Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Reverse (-)

21675312 .. 21679844

Gene Structure 4533 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1148200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2502 bp

ATGAAAGCGACGTCGCTTTCGGCAACTTTCATGCCCGTCAGACTCTTACCCCAACTCCGACTCCTCAGTACCTCTGTTCCGGCGACCCGTTACCCGCCCATGAAATTCGCGCCTTCCATTTCGTCCTCTCAGGCCGTACCCATTTTGTCGCGGGTCTTCCCCTTGAGGCTTAGGTACGTAGGGTTAGGGCTTCGTCGCTCTCTGCAGACTGAGCGAGCATTTTCAAGTCGGGCGTTCCGGGGCTCCAGGACCGGGTCGGGGTCCGAGTTGCCACGTGCCTACGAGAGAAGAGCGGGTGGAGCTTCGAAGAGTCTGATTGAGGACGAGGCTGAGCTCAGTGACTGGGTCAGCGAGTTAAAGAGTGACTCGCGTGGCGAATCGGAGGGCCGTAGGGGGACGGTTGGAGATAGGGGTACCGATAGGCAGTCTTATCCGATGAAGAGGAGCAGGAGAGATGGTGATTCTGGAGATTTTGGTGATTCTAAAAGGCGAGAATTTCGATCACCGAATCAGACTTTTTCGAGAAATTCGGGGATGAGTAAGCGATTTGATAATAAATCTGAGGGTGATAGGAAAGAGAGAGCATTTCCACCCAGAAACAATCGTGGGAATCCGAATTCAAATGGCGATTTTGGAGATTTGAGTAGGCGAAGATTTCCGGCCCAAAATGATTCCTTTTCGAGAAATGCAGGGACAAGCAATCGCTTTGATAACAAATTTCGGAGTGATGATGACAAGGAAGACAGATTTCCCCATAGAAACAATCGTGGGGCCTCGAATTCGTTTTCAAAAGGGAGTTCGAGTTCAAAGGTGAAAGGGGAATCATTTACCAGGAATAGAGGTGGAAGAGATTCGGGTAAAGAATTGCGAGTAATGGATGATACTGAAGAGGAGGAGGTAAAACCATTTGGTGGTATTGGGGGTTTTCTTAGTGAGGAAGAGAGTGACACTGCTGCTTCTGATGAAGATGGTTTTGGAGTATTAAGAGAAAAAAGTGCAGCTTCTTTGCTTCGTGGTTCAGATGATGAAGCGAGCAAAAGGGTTCTGCCGAAAAGTTCAGCTGGAAGCTCTGACTCTTATTTGAGTGAATCGCGGTTTGATCAATGTTCGGTCTCTCCACTGTCCTTGAAAGGAATCAAGGACGCAGGATATGAAAAGATGACTGTAGTGCAAGAAGCTACTCTTCCAGTAATATTGAAAGGCAAGGATGTGCTGGCCAAGGCTAGAACAGGCACTGGAAAAACTGTGGCGTTCTTGCTTCCATCAATTGAAGTTGTTGTAAATTCGCCTCCTATTACCCGTGATAACAAGCGACCACCAATTTTTGTACTTGTTATATGCCCAACCAGGGAGTTGGCAAGTCAAGCTGCTACTGAAGCCAATAAGTTGTTGAAATATCATCCCTCTATTGGTGTCCAAGTTGTGATTGGTGGTACACGACTTGCTTTAGAACAGAAACGTATCCAAGCCAACCCTTGCCAGATTCTTGTCGCTACACCTGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTTGCAACTAGGCTGATGGGTGTCAAAGTCCTTGTACTTGATGAAGCTGATCATTTACTGGATATGGGATTCCGTAAAGATATTGAAAGGATTATATCAGCTGTTCCCAAACAACGGCAGACACTTTTGTTTTCCGCCACTGTTCCGGAAGAGGTCCGTCAAATCTGTCATATTGCTTTGAAAAGAGATCACGAATATATCAATACAGTTGAAGAAGGCAGTGAAGAGACACATGCACAGGTCGCACAGACGCATTTGGTCGCTCCATTGGACAGGCATTTTTCCTTGGTATATAGTCTTCTTAAAGAGCATATTGCAGATGATGTTGACTATAAGGTTCTTGTATTCTGCACTACCGCTATGGTAACACGACTGGTTGCAGACCTTCTTGGTGAGCTGAACTTGAATGTCAGAGAGATCCATTCTAGAAAGCCACAGAGTTATAGAACCAGGGTTTCTGATGAATTTCGGAAATCAAAGGGTCTTATTCTTGTGACTTCTGATGTATCTGCACGTGGGGTTGATTATCCAGATGTTTCCCTAGTCATACAGGTAGTGGGCGTGCCAGCCGATAGACAACAGTATATACATCGACTTGGAAGAACTGGTCGTAAAGGCAAAGTGGGGCAAGGGATATTGCTACTGGCTCCTTGGGAGGAGTTCTTCTTGTCCAATATTAAGGATTTGCCAATATCCAAGGCTGCTGCACCGTCAGTCGATCCAGATACTACGAAGAAGGTGGAACGAGCACTGTCCAAGGTGGAGATGAAGAACAAAGAAGCAGCGTACCAAGCATGGCTGGGGTATTACAATTCAAACAAGAAAGTGGGGCGCGATAAGCATATGCTTGTGGAGCTTGCTAATGAGTTTAGCCGAAGCATGGGGCTGGACAACCCTCCGGCCATTCCCAAGCTCGTTCTTGGCAAGATGGGGCTTAAGAATGTCCCTGGATTGCGTTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

834

Amino Acids

92.28

Weight (kDa)

9.66

Isoelectric Point (pI)

51.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	389 - 566	1.3e-45	DEAD/DEAH box helicase
ResIII	PF04851	400 - 561	6.5e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	612 - 718	9.6e-20	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc36I	ACCTGC	1 cut(s)	1523
Acc65I	GGTACC	1 cut(s)	413
AccB1I	GGYRCC	1 cut(s)	413
AccB7I	CCANNNNNTGG	1 cut(s)	911
AccBSI	CCGCTC	1 cut(s)	293
AccII	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
AccIII	TCCGGA	1 cut(s)	1681
AciI	CCGC	7 cut(s)	95, 151, 293, 1093, 1530, 1671, 1893
AclWI	GGATC	2 cut(s)	1948, 2249
AcoI	YGGCCR	2 cut(s)	1215, 2436
AcsI	RAATTY	8 cut(s)	104, 494, 526, 616, 716, 778, 1282, 2000
AcuI	CTGAAG	2 cut(s)	906, 1395
AcvI	CACGTG	2 cut(s)	275, 2051
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	8 cut(s)	70, 138, 176, 415, 1329, 1435, 1572, 2324
AfiI	CCNNNNNNNGG	7 cut(s)	252, 258, 911, 1348, 1409, 1650, 2416
AflII	CTTAAG	1 cut(s)	2471
AjnI	CCWGG	6 cut(s)	245, 830, 1346, 1498, 1985, 2482
AjuI	GAANNNNNNNTTGG	2 cut(s)	957, 989
AloI	GAACNNNNNNTCC	2 cut(s)	787, 819
Alw21I	GWGCWC	2 cut(s)	336, 2287
Alw26I	GTCTC	2 cut(s)	1117, 1757
AlwI	GGATC	2 cut(s)	1948, 2249
AlwNI	CAGNNNCTG	2 cut(s)	342, 1235
Aor13HI	TCCGGA	1 cut(s)	1681
AoxI	GGCC	6 cut(s)	132, 385, 660, 771, 1215, 2436
ApeKI	GCWGC	6 cut(s)	953, 998, 1367, 2237, 2240, 2318
ApoI	RAATTY	8 cut(s)	104, 494, 526, 616, 716, 778, 1282, 2000
ArsI	GACNNNNNNTTYG	1 cut(s)	33
Asp700I	GAANNNNTTC	1 cut(s)	850
Asp718I	GGTACC	1 cut(s)	413
AspLEI	GCGC	2 cut(s)	112, 2370
AspS9I	GGNCC	6 cut(s)	249, 261, 385, 661, 771, 1690
AsuC2I	CCSGG	2 cut(s)	239, 253
AsuHPI	GGTGA	6 cut(s)	470, 488, 495, 578, 823, 1940
AsuII	TTCGAA	1 cut(s)	305
AvaII	GGWCC	3 cut(s)	249, 261, 1690
BalI	TGGCCA	1 cut(s)	1217
BanI	GGYRCC	1 cut(s)	413
BanII	GRGCYC	2 cut(s)	245, 336
BarI	GAAGNNNNNNTAC	2 cut(s)	1818, 1850
BbrPI	CACGTG	2 cut(s)	275, 2051
BbsI	GAAGAC	3 cut(s)	148, 747, 1826
Bbv12I	GWGCWC	2 cut(s)	336, 2287
BbvI	GCAGC	6 cut(s)	940, 1010, 1354, 2224, 2227, 2330
BccI	CCATC	5 cut(s)	449, 962, 1270, 1546, 2458
BceAI	ACGGC	3 cut(s)	119, 372, 1667
BciT130I	CCWGG	6 cut(s)	247, 832, 1348, 1500, 1987, 2484
BciVI	GTATCC	1 cut(s)	1472
BclI	TGATCA	2 cut(s)	1099, 1585
BcnI	CCSGG	2 cut(s)	239, 253
BcoDI	GTCTC	2 cut(s)	1117, 1757
BfaI	CTAG	4 cut(s)	1224, 1545, 1962, 2078
BfmI	CTRYAG	2 cut(s)	203, 1164
BfrI	CTTAAG	1 cut(s)	2471
BfuAI	ACCTGC	1 cut(s)	1523
BfuI	GTATCC	1 cut(s)	1472
BisI	GCNGC	6 cut(s)	954, 999, 1368, 2238, 2241, 2319
BlpI	GCTNAGC	1 cut(s)	330
BlsI	GCNGC	6 cut(s)	955, 1000, 1369, 2239, 2242, 2320
Bme1390I	CCNGG	8 cut(s)	239, 247, 253, 832, 1348, 1500, 1987, 2484
Bme18I	GGWCC	3 cut(s)	249, 261, 1690
BmgT120I	GGNCC	6 cut(s)	249, 261, 385, 661, 771, 1690
BmiI	GGNNCC	5 cut(s)	244, 262, 415, 772, 2184
BmrFI	CCNGG	8 cut(s)	239, 247, 253, 832, 1348, 1500, 1987, 2484
BmrI	ACTGGG	1 cut(s)	352
BmuI	ACTGGG	1 cut(s)	352
BpiI	GAAGAC	3 cut(s)	148, 747, 1826
BpmI	CTGGAG	2 cut(s)	229, 486
Bpu10I	CCTNAGC	1 cut(s)	170
Bpu1102I	GCTNAGC	1 cut(s)	330
Bpu14I	TTCGAA	1 cut(s)	305
BpuEI	CTTGAG	1 cut(s)	184
BpuMI	CCSGG	2 cut(s)	239, 253
BsaAI	YACGTR	3 cut(s)	178, 275, 2051
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	1 cut(s)	1117
BsaJI	CCNNGG	9 cut(s)	238, 1218, 1347, 1821, 1986, 2186, 2232, 2292, 2482
BsaWI	WCCGGW	1 cut(s)	1681
BsaXI	ACNNNNNCTCC	8 cut(s)	39, 45, 69, 75, 442, 472, 787, 817
Bsc4I	CCNNNNNNNGG	7 cut(s)	252, 258, 911, 1348, 1409, 1650, 2416
Bse1I	ACTGG	7 cut(s)	347, 1187, 1240, 1599, 1914, 2146, 2184
BseAI	TCCGGA	1 cut(s)	1681
BseBI	CCWGG	6 cut(s)	247, 832, 1348, 1500, 1987, 2484
BseDI	CCNNGG	9 cut(s)	238, 1218, 1347, 1821, 1986, 2186, 2232, 2292, 2482
BseGI	GGATG	4 cut(s)	540, 883, 1213, 1399
BseLI	CCNNNNNNNGG	7 cut(s)	252, 258, 911, 1348, 1409, 1650, 2416
BseMII	CTCAG	6 cut(s)	79, 143, 201, 321, 349, 552
BseNI	ACTGG	7 cut(s)	347, 1187, 1240, 1599, 1914, 2146, 2184
BseRI	GAGGAG	5 cut(s)	53, 457, 905, 908, 2207
BseXI	GCAGC	6 cut(s)	940, 1010, 1354, 2224, 2227, 2330
BseYI	CCCAGC	1 cut(s)	2335
BsgI	GTGCAG	4 cut(s)	1017, 1870, 2031, 2226
Bsh1236I	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
BshFI	GGCC	6 cut(s)	134, 387, 662, 773, 1217, 2438
BshNI	GGYRCC	1 cut(s)	413
BsiHKAI	GWGCWC	2 cut(s)	336, 2287
BsiSI	CCGG	6 cut(s)	80, 238, 252, 659, 1682, 2435
BslFI	GGGAC	3 cut(s)	409, 706, 2465
BslI	CCNNNNNNNGG	7 cut(s)	252, 258, 911, 1348, 1409, 1650, 2416
BsmAI	GTCTC	2 cut(s)	1117, 1757
BsmFI	GGGAC	3 cut(s)	409, 706, 2465
BsnI	GGCC	6 cut(s)	134, 387, 662, 773, 1217, 2438
Bso31I	GGTCTC	1 cut(s)	1117
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	3 cut(s)	245, 336, 2287
Bsp13I	TCCGGA	1 cut(s)	1681
Bsp143I	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
Bsp1720I	GCTNAGC	1 cut(s)	330
BspACI	CCGC	7 cut(s)	95, 151, 293, 1093, 1530, 1671, 1893
BspANI	GGCC	6 cut(s)	134, 387, 662, 773, 1217, 2438
BspCNI	CTCAG	6 cut(s)	78, 142, 202, 322, 348, 553
BspEI	TCCGGA	1 cut(s)	1681
BspFNI	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
BspLI	GGNNCC	5 cut(s)	244, 262, 415, 772, 2184
BspMAI	CTGCAG	1 cut(s)	207
BspMI	ACCTGC	1 cut(s)	1523
BspPI	GGATC	2 cut(s)	1948, 2249
BspQI	GCTCTTC	1 cut(s)	283
BspT104I	TTCGAA	1 cut(s)	305
BspT107I	GGYRCC	1 cut(s)	413
BspTI	CTTAAG	1 cut(s)	2471
BspTNI	GGTCTC	1 cut(s)	1117
BsrBI	CCGCTC	1 cut(s)	293
BsrI	ACTGG	7 cut(s)	347, 1187, 1240, 1599, 1914, 2146, 2184
BssECI	CCNNGG	9 cut(s)	238, 1218, 1347, 1821, 1986, 2186, 2232, 2292, 2482
BssMI	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	5 cut(s)	1218, 1821, 2186, 2232, 2292
Bst2UI	CCWGG	6 cut(s)	247, 832, 1348, 1500, 1987, 2484
Bst4CI	ACNGT	9 cut(s)	400, 1122, 1165, 1246, 1678, 1744, 2118, 2247, 2289
Bst6I	CTCTTC	9 cut(s)	283, 302, 434, 841, 882, 933, 1188, 1680, 1755
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	2471
BstAPI	GCANNNNNTGC	1 cut(s)	1538
BstBAI	YACGTR	3 cut(s)	178, 275, 2051
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	6 cut(s)	216, 1033, 1215, 2099, 2103, 2394
BstDEI	CTNAG	8 cut(s)	65, 129, 170, 210, 330, 335, 561, 929
BstF5I	GGATG	4 cut(s)	540, 883, 1213, 1399
BstFNI	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
BstHHI	GCGC	2 cut(s)	112, 2370
BstKTI	GATC	7 cut(s)	503, 1102, 1516, 1588, 1726, 1956, 2257
BstMAI	GTCTC	2 cut(s)	1117, 1757
BstMBI	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
BstMWI	GCNNNNNNNGC	9 cut(s)	211, 299, 1364, 1376, 1538, 1778, 2327, 2374, 2389
BstNI	CCWGG	6 cut(s)	247, 832, 1348, 1500, 1987, 2484
BstNSI	RCATGY	1 cut(s)	1772
BstSCI	CCNGG	8 cut(s)	237, 245, 251, 830, 1346, 1498, 1985, 2482
BstSFI	CTRYAG	2 cut(s)	203, 1164
BstSNI	TACGTA	1 cut(s)	178
BstUI	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
BstV1I	GCAGC	6 cut(s)	940, 1010, 1354, 2224, 2227, 2330
BstV2I	GAAGAC	3 cut(s)	148, 747, 1826
BstX2I	RGATCY	1 cut(s)	1953
BstXI	CCANNNNNNTGG	1 cut(s)	1354
BstYI	RGATCY	1 cut(s)	1953
BsuI	GTATCC	1 cut(s)	1472
BsuRI	GGCC	6 cut(s)	134, 387, 662, 773, 1217, 2438
BtsCI	GGATG	4 cut(s)	540, 883, 1213, 1399
BtsI	GCAGTG	2 cut(s)	948, 1762
BtsIMutI	CAGTG	7 cut(s)	343, 948, 1118, 1233, 1674, 1762, 2285
BveI	ACCTGC	1 cut(s)	1523
Cac8I	GCNNGC	6 cut(s)	216, 1033, 1215, 2099, 2103, 2394
CaiI	CAGNNNCTG	2 cut(s)	342, 1235
CfoI	GCGC	2 cut(s)	112, 2370
Cfr13I	GGNCC	6 cut(s)	249, 261, 385, 661, 771, 1690
CseI	GACGC	2 cut(s)	1151, 1795
Csp6I	GTAC	8 cut(s)	69, 137, 175, 414, 1328, 1434, 1571, 2323
CspCI	CAANNNNNGTGG	2 cut(s)	1107, 1142
CviAII	CATG	5 cut(s)	31, 100, 1769, 2331, 2416
CviQI	GTAC	8 cut(s)	69, 137, 175, 414, 1328, 1434, 1571, 2323
DdeI	CTNAG	8 cut(s)	65, 129, 170, 210, 330, 335, 561, 929
DpnI	GATC	7 cut(s)	502, 1101, 1515, 1587, 1725, 1955, 2256
DpnII	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
EaeI	YGGCCR	2 cut(s)	1215, 2436
Eam1104I	CTCTTC	9 cut(s)	283, 302, 434, 841, 882, 933, 1188, 1680, 1755
EarI	CTCTTC	9 cut(s)	283, 302, 434, 841, 882, 933, 1188, 1680, 1755
EciI	GGCGGA	1 cut(s)	1660
Ecl136II	GAGCTC	1 cut(s)	334
Eco105I	TACGTA	1 cut(s)	178
Eco130I	CCWWGG	5 cut(s)	1218, 1821, 2186, 2232, 2292
Eco24I	GRGCYC	2 cut(s)	245, 336
Eco31I	GGTCTC	1 cut(s)	1117
Eco47I	GGWCC	3 cut(s)	249, 261, 1690
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	2 cut(s)	906, 1395
Eco72I	CACGTG	2 cut(s)	275, 2051
EcoICRI	GAGCTC	1 cut(s)	334
EcoO109I	RGGNCCY	1 cut(s)	771
EcoRI	GAATTC	2 cut(s)	616, 778
EcoRII	CCWGG	6 cut(s)	245, 830, 1346, 1498, 1985, 2482
EcoT14I	CCWWGG	5 cut(s)	1218, 1821, 2186, 2232, 2292
EcoT38I	GRGCYC	2 cut(s)	245, 336
ErhI	CCWWGG	5 cut(s)	1218, 1821, 2186, 2232, 2292
FaeI	CATG	5 cut(s)	34, 103, 1772, 2334, 2419
FalI	AAGNNNNNCTT	4 cut(s)	985, 1017, 1167, 1199
FaqI	GGGAC	3 cut(s)	409, 706, 2465
FatI	CATG	5 cut(s)	30, 99, 1768, 2330, 2415
FauI	CCCGC	3 cut(s)	102, 144, 286
FauNDI	CATATG	1 cut(s)	2379
FbaI	TGATCA	2 cut(s)	1099, 1585
Fnu4HI	GCNGC	6 cut(s)	954, 999, 1368, 2238, 2241, 2319
FokI	GGATG	4 cut(s)	547, 890, 1220, 1386
FriOI	GRGCYC	2 cut(s)	245, 336
Fsp4HI	GCNGC	6 cut(s)	954, 999, 1368, 2238, 2241, 2319
FspBI	CTAG	4 cut(s)	1224, 1545, 1962, 2078
GlaI	GCGC	2 cut(s)	111, 2369
GluI	GCNGC	6 cut(s)	954, 999, 1368, 2238, 2241, 2319
GsaI	CCCAGC	1 cut(s)	2339
GsuI	CTGGAG	2 cut(s)	229, 486
HaeIII	GGCC	6 cut(s)	134, 387, 662, 773, 1217, 2438
HapII	CCGG	6 cut(s)	80, 238, 252, 659, 1682, 2435
HgaI	GACGC	2 cut(s)	1151, 1795
HhaI	GCGC	2 cut(s)	112, 2370
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	5 cut(s)	34, 103, 1772, 2334, 2419
Hin6I	GCGC	2 cut(s)	110, 2368
HinP1I	GCGC	2 cut(s)	110, 2368
HincII	GTYRAC	1 cut(s)	1864
HindII	GTYRAC	1 cut(s)	1864
HpaII	CCGG	6 cut(s)	80, 238, 252, 659, 1682, 2435
HphI	GGTGA	6 cut(s)	470, 488, 495, 578, 823, 1940
Hpy166II	GTNNAC	2 cut(s)	1436, 1864
Hpy188III	TCNNGA	8 cut(s)	465, 522, 681, 1682, 1727, 1962, 2066, 2258
Hpy8I	GTNNAC	2 cut(s)	1436, 1864
Hpy99I	CGWCG	3 cut(s)	13, 16, 198
HpyAV	CCTTC	5 cut(s)	123, 1497, 1745, 1931, 2266
HpyCH4III	ACNGT	9 cut(s)	400, 1122, 1165, 1246, 1678, 1744, 2118, 2247, 2289
HpyCH4IV	ACGT	5 cut(s)	11, 177, 274, 1459, 2050
HpyF10VI	GCNNNNNNNGC	9 cut(s)	211, 299, 1364, 1376, 1538, 1778, 2327, 2374, 2389
HpyF3I	CTNAG	8 cut(s)	65, 129, 170, 210, 330, 335, 561, 929
HpySE526I	ACGT	5 cut(s)	11, 177, 274, 1459, 2050
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	5 cut(s)	34, 103, 1772, 2334, 2419
HspAI	GCGC	2 cut(s)	110, 2368
Kpn2I	TCCGGA	1 cut(s)	1681
KpnI	GGTACC	1 cut(s)	417
Ksp22I	TGATCA	2 cut(s)	1099, 1585
Kzo9I	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
LguI	GCTCTTC	1 cut(s)	283
LmnI	GCTCC	6 cut(s)	248, 299, 444, 1804, 2188, 2389
Lsp1109I	GCAGC	6 cut(s)	940, 1010, 1354, 2224, 2227, 2330
MaeI	CTAG	4 cut(s)	1224, 1545, 1962, 2078
MaeII	ACGT	5 cut(s)	11, 177, 274, 1459, 2050
MaeIII	GTNAC	6 cut(s)	89, 338, 362, 944, 1900, 2029
MalI	GATC	7 cut(s)	502, 1101, 1515, 1587, 1725, 1955, 2256
MbiI	CCGCTC	1 cut(s)	293
MboI	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
MfeI	CAATTG	1 cut(s)	1266
MflI	RGATCY	1 cut(s)	1953
MhlI	GDGCHC	3 cut(s)	245, 336, 2287
MlsI	TGGCCA	1 cut(s)	1217
MluNI	TGGCCA	1 cut(s)	1217
MlyI	GAGTC	5 cut(s)	36, 54, 319, 359, 1067
MmeI	TCCRAC	2 cut(s)	82, 382
Mox20I	TGGCCA	1 cut(s)	1217
MroI	TCCGGA	1 cut(s)	1681
MroXI	GAANNNNTTC	1 cut(s)	850
MscI	TGGCCA	1 cut(s)	1217
MseI	TTAA	5 cut(s)	356, 983, 1839, 2214, 2472
Msp20I	TGGCCA	1 cut(s)	1217
MspA1I	CMGCKG	2 cut(s)	1061, 1637
MspCI	CTTAAG	1 cut(s)	2471
MspI	CCGG	6 cut(s)	80, 238, 252, 659, 1682, 2435
MspR9I	CCNGG	8 cut(s)	239, 247, 253, 832, 1348, 1500, 1987, 2484
MunI	CAATTG	1 cut(s)	1266
MvaI	CCWGG	6 cut(s)	247, 832, 1348, 1500, 1987, 2484
MvnI	CGCG	5 cut(s)	110, 151, 370, 1093, 2370
MwoI	GCNNNNNNNGC	9 cut(s)	211, 299, 1364, 1376, 1538, 1778, 2327, 2374, 2389
NciI	CCSGG	2 cut(s)	239, 253
NdeI	CATATG	1 cut(s)	2379
NdeII	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
NlaIII	CATG	5 cut(s)	34, 103, 1772, 2334, 2419
NlaIV	GGNNCC	5 cut(s)	244, 262, 415, 772, 2184
NmuCI	GTSAC	4 cut(s)	338, 362, 944, 2029
NspI	RCATGY	1 cut(s)	1772
NspV	TTCGAA	1 cut(s)	305
PciSI	GCTCTTC	1 cut(s)	283
PdmI	GAANNNNTTC	1 cut(s)	850
PflFI	GACNNNGTC	2 cut(s)	253, 344
PflMI	CCANNNNNTGG	1 cut(s)	911
PfoI	TCCNGGA	1 cut(s)	245
PkrI	GCNGC	6 cut(s)	955, 1000, 1369, 2239, 2242, 2320
PleI	GAGTC	5 cut(s)	36, 54, 318, 359, 1067
PmaCI	CACGTG	2 cut(s)	275, 2051
PmlI	CACGTG	2 cut(s)	275, 2051
PpsI	GAGTC	5 cut(s)	36, 54, 318, 359, 1067
Ppu21I	YACGTR	3 cut(s)	178, 275, 2051
Psp124BI	GAGCTC	1 cut(s)	336
Psp6I	CCWGG	6 cut(s)	245, 830, 1346, 1498, 1985, 2482
PspCI	CACGTG	2 cut(s)	275, 2051
PspFI	CCCAGC	1 cut(s)	2335
PspGI	CCWGG	6 cut(s)	245, 830, 1346, 1498, 1985, 2482
PspN4I	GGNNCC	5 cut(s)	244, 262, 415, 772, 2184
PspPI	GGNCC	6 cut(s)	249, 261, 385, 661, 771, 1690
PstI	CTGCAG	1 cut(s)	207
PstNI	CAGNNNCTG	2 cut(s)	342, 1235
PsuI	RGATCY	1 cut(s)	1953
PsyI	GACNNNGTC	2 cut(s)	253, 344
PvuII	CAGCTG	2 cut(s)	1061, 1637
RsaI	GTAC	8 cut(s)	70, 138, 176, 415, 1329, 1435, 1572, 2324
RsaNI	GTAC	8 cut(s)	69, 137, 175, 414, 1328, 1434, 1571, 2323
SacI	GAGCTC	1 cut(s)	336
SapI	GCTCTTC	1 cut(s)	283
SaqAI	TTAA	5 cut(s)	356, 983, 1839, 2214, 2472
SatI	GCNGC	6 cut(s)	954, 999, 1368, 2238, 2241, 2319
Sau3AI	GATC	7 cut(s)	500, 1099, 1513, 1585, 1723, 1953, 2254
Sau96I	GGNCC	6 cut(s)	249, 261, 385, 661, 771, 1690
SchI	GAGTC	5 cut(s)	36, 54, 319, 359, 1067
ScrFI	CCNGG	8 cut(s)	239, 247, 253, 832, 1348, 1500, 1987, 2484
SduI	GDGCHC	3 cut(s)	245, 336, 2287
SfcI	CTRYAG	2 cut(s)	203, 1164
SfuI	TTCGAA	1 cut(s)	305
SinI	GGWCC	3 cut(s)	249, 261, 1690
SmlI	CTYRAG	2 cut(s)	163, 2471
SmoI	CTYRAG	2 cut(s)	163, 2471
SnaBI	TACGTA	1 cut(s)	178
SsiI	CCGC	7 cut(s)	95, 151, 293, 1093, 1530, 1671, 1893
SspI	AATATT	2 cut(s)	1194, 2212
SspMI	CTAG	4 cut(s)	1224, 1545, 1962, 2078
SstI	GAGCTC	1 cut(s)	336
StyD4I	CCNGG	8 cut(s)	237, 245, 251, 830, 1346, 1498, 1985, 2482
StyI	CCWWGG	5 cut(s)	1218, 1821, 2186, 2232, 2292
TaaI	ACNGT	9 cut(s)	400, 1122, 1165, 1246, 1678, 1744, 2118, 2247, 2289
TaiI	ACGT	5 cut(s)	14, 180, 277, 1462, 2053
TaqI	TCGA	8 cut(s)	305, 499, 521, 680, 776, 800, 2128, 2253
TaqII	GACCGA	1 cut(s)	1099
TatI	WGTACW	2 cut(s)	1327, 1570
Tru1I	TTAA	5 cut(s)	356, 983, 1839, 2214, 2472
Tru9I	TTAA	5 cut(s)	356, 983, 1839, 2214, 2472
TscAI	CASTG	7 cut(s)	343, 955, 1125, 1240, 1681, 1762, 2292
TseFI	GTSAC	4 cut(s)	338, 362, 944, 2029
TseI	GCWGC	6 cut(s)	953, 998, 1367, 2237, 2240, 2318
Tsp45I	GTSAC	4 cut(s)	338, 362, 944, 2029
TspGWI	ACGGA	2 cut(s)	1598, 1682
TspRI	CASTG	7 cut(s)	343, 955, 1125, 1240, 1681, 1762, 2292
Tth111I	GACNNNGTC	2 cut(s)	253, 344
Van91I	CCANNNNNTGG	1 cut(s)	911
Vha464I	CTTAAG	1 cut(s)	2471
VpaK11BI	GGWCC	3 cut(s)	249, 261, 1690
XapI	RAATTY	8 cut(s)	104, 494, 526, 616, 716, 778, 1282, 2000
XbaI	TCTAGA	1 cut(s)	1961
XceI	RCATGY	1 cut(s)	1772
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1424
XmnI	GAANNNNTTC	1 cut(s)	850
XspI	CTAG	4 cut(s)	1224, 1545, 1962, 2078
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.