MD01G1136600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015032
Physical Location & Seq
Reverse (-)
24584839 .. 24586119
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1136600.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGCCCATACTCCTCCCCCTCCTTCTTCTTCAACTCCTCACCACCCTCTGTTTCTCTGAGCCAAAACCAGAAACTCTAATCCTGCCCCTCAAAACACAGACCCTCCCACACGGGTCCCTCCCAAAATCCACCAACAAGCTCTCCTTCCACCACAACGTCACCCTCACAATCTCCCTCTCCGTCGGCTCTCCGCCGCAGCAAGTCACCATGGTCCTCGACACAGGCAGCGAGCTTTCGTGGCTTCGCTGCAAAAAAGCCCCCAACTTCAACTCTGTCTTCAACCCACTCGCCTCCAAGTCCTACTCCCCAGTCCCCTGCTCCTCCCCCGTCTGCCGGACCCGAACCCGCGACTTCCCCACACCCGTTTCTTGCGACCCGAAAAAGCTCTGCCACTCCATTCTCTCCTACGTCGACGCCTCCTCCATCGAAGGTAACCTCGCATGGGAAACTTTCAATCTCGGGTCGTCCGCCCAACCCGGTACCATATTCGGGTGCATGGATTCCGGGTCCAGTTCGAACGCTGAAGAGGACGCGAAGACAACCGGGTTAATGGGCATGAACCGCGGTTCATTGTCCTTCGTTACACAGATGGGATTTCCGAAATTTTCCTACTGCATATCGGGTCGCGACTCGTCCGGTGTTTTGCTTTTCGGGGAAGCGAAATTCGATTGGCTCAAACCGTTAAATTACACCCCACTGGTTCATATTTCAACCCCATTGCCGTACTTCGACCGGGTAGCGTACACGGTCCAGCTGGAGGGGATCCGGGTTGGAGGGAAGTTGTTACCGCTCCCGAAGTCCGTTTTCGTACCGGACCATTCCGGGGCAGGTCAAACAATGGTCGACTCGGGAACCCAGTTCACGTTCCTACTCGGTCCGGTTTACACCGCGCTGAAAAAGGAATTCACTCAGCAGACCAAACCGGTTCTTAACATTTTGAACGACCCGAATTTTGTGTTCCAAGGAGCGATCGATTTGTGTTTCCAAGTCCCGACGAACCGGCCGAGTCTGCCCGCGTTGCCGACAGTAACGCTGATGTTTCGGGGGGCCGAGATGAGCGTCTCGGGGGAAAGGCTGCTGTATCGGGTGCCGGGAATGGTGAGGGGCGGGAATCAGGTGTATTGCTTCACATATGGGAACTCCGACTTGCTGGGCATAGAGGCGTTTGTGATTGGTCACTATCATCAGCAGAACGTGTGGATGGAGTTTGATTTGGAGAAGTCTAGGGTAGGAGTGGCTGAGGTTAGGTGTGAACTTGCAAGTCAAAAGCTTGGTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

427

Amino Acids

46.65

Weight (kDa)

8.23

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 57 - 218 3.5e-37 Xylanase inhibitor N-terminal
Asp PF00026 58 - 415 6.3e-15 Eukaryotic aspartyl protease
TAXi_C PF14541 247 - 411 3.2e-33 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39710
fragaria_vesca FvH4_7g22840
malus_domestica MD01G1136600.v1.1 MD07G1201600.v1.1
prunus_persica Prupe.2G238200_v2.0.a1
pyrus_communis pycom01g15730 pycom07g18860
rosa_chinensis RchiOBHm_Chr1g0368001
rosa_laevigata RLG00000027248
rosa_roxburghii Rroxscaffold_4G00288960
rosa_rugosa Rorug01G0338400
rosa_samantha Rh1AG345100 Rh1BG307400 Rh1CG323000 Rh1DG339500
rosa_wichuraiana Rw1G030710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 818
Acc65I GGTACC 1 cut(s) 479
AccB1I GGYRCC 2 cut(s) 479, 1087
AccBSI CCGCTC 1 cut(s) 790
AccI GTMKAC 2 cut(s) 411, 843
AccII CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
AclWI GGATC 2 cut(s) 757, 770
AcoI YGGCCR 1 cut(s) 1001
AcsI RAATTY 4 cut(s) 602, 662, 902, 949
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 4 cut(s) 481, 725, 743, 810
AfiI CCNNNNNNNGG 5 cut(s) 333, 442, 489, 757, 823
AflIII ACRYGT 1 cut(s) 1194
AgeI ACCGGT 1 cut(s) 922
AgsI TTSAA 6 cut(s) 32, 268, 280, 454, 711, 940
AluBI AGCT 5 cut(s) 139, 232, 385, 754, 1270
AluI AGCT 5 cut(s) 139, 232, 385, 754, 1270
Alw26I GTCTC 1 cut(s) 1066
AlwI GGATC 2 cut(s) 757, 770
Ama87I CYCGRG 3 cut(s) 458, 847, 1063
AoxI GGCC 2 cut(s) 1001, 1047
ApeKI GCWGC 4 cut(s) 196, 225, 246, 1075
ApoI RAATTY 4 cut(s) 602, 662, 902, 949
ArsI GACNNNNNNTTYG 3 cut(s) 935, 967, 1258
AsiGI ACCGGT 1 cut(s) 922
Asp718I GGTACC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 892
AspS9I GGNCC 8 cut(s) 114, 211, 336, 507, 748, 814, 875, 1047
AsuC2I CCSGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
AsuHPI GGTGA 4 cut(s) 31, 151, 196, 1111
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 3 cut(s) 458, 847, 1063
AvaII GGWCC 7 cut(s) 114, 211, 336, 507, 748, 814, 875
BamHI GGATCC 1 cut(s) 762
BanI GGYRCC 2 cut(s) 479, 1087
BbsI GAAGAC 2 cut(s) 268, 542
BbvCI CCTCAGC 1 cut(s) 1239
BbvI GCAGC 4 cut(s) 208, 233, 237, 1062
BccI CCATC 3 cut(s) 431, 583, 1195
BceAI ACGGC 1 cut(s) 706
BcnI CCSGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
BcoDI GTCTC 1 cut(s) 1066
BfaI CTAG 2 cut(s) 1224, 1279
BfuAI ACCTGC 1 cut(s) 818
BisI GCNGC 5 cut(s) 194, 197, 226, 247, 1076
BlsI GCNGC 5 cut(s) 195, 198, 227, 248, 1077
Bme1390I CCNGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
Bme18I GGWCC 7 cut(s) 114, 211, 336, 507, 748, 814, 875
BmeT110I CYCGRG 3 cut(s) 458, 847, 1063
BmgT120I GGNCC 8 cut(s) 114, 211, 336, 507, 748, 814, 875, 1047
BmiI GGNNCC 9 cut(s) 115, 116, 338, 481, 508, 764, 853, 1048, 1089
BmrFI CCNGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
BmrI ACTGGG 2 cut(s) 302, 850
BmuI ACTGGG 2 cut(s) 302, 850
BpiI GAAGAC 2 cut(s) 268, 542
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 1 cut(s) 1239
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
Bsa29I ATCGAT 1 cut(s) 972
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 4 cut(s) 207, 562, 822, 961
BsaWI WCCGGW 4 cut(s) 635, 811, 877, 922
BsaXI ACNNNNNCTCC 6 cut(s) 87, 117, 125, 155, 404, 434
Bsc4I CCNNNNNNNGG 5 cut(s) 333, 442, 489, 757, 823
Bse118I RCCGGY 2 cut(s) 922, 999
Bse1I ACTGG 4 cut(s) 308, 510, 702, 856
Bse3DI GCAATG 1 cut(s) 716
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 4 cut(s) 207, 562, 822, 961
BseGI GGATG 1 cut(s) 1206
BseLI CCNNNNNNNGG 5 cut(s) 333, 442, 489, 757, 823
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 3 cut(s) 48, 923, 1230
BseNI ACTGG 4 cut(s) 308, 510, 702, 856
BseRI GAGGAG 3 cut(s) 26, 310, 409
BseX3I CGGCCG 1 cut(s) 1001
BseXI GCAGC 4 cut(s) 208, 233, 237, 1062
BseYI CCCAGC 1 cut(s) 1150
Bsh1236I CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
Bsh1285I CGRYCG 3 cut(s) 733, 972, 1004
BshFI GGCC 2 cut(s) 1003, 1049
BshNI GGYRCC 2 cut(s) 479, 1087
BshTI ACCGGT 1 cut(s) 922
BshVI ATCGAT 1 cut(s) 972
BsiEI CGRYCG 3 cut(s) 733, 972, 1004
BsiHKCI CYCGRG 3 cut(s) 458, 847, 1063
BslFI GGGAC 3 cut(s) 100, 296, 974
BslI CCNNNNNNNGG 5 cut(s) 333, 442, 489, 757, 823
BsmAI GTCTC 1 cut(s) 1066
BsmBI CGTCTC 1 cut(s) 1066
BsmFI GGGAC 3 cut(s) 100, 296, 974
BsnI GGCC 2 cut(s) 1003, 1049
BsoBI CYCGRG 3 cut(s) 458, 847, 1063
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 2 cut(s) 762, 969
Bsp19I CCATGG 1 cut(s) 207
Bsp68I TCGCGA 1 cut(s) 627
BspANI GGCC 2 cut(s) 1003, 1049
BspCNI CTCAG 3 cut(s) 49, 922, 1231
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
BspLI GGNNCC 9 cut(s) 115, 116, 338, 481, 508, 764, 853, 1048, 1089
BspMI ACCTGC 1 cut(s) 818
BspPI GGATC 2 cut(s) 757, 770
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 2 cut(s) 479, 1087
BsrBI CCGCTC 1 cut(s) 790
BsrDI GCAATG 1 cut(s) 716
BsrFI RCCGGY 2 cut(s) 922, 999
BsrI ACTGG 4 cut(s) 308, 510, 702, 856
BssAI RCCGGY 2 cut(s) 922, 999
BssECI CCNNGG 4 cut(s) 207, 562, 822, 961
BssMI GATC 2 cut(s) 762, 969
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 207, 961
Bst4CI ACNGT 3 cut(s) 681, 748, 1027
Bst6I CTCTTC 1 cut(s) 519
BstACI GRCGYC 1 cut(s) 414
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 230, 1014
BstDEI CTNAG 3 cut(s) 57, 909, 1239
BstDSI CCRYGG 2 cut(s) 207, 562
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 1 cut(s) 1206
BstFNI CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
BstHHI GCGC 1 cut(s) 892
BstKTI GATC 2 cut(s) 765, 972
BstMAI GTCTC 1 cut(s) 1066
BstMBI GATC 2 cut(s) 762, 969
BstMCI CGRYCG 3 cut(s) 733, 972, 1004
BstMWI GCNNNNNNNGC 4 cut(s) 238, 561, 1009, 1018
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 7 cut(s) 475, 503, 542, 732, 765, 821, 1090
BstUI CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
BstV1I GCAGC 4 cut(s) 208, 233, 237, 1062
BstV2I GAAGAC 2 cut(s) 268, 542
BstX2I RGATCY 1 cut(s) 762
BstYI RGATCY 1 cut(s) 762
BstZI CGGCCG 1 cut(s) 1001
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 2 cut(s) 1003, 1049
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 2 cut(s) 207, 562
BtsCI GGATG 1 cut(s) 1206
BtsIMutI CAGTG 1 cut(s) 695
BtuMI TCGCGA 1 cut(s) 627
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 2 cut(s) 230, 1014
CfoI GCGC 1 cut(s) 892
Cfr10I RCCGGY 2 cut(s) 922, 999
Cfr13I GGNCC 8 cut(s) 114, 211, 336, 507, 748, 814, 875, 1047
Cfr42I CCGCGG 1 cut(s) 565
ClaI ATCGAT 1 cut(s) 972
CpoI CGGWCCG 1 cut(s) 875
CseI GACGC 3 cut(s) 422, 539, 1048
Csp6I GTAC 4 cut(s) 480, 724, 742, 809
CspAI ACCGGT 1 cut(s) 922
CspI CGGWCCG 1 cut(s) 875
CviAII CATG 4 cut(s) 208, 441, 496, 556
CviQI GTAC 4 cut(s) 480, 724, 742, 809
DdeI CTNAG 3 cut(s) 57, 909, 1239
DpnI GATC 2 cut(s) 764, 971
DpnII GATC 2 cut(s) 762, 969
EaeI YGGCCR 1 cut(s) 1001
EagI CGGCCG 1 cut(s) 1001
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
EciI GGCGGA 2 cut(s) 180, 457
EclXI CGGCCG 1 cut(s) 1001
Eco130I CCWWGG 2 cut(s) 207, 961
Eco47I GGWCC 7 cut(s) 114, 211, 336, 507, 748, 814, 875
Eco52I CGGCCG 1 cut(s) 1001
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 3 cut(s) 458, 847, 1063
Eco91I GGTNACC 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 114
EcoO65I GGTNACC 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 902
EcoT14I CCWWGG 2 cut(s) 207, 961
ErhI CCWWGG 2 cut(s) 207, 961
Esp3I CGTCTC 1 cut(s) 1066
FaeI CATG 4 cut(s) 211, 444, 499, 559
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 3 cut(s) 100, 296, 974
FatI CATG 4 cut(s) 207, 440, 495, 555
FauI CCCGC 3 cut(s) 353, 1021, 1100
FauNDI CATATG 1 cut(s) 1132
FblI GTMKAC 2 cut(s) 411, 843
Fnu4HI GCNGC 5 cut(s) 194, 197, 226, 247, 1076
FokI GGATG 1 cut(s) 1213
Fsp4HI GCNGC 5 cut(s) 194, 197, 226, 247, 1076
FspBI CTAG 2 cut(s) 1224, 1279
GlaI GCGC 1 cut(s) 891
GluI GCNGC 5 cut(s) 194, 197, 226, 247, 1076
GsaI CCCAGC 1 cut(s) 1154
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 2 cut(s) 1003, 1049
HgaI GACGC 3 cut(s) 422, 539, 1048
HhaI GCGC 1 cut(s) 892
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 4 cut(s) 211, 444, 499, 559
Hin6I GCGC 1 cut(s) 890
HinP1I GCGC 1 cut(s) 890
HincII GTYRAC 2 cut(s) 412, 844
HindII GTYRAC 2 cut(s) 412, 844
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 5 cut(s) 500, 629, 845, 1006, 1111
HphI GGTGA 4 cut(s) 31, 151, 196, 1111
Hpy166II GTNNAC 6 cut(s) 412, 744, 844, 861, 883, 1253
Hpy188I TCNGA 3 cut(s) 58, 600, 1144
Hpy188III TCNNGA 4 cut(s) 626, 793, 849, 991
Hpy8I GTNNAC 6 cut(s) 412, 744, 844, 861, 883, 1253
Hpy99I CGWCG 4 cut(s) 185, 413, 416, 997
HpyAV CCTTC 4 cut(s) 32, 154, 422, 586
HpyCH4III ACNGT 3 cut(s) 681, 748, 1027
HpyCH4IV ACGT 4 cut(s) 156, 408, 863, 1194
HpyCH4V TGCA 4 cut(s) 249, 495, 615, 1259
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 561, 1009, 1018
HpyF3I CTNAG 3 cut(s) 57, 909, 1239
HpySE526I ACGT 4 cut(s) 156, 408, 863, 1194
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 4 cut(s) 211, 444, 499, 559
HspAI GCGC 1 cut(s) 890
KflI GGGWCCC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 483
KspI CCGCGG 1 cut(s) 565
Kzo9I GATC 2 cut(s) 762, 969
LmnI GCTCC 3 cut(s) 323, 795, 965
Lsp1109I GCAGC 4 cut(s) 208, 233, 237, 1062
MaeI CTAG 2 cut(s) 1224, 1279
MaeII ACGT 4 cut(s) 156, 408, 863, 1194
MaeIII GTNAC 7 cut(s) 157, 202, 431, 580, 783, 1027, 1175
MalI GATC 2 cut(s) 764, 971
MbiI CCGCTC 1 cut(s) 790
MboI GATC 2 cut(s) 762, 969
MboII GAAGA 5 cut(s) 17, 20, 268, 536, 547
MflI RGATCY 1 cut(s) 762
MluCI AATT 5 cut(s) 602, 662, 685, 902, 949
MlyI GAGTC 3 cut(s) 623, 839, 1015
MmeI TCCRAC 2 cut(s) 751, 1167
MseI TTAA 3 cut(s) 548, 683, 930
MspA1I CMGCKG 2 cut(s) 564, 754
MspR9I CCNGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
MvnI CGCG 6 cut(s) 348, 533, 564, 627, 890, 1016
MwoI GCNNNNNNNGC 4 cut(s) 238, 561, 1009, 1018
NciI CCSGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 1132
NdeII GATC 2 cut(s) 762, 969
NlaIII CATG 4 cut(s) 211, 444, 499, 559
NlaIV GGNNCC 9 cut(s) 115, 116, 338, 481, 508, 764, 853, 1048, 1089
NmeAIII GCCGAG 2 cut(s) 1029, 1075
NmuCI GTSAC 3 cut(s) 157, 202, 1175
NruI TCGCGA 1 cut(s) 627
NspV TTCGAA 1 cut(s) 515
PcsI WCGNNNNNNNCGW 1 cut(s) 1001
PfeI GAWTC 2 cut(s) 500, 1111
PinAI ACCGGT 1 cut(s) 922
PkrI GCNGC 5 cut(s) 195, 198, 227, 248, 1077
Ple19I CGATCG 1 cut(s) 972
PleI GAGTC 3 cut(s) 623, 839, 1014
PpsI GAGTC 3 cut(s) 623, 839, 1014
PpuMI RGGWCCY 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 114
PspEI GGTNACC 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 1150
PspN4I GGNNCC 9 cut(s) 115, 116, 338, 481, 508, 764, 853, 1048, 1089
PspPI GGNCC 8 cut(s) 114, 211, 336, 507, 748, 814, 875, 1047
PspPPI RGGWCCY 1 cut(s) 114
PsuI RGATCY 1 cut(s) 762
PvuI CGATCG 1 cut(s) 972
PvuII CAGCTG 1 cut(s) 754
RruI TCGCGA 1 cut(s) 627
RsaI GTAC 4 cut(s) 481, 725, 743, 810
RsaNI GTAC 4 cut(s) 480, 724, 742, 809
Rsr2I CGGWCCG 1 cut(s) 875
RsrII CGGWCCG 1 cut(s) 875
SacII CCGCGG 1 cut(s) 565
SalI GTCGAC 2 cut(s) 410, 842
SaqAI TTAA 3 cut(s) 548, 683, 930
SatI GCNGC 5 cut(s) 194, 197, 226, 247, 1076
Sau3AI GATC 2 cut(s) 762, 969
Sau96I GGNCC 8 cut(s) 114, 211, 336, 507, 748, 814, 875, 1047
SchI GAGTC 3 cut(s) 623, 839, 1015
ScrFI CCNGG 7 cut(s) 477, 505, 544, 734, 767, 823, 1092
Sfr303I CCGCGG 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 515
SgrBI CCGCGG 1 cut(s) 565
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 7 cut(s) 114, 211, 336, 507, 748, 814, 875
Sse9I AATT 5 cut(s) 602, 662, 685, 902, 949
SspMI CTAG 2 cut(s) 1224, 1279
StyD4I CCNGG 7 cut(s) 475, 503, 542, 732, 765, 821, 1090
StyI CCWWGG 2 cut(s) 207, 961
TaaI ACNGT 3 cut(s) 681, 748, 1027
TaiI ACGT 4 cut(s) 159, 411, 866, 1197
TaqI TCGA 8 cut(s) 216, 411, 426, 515, 666, 729, 843, 972
TaqII GACCGA 1 cut(s) 863
TasI AATT 5 cut(s) 602, 662, 685, 902, 949
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 2 cut(s) 500, 1111
Tru1I TTAA 3 cut(s) 548, 683, 930
Tru9I TTAA 3 cut(s) 548, 683, 930
TscAI CASTG 1 cut(s) 702
TseFI GTSAC 3 cut(s) 157, 202, 1175
TseI GCWGC 4 cut(s) 196, 225, 246, 1075
Tsp45I GTSAC 3 cut(s) 157, 202, 1175
TspDTI ATGAA 3 cut(s) 558, 572, 692
TspGWI ACGGA 2 cut(s) 169, 790
TspRI CASTG 1 cut(s) 702
VpaK11BI GGWCC 7 cut(s) 114, 211, 336, 507, 748, 814, 875
XapI RAATTY 4 cut(s) 602, 662, 902, 949
XmiI GTMKAC 2 cut(s) 411, 843
XspI CTAG 2 cut(s) 1224, 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.