Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004744

Physical Location & Seq

Genomic Coordinates Reverse (-)

23352952 .. 23357670

Gene Structure 4719 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1119800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3636 bp

ATGTACCTCTACAGCCTCACTCTCCAGCGCGCCACCGGCATAGTATGCGCCATCAACGGCAATTTCTCCGGCGGCAAGGCCCAGGAGATCGTCGTCGCCCGCGGCAAAGTTCTCGACCTTCTCCGTCCCGACGAAAATGGTAAGATCCAGACTCTCCTCTCTGTTGAAATTTTCGGCGCAATTAGGTCGTTAGCTCAGTTTAGGCTCACTGGCTCTCAGAAGGACTATATTGTTGTCGGGTCCGATTCGGGTCGGATTGTGATTCTAGAGTATAATAAGGAGAAGAATGTGTTTGATAAGATTCACCAGGAGACTTTCGGGAAATCGGGTTGTCGAAGGATAGTTCCGGGTCAGTATTTGGCCATTGACCCCAAGGGGAGGGCTGTTATGGTTGGGGCATGTGAGAAGCAGAAGCTTGTTTACGTTTTGAATAGGGATACTTCTGCTAGGTTGACGATTTCGTCTCCGTTGGAGGCTCACAAGTCGCATACGATTGTGTATTCAATTTGTGGGGTTGATTGTGGGTTTGATAATCCCATGTTTGCTGCCATTGAGTTGGATTATGCGGAAGCTGATCAGGACTCTACTGGGCAGGCGGCGAATGAGGCGCAGAAGCATTTGACTTTCTATGAGCTCGATCTTGGCCTCAATCACGTATCAAGGAAGTGGTCAGATCAGGTTGATAATGGTGCAAATATGCTGGTCACTGTTCCTGGAGGTGGGGATGGGCCAAGCGGTGTATTGGTCTGTGCGGAAAATTTTGTGATTTATAAGAACCAGGATAAACCGGATCTCAGGGCTGTGATTCCTAGGCGTGCAGATTTGCCTGCAGAGCGTGGGGTTCTTATAGTTTCAGCGGCTATGCACAAGCAGAAATCAATGTTCTTTTTTCTTTTGCAGACAGAGTATGGAGATATATTTAAGGTTACTCTGGATCATGATAACGACAAGGTCTCAGAATTAAAGATCAAGTATTTTGATACAATTCCGGTTACAACTTCAATGTGTGTGTTAAAATCTGGGTTTTTGTTTGCTGCGTCAGAGTTTGGGAATCACTCTTTGTACCAGTTCCAGGCAATAGGGGAAGAACCTGACGTGGAGTCTTCATCAGCCACATTGATGGAAACGGAAGAAGGTTTCCAGCCTTTGTTTTTCCAGCCCAGAAAACTAAAAAACCTTGTTAGGATTGATCAAGTTGAGAGCTTGATGCCGATAATGGATATGAAGGTCAATAATCTCTTTGAGGAAGAAACACCTCAAATATTTACACTTTGTGGGCGGGGTCCTCGTTCATCCCTCAGGATACTAAGACCTGGTTTAGCTATCAGTGAGATGGCCGTGTCAGAGCTTCCTGGTGTACCGAGTGCGGTCTGGACAGTGAAGAAGAATGTGTCCGATGAATTTGATGCATACATTGTGGTGTCATTTGCAAATGCTACTCTTGTGCTTTCAATTGGTGAGACAGTTGAAGAAGTTAGTGACAGTGGGTTTCTTGACACTACCCCATCACTTGCAGTTTCTTTAATAGGTGATGATTCTCTCATGCAAGTGCACCCAAATGGAATTAGGCATATTAGGGAAGATGGACGTATTAATGAATGGAGAACTCCTGGAAAGAGGACGATTGTAAAGGTTGGCTCTAATAGACTTCAGGTAGTTATTGCACTGAGCGGAGGAGAACTCATATATTTTGAGGTTGATATGACGGGTCAGTTAATGGAGGTGGAGAAACATGAAATGTCTGGAGATGTAGCCTGTCTAGACATTGCCCCTGTACCTGAAGGGAGACAGAGATCTCGTTTCCTTGCCGTTGGTTCATATGACAGCACCATTCGTATTTTGTCATTGGATCCTGATGACTGTATGCAAATTCTTAGTGTGCAAAGTCTTTCTGGAGTTCCAGAATCTCTCCTCTTTCTTGAGGTTCAAGCATCAATTGGTGGGGAGGATGGTGCTGATCATCCTGCTAGCCTTTTCCTTAATGCTGGTTTACGCTCTGGGATTTTGTTCAGAACAGTGGTGGATATGGTCACAGGTCAACTCTCTGATTCCCGTTCTCGATTCTTGGGACTAAGAGCACCAAAGCTATTTTCTATTAGTGTGAGAGGCAAGCATGCTATGCTTTGCTTGTCAAGTCGGCCCTGGCTTGGTTATATCCATCAAGGACATTTTCTCTTAACACCCCTATCGTATGAGACCCTTGAATATGCTGCCTCATTTTCATCCGATCAATGTTCGGAAGGTGTAGTTTCTGTTGCTGGAAATGCTTTGAGGGTTTTTACTATTGAAAGATTGGGAGAAACATTTAATGAAACTGTGATTCCACTGAGGTACACCCCTAGAAAGTTTATCATTCAGCTCAAACGAAAGCTTTTGGTAATTATTGAAAGTGATCAAGGAGCGTTCACAGCAGAAGAGCGTGAAGCTGCCAAAAAAGAGTGTTTTGAGGCTGCAGGTTTAGGTGACAATGGAAACGGTAATGTAGATCAGATGGAAAATGGCGGGGATGAGGAGGATCCCTTGGCTGATGAGCACTACGGGTATCCTAAGGCAGAGTCTGACAAATGGGTGTCTTGCATTAGAGTTCTTGACCCCAAGACAGCAACTACAACATGTCTGCTGGAGCTTCAGGATAATGAAGCTGCATTCAGTATCTGTACAGTGAATTTCCATGACAAGGAGTATGGTACGCTTTTAGCTGTTGGCACAGCGAAGGGACTGCAGTTTTGGCCAAAAAAGAGTGTAACAGCAGGCTATATACATATATATAGATTCCTAGATGATGGAAAGTCCCTTGAACTTTTGCACAAGACACAAGTGGATGGTGTTCCTCTTGCCTTGTGCCAGTTTCAAGGAAGGTTACTAGCTGGGATCGGACCAGTGCTCAGACTCTATGATTTGGGGAAGAAAAGATTACTCAGGAAGTGTGAGAATAAGCTGTTTCCCAACACAATTGCGTCAATCCAAACTTATCGTGATCGGATTTATGTAGGTGACATTCAAGAGTCATTCCACTACTGCAAGTATAGGCGGGATGAGAATCAATTGTATATCTTTGCTGATGATTGTGTTCCAAGATGGCTTACAGCATCCTTCCATATAGATTTTGACACCATGGCAGGTGCAGACAAGTTTGGGAATGTTTATTTTGTGCGGTTACCACAGGATGTTTCAGATGAGATAGAAGAAGATCCAACGGGTGGGAGGATAAAATGGGAGCAGGGAAAGTTGAATGGCGCTCCCAACAAAGTAGAGGAGATAGTGCAGTATCATGTTGGTGATGTTGTCAGCTGCTTGCAGAAGGCATCTCTAATTCCAGGTGGTGGGGAGTGCATCATCTATGGAACTGTGATGGGTAGCTTGGGTTCCTTACTTGCGTTCACCTCACGTGATGATGTTGACTTCTTTTCTCACTTGGAGATGTATATGAGGCAGGAGCATCCACCCTTGTGTGGTAGAGATCACATGGCTTATAGATCAGCGTATTTCCCGGTCAAGGATGTGATTGATGGAGATCTGTGTGAGCAGTTCCCAACATTGCCCATGGATCTGCAGAGAAAAATTGCAGATGAGTTGGATAGAACTCCTGGAGAGATACTGAAGAAGCTTGAGGAAATTCGAAACAAGATCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005689 GO:0005730 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009555 GO:0009791 GO:0009846 GO:0009856 GO:0009892 GO:0009894 GO:0009895 GO:0009908 GO:0009987 GO:0010467 GO:0010605 GO:0016070 GO:0016071 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035670 GO:0042176 GO:0042177 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0046483 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051704 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071005 GO:0071011 GO:0071013 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0099402 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

CPSF_A MMS1_N

Protein Analysis

1212

Amino Acids

134.55

Weight (kDa)

5.25

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_RSE1_1st	PF10433	14 - 370	1e-122	RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_2nd	PF23726	442 - 762	3.2e-86	RSE1/DDB1/CPSF1 second beta-propeller
CPSF_A	PF03178	858 - 1177	5.9e-95	CPSF A subunit region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55200 AT3G55200 AT3G55200 AT3G55220
fragaria_vesca	FvH4_1g28630
malus_domestica	MD01G1119800.v1.1 MD07G1189000.v1.1
prunus_persica	Prupe.2G226500_v2.0.a1 Prupe.2G226500_v2.0.a1
pyrus_communis	pycom07g17890
rosa_chinensis	RchiOBHm_Chr1g0365341 RchiOBHm_Chr3g0492631
rosa_laevigata	RLG00000022753 RLG00000027428
rosa_multiflora	Rmu_sc0000831.1_g000021 Rmu_sc0008527.1_g000001 Rmu_sc0010918.1_g000001
rosa_roxburghii	Rroxscaffold_4G00291290 Rroxscaffold_6G00392170
rosa_rugosa	Rorug01G0321300 Rorug03G0255200
rosa_samantha	Rh1AG330200 Rh1BG291700 Rh1CG308300 Rh1DG323400 Rh3AG305400 Rh3BG341900 Rh3CG339300 Rh3DG341200
rosa_wichuraiana	Rw1G029280 Rw3G026890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	771
AarI	CACCTGC	1 cut(s)	3110
AasI	GACNNNNNNGTC	1 cut(s)	460
Acc36I	ACCTGC	2 cut(s)	2444, 3110
AccB7I	CCANNNNNTGG	2 cut(s)	3200, 3323
AccBSI	CCGCTC	1 cut(s)	1671
AccII	CGCG	2 cut(s)	30, 102
AcoI	YGGCCR	3 cut(s)	360, 1335, 2729
AcsI	RAATTY	6 cut(s)	168, 757, 1400, 1869, 2665, 3615
AcuI	CTGAAG	4 cut(s)	1634, 1800, 2612, 3620
AcvI	CACGTG	1 cut(s)	3389
AdeI	CACNNNGTG	1 cut(s)	1274
AfaI	GTAC	7 cut(s)	5, 1064, 1359, 1776, 2333, 2659, 2689
AfiI	CCNNNNNNNGG	9 cut(s)	378, 719, 1072, 2147, 2677, 3200, 3323, 3452, 3496
AflIII	ACRYGT	1 cut(s)	2612
AhdI	GACNNNNNGTC	1 cut(s)	1099
AjiI	CACGTC	1 cut(s)	1096
AjuI	GAANNNNNNNTTGG	2 cut(s)	2074, 2106
AleI	CACNNNNGTG	1 cut(s)	3448
Alw21I	GWGCWC	5 cut(s)	636, 1554, 2080, 2535, 2886
Alw26I	GTCTC	6 cut(s)	305, 468, 958, 1454, 1780, 2189
Alw44I	GTGCAC	1 cut(s)	1550
AlwNI	CAGNNNCTG	2 cut(s)	2558, 2655
AoxI	GGCC	7 cut(s)	78, 360, 643, 728, 1335, 2138, 2729
ApaLI	GTGCAC	1 cut(s)	1550
ApeKI	GCWGC	7 cut(s)	545, 1034, 2210, 2426, 2450, 2642, 3291
ApoI	RAATTY	6 cut(s)	168, 757, 1400, 1869, 2665, 3615
ArsI	GACNNNNNNTTYG	2 cut(s)	2709, 2741
AseI	ATTAAT	1 cut(s)	1593
AspA2I	CCTAGG	1 cut(s)	809
AspLEI	GCGC	6 cut(s)	30, 32, 50, 179, 610, 3239
AspS9I	GGNCC	6 cut(s)	79, 240, 728, 1283, 2139, 2876
AsuC2I	CCSGG	2 cut(s)	348, 3491
AsuHPI	GGTGA	7 cut(s)	296, 1469, 1541, 2474, 3005, 3290, 3373
AsuII	TTCGAA	1 cut(s)	3619
AsuNHI	GCTAGC	1 cut(s)	1967
AvaII	GGWCC	3 cut(s)	240, 1283, 2876
AvrII	CCTAGG	1 cut(s)	809
AxyI	CCTNAGG	2 cut(s)	1298, 2547
BaeGI	GKGCMC	1 cut(s)	1554
BalI	TGGCCA	2 cut(s)	362, 2731
BamHI	GGATCC	2 cut(s)	1849, 2515
BanII	GRGCYC	1 cut(s)	636
BarI	GAAGNNNNNNTAC	2 cut(s)	404, 436
BbrPI	CACGTG	1 cut(s)	3389
BbsI	GAAGAC	1 cut(s)	1095
Bbv12I	GWGCWC	5 cut(s)	636, 1554, 2080, 2535, 2886
BbvI	GCAGC	7 cut(s)	532, 1021, 2197, 2413, 2437, 2629, 3278
BceAI	ACGGC	3 cut(s)	73, 1322, 1793
BcgI	CGANNNNNNTGC	6 cut(s)	94, 128, 168, 202, 2701, 2735
BciVI	GTATCC	3 cut(s)	430, 1296, 2553
BclI	TGATCA	4 cut(s)	574, 1189, 1957, 2392
BcnI	CCSGG	2 cut(s)	348, 3491
BcoDI	GTCTC	6 cut(s)	305, 468, 958, 1454, 1780, 2189
BfaI	CTAG	8 cut(s)	266, 447, 810, 1760, 1968, 2340, 2777, 2864
BfmI	CTRYAG	5 cut(s)	10, 828, 2451, 2720, 3551
BfoI	RGCGCY	1 cut(s)	3240
BfuAI	ACCTGC	2 cut(s)	2444, 3110
BfuI	GTATCC	3 cut(s)	430, 1296, 2553
BglII	AGATCT	2 cut(s)	1793, 3514
BlnI	CCTAGG	1 cut(s)	809
Bme18I	GGWCC	3 cut(s)	240, 1283, 2876
BmeRI	GACNNNNNGTC	1 cut(s)	1099
BmgBI	CACGTC	1 cut(s)	1096
BmgT120I	GGNCC	6 cut(s)	79, 240, 728, 1283, 2139, 2876
BmiI	GGNNCC	5 cut(s)	241, 1284, 1851, 2517, 3367
BmrI	ACTGGG	1 cut(s)	597
BmsI	GCATC	7 cut(s)	1197, 1396, 1940, 3098, 3314, 3342, 3448
BmtI	GCTAGC	1 cut(s)	1971
BmuI	ACTGGG	1 cut(s)	597
BpiI	GAAGAC	1 cut(s)	1095
BplI	GAGNNNNNCTC	2 cut(s)	1665, 1697
BpmI	CTGGAG	6 cut(s)	8, 735, 1764, 1914, 2642, 3609
Bpu14I	TTCGAA	1 cut(s)	3619
BpuEI	CTTGAG	2 cut(s)	1940, 3629
BpuMI	CCSGG	2 cut(s)	348, 3491
BsaAI	YACGTR	2 cut(s)	655, 3389
BsaBI	GATNNNNATC	2 cut(s)	3039, 3513
BsaI	GGTCTC	2 cut(s)	958, 2189
BsaJI	CCNNGG	8 cut(s)	81, 100, 372, 809, 2141, 2520, 3114, 3543
BsaWI	WCCGGW	2 cut(s)	787, 988
Bsc4I	CCNNNNNNNGG	9 cut(s)	378, 719, 1072, 2147, 2677, 3200, 3323, 3452, 3496
Bse118I	RCCGGY	1 cut(s)	35
Bse1I	ACTGG	5 cut(s)	214, 592, 1066, 2845, 2879
Bse21I	CCTNAGG	2 cut(s)	1298, 2547
Bse3DI	GCAATG	2 cut(s)	1764, 3536
Bse8I	GATNNNNATC	2 cut(s)	3039, 3513
BseDI	CCNNGG	8 cut(s)	81, 100, 372, 809, 2141, 2520, 3114, 3543
BseJI	GATNNNNATC	2 cut(s)	3039, 3513
BseLI	CCNNNNNNNGG	9 cut(s)	378, 719, 1072, 2147, 2677, 3200, 3323, 3452, 3496
BseMI	GCAATG	2 cut(s)	1764, 3536
BseMII	CTCAG	9 cut(s)	209, 230, 808, 969, 1312, 1658, 2318, 2899, 2932
BseNI	ACTGG	5 cut(s)	214, 592, 1066, 2845, 2879
BsePI	GCGCGC	1 cut(s)	28
BseRI	GAGGAG	5 cut(s)	146, 1689, 1901, 2525, 3269
BseSI	GKGCMC	1 cut(s)	1554
BseXI	GCAGC	7 cut(s)	532, 1021, 2197, 2413, 2437, 2629, 3278
BseYI	CCCAGC	1 cut(s)	2867
BsgI	GTGCAG	3 cut(s)	837, 3144, 3284
Bsh1236I	CGCG	2 cut(s)	30, 102
BshFI	GGCC	7 cut(s)	80, 362, 645, 730, 1337, 2140, 2731
BsiHKAI	GWGCWC	5 cut(s)	636, 1554, 2080, 2535, 2886
BsiSI	CCGG	6 cut(s)	36, 69, 347, 788, 989, 3491
BslFI	GGGAC	4 cut(s)	111, 2082, 2730, 2776
BslI	CCNNNNNNNGG	9 cut(s)	378, 719, 1072, 2147, 2677, 3200, 3323, 3452, 3496
BsmAI	GTCTC	6 cut(s)	305, 468, 958, 1454, 1780, 2189
BsmBI	CGTCTC	1 cut(s)	468
BsmFI	GGGAC	4 cut(s)	111, 2082, 2730, 2776
BsmI	GAATGC	1 cut(s)	2645
BsnI	GGCC	7 cut(s)	80, 362, 645, 730, 1337, 2140, 2731
Bso31I	GGTCTC	2 cut(s)	958, 2189
Bsp119I	TTCGAA	1 cut(s)	3619
Bsp1286I	GDGCHC	5 cut(s)	636, 1554, 2080, 2535, 2886
Bsp1407I	TGTACA	1 cut(s)	2657
Bsp19I	CCATGG	2 cut(s)	3114, 3543
BspANI	GGCC	7 cut(s)	80, 362, 645, 730, 1337, 2140, 2731
BspCNI	CTCAG	9 cut(s)	208, 229, 807, 968, 1311, 1659, 2319, 2898, 2931
BspFNI	CGCG	2 cut(s)	30, 102
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	5 cut(s)	241, 1284, 1851, 2517, 3367
BspMAI	CTGCAG	4 cut(s)	832, 2455, 2724, 3555
BspMI	ACCTGC	2 cut(s)	2444, 3110
BspOI	GCTAGC	1 cut(s)	1971
BspQI	GCTCTTC	1 cut(s)	2409
BspT104I	TTCGAA	1 cut(s)	3619
BspTNI	GGTCTC	2 cut(s)	958, 2189
BsrBI	CCGCTC	1 cut(s)	1671
BsrDI	GCAATG	2 cut(s)	1764, 3536
BsrFI	RCCGGY	1 cut(s)	35
BsrGI	TGTACA	1 cut(s)	2657
BsrI	ACTGG	5 cut(s)	214, 592, 1066, 2845, 2879
BssAI	RCCGGY	1 cut(s)	35
BssECI	CCNNGG	8 cut(s)	81, 100, 372, 809, 2141, 2520, 3114, 3543
BssHII	GCGCGC	1 cut(s)	28
BssT1I	CCWWGG	5 cut(s)	372, 809, 2520, 3114, 3543
Bst6I	CTCTTC	1 cut(s)	2409
BstAPI	GCANNNNNTGC	2 cut(s)	45, 2119
BstAUI	TGTACA	1 cut(s)	2657
BstBAI	YACGTR	2 cut(s)	655, 3389
BstBI	TTCGAA	1 cut(s)	3619
BstDSI	CCRYGG	3 cut(s)	100, 3114, 3543
BstEII	GGTNACC	1 cut(s)	3156
BstFNI	CGCG	2 cut(s)	30, 102
BstH2I	RGCGCY	1 cut(s)	3240
BstHHI	GCGC	6 cut(s)	30, 32, 50, 179, 610, 3239
BstMAI	GTCTC	6 cut(s)	305, 468, 958, 1454, 1780, 2189
BstMWI	GCNNNNNNNGC	8 cut(s)	36, 45, 605, 832, 2119, 2264, 2408, 2728
BstNSI	RCATGY	3 cut(s)	402, 2117, 2616
BstPI	GGTNACC	1 cut(s)	3156
BstSFI	CTRYAG	5 cut(s)	10, 828, 2451, 2720, 3551
BstSLI	GKGCMC	1 cut(s)	1554
BstUI	CGCG	2 cut(s)	30, 102
BstV1I	GCAGC	7 cut(s)	532, 1021, 2197, 2413, 2437, 2629, 3278
BstV2I	GAAGAC	1 cut(s)	1095
BstX2I	RGATCY	8 cut(s)	144, 790, 1793, 1849, 2515, 3190, 3514, 3547
BstXI	CCANNNNNNTGG	2 cut(s)	556, 1120
BstYI	RGATCY	8 cut(s)	144, 790, 1793, 1849, 2515, 3190, 3514, 3547
Bsu36I	CCTNAGG	2 cut(s)	1298, 2547
BsuI	GTATCC	3 cut(s)	430, 1296, 2553
BsuRI	GGCC	7 cut(s)	80, 362, 645, 730, 1337, 2140, 2731
BtgI	CCRYGG	3 cut(s)	100, 3114, 3543
BtrI	CACGTC	1 cut(s)	1096
BveI	ACCTGC	2 cut(s)	2444, 3110
CaiI	CAGNNNCTG	2 cut(s)	2558, 2655
CciI	TCATGA	1 cut(s)	937
CfoI	GCGC	6 cut(s)	30, 32, 50, 179, 610, 3239
Cfr10I	RCCGGY	1 cut(s)	35
Cfr13I	GGNCC	6 cut(s)	79, 240, 728, 1283, 2139, 2876
Cfr42I	CCGCGG	1 cut(s)	103
CseI	GACGC	2 cut(s)	1026, 2946
CsiI	ACCWGGT	1 cut(s)	1312
Csp6I	GTAC	7 cut(s)	4, 1063, 1358, 1775, 2332, 2658, 2688
CviQI	GTAC	7 cut(s)	4, 1063, 1358, 1775, 2332, 2658, 2688
DraIII	CACNNNGTG	1 cut(s)	1274
DrdI	GACNNNNNNGTC	1 cut(s)	460
DriI	GACNNNNNGTC	1 cut(s)	1099
DseDI	GACNNNNNNGTC	1 cut(s)	460
EaeI	YGGCCR	3 cut(s)	360, 1335, 2729
Eam1104I	CTCTTC	1 cut(s)	2409
Eam1105I	GACNNNNNGTC	1 cut(s)	1099
EarI	CTCTTC	1 cut(s)	2409
Ecl136II	GAGCTC	1 cut(s)	634
Eco130I	CCWWGG	5 cut(s)	372, 809, 2520, 3114, 3543
Eco24I	GRGCYC	1 cut(s)	636
Eco31I	GGTCTC	2 cut(s)	958, 2189
Eco47I	GGWCC	3 cut(s)	240, 1283, 2876
Eco53kI	GAGCTC	1 cut(s)	634
Eco57I	CTGAAG	4 cut(s)	1634, 1800, 2612, 3620
Eco72I	CACGTG	1 cut(s)	3389
Eco81I	CCTNAGG	2 cut(s)	1298, 2547
Eco91I	GGTNACC	1 cut(s)	3156
EcoICRI	GAGCTC	1 cut(s)	634
EcoO109I	RGGNCCY	1 cut(s)	1283
EcoO65I	GGTNACC	1 cut(s)	3156
EcoT14I	CCWWGG	5 cut(s)	372, 809, 2520, 3114, 3543
EcoT22I	ATGCAT	1 cut(s)	1411
EcoT38I	GRGCYC	1 cut(s)	636
ErhI	CCWWGG	5 cut(s)	372, 809, 2520, 3114, 3543
Esp3I	CGTCTC	1 cut(s)	468
FaqI	GGGAC	4 cut(s)	111, 2082, 2730, 2776
FauI	CCCGC	4 cut(s)	107, 1272, 2495, 3024
FauNDI	CATATG	1 cut(s)	1819
FbaI	TGATCA	4 cut(s)	574, 1189, 1957, 2392
FriOI	GRGCYC	1 cut(s)	636
FspBI	CTAG	8 cut(s)	266, 447, 810, 1760, 1968, 2340, 2777, 2864
GlaI	GCGC	6 cut(s)	29, 31, 49, 178, 609, 3238
GsaI	CCCAGC	1 cut(s)	2871
GsuI	CTGGAG	6 cut(s)	8, 735, 1764, 1914, 2642, 3609
HaeII	RGCGCY	1 cut(s)	3240
HaeIII	GGCC	7 cut(s)	80, 362, 645, 730, 1337, 2140, 2731
HapII	CCGG	6 cut(s)	36, 69, 347, 788, 989, 3491
HgaI	GACGC	2 cut(s)	1026, 2946
HhaI	GCGC	6 cut(s)	30, 32, 50, 179, 610, 3239
Hin6I	GCGC	6 cut(s)	28, 30, 48, 177, 608, 3237
HinP1I	GCGC	6 cut(s)	28, 30, 48, 177, 608, 3237
HincII	GTYRAC	3 cut(s)	453, 2039, 3400
HindII	GTYRAC	3 cut(s)	453, 2039, 3400
HindIII	AAGCTT	3 cut(s)	413, 2369, 3605
HpaII	CCGG	6 cut(s)	36, 69, 347, 788, 989, 3491
HphI	GGTGA	7 cut(s)	296, 1469, 1541, 2474, 3005, 3290, 3373
Hpy99I	CGWCG	3 cut(s)	95, 98, 134
HpyCH4IV	ACGT	5 cut(s)	423, 654, 1095, 1588, 3388
HpyF10VI	GCNNNNNNNGC	8 cut(s)	36, 45, 605, 832, 2119, 2264, 2408, 2728
HpySE526I	ACGT	5 cut(s)	423, 654, 1095, 1588, 3388
HspAI	GCGC	6 cut(s)	28, 30, 48, 177, 608, 3237
Ksp22I	TGATCA	4 cut(s)	574, 1189, 1957, 2392
KspI	CCGCGG	1 cut(s)	103
LguI	GCTCTTC	1 cut(s)	2409
LmnI	GCTCC	5 cut(s)	2399, 2623, 3217, 3244, 3436
Lsp1109I	GCAGC	7 cut(s)	532, 1021, 2197, 2413, 2437, 2629, 3278
LweI	GCATC	7 cut(s)	1197, 1396, 1940, 3098, 3314, 3342, 3448
MabI	ACCWGGT	1 cut(s)	1312
MaeI	CTAG	8 cut(s)	266, 447, 810, 1760, 1968, 2340, 2777, 2864
MaeII	ACGT	5 cut(s)	423, 654, 1095, 1588, 3388
MbiI	CCGCTC	1 cut(s)	1671
MfeI	CAATTG	4 cut(s)	1452, 1935, 2952, 3044
MflI	RGATCY	8 cut(s)	144, 790, 1793, 1849, 2515, 3190, 3514, 3547
MhlI	GDGCHC	5 cut(s)	636, 1554, 2080, 2535, 2886
MlsI	TGGCCA	2 cut(s)	362, 2731
MluNI	TGGCCA	2 cut(s)	362, 2731
MlyI	GAGTC	6 cut(s)	145, 575, 1109, 2564, 2883, 3014
MmeI	TCCRAC	5 cut(s)	233, 450, 537, 3218, 3555
Mox20I	TGGCCA	2 cut(s)	362, 2731
Mph1103I	ATGCAT	1 cut(s)	1411
MscI	TGGCCA	2 cut(s)	362, 2731
MslI	CAYNNNNRTG	6 cut(s)	1118, 1418, 1547, 1557, 3276, 3448
Msp20I	TGGCCA	2 cut(s)	362, 2731
MspA1I	CMGCKG	3 cut(s)	102, 857, 3291
MspI	CCGG	6 cut(s)	36, 69, 347, 788, 989, 3491
MunI	CAATTG	4 cut(s)	1452, 1935, 2952, 3044
Mva1269I	GAATGC	1 cut(s)	2645
MvnI	CGCG	2 cut(s)	30, 102
MwoI	GCNNNNNNNGC	8 cut(s)	36, 45, 605, 832, 2119, 2264, 2408, 2728
NciI	CCSGG	2 cut(s)	348, 3491
NcoI	CCATGG	2 cut(s)	3114, 3543
NdeI	CATATG	1 cut(s)	1819
NheI	GCTAGC	1 cut(s)	1967
NlaIV	GGNNCC	5 cut(s)	241, 1284, 1851, 2517, 3367
NmuCI	GTSAC	5 cut(s)	703, 1478, 2029, 2462, 2993
NsiI	ATGCAT	1 cut(s)	1411
NspI	RCATGY	3 cut(s)	402, 2117, 2616
NspV	TTCGAA	1 cut(s)	3619
OliI	CACNNNNGTG	1 cut(s)	3448
PaeI	GCATGC	1 cut(s)	2117
PagI	TCATGA	1 cut(s)	937
PaqCI	CACCTGC	1 cut(s)	3110
PauI	GCGCGC	1 cut(s)	28
PciI	ACATGT	1 cut(s)	2612
PciSI	GCTCTTC	1 cut(s)	2409
PctI	GAATGC	1 cut(s)	2645
PflFI	GACNNNGTC	1 cut(s)	950
PflMI	CCANNNNNTGG	2 cut(s)	3200, 3323
PfoI	TCCNGGA	3 cut(s)	712, 1609, 3586
PleI	GAGTC	6 cut(s)	145, 575, 1108, 2563, 2883, 3013
PmaCI	CACGTG	1 cut(s)	3389
PmlI	CACGTG	1 cut(s)	3389
PpsI	GAGTC	6 cut(s)	145, 575, 1108, 2563, 2883, 3013
Ppu21I	YACGTR	2 cut(s)	655, 3389
PpuMI	RGGWCCY	1 cut(s)	1283
PscI	ACATGT	1 cut(s)	2612
PshBI	ATTAAT	1 cut(s)	1593
PsiI	TTATAA	1 cut(s)	771
Psp124BI	GAGCTC	1 cut(s)	636
Psp5II	RGGWCCY	1 cut(s)	1283
PspCI	CACGTG	1 cut(s)	3389
PspEI	GGTNACC	1 cut(s)	3156
PspFI	CCCAGC	1 cut(s)	2867
PspN4I	GGNNCC	5 cut(s)	241, 1284, 1851, 2517, 3367
PspPI	GGNCC	6 cut(s)	79, 240, 728, 1283, 2139, 2876
PspPPI	RGGWCCY	1 cut(s)	1283
PsrI	GAACNNNNNNTAC	2 cut(s)	3349, 3381
PstI	CTGCAG	4 cut(s)	832, 2455, 2724, 3555
PstNI	CAGNNNCTG	2 cut(s)	2558, 2655
PsuI	RGATCY	8 cut(s)	144, 790, 1793, 1849, 2515, 3190, 3514, 3547
PsyI	GACNNNGTC	1 cut(s)	950
PteI	GCGCGC	1 cut(s)	28
PvuII	CAGCTG	1 cut(s)	3291
RsaI	GTAC	7 cut(s)	5, 1064, 1359, 1776, 2333, 2659, 2689
RsaNI	GTAC	7 cut(s)	4, 1063, 1358, 1775, 2332, 2658, 2688
RseI	CAYNNNNRTG	6 cut(s)	1118, 1418, 1547, 1557, 3276, 3448
SacI	GAGCTC	1 cut(s)	636
SacII	CCGCGG	1 cut(s)	103
SapI	GCTCTTC	1 cut(s)	2409
Sau96I	GGNCC	6 cut(s)	79, 240, 728, 1283, 2139, 2876
SchI	GAGTC	6 cut(s)	145, 575, 1109, 2564, 2883, 3014
SduI	GDGCHC	5 cut(s)	636, 1554, 2080, 2535, 2886
SexAI	ACCWGGT	1 cut(s)	1312
SfaNI	GCATC	7 cut(s)	1197, 1396, 1940, 3098, 3314, 3342, 3448
SfcI	CTRYAG	5 cut(s)	10, 828, 2451, 2720, 3551
Sfr303I	CCGCGG	1 cut(s)	103
SfuI	TTCGAA	1 cut(s)	3619
SgrBI	CCGCGG	1 cut(s)	103
SinI	GGWCC	3 cut(s)	240, 1283, 2876
SmiMI	CAYNNNNRTG	6 cut(s)	1118, 1418, 1547, 1557, 3276, 3448
SmlI	CTYRAG	2 cut(s)	1919, 3608
SmoI	CTYRAG	2 cut(s)	1919, 3608
SphI	GCATGC	1 cut(s)	2117
SspI	AATATT	1 cut(s)	1263
SspMI	CTAG	8 cut(s)	266, 447, 810, 1760, 1968, 2340, 2777, 2864
SstI	GAGCTC	1 cut(s)	636
StyI	CCWWGG	5 cut(s)	372, 809, 2520, 3114, 3543
TaiI	ACGT	5 cut(s)	426, 657, 1098, 1591, 3391
TaqI	TCGA	5 cut(s)	114, 334, 636, 2059, 3619
TatI	WGTACW	1 cut(s)	2657
TauI	GCSGC	4 cut(s)	75, 105, 599, 860
TseFI	GTSAC	5 cut(s)	703, 1478, 2029, 2462, 2993
TseI	GCWGC	7 cut(s)	545, 1034, 2210, 2426, 2450, 2642, 3291
Tsp45I	GTSAC	5 cut(s)	703, 1478, 2029, 2462, 2993
TspGWI	ACGGA	3 cut(s)	113, 456, 1142
Tth111I	GACNNNGTC	1 cut(s)	950
Van91I	CCANNNNNTGG	2 cut(s)	3200, 3323
VneI	GTGCAC	1 cut(s)	1550
VpaK11BI	GGWCC	3 cut(s)	240, 1283, 2876
VspI	ATTAAT	1 cut(s)	1593
XapI	RAATTY	6 cut(s)	168, 757, 1400, 1869, 2665, 3615
XbaI	TCTAGA	2 cut(s)	265, 1759
XceI	RCATGY	3 cut(s)	402, 2117, 2616
XmaJI	CCTAGG	1 cut(s)	809
XspI	CTAG	8 cut(s)	266, 447, 810, 1760, 1968, 2340, 2777, 2864
Zsp2I	ATGCAT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.