCAG	6 cut(s)	67, 122, 340, 598, 955, 986
BspHI	TCATGA	1 cut(s)	84
BspPI	GGATC	2 cut(s)	202, 524
BspQI	GCTCTTC	1 cut(s)	682
BsrBI	CCGCTC	1 cut(s)	25
BsrI	ACTGG	1 cut(s)	45
BssECI	CCNNGG	1 cut(s)	436
BssMI	GATC	4 cut(s)	194, 282, 529, 955
BssSI	CACGAG	1 cut(s)	225
BssT1I	CCWWGG	1 cut(s)	436
Bst2BI	CACGAG	1 cut(s)	225
Bst2UI	CCWGG	1 cut(s)	293
Bst4CI	ACNGT	2 cut(s)	147, 903
Bst6I	CTCTTC	2 cut(s)	2, 682
BstAPI	GCANNNNNTGC	1 cut(s)	937
BstC8I	GCNNGC	2 cut(s)	72, 302
BstDEI	CTNAG	7 cut(s)	75, 109, 348, 585, 698, 963, 973
BstF5I	GGATG	2 cut(s)	196, 448
BstHHI	GCGC	2 cut(s)	306, 931
BstKTI	GATC	4 cut(s)	197, 285, 532, 958
BstMBI	GATC	4 cut(s)	194, 282, 529, 955
BstMWI	GCNNNNNNNGC	5 cut(s)	76, 267, 615, 912, 937
BstNI	CCWGG	1 cut(s)	293
BstSCI	CCNGG	1 cut(s)	291
BstSFI	CTRYAG	3 cut(s)	403, 463, 553
BstV1I	GCAGC	1 cut(s)	91
BstV2I	GAAGAC	2 cut(s)	307, 329
BstX2I	RGATCY	3 cut(s)	194, 282, 529
BstYI	RGATCY	3 cut(s)	194, 282, 529
BsuRI	GGCC	1 cut(s)	423
BtsCI	GGATG	2 cut(s)	196, 448
BtsI	GCAGTG	1 cut(s)	170
BtsIMutI	CAGTG	3 cut(s)	52, 170, 960
Cac8I	GCNNGC	2 cut(s)	72, 302
CaiI	CAGNNNCTG	1 cut(s)	953
CciI	TCATGA	1 cut(s)	84
CfoI	GCGC	2 cut(s)	306, 931
CseI	GACGC	1 cut(s)	731
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CviAII	CATG	3 cut(s)	19, 85, 932
DdeI	CTNAG	7 cut(s)	75, 109, 348, 585, 698, 963, 973
DpnI	GATC	4 cut(s)	196, 284, 531, 957
DpnII	GATC	4 cut(s)	194, 282, 529, 955
DraIII	CACNNNGTG	1 cut(s)	479
EaeI	YGGCCR	1 cut(s)	421
Eam1104I	CTCTTC	2 cut(s)	2, 682
EarI	CTCTTC	2 cut(s)	2, 682
Eco130I	CCWWGG	1 cut(s)	436
Eco57I	CTGAAG	2 cut(s)	930, 969
EcoRII	CCWGG	1 cut(s)	291
EcoT14I	CCWWGG	1 cut(s)	436
ErhI	CCWWGG	1 cut(s)	436
FaeI	CATG	3 cut(s)	22, 88, 935
FatI	CATG	3 cut(s)	18, 84, 931
FauI	CCCGC	1 cut(s)	18
FauNDI	CATATG	2 cut(s)	154, 909
Fnu4HI	GCNGC	1 cut(s)	80
FokI	GGATG	2 cut(s)	203, 435
Fsp4HI	GCNGC	1 cut(s)	80
FspBI	CTAG	4 cut(s)	594, 635, 726, 926
GlaI	GCGC	2 cut(s)	305, 930
GluI	GCNGC	1 cut(s)	80
GsaI	CCCAGC	1 cut(s)	342
HaeIII	GGCC	1 cut(s)	423
HgaI	GACGC	1 cut(s)	731
HhaI	GCGC	2 cut(s)	306, 931
Hin1II	CATG	3 cut(s)	22, 88, 935
Hin6I	GCGC	2 cut(s)	304, 929
HinP1I	GCGC	2 cut(s)	304, 929
HinfI	GANTC	8 cut(s)	37, 206, 242, 561, 776, 857, 878, 950
HphI	GGTGA	6 cut(s)	237, 654, 738, 891, 982, 990
Hpy188III	TCNNGA	5 cut(s)	85, 227, 319, 369, 392
HpyAV	CCTTC	7 cut(s)	58, 154, 352, 550, 599, 766, 771
HpyCH4III	ACNGT	2 cut(s)	147, 903
HpyCH4V	TGCA	4 cut(s)	300, 471, 527, 940
HpyF10VI	GCNNNNNNNGC	5 cut(s)	76, 267, 615, 912, 937
HpyF3I	CTNAG	7 cut(s)	75, 109, 348, 585, 698, 963, 973
Hsp92II	CATG	3 cut(s)	22, 88, 935
HspAI	GCGC	2 cut(s)	304, 929
Kzo9I	GATC	4 cut(s)	194, 282, 529, 955
LguI	GCTCTTC	1 cut(s)	682
LmnI	GCTCC	6 cut(s)	22, 28, 67, 440, 850, 982
Lsp1109I	GCAGC	1 cut(s)	91
LweI	GCATC	2 cut(s)	458, 514
MaeI	CTAG	4 cut(s)	594, 635, 726, 926
MaeIII	GTNAC	3 cut(s)	572, 897, 988
MalI	GATC	4 cut(s)	196, 284, 531, 957
MbiI	CCGCTC	1 cut(s)	25
MboI	GATC	4 cut(s)	194, 282, 529, 955
MboII	GAAGA	6 cut(s)	19, 96, 125, 307, 334, 699
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	3 cut(s)	194, 282, 529
MlsI	TGGCCA	1 cut(s)	423
MluCI	AATT	5 cut(s)	656, 826, 994, 1023, 1037
MluNI	TGGCCA	1 cut(s)	423
MlyI	GAGTC	3 cut(s)	46, 570, 887
MmeI	TCCRAC	5 cut(s)	219, 389, 557, 861, 1004
MnlI	CCTC	6 cut(s)	104, 118, 343, 376, 449, 592
Mox20I	TGGCCA	1 cut(s)	423
MscI	TGGCCA	1 cut(s)	423
MseI	TTAA	4 cut(s)	600, 611, 705, 1060
Msp20I	TGGCCA	1 cut(s)	423
MspA1I	CMGCKG	1 cut(s)	342
MspR9I	CCNGG	1 cut(s)	293
MunI	CAATTG	1 cut(s)	1023
MvaI	CCWGG	1 cut(s)	293
MwoI	GCNNNNNNNGC	5 cut(s)	76, 267, 615, 912, 937
NdeI	CATATG	2 cut(s)	154, 909
NdeII	GATC	4 cut(s)	194, 282, 529, 955
NlaIII	CATG	3 cut(s)	22, 88, 935
NmuCI	GTSAC	2 cut(s)	897, 988
PagI	TCATGA	1 cut(s)	84
PciSI	GCTCTTC	1 cut(s)	682
PfeI	GAWTC	5 cut(s)	206, 242, 776, 857, 950
PkrI	GCNGC	1 cut(s)	81
PleI	GAGTC	3 cut(s)	45, 569, 886
PpsI	GAGTC	3 cut(s)	45, 569, 886
Psp6I	CCWGG	1 cut(s)	291
PspFI	CCCAGC	1 cut(s)	338
PspGI	CCWGG	1 cut(s)	291
PstNI	CAGNNNCTG	1 cut(s)	953
PsuI	RGATCY	3 cut(s)	194, 282, 529
PvuII	CAGCTG	1 cut(s)	342
SapI	GCTCTTC	1 cut(s)	682
SaqAI	TTAA	4 cut(s)	600, 611, 705, 1060
SatI	GCNGC	1 cut(s)	80
Sau3AI	GATC	4 cut(s)	194, 282, 529, 955
SchI	GAGTC	3 cut(s)	46, 570, 887
ScrFI	CCNGG	1 cut(s)	293
SfaNI	GCATC	2 cut(s)	458, 514
SfcI	CTRYAG	3 cut(s)	403, 463, 553
Sse9I	AATT	5 cut(s)	656, 826, 994, 1023, 1037
SsiI	CCGC	2 cut(s)	25, 893
SspI	AATATT	1 cut(s)	852
SspMI	CTAG	4 cut(s)	594, 635, 726, 926
StyD4I	CCNGG	1 cut(s)	291
StyI	CCWWGG	1 cut(s)	436
TaaI	ACNGT	2 cut(s)	147, 903
TaqI	TCGA	1 cut(s)	834
TasI	AATT	5 cut(s)	656, 826, 994, 1023, 1037
TfiI	GAWTC	5 cut(s)	206, 242, 776, 857, 950
Tru1I	TTAA	4 cut(s)	600, 611, 705, 1060
Tru9I	TTAA	4 cut(s)	600, 611, 705, 1060
TscAI	CASTG	3 cut(s)	52, 177, 967
TseFI	GTSAC	2 cut(s)	897, 988
TseI	GCWGC	1 cut(s)	79
Tsp45I	GTSAC	2 cut(s)	897, 988
TspDTI	ATGAA	2 cut(s)	141, 243
TspRI	CASTG	3 cut(s)	52, 177, 967
XapI	RAATTY	1 cut(s)	1037
XspI	CTAG	4 cut(s)	594, 635, 726, 926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.