MD01G1086200.v1.1

Protein of unknown function (DUF4079)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011218
Physical Location & Seq
Forward (+)
19273402 .. 19275761
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1086200.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCAGCCACACTCAGCCTCCTCAGACTCCCCACGCTTCCCCAAAAGCCCACTCATTGTCTCTCAAAAATCCAACGAAGTTCATCAATCCCAACAAAACCCAGCAATGGCAAGGACTCAACTAAAGTCCTCATAACCACCATCGACCGCCTTAAGCCAACTTCTCTCCCTCTCACAGCTCTCACATTGCCATTTTTGCTGGACACCAAGGATGCACTTGCTGCAGGTGGGGAGTTTGGGATATTAGAGGGAAGGACATTTGCTCTGATACACCCAATTGTGATGGGAGGGCTGTTTGTGTACACATTGTGGGCAGGTTACTTGGGTTGGCAGTGGAGGAGAGTGAGGACAATCCAGGAAGAAATTAATGAGCTCAAGAAGCAGGTGAAGCCTACCCCTGTCACACCTGAGGGGACTCCAGTGGAAGCTCCCCCATCCCCTTTTGCACTCAAAATTCAGCAGCTTACTGAGGAAAGGAAGGAGTTGGTGAAAGGATCTTACCGGGATAAACATTACAATGCAGGATCAATATTGTTAGCTTTCGGGGTTTTTGAGTCAGTTGGTGGAGGACTTAACACATGGTTGAGGACAGGAAAGCTATTTCCAGGTCCCCATTTGTTTGCCGGGGCAGCTATCACGGTGCTATGGGCAGCAGCTGCAGCTCTTACGCCTGCAATGCAAAAGGGAAGTGAGACAGCCAGAAACCTGCACATTGCGCTGAATGTGATAAACGTTCTTCTTTTCGTGTGGCAAATACCTACTGGATGGGAGATAGTTCTGAAAGTGTTCGAGTTCACTAAATGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4079

Protein Analysis

270

Amino Acids

29.58

Weight (kDa)

9.76

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4079 PF13301 88 - 262 1.2e-46 Protein of unknown function (DUF4079)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61870 AT3G61870
fragaria_vesca FvH4_7g17240 FvH4_7g17240 FvH4_7g17240
malus_domestica MD01G1086200.v1.1 MD07G1155100.v1.1
prunus_persica Prupe.2G193800_v2.0.a1
pyrus_communis pycom07g15230
rosa_chinensis RchiOBHm_Chr1g0360621
rosa_laevigata RLG00000027794
rosa_multiflora Rmu_sc0007110.1_g000006
rosa_roxburghii Rroxscaffold_4G00295950
rosa_rugosa Rorug01G0280300.1 Rorug01G0280400
rosa_samantha Rh1AG292300 Rh1BG257200 Rh1CG275000 Rh1DG287100
rosa_wichuraiana Rw1G025880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 215, 373
Acc36I ACCTGC 4 cut(s) 215, 305, 373, 714
AciI CCGC 1 cut(s) 148
AclI AACGTT 1 cut(s) 732
AclWI GGATC 2 cut(s) 502, 532
AcsI RAATTY 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 1 cut(s) 107
AflII CTTAAG 1 cut(s) 152
AjnI CCWGG 2 cut(s) 354, 604
AluBI AGCT 9 cut(s) 179, 373, 428, 463, 539, 598, 632, 656, 662
AluI AGCT 9 cut(s) 179, 373, 428, 463, 539, 598, 632, 656, 662
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 2 cut(s) 65, 686
AlwI GGATC 2 cut(s) 502, 532
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 1 cut(s) 803
ApeKI GCWGC 8 cut(s) 5, 221, 460, 629, 650, 653, 656, 659
ApoI RAATTY 1 cut(s) 453
AseI ATTAAT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 1 cut(s) 718
AspS9I GGNCC 1 cut(s) 608
AsuC2I CCSGG 2 cut(s) 503, 625
AsuHPI GGTGA 2 cut(s) 397, 499
AvaII GGWCC 1 cut(s) 608
AxyI CCTNAGG 1 cut(s) 408
BanII GRGCYC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 8 cut(s) 17, 208, 472, 641, 643, 662, 665, 671
BccI CCATC 4 cut(s) 149, 277, 442, 759
BciT130I CCWGG 2 cut(s) 356, 606
BcnI CCSGG 2 cut(s) 503, 625
BcoDI GTCTC 2 cut(s) 65, 686
BfmI CTRYAG 2 cut(s) 222, 657
BfrI CTTAAG 1 cut(s) 152
BfuAI ACCTGC 4 cut(s) 215, 305, 373, 714
BisI GCNGC 8 cut(s) 6, 222, 461, 630, 651, 654, 657, 660
BlsI GCNGC 8 cut(s) 7, 223, 462, 631, 652, 655, 658, 661
Bme1390I CCNGG 4 cut(s) 356, 503, 606, 625
Bme18I GGWCC 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 608
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 4 cut(s) 356, 503, 606, 625
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 503, 625
BsaJI CCNNGG 2 cut(s) 207, 624
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 419, 766
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 4 cut(s) 112, 185, 681, 711
BseBI CCWGG 2 cut(s) 356, 606
BseDI CCNNGG 2 cut(s) 207, 624
BseGI GGATG 3 cut(s) 217, 434, 770
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMI GCAATG 4 cut(s) 112, 185, 681, 711
BseMII CTCAG 4 cut(s) 28, 37, 399, 459
BseNI ACTGG 2 cut(s) 419, 766
BseRI GAGGAG 2 cut(s) 11, 352
BseXI GCAGC 8 cut(s) 17, 208, 472, 641, 643, 662, 665, 671
BseYI CCCAGC 1 cut(s) 101
BsgI GTGCAG 1 cut(s) 692
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 1 cut(s) 805
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 2 cut(s) 502, 624
BslFI GGGAC 2 cut(s) 427, 594
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 65, 686
BsmFI GGGAC 2 cut(s) 427, 594
BsnI GGCC 1 cut(s) 805
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 494, 524
BspACI CCGC 1 cut(s) 148
BspANI GGCC 1 cut(s) 805
BspCNI CTCAG 4 cut(s) 27, 36, 400, 460
BspLI GGNNCC 1 cut(s) 610
BspMAI CTGCAG 2 cut(s) 226, 661
BspMI ACCTGC 4 cut(s) 215, 305, 373, 714
BspPI GGATC 2 cut(s) 502, 532
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 4 cut(s) 112, 185, 681, 711
BsrGI TGTACA 1 cut(s) 300
BsrI ACTGG 2 cut(s) 419, 766
BssECI CCNNGG 2 cut(s) 207, 624
BssMI GATC 2 cut(s) 494, 524
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 356, 606
Bst4CI ACNGT 1 cut(s) 640
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 2 cut(s) 221, 656
BstAUI TGTACA 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 672
BstDEI CTNAG 4 cut(s) 14, 23, 408, 468
BstF5I GGATG 3 cut(s) 217, 434, 770
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 2 cut(s) 497, 527
BstMAI GTCTC 2 cut(s) 65, 686
BstMBI GATC 2 cut(s) 494, 524
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 9 cut(s) 196, 221, 379, 388, 629, 656, 659, 676, 715
BstNI CCWGG 2 cut(s) 356, 606
BstSCI CCNGG 4 cut(s) 354, 501, 604, 623
BstSFI CTRYAG 2 cut(s) 222, 657
BstV1I GCAGC 8 cut(s) 17, 208, 472, 641, 643, 662, 665, 671
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 1 cut(s) 805
BtsCI GGATG 3 cut(s) 217, 434, 770
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 2 cut(s) 338, 426
BveI ACCTGC 4 cut(s) 215, 305, 373, 714
Cac8I GCNNGC 1 cut(s) 672
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 1 cut(s) 718
Cfr13I GGNCC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 301
CviAII CATG 1 cut(s) 579
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 4 cut(s) 14, 23, 408, 468
DpnI GATC 2 cut(s) 496, 526
DpnII GATC 2 cut(s) 494, 524
DraIII CACNNNGTG 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 354, 604
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 582
FaiI YATR 3 cut(s) 134, 580, 646
FaqI GGGAC 2 cut(s) 427, 594
FatI CATG 1 cut(s) 578
Fnu4HI GCNGC 8 cut(s) 6, 222, 461, 630, 651, 654, 657, 660
FokI GGATG 3 cut(s) 224, 421, 777
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 8 cut(s) 6, 222, 461, 630, 651, 654, 657, 660
GlaI GCGC 1 cut(s) 717
GluI GCNGC 8 cut(s) 6, 222, 461, 630, 651, 654, 657, 660
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 805
HapII CCGG 2 cut(s) 502, 624
HhaI GCGC 1 cut(s) 718
Hin1II CATG 1 cut(s) 582
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HinfI GANTC 4 cut(s) 27, 116, 415, 554
HpaII CCGG 2 cut(s) 502, 624
HphI GGTGA 2 cut(s) 397, 499
Hpy166II GTNNAC 3 cut(s) 301, 303, 795
Hpy188I TCNGA 3 cut(s) 26, 267, 780
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 3 cut(s) 301, 303, 795
HpyAV CCTTC 2 cut(s) 246, 472
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 1 cut(s) 732
HpyCH4V TGCA 8 cut(s) 215, 224, 446, 521, 659, 674, 679, 709
HpyF10VI GCNNNNNNNGC 9 cut(s) 196, 221, 379, 388, 629, 656, 659, 676, 715
HpyF3I CTNAG 4 cut(s) 14, 23, 408, 468
HpySE526I ACGT 1 cut(s) 732
Hsp92II CATG 1 cut(s) 582
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 2 cut(s) 494, 524
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 8 cut(s) 17, 208, 472, 641, 643, 662, 665, 671
LweI GCATC 1 cut(s) 202
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 2 cut(s) 317, 400
MalI GATC 2 cut(s) 496, 526
MboI GATC 2 cut(s) 494, 524
MboII GAAGA 2 cut(s) 371, 728
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 3 cut(s) 276, 363, 453
MlyI GAGTC 4 cut(s) 21, 110, 409, 563
MmeI TCCRAC 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 785
MseI TTAA 3 cut(s) 153, 366, 573
MslI CAYNNNNRTG 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 656
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 2 cut(s) 502, 624
MspR9I CCNGG 4 cut(s) 356, 503, 606, 625
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 2 cut(s) 356, 606
MwoI GCNNNNNNNGC 9 cut(s) 196, 221, 379, 388, 629, 656, 659, 676, 715
NciI CCSGG 2 cut(s) 503, 625
NdeII GATC 2 cut(s) 494, 524
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 1 cut(s) 610
NmuCI GTSAC 1 cut(s) 400
PaqCI CACCTGC 2 cut(s) 215, 373
PdmI GAANNNNTTC 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 8 cut(s) 7, 223, 462, 631, 652, 655, 658, 661
PleI GAGTC 4 cut(s) 21, 110, 409, 562
PpsI GAGTC 4 cut(s) 21, 110, 409, 562
PpuMI RGGWCCY 1 cut(s) 608
PshBI ATTAAT 1 cut(s) 366
Psp124BI GAGCTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 732
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 354, 604
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 2 cut(s) 354, 604
PspN4I GGNNCC 1 cut(s) 610
PspPI GGNCC 1 cut(s) 608
PspPPI RGGWCCY 1 cut(s) 608
PstI CTGCAG 2 cut(s) 226, 661
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 494
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 516
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 153, 366, 573
SatI GCNGC 8 cut(s) 6, 222, 461, 630, 651, 654, 657, 660
Sau3AI GATC 2 cut(s) 494, 524
Sau96I GGNCC 1 cut(s) 608
SchI GAGTC 4 cut(s) 21, 110, 409, 563
ScrFI CCNGG 4 cut(s) 356, 503, 606, 625
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 2 cut(s) 222, 657
SinI GGWCC 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 2 cut(s) 152, 374
SmoI CTYRAG 2 cut(s) 152, 374
Sse9I AATT 3 cut(s) 276, 363, 453
SsiI CCGC 1 cut(s) 148
SspI AATATT 1 cut(s) 531
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 4 cut(s) 354, 501, 604, 623
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 1 cut(s) 735
TaqI TCGA 2 cut(s) 144, 789
TasI AATT 3 cut(s) 276, 363, 453
TatI WGTACW 1 cut(s) 300
Tru1I TTAA 3 cut(s) 153, 366, 573
Tru9I TTAA 3 cut(s) 153, 366, 573
TscAI CASTG 2 cut(s) 338, 426
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 8 cut(s) 5, 221, 460, 629, 650, 653, 656, 659
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 72
TspRI CASTG 2 cut(s) 338, 426
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 608
VspI ATTAAT 1 cut(s) 366
XapI RAATTY 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.