Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004744

Physical Location & Seq

Genomic Coordinates Reverse (-)

26952890 .. 26955695

Gene Structure 2806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1189000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGTACCTCTACAGCCTCACTCTCCAGCGCGCCACCGGCATAGTCTGCGCCATCAACGGAAACTTCTCCGGCGGCAAGGCCCAGGAGATCGTAGTCGCCCGCGGCAAAGTTCTCGACCTTCTCCGCCCAGACGAAAATGGTAAGATCCAGACTCTCCTCTCTGTTGAAATTTTCGGCGCAATTAGGTCGTTAGCTCAGTTTAGGCTCACTGGGTCTCAGAAGGACTATATTGTTGTCGGGTCCGATTCGGGTCGGATTGTGATTCTTGAGTATAATAAGGAGAAGAATGTGTTTGATAAGATTCACCAGGAGACTTTCGGGAAATCGGGTTGTCGGAGGATAGTTCCGGGTCAGTATTTGGCTATTGATCCTAAGGGTAGGGCTGTTATGGTTGGGGCATGTGAGAAGCAGAAGCTTGTTTACGTTTTGAATAGGGATACTTCTGCTAGGTTGACAATTTCGTCGCCCTTGGAGGCGCACAAGTCACATACTATTGTGTATTCGATTTGTGGGGTTGACTGCGGGTTTGATAATCCCATGTTTGCTGCCATTGAGTTGGATTATGCGGAAGCTGATCAGGACTCTACTGGGCAGGCGGCGAACGAGGCGCAGAAGCATTTGACTTTCTATGAGCTTGATCTTGGGCTCAATCACGTATCGAGGAAGTGGTCGGATCAGGTTGATAATGGTGCAAATATGCTGGTCACGGTTCCTGGAGGTGGGGATGGTCCAAGCGGTGTATTGGTCTGTGCGGAAAATTTTGTGATTTATAAGAACCAGGATAAACCAGATCTCAGGGCTGTGATTCCTAGGCGTGCGGATTTGCCTGCAGAGCGTGGGGTTCTTATAGTTTCAGCAGCTATGCACAAGCAGAAGTCAATGTTCTTTTTTCTTTTACAGACAGAGTATGGAGATATATTTAAGGTTACTCTGGATCATGATAACGATAAGGTCTCAGAATTAAAAATCAAGTATTTTGATACAATTCCAGTTACAACTTCAATGTGTGTGTTAAAATCTGGGTTTTTGTTTGCTGCGTCAGAGTTTGGGAATCACTCTTTGTACCAGTTCCAGGCAATAGGAGAAGAGCCTGATGTGGAGTCTTCATCAGCTACATTGATGGAAACAGAAGAAGGTTTCCAGCCTTTGTTTTTCCAGCCCAGAAAACTTAAGAACCTTGTTAGGATTGATCAAGTTGAGAGCTTGATGCCGATAATGGATATGAAGGTCAATAATCTATTTGAGGAAGAAACACCTCAAATATTTACACTTTGTGGGCGGGGTCCTCGTTCATCCCTCAGGATACTAAGACCTGGTTTAGCTATCAGTGAGATGGCTGTGTCAGAGCTTCCTGGTGTACCGAGTGCGGTCTGGACTGTGAAGAAGAATGTGTCCGATGAATTTGATGCATACATCGTGGTGTCATTTGCAAATGCTACTCTTGTGCTTTCAATTGGTGAGACGGTTGAAGAAGTTAGTGACAGTGGGTTTCTAGACACTACCCCATCACTTGCAGTTTCTTTGATAGGTGATGATTCTCTGATGCAAGTGCACCCAAATGGAATTAGGCATATTAGGGAAGATGGACGTATTAATGAATGGAGAACTCCTGGAAAGAGGACGATTGTAAAGGTTGGCTCTAATAGACTTCAAGTAGTTATTGCACTGAGCGGAGGAGAACTTATATATTTTGAGGTTGATATGACTGGTCAGTTAATGGAGGTGGAGAAGCATGAAATGTCTGGAGATGTAGCTTGTCTAGACATTTCCCCGGTACCTGAAGGGAGACAGAGATCTCGTTTCCTTGCAGTTGGTTCATATGACAGCGTCATTCGTATTTTGTCATTGGATCCTGATGACTGTATGCAAATTCTTAGTGTGCAAAGTCTTTCTGGAGTTCCAGAATCTCTCCTCTTTCTTGAGGTTCAGGCCTCAATTGGTGGGGAGGATGGTGCTGATCATCCTGCTAACCTTTTCCTTAATGCTGGCTTACGCTCCGGGATTTTATTCAGAACTGTGGTGGATATGGTCACAGGTCAGCTCTCTGATTCCCGTTCTCGATTCTTGGGACTAAGAGCACCAAAGCTATTTTCAATTAGTGTGAGAGGCAAGCATGCTATGCTTTGCTTGTCAAGTCGGCCCTGGCTTGGTTATATCCATCAAGGACATTTTCTCTTAACACCACTATCGTATGAGACCCTTGAATATGCTGCCTCATTTTCATCCGATCAATGTTCGGAAGGTGTAGTTTCTGTTGCTGGAAATGCTTTGAGGGTATTTACTATTGAAAGATTGGGAGAAACATTTAATGAAACTGTGATTCCACTGAGGTATACCCCTAGAAAGTTTATCGTTCAACTCAAACGAAAGCTTTTGGTAATTATTGAAAGTGATCAAGGAGCATTCACAGCAGAAGAGCGTGAAGCTGCAAAAAAGGAGTGTTTTGAGGCTGCGGGTTTAGGTGAAAATGGAAACGGTAATGTAGAGCAGATGGAAAATGGTGGCGATGAGGAGGATCCCTTATCTGATGAGCACTATGGGTATCCTAAGGCAGAGTCTGACAAATGGGTGTCTTGCATTAGAGTTCTTGACCCCAAGACAGCAACTACAACATGTCTGCTGGAGCTTCAGGATAGCGAAGCTGCATTTAGTATTTGTACAGTGAATTTCCATGACAAGGAGTATGGTACACTTTTAGCTGTTGGCACAGCGAAGGGACTGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005689 GO:0005730 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009555 GO:0009791 GO:0009846 GO:0009856 GO:0009892 GO:0009894 GO:0009895 GO:0009908 GO:0009987 GO:0010467 GO:0010605 GO:0016070 GO:0016071 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035670 GO:0042176 GO:0042177 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0046483 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051704 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071005 GO:0071011 GO:0071013 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0099402 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

CPSF_A MMS1_N

Protein Analysis

909

Amino Acids

99.92

Weight (kDa)

5.11

Isoelectric Point (pI)

35.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_RSE1_1st	PF10433	14 - 370	5.5e-123	RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_2nd	PF23726	442 - 762	9.4e-86	RSE1/DDB1/CPSF1 second beta-propeller
CPSF_A	PF03178	858 - 905	1.4e-06	CPSF A subunit region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55200 AT3G55200 AT3G55200 AT3G55220
fragaria_vesca	FvH4_1g28630
malus_domestica	MD01G1119800.v1.1 MD07G1189000.v1.1
prunus_persica	Prupe.2G226500_v2.0.a1 Prupe.2G226500_v2.0.a1
pyrus_communis	pycom07g17890
rosa_chinensis	RchiOBHm_Chr1g0365341 RchiOBHm_Chr3g0492631
rosa_laevigata	RLG00000022753 RLG00000027428
rosa_multiflora	Rmu_sc0000831.1_g000021 Rmu_sc0008527.1_g000001 Rmu_sc0010918.1_g000001
rosa_roxburghii	Rroxscaffold_4G00291290 Rroxscaffold_6G00392170
rosa_rugosa	Rorug01G0321300 Rorug03G0255200
rosa_samantha	Rh1AG330200 Rh1BG291700 Rh1CG308300 Rh1DG323400 Rh3AG305400 Rh3BG341900 Rh3CG339300 Rh3DG341200
rosa_wichuraiana	Rw1G029280 Rw3G026890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	771
AasI	GACNNNNNNGTC	1 cut(s)	460
Acc65I	GGTACC	1 cut(s)	1774
AccB1I	GGYRCC	1 cut(s)	1774
AccBSI	CCGCTC	1 cut(s)	1671
AccI	GTMKAC	1 cut(s)	2333
AccII	CGCG	2 cut(s)	30, 102
AclWI	GGATC	8 cut(s)	139, 362, 681, 942, 1844, 1857, 2510, 2523
AcsI	RAATTY	5 cut(s)	168, 757, 1400, 1869, 2665
AcuI	CTGAAG	2 cut(s)	1800, 2612
AdeI	CACNNNGTG	1 cut(s)	1274
AfaI	GTAC	6 cut(s)	5, 1064, 1359, 1776, 2659, 2689
AfiI	CCNNNNNNNGG	4 cut(s)	719, 1072, 2147, 2677
AflII	CTTAAG	1 cut(s)	1169
AflIII	ACRYGT	1 cut(s)	2612
AjnI	CCWGG	9 cut(s)	81, 306, 712, 777, 1071, 1312, 1351, 1609, 2141
AjuI	GAANNNNNNNTTGG	2 cut(s)	2074, 2106
Alw21I	GWGCWC	3 cut(s)	1554, 2080, 2535
Alw26I	GTCTC	6 cut(s)	219, 305, 958, 1454, 1780, 2189
Alw44I	GTGCAC	1 cut(s)	1550
AlwI	GGATC	8 cut(s)	139, 362, 681, 942, 1844, 1857, 2510, 2523
AlwNI	CAGNNNCTG	1 cut(s)	2558
AoxI	GGCC	3 cut(s)	78, 1929, 2138
ApaLI	GTGCAC	1 cut(s)	1550
ApeKI	GCWGC	7 cut(s)	545, 857, 1034, 2210, 2426, 2450, 2642
ApoI	RAATTY	5 cut(s)	168, 757, 1400, 1869, 2665
ArsI	GACNNNNNNTTYG	2 cut(s)	509, 541
AseI	ATTAAT	1 cut(s)	1593
Asp718I	GGTACC	1 cut(s)	1774
AspA2I	CCTAGG	1 cut(s)	809
AspLEI	GCGC	6 cut(s)	30, 32, 50, 179, 478, 610
AspS9I	GGNCC	5 cut(s)	79, 240, 728, 1283, 2139
AsuC2I	CCSGG	3 cut(s)	348, 1772, 1999
AsuHPI	GGTGA	4 cut(s)	296, 1469, 1541, 2474
AvaII	GGWCC	3 cut(s)	240, 728, 1283
AvrII	CCTAGG	1 cut(s)	809
AxyI	CCTNAGG	3 cut(s)	372, 1298, 2547
BaeGI	GKGCMC	1 cut(s)	1554
BamHI	GGATCC	2 cut(s)	1849, 2515
BanI	GGYRCC	1 cut(s)	1774
BanII	GRGCYC	1 cut(s)	648
BarI	GAAGNNNNNNTAC	2 cut(s)	404, 436
BbsI	GAAGAC	1 cut(s)	1095
Bbv12I	GWGCWC	3 cut(s)	1554, 2080, 2535
BbvI	GCAGC	7 cut(s)	532, 869, 1021, 2197, 2413, 2437, 2629
BccI	CCATC	9 cut(s)	59, 719, 1114, 1327, 1513, 1577, 1943, 2166, 2485
BcgI	CGANNNNNNTGC	5 cut(s)	94, 128, 168, 202, 2701
BciT130I	CCWGG	9 cut(s)	83, 308, 714, 779, 1073, 1314, 1353, 1611, 2143
BciVI	GTATCC	3 cut(s)	430, 1296, 2553
BclI	TGATCA	4 cut(s)	574, 1189, 1957, 2392
BcnI	CCSGG	3 cut(s)	348, 1772, 1999
BcoDI	GTCTC	6 cut(s)	219, 305, 958, 1454, 1780, 2189
BfaI	CTAG	5 cut(s)	447, 810, 1493, 1760, 2340
BfmI	CTRYAG	2 cut(s)	10, 828
BfrI	CTTAAG	1 cut(s)	1169
BfuI	GTATCC	3 cut(s)	430, 1296, 2553
BglII	AGATCT	2 cut(s)	790, 1793
BlnI	CCTAGG	1 cut(s)	809
Bme18I	GGWCC	3 cut(s)	240, 728, 1283
BmgT120I	GGNCC	5 cut(s)	79, 240, 728, 1283, 2139
BmiI	GGNNCC	6 cut(s)	241, 711, 1284, 1776, 1851, 2517
BmrI	ACTGGG	2 cut(s)	219, 597
BmsI	GCATC	3 cut(s)	1197, 1396, 1533
BmuI	ACTGGG	2 cut(s)	219, 597
BpiI	GAAGAC	1 cut(s)	1095
BpmI	CTGGAG	5 cut(s)	8, 735, 1764, 1914, 2642
BpuEI	CTTGAG	2 cut(s)	287, 1940
BpuMI	CCSGG	3 cut(s)	348, 1772, 1999
BsaAI	YACGTR	1 cut(s)	655
BsaI	GGTCTC	3 cut(s)	219, 958, 2189
BsaJI	CCNNGG	6 cut(s)	81, 100, 468, 809, 1770, 2141
Bsc4I	CCNNNNNNNGG	4 cut(s)	719, 1072, 2147, 2677
Bse118I	RCCGGY	1 cut(s)	35
Bse1I	ACTGG	5 cut(s)	214, 592, 989, 1066, 1711
Bse21I	CCTNAGG	3 cut(s)	372, 1298, 2547
BseBI	CCWGG	9 cut(s)	83, 308, 714, 779, 1073, 1314, 1353, 1611, 2143
BseDI	CCNNGG	6 cut(s)	81, 100, 468, 809, 1770, 2141
BseGI	GGATG	5 cut(s)	730, 1292, 1954, 1960, 2222
BseLI	CCNNNNNNNGG	4 cut(s)	719, 1072, 2147, 2677
BseMII	CTCAG	7 cut(s)	209, 230, 808, 969, 1312, 1658, 2318
BseNI	ACTGG	5 cut(s)	214, 592, 989, 1066, 1711
BsePI	GCGCGC	1 cut(s)	28
BseRI	GAGGAG	4 cut(s)	146, 1689, 1901, 2525
BseSI	GKGCMC	1 cut(s)	1554
BseXI	GCAGC	7 cut(s)	532, 869, 1021, 2197, 2413, 2437, 2629
Bsh1236I	CGCG	2 cut(s)	30, 102
BshFI	GGCC	3 cut(s)	80, 1931, 2140
BshNI	GGYRCC	1 cut(s)	1774
BsiHKAI	GWGCWC	3 cut(s)	1554, 2080, 2535
BsiSI	CCGG	5 cut(s)	36, 69, 347, 1772, 1998
BslFI	GGGAC	1 cut(s)	2082
BslI	CCNNNNNNNGG	4 cut(s)	719, 1072, 2147, 2677
BsmAI	GTCTC	6 cut(s)	219, 305, 958, 1454, 1780, 2189
BsmBI	CGTCTC	1 cut(s)	1454
BsmFI	GGGAC	1 cut(s)	2082
BsmI	GAATGC	1 cut(s)	2402
BsnI	GGCC	3 cut(s)	80, 1931, 2140
Bso31I	GGTCTC	3 cut(s)	219, 958, 2189
Bsp1286I	GDGCHC	4 cut(s)	648, 1554, 2080, 2535
Bsp1407I	TGTACA	1 cut(s)	2657
BspANI	GGCC	3 cut(s)	80, 1931, 2140
BspCNI	CTCAG	7 cut(s)	208, 229, 807, 968, 1311, 1659, 2319
BspFNI	CGCG	2 cut(s)	30, 102
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	6 cut(s)	241, 711, 1284, 1776, 1851, 2517
BspMAI	CTGCAG	1 cut(s)	832
BspPI	GGATC	8 cut(s)	139, 362, 681, 942, 1844, 1857, 2510, 2523
BspQI	GCTCTTC	2 cut(s)	1080, 2409
BspT107I	GGYRCC	1 cut(s)	1774
BspTI	CTTAAG	1 cut(s)	1169
BspTNI	GGTCTC	3 cut(s)	219, 958, 2189
BsrBI	CCGCTC	1 cut(s)	1671
BsrFI	RCCGGY	1 cut(s)	35
BsrGI	TGTACA	1 cut(s)	2657
BsrI	ACTGG	5 cut(s)	214, 592, 989, 1066, 1711
BssAI	RCCGGY	1 cut(s)	35
BssECI	CCNNGG	6 cut(s)	81, 100, 468, 809, 1770, 2141
BssHII	GCGCGC	1 cut(s)	28
BssNAI	GTATAC	1 cut(s)	2334
BssT1I	CCWWGG	2 cut(s)	468, 809
Bst1107I	GTATAC	1 cut(s)	2334
Bst2UI	CCWGG	9 cut(s)	83, 308, 714, 779, 1073, 1314, 1353, 1611, 2143
Bst4CI	ACNGT	9 cut(s)	709, 1378, 1465, 1484, 1862, 2017, 2317, 2477, 2662
Bst6I	CTCTTC	2 cut(s)	1080, 2409
BstAFI	CTTAAG	1 cut(s)	1169
BstAPI	GCANNNNNTGC	2 cut(s)	45, 2119
BstAUI	TGTACA	1 cut(s)	2657
BstBAI	YACGTR	1 cut(s)	655
BstC8I	GCNNGC	8 cut(s)	30, 100, 594, 816, 828, 1987, 2111, 2115
BstDSI	CCRYGG	1 cut(s)	100
BstF5I	GGATG	5 cut(s)	730, 1292, 1954, 1960, 2222
BstFNI	CGCG	2 cut(s)	30, 102
BstHHI	GCGC	6 cut(s)	30, 32, 50, 179, 478, 610
BstMAI	GTCTC	6 cut(s)	219, 305, 958, 1454, 1780, 2189
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 45, 605, 832, 2119, 2264, 2408
BstNI	CCWGG	9 cut(s)	83, 308, 714, 779, 1073, 1314, 1353, 1611, 2143
BstNSI	RCATGY	3 cut(s)	402, 2117, 2616
BstSFI	CTRYAG	2 cut(s)	10, 828
BstSLI	GKGCMC	1 cut(s)	1554
BstUI	CGCG	2 cut(s)	30, 102
BstV1I	GCAGC	7 cut(s)	532, 869, 1021, 2197, 2413, 2437, 2629
BstV2I	GAAGAC	1 cut(s)	1095
BstX2I	RGATCY	5 cut(s)	144, 790, 1793, 1849, 2515
BstXI	CCANNNNNNTGG	1 cut(s)	556
BstYI	RGATCY	5 cut(s)	144, 790, 1793, 1849, 2515
BstZ17I	GTATAC	1 cut(s)	2334
Bsu36I	CCTNAGG	3 cut(s)	372, 1298, 2547
BsuI	GTATCC	3 cut(s)	430, 1296, 2553
BsuRI	GGCC	3 cut(s)	80, 1931, 2140
BtgI	CCRYGG	1 cut(s)	100
BtgZI	GCGATG	1 cut(s)	2520
BtsCI	GGATG	5 cut(s)	730, 1292, 1954, 1960, 2222
BtsIMutI	CAGTG	6 cut(s)	207, 1333, 1489, 1664, 2324, 2667
Cac8I	GCNNGC	8 cut(s)	30, 100, 594, 816, 828, 1987, 2111, 2115
CaiI	CAGNNNCTG	1 cut(s)	2558
CciI	TCATGA	1 cut(s)	937
CfoI	GCGC	6 cut(s)	30, 32, 50, 179, 478, 610
Cfr10I	RCCGGY	1 cut(s)	35
Cfr13I	GGNCC	5 cut(s)	79, 240, 728, 1283, 2139
Cfr42I	CCGCGG	1 cut(s)	103
CseI	GACGC	2 cut(s)	1026, 1816
CsiI	ACCWGGT	1 cut(s)	1312
Csp6I	GTAC	6 cut(s)	4, 1063, 1358, 1775, 2658, 2688
CviAII	CATG	7 cut(s)	399, 538, 938, 1733, 2114, 2613, 2672
CviQI	GTAC	6 cut(s)	4, 1063, 1358, 1775, 2658, 2688
DraIII	CACNNNGTG	1 cut(s)	1274
DrdI	GACNNNNNNGTC	1 cut(s)	460
DseDI	GACNNNNNNGTC	1 cut(s)	460
Eam1104I	CTCTTC	2 cut(s)	1080, 2409
EarI	CTCTTC	2 cut(s)	1080, 2409
EciI	GGCGGA	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	468, 809
Eco147I	AGGCCT	1 cut(s)	1931
Eco24I	GRGCYC	1 cut(s)	648
Eco31I	GGTCTC	3 cut(s)	219, 958, 2189
Eco47I	GGWCC	3 cut(s)	240, 728, 1283
Eco57I	CTGAAG	2 cut(s)	1800, 2612
Eco81I	CCTNAGG	3 cut(s)	372, 1298, 2547
EcoO109I	RGGNCCY	1 cut(s)	1283
EcoRII	CCWGG	9 cut(s)	81, 306, 712, 777, 1071, 1312, 1351, 1609, 2141
EcoT14I	CCWWGG	2 cut(s)	468, 809
EcoT22I	ATGCAT	1 cut(s)	1411
EcoT38I	GRGCYC	1 cut(s)	648
ErhI	CCWWGG	2 cut(s)	468, 809
Esp3I	CGTCTC	1 cut(s)	1454
FaeI	CATG	7 cut(s)	402, 541, 941, 1736, 2117, 2616, 2675
FaqI	GGGAC	1 cut(s)	2082
FatI	CATG	7 cut(s)	398, 537, 937, 1732, 2113, 2612, 2671
FauI	CCCGC	4 cut(s)	107, 515, 1272, 2446
FauNDI	CATATG	1 cut(s)	1819
FbaI	TGATCA	4 cut(s)	574, 1189, 1957, 2392
FblI	GTMKAC	1 cut(s)	2333
FokI	GGATG	5 cut(s)	737, 1279, 1947, 1961, 2209
FriOI	GRGCYC	1 cut(s)	648
FspBI	CTAG	5 cut(s)	447, 810, 1493, 1760, 2340
GlaI	GCGC	6 cut(s)	29, 31, 49, 178, 477, 609
GsuI	CTGGAG	5 cut(s)	8, 735, 1764, 1914, 2642
HaeIII	GGCC	3 cut(s)	80, 1931, 2140
HapII	CCGG	5 cut(s)	36, 69, 347, 1772, 1998
HgaI	GACGC	2 cut(s)	1026, 1816
HhaI	GCGC	6 cut(s)	30, 32, 50, 179, 478, 610
Hin1II	CATG	7 cut(s)	402, 541, 941, 1736, 2117, 2616, 2675
Hin6I	GCGC	6 cut(s)	28, 30, 48, 177, 476, 608
HinP1I	GCGC	6 cut(s)	28, 30, 48, 177, 476, 608
HincII	GTYRAC	2 cut(s)	453, 517
HindII	GTYRAC	2 cut(s)	453, 517
HindIII	AAGCTT	2 cut(s)	413, 2369
HpaII	CCGG	5 cut(s)	36, 69, 347, 1772, 1998
HphI	GGTGA	4 cut(s)	296, 1469, 1541, 2474
Hpy166II	GTNNAC	7 cut(s)	421, 453, 517, 1358, 1552, 2334, 2690
Hpy8I	GTNNAC	7 cut(s)	421, 453, 517, 1358, 1552, 2334, 2690
Hpy99I	CGWCG	1 cut(s)	466
HpyAV	CCTTC	7 cut(s)	128, 214, 1127, 1219, 1775, 2234, 2707
HpyCH4III	ACNGT	9 cut(s)	709, 1378, 1465, 1484, 1862, 2017, 2317, 2477, 2662
HpyCH4IV	ACGT	3 cut(s)	423, 654, 1588
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 45, 605, 832, 2119, 2264, 2408
HpySE526I	ACGT	3 cut(s)	423, 654, 1588
Hsp92II	CATG	7 cut(s)	402, 541, 941, 1736, 2117, 2616, 2675
HspAI	GCGC	6 cut(s)	28, 30, 48, 177, 476, 608
KpnI	GGTACC	1 cut(s)	1778
Ksp22I	TGATCA	4 cut(s)	574, 1189, 1957, 2392
KspI	CCGCGG	1 cut(s)	103
LguI	GCTCTTC	2 cut(s)	1080, 2409
LmnI	GCTCC	3 cut(s)	2000, 2399, 2623
Lsp1109I	GCAGC	7 cut(s)	532, 869, 1021, 2197, 2413, 2437, 2629
LweI	GCATC	3 cut(s)	1197, 1396, 1533
MabI	ACCWGGT	1 cut(s)	1312
MaeI	CTAG	5 cut(s)	447, 810, 1493, 1760, 2340
MaeII	ACGT	3 cut(s)	423, 654, 1588
MaeIII	GTNAC	6 cut(s)	483, 703, 925, 991, 1478, 2029
MbiI	CCGCTC	1 cut(s)	1671
MfeI	CAATTG	2 cut(s)	1452, 1935
MflI	RGATCY	5 cut(s)	144, 790, 1793, 1849, 2515
MhlI	GDGCHC	4 cut(s)	648, 1554, 2080, 2535
MlyI	GAGTC	4 cut(s)	145, 575, 1109, 2564
MmeI	TCCRAC	4 cut(s)	233, 314, 537, 651
Mph1103I	ATGCAT	1 cut(s)	1411
MseI	TTAA	9 cut(s)	921, 962, 1013, 1170, 1593, 1715, 1980, 2177, 2307
MslI	CAYNNNNRTG	2 cut(s)	1418, 1557
MspA1I	CMGCKG	1 cut(s)	102
MspCI	CTTAAG	1 cut(s)	1169
MspI	CCGG	5 cut(s)	36, 69, 347, 1772, 1998
MunI	CAATTG	2 cut(s)	1452, 1935
Mva1269I	GAATGC	1 cut(s)	2402
MvaI	CCWGG	9 cut(s)	83, 308, 714, 779, 1073, 1314, 1353, 1611, 2143
MvnI	CGCG	2 cut(s)	30, 102
MwoI	GCNNNNNNNGC	7 cut(s)	36, 45, 605, 832, 2119, 2264, 2408
NciI	CCSGG	3 cut(s)	348, 1772, 1999
NdeI	CATATG	1 cut(s)	1819
NlaIII	CATG	7 cut(s)	402, 541, 941, 1736, 2117, 2616, 2675
NlaIV	GGNNCC	6 cut(s)	241, 711, 1284, 1776, 1851, 2517
NmuCI	GTSAC	4 cut(s)	483, 703, 1478, 2029
NsiI	ATGCAT	1 cut(s)	1411
NspI	RCATGY	3 cut(s)	402, 2117, 2616
PaeI	GCATGC	1 cut(s)	2117
PagI	TCATGA	1 cut(s)	937
PauI	GCGCGC	1 cut(s)	28
PceI	AGGCCT	1 cut(s)	1931
PciI	ACATGT	1 cut(s)	2612
PciSI	GCTCTTC	2 cut(s)	1080, 2409
PctI	GAATGC	1 cut(s)	2402
PflFI	GACNNNGTC	1 cut(s)	1826
PfoI	TCCNGGA	3 cut(s)	712, 1609, 1997
PleI	GAGTC	4 cut(s)	145, 575, 1108, 2563
PpsI	GAGTC	4 cut(s)	145, 575, 1108, 2563
Ppu21I	YACGTR	1 cut(s)	655
PpuMI	RGGWCCY	1 cut(s)	1283
PscI	ACATGT	1 cut(s)	2612
PshBI	ATTAAT	1 cut(s)	1593
PsiI	TTATAA	1 cut(s)	771
Psp5II	RGGWCCY	1 cut(s)	1283
Psp6I	CCWGG	9 cut(s)	81, 306, 712, 777, 1071, 1312, 1351, 1609, 2141
PspGI	CCWGG	9 cut(s)	81, 306, 712, 777, 1071, 1312, 1351, 1609, 2141
PspN4I	GGNNCC	6 cut(s)	241, 711, 1284, 1776, 1851, 2517
PspPI	GGNCC	5 cut(s)	79, 240, 728, 1283, 2139
PspPPI	RGGWCCY	1 cut(s)	1283
PstI	CTGCAG	1 cut(s)	832
PstNI	CAGNNNCTG	1 cut(s)	2558
PsuI	RGATCY	5 cut(s)	144, 790, 1793, 1849, 2515
PsyI	GACNNNGTC	1 cut(s)	1826
PteI	GCGCGC	1 cut(s)	28
RsaI	GTAC	6 cut(s)	5, 1064, 1359, 1776, 2659, 2689
RsaNI	GTAC	6 cut(s)	4, 1063, 1358, 1775, 2658, 2688
RseI	CAYNNNNRTG	2 cut(s)	1418, 1557
SacII	CCGCGG	1 cut(s)	103
SapI	GCTCTTC	2 cut(s)	1080, 2409
SaqAI	TTAA	9 cut(s)	921, 962, 1013, 1170, 1593, 1715, 1980, 2177, 2307
Sau96I	GGNCC	5 cut(s)	79, 240, 728, 1283, 2139
SchI	GAGTC	4 cut(s)	145, 575, 1109, 2564
SduI	GDGCHC	4 cut(s)	648, 1554, 2080, 2535
SexAI	ACCWGGT	1 cut(s)	1312
SfaNI	GCATC	3 cut(s)	1197, 1396, 1533
SfcI	CTRYAG	2 cut(s)	10, 828
Sfr303I	CCGCGG	1 cut(s)	103
SgrBI	CCGCGG	1 cut(s)	103
SinI	GGWCC	3 cut(s)	240, 728, 1283
SmiMI	CAYNNNNRTG	2 cut(s)	1418, 1557
SmlI	CTYRAG	3 cut(s)	266, 1169, 1919
SmoI	CTYRAG	3 cut(s)	266, 1169, 1919
SphI	GCATGC	1 cut(s)	2117
SseBI	AGGCCT	1 cut(s)	1931
SspI	AATATT	1 cut(s)	1263
SspMI	CTAG	5 cut(s)	447, 810, 1493, 1760, 2340
StuI	AGGCCT	1 cut(s)	1931
StyI	CCWWGG	2 cut(s)	468, 809
TaaI	ACNGT	9 cut(s)	709, 1378, 1465, 1484, 1862, 2017, 2317, 2477, 2662
TaiI	ACGT	3 cut(s)	426, 657, 1591
TaqI	TCGA	4 cut(s)	114, 503, 659, 2059
TatI	WGTACW	1 cut(s)	2657
TauI	GCSGC	3 cut(s)	75, 105, 599
Tru1I	TTAA	9 cut(s)	921, 962, 1013, 1170, 1593, 1715, 1980, 2177, 2307
Tru9I	TTAA	9 cut(s)	921, 962, 1013, 1170, 1593, 1715, 1980, 2177, 2307
TscAI	CASTG	6 cut(s)	214, 1333, 1489, 1671, 2331, 2667
TseFI	GTSAC	4 cut(s)	483, 703, 1478, 2029
TseI	GCWGC	7 cut(s)	545, 857, 1034, 2210, 2426, 2450, 2642
Tsp45I	GTSAC	4 cut(s)	483, 703, 1478, 2029
TspDTI	ATGAA	9 cut(s)	1095, 1238, 1281, 1413, 1611, 1749, 1806, 2211, 2325
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	6 cut(s)	214, 1333, 1489, 1671, 2331, 2667
Tth111I	GACNNNGTC	1 cut(s)	1826
Vha464I	CTTAAG	1 cut(s)	1169
VneI	GTGCAC	1 cut(s)	1550
VpaK11BI	GGWCC	3 cut(s)	240, 728, 1283
VspI	ATTAAT	1 cut(s)	1593
XapI	RAATTY	5 cut(s)	168, 757, 1400, 1869, 2665
XbaI	TCTAGA	2 cut(s)	1492, 1759
XceI	RCATGY	3 cut(s)	402, 2117, 2616
XmaJI	CCTAGG	1 cut(s)	809
XmiI	GTMKAC	1 cut(s)	2333
XspI	CTAG	5 cut(s)	447, 810, 1493, 1760, 2340
Zsp2I	ATGCAT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.