Rw1G025020
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014940
Physical Location & Seq
Reverse (-)
51558836 .. 51561547
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025020.1

Sequence Viewer

Length: 1050 bp
ATGGAGAAGAGGAAGAGTATGATGGGTAATTGGGAGCAAAATATTCTAAAAAGTGAGTTAACCCAGGGGAAGGAGCTGGCAGAGAAGCTCATGAATCATCTCCACCACTCTTCATCATCTGAAGAAGGAGAATTCTTGATTTCAAAGCTACTATCTTCATATGAAAAGGTACTCTCTGTGCTCACAAGGGATGTTGGCTCTGAAGGAGAGTCCAAGCAAATCCAAGTGTTGGACTCACCTTGTTCATTTGGGAATAGCAGTAGCCCATTGAGTGAGATCTCTGACCACGATTGCAAGAACAAGAATGTCTTCAAGAAAAGGAAAACAATGCCCAAGAGGACTGCCGAAGTGAAGGTTTGCTCTGGAACAGGACAAGATGGCTCTCTTGATGATGGCTATAGTTGGAGAAAATATGGCCAAAAGGATATCCTTGGTGCTAATTACCCAAGAGGCTACTATCGATGCACACATAGAAACACACAAGGTTGCCTAGCCACAAAGCAAGTTCAAAAATCAGATGCAGACCCAACAATCTTTATGGTAACTTATACAGGAGAACATACTTGTCACCTAGTCGCTCAGTTGAACGGAAAGCAGAATGTTTCTCTTAAACAAGAAGCAGGAACACCGAGACAGACACCAGAAAAATCATTCAGTTTTGGGCTTAGAGTTAAAACTGAGAATTTGGACACTAGGGGGGAGGGAAAGGAGATATTTCCATCATTTTCCTTCCCTCCCACGCCAATAGAGTCTGAAAACAATGTGGTGGAAGACCATGTTTTCGCTGCAACTGAGAACGATTTCCTGGGTGGTAGCTACGCTCAAACATTCGGGTCTCCGGCAACATTCGAATCAGACTACTTTGCGGCAGTGTCACCGTGCCACTTTGTACTAGGCAATGATGTGCAAACTTCAGATTCTGATCTCACTCATGAGATACTATCGGCCTCAACTTCTGTGGCCAACTCACCAATTGGGGATTTCGATTTCTCACTAGAGGATTTGGATTTTTACCCATTTGAAAACAACCCAGAATCTACTTTTCCCTAG

Protein Analysis

349

Amino Acids

38.75

Weight (kDa)

5.21

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 190 1.8e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46400
fragaria_vesca FvH4_7g16150
malus_domestica MD01G1078000.v1.1 MD07G1146900.v1.1
prunus_persica Prupe.2G185100_v2.0.a1
pyrus_communis pycom07g14630
rosa_chinensis RchiOBHm_Chr1g0359091
rosa_laevigata RLG00000027900
rosa_multiflora Rmu_sc0020948.1_g000005
rosa_roxburghii Rroxscaffold_4G00297350
rosa_rugosa Rorug01G0267800
rosa_samantha Rh1AG281300 Rh1BG248200 Rh1CG265800 Rh1DG276700
rosa_wichuraiana Rw1G025020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 229
AciI CCGC 1 cut(s) 866
AcoI YGGCCR 2 cut(s) 415, 960
AcsI RAATTY 2 cut(s) 131, 682
AcuI CTGAAG 3 cut(s) 141, 222, 897
AfaI GTAC 2 cut(s) 171, 891
AfiI CCNNNNNNNGG 2 cut(s) 70, 229
AgsI TTSAA 5 cut(s) 144, 313, 509, 586, 1022
AjnI CCWGG 2 cut(s) 63, 804
AluBI AGCT 4 cut(s) 76, 88, 148, 816
AluI AGCT 4 cut(s) 76, 88, 148, 816
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 625, 840
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 3 cut(s) 415, 945, 960
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 2 cut(s) 131, 682
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
Asp700I GAANNNNTTC 2 cut(s) 308, 800
AsuHPI GGTGA 4 cut(s) 228, 560, 867, 960
AsuII TTCGAA 1 cut(s) 849
BalI TGGCCA 2 cut(s) 417, 962
BbsI GAAGAC 2 cut(s) 301, 777
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 772
BccI CCATC 4 cut(s) 16, 371, 386, 727
BciT130I CCWGG 2 cut(s) 65, 806
BcoDI GTCTC 2 cut(s) 625, 840
BfaI CTAG 6 cut(s) 491, 572, 693, 893, 995, 1048
BfmI CTRYAG 1 cut(s) 397
BglII AGATCT 1 cut(s) 276
BisI GCNGC 2 cut(s) 786, 867
BlsI GCNGC 2 cut(s) 787, 868
Bme1390I CCNGG 2 cut(s) 65, 806
BmrFI CCNGG 2 cut(s) 65, 806
BmsI GCATC 2 cut(s) 452, 508
BpiI GAAGAC 2 cut(s) 301, 777
Bpu14I TTCGAA 1 cut(s) 849
Bsa29I ATCGAT 1 cut(s) 460
BsaBI GATNNNNATC 1 cut(s) 921
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 4 cut(s) 63, 64, 430, 805
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 229
Bse3DI GCAATG 1 cut(s) 904
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 2 cut(s) 65, 806
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 4 cut(s) 63, 64, 430, 805
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 2 cut(s) 70, 229
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 3 cut(s) 593, 669, 783
BseXI GCAGC 1 cut(s) 772
BshFI GGCC 3 cut(s) 417, 947, 962
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 839
BslI CCNNNNNNNGG 2 cut(s) 70, 229
BsmAI GTCTC 2 cut(s) 625, 840
BsnI GGCC 3 cut(s) 417, 947, 962
Bso31I GGTCTC 1 cut(s) 840
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 276, 922
BspACI CCGC 1 cut(s) 866
BspANI GGCC 3 cut(s) 417, 947, 962
BspCNI CTCAG 3 cut(s) 592, 670, 784
BspDI ATCGAT 1 cut(s) 460
BspHI TCATGA 2 cut(s) 90, 931
BspT104I TTCGAA 1 cut(s) 849
BspTNI GGTCTC 1 cut(s) 840
BsrDI GCAATG 1 cut(s) 904
BssECI CCNNGG 4 cut(s) 63, 64, 430, 805
BssMI GATC 2 cut(s) 276, 922
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 65, 806
Bst4CI ACNGT 1 cut(s) 879
Bst6I CTCTTC 3 cut(s) 2, 8, 115
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 4 cut(s) 579, 665, 678, 792
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 2 cut(s) 279, 925
BstMAI GTCTC 2 cut(s) 625, 840
BstMBI GATC 2 cut(s) 276, 922
BstNI CCWGG 2 cut(s) 65, 806
BstSCI CCNGG 2 cut(s) 63, 804
BstSFI CTRYAG 1 cut(s) 397
BstV1I GCAGC 1 cut(s) 772
BstV2I GAAGAC 2 cut(s) 301, 777
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 3 cut(s) 417, 947, 962
BsuTUI ATCGAT 1 cut(s) 460
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 1 cut(s) 876
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 2 cut(s) 90, 931
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 2 cut(s) 170, 890
CspCI CAANNNNNGTGG 2 cut(s) 939, 974
CviAII CATG 3 cut(s) 91, 776, 932
CviQI GTAC 2 cut(s) 170, 890
DdeI CTNAG 4 cut(s) 579, 665, 678, 792
DpnI GATC 2 cut(s) 278, 924
DpnII GATC 2 cut(s) 276, 922
EaeI YGGCCR 2 cut(s) 415, 960
Eam1104I CTCTTC 3 cut(s) 2, 8, 115
EarI CTCTTC 3 cut(s) 2, 8, 115
Eco130I CCWWGG 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 840
Eco32I GATATC 1 cut(s) 427
Eco57I CTGAAG 3 cut(s) 141, 222, 897
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 63, 804
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 3 cut(s) 94, 779, 935
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FatI CATG 3 cut(s) 90, 775, 931
FauNDI CATATG 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 786, 867
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 786, 867
FspBI CTAG 6 cut(s) 491, 572, 693, 893, 995, 1048
GluI GCNGC 2 cut(s) 786, 867
HaeIII GGCC 3 cut(s) 417, 947, 962
HapII CCGG 1 cut(s) 839
Hin1II CATG 3 cut(s) 94, 779, 935
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HinfI GANTC 7 cut(s) 94, 209, 233, 749, 851, 917, 1034
HpaI GTTAAC 1 cut(s) 60
HpaII CCGG 1 cut(s) 839
HphI GGTGA 4 cut(s) 228, 560, 867, 960
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 8 cut(s) 121, 202, 283, 517, 754, 856, 916, 922
Hpy188III TCNNGA 6 cut(s) 91, 136, 313, 363, 386, 932
Hpy8I GTNNAC 1 cut(s) 60
HpyAV CCTTC 5 cut(s) 64, 119, 197, 346, 739
HpyCH4III ACNGT 1 cut(s) 879
HpyCH4V TGCA 5 cut(s) 294, 465, 521, 788, 907
HpyF3I CTNAG 4 cut(s) 579, 665, 678, 792
Hsp92II CATG 3 cut(s) 94, 779, 935
KspAI GTTAAC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 276, 922
LmnI GCTCC 2 cut(s) 34, 73
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 452, 508
MaeI CTAG 6 cut(s) 491, 572, 693, 893, 995, 1048
MaeIII GTNAC 3 cut(s) 541, 566, 873
MalI GATC 2 cut(s) 278, 924
MboI GATC 2 cut(s) 276, 922
MboII GAAGA 7 cut(s) 19, 25, 102, 134, 147, 301, 782
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 2 cut(s) 417, 962
MluCI AATT 5 cut(s) 28, 131, 439, 682, 972
MluNI TGGCCA 2 cut(s) 417, 962
MlyI GAGTC 3 cut(s) 218, 227, 758
MmeI TCCRAC 2 cut(s) 210, 383
MnlI CCTC 7 cut(s) 3, 330, 443, 694, 744, 958, 991
Mox20I TGGCCA 2 cut(s) 417, 962
MroXI GAANNNNTTC 2 cut(s) 308, 800
MscI TGGCCA 2 cut(s) 417, 962
MseI TTAA 3 cut(s) 59, 609, 672
Msp20I TGGCCA 2 cut(s) 417, 962
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 2 cut(s) 65, 806
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 2 cut(s) 65, 806
NdeI CATATG 1 cut(s) 160
NdeII GATC 2 cut(s) 276, 922
NlaIII CATG 3 cut(s) 94, 779, 935
NmuCI GTSAC 2 cut(s) 566, 873
NspV TTCGAA 1 cut(s) 849
PagI TCATGA 2 cut(s) 90, 931
PasI CCCWGGG 1 cut(s) 64
PdmI GAANNNNTTC 2 cut(s) 308, 800
PfeI GAWTC 4 cut(s) 94, 851, 917, 1034
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 2 cut(s) 787, 868
PleI GAGTC 3 cut(s) 217, 227, 757
PpsI GAGTC 3 cut(s) 217, 227, 757
Psp6I CCWGG 2 cut(s) 63, 804
PspGI CCWGG 2 cut(s) 63, 804
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 276
RsaI GTAC 2 cut(s) 171, 891
RsaNI GTAC 2 cut(s) 170, 890
SaqAI TTAA 3 cut(s) 59, 609, 672
SatI GCNGC 2 cut(s) 786, 867
Sau3AI GATC 2 cut(s) 276, 922
SchI GAGTC 3 cut(s) 218, 227, 758
ScrFI CCNGG 2 cut(s) 65, 806
SduI GDGCHC 1 cut(s) 183
SetI ASST 9 cut(s) 78, 90, 150, 171, 241, 357, 487, 573, 818
SfaNI GCATC 2 cut(s) 452, 508
SfcI CTRYAG 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 849
Sse9I AATT 5 cut(s) 28, 131, 439, 682, 972
SsiI CCGC 1 cut(s) 866
SspI AATATT 1 cut(s) 43
SspMI CTAG 6 cut(s) 491, 572, 693, 893, 995, 1048
StyD4I CCNGG 2 cut(s) 63, 804
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 1 cut(s) 879
TaqI TCGA 3 cut(s) 460, 849, 984
TasI AATT 5 cut(s) 28, 131, 439, 682, 972
TatI WGTACW 1 cut(s) 889
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 4 cut(s) 94, 851, 917, 1034
Tru1I TTAA 3 cut(s) 59, 609, 672
Tru9I TTAA 3 cut(s) 59, 609, 672
TscAI CASTG 1 cut(s) 876
TseFI GTSAC 2 cut(s) 566, 873
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 2 cut(s) 566, 873
TspDTI ATGAA 5 cut(s) 102, 107, 147, 177, 234
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 1 cut(s) 876
Van91I CCANNNNNTGG 1 cut(s) 229
XapI RAATTY 2 cut(s) 131, 682
XmnI GAANNNNTTC 2 cut(s) 308, 800
XspI CTAG 6 cut(s) 491, 572, 693, 893, 995, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.