Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Reverse (-)

18655248 .. 18659581

Gene Structure 4334 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1079300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGAAAACGACGTCTCTTTCGGCCACTTTCATGCCCGTCAGACTTTTACCCCAACTCCGACTCCTCAGCGCCTCTCTTCCGGCGGCTCATTTCCCGCCCATGAAATTCGCGCCCTCCATTTCCTCCTCTCGGGCTGTACCCATTTTGTCGCGGGTCTTCCCCGTAAGGCTCAGGTACCTAGGGTTTCGCCGTTCATTGCAGACTGAGCGGGCATTCTCCACTCGGGCGTTCCGGGGATCCAGGACAGGGTCCGGGTACGAGAGAAGGGCGGGTGGAGCTTCGAAGAGTCTGATTGAGGACGAGGCTGAGCTCGGTGACTGGGTCAGCGAGTTAAAGAGTGACTCGCGCGGCGAACCGGAGGGCCGTAGGGGGACGGTTAGAGAGAGGGCGACCGATGGGGAGTCTTATCCGATAAAGAGGAGCAGGAGAGATAGTGACTCTGGAGATTTTGGTGACTCGAGAAGGCGAGAGTTTCGGTCCCCCAATCAGTCCTCGTTTTCGAGAAATCCGGGGATGAGTAAGAGGTTCGATAACAATTCCGGGAGTGATAGGAAAGAGAGATCATTTCCACCCAGAAACAATCGTGGGAATCCGAATTCAAATGGCGATTTTGGAGATTCGAGTAGGCGAAGATTTCCGGCCCAAAATGATTCCTTTTCGAGAAATGCAGGGGCAAGCAAGCGGTTTGATAAGAAATTTCAGAGTGATGACGACCAGGAAGACAGATTTCACCGTAGAAACAATCGTGGGGATTCAAATTCATTTTCTAAAGGGAGTTTGAGTTCAAAGGTGAAAGCGGAATCTTTTACCAGGAATAGAGGTGGAAGAGATTTGGGTAAGGAAATGCGTGTAATGGATGATACTGAAGAGGAGGAGGTAAAACAGTTCGGTGTTATTGGGGATTTTCTTAGTGAGGAAGAGAGTGATACTGCTACTTCTGATGATGATGGTTTTGGAGTATTAAGAGAAAAAAATGCAGCTTCTTTGTTTCGTGGTTCGGAGGATGAAGTGAGCAAAAAGGTTCTGCCGAAAAGTTCAGCTGGAAGCTCCGACTCTTATTTGAGTGAATCACGGTTTGATCAATGTTCCGTCTCTCCGCTGTCCTTGAAAGGAATCAAGGATGCAGGATATGAAAAGATGACTGTAGTACAAGAAGCTACTCTTCCAGTAATACTGAAAGGCAAGGATGTCCTGGCCAAGGCTAGAACGGGCACTGGAAAAACTGTGGCGTTCTTGCTTCCATCAATTGAAGTTGTTGTAAATTCACCTCCTATCACCCGTGATAATAAGCGACCCCCAATTTTTGTACTTGTTATATGCCCAACTCGGGAGTTGGCCAGTCAAGCTGCTACAGAAGCCAATAAGTTGTTGAAATATCATCCTTCTATTGGTGTCCAAGTTGTGATAGGTGGTACAAGACTTGCTCTAGAACAAAAACGCATCCAAGCCAACCCTTGCCAGATTCTTGTTGCTACACCTGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTTGCAACTAAGCTGATGGGTGTCAAAGTCCTTATACTTGATGAAGCTGATCATTTACTGGATATGGGATTCCGTAAAGATATCGAAAGGATTATATCAGCTGTTCCCAAACAACGGCAGACGCTTTTGTTTTCCGCCACTGTTCCTGAAGAGGTCCGTCAAATCTGTCATATTGCTTTGAAAAGAGATCACGAATATATCAATACAGTTGAAGAAGGCAGTGAAGAGACACATGCACAGGTTGCACAGACGCATTTGGTCGCTCCATTGGACAGCCATTTTTCCATAGTATATAGTCTTCTTAAAAATCATATTGCAGATGACGTTGACTATAAGGTTCTTGTATTCTGCACTACCGCAATGGTCACACGACTGGTTGCAGACCTTCTTGGCAAGCTGAACTTGAATGTCAGAGAGATCCATTCTAGAAAGCCCCAGAGTTATAGAACCAGGGTTTCTGATGAATTCCGGAAATCAAAGGGTCTTATCCTTGTGACTTCTGATGTATCTGCACGCGGGGTTGATTATCCAGATGTTTCCCTAGTCATACAGGTGGGTGTGCCAGCCGATAGACAACAGTATATACATCGACTTGGAAGAACTGGTCGTAAAGGCAAAGTGGGGCAAGGAATATTGTTACTGGCTCCTTGGGAGGACTTCTTCTTGTCCAATATTAAGGATTTGCCAATAACCAAGGCTTCGGTACCTTCAGTCGATCCAGATACTATGAAGAAGGTGGAACGAGCACTGTCCAAGGTGGAGATTAAGAACAAAGAAGCAGCATACCAAGCGTGGCTCGGGTATTACAATTCGAACAAGAAAGTGGGGCGCGATAAGCATAGGCTTGTGGAGCTTGCTAACGAATTTAGCGGAAGCATGGGGCTGGACAACCCTCCCGCCATTCCCAAGCTTGTTCTCGGCAAGATGGGGCTTAAGAATGTCCCTGGATTGCGTTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

826

Amino Acids

91.71

Weight (kDa)

9.65

Isoelectric Point (pI)

51.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	382 - 559	1.5e-45	DEAD/DEAH box helicase
ResIII	PF04851	394 - 554	2.1e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	605 - 710	5.8e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc36I	ACCTGC	1 cut(s)	1502
Acc65I	GGTACC	2 cut(s)	174, 2218
AccB1I	GGYRCC	2 cut(s)	174, 2218
AccBSI	CCGCTC	1 cut(s)	208
AccII	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
AccIII	TCCGGA	1 cut(s)	1983
AclWI	GGATC	4 cut(s)	231, 244, 1927, 2225
AcoI	YGGCCR	3 cut(s)	21, 1194, 1335
AcsI	RAATTY	7 cut(s)	104, 595, 695, 757, 1261, 1979, 2378
AcuI	CTGAAG	3 cut(s)	885, 1683, 2208
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	7 cut(s)	138, 176, 257, 1149, 1308, 1414, 2220
AfiI	CCNNNNNNNGG	6 cut(s)	129, 246, 1198, 1327, 1388, 1629
AflII	CTTAAG	1 cut(s)	2447
AgsI	TTSAA	9 cut(s)	600, 756, 786, 1108, 1250, 1372, 1696, 1727, 1921
AjnI	CCWGG	7 cut(s)	239, 714, 809, 1191, 1477, 1964, 2458
AloI	GAACNNNNNNTCC	2 cut(s)	766, 798
Alw21I	GWGCWC	2 cut(s)	312, 2263
Alw26I	GTCTC	3 cut(s)	18, 1096, 1736
AlwI	GGATC	4 cut(s)	231, 244, 1927, 2225
Ama87I	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
Aor13HI	TCCGGA	1 cut(s)	1983
AoxI	GGCC	5 cut(s)	21, 361, 639, 1194, 1335
ApeKI	GCWGC	3 cut(s)	977, 1346, 2294
ApoI	RAATTY	7 cut(s)	104, 595, 695, 757, 1261, 1979, 2378
ArsI	GACNNNNNNTTYG	1 cut(s)	33
Asp718I	GGTACC	2 cut(s)	174, 2218
AspA2I	CCTAGG	1 cut(s)	178
AspLEI	GCGC	4 cut(s)	71, 112, 348, 2346
AspS9I	GGNCC	5 cut(s)	249, 361, 477, 640, 1669
AsuC2I	CCSGG	4 cut(s)	233, 253, 510, 541
AsuHPI	GGTGA	6 cut(s)	326, 464, 722, 802, 1257, 1267
AsuII	TTCGAA	2 cut(s)	281, 2327
AvaI	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
AvaII	GGWCC	3 cut(s)	249, 477, 1669
AvrII	CCTAGG	1 cut(s)	178
BaeGI	GKGCMC	1 cut(s)	1214
BalI	TGGCCA	2 cut(s)	1196, 1337
BamHI	GGATCC	1 cut(s)	236
BanI	GGYRCC	2 cut(s)	174, 2218
BanII	GRGCYC	1 cut(s)	312
BarI	GAAGNNNNNNTAC	2 cut(s)	1797, 1829
BbsI	GAAGAC	3 cut(s)	148, 726, 1805
Bbv12I	GWGCWC	2 cut(s)	312, 2263
BbvCI	CCTCAGC	1 cut(s)	65
BbvI	GCAGC	3 cut(s)	989, 1333, 2306
BccI	CCATC	5 cut(s)	389, 941, 1249, 1525, 2434
BceAI	ACGGC	3 cut(s)	174, 348, 1646
BciT130I	CCWGG	7 cut(s)	241, 716, 811, 1193, 1479, 1966, 2460
BclI	TGATCA	2 cut(s)	1078, 1564
BcnI	CCSGG	4 cut(s)	233, 253, 510, 541
BcoDI	GTCTC	3 cut(s)	18, 1096, 1736
BfaI	CTAG	5 cut(s)	179, 1203, 1427, 1941, 2057
BfmI	CTRYAG	2 cut(s)	1143, 1350
BfoI	RGCGCY	1 cut(s)	72
BfrI	CTTAAG	1 cut(s)	2447
BfuAI	ACCTGC	1 cut(s)	1502
BisI	GCNGC	5 cut(s)	84, 349, 978, 1347, 2295
BlnI	CCTAGG	1 cut(s)	178
BlpI	GCTNAGC	1 cut(s)	306
BlsI	GCNGC	5 cut(s)	85, 350, 979, 1348, 2296
Bme18I	GGWCC	3 cut(s)	249, 477, 1669
BmeT110I	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
BmgT120I	GGNCC	5 cut(s)	249, 361, 477, 640, 1669
BmiI	GGNNCC	6 cut(s)	176, 238, 250, 479, 2160, 2220
BmrI	ACTGGG	1 cut(s)	328
BmsI	GCATC	2 cut(s)	1111, 1449
BmuI	ACTGGG	1 cut(s)	328
BpiI	GAAGAC	3 cut(s)	148, 726, 1805
BpmI	CTGGAG	1 cut(s)	462
Bpu10I	CCTNAGC	2 cut(s)	65, 170
Bpu1102I	GCTNAGC	1 cut(s)	306
Bpu14I	TTCGAA	2 cut(s)	281, 2327
BpuMI	CCSGG	4 cut(s)	233, 253, 510, 541
BsaHI	GRCGYC	1 cut(s)	11
BsaJI	CCNNGG	9 cut(s)	178, 232, 509, 1197, 1965, 2162, 2208, 2268, 2458
BsaWI	WCCGGW	2 cut(s)	355, 1983
BsaXI	ACNNNNNCTCC	8 cut(s)	39, 45, 69, 75, 418, 448, 766, 796
Bsc4I	CCNNNNNNNGG	6 cut(s)	129, 246, 1198, 1327, 1388, 1629
Bse1I	ACTGG	8 cut(s)	323, 1166, 1219, 1338, 1578, 1893, 2122, 2160
Bse3DI	GCAATG	2 cut(s)	194, 1881
BseAI	TCCGGA	1 cut(s)	1983
BseBI	CCWGG	7 cut(s)	241, 716, 811, 1193, 1479, 1966, 2460
BseDI	CCNNGG	9 cut(s)	178, 232, 509, 1197, 1965, 2162, 2208, 2268, 2458
BseGI	GGATG	7 cut(s)	519, 862, 1009, 1126, 1192, 1378, 1440
BseLI	CCNNNNNNNGG	6 cut(s)	129, 246, 1198, 1327, 1388, 1629
BseMI	GCAATG	2 cut(s)	194, 1881
BseMII	CTCAG	4 cut(s)	79, 184, 195, 297
BseNI	ACTGG	8 cut(s)	323, 1166, 1219, 1338, 1578, 1893, 2122, 2160
BseRI	GAGGAG	5 cut(s)	53, 115, 433, 884, 887
BseSI	GKGCMC	1 cut(s)	1214
BseXI	GCAGC	3 cut(s)	989, 1333, 2306
BsgI	GTGCAG	2 cut(s)	1849, 2010
Bsh1236I	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
Bsh1285I	CGRYCG	1 cut(s)	393
BshFI	GGCC	5 cut(s)	23, 363, 641, 1196, 1337
BshNI	GGYRCC	2 cut(s)	174, 2218
BsiEI	CGRYCG	1 cut(s)	393
BsiHKAI	GWGCWC	2 cut(s)	312, 2263
BsiHKCI	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
BsiSI	CCGG	8 cut(s)	80, 232, 252, 356, 509, 540, 638, 1984
BslFI	GGGAC	3 cut(s)	385, 463, 2441
BslI	CCNNNNNNNGG	6 cut(s)	129, 246, 1198, 1327, 1388, 1629
BsmAI	GTCTC	3 cut(s)	18, 1096, 1736
BsmBI	CGTCTC	2 cut(s)	18, 1096
BsmFI	GGGAC	3 cut(s)	385, 463, 2441
BsmI	GAATGC	1 cut(s)	212
BsnI	GGCC	5 cut(s)	23, 363, 641, 1196, 1337
BsoBI	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
Bsp119I	TTCGAA	2 cut(s)	281, 2327
Bsp1286I	GDGCHC	3 cut(s)	312, 1214, 2263
Bsp13I	TCCGGA	1 cut(s)	1983
Bsp143I	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
Bsp1720I	GCTNAGC	1 cut(s)	306
BspANI	GGCC	5 cut(s)	23, 363, 641, 1196, 1337
BspCNI	CTCAG	4 cut(s)	78, 183, 196, 298
BspEI	TCCGGA	1 cut(s)	1983
BspFNI	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
BspLI	GGNNCC	6 cut(s)	176, 238, 250, 479, 2160, 2220
BspMI	ACCTGC	1 cut(s)	1502
BspPI	GGATC	4 cut(s)	231, 244, 1927, 2225
BspT104I	TTCGAA	2 cut(s)	281, 2327
BspT107I	GGYRCC	2 cut(s)	174, 2218
BspTI	CTTAAG	1 cut(s)	2447
BsrBI	CCGCTC	1 cut(s)	208
BsrDI	GCAATG	2 cut(s)	194, 1881
BsrI	ACTGG	8 cut(s)	323, 1166, 1219, 1338, 1578, 1893, 2122, 2160
BssECI	CCNNGG	9 cut(s)	178, 232, 509, 1197, 1965, 2162, 2208, 2268, 2458
BssMI	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	5 cut(s)	178, 1197, 2162, 2208, 2268
Bst2UI	CCWGG	7 cut(s)	241, 716, 811, 1193, 1479, 1966, 2460
Bst6I	CTCTTC	8 cut(s)	81, 278, 820, 861, 912, 1167, 1659, 1734
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	2447
BstAPI	GCANNNNNTGC	2 cut(s)	1517, 1757
BstBI	TTCGAA	2 cut(s)	281, 2327
BstC8I	GCNNGC	7 cut(s)	210, 676, 680, 1910, 2029, 2079, 2370
BstDEI	CTNAG	6 cut(s)	65, 170, 204, 306, 908, 1524
BstENI	CCTNNNNNAGG	1 cut(s)	1196
BstF5I	GGATG	7 cut(s)	519, 862, 1009, 1126, 1192, 1378, 1440
BstFNI	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
BstH2I	RGCGCY	1 cut(s)	72
BstHHI	GCGC	4 cut(s)	71, 112, 348, 2346
BstKTI	GATC	8 cut(s)	239, 563, 1081, 1495, 1567, 1705, 1935, 2233
BstMAI	GTCTC	3 cut(s)	18, 1096, 1736
BstMBI	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
BstMCI	CGRYCG	1 cut(s)	393
BstMWI	GCNNNNNNNGC	9 cut(s)	205, 275, 1343, 1355, 1517, 1757, 2303, 2350, 2365
BstNI	CCWGG	7 cut(s)	241, 716, 811, 1193, 1479, 1966, 2460
BstNSI	RCATGY	1 cut(s)	1751
BstSFI	CTRYAG	2 cut(s)	1143, 1350
BstSLI	GKGCMC	1 cut(s)	1214
BstUI	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
BstV1I	GCAGC	3 cut(s)	989, 1333, 2306
BstV2I	GAAGAC	3 cut(s)	148, 726, 1805
BstX2I	RGATCY	2 cut(s)	236, 1932
BstYI	RGATCY	2 cut(s)	236, 1932
BsuRI	GGCC	5 cut(s)	23, 363, 641, 1196, 1337
BtsCI	GGATG	7 cut(s)	519, 862, 1009, 1126, 1192, 1378, 1440
BtsI	GCAGTG	1 cut(s)	1741
BtsIMutI	CAGTG	4 cut(s)	1212, 1653, 1741, 2261
BveI	ACCTGC	1 cut(s)	1502
Cac8I	GCNNGC	7 cut(s)	210, 676, 680, 1910, 2029, 2079, 2370
CfoI	GCGC	4 cut(s)	71, 112, 348, 2346
Cfr13I	GGNCC	5 cut(s)	249, 361, 477, 640, 1669
CseI	GACGC	2 cut(s)	1645, 1774
Csp6I	GTAC	7 cut(s)	137, 175, 256, 1148, 1307, 1413, 2219
CviAII	CATG	4 cut(s)	31, 100, 1748, 2392
CviQI	GTAC	7 cut(s)	137, 175, 256, 1148, 1307, 1413, 2219
DdeI	CTNAG	6 cut(s)	65, 170, 204, 306, 908, 1524
DpnI	GATC	8 cut(s)	238, 562, 1080, 1494, 1566, 1704, 1934, 2232
DpnII	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
EaeI	YGGCCR	3 cut(s)	21, 1194, 1335
Eam1104I	CTCTTC	8 cut(s)	81, 278, 820, 861, 912, 1167, 1659, 1734
EarI	CTCTTC	8 cut(s)	81, 278, 820, 861, 912, 1167, 1659, 1734
EciI	GGCGGA	1 cut(s)	1639
Ecl136II	GAGCTC	1 cut(s)	310
Eco130I	CCWWGG	5 cut(s)	178, 1197, 2162, 2208, 2268
Eco24I	GRGCYC	1 cut(s)	312
Eco32I	GATATC	1 cut(s)	1597
Eco47I	GGWCC	3 cut(s)	249, 477, 1669
Eco53kI	GAGCTC	1 cut(s)	310
Eco57I	CTGAAG	3 cut(s)	885, 1683, 2208
Eco88I	CYCGRG	5 cut(s)	129, 222, 457, 1326, 2312
EcoICRI	GAGCTC	1 cut(s)	310
EcoNI	CCTNNNNNAGG	1 cut(s)	1196
EcoRI	GAATTC	2 cut(s)	595, 1979
EcoRII	CCWGG	7 cut(s)	239, 714, 809, 1191, 1477, 1964, 2458
EcoRV	GATATC	1 cut(s)	1597
EcoT14I	CCWWGG	5 cut(s)	178, 1197, 2162, 2208, 2268
EcoT38I	GRGCYC	1 cut(s)	312
ErhI	CCWWGG	5 cut(s)	178, 1197, 2162, 2208, 2268
Esp3I	CGTCTC	2 cut(s)	18, 1096
FaeI	CATG	4 cut(s)	34, 103, 1751, 2395
FalI	AAGNNNNNCTT	4 cut(s)	1146, 1178, 1901, 1933
FaqI	GGGAC	3 cut(s)	385, 463, 2441
FatI	CATG	4 cut(s)	30, 99, 1747, 2391
FauI	CCCGC	6 cut(s)	102, 144, 201, 262, 2024, 2419
FbaI	TGATCA	2 cut(s)	1078, 1564
Fnu4HI	GCNGC	5 cut(s)	84, 349, 978, 1347, 2295
FokI	GGATG	7 cut(s)	526, 869, 1016, 1133, 1199, 1365, 1427
FriOI	GRGCYC	1 cut(s)	312
Fsp4HI	GCNGC	5 cut(s)	84, 349, 978, 1347, 2295
FspBI	CTAG	5 cut(s)	179, 1203, 1427, 1941, 2057
GlaI	GCGC	4 cut(s)	70, 111, 347, 2345
GluI	GCNGC	5 cut(s)	84, 349, 978, 1347, 2295
GsuI	CTGGAG	1 cut(s)	462
HaeII	RGCGCY	1 cut(s)	72
HaeIII	GGCC	5 cut(s)	23, 363, 641, 1196, 1337
HapII	CCGG	8 cut(s)	80, 232, 252, 356, 509, 540, 638, 1984
HgaI	GACGC	2 cut(s)	1645, 1774
HhaI	GCGC	4 cut(s)	71, 112, 348, 2346
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	4 cut(s)	34, 103, 1751, 2395
Hin6I	GCGC	4 cut(s)	69, 110, 346, 2344
HinP1I	GCGC	4 cut(s)	69, 110, 346, 2344
HincII	GTYRAC	1 cut(s)	1843
HindII	GTYRAC	1 cut(s)	1843
HindIII	AAGCTT	1 cut(s)	2423
HpaII	CCGG	8 cut(s)	80, 232, 252, 356, 509, 540, 638, 1984
HphI	GGTGA	6 cut(s)	326, 464, 722, 802, 1257, 1267
Hpy166II	GTNNAC	1 cut(s)	1843
Hpy8I	GTNNAC	1 cut(s)	1843
Hpy99I	CGWCG	1 cut(s)	13
HpyAV	CCTTC	8 cut(s)	258, 456, 1392, 1476, 1724, 1910, 2232, 2242
HpyCH4IV	ACGT	2 cut(s)	11, 1839
HpyF10VI	GCNNNNNNNGC	9 cut(s)	205, 275, 1343, 1355, 1517, 1757, 2303, 2350, 2365
HpyF3I	CTNAG	6 cut(s)	65, 170, 204, 306, 908, 1524
HpySE526I	ACGT	2 cut(s)	11, 1839
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	4 cut(s)	34, 103, 1751, 2395
HspAI	GCGC	4 cut(s)	69, 110, 346, 2344
Kpn2I	TCCGGA	1 cut(s)	1983
KpnI	GGTACC	2 cut(s)	178, 2222
Ksp22I	TGATCA	2 cut(s)	1078, 1564
Kzo9I	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
LmnI	GCTCC	6 cut(s)	275, 420, 1052, 1783, 2164, 2365
Lsp1109I	GCAGC	3 cut(s)	989, 1333, 2306
LweI	GCATC	2 cut(s)	1111, 1449
MaeI	CTAG	5 cut(s)	179, 1203, 1427, 1941, 2057
MaeII	ACGT	2 cut(s)	11, 1839
MaeIII	GTNAC	7 cut(s)	314, 338, 434, 452, 1879, 2008, 2151
MalI	GATC	8 cut(s)	238, 562, 1080, 1494, 1566, 1704, 1934, 2232
MbiI	CCGCTC	1 cut(s)	208
MboI	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	2 cut(s)	236, 1932
MhlI	GDGCHC	3 cut(s)	312, 1214, 2263
MlsI	TGGCCA	2 cut(s)	1196, 1337
MluNI	TGGCCA	2 cut(s)	1196, 1337
MlyI	GAGTC	7 cut(s)	54, 295, 335, 410, 431, 449, 1046
MmeI	TCCRAC	2 cut(s)	82, 1074
Mox20I	TGGCCA	2 cut(s)	1196, 1337
MroI	TCCGGA	1 cut(s)	1983
MscI	TGGCCA	2 cut(s)	1196, 1337
MseI	TTAA	6 cut(s)	332, 962, 1818, 2190, 2280, 2448
MslI	CAYNNNNRTG	2 cut(s)	29, 2066
Msp20I	TGGCCA	2 cut(s)	1196, 1337
MspA1I	CMGCKG	3 cut(s)	1040, 1099, 1616
MspCI	CTTAAG	1 cut(s)	2447
MspI	CCGG	8 cut(s)	80, 232, 252, 356, 509, 540, 638, 1984
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	212
MvaI	CCWGG	7 cut(s)	241, 716, 811, 1193, 1479, 1966, 2460
MvnI	CGCG	6 cut(s)	110, 151, 346, 348, 2031, 2346
MwoI	GCNNNNNNNGC	9 cut(s)	205, 275, 1343, 1355, 1517, 1757, 2303, 2350, 2365
NciI	CCSGG	4 cut(s)	233, 253, 510, 541
NdeII	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
NlaIII	CATG	4 cut(s)	34, 103, 1751, 2395
NlaIV	GGNNCC	6 cut(s)	176, 238, 250, 479, 2160, 2220
NmeAIII	GCCGAG	1 cut(s)	2412
NmuCI	GTSAC	6 cut(s)	314, 338, 434, 452, 1879, 2008
NspI	RCATGY	1 cut(s)	1751
NspV	TTCGAA	2 cut(s)	281, 2327
PaeR7I	CTCGAG	1 cut(s)	457
PctI	GAATGC	1 cut(s)	212
PfeI	GAWTC	9 cut(s)	589, 617, 650, 752, 800, 1067, 1113, 1462, 1584
PflFI	GACNNNGTC	2 cut(s)	247, 320
PfoI	TCCNGGA	2 cut(s)	239, 539
PkrI	GCNGC	5 cut(s)	85, 350, 979, 1348, 2296
PleI	GAGTC	7 cut(s)	54, 294, 335, 409, 431, 449, 1046
PpsI	GAGTC	7 cut(s)	54, 294, 335, 409, 431, 449, 1046
Psp124BI	GAGCTC	1 cut(s)	312
Psp6I	CCWGG	7 cut(s)	239, 714, 809, 1191, 1477, 1964, 2458
PspGI	CCWGG	7 cut(s)	239, 714, 809, 1191, 1477, 1964, 2458
PspN4I	GGNNCC	6 cut(s)	176, 238, 250, 479, 2160, 2220
PspPI	GGNCC	5 cut(s)	249, 361, 477, 640, 1669
PsuI	RGATCY	2 cut(s)	236, 1932
PsyI	GACNNNGTC	2 cut(s)	247, 320
PvuII	CAGCTG	2 cut(s)	1040, 1616
RsaI	GTAC	7 cut(s)	138, 176, 257, 1149, 1308, 1414, 2220
RsaNI	GTAC	7 cut(s)	137, 175, 256, 1148, 1307, 1413, 2219
RseI	CAYNNNNRTG	2 cut(s)	29, 2066
SacI	GAGCTC	1 cut(s)	312
SaqAI	TTAA	6 cut(s)	332, 962, 1818, 2190, 2280, 2448
SatI	GCNGC	5 cut(s)	84, 349, 978, 1347, 2295
Sau3AI	GATC	8 cut(s)	236, 560, 1078, 1492, 1564, 1702, 1932, 2230
Sau96I	GGNCC	5 cut(s)	249, 361, 477, 640, 1669
SchI	GAGTC	7 cut(s)	54, 295, 335, 410, 431, 449, 1046
SduI	GDGCHC	3 cut(s)	312, 1214, 2263
SfaNI	GCATC	2 cut(s)	1111, 1449
SfcI	CTRYAG	2 cut(s)	1143, 1350
Sfr274I	CTCGAG	1 cut(s)	457
SfuI	TTCGAA	2 cut(s)	281, 2327
SinI	GGWCC	3 cut(s)	249, 477, 1669
SlaI	CTCGAG	1 cut(s)	457
SmiMI	CAYNNNNRTG	2 cut(s)	29, 2066
SmlI	CTYRAG	2 cut(s)	457, 2447
SmoI	CTYRAG	2 cut(s)	457, 2447
SspI	AATATT	2 cut(s)	2148, 2188
SspMI	CTAG	5 cut(s)	179, 1203, 1427, 1941, 2057
SstI	GAGCTC	1 cut(s)	312
StyI	CCWWGG	5 cut(s)	178, 1197, 2162, 2208, 2268
TaiI	ACGT	2 cut(s)	14, 1842
TaqII	GACCGA	2 cut(s)	407, 465
TatI	WGTACW	2 cut(s)	1147, 1306
TauI	GCSGC	2 cut(s)	86, 351
TfiI	GAWTC	9 cut(s)	589, 617, 650, 752, 800, 1067, 1113, 1462, 1584
Tru1I	TTAA	6 cut(s)	332, 962, 1818, 2190, 2280, 2448
Tru9I	TTAA	6 cut(s)	332, 962, 1818, 2190, 2280, 2448
TscAI	CASTG	4 cut(s)	1219, 1660, 1741, 2268
TseFI	GTSAC	6 cut(s)	314, 338, 434, 452, 1879, 2008
TseI	GCWGC	3 cut(s)	977, 1346, 2294
Tsp45I	GTSAC	6 cut(s)	314, 338, 434, 452, 1879, 2008
TspGWI	ACGGA	3 cut(s)	1078, 1577, 1661
TspRI	CASTG	4 cut(s)	1219, 1660, 1741, 2268
Tth111I	GACNNNGTC	2 cut(s)	247, 320
Vha464I	CTTAAG	1 cut(s)	2447
VpaK11BI	GGWCC	3 cut(s)	249, 477, 1669
XagI	CCTNNNNNAGG	1 cut(s)	1196
XapI	RAATTY	7 cut(s)	104, 595, 695, 757, 1261, 1979, 2378
XbaI	TCTAGA	2 cut(s)	1426, 1940
XceI	RCATGY	1 cut(s)	1751
XhoI	CTCGAG	1 cut(s)	457
XmaJI	CCTAGG	1 cut(s)	178
XspI	CTAG	5 cut(s)	179, 1203, 1427, 1941, 2057
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.