Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Reverse (-)

37853615 .. 37857946

Gene Structure 4332 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG249400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

ATGAAAACGACGTCGCTTTCGTCCACTATTATGCCCGTTAAGCTCCTCCTCAACAACCCTTCTCTCCCAGTAACCCGCTTCCTCTCCATGAAATTCGGACCCTCCATTTCCGCTCCTCGACCCGTACCCGCTTTCTCCCGGGTCTTCCCGCTCCGCCTCAGGTACCTCGCCCTCGGCTCCCAGCGCACATTCTCCACCCGCGCCTTCCGGACCCCAAGAACCGGGTCGGGCTCCCAATTCTCGGGCGGCTACGAGCGGAAGGCGGCGGTCGGGGGATCCAAGAGCCTGGTCGACGACGAGGCGGAGCTGAGTGACTGGGTCAGCGAGTTGAGGACCGACCCGACCCGCGGTGGCGGTGGCCGGGGAGAGGAATCGGACGGTGGTGGTCGGAGAGGTAGTAGGGTTAGAGATAGGGGCGGCGGCGATAGAGAGCCGTATCCGGCGAAGCGGAGCAGGAGAGATAGCGGCGCCGACGAGTTTGGTGAGTCGAGAACTCGGGGAGGGTTTCGGTCTTTTCCGGGAAGTTCACGGATGAGTAAAAGGGATGATCATGATGATAGAGAAGGTAGGTCATTTCCGCCGAGAAGCAATCGTGGGAGTTCGAGCTCGAATGACCGGTTTGGTGAGTCGAATCGGAGACGATTTCCGAGCTCGAATCCGTCGGCATTTACTAGAAATCCACGGATGGATAGAAGGTTTGATAACAAATTTGGGGACAATGCTGTGAAGGAAGATGAGTTTCCGGGGAGGAGAGGTCCGAATTTCAGAGGGAATTCGAATGCGAGTTCGAGTTCTTTGAGTAAGAGAGGAGGGAGAGAGGTGGATTCGGGTTTCAGACGAGGCGGTGAGGGGTTGAGGAAGGAAAAGGAAATGCTTGTGGTGGATGATAGTGATGAGGATGAGGAGGTGAATGAGCTTGGTGTTGGTATTGGGGATTTGCTTACCGAGGAAGATAGTGATGCTGCTGCTTCGGATGAGGATGATGAGTACGAAGGATTGAAAGATAAGAGTTCAGCTGCATTGTTTGGTTCGGATAAGGAAGTGGTGAGTCAGAAGGCTGTGCCGAAAAGTTCAGCTGGAGACTCTGACTCTTATTTGAGCGAGACAAGGTTTGATCAGTGCTCGGTCTCGCCACTGTCCTTGAAGGGAATCAAGGATGCGGGATATGAGAACATGACTGTGGTACAAGAGGCTACTCTTCCCATCATACTCAAAGGCAAGGATGTTCTAGCCAAGGCTAAAACCGGAACTGGAAAAACTGTTGCATTCTTGCTTCCATCAATTGAAGTTGTTGTAAAATCACCTCCTATTGGACGCGATCATAAGAGACCCCCAATGTCTGTACTTGTAATATGCCCAACCCGAGAGCTGGCGAGTCAAGCTGCAGCAGAAGCCAATAAATTGTTGAAGTATCATCCTTCTATTGGTGTTCAAGTTGTGATAGGAGGTACAAGACTTGCTCTAGAACAGAAACGCATGCAAGCAAACCCTTGCCAGATTCTTGTCGCTACACCGGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTCGCAACTAGGCTGATGGGTGTCAAAGTCCTCATCCTTGATGAAGCTGATCATCTACTGGATATGGGGTTCCGTAAAGACATTGAAAGGATTATAGCAGCTGTTCCAAAACAGCGACAGACACTACTGTTCTCTGCCACAGTTCCAGAAGAGGTCCGTCAAATCTGTCATATCGCTTTGAAAAGAGATCATGAATTTATTAACACGGTTCAGGAAGGCAGTGAAGAGACACATGCGCAGGTCAGACAAACCCATTTGGTTGCTCCTTTGGACAAACACTTTTCCCTTGTATATAGTCTTCTTAAAGAGCACATTGCTGACGATCCTGACTATAAGGTTCTCGTATTCTGCACTACTGCAATGGTCACACGACTGGTTGCTGACCTTCTTGGTGAGCTGAACTTGAATGTTAGAGAAATCCACTCCAGAAAGCCACAGAGTTATAGAACTAGGGTTTCCGATGAATTTCGGAAATCAAAGGGTCTTATCCTTGTGACTTCTGATGTATCTGCACGAGGGGTTGATTATCCAGACGTTACTCTAGTCATACAGGTGGGCATTCCAGCTGATAGACAACAGTATATTCACCGACTTGGTAGAACTGGCCGTAAAGGCAAAGAAGGGCAAGGAATATTGCTACTGGCTCCTTGGGAGGAATTTTTCTTATCCACTATTAAAGATTTACCAATGACCAAGGCTCCTGTACCTTTAGTTGATCCAGATACGACCAAGAAGGTAGCACGAGCTCTATCCCAAGTGGACATGAAGAACAAAGAAGCAGCATACCAAGCGTGGCTGGGGTATTACAATTCAAATAAGAAAATAGGGCGTGATAAGCATAGGCTTGTGGAGCTTGCTAATGAGTTTAGCCGAAGCATGGGATTGGACAACCCTCCAGCCATTGCCAAGCTTGTTCTTGGTAAAATGGGTCTTAAGAATGTCCCTGGACTGCGTTCCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

837

Amino Acids

92.25

Weight (kDa)

9.44

Isoelectric Point (pI)

46.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	394 - 571	2.6e-45	DEAD/DEAH box helicase
ResIII	PF04851	405 - 566	5.8e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	616 - 722	2.9e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	1788
Acc36I	ACCTGC	2 cut(s)	1538, 1780
Acc65I	GGTACC	1 cut(s)	162
AccB1I	GGYRCC	2 cut(s)	162, 467
AccBSI	CCGCTC	3 cut(s)	113, 151, 256
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	3 cut(s)	201, 348, 1317
AccIII	TCCGGA	1 cut(s)	207
AclWI	GGATC	4 cut(s)	270, 283, 1868, 2261
AcoI	YGGCCR	2 cut(s)	358, 2155
AcsI	RAATTY	7 cut(s)	92, 707, 760, 772, 1745, 2015, 2207
AcyI	GRCGYC	2 cut(s)	11, 468
AfaI	GTAC	7 cut(s)	126, 164, 989, 1185, 1344, 1450, 2256
AfiI	CCNNNNNNNGG	8 cut(s)	221, 241, 347, 367, 1310, 1369, 1424, 2428
AflII	CTTAAG	1 cut(s)	2483
AgeI	ACCGGT	1 cut(s)	615
AgsI	TTSAA	9 cut(s)	1000, 1144, 1286, 1408, 1433, 1637, 1732, 1957, 2364
AjnI	CCWGG	2 cut(s)	285, 2494
AloI	GAACNNNNNNTCC	2 cut(s)	1604, 1636
Alw21I	GWGCWC	5 cut(s)	608, 653, 1124, 1863, 2299
Alw26I	GTCTC	6 cut(s)	631, 1074, 1097, 1132, 1321, 1772
AlwI	GGATC	4 cut(s)	270, 283, 1868, 2261
Ama87I	CYCGRG	4 cut(s)	138, 241, 495, 1362
Aor13HI	TCCGGA	1 cut(s)	207
AoxI	GGCC	2 cut(s)	358, 2155
ApeKI	GCWGC	7 cut(s)	962, 965, 1016, 1382, 1385, 1649, 2330
ApoI	RAATTY	7 cut(s)	92, 707, 760, 772, 1745, 2015, 2207
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AsiGI	ACCGGT	1 cut(s)	615
Asp718I	GGTACC	1 cut(s)	162
AspLEI	GCGC	4 cut(s)	186, 203, 470, 1789
AspS9I	GGNCC	5 cut(s)	98, 210, 333, 755, 1705
AsuC2I	CCSGG	7 cut(s)	139, 140, 223, 362, 519, 744, 1515
AsuHPI	GGTGA	8 cut(s)	494, 635, 857, 919, 1057, 1293, 1955, 2129
AsuII	TTCGAA	1 cut(s)	776
AvaI	CYCGRG	4 cut(s)	138, 241, 495, 1362
AvaII	GGWCC	5 cut(s)	98, 210, 333, 755, 1705
AxyI	CCTNAGG	1 cut(s)	158
BamHI	GGATCC	1 cut(s)	275
BanI	GGYRCC	2 cut(s)	162, 467
BanII	GRGCYC	4 cut(s)	233, 608, 653, 2299
BarI	GAAGNNNNNNTAC	2 cut(s)	1833, 1865
BauI	CACGAG	2 cut(s)	2064, 2292
BbsI	GAAGAC	2 cut(s)	136, 1841
Bbv12I	GWGCWC	5 cut(s)	608, 653, 1124, 1863, 2299
BbvI	GCAGC	7 cut(s)	949, 952, 1003, 1369, 1397, 1661, 2342
BccI	CCATC	4 cut(s)	679, 1211, 1285, 1561
BceAI	ACGGC	2 cut(s)	418, 2142
BciT130I	CCWGG	2 cut(s)	287, 2496
BciVI	GTATCC	1 cut(s)	447
BclI	TGATCA	3 cut(s)	547, 1114, 1600
BcnI	CCSGG	7 cut(s)	139, 140, 223, 362, 519, 744, 1515
BcoDI	GTCTC	6 cut(s)	631, 1074, 1097, 1132, 1321, 1772
BfaI	CTAG	6 cut(s)	672, 1229, 1463, 1560, 2001, 2093
BfmI	CTRYAG	1 cut(s)	1383
BfoI	RGCGCY	1 cut(s)	471
BfrI	CTTAAG	1 cut(s)	2483
BfuAI	ACCTGC	2 cut(s)	1538, 1780
BfuI	GTATCC	1 cut(s)	447
BglI	GCCNNNNNGGC	1 cut(s)	2163
Bme1390I	CCNGG	9 cut(s)	139, 140, 223, 287, 362, 519, 744, 1515, 2496
Bme18I	GGWCC	5 cut(s)	98, 210, 333, 755, 1705
BmeT110I	CYCGRG	4 cut(s)	138, 241, 495, 1362
BmgT120I	GGNCC	5 cut(s)	98, 210, 333, 755, 1705
BmrFI	CCNGG	9 cut(s)	139, 140, 223, 287, 362, 519, 744, 1515, 2496
BmrI	ACTGGG	2 cut(s)	62, 325
BmsI	GCATC	2 cut(s)	949, 1147
BmuI	ACTGGG	2 cut(s)	62, 325
BpiI	GAAGAC	2 cut(s)	136, 1841
BpmI	CTGGAG	3 cut(s)	1098, 1960, 2430
Bpu14I	TTCGAA	1 cut(s)	776
BpuMI	CCSGG	7 cut(s)	139, 140, 223, 362, 519, 744, 1515
BsaHI	GRCGYC	2 cut(s)	11, 468
BsaI	GGTCTC	2 cut(s)	1132, 1321
BsaWI	WCCGGW	3 cut(s)	207, 615, 1244
BsaXI	ACNNNNNCTCC	6 cut(s)	382, 412, 739, 769, 2233, 2263
Bsc4I	CCNNNNNNNGG	8 cut(s)	221, 241, 347, 367, 1310, 1369, 1424, 2428
Bse118I	RCCGGY	1 cut(s)	615
Bse1I	ACTGG	7 cut(s)	68, 320, 1255, 1614, 1929, 2158, 2196
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	3 cut(s)	1863, 1917, 2451
BseAI	TCCGGA	1 cut(s)	207
BseBI	CCWGG	2 cut(s)	287, 2496
BseLI	CCNNNNNNNGG	8 cut(s)	221, 241, 347, 367, 1310, 1369, 1424, 2428
BseMI	GCAATG	3 cut(s)	1863, 1917, 2451
BseMII	CTCAG	2 cut(s)	172, 299
BseNI	ACTGG	7 cut(s)	68, 320, 1255, 1614, 1929, 2158, 2196
BseRI	GAGGAG	6 cut(s)	35, 38, 105, 763, 822, 917
BseXI	GCAGC	7 cut(s)	949, 952, 1003, 1369, 1397, 1661, 2342
BseYI	CCCAGC	2 cut(s)	180, 2347
BsgI	GTGCAG	2 cut(s)	1885, 2046
Bsh1236I	CGCG	3 cut(s)	201, 348, 1317
Bsh1285I	CGRYCG	1 cut(s)	270
BshFI	GGCC	2 cut(s)	360, 2157
BshNI	GGYRCC	2 cut(s)	162, 467
BshTI	ACCGGT	1 cut(s)	615
BsiEI	CGRYCG	1 cut(s)	270
BsiHKAI	GWGCWC	5 cut(s)	608, 653, 1124, 1863, 2299
BsiHKCI	CYCGRG	4 cut(s)	138, 241, 495, 1362
BslFI	GGGAC	2 cut(s)	728, 2477
BslI	CCNNNNNNNGG	8 cut(s)	221, 241, 347, 367, 1310, 1369, 1424, 2428
BsmAI	GTCTC	6 cut(s)	631, 1074, 1097, 1132, 1321, 1772
BsmBI	CGTCTC	1 cut(s)	631
BsmFI	GGGAC	2 cut(s)	728, 2477
BsmI	GAATGC	3 cut(s)	784, 1265, 2109
BsnI	GGCC	2 cut(s)	360, 2157
Bso31I	GGTCTC	2 cut(s)	1132, 1321
BsoBI	CYCGRG	4 cut(s)	138, 241, 495, 1362
Bsp119I	TTCGAA	1 cut(s)	776
Bsp1286I	GDGCHC	6 cut(s)	233, 608, 653, 1124, 1863, 2299
Bsp13I	TCCGGA	1 cut(s)	207
Bsp143I	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
BspANI	GGCC	2 cut(s)	360, 2157
BspCNI	CTCAG	2 cut(s)	171, 300
BspEI	TCCGGA	1 cut(s)	207
BspFNI	CGCG	3 cut(s)	201, 348, 1317
BspHI	TCATGA	2 cut(s)	550, 1741
BspMAI	CTGCAG	1 cut(s)	1387
BspMI	ACCTGC	2 cut(s)	1538, 1780
BspPI	GGATC	4 cut(s)	270, 283, 1868, 2261
BspT104I	TTCGAA	1 cut(s)	776
BspT107I	GGYRCC	2 cut(s)	162, 467
BspTI	CTTAAG	1 cut(s)	2483
BspTNI	GGTCTC	2 cut(s)	1132, 1321
BsrBI	CCGCTC	3 cut(s)	113, 151, 256
BsrDI	GCAATG	3 cut(s)	1863, 1917, 2451
BsrFI	RCCGGY	1 cut(s)	615
BsrI	ACTGG	7 cut(s)	68, 320, 1255, 1614, 1929, 2158, 2196
BssAI	RCCGGY	1 cut(s)	615
BssMI	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
BssNI	GRCGYC	2 cut(s)	11, 468
BssSI	CACGAG	2 cut(s)	2064, 2292
BssT1I	CCWWGG	3 cut(s)	1233, 2198, 2244
Bst2BI	CACGAG	2 cut(s)	2064, 2292
Bst2UI	CCWGG	2 cut(s)	287, 2496
Bst4CI	ACNGT	8 cut(s)	380, 1137, 1180, 1261, 1680, 1693, 1759, 2130
Bst6I	CTCTTC	3 cut(s)	1203, 1695, 1770
BstACI	GRCGYC	2 cut(s)	11, 468
BstAFI	CTTAAG	1 cut(s)	2483
BstBI	TTCGAA	1 cut(s)	776
BstC8I	GCNNGC	4 cut(s)	1371, 1478, 1482, 2406
BstDEI	CTNAG	2 cut(s)	158, 308
BstDSI	CCRYGG	2 cut(s)	346, 680
BstFNI	CGCG	3 cut(s)	201, 348, 1317
BstH2I	RGCGCY	1 cut(s)	471
BstHHI	GCGC	4 cut(s)	186, 203, 470, 1789
BstKTI	GATC	9 cut(s)	278, 550, 1117, 1321, 1531, 1603, 1741, 1876, 2269
BstMAI	GTCTC	6 cut(s)	631, 1074, 1097, 1132, 1321, 1772
BstMBI	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
BstMCI	CGRYCG	1 cut(s)	270
BstMWI	GCNNNNNNNGC	9 cut(s)	40, 183, 1379, 1391, 1553, 2163, 2339, 2386, 2401
BstNI	CCWGG	2 cut(s)	287, 2496
BstNSI	RCATGY	2 cut(s)	1480, 1787
BstSCI	CCNGG	9 cut(s)	137, 138, 221, 285, 360, 517, 742, 1513, 2494
BstSFI	CTRYAG	1 cut(s)	1383
BstUI	CGCG	3 cut(s)	201, 348, 1317
BstV1I	GCAGC	7 cut(s)	949, 952, 1003, 1369, 1397, 1661, 2342
BstV2I	GAAGAC	2 cut(s)	136, 1841
BstX2I	RGATCY	1 cut(s)	275
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	275
Bsu36I	CCTNAGG	1 cut(s)	158
BsuI	GTATCC	1 cut(s)	447
BsuRI	GGCC	2 cut(s)	360, 2157
BtgI	CCRYGG	2 cut(s)	346, 680
BtsI	GCAGTG	1 cut(s)	1777
BtsIMutI	CAGTG	3 cut(s)	1124, 1133, 1777
BveI	ACCTGC	2 cut(s)	1538, 1780
Cac8I	GCNNGC	4 cut(s)	1371, 1478, 1482, 2406
CciI	TCATGA	2 cut(s)	550, 1741
CfoI	GCGC	4 cut(s)	186, 203, 470, 1789
Cfr10I	RCCGGY	1 cut(s)	615
Cfr13I	GGNCC	5 cut(s)	98, 210, 333, 755, 1705
Cfr42I	CCGCGG	1 cut(s)	349
Cfr9I	CCCGGG	1 cut(s)	138
CseI	GACGC	1 cut(s)	1323
Csp6I	GTAC	7 cut(s)	125, 163, 988, 1184, 1343, 1449, 2255
CspAI	ACCGGT	1 cut(s)	615
CspCI	CAANNNNNGTGG	2 cut(s)	1122, 1157
CviAII	CATG	8 cut(s)	88, 551, 1174, 1477, 1742, 1784, 2314, 2428
CviQI	GTAC	7 cut(s)	125, 163, 988, 1184, 1343, 1449, 2255
DdeI	CTNAG	2 cut(s)	158, 308
DinI	GGCGCC	1 cut(s)	469
DpnI	GATC	9 cut(s)	277, 549, 1116, 1320, 1530, 1602, 1740, 1875, 2268
DpnII	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
EaeI	YGGCCR	2 cut(s)	358, 2155
Eam1104I	CTCTTC	3 cut(s)	1203, 1695, 1770
EarI	CTCTTC	3 cut(s)	1203, 1695, 1770
EciI	GGCGGA	3 cut(s)	143, 317, 567
Ecl136II	GAGCTC	3 cut(s)	606, 651, 2297
Eco130I	CCWWGG	3 cut(s)	1233, 2198, 2244
Eco24I	GRGCYC	4 cut(s)	233, 608, 653, 2299
Eco31I	GGTCTC	2 cut(s)	1132, 1321
Eco47I	GGWCC	5 cut(s)	98, 210, 333, 755, 1705
Eco53kI	GAGCTC	3 cut(s)	606, 651, 2297
Eco81I	CCTNAGG	1 cut(s)	158
Eco88I	CYCGRG	4 cut(s)	138, 241, 495, 1362
EcoICRI	GAGCTC	3 cut(s)	606, 651, 2297
EcoRI	GAATTC	1 cut(s)	772
EcoRII	CCWGG	2 cut(s)	285, 2494
EcoT14I	CCWWGG	3 cut(s)	1233, 2198, 2244
EcoT38I	GRGCYC	4 cut(s)	233, 608, 653, 2299
EgeI	GGCGCC	1 cut(s)	469
EheI	GGCGCC	1 cut(s)	469
ErhI	CCWWGG	3 cut(s)	1233, 2198, 2244
Esp3I	CGTCTC	1 cut(s)	631
FaeI	CATG	8 cut(s)	91, 554, 1177, 1480, 1745, 1787, 2317, 2431
FaqI	GGGAC	2 cut(s)	728, 2477
FatI	CATG	8 cut(s)	87, 550, 1173, 1476, 1741, 1783, 2313, 2427
FauI	CCCGC	6 cut(s)	83, 136, 156, 206, 353, 1153
FbaI	TGATCA	3 cut(s)	547, 1114, 1600
FblI	GTMKAC	1 cut(s)	291
FriOI	GRGCYC	4 cut(s)	233, 608, 653, 2299
FspBI	CTAG	6 cut(s)	672, 1229, 1463, 1560, 2001, 2093
FspI	TGCGCA	1 cut(s)	1788
GlaI	GCGC	4 cut(s)	185, 202, 469, 1788
GsaI	CCCAGC	2 cut(s)	184, 2351
GsuI	CTGGAG	3 cut(s)	1098, 1960, 2430
HaeII	RGCGCY	1 cut(s)	471
HaeIII	GGCC	2 cut(s)	360, 2157
HgaI	GACGC	1 cut(s)	1323
HhaI	GCGC	4 cut(s)	186, 203, 470, 1789
Hin1I	GRCGYC	2 cut(s)	11, 468
Hin1II	CATG	8 cut(s)	91, 554, 1177, 1480, 1745, 1787, 2317, 2431
Hin6I	GCGC	4 cut(s)	184, 201, 468, 1787
HinP1I	GCGC	4 cut(s)	184, 201, 468, 1787
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	2459
HphI	GGTGA	8 cut(s)	494, 635, 857, 919, 1057, 1293, 1955, 2129
Hpy166II	GTNNAC	4 cut(s)	24, 292, 527, 2311
Hpy8I	GTNNAC	4 cut(s)	24, 292, 527, 2311
Hpy99I	CGWCG	6 cut(s)	13, 16, 296, 299, 476, 664
HpyCH4III	ACNGT	8 cut(s)	380, 1137, 1180, 1261, 1680, 1693, 1759, 2130
HpyCH4IV	ACGT	2 cut(s)	11, 2085
HpyCH4V	TGCA	7 cut(s)	1019, 1265, 1385, 1480, 1902, 1910, 2063
HpyF10VI	GCNNNNNNNGC	9 cut(s)	40, 183, 1379, 1391, 1553, 2163, 2339, 2386, 2401
HpyF3I	CTNAG	2 cut(s)	158, 308
HpySE526I	ACGT	2 cut(s)	11, 2085
Hsp92I	GRCGYC	2 cut(s)	11, 468
Hsp92II	CATG	8 cut(s)	91, 554, 1177, 1480, 1745, 1787, 2317, 2431
HspAI	GCGC	4 cut(s)	184, 201, 468, 1787
KasI	GGCGCC	1 cut(s)	467
Kpn2I	TCCGGA	1 cut(s)	207
KpnI	GGTACC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	547, 1114, 1600
KspI	CCGCGG	1 cut(s)	349
Kzo9I	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
Lsp1109I	GCAGC	7 cut(s)	949, 952, 1003, 1369, 1397, 1661, 2342
LweI	GCATC	2 cut(s)	949, 1147
MaeI	CTAG	6 cut(s)	672, 1229, 1463, 1560, 2001, 2093
MaeII	ACGT	2 cut(s)	11, 2085
MaeIII	GTNAC	5 cut(s)	70, 311, 1915, 2044, 2086
MalI	GATC	9 cut(s)	277, 549, 1116, 1320, 1530, 1602, 1740, 1875, 2268
MbiI	CCGCTC	3 cut(s)	113, 151, 256
MboI	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
MboII	GAAGA	8 cut(s)	136, 743, 962, 1190, 1712, 1787, 1841, 2329
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	1 cut(s)	275
MhlI	GDGCHC	6 cut(s)	233, 608, 653, 1124, 1863, 2299
Mly113I	GGCGCC	1 cut(s)	468
MlyI	GAGTC	6 cut(s)	494, 635, 1057, 1076, 1082, 1384
MmeI	TCCRAC	1 cut(s)	368
MroI	TCCGGA	1 cut(s)	207
MseI	TTAA	5 cut(s)	39, 1752, 1854, 2226, 2484
MslI	CAYNNNNRTG	3 cut(s)	29, 1178, 2102
MspA1I	CMGCKG	5 cut(s)	348, 1016, 1076, 1652, 2117
MspCI	CTTAAG	1 cut(s)	2483
MspR9I	CCNGG	9 cut(s)	139, 140, 223, 287, 362, 519, 744, 1515, 2496
MunI	CAATTG	1 cut(s)	1281
Mva1269I	GAATGC	3 cut(s)	784, 1265, 2109
MvaI	CCWGG	2 cut(s)	287, 2496
MvnI	CGCG	3 cut(s)	201, 348, 1317
MwoI	GCNNNNNNNGC	9 cut(s)	40, 183, 1379, 1391, 1553, 2163, 2339, 2386, 2401
NarI	GGCGCC	1 cut(s)	468
NciI	CCSGG	7 cut(s)	139, 140, 223, 362, 519, 744, 1515
NdeII	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
NlaIII	CATG	8 cut(s)	91, 554, 1177, 1480, 1745, 1787, 2317, 2431
NmeAIII	GCCGAG	2 cut(s)	153, 606
NmuCI	GTSAC	3 cut(s)	311, 1915, 2044
NsbI	TGCGCA	1 cut(s)	1788
NspI	RCATGY	2 cut(s)	1480, 1787
NspV	TTCGAA	1 cut(s)	776
PaeI	GCATGC	1 cut(s)	1480
PagI	TCATGA	2 cut(s)	550, 1741
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	3 cut(s)	784, 1265, 2109
PfeI	GAWTC	6 cut(s)	371, 631, 655, 824, 1149, 1498
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	1 cut(s)	517
PinAI	ACCGGT	1 cut(s)	615
PleI	GAGTC	6 cut(s)	493, 634, 1056, 1076, 1082, 1383
PluTI	GGCGCC	1 cut(s)	471
PpsI	GAGTC	6 cut(s)	493, 634, 1056, 1076, 1082, 1383
Psp124BI	GAGCTC	3 cut(s)	608, 653, 2299
Psp6I	CCWGG	2 cut(s)	285, 2494
PspFI	CCCAGC	2 cut(s)	180, 2347
PspGI	CCWGG	2 cut(s)	285, 2494
PspPI	GGNCC	5 cut(s)	98, 210, 333, 755, 1705
PstI	CTGCAG	1 cut(s)	1387
PsuI	RGATCY	1 cut(s)	275
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	4 cut(s)	1016, 1076, 1652, 2117
RsaI	GTAC	7 cut(s)	126, 164, 989, 1185, 1344, 1450, 2256
RsaNI	GTAC	7 cut(s)	125, 163, 988, 1184, 1343, 1449, 2255
RseI	CAYNNNNRTG	3 cut(s)	29, 1178, 2102
SacI	GAGCTC	3 cut(s)	608, 653, 2299
SacII	CCGCGG	1 cut(s)	349
SalI	GTCGAC	1 cut(s)	290
SaqAI	TTAA	5 cut(s)	39, 1752, 1854, 2226, 2484
Sau3AI	GATC	9 cut(s)	275, 547, 1114, 1318, 1528, 1600, 1738, 1873, 2266
Sau96I	GGNCC	5 cut(s)	98, 210, 333, 755, 1705
SchI	GAGTC	6 cut(s)	494, 635, 1057, 1076, 1082, 1384
ScrFI	CCNGG	9 cut(s)	139, 140, 223, 287, 362, 519, 744, 1515, 2496
SduI	GDGCHC	6 cut(s)	233, 608, 653, 1124, 1863, 2299
SfaNI	GCATC	2 cut(s)	949, 1147
SfcI	CTRYAG	1 cut(s)	1383
SfoI	GGCGCC	1 cut(s)	469
Sfr303I	CCGCGG	1 cut(s)	349
SfuI	TTCGAA	1 cut(s)	776
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	5 cut(s)	98, 210, 333, 755, 1705
SmaI	CCCGGG	1 cut(s)	140
SmiMI	CAYNNNNRTG	3 cut(s)	29, 1178, 2102
SmlI	CTYRAG	1 cut(s)	2483
SmoI	CTYRAG	1 cut(s)	2483
SphI	GCATGC	1 cut(s)	1480
SspDI	GGCGCC	1 cut(s)	467
SspI	AATATT	1 cut(s)	2184
SspMI	CTAG	6 cut(s)	672, 1229, 1463, 1560, 2001, 2093
SstI	GAGCTC	3 cut(s)	608, 653, 2299
StyD4I	CCNGG	9 cut(s)	137, 138, 221, 285, 360, 517, 742, 1513, 2494
StyI	CCWWGG	3 cut(s)	1233, 2198, 2244
TaaI	ACNGT	8 cut(s)	380, 1137, 1180, 1261, 1680, 1693, 1759, 2130
TaiI	ACGT	2 cut(s)	14, 2088
TaqI	TCGA	9 cut(s)	118, 291, 488, 602, 608, 629, 653, 776, 788
TaqII	GACCGA	3 cut(s)	350, 498, 1114
TatI	WGTACW	1 cut(s)	1342
TauI	GCSGC	5 cut(s)	249, 266, 420, 423, 468
TfiI	GAWTC	6 cut(s)	371, 631, 655, 824, 1149, 1498
Tru1I	TTAA	5 cut(s)	39, 1752, 1854, 2226, 2484
Tru9I	TTAA	5 cut(s)	39, 1752, 1854, 2226, 2484
TscAI	CASTG	3 cut(s)	1124, 1140, 1777
TseFI	GTSAC	3 cut(s)	311, 1915, 2044
TseI	GCWGC	7 cut(s)	962, 965, 1016, 1382, 1385, 1649, 2330
Tsp45I	GTSAC	3 cut(s)	311, 1915, 2044
TspDTI	ATGAA	6 cut(s)	17, 104, 1608, 1758, 2028, 2330
TspGWI	ACGGA	5 cut(s)	544, 648, 697, 1613, 1697
TspMI	CCCGGG	1 cut(s)	138
TspRI	CASTG	3 cut(s)	1124, 1140, 1777
Tth111I	GACNNNGTC	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	2483
VpaK11BI	GGWCC	5 cut(s)	98, 210, 333, 755, 1705
XapI	RAATTY	7 cut(s)	92, 707, 760, 772, 1745, 2015, 2207
XbaI	TCTAGA	1 cut(s)	1462
XceI	RCATGY	2 cut(s)	1480, 1787
XmaI	CCCGGG	1 cut(s)	138
XmiI	GTMKAC	1 cut(s)	291
XspI	CTAG	6 cut(s)	672, 1229, 1463, 1560, 2001, 2093
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.