Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Reverse (-)

51217834 .. 51223949

Gene Structure 6116 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG283200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2115 bp

ATGAAAACGACGTCGCTTTCGTCCACTATTATGCCCGTTAAGCTCCTCCTCAACAACCCTTCTCTCCCAGTAACCCGCTTCCTCTCCATGAAATTCGGACCCTCCATTTCCGCTCCTCGACCCGTACCCGCTTTCTCCCGGGTCTTCCCGCTCCGCCTCAGGTACCTCGCCCTCGGCTCCCAGCGCACATTCTCCACCCGCCGAACCGGGTCGGGCTCCCAATTCTCGCGCGGCTACGAGCGAAAGGCGGCGGTCGGGGGATCCAAGAGCCTGGTCGACGACGAGGCGGAGCTGAGTGACTGGGTCAGCGAGTTGAGGACCGACCCGACCCGCGGTGGCGGTGGCCGGGGAGAGGAATCGGACGGTGGTGGTCGGAGAGGTAGTAGGGTTAGAGATAGGGGCGGCGATAGAGAGCCGTATCCGGCGAAGCGGAGCAGGAGAGAAAGCGGCGCCGACGAGTTTGGTGAGTCGAGAACTCGGGGAGGGTTTCGGTCTTTTCCGGGAAGTTCACGGATGAGTAAAAGGGATGATCATGATGATGATAGAGAAGGTAGGTCATTTCCGCCGAGAAGCAATCGGGGGAGTTCGAGCTCGAATGACCGGTTTGGTGAGTCGAATCGGAGACGATTTCCGAGCTCAAATCCTTCGGCATTTACTAGAAATCCACGGATGGATAGAAGGTTTGATAACAAATTTGGGGACAATGCTGTGAAGGAAGATGGGTTTCCGGGGAGGAGAGGTCCAAATTTCAGAGGGAATTCGAATGCCAGTTCGAGTTCTTTGACTAAGAGAGGAGGGAGAGAGGTGGATTCGGGTTTCAGACGAGGTGGCGAGGGGTTGAGGAAGGGTGGGAGAGATTCGAGGAAGGAAAAGGAAATGCTTGTGGTGGATGATAGTGATGAGGATGAGGAGGTGAATGAGCTTGGTGTTGGCATTGGGGATTTGCTTACCGAGGAAGATAGTGACAGTGCTGCTTCGGATGAGGATGATGAGTACGAAGGATTGAAAGATAAGAGTTCAGCTGCATTGTTTGGTTCGGATAAGGAAGTGGTGAGTCAGAAGGCTGTGCCGAAAAGTTCAGCGGGAGACTCTGACTCTTATTTGAGCGAGTCAAGGTTTGATCAGTGTTCGGTCTCGCCACTGTCCTTGAAGGGAATCAAGGATGCGGGATATGAGAACATGACTGTGGTACAAGAGGCTACTCTTCCCATCATACTCAAAGGCAAGGATGTTCTAGCCAAGGCTAAAACCGGAACTGGAAAAACTGTTGCATTCTTGCTTCCATCAATTGAAGTTGTTGTAAAATCACCTCCTATTGGACGCGATCATAAGAGACCCCCAATTTCTGTACTTGTAATATGCCCAACCCGAGAGCTGGCAAGTCAAGCTGCGGCAGAAGCCAATAAATTGTTGAAGTATCATCCTTCTATTGGTGTTCAAGTTGTGATAGGAGGTACAAGACTTGCTCTAGAACAGAAACGCATGCAAGCAAACCCTTGCCAGATTCTTGTCGCTACACCGGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTTGCAACTAGGCTGATGGGTGTCAAAGTCCTCATTCTTGATGAAGCTGATCATCTACTGGATATGGGGTTCCGTAAAGACATTGAAAGGATTATAGCAGCTGTTCCAAAACAGCGACAGACACTACTGTTCTCTGCCACCGTTCCAGAAGAGGTCCGTCAAATCTGTCATATCGCTTTGAAAAGAGATCATGAATTTATTAACACGGTTCAGGAAGGCAGTGAAGAGACACATGCGCAGGTCAGACAAACCCATTTGGTTGCTCCTTTGGACAAACACTTTTCCTTTGTATATAGTCTTCTCAAAGAGCACATTGCTGACGATCCTGACTATAAGGTTCTCGTATTCTGCACTACTGCAATGGTCACACGACTGGTTGCTGACCTTCTTGGTGAGCTGAACTTGAATGTTAGAGAAATCCACTCCAGAAAGCCACAGAGTTATAGAACTAGGGTTTCCGATGAATTTCGGAAATCAAAGGGTCTTATCCTTGTGACTTCTGATGTATCTGCACGAGGGGTTGATTATCCAGACGTTACTCTAGTCATACAGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

704

Amino Acids

77.52

Weight (kDa)

9.18

Isoelectric Point (pI)

50.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	396 - 573	1.6e-45	DEAD/DEAH box helicase
ResIII	PF04851	407 - 568	4.4e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	619 - 703	3.8e-13	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	1794
Acc36I	ACCTGC	2 cut(s)	1544, 1786
Acc65I	GGTACC	1 cut(s)	162
AccB1I	GGYRCC	2 cut(s)	162, 449
AccBSI	CCGCTC	2 cut(s)	113, 151
AccI	GTMKAC	1 cut(s)	276
AccII	CGCG	4 cut(s)	229, 231, 333, 1323
AclWI	GGATC	3 cut(s)	255, 268, 1874
AcoI	YGGCCR	1 cut(s)	343
AcsI	RAATTY	6 cut(s)	92, 692, 745, 757, 1751, 2021
AcyI	GRCGYC	2 cut(s)	11, 450
AfaI	GTAC	6 cut(s)	126, 164, 995, 1191, 1350, 1456
AfiI	CCNNNNNNNGG	5 cut(s)	332, 352, 1316, 1375, 1430
AgeI	ACCGGT	1 cut(s)	600
AgsI	TTSAA	8 cut(s)	1006, 1150, 1292, 1414, 1439, 1643, 1738, 1963
AjnI	CCWGG	1 cut(s)	270
AloI	GAACNNNNNNTCC	2 cut(s)	1610, 1642
Alw21I	GWGCWC	3 cut(s)	593, 638, 1869
Alw26I	GTCTC	5 cut(s)	616, 1080, 1138, 1327, 1778
AlwI	GGATC	3 cut(s)	255, 268, 1874
Ama87I	CYCGRG	3 cut(s)	138, 477, 1368
AoxI	GGCC	1 cut(s)	343
ApeKI	GCWGC	4 cut(s)	971, 1022, 1388, 1655
ApoI	RAATTY	6 cut(s)	92, 692, 745, 757, 1751, 2021
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AsiGI	ACCGGT	1 cut(s)	600
Asp718I	GGTACC	1 cut(s)	162
AspLEI	GCGC	4 cut(s)	186, 231, 452, 1795
AspS9I	GGNCC	4 cut(s)	98, 318, 740, 1711
AsuC2I	CCSGG	7 cut(s)	139, 140, 208, 347, 501, 729, 1521
AsuHPI	GGTGA	6 cut(s)	476, 620, 925, 1063, 1299, 1961
AsuII	TTCGAA	1 cut(s)	761
AvaI	CYCGRG	3 cut(s)	138, 477, 1368
AvaII	GGWCC	4 cut(s)	98, 318, 740, 1711
AxyI	CCTNAGG	1 cut(s)	158
BamHI	GGATCC	1 cut(s)	260
BanI	GGYRCC	2 cut(s)	162, 449
BanII	GRGCYC	3 cut(s)	218, 593, 638
BarI	GAAGNNNNNNTAC	2 cut(s)	1839, 1871
BauI	CACGAG	1 cut(s)	2070
BbsI	GAAGAC	2 cut(s)	136, 1847
Bbv12I	GWGCWC	3 cut(s)	593, 638, 1869
BbvI	GCAGC	4 cut(s)	958, 1009, 1375, 1667
BccI	CCATC	5 cut(s)	664, 713, 1217, 1291, 1567
BceAI	ACGGC	1 cut(s)	400
BciT130I	CCWGG	1 cut(s)	272
BciVI	GTATCC	1 cut(s)	429
BclI	TGATCA	3 cut(s)	529, 1120, 1606
BcnI	CCSGG	7 cut(s)	139, 140, 208, 347, 501, 729, 1521
BcoDI	GTCTC	5 cut(s)	616, 1080, 1138, 1327, 1778
BfaI	CTAG	6 cut(s)	657, 1235, 1469, 1566, 2007, 2099
BfoI	RGCGCY	1 cut(s)	453
BfuAI	ACCTGC	2 cut(s)	1544, 1786
BfuI	GTATCC	1 cut(s)	429
BisI	GCNGC	9 cut(s)	232, 249, 403, 448, 972, 1023, 1389, 1392, 1656
BlsI	GCNGC	9 cut(s)	233, 250, 404, 449, 973, 1024, 1390, 1393, 1657
Bme1390I	CCNGG	8 cut(s)	139, 140, 208, 272, 347, 501, 729, 1521
Bme18I	GGWCC	4 cut(s)	98, 318, 740, 1711
BmeT110I	CYCGRG	3 cut(s)	138, 477, 1368
BmgT120I	GGNCC	4 cut(s)	98, 318, 740, 1711
BmiI	GGNNCC	7 cut(s)	100, 164, 178, 217, 262, 451, 1628
BmrFI	CCNGG	8 cut(s)	139, 140, 208, 272, 347, 501, 729, 1521
BmrI	ACTGGG	2 cut(s)	62, 310
BmsI	GCATC	1 cut(s)	1153
BmuI	ACTGGG	2 cut(s)	62, 310
BpiI	GAAGAC	2 cut(s)	136, 1847
BpmI	CTGGAG	1 cut(s)	1966
Bpu14I	TTCGAA	1 cut(s)	761
BpuMI	CCSGG	7 cut(s)	139, 140, 208, 347, 501, 729, 1521
BsaHI	GRCGYC	2 cut(s)	11, 450
BsaI	GGTCTC	2 cut(s)	1138, 1327
BsaJI	CCNNGG	8 cut(s)	138, 172, 331, 346, 665, 728, 951, 1239
BsaWI	WCCGGW	2 cut(s)	600, 1250
BsaXI	ACNNNNNCTCC	4 cut(s)	367, 397, 724, 754
Bsc4I	CCNNNNNNNGG	5 cut(s)	332, 352, 1316, 1375, 1430
Bse118I	RCCGGY	1 cut(s)	600
Bse1I	ACTGG	6 cut(s)	68, 305, 768, 1261, 1620, 1935
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	2 cut(s)	1869, 1923
BseBI	CCWGG	1 cut(s)	272
BseDI	CCNNGG	8 cut(s)	138, 172, 331, 346, 665, 728, 951, 1239
BseLI	CCNNNNNNNGG	5 cut(s)	332, 352, 1316, 1375, 1430
BseMI	GCAATG	2 cut(s)	1869, 1923
BseMII	CTCAG	2 cut(s)	172, 284
BseNI	ACTGG	6 cut(s)	68, 305, 768, 1261, 1620, 1935
BseRI	GAGGAG	6 cut(s)	35, 38, 105, 748, 807, 923
BseXI	GCAGC	4 cut(s)	958, 1009, 1375, 1667
BseYI	CCCAGC	1 cut(s)	180
BsgI	GTGCAG	2 cut(s)	1891, 2052
Bsh1236I	CGCG	4 cut(s)	229, 231, 333, 1323
Bsh1285I	CGRYCG	1 cut(s)	255
BshFI	GGCC	1 cut(s)	345
BshNI	GGYRCC	2 cut(s)	162, 449
BshTI	ACCGGT	1 cut(s)	600
BsiEI	CGRYCG	1 cut(s)	255
BsiHKAI	GWGCWC	3 cut(s)	593, 638, 1869
BsiHKCI	CYCGRG	3 cut(s)	138, 477, 1368
BsiSI	CCGG	9 cut(s)	139, 207, 346, 422, 500, 601, 728, 1251, 1520
BslFI	GGGAC	1 cut(s)	713
BslI	CCNNNNNNNGG	5 cut(s)	332, 352, 1316, 1375, 1430
BsmAI	GTCTC	5 cut(s)	616, 1080, 1138, 1327, 1778
BsmBI	CGTCTC	1 cut(s)	616
BsmFI	GGGAC	1 cut(s)	713
BsmI	GAATGC	2 cut(s)	769, 1271
BsnI	GGCC	1 cut(s)	345
Bso31I	GGTCTC	2 cut(s)	1138, 1327
BsoBI	CYCGRG	3 cut(s)	138, 477, 1368
Bsp119I	TTCGAA	1 cut(s)	761
Bsp1286I	GDGCHC	4 cut(s)	218, 593, 638, 1869
Bsp143I	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
BspANI	GGCC	1 cut(s)	345
BspCNI	CTCAG	2 cut(s)	171, 285
BspFNI	CGCG	4 cut(s)	229, 231, 333, 1323
BspHI	TCATGA	2 cut(s)	532, 1747
BspLI	GGNNCC	7 cut(s)	100, 164, 178, 217, 262, 451, 1628
BspMI	ACCTGC	2 cut(s)	1544, 1786
BspPI	GGATC	3 cut(s)	255, 268, 1874
BspT104I	TTCGAA	1 cut(s)	761
BspT107I	GGYRCC	2 cut(s)	162, 449
BspTNI	GGTCTC	2 cut(s)	1138, 1327
BsrBI	CCGCTC	2 cut(s)	113, 151
BsrDI	GCAATG	2 cut(s)	1869, 1923
BsrFI	RCCGGY	1 cut(s)	600
BsrI	ACTGG	6 cut(s)	68, 305, 768, 1261, 1620, 1935
BssAI	RCCGGY	1 cut(s)	600
BssECI	CCNNGG	8 cut(s)	138, 172, 331, 346, 665, 728, 951, 1239
BssMI	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
BssNI	GRCGYC	2 cut(s)	11, 450
BssSI	CACGAG	1 cut(s)	2070
BssT1I	CCWWGG	1 cut(s)	1239
Bst2BI	CACGAG	1 cut(s)	2070
Bst2UI	CCWGG	1 cut(s)	272
Bst4CI	ACNGT	8 cut(s)	365, 968, 1143, 1186, 1267, 1686, 1699, 1765
Bst6I	CTCTTC	3 cut(s)	1209, 1701, 1776
BstACI	GRCGYC	2 cut(s)	11, 450
BstAPI	GCANNNNNTGC	1 cut(s)	1559
BstBI	TTCGAA	1 cut(s)	761
BstC8I	GCNNGC	3 cut(s)	1377, 1484, 1488
BstDEI	CTNAG	3 cut(s)	158, 293, 786
BstDSI	CCRYGG	2 cut(s)	331, 665
BstFNI	CGCG	4 cut(s)	229, 231, 333, 1323
BstH2I	RGCGCY	1 cut(s)	453
BstHHI	GCGC	4 cut(s)	186, 231, 452, 1795
BstKTI	GATC	8 cut(s)	263, 532, 1123, 1327, 1537, 1609, 1747, 1882
BstMAI	GTCTC	5 cut(s)	616, 1080, 1138, 1327, 1778
BstMBI	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
BstMCI	CGRYCG	1 cut(s)	255
BstMWI	GCNNNNNNNGC	5 cut(s)	40, 183, 1385, 1397, 1559
BstNI	CCWGG	1 cut(s)	272
BstNSI	RCATGY	2 cut(s)	1486, 1793
BstSCI	CCNGG	8 cut(s)	137, 138, 206, 270, 345, 499, 727, 1519
BstUI	CGCG	4 cut(s)	229, 231, 333, 1323
BstV1I	GCAGC	4 cut(s)	958, 1009, 1375, 1667
BstV2I	GAAGAC	2 cut(s)	136, 1847
BstX2I	RGATCY	1 cut(s)	260
BstXI	CCANNNNNNTGG	1 cut(s)	271
BstYI	RGATCY	1 cut(s)	260
Bsu36I	CCTNAGG	1 cut(s)	158
BsuI	GTATCC	1 cut(s)	429
BsuRI	GGCC	1 cut(s)	345
BtgI	CCRYGG	2 cut(s)	331, 665
BtsI	GCAGTG	1 cut(s)	1783
BtsIMutI	CAGTG	4 cut(s)	973, 1130, 1139, 1783
BveI	ACCTGC	2 cut(s)	1544, 1786
Cac8I	GCNNGC	3 cut(s)	1377, 1484, 1488
CciI	TCATGA	2 cut(s)	532, 1747
CfoI	GCGC	4 cut(s)	186, 231, 452, 1795
Cfr10I	RCCGGY	1 cut(s)	600
Cfr13I	GGNCC	4 cut(s)	98, 318, 740, 1711
Cfr42I	CCGCGG	1 cut(s)	334
Cfr9I	CCCGGG	1 cut(s)	138
CseI	GACGC	1 cut(s)	1329
Csp6I	GTAC	6 cut(s)	125, 163, 994, 1190, 1349, 1455
CspAI	ACCGGT	1 cut(s)	600
CspCI	CAANNNNNGTGG	2 cut(s)	1128, 1163
CviAII	CATG	6 cut(s)	88, 533, 1180, 1483, 1748, 1790
CviQI	GTAC	6 cut(s)	125, 163, 994, 1190, 1349, 1455
DdeI	CTNAG	3 cut(s)	158, 293, 786
DinI	GGCGCC	1 cut(s)	451
DpnI	GATC	8 cut(s)	262, 531, 1122, 1326, 1536, 1608, 1746, 1881
DpnII	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
EaeI	YGGCCR	1 cut(s)	343
Eam1104I	CTCTTC	3 cut(s)	1209, 1701, 1776
EarI	CTCTTC	3 cut(s)	1209, 1701, 1776
EciI	GGCGGA	3 cut(s)	143, 302, 552
Ecl136II	GAGCTC	2 cut(s)	591, 636
Eco130I	CCWWGG	1 cut(s)	1239
Eco24I	GRGCYC	3 cut(s)	218, 593, 638
Eco31I	GGTCTC	2 cut(s)	1138, 1327
Eco47I	GGWCC	4 cut(s)	98, 318, 740, 1711
Eco53kI	GAGCTC	2 cut(s)	591, 636
Eco81I	CCTNAGG	1 cut(s)	158
Eco88I	CYCGRG	3 cut(s)	138, 477, 1368
EcoICRI	GAGCTC	2 cut(s)	591, 636
EcoRI	GAATTC	1 cut(s)	757
EcoRII	CCWGG	1 cut(s)	270
EcoT14I	CCWWGG	1 cut(s)	1239
EcoT38I	GRGCYC	3 cut(s)	218, 593, 638
EgeI	GGCGCC	1 cut(s)	451
EheI	GGCGCC	1 cut(s)	451
ErhI	CCWWGG	1 cut(s)	1239
Esp3I	CGTCTC	1 cut(s)	616
FaeI	CATG	6 cut(s)	91, 536, 1183, 1486, 1751, 1793
FaqI	GGGAC	1 cut(s)	713
FatI	CATG	6 cut(s)	87, 532, 1179, 1482, 1747, 1789
FauI	CCCGC	7 cut(s)	83, 136, 156, 206, 338, 1075, 1159
FbaI	TGATCA	3 cut(s)	529, 1120, 1606
FblI	GTMKAC	1 cut(s)	276
Fnu4HI	GCNGC	9 cut(s)	232, 249, 403, 448, 972, 1023, 1389, 1392, 1656
FriOI	GRGCYC	3 cut(s)	218, 593, 638
Fsp4HI	GCNGC	9 cut(s)	232, 249, 403, 448, 972, 1023, 1389, 1392, 1656
FspBI	CTAG	6 cut(s)	657, 1235, 1469, 1566, 2007, 2099
FspI	TGCGCA	1 cut(s)	1794
GlaI	GCGC	4 cut(s)	185, 230, 451, 1794
GluI	GCNGC	9 cut(s)	232, 249, 403, 448, 972, 1023, 1389, 1392, 1656
GsaI	CCCAGC	1 cut(s)	184
GsuI	CTGGAG	1 cut(s)	1966
HaeII	RGCGCY	1 cut(s)	453
HaeIII	GGCC	1 cut(s)	345
HapII	CCGG	9 cut(s)	139, 207, 346, 422, 500, 601, 728, 1251, 1520
HgaI	GACGC	1 cut(s)	1329
HhaI	GCGC	4 cut(s)	186, 231, 452, 1795
Hin1I	GRCGYC	2 cut(s)	11, 450
Hin1II	CATG	6 cut(s)	91, 536, 1183, 1486, 1751, 1793
Hin6I	GCGC	4 cut(s)	184, 229, 450, 1793
HinP1I	GCGC	4 cut(s)	184, 229, 450, 1793
HincII	GTYRAC	1 cut(s)	277
HindII	GTYRAC	1 cut(s)	277
HpaII	CCGG	9 cut(s)	139, 207, 346, 422, 500, 601, 728, 1251, 1520
HphI	GGTGA	6 cut(s)	476, 620, 925, 1063, 1299, 1961
Hpy166II	GTNNAC	3 cut(s)	24, 277, 509
Hpy8I	GTNNAC	3 cut(s)	24, 277, 509
Hpy99I	CGWCG	5 cut(s)	13, 16, 281, 284, 458
HpyCH4III	ACNGT	8 cut(s)	365, 968, 1143, 1186, 1267, 1686, 1699, 1765
HpyCH4IV	ACGT	2 cut(s)	11, 2091
HpyCH4V	TGCA	7 cut(s)	1025, 1271, 1486, 1562, 1908, 1916, 2069
HpyF10VI	GCNNNNNNNGC	5 cut(s)	40, 183, 1385, 1397, 1559
HpyF3I	CTNAG	3 cut(s)	158, 293, 786
HpySE526I	ACGT	2 cut(s)	11, 2091
Hsp92I	GRCGYC	2 cut(s)	11, 450
Hsp92II	CATG	6 cut(s)	91, 536, 1183, 1486, 1751, 1793
HspAI	GCGC	4 cut(s)	184, 229, 450, 1793
KasI	GGCGCC	1 cut(s)	449
KpnI	GGTACC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	529, 1120, 1606
KspI	CCGCGG	1 cut(s)	334
Kzo9I	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
LmnI	GCTCC	8 cut(s)	48, 118, 156, 182, 221, 289, 432, 1825
Lsp1109I	GCAGC	4 cut(s)	958, 1009, 1375, 1667
LweI	GCATC	1 cut(s)	1153
MaeI	CTAG	6 cut(s)	657, 1235, 1469, 1566, 2007, 2099
MaeII	ACGT	2 cut(s)	11, 2091
MaeIII	GTNAC	6 cut(s)	70, 296, 962, 1921, 2050, 2092
MalI	GATC	8 cut(s)	262, 531, 1122, 1326, 1536, 1608, 1746, 1881
MbiI	CCGCTC	2 cut(s)	113, 151
MboI	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
MboII	GAAGA	7 cut(s)	136, 728, 968, 1196, 1718, 1793, 1847
MfeI	CAATTG	1 cut(s)	1287
MflI	RGATCY	1 cut(s)	260
MhlI	GDGCHC	4 cut(s)	218, 593, 638, 1869
Mly113I	GGCGCC	1 cut(s)	450
MlyI	GAGTC	6 cut(s)	476, 620, 1063, 1082, 1088, 1118
MmeI	TCCRAC	1 cut(s)	353
MseI	TTAA	2 cut(s)	39, 1758
MslI	CAYNNNNRTG	3 cut(s)	29, 537, 1184
MspA1I	CMGCKG	4 cut(s)	333, 1022, 1082, 1658
MspI	CCGG	9 cut(s)	139, 207, 346, 422, 500, 601, 728, 1251, 1520
MspR9I	CCNGG	8 cut(s)	139, 140, 208, 272, 347, 501, 729, 1521
MunI	CAATTG	1 cut(s)	1287
Mva1269I	GAATGC	2 cut(s)	769, 1271
MvaI	CCWGG	1 cut(s)	272
MvnI	CGCG	4 cut(s)	229, 231, 333, 1323
MwoI	GCNNNNNNNGC	5 cut(s)	40, 183, 1385, 1397, 1559
NarI	GGCGCC	1 cut(s)	450
NciI	CCSGG	7 cut(s)	139, 140, 208, 347, 501, 729, 1521
NdeII	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
NlaIII	CATG	6 cut(s)	91, 536, 1183, 1486, 1751, 1793
NlaIV	GGNNCC	7 cut(s)	100, 164, 178, 217, 262, 451, 1628
NmeAIII	GCCGAG	2 cut(s)	153, 591
NmuCI	GTSAC	4 cut(s)	296, 962, 1921, 2050
NsbI	TGCGCA	1 cut(s)	1794
NspI	RCATGY	2 cut(s)	1486, 1793
NspV	TTCGAA	1 cut(s)	761
PaeI	GCATGC	1 cut(s)	1486
PagI	TCATGA	2 cut(s)	532, 1747
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	2 cut(s)	769, 1271
PfeI	GAWTC	6 cut(s)	356, 616, 809, 857, 1155, 1504
PflFI	GACNNNGTC	1 cut(s)	302
PfoI	TCCNGGA	1 cut(s)	499
PinAI	ACCGGT	1 cut(s)	600
PkrI	GCNGC	9 cut(s)	233, 250, 404, 449, 973, 1024, 1390, 1393, 1657
PleI	GAGTC	6 cut(s)	475, 619, 1062, 1082, 1088, 1117
PluTI	GGCGCC	1 cut(s)	453
PpsI	GAGTC	6 cut(s)	475, 619, 1062, 1082, 1088, 1117
Psp124BI	GAGCTC	2 cut(s)	593, 638
Psp6I	CCWGG	1 cut(s)	270
PspFI	CCCAGC	1 cut(s)	180
PspGI	CCWGG	1 cut(s)	270
PspN4I	GGNNCC	7 cut(s)	100, 164, 178, 217, 262, 451, 1628
PspPI	GGNCC	4 cut(s)	98, 318, 740, 1711
PsuI	RGATCY	1 cut(s)	260
PsyI	GACNNNGTC	1 cut(s)	302
PvuII	CAGCTG	2 cut(s)	1022, 1658
RsaI	GTAC	6 cut(s)	126, 164, 995, 1191, 1350, 1456
RsaNI	GTAC	6 cut(s)	125, 163, 994, 1190, 1349, 1455
RseI	CAYNNNNRTG	3 cut(s)	29, 537, 1184
SacI	GAGCTC	2 cut(s)	593, 638
SacII	CCGCGG	1 cut(s)	334
SalI	GTCGAC	1 cut(s)	275
SaqAI	TTAA	2 cut(s)	39, 1758
SatI	GCNGC	9 cut(s)	232, 249, 403, 448, 972, 1023, 1389, 1392, 1656
Sau3AI	GATC	8 cut(s)	260, 529, 1120, 1324, 1534, 1606, 1744, 1879
Sau96I	GGNCC	4 cut(s)	98, 318, 740, 1711
SchI	GAGTC	6 cut(s)	476, 620, 1063, 1082, 1088, 1118
ScrFI	CCNGG	8 cut(s)	139, 140, 208, 272, 347, 501, 729, 1521
SduI	GDGCHC	4 cut(s)	218, 593, 638, 1869
SfaNI	GCATC	1 cut(s)	1153
SfoI	GGCGCC	1 cut(s)	451
Sfr303I	CCGCGG	1 cut(s)	334
SfuI	TTCGAA	1 cut(s)	761
SgrBI	CCGCGG	1 cut(s)	334
SinI	GGWCC	4 cut(s)	98, 318, 740, 1711
SmaI	CCCGGG	1 cut(s)	140
SmiMI	CAYNNNNRTG	3 cut(s)	29, 537, 1184
SphI	GCATGC	1 cut(s)	1486
SspDI	GGCGCC	1 cut(s)	449
SspMI	CTAG	6 cut(s)	657, 1235, 1469, 1566, 2007, 2099
SstI	GAGCTC	2 cut(s)	593, 638
StyD4I	CCNGG	8 cut(s)	137, 138, 206, 270, 345, 499, 727, 1519
StyI	CCWWGG	1 cut(s)	1239
TaaI	ACNGT	8 cut(s)	365, 968, 1143, 1186, 1267, 1686, 1699, 1765
TaiI	ACGT	2 cut(s)	14, 2094
TaqI	TCGA	9 cut(s)	118, 276, 470, 587, 593, 614, 761, 773, 860
TaqII	GACCGA	3 cut(s)	335, 480, 1120
TatI	WGTACW	1 cut(s)	1348
TauI	GCSGC	5 cut(s)	234, 251, 405, 450, 1394
TfiI	GAWTC	6 cut(s)	356, 616, 809, 857, 1155, 1504
Tru1I	TTAA	2 cut(s)	39, 1758
Tru9I	TTAA	2 cut(s)	39, 1758
TscAI	CASTG	4 cut(s)	973, 1130, 1146, 1783
TseFI	GTSAC	4 cut(s)	296, 962, 1921, 2050
TseI	GCWGC	4 cut(s)	971, 1022, 1388, 1655
Tsp45I	GTSAC	4 cut(s)	296, 962, 1921, 2050
TspDTI	ATGAA	5 cut(s)	17, 104, 1614, 1764, 2034
TspGWI	ACGGA	4 cut(s)	526, 682, 1619, 1703
TspMI	CCCGGG	1 cut(s)	138
TspRI	CASTG	4 cut(s)	973, 1130, 1146, 1783
Tth111I	GACNNNGTC	1 cut(s)	302
VpaK11BI	GGWCC	4 cut(s)	98, 318, 740, 1711
XapI	RAATTY	6 cut(s)	92, 692, 745, 757, 1751, 2021
XbaI	TCTAGA	1 cut(s)	1468
XceI	RCATGY	2 cut(s)	1486, 1793
XmaI	CCCGGG	1 cut(s)	138
XmiI	GTMKAC	1 cut(s)	276
XspI	CTAG	6 cut(s)	657, 1235, 1469, 1566, 2007, 2099
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.