Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Forward (+)

16352081 .. 16357573

Gene Structure 5493 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027883

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGAAAACGACGTCGCTTTCGTCCACTATTATGCCCGTTAAGCTCCTCCTCAACAACCCTTGTCTCCCAGTCACCCGCTTCCTCTCCATGAAATTCGGACCCTCCATTTCCGCTCCTCGACCCGTACCCGCTTTCTCCCGGGTCTTCCCGCTCCGCCTCAGGTACCTCGCCCTCGGCTCCCAGCGCACATTCTCCACCCGCACCTTCCGGACCCCAAGAACCGGGTCGGGCTCCCAATTCTCGCGCGGCTACGAGCGAAAGGCGGCGGTCGGGGGATCCAAGAGCCTGGTCGACGACGAGGCGGAGCTGAGTGACTGGGTCAGCGAGTTGAGGACCGACCCGACCCGCGGTGGCGGTGGCCGGGGAGAGGAATCGGACGGTGGTGGTCGGAGAGGTAGTAGGGTTAGAGATAGGGGCGGCGATAGAGAGCCGTATCCGGCGAAGCGGAGCAGGAGAGATAGCGGCGCCGACGAGTTTGGTGAGTCGAGAACTCGGGGAGGGTTTCGGTCTTTTCCGGGAAGTTCACGGATGAGTAAAAGGGATGATCATGATGATAGAGAAGGTAGGTCTTTTCCGCCGAGAAGCAATCGTGGGAGTTCGAGCTCGAATGACCGGTTTGGTGAGTCGAATCGGAGACGATTTCCGAGCTCGAATCCGTCGGCATTTACTAGAAATCCACGGATGGATAGAAGGTTTGATAACAAATTTGGGGACAATGCTGTGAAGGAAGATGAGTTTTCGGGGAGGAGAGGTCCGAATTTCAGAGGGAATTCGAATGCGAGTTCGAGTTCTTTGAGTAAGAGAGGAGGGAGAGAGGTGGATTCGGGTTTCAGACGAGGCGGTGAGGGGTTGAGGAAGGGTGGGAGAGTTTCGAGGAAGGAAAAGGAAATGCTTGTGGTGGATGATAGTGATGAGGATGAGGAGGTGAATGAGCTTGGTGTTGGTATTGGGGATTTGCTTACCGAGGAAGATAGTGATAGTGCTGCTTCGGATGAGGATGATGAGTATGAAGGATTGAAAGATAAGAGTTCAGCTGCATTGTTTGGTTCGGATAAGGAAGTGGTGAGTCAAAAGGCTGTGCCGAAAAGTTCAGCTGGAGACTCTGACTCTTATTTGAGTGAGTCAAGGTTTGATCAGTGTTCGGTCTCGCCACTGTCCTTGAAGGGAATCAAGGATGCGGGATATGAGAACATGACTGTGGTACAAGAGGCTACTCTTCCCATTATACTCAAAGGCAAAGATGTTCTAGCCAAGGCTAAAACCGGAACTGGAAAAACTGTTGCATTCTTGCTTCCATCAATTGAAGTTGTTGTAAAATCACCTCCTATTGGACGCGATCATAAGAGACCCCCAATTTCTGTACTTGTAATATGCCCAACCCGAGAGCTGGCGAGTCAAGCTGCAGCAGAAGCCAATAAATTGTTGAAGTATCATCCTTCTATTGGTGTTCAAGTTGTGATAGGAGGTACAAGACTTGCTCTAGAACAGAAACGCATGCAAGCAAACCCTTGCCAGATTCTTGTCGCTACACCGGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTCGCAACTAGGCTGATGGGTGTCAAAGTCCTCATTCTTGATGAAGCTGATCATCTACTGGATATGGGGTTCCGTAAAGACATTGAAAGGATTATAGCAGCTGTTCCAAAACAGCGACAGACACTACTGTTCTCTGCCACCGTTCCAGAAGAGGTCCGTCAAATCTGTCATATCGCTTTGAAAAGAGATCATGAATTTATTAACACGGTTCAGGAAGGCAGTGAAGAGACACATGCGCAGGTCAGACAAACCCATTTGGTTGCTCCTTTGGACAAACACTTTTCCTTTGTATATAGTCTTCTTAAAGAGCACATTGCTGACGATCCTGACTATAAGGTTCTTGTATTCTGCACTACTGCAATGGTCACTCGACTGGTTGCTGACCTTCTTGGTGAGCTGAACTTGAATGTTAGAGAAATCCACTCCAGAAAGCCACAGAGTTATAGAACTAGGGTTTCCGATGAATTTCGGAAATCAAAGGGTCTTATCCTTGTGACTTCTGATGTATCTGCACGAGGGGTTGATTATCCAGACGTTACTCTAGTCATACAGGTGGGCATTCCAGCTGATAGACAACAGTATATTCACCGACTTGGTAGAACTGGCCGTAAAGGCAAAGAAGGGCAAGGAATATTGCTACTGGCTCCTTGGGAGGAATTTTTCTTATCCACTATTAAAGATTTGCCAATGACCAAGGCTCCTGTACCTTTAGTTGATCCAGATACGACCAAGAAGGTAGCACGAGCTCTATCCCAAGTGGACATGAAGAACAAAGAAGCAGCATACCAAGCGTGGCTGGGGTATTACAATTCAAATAAGAAAATAGGGCGTGATAAGCATAGGCTTGTGGAGCTTGCTAATGAGTTTAGCCGAAGCATGGGATTGGACAACCCTCCAGCCATTGCGAAGCTTGTTCTTGGTAAAATGGGTCTTAAGAATGTCCCTGGACTGCGTTCCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

844

Amino Acids

93.09

Weight (kDa)

9.53

Isoelectric Point (pI)

46.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	400 - 577	2.2e-45	DEAD/DEAH box helicase
ResIII	PF04851	411 - 572	5.9e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	623 - 728	3e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	1806
Acc36I	ACCTGC	2 cut(s)	1556, 1798
Acc65I	GGTACC	1 cut(s)	162
AccB1I	GGYRCC	2 cut(s)	162, 464
AccBSI	CCGCTC	2 cut(s)	113, 151
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	4 cut(s)	244, 246, 348, 1335
AccIII	TCCGGA	1 cut(s)	207
AclWI	GGATC	4 cut(s)	270, 283, 1886, 2279
AcoI	YGGCCR	2 cut(s)	358, 2173
AcsI	RAATTY	7 cut(s)	92, 704, 757, 769, 1763, 2033, 2225
AcyI	GRCGYC	2 cut(s)	11, 465
AfaI	GTAC	6 cut(s)	126, 164, 1203, 1362, 1468, 2274
AfiI	CCNNNNNNNGG	7 cut(s)	221, 347, 367, 1328, 1387, 1442, 2446
AflII	CTTAAG	1 cut(s)	2501
AgeI	ACCGGT	1 cut(s)	612
AgsI	TTSAA	9 cut(s)	1018, 1162, 1304, 1426, 1451, 1655, 1750, 1975, 2382
AjnI	CCWGG	2 cut(s)	285, 2512
AloI	GAACNNNNNNTCC	2 cut(s)	1622, 1654
Alw21I	GWGCWC	4 cut(s)	605, 650, 1881, 2317
Alw26I	GTCTC	6 cut(s)	68, 628, 1092, 1150, 1339, 1790
AlwI	GGATC	4 cut(s)	270, 283, 1886, 2279
Ama87I	CYCGRG	3 cut(s)	138, 492, 1380
Aor13HI	TCCGGA	1 cut(s)	207
AoxI	GGCC	2 cut(s)	358, 2173
ApeKI	GCWGC	6 cut(s)	983, 1034, 1400, 1403, 1667, 2348
ApoI	RAATTY	7 cut(s)	92, 704, 757, 769, 1763, 2033, 2225
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AsiGI	ACCGGT	1 cut(s)	612
Asp718I	GGTACC	1 cut(s)	162
AspLEI	GCGC	4 cut(s)	186, 246, 467, 1807
AspS9I	GGNCC	5 cut(s)	98, 210, 333, 752, 1723
AsuC2I	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1533
AsuHPI	GGTGA	9 cut(s)	64, 491, 632, 854, 937, 1075, 1311, 1973, 2147
AsuII	TTCGAA	1 cut(s)	773
AvaI	CYCGRG	3 cut(s)	138, 492, 1380
AvaII	GGWCC	5 cut(s)	98, 210, 333, 752, 1723
AxyI	CCTNAGG	1 cut(s)	158
BamHI	GGATCC	1 cut(s)	275
BanI	GGYRCC	2 cut(s)	162, 464
BanII	GRGCYC	4 cut(s)	233, 605, 650, 2317
BarI	GAAGNNNNNNTAC	2 cut(s)	1851, 1883
BauI	CACGAG	2 cut(s)	2082, 2310
BbsI	GAAGAC	2 cut(s)	136, 1859
Bbv12I	GWGCWC	4 cut(s)	605, 650, 1881, 2317
BbvI	GCAGC	6 cut(s)	970, 1021, 1387, 1415, 1679, 2360
BccI	CCATC	3 cut(s)	676, 1303, 1579
BceAI	ACGGC	2 cut(s)	415, 2160
BciT130I	CCWGG	2 cut(s)	287, 2514
BciVI	GTATCC	1 cut(s)	444
BclI	TGATCA	3 cut(s)	544, 1132, 1618
BcnI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1533
BcoDI	GTCTC	6 cut(s)	68, 628, 1092, 1150, 1339, 1790
BfaI	CTAG	6 cut(s)	669, 1247, 1481, 1578, 2019, 2111
BfmI	CTRYAG	1 cut(s)	1401
BfoI	RGCGCY	1 cut(s)	468
BfrI	CTTAAG	1 cut(s)	2501
BfuAI	ACCTGC	2 cut(s)	1556, 1798
BfuI	GTATCC	1 cut(s)	444
BglI	GCCNNNNNGGC	1 cut(s)	2181
Bme1390I	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1533, 2514
Bme18I	GGWCC	5 cut(s)	98, 210, 333, 752, 1723
BmeT110I	CYCGRG	3 cut(s)	138, 492, 1380
BmgT120I	GGNCC	5 cut(s)	98, 210, 333, 752, 1723
BmrFI	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1533, 2514
BmrI	ACTGGG	2 cut(s)	62, 325
BmsI	GCATC	1 cut(s)	1165
BmuI	ACTGGG	2 cut(s)	62, 325
BpiI	GAAGAC	2 cut(s)	136, 1859
BpmI	CTGGAG	3 cut(s)	1116, 1978, 2448
Bpu14I	TTCGAA	1 cut(s)	773
BpuMI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1533
BsaHI	GRCGYC	2 cut(s)	11, 465
BsaI	GGTCTC	2 cut(s)	1150, 1339
BsaWI	WCCGGW	3 cut(s)	207, 612, 1262
BsaXI	ACNNNNNCTCC	6 cut(s)	382, 412, 736, 766, 2251, 2281
Bsc4I	CCNNNNNNNGG	7 cut(s)	221, 347, 367, 1328, 1387, 1442, 2446
Bse118I	RCCGGY	1 cut(s)	612
Bse1I	ACTGG	7 cut(s)	68, 320, 1273, 1632, 1947, 2176, 2214
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	3 cut(s)	1881, 1935, 2469
BseAI	TCCGGA	1 cut(s)	207
BseBI	CCWGG	2 cut(s)	287, 2514
BseGI	GGATG	9 cut(s)	534, 547, 687, 907, 922, 997, 1003, 1180, 1432
BseLI	CCNNNNNNNGG	7 cut(s)	221, 347, 367, 1328, 1387, 1442, 2446
BseMI	GCAATG	3 cut(s)	1881, 1935, 2469
BseMII	CTCAG	2 cut(s)	172, 299
BseNI	ACTGG	7 cut(s)	68, 320, 1273, 1632, 1947, 2176, 2214
BseRI	GAGGAG	6 cut(s)	35, 38, 105, 760, 819, 935
BseXI	GCAGC	6 cut(s)	970, 1021, 1387, 1415, 1679, 2360
BseYI	CCCAGC	2 cut(s)	180, 2365
BsgI	GTGCAG	2 cut(s)	1903, 2064
Bsh1236I	CGCG	4 cut(s)	244, 246, 348, 1335
Bsh1285I	CGRYCG	1 cut(s)	270
BshFI	GGCC	2 cut(s)	360, 2175
BshNI	GGYRCC	2 cut(s)	162, 464
BshTI	ACCGGT	1 cut(s)	612
BsiEI	CGRYCG	1 cut(s)	270
BsiHKAI	GWGCWC	4 cut(s)	605, 650, 1881, 2317
BsiHKCI	CYCGRG	3 cut(s)	138, 492, 1380
BsiSI	CCGG	9 cut(s)	139, 208, 222, 361, 437, 515, 613, 1263, 1532
BslFI	GGGAC	2 cut(s)	725, 2495
BslI	CCNNNNNNNGG	7 cut(s)	221, 347, 367, 1328, 1387, 1442, 2446
BsmAI	GTCTC	6 cut(s)	68, 628, 1092, 1150, 1339, 1790
BsmBI	CGTCTC	1 cut(s)	628
BsmFI	GGGAC	2 cut(s)	725, 2495
BsmI	GAATGC	3 cut(s)	781, 1283, 2127
BsnI	GGCC	2 cut(s)	360, 2175
Bso31I	GGTCTC	2 cut(s)	1150, 1339
BsoBI	CYCGRG	3 cut(s)	138, 492, 1380
Bsp119I	TTCGAA	1 cut(s)	773
Bsp1286I	GDGCHC	5 cut(s)	233, 605, 650, 1881, 2317
Bsp13I	TCCGGA	1 cut(s)	207
Bsp143I	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
BspANI	GGCC	2 cut(s)	360, 2175
BspCNI	CTCAG	2 cut(s)	171, 300
BspEI	TCCGGA	1 cut(s)	207
BspFNI	CGCG	4 cut(s)	244, 246, 348, 1335
BspHI	TCATGA	2 cut(s)	547, 1759
BspMAI	CTGCAG	1 cut(s)	1405
BspMI	ACCTGC	2 cut(s)	1556, 1798
BspPI	GGATC	4 cut(s)	270, 283, 1886, 2279
BspT104I	TTCGAA	1 cut(s)	773
BspT107I	GGYRCC	2 cut(s)	162, 464
BspTI	CTTAAG	1 cut(s)	2501
BspTNI	GGTCTC	2 cut(s)	1150, 1339
BsrBI	CCGCTC	2 cut(s)	113, 151
BsrDI	GCAATG	3 cut(s)	1881, 1935, 2469
BsrFI	RCCGGY	1 cut(s)	612
BsrI	ACTGG	7 cut(s)	68, 320, 1273, 1632, 1947, 2176, 2214
BssAI	RCCGGY	1 cut(s)	612
BssMI	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
BssNI	GRCGYC	2 cut(s)	11, 465
BssSI	CACGAG	2 cut(s)	2082, 2310
BssT1I	CCWWGG	3 cut(s)	1251, 2216, 2262
Bst2BI	CACGAG	2 cut(s)	2082, 2310
Bst2UI	CCWGG	2 cut(s)	287, 2514
Bst4CI	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1698, 1711, 1777, 2148
Bst6I	CTCTTC	3 cut(s)	1221, 1713, 1788
BstACI	GRCGYC	2 cut(s)	11, 465
BstAFI	CTTAAG	1 cut(s)	2501
BstBI	TTCGAA	1 cut(s)	773
BstC8I	GCNNGC	4 cut(s)	1389, 1496, 1500, 2424
BstDEI	CTNAG	2 cut(s)	158, 308
BstDSI	CCRYGG	2 cut(s)	346, 677
BstF5I	GGATG	9 cut(s)	534, 547, 687, 907, 922, 997, 1003, 1180, 1432
BstFNI	CGCG	4 cut(s)	244, 246, 348, 1335
BstH2I	RGCGCY	1 cut(s)	468
BstHHI	GCGC	4 cut(s)	186, 246, 467, 1807
BstKTI	GATC	9 cut(s)	278, 547, 1135, 1339, 1549, 1621, 1759, 1894, 2287
BstMAI	GTCTC	6 cut(s)	68, 628, 1092, 1150, 1339, 1790
BstMBI	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
BstMCI	CGRYCG	1 cut(s)	270
BstMWI	GCNNNNNNNGC	9 cut(s)	40, 183, 1397, 1409, 1571, 2181, 2357, 2404, 2419
BstNI	CCWGG	2 cut(s)	287, 2514
BstNSI	RCATGY	2 cut(s)	1498, 1805
BstSCI	CCNGG	8 cut(s)	137, 138, 221, 285, 360, 514, 1531, 2512
BstSFI	CTRYAG	1 cut(s)	1401
BstUI	CGCG	4 cut(s)	244, 246, 348, 1335
BstV1I	GCAGC	6 cut(s)	970, 1021, 1387, 1415, 1679, 2360
BstV2I	GAAGAC	2 cut(s)	136, 1859
BstX2I	RGATCY	1 cut(s)	275
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	275
Bsu36I	CCTNAGG	1 cut(s)	158
BsuI	GTATCC	1 cut(s)	444
BsuRI	GGCC	2 cut(s)	360, 2175
BtgI	CCRYGG	2 cut(s)	346, 677
BtsCI	GGATG	9 cut(s)	534, 547, 687, 907, 922, 997, 1003, 1180, 1432
BtsI	GCAGTG	1 cut(s)	1795
BtsIMutI	CAGTG	3 cut(s)	1142, 1151, 1795
BveI	ACCTGC	2 cut(s)	1556, 1798
Cac8I	GCNNGC	4 cut(s)	1389, 1496, 1500, 2424
CciI	TCATGA	2 cut(s)	547, 1759
CfoI	GCGC	4 cut(s)	186, 246, 467, 1807
Cfr10I	RCCGGY	1 cut(s)	612
Cfr13I	GGNCC	5 cut(s)	98, 210, 333, 752, 1723
Cfr42I	CCGCGG	1 cut(s)	349
Cfr9I	CCCGGG	1 cut(s)	138
CseI	GACGC	1 cut(s)	1341
Csp6I	GTAC	6 cut(s)	125, 163, 1202, 1361, 1467, 2273
CspAI	ACCGGT	1 cut(s)	612
CspCI	CAANNNNNGTGG	2 cut(s)	1140, 1175
CviAII	CATG	8 cut(s)	88, 548, 1192, 1495, 1760, 1802, 2332, 2446
CviQI	GTAC	6 cut(s)	125, 163, 1202, 1361, 1467, 2273
DdeI	CTNAG	2 cut(s)	158, 308
DinI	GGCGCC	1 cut(s)	466
DpnI	GATC	9 cut(s)	277, 546, 1134, 1338, 1548, 1620, 1758, 1893, 2286
DpnII	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
EaeI	YGGCCR	2 cut(s)	358, 2173
Eam1104I	CTCTTC	3 cut(s)	1221, 1713, 1788
EarI	CTCTTC	3 cut(s)	1221, 1713, 1788
EciI	GGCGGA	3 cut(s)	143, 317, 564
Ecl136II	GAGCTC	3 cut(s)	603, 648, 2315
Eco130I	CCWWGG	3 cut(s)	1251, 2216, 2262
Eco24I	GRGCYC	4 cut(s)	233, 605, 650, 2317
Eco31I	GGTCTC	2 cut(s)	1150, 1339
Eco47I	GGWCC	5 cut(s)	98, 210, 333, 752, 1723
Eco53kI	GAGCTC	3 cut(s)	603, 648, 2315
Eco81I	CCTNAGG	1 cut(s)	158
Eco88I	CYCGRG	3 cut(s)	138, 492, 1380
EcoICRI	GAGCTC	3 cut(s)	603, 648, 2315
EcoRI	GAATTC	1 cut(s)	769
EcoRII	CCWGG	2 cut(s)	285, 2512
EcoT14I	CCWWGG	3 cut(s)	1251, 2216, 2262
EcoT38I	GRGCYC	4 cut(s)	233, 605, 650, 2317
EgeI	GGCGCC	1 cut(s)	466
EheI	GGCGCC	1 cut(s)	466
ErhI	CCWWGG	3 cut(s)	1251, 2216, 2262
Esp3I	CGTCTC	1 cut(s)	628
FaeI	CATG	8 cut(s)	91, 551, 1195, 1498, 1763, 1805, 2335, 2449
FaqI	GGGAC	2 cut(s)	725, 2495
FatI	CATG	8 cut(s)	87, 547, 1191, 1494, 1759, 1801, 2331, 2445
FauI	CCCGC	6 cut(s)	83, 136, 156, 206, 353, 1171
FbaI	TGATCA	3 cut(s)	544, 1132, 1618
FblI	GTMKAC	1 cut(s)	291
FokI	GGATG	9 cut(s)	541, 554, 694, 914, 929, 1004, 1010, 1187, 1419
FriOI	GRGCYC	4 cut(s)	233, 605, 650, 2317
FspBI	CTAG	6 cut(s)	669, 1247, 1481, 1578, 2019, 2111
FspI	TGCGCA	1 cut(s)	1806
GlaI	GCGC	4 cut(s)	185, 245, 466, 1806
GsaI	CCCAGC	2 cut(s)	184, 2369
GsuI	CTGGAG	3 cut(s)	1116, 1978, 2448
HaeII	RGCGCY	1 cut(s)	468
HaeIII	GGCC	2 cut(s)	360, 2175
HapII	CCGG	9 cut(s)	139, 208, 222, 361, 437, 515, 613, 1263, 1532
HgaI	GACGC	1 cut(s)	1341
HhaI	GCGC	4 cut(s)	186, 246, 467, 1807
Hin1I	GRCGYC	2 cut(s)	11, 465
Hin1II	CATG	8 cut(s)	91, 551, 1195, 1498, 1763, 1805, 2335, 2449
Hin6I	GCGC	4 cut(s)	184, 244, 465, 1805
HinP1I	GCGC	4 cut(s)	184, 244, 465, 1805
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	2477
HpaII	CCGG	9 cut(s)	139, 208, 222, 361, 437, 515, 613, 1263, 1532
HphI	GGTGA	9 cut(s)	64, 491, 632, 854, 937, 1075, 1311, 1973, 2147
Hpy166II	GTNNAC	4 cut(s)	24, 292, 524, 2329
Hpy8I	GTNNAC	4 cut(s)	24, 292, 524, 2329
Hpy99I	CGWCG	6 cut(s)	13, 16, 296, 299, 473, 661
HpyCH4III	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1698, 1711, 1777, 2148
HpyCH4IV	ACGT	2 cut(s)	11, 2103
HpyCH4V	TGCA	7 cut(s)	1037, 1283, 1403, 1498, 1920, 1928, 2081
HpyF10VI	GCNNNNNNNGC	9 cut(s)	40, 183, 1397, 1409, 1571, 2181, 2357, 2404, 2419
HpyF3I	CTNAG	2 cut(s)	158, 308
HpySE526I	ACGT	2 cut(s)	11, 2103
Hsp92I	GRCGYC	2 cut(s)	11, 465
Hsp92II	CATG	8 cut(s)	91, 551, 1195, 1498, 1763, 1805, 2335, 2449
HspAI	GCGC	4 cut(s)	184, 244, 465, 1805
KasI	GGCGCC	1 cut(s)	464
Kpn2I	TCCGGA	1 cut(s)	207
KpnI	GGTACC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	544, 1132, 1618
KspI	CCGCGG	1 cut(s)	349
Kzo9I	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
Lsp1109I	GCAGC	6 cut(s)	970, 1021, 1387, 1415, 1679, 2360
LweI	GCATC	1 cut(s)	1165
MaeI	CTAG	6 cut(s)	669, 1247, 1481, 1578, 2019, 2111
MaeII	ACGT	2 cut(s)	11, 2103
MaeIII	GTNAC	5 cut(s)	70, 311, 1933, 2062, 2104
MalI	GATC	9 cut(s)	277, 546, 1134, 1338, 1548, 1620, 1758, 1893, 2286
MbiI	CCGCTC	2 cut(s)	113, 151
MboI	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
MboII	GAAGA	8 cut(s)	136, 740, 980, 1208, 1730, 1805, 1859, 2347
MfeI	CAATTG	1 cut(s)	1299
MflI	RGATCY	1 cut(s)	275
MhlI	GDGCHC	5 cut(s)	233, 605, 650, 1881, 2317
Mly113I	GGCGCC	1 cut(s)	465
MlyI	GAGTC	7 cut(s)	491, 632, 1075, 1094, 1100, 1130, 1402
MmeI	TCCRAC	1 cut(s)	368
MroI	TCCGGA	1 cut(s)	207
MseI	TTAA	5 cut(s)	39, 1770, 1872, 2244, 2502
MslI	CAYNNNNRTG	3 cut(s)	29, 1196, 2120
MspA1I	CMGCKG	5 cut(s)	348, 1034, 1094, 1670, 2135
MspCI	CTTAAG	1 cut(s)	2501
MspI	CCGG	9 cut(s)	139, 208, 222, 361, 437, 515, 613, 1263, 1532
MspR9I	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1533, 2514
MunI	CAATTG	1 cut(s)	1299
Mva1269I	GAATGC	3 cut(s)	781, 1283, 2127
MvaI	CCWGG	2 cut(s)	287, 2514
MvnI	CGCG	4 cut(s)	244, 246, 348, 1335
MwoI	GCNNNNNNNGC	9 cut(s)	40, 183, 1397, 1409, 1571, 2181, 2357, 2404, 2419
NarI	GGCGCC	1 cut(s)	465
NciI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1533
NdeII	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
NlaIII	CATG	8 cut(s)	91, 551, 1195, 1498, 1763, 1805, 2335, 2449
NmeAIII	GCCGAG	2 cut(s)	153, 603
NmuCI	GTSAC	4 cut(s)	70, 311, 1933, 2062
NsbI	TGCGCA	1 cut(s)	1806
NspI	RCATGY	2 cut(s)	1498, 1805
NspV	TTCGAA	1 cut(s)	773
PaeI	GCATGC	1 cut(s)	1498
PagI	TCATGA	2 cut(s)	547, 1759
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	3 cut(s)	781, 1283, 2127
PfeI	GAWTC	6 cut(s)	371, 628, 652, 821, 1167, 1516
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	1 cut(s)	514
PinAI	ACCGGT	1 cut(s)	612
PleI	GAGTC	7 cut(s)	490, 631, 1074, 1094, 1100, 1129, 1401
PluTI	GGCGCC	1 cut(s)	468
PpsI	GAGTC	7 cut(s)	490, 631, 1074, 1094, 1100, 1129, 1401
Psp124BI	GAGCTC	3 cut(s)	605, 650, 2317
Psp6I	CCWGG	2 cut(s)	285, 2512
PspFI	CCCAGC	2 cut(s)	180, 2365
PspGI	CCWGG	2 cut(s)	285, 2512
PspPI	GGNCC	5 cut(s)	98, 210, 333, 752, 1723
PstI	CTGCAG	1 cut(s)	1405
PsuI	RGATCY	1 cut(s)	275
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	4 cut(s)	1034, 1094, 1670, 2135
RsaI	GTAC	6 cut(s)	126, 164, 1203, 1362, 1468, 2274
RsaNI	GTAC	6 cut(s)	125, 163, 1202, 1361, 1467, 2273
RseI	CAYNNNNRTG	3 cut(s)	29, 1196, 2120
SacI	GAGCTC	3 cut(s)	605, 650, 2317
SacII	CCGCGG	1 cut(s)	349
SalI	GTCGAC	1 cut(s)	290
SaqAI	TTAA	5 cut(s)	39, 1770, 1872, 2244, 2502
Sau3AI	GATC	9 cut(s)	275, 544, 1132, 1336, 1546, 1618, 1756, 1891, 2284
Sau96I	GGNCC	5 cut(s)	98, 210, 333, 752, 1723
SchI	GAGTC	7 cut(s)	491, 632, 1075, 1094, 1100, 1130, 1402
ScrFI	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1533, 2514
SduI	GDGCHC	5 cut(s)	233, 605, 650, 1881, 2317
SfaNI	GCATC	1 cut(s)	1165
SfcI	CTRYAG	1 cut(s)	1401
SfoI	GGCGCC	1 cut(s)	466
Sfr303I	CCGCGG	1 cut(s)	349
SfuI	TTCGAA	1 cut(s)	773
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	5 cut(s)	98, 210, 333, 752, 1723
SmaI	CCCGGG	1 cut(s)	140
SmiMI	CAYNNNNRTG	3 cut(s)	29, 1196, 2120
SmlI	CTYRAG	1 cut(s)	2501
SmoI	CTYRAG	1 cut(s)	2501
SphI	GCATGC	1 cut(s)	1498
SspDI	GGCGCC	1 cut(s)	464
SspI	AATATT	1 cut(s)	2202
SspMI	CTAG	6 cut(s)	669, 1247, 1481, 1578, 2019, 2111
SstI	GAGCTC	3 cut(s)	605, 650, 2317
StyD4I	CCNGG	8 cut(s)	137, 138, 221, 285, 360, 514, 1531, 2512
StyI	CCWWGG	3 cut(s)	1251, 2216, 2262
TaaI	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1698, 1711, 1777, 2148
TaiI	ACGT	2 cut(s)	14, 2106
TaqII	GACCGA	3 cut(s)	350, 495, 1132
TatI	WGTACW	1 cut(s)	1360
TauI	GCSGC	4 cut(s)	249, 266, 420, 465
TfiI	GAWTC	6 cut(s)	371, 628, 652, 821, 1167, 1516
Tru1I	TTAA	5 cut(s)	39, 1770, 1872, 2244, 2502
Tru9I	TTAA	5 cut(s)	39, 1770, 1872, 2244, 2502
TscAI	CASTG	3 cut(s)	1142, 1158, 1795
TseFI	GTSAC	4 cut(s)	70, 311, 1933, 2062
TseI	GCWGC	6 cut(s)	983, 1034, 1400, 1403, 1667, 2348
Tsp45I	GTSAC	4 cut(s)	70, 311, 1933, 2062
TspDTI	ATGAA	7 cut(s)	17, 104, 1023, 1626, 1776, 2046, 2348
TspGWI	ACGGA	5 cut(s)	541, 645, 694, 1631, 1715
TspMI	CCCGGG	1 cut(s)	138
TspRI	CASTG	3 cut(s)	1142, 1158, 1795
Tth111I	GACNNNGTC	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	2501
VpaK11BI	GGWCC	5 cut(s)	98, 210, 333, 752, 1723
XapI	RAATTY	7 cut(s)	92, 704, 757, 769, 1763, 2033, 2225
XbaI	TCTAGA	1 cut(s)	1480
XceI	RCATGY	2 cut(s)	1498, 1805
XmaI	CCCGGG	1 cut(s)	138
XmiI	GTMKAC	1 cut(s)	291
XspI	CTAG	6 cut(s)	669, 1247, 1481, 1578, 2019, 2111
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.