Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Forward (+)

17107415 .. 17112051

Gene Structure 4637 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00297140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGAAAACGACGTCGCTTTCGTCCACTATTATGCCCGTTAAGCTCCTCCTCAACAACCCTTCTCTCCCAGTAACCCGCTTCCTCTCCATGAAATTCGGACCCTCCATTTCCGCTCCTCGACCCGTACCCGCTTTCTCCCGGGTCTTCCCGCTCCGCCTCAGGTACCTCGCCCTCGGCTCCCAGCGCACATTCTCCACCCGCGCCTTCCGGGCCCCACGAACCGGGTCGGGCTCCCAATTCTCGGGCGGCTACGAGCGGAAGGCGGCGGTCGGGGGATCCAAGAGCCTGGTCGACGACGAGGCGGAGCTGAGTGACTGGGTCAGCGAGTTGAGGACCGACCCGACCCGCGGTGGCCGTGGCGGTGGCCGTGGCCGGGGAGAGGAATCGGACGGTGGTGGTCGGAGAGGTAGTAGGGTTAGGGATAGGGGCGGCGATAGAGAGCCGTATCCGGCGAAGCGGAGCAGGAGAGATAGCGGCGCCGACGAGCTTGGTGAGTCGAGGACTCGGGGAGGGTTTCGGTCTTTTCCGGGAAGTTCACGGATGAGTAAAAGGGATGATCATGATGATCGAGAAGGTAGGTCATTTCCGCCGAGAAGCAACCGTGGGAGTTCGAGCTCGAATGACCGGTTTGGTGAGTCGAATCGGAGACGATTTCCGAGCTCGAATCCGTCGGCATTTACTAGGAATCCACGGATGGATAGAAGGTTTGATAACAAATTTGGGGACAATGCTGTTAAGGAAGATGAGTTTCCGGGGAGGAGAGGTCCGAATTTCAGAGGGAATTCGAATGCGAGTTCGAGTTCTTTGAGTAAGAGAGGAGGGAGAGAGGTGGATTCGGGTTTCAGACGAGGCGGCGAGGGGTTGAGGAAGGGTGGGAGAGATTCGAGGAAGGAAAAGGAAATGCTTATGGTGGATGATAGTGATGAGGATGAGGAGGTGAATGCGCTTGGTGTTGGTATTGGGGATTTGCTTACAGAGGAAGATAGTGATAGTGCTGCTTCGGATGAGGATGATGAGTACGAAGGATTGAAAGATAAGAGTGCAGCTGCATTGTTTGGTTCGGATAAGGAAGTGCTGAGTCAGAAGGCTGTGCCGAAAAGTTCAGCGGGAGACTCTGACTCGTATTTGAGTGAGTCAAGGTTTGATCAGTGTTCGGTCTCGCCACTGTCCTTGAAGGGAATCAAGGATGCGGGATATGAGAACATGACTGTGGTACAAGAGGCTACTCTTCCCATCATACTCAAAGGCAAAGATGTTCTAGCCAAGGCTAAAACCGGAACTGGAAAAACTGTTGCATTCTTGCTTCCATCAATTGAAGTTGTTGTAAAATCACCTCCTATTGGACGCGATCATAAGAGACCCCCAATTTCTGTACTTGTAATATGCCCAACCCGAGAGCTGGCGAGTCAAGCTGCAGCAGAAGCCAATAAATTGTTGAAGTATCATCCTTCTATTGGTGTTCAAGTTGTGATAGGAGGTACAAGACTCGCTCTAGAACAGAAACGCATGCAAGCAAACCCTTGCCAGATTCTTGTCGCTACACCGGGAAGGCTCAAAGATCACATTGAGAATACCGCAGGTTTCGCAACTAGGCTGATGGGTGTCAAAGTCCTCATTCTTGATGAAGCTGATCATCTACTGGATATGGGGTTCCGTAAAGACATTGAAAGGATTATAGCAGCTGTTCCAAAACAGCGACAGACACTACTGTTCTCTGCCACTGTTCCAGAAGAGGTCCGTCAAATCTGTCATATCGCTTTGAAAAGAGATCATGAATTTATTAACACGGTTCAGGAAGGCAGTGAAGAGACACATGCGCAGGTCAGACAAACCCATTTGGTTGCTCCTTTGGACAAACACTTTTCCTTTGTATATAGTCTTCTTAAAGAGCACATTGCTGACGATCCTGACTATAAGGTTCTCGTATTCTGCACTACTGCAATGGTCACACGACTGGTTGCTGACCTTCTTGGTGAGCTGAACTTGAATGTTAGAGAAATCCACTCCAGAAAGCCACAGAGTTATAGAACTAGGGTTTCCGATGAATTTCGGAAATCAAGGGGTCTTATCCTTGTGACTTCTGATGTATCTGCACGAGGGGTTGATTATCCAGACGTTACTCTAGTCATACAGGTGGGCATTCCAGCTGATAGACAACAGTATATTCACCGACTTGGTAGAACTGGCCGTAAAGGCAAAGAAGGCCAAGGAATATTGCTACTGGCTCCTTGGGAGGAATTTTTCTTATCCACTATTAAAGATTTGCCAATGACCAAGGCTCCTGTACCTTTAGTTGATCCAGATACCACCAAGAAGGTAGCACGAGCTCTATCCCAAGTGGACATGAAGAACAAAGAAGCAGCATACCAAGCGTGGCTGGGGTATTACAATTCAAATAAGAAAATAGGGCGTGATAAGCATAGGCTTGTGGAGCTTGCTAACGAGTTTAGCCGAAGCATGGGATTGGACAACCCTCCAGCCATTGCCAAGCTTGTACTTGGTAAAATGGGTCTTAAGAATGTCCCTGGACTGCGTTCCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

847

Amino Acids

93.33

Weight (kDa)

9.59

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	404 - 581	2.2e-45	DEAD/DEAH box helicase
ResIII	PF04851	415 - 576	6e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	627 - 732	5.9e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	1818
Acc36I	ACCTGC	2 cut(s)	1568, 1810
Acc65I	GGTACC	1 cut(s)	162
AccB1I	GGYRCC	2 cut(s)	162, 476
AccBSI	CCGCTC	3 cut(s)	113, 151, 256
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	3 cut(s)	201, 348, 1347
AclWI	GGATC	4 cut(s)	270, 283, 1898, 2291
AcoI	YGGCCR	4 cut(s)	352, 364, 370, 2185
AcsI	RAATTY	7 cut(s)	92, 716, 769, 781, 1775, 2045, 2237
AcyI	GRCGYC	2 cut(s)	11, 477
AfaI	GTAC	8 cut(s)	126, 164, 1019, 1215, 1374, 1480, 2286, 2496
AfiI	CCNNNNNNNGG	9 cut(s)	221, 241, 347, 373, 379, 1340, 1399, 1454, 2458
AflII	CTTAAG	1 cut(s)	2513
AgeI	ACCGGT	1 cut(s)	624
AgsI	TTSAA	9 cut(s)	1030, 1174, 1316, 1438, 1463, 1667, 1762, 1987, 2394
AjnI	CCWGG	2 cut(s)	285, 2524
AloI	GAACNNNNNNTCC	2 cut(s)	1634, 1666
Alw21I	GWGCWC	4 cut(s)	617, 662, 1893, 2329
Alw26I	GTCTC	5 cut(s)	640, 1104, 1162, 1351, 1802
AlwI	GGATC	4 cut(s)	270, 283, 1898, 2291
Ama87I	CYCGRG	4 cut(s)	138, 241, 504, 1392
AoxI	GGCC	6 cut(s)	210, 352, 364, 370, 2185, 2203
ApaI	GGGCCC	1 cut(s)	214
ApeKI	GCWGC	7 cut(s)	995, 1043, 1046, 1412, 1415, 1679, 2360
ApoI	RAATTY	7 cut(s)	92, 716, 769, 781, 1775, 2045, 2237
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AsiGI	ACCGGT	1 cut(s)	624
Asp718I	GGTACC	1 cut(s)	162
AspLEI	GCGC	5 cut(s)	186, 203, 479, 946, 1819
AspS9I	GGNCC	6 cut(s)	98, 210, 211, 333, 764, 1735
AsuC2I	CCSGG	8 cut(s)	139, 140, 209, 223, 374, 528, 753, 1545
AsuHPI	GGTGA	6 cut(s)	503, 644, 949, 1323, 1985, 2159
AsuII	TTCGAA	1 cut(s)	785
AvaI	CYCGRG	4 cut(s)	138, 241, 504, 1392
AvaII	GGWCC	4 cut(s)	98, 333, 764, 1735
AxyI	CCTNAGG	1 cut(s)	158
BaeGI	GKGCMC	1 cut(s)	214
BamHI	GGATCC	1 cut(s)	275
BanI	GGYRCC	2 cut(s)	162, 476
BanII	GRGCYC	5 cut(s)	214, 233, 617, 662, 2329
BarI	GAAGNNNNNNTAC	2 cut(s)	1863, 1895
BauI	CACGAG	2 cut(s)	2094, 2322
BbsI	GAAGAC	2 cut(s)	136, 1871
Bbv12I	GWGCWC	4 cut(s)	617, 662, 1893, 2329
BbvI	GCAGC	7 cut(s)	982, 1033, 1055, 1399, 1427, 1691, 2372
BccI	CCATC	4 cut(s)	688, 1241, 1315, 1591
BceAI	ACGGC	4 cut(s)	339, 351, 427, 2172
BciT130I	CCWGG	2 cut(s)	287, 2526
BciVI	GTATCC	1 cut(s)	456
BclI	TGATCA	3 cut(s)	556, 1144, 1630
BcnI	CCSGG	8 cut(s)	139, 140, 209, 223, 374, 528, 753, 1545
BcoDI	GTCTC	5 cut(s)	640, 1104, 1162, 1351, 1802
BfaI	CTAG	6 cut(s)	681, 1259, 1493, 1590, 2031, 2123
BfmI	CTRYAG	1 cut(s)	1413
BfoI	RGCGCY	1 cut(s)	480
BfrI	CTTAAG	1 cut(s)	2513
BfuAI	ACCTGC	2 cut(s)	1568, 1810
BfuI	GTATCC	1 cut(s)	456
BglI	GCCNNNNNGGC	2 cut(s)	209, 2193
Bme18I	GGWCC	4 cut(s)	98, 333, 764, 1735
BmeT110I	CYCGRG	4 cut(s)	138, 241, 504, 1392
BmgT120I	GGNCC	6 cut(s)	98, 210, 211, 333, 764, 1735
BmrI	ACTGGG	2 cut(s)	62, 325
BmsI	GCATC	1 cut(s)	1177
BmuI	ACTGGG	2 cut(s)	62, 325
BpiI	GAAGAC	2 cut(s)	136, 1871
BpmI	CTGGAG	2 cut(s)	1990, 2460
Bpu14I	TTCGAA	1 cut(s)	785
BpuMI	CCSGG	8 cut(s)	139, 140, 209, 223, 374, 528, 753, 1545
BsaHI	GRCGYC	2 cut(s)	11, 477
BsaI	GGTCTC	2 cut(s)	1162, 1351
BsaWI	WCCGGW	2 cut(s)	624, 1274
BsaXI	ACNNNNNCTCC	6 cut(s)	394, 424, 748, 778, 2263, 2293
Bsc4I	CCNNNNNNNGG	9 cut(s)	221, 241, 347, 373, 379, 1340, 1399, 1454, 2458
Bse118I	RCCGGY	1 cut(s)	624
Bse1I	ACTGG	7 cut(s)	68, 320, 1285, 1644, 1959, 2188, 2226
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	3 cut(s)	1893, 1947, 2481
BseBI	CCWGG	2 cut(s)	287, 2526
BseGI	GGATG	9 cut(s)	546, 559, 699, 919, 934, 1009, 1015, 1192, 1444
BseLI	CCNNNNNNNGG	9 cut(s)	221, 241, 347, 373, 379, 1340, 1399, 1454, 2458
BseMI	GCAATG	3 cut(s)	1893, 1947, 2481
BseMII	CTCAG	3 cut(s)	172, 299, 1067
BseNI	ACTGG	7 cut(s)	68, 320, 1285, 1644, 1959, 2188, 2226
BseRI	GAGGAG	6 cut(s)	35, 38, 105, 772, 831, 947
BseSI	GKGCMC	1 cut(s)	214
BseXI	GCAGC	7 cut(s)	982, 1033, 1055, 1399, 1427, 1691, 2372
BseYI	CCCAGC	2 cut(s)	180, 2377
BsgI	GTGCAG	3 cut(s)	1062, 1915, 2076
Bsh1236I	CGCG	3 cut(s)	201, 348, 1347
Bsh1285I	CGRYCG	1 cut(s)	270
BshFI	GGCC	6 cut(s)	212, 354, 366, 372, 2187, 2205
BshNI	GGYRCC	2 cut(s)	162, 476
BshTI	ACCGGT	1 cut(s)	624
BsiEI	CGRYCG	1 cut(s)	270
BsiHKAI	GWGCWC	4 cut(s)	617, 662, 1893, 2329
BsiHKCI	CYCGRG	4 cut(s)	138, 241, 504, 1392
BslFI	GGGAC	2 cut(s)	737, 2507
BslI	CCNNNNNNNGG	9 cut(s)	221, 241, 347, 373, 379, 1340, 1399, 1454, 2458
BsmAI	GTCTC	5 cut(s)	640, 1104, 1162, 1351, 1802
BsmBI	CGTCTC	1 cut(s)	640
BsmFI	GGGAC	2 cut(s)	737, 2507
BsmI	GAATGC	4 cut(s)	793, 946, 1295, 2139
BsnI	GGCC	6 cut(s)	212, 354, 366, 372, 2187, 2205
Bso31I	GGTCTC	2 cut(s)	1162, 1351
BsoBI	CYCGRG	4 cut(s)	138, 241, 504, 1392
Bsp119I	TTCGAA	1 cut(s)	785
Bsp120I	GGGCCC	1 cut(s)	210
Bsp1286I	GDGCHC	6 cut(s)	214, 233, 617, 662, 1893, 2329
BspANI	GGCC	6 cut(s)	212, 354, 366, 372, 2187, 2205
BspCNI	CTCAG	3 cut(s)	171, 300, 1068
BspFNI	CGCG	3 cut(s)	201, 348, 1347
BspHI	TCATGA	2 cut(s)	559, 1771
BspMAI	CTGCAG	1 cut(s)	1417
BspMI	ACCTGC	2 cut(s)	1568, 1810
BspPI	GGATC	4 cut(s)	270, 283, 1898, 2291
BspT104I	TTCGAA	1 cut(s)	785
BspT107I	GGYRCC	2 cut(s)	162, 476
BspTI	CTTAAG	1 cut(s)	2513
BspTNI	GGTCTC	2 cut(s)	1162, 1351
BsrBI	CCGCTC	3 cut(s)	113, 151, 256
BsrDI	GCAATG	3 cut(s)	1893, 1947, 2481
BsrFI	RCCGGY	1 cut(s)	624
BsrI	ACTGG	7 cut(s)	68, 320, 1285, 1644, 1959, 2188, 2226
BssAI	RCCGGY	1 cut(s)	624
BssNI	GRCGYC	2 cut(s)	11, 477
BssSI	CACGAG	2 cut(s)	2094, 2322
BssT1I	CCWWGG	4 cut(s)	1263, 2206, 2228, 2274
Bst2BI	CACGAG	2 cut(s)	2094, 2322
Bst2UI	CCWGG	2 cut(s)	287, 2526
Bst4CI	ACNGT	9 cut(s)	392, 602, 1167, 1210, 1291, 1710, 1723, 1789, 2160
Bst6I	CTCTTC	3 cut(s)	1233, 1725, 1800
BstACI	GRCGYC	2 cut(s)	11, 477
BstAFI	CTTAAG	1 cut(s)	2513
BstBI	TTCGAA	1 cut(s)	785
BstC8I	GCNNGC	4 cut(s)	1401, 1508, 1512, 2436
BstDEI	CTNAG	3 cut(s)	158, 308, 1076
BstDSI	CCRYGG	5 cut(s)	346, 355, 367, 601, 689
BstF5I	GGATG	9 cut(s)	546, 559, 699, 919, 934, 1009, 1015, 1192, 1444
BstFNI	CGCG	3 cut(s)	201, 348, 1347
BstH2I	RGCGCY	1 cut(s)	480
BstHHI	GCGC	5 cut(s)	186, 203, 479, 946, 1819
BstMAI	GTCTC	5 cut(s)	640, 1104, 1162, 1351, 1802
BstMCI	CGRYCG	1 cut(s)	270
BstNI	CCWGG	2 cut(s)	287, 2526
BstNSI	RCATGY	2 cut(s)	1510, 1817
BstSFI	CTRYAG	1 cut(s)	1413
BstSLI	GKGCMC	1 cut(s)	214
BstUI	CGCG	3 cut(s)	201, 348, 1347
BstV1I	GCAGC	7 cut(s)	982, 1033, 1055, 1399, 1427, 1691, 2372
BstV2I	GAAGAC	2 cut(s)	136, 1871
BstX2I	RGATCY	1 cut(s)	275
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	275
Bsu36I	CCTNAGG	1 cut(s)	158
BsuI	GTATCC	1 cut(s)	456
BsuRI	GGCC	6 cut(s)	212, 354, 366, 372, 2187, 2205
BtgI	CCRYGG	5 cut(s)	346, 355, 367, 601, 689
BtsCI	GGATG	9 cut(s)	546, 559, 699, 919, 934, 1009, 1015, 1192, 1444
BtsI	GCAGTG	1 cut(s)	1807
BtsIMutI	CAGTG	4 cut(s)	1154, 1163, 1719, 1807
BveI	ACCTGC	2 cut(s)	1568, 1810
Cac8I	GCNNGC	4 cut(s)	1401, 1508, 1512, 2436
CciI	TCATGA	2 cut(s)	559, 1771
CfoI	GCGC	5 cut(s)	186, 203, 479, 946, 1819
Cfr10I	RCCGGY	1 cut(s)	624
Cfr13I	GGNCC	6 cut(s)	98, 210, 211, 333, 764, 1735
Cfr42I	CCGCGG	1 cut(s)	349
Cfr9I	CCCGGG	1 cut(s)	138
CseI	GACGC	1 cut(s)	1353
Csp6I	GTAC	8 cut(s)	125, 163, 1018, 1214, 1373, 1479, 2285, 2495
CspAI	ACCGGT	1 cut(s)	624
CspCI	CAANNNNNGTGG	2 cut(s)	1152, 1187
CviAII	CATG	8 cut(s)	88, 560, 1204, 1507, 1772, 1814, 2344, 2458
CviQI	GTAC	8 cut(s)	125, 163, 1018, 1214, 1373, 1479, 2285, 2495
DdeI	CTNAG	3 cut(s)	158, 308, 1076
DinI	GGCGCC	1 cut(s)	478
EaeI	YGGCCR	4 cut(s)	352, 364, 370, 2185
Eam1104I	CTCTTC	3 cut(s)	1233, 1725, 1800
EarI	CTCTTC	3 cut(s)	1233, 1725, 1800
EciI	GGCGGA	3 cut(s)	143, 317, 576
Ecl136II	GAGCTC	3 cut(s)	615, 660, 2327
Eco130I	CCWWGG	4 cut(s)	1263, 2206, 2228, 2274
Eco24I	GRGCYC	5 cut(s)	214, 233, 617, 662, 2329
Eco31I	GGTCTC	2 cut(s)	1162, 1351
Eco47I	GGWCC	4 cut(s)	98, 333, 764, 1735
Eco53kI	GAGCTC	3 cut(s)	615, 660, 2327
Eco81I	CCTNAGG	1 cut(s)	158
Eco88I	CYCGRG	4 cut(s)	138, 241, 504, 1392
EcoICRI	GAGCTC	3 cut(s)	615, 660, 2327
EcoO109I	RGGNCCY	1 cut(s)	211
EcoRI	GAATTC	1 cut(s)	781
EcoRII	CCWGG	2 cut(s)	285, 2524
EcoT14I	CCWWGG	4 cut(s)	1263, 2206, 2228, 2274
EcoT38I	GRGCYC	5 cut(s)	214, 233, 617, 662, 2329
EgeI	GGCGCC	1 cut(s)	478
EheI	GGCGCC	1 cut(s)	478
ErhI	CCWWGG	4 cut(s)	1263, 2206, 2228, 2274
Esp3I	CGTCTC	1 cut(s)	640
FaeI	CATG	8 cut(s)	91, 563, 1207, 1510, 1775, 1817, 2347, 2461
FaqI	GGGAC	2 cut(s)	737, 2507
FatI	CATG	8 cut(s)	87, 559, 1203, 1506, 1771, 1813, 2343, 2457
FauI	CCCGC	7 cut(s)	83, 136, 156, 206, 353, 1099, 1183
FbaI	TGATCA	3 cut(s)	556, 1144, 1630
FblI	GTMKAC	1 cut(s)	291
FokI	GGATG	9 cut(s)	553, 566, 706, 926, 941, 1016, 1022, 1199, 1431
FriOI	GRGCYC	5 cut(s)	214, 233, 617, 662, 2329
FspBI	CTAG	6 cut(s)	681, 1259, 1493, 1590, 2031, 2123
FspI	TGCGCA	1 cut(s)	1818
GlaI	GCGC	5 cut(s)	185, 202, 478, 945, 1818
GsaI	CCCAGC	2 cut(s)	184, 2381
GsuI	CTGGAG	2 cut(s)	1990, 2460
HaeII	RGCGCY	1 cut(s)	480
HaeIII	GGCC	6 cut(s)	212, 354, 366, 372, 2187, 2205
HgaI	GACGC	1 cut(s)	1353
HhaI	GCGC	5 cut(s)	186, 203, 479, 946, 1819
Hin1I	GRCGYC	2 cut(s)	11, 477
Hin1II	CATG	8 cut(s)	91, 563, 1207, 1510, 1775, 1817, 2347, 2461
Hin6I	GCGC	5 cut(s)	184, 201, 477, 944, 1817
HinP1I	GCGC	5 cut(s)	184, 201, 477, 944, 1817
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	2489
HphI	GGTGA	6 cut(s)	503, 644, 949, 1323, 1985, 2159
Hpy166II	GTNNAC	4 cut(s)	24, 292, 536, 2341
Hpy8I	GTNNAC	4 cut(s)	24, 292, 536, 2341
Hpy99I	CGWCG	6 cut(s)	13, 16, 296, 299, 485, 673
HpyCH4III	ACNGT	9 cut(s)	392, 602, 1167, 1210, 1291, 1710, 1723, 1789, 2160
HpyCH4IV	ACGT	2 cut(s)	11, 2115
HpyCH4V	TGCA	8 cut(s)	1043, 1049, 1295, 1415, 1510, 1932, 1940, 2093
HpyF3I	CTNAG	3 cut(s)	158, 308, 1076
HpySE526I	ACGT	2 cut(s)	11, 2115
Hsp92I	GRCGYC	2 cut(s)	11, 477
Hsp92II	CATG	8 cut(s)	91, 563, 1207, 1510, 1775, 1817, 2347, 2461
HspAI	GCGC	5 cut(s)	184, 201, 477, 944, 1817
KasI	GGCGCC	1 cut(s)	476
KpnI	GGTACC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	556, 1144, 1630
KspI	CCGCGG	1 cut(s)	349
Lsp1109I	GCAGC	7 cut(s)	982, 1033, 1055, 1399, 1427, 1691, 2372
LweI	GCATC	1 cut(s)	1177
MaeI	CTAG	6 cut(s)	681, 1259, 1493, 1590, 2031, 2123
MaeII	ACGT	2 cut(s)	11, 2115
MaeIII	GTNAC	5 cut(s)	70, 311, 1945, 2074, 2116
MbiI	CCGCTC	3 cut(s)	113, 151, 256
MboII	GAAGA	8 cut(s)	136, 752, 992, 1220, 1742, 1817, 1871, 2359
MfeI	CAATTG	1 cut(s)	1311
MflI	RGATCY	1 cut(s)	275
MhlI	GDGCHC	6 cut(s)	214, 233, 617, 662, 1893, 2329
Mly113I	GGCGCC	1 cut(s)	477
MlyI	GAGTC	9 cut(s)	496, 503, 644, 1087, 1106, 1112, 1142, 1414, 1479
MmeI	TCCRAC	1 cut(s)	380
MseI	TTAA	6 cut(s)	39, 735, 1782, 1884, 2256, 2514
MslI	CAYNNNNRTG	3 cut(s)	29, 1208, 2132
MspA1I	CMGCKG	5 cut(s)	348, 1046, 1106, 1682, 2147
MspCI	CTTAAG	1 cut(s)	2513
MunI	CAATTG	1 cut(s)	1311
Mva1269I	GAATGC	4 cut(s)	793, 946, 1295, 2139
MvaI	CCWGG	2 cut(s)	287, 2526
MvnI	CGCG	3 cut(s)	201, 348, 1347
NarI	GGCGCC	1 cut(s)	477
NciI	CCSGG	8 cut(s)	139, 140, 209, 223, 374, 528, 753, 1545
NlaIII	CATG	8 cut(s)	91, 563, 1207, 1510, 1775, 1817, 2347, 2461
NmeAIII	GCCGAG	2 cut(s)	153, 615
NmuCI	GTSAC	3 cut(s)	311, 1945, 2074
NsbI	TGCGCA	1 cut(s)	1818
NspI	RCATGY	2 cut(s)	1510, 1817
NspV	TTCGAA	1 cut(s)	785
PaeI	GCATGC	1 cut(s)	1510
PagI	TCATGA	2 cut(s)	559, 1771
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	4 cut(s)	793, 946, 1295, 2139
PfeI	GAWTC	8 cut(s)	383, 640, 664, 685, 833, 881, 1179, 1528
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	1 cut(s)	526
PinAI	ACCGGT	1 cut(s)	624
PleI	GAGTC	9 cut(s)	496, 502, 643, 1086, 1106, 1112, 1141, 1413, 1479
PluTI	GGCGCC	1 cut(s)	480
PpsI	GAGTC	9 cut(s)	496, 502, 643, 1086, 1106, 1112, 1141, 1413, 1479
Psp124BI	GAGCTC	3 cut(s)	617, 662, 2329
Psp6I	CCWGG	2 cut(s)	285, 2524
PspFI	CCCAGC	2 cut(s)	180, 2377
PspGI	CCWGG	2 cut(s)	285, 2524
PspOMI	GGGCCC	1 cut(s)	210
PspPI	GGNCC	6 cut(s)	98, 210, 211, 333, 764, 1735
PstI	CTGCAG	1 cut(s)	1417
PsuI	RGATCY	1 cut(s)	275
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	3 cut(s)	1046, 1682, 2147
RsaI	GTAC	8 cut(s)	126, 164, 1019, 1215, 1374, 1480, 2286, 2496
RsaNI	GTAC	8 cut(s)	125, 163, 1018, 1214, 1373, 1479, 2285, 2495
RseI	CAYNNNNRTG	3 cut(s)	29, 1208, 2132
SacI	GAGCTC	3 cut(s)	617, 662, 2329
SacII	CCGCGG	1 cut(s)	349
SalI	GTCGAC	1 cut(s)	290
SaqAI	TTAA	6 cut(s)	39, 735, 1782, 1884, 2256, 2514
Sau96I	GGNCC	6 cut(s)	98, 210, 211, 333, 764, 1735
SchI	GAGTC	9 cut(s)	496, 503, 644, 1087, 1106, 1112, 1142, 1414, 1479
SduI	GDGCHC	6 cut(s)	214, 233, 617, 662, 1893, 2329
SfaNI	GCATC	1 cut(s)	1177
SfcI	CTRYAG	1 cut(s)	1413
SfoI	GGCGCC	1 cut(s)	478
Sfr303I	CCGCGG	1 cut(s)	349
SfuI	TTCGAA	1 cut(s)	785
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	4 cut(s)	98, 333, 764, 1735
SmaI	CCCGGG	1 cut(s)	140
SmiMI	CAYNNNNRTG	3 cut(s)	29, 1208, 2132
SmlI	CTYRAG	1 cut(s)	2513
SmoI	CTYRAG	1 cut(s)	2513
SphI	GCATGC	1 cut(s)	1510
SspDI	GGCGCC	1 cut(s)	476
SspI	AATATT	1 cut(s)	2214
SspMI	CTAG	6 cut(s)	681, 1259, 1493, 1590, 2031, 2123
SstI	GAGCTC	3 cut(s)	617, 662, 2329
StyI	CCWWGG	4 cut(s)	1263, 2206, 2228, 2274
TaaI	ACNGT	9 cut(s)	392, 602, 1167, 1210, 1291, 1710, 1723, 1789, 2160
TaiI	ACGT	2 cut(s)	14, 2118
TaqII	GACCGA	3 cut(s)	350, 507, 1144
TatI	WGTACW	2 cut(s)	1372, 2494
TauI	GCSGC	5 cut(s)	249, 266, 432, 477, 855
TfiI	GAWTC	8 cut(s)	383, 640, 664, 685, 833, 881, 1179, 1528
Tru1I	TTAA	6 cut(s)	39, 735, 1782, 1884, 2256, 2514
Tru9I	TTAA	6 cut(s)	39, 735, 1782, 1884, 2256, 2514
TscAI	CASTG	4 cut(s)	1154, 1170, 1726, 1807
TseFI	GTSAC	3 cut(s)	311, 1945, 2074
TseI	GCWGC	7 cut(s)	995, 1043, 1046, 1412, 1415, 1679, 2360
Tsp45I	GTSAC	3 cut(s)	311, 1945, 2074
TspDTI	ATGAA	6 cut(s)	17, 104, 1638, 1788, 2058, 2360
TspGWI	ACGGA	5 cut(s)	553, 657, 706, 1643, 1727
TspMI	CCCGGG	1 cut(s)	138
TspRI	CASTG	4 cut(s)	1154, 1170, 1726, 1807
Tth111I	GACNNNGTC	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	2513
VpaK11BI	GGWCC	4 cut(s)	98, 333, 764, 1735
XapI	RAATTY	7 cut(s)	92, 716, 769, 781, 1775, 2045, 2237
XbaI	TCTAGA	1 cut(s)	1492
XceI	RCATGY	2 cut(s)	1510, 1817
XmaI	CCCGGG	1 cut(s)	138
XmiI	GTMKAC	1 cut(s)	291
XspI	CTAG	6 cut(s)	681, 1259, 1493, 1590, 2031, 2123
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.