Rh1AG345100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015032
Physical Location & Seq
Reverse (-)
58117746 .. 58120409
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG345100.1

Sequence Viewer

Length: 1299 bp
ATGCCACTTCTTCTTCTTCTTCTTCATCTTCTCCTTCATTTTTTCACTTACTACAGTCTCTGCTTCTCTTCTCCACTACCTAAAACCCTCATTCTACCTCTCAAAACCCAGACACTCCCACATGGGTCGATCCCAAAACCCTCAAACAAGCTCTCATTCCACCACAACGTCACCCTAACAATCTCCCTCACAGTCGGGTCGCCCCCACAGAACGTTACCATGGTCCTCGACACAGGCAGTGAACTCTCCTGGCTCCACTGTAAGAAAACCCCGACCCTCACCAACGTCTTCAACCCACTCGCCTCCAAATCCTACATCCCCGTCTCCTGCTCCACCCCCACTTGCCGCACCCGGACCCGGGACCTATCCATACCCGTTTCCTGCGACCCGAAAAAGCTCTGCCACGCCATTCTCTCCTACGCCGACGCGTCCTCCCTGGAAGGCAACCTCGCCTCCGACACTTTCCAACTCGGGTCTTCGGGTCAACCCGGGACCGTATTCGGGTGCATGGACTCCGGGTTCAGTTCCAACTCCGAAGAGGACGCCAAGACAACCGGGTTAATGGGCATGAACCGGGGATCCTTATCCTTCGTAACCCAAATGGGCTTCCCGAGATTCTCGTACTGCATATCGGGCCGTGACTCCTCCGGGGTCTTGCTCTTCGGCGACGCCAACTTCGCGTGGCTCGGCCCGTTAAACTACACGCCGTTAGTCCAAATGTCGACGCCGTTACCGTACTTCGACCGGGTCGCGTACACGGTCCAGCTCGACGGGATCCGGGTCTCCGGGAAAGTACTCCCCCTCCCGAAATCCGCGTTCGTACCCGACCACACCGGGGCGGGTCAGACAATGGTGGACTCGGGCACCCAGTTCACGTTTCTTCTCGGGCCCGTTTACACCGCTTTGAAAAACGAGTTCGTTCTCCAGACCAAACCGGTTCTGAAGCTCCTGGACGACCCGAACTTCGTTTTCCAAGGCGCGATGGATTTGTGTTTTCTGGTGCCGATGAACCGGCCGGGTCTGCCGGTTCTGCCAGCTGTGACGATGATGTTTAAGGGAGCAGAGATGAGCGTAACGGGGGAAAGGCTGCTGTATCGGGTACCGGGAATGGTGAGGGGGAATAATCAGGTGTATTGCTTCACGTTCGGGAACTCCGACTTGCTGGGCGTAGAGGCGTTTGTGATTGGTCACTATCACCAGCAGAACGTGTGGATGGAGTTTGATCTGGTGAAGTCTAGGATGGGAGTGGCCGAGGTTAGGTGTGATCTCGCAAGTCAAAGGCTAGGTTTGGGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

432

Amino Acids

47.2

Weight (kDa)

8.24

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 61 - 223 2.4e-36 Xylanase inhibitor N-terminal
Asp PF00026 62 - 419 4.3e-14 Eukaryotic aspartyl protease
TAXi_C PF14541 251 - 415 6.4e-31 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39710
fragaria_vesca FvH4_7g22840
malus_domestica MD01G1136600.v1.1 MD07G1201600.v1.1
prunus_persica Prupe.2G238200_v2.0.a1
pyrus_communis pycom01g15730 pycom07g18860
rosa_chinensis RchiOBHm_Chr1g0368001
rosa_laevigata RLG00000027248
rosa_roxburghii Rroxscaffold_4G00288960
rosa_rugosa Rorug01G0338400
rosa_samantha Rh1AG345100 Rh1BG307400 Rh1CG323000 Rh1DG339500
rosa_wichuraiana Rw1G030710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1099, 1292
AccB1I GGYRCC 4 cut(s) 863, 1000, 1099, 1292
AccI GTMKAC 1 cut(s) 722
AccII CGCG 5 cut(s) 428, 680, 752, 815, 980
AciI CCGC 4 cut(s) 346, 813, 839, 900
AclI AACGTT 1 cut(s) 213
AclWI GGATC 5 cut(s) 124, 573, 586, 769, 782
AcoI YGGCCR 2 cut(s) 1013, 1248
AcuI CTGAAG 1 cut(s) 962
AcyI GRCGYC 3 cut(s) 543, 669, 725
AfaI GTAC 7 cut(s) 623, 737, 755, 795, 822, 1101, 1294
AfiI CCNNNNNNNGG 5 cut(s) 357, 358, 501, 835, 1257
AflIII ACRYGT 2 cut(s) 426, 1206
AgeI ACCGGT 1 cut(s) 934
AgsI TTSAA 2 cut(s) 292, 907
AjnI CCWGG 3 cut(s) 248, 435, 948
AloI GAACNNNNNNTCC 4 cut(s) 503, 535, 563, 595
AluBI AGCT 5 cut(s) 151, 397, 766, 946, 1037
AluI AGCT 5 cut(s) 151, 397, 766, 946, 1037
Alw26I GTCTC 3 cut(s) 62, 328, 787
AlwI GGATC 5 cut(s) 124, 573, 586, 769, 782
AlwNI CAGNNNCTG 1 cut(s) 60
Ama87I CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
AoxI GGCC 5 cut(s) 634, 688, 887, 1013, 1248
ApaI GGGCCC 1 cut(s) 891
ApeKI GCWGC 1 cut(s) 1087
ArsI GACNNNNNNTTYG 4 cut(s) 659, 691, 947, 979
AsiGI ACCGGT 1 cut(s) 934
Asp718I GGTACC 2 cut(s) 1099, 1292
AspLEI GCGC 1 cut(s) 980
AspS9I GGNCC 9 cut(s) 223, 354, 361, 492, 634, 689, 760, 887, 888
AsuHPI GGTGA 5 cut(s) 163, 271, 1123, 1187, 1240
AvaI CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
AvaII GGWCC 5 cut(s) 223, 354, 361, 492, 760
BaeGI GKGCMC 2 cut(s) 866, 891
BamHI GGATCC 2 cut(s) 578, 774
BanI GGYRCC 4 cut(s) 863, 1000, 1099, 1292
BanII GRGCYC 1 cut(s) 891
BbsI GAAGAC 2 cut(s) 280, 468
BbvI GCAGC 1 cut(s) 1074
BccI CCATC 3 cut(s) 976, 1207, 1234
BceAI ACGGC 3 cut(s) 621, 691, 712
BciT130I CCWGG 3 cut(s) 250, 437, 950
BcoDI GTCTC 3 cut(s) 62, 328, 787
BfaI CTAG 3 cut(s) 1236, 1283, 1297
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 2 cut(s) 346, 1088
BlsI GCNGC 2 cut(s) 347, 1089
BmcAI AGTACT 1 cut(s) 795
Bme18I GGWCC 5 cut(s) 223, 354, 361, 492, 760
BmeT110I CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
BmgT120I GGNCC 9 cut(s) 223, 354, 361, 492, 634, 689, 760, 887, 888
BmrI ACTGGG 1 cut(s) 862
BmuI ACTGGG 1 cut(s) 862
BpiI GAAGAC 2 cut(s) 280, 468
BpmI CTGGAG 1 cut(s) 908
BsaBI GATNNNNATC 1 cut(s) 583
BsaHI GRCGYC 3 cut(s) 543, 669, 725
BsaI GGTCTC 1 cut(s) 787
BsaJI CCNNGG 9 cut(s) 219, 357, 435, 488, 574, 648, 834, 973, 1251
BsaWI WCCGGW 1 cut(s) 934
BsaXI ACNNNNNCTCC 6 cut(s) 416, 437, 446, 467, 786, 816
Bsc4I CCNNNNNNNGG 5 cut(s) 357, 358, 501, 835, 1257
Bse118I RCCGGY 3 cut(s) 934, 1011, 1024
Bse1I ACTGG 1 cut(s) 868
Bse8I GATNNNNATC 1 cut(s) 583
BseBI CCWGG 3 cut(s) 250, 437, 950
BseDI CCNNGG 9 cut(s) 219, 357, 435, 488, 574, 648, 834, 973, 1251
BseGI GGATG 3 cut(s) 315, 1218, 1245
BseJI GATNNNNATC 1 cut(s) 583
BseLI CCNNNNNNNGG 5 cut(s) 357, 358, 501, 835, 1257
BseNI ACTGG 1 cut(s) 868
BseRI GAGGAG 1 cut(s) 634
BseSI GKGCMC 2 cut(s) 866, 891
BseX3I CGGCCG 1 cut(s) 1013
BseXI GCAGC 1 cut(s) 1074
BseYI CCCAGC 1 cut(s) 1162
Bsh1236I CGCG 5 cut(s) 428, 680, 752, 815, 980
Bsh1285I CGRYCG 2 cut(s) 745, 1016
BshFI GGCC 5 cut(s) 636, 690, 889, 1015, 1250
BshNI GGYRCC 4 cut(s) 863, 1000, 1099, 1292
BshTI ACCGGT 1 cut(s) 934
BsiEI CGRYCG 2 cut(s) 745, 1016
BsiHKCI CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
BslFI GGGAC 2 cut(s) 374, 505
BslI CCNNNNNNNGG 5 cut(s) 357, 358, 501, 835, 1257
BsmAI GTCTC 3 cut(s) 62, 328, 787
BsmBI CGTCTC 1 cut(s) 328
BsmFI GGGAC 2 cut(s) 374, 505
BsnI GGCC 5 cut(s) 636, 690, 889, 1015, 1250
Bso31I GGTCTC 1 cut(s) 787
BsoBI CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
Bsp120I GGGCCC 1 cut(s) 887
Bsp1286I GDGCHC 2 cut(s) 866, 891
Bsp143I GATC 5 cut(s) 129, 578, 774, 1222, 1264
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 4 cut(s) 346, 813, 839, 900
BspANI GGCC 5 cut(s) 636, 690, 889, 1015, 1250
BspFNI CGCG 5 cut(s) 428, 680, 752, 815, 980
BspPI GGATC 5 cut(s) 124, 573, 586, 769, 782
BspQI GCTCTTC 1 cut(s) 665
BspT107I GGYRCC 4 cut(s) 863, 1000, 1099, 1292
BspTNI GGTCTC 1 cut(s) 787
BsrFI RCCGGY 3 cut(s) 934, 1011, 1024
BsrI ACTGG 1 cut(s) 868
BssAI RCCGGY 3 cut(s) 934, 1011, 1024
BssECI CCNNGG 9 cut(s) 219, 357, 435, 488, 574, 648, 834, 973, 1251
BssMI GATC 5 cut(s) 129, 578, 774, 1222, 1264
BssNI GRCGYC 3 cut(s) 543, 669, 725
BssT1I CCWWGG 2 cut(s) 219, 973
Bst2UI CCWGG 3 cut(s) 250, 437, 950
Bst4CI ACNGT 6 cut(s) 56, 193, 260, 496, 735, 760
Bst6I CTCTTC 3 cut(s) 73, 531, 665
BstACI GRCGYC 3 cut(s) 543, 669, 725
BstC8I GCNNGC 1 cut(s) 1035
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 3 cut(s) 315, 1218, 1245
BstFNI CGCG 5 cut(s) 428, 680, 752, 815, 980
BstHHI GCGC 1 cut(s) 980
BstKTI GATC 5 cut(s) 132, 581, 777, 1225, 1267
BstMAI GTCTC 3 cut(s) 62, 328, 787
BstMBI GATC 5 cut(s) 129, 578, 774, 1222, 1264
BstMCI CGRYCG 2 cut(s) 745, 1016
BstMWI GCNNNNNNNGC 4 cut(s) 633, 677, 1021, 1030
BstNI CCWGG 3 cut(s) 250, 437, 950
BstSFI CTRYAG 1 cut(s) 52
BstSLI GKGCMC 2 cut(s) 866, 891
BstUI CGCG 5 cut(s) 428, 680, 752, 815, 980
BstV1I GCAGC 1 cut(s) 1074
BstV2I GAAGAC 2 cut(s) 280, 468
BstX2I RGATCY 2 cut(s) 578, 774
BstYI RGATCY 2 cut(s) 578, 774
BstZI CGGCCG 1 cut(s) 1013
BsuRI GGCC 5 cut(s) 636, 690, 889, 1015, 1250
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 3 cut(s) 315, 1218, 1245
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 244, 256
Cac8I GCNNGC 1 cut(s) 1035
CaiI CAGNNNCTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 980
Cfr10I RCCGGY 3 cut(s) 934, 1011, 1024
Cfr13I GGNCC 9 cut(s) 223, 354, 361, 492, 634, 689, 760, 887, 888
Cfr9I CCCGGG 2 cut(s) 357, 488
CseI GACGC 5 cut(s) 417, 434, 551, 677, 733
Csp6I GTAC 7 cut(s) 622, 736, 754, 794, 821, 1100, 1293
CspAI ACCGGT 1 cut(s) 934
CviAII CATG 4 cut(s) 122, 220, 508, 568
CviQI GTAC 7 cut(s) 622, 736, 754, 794, 821, 1100, 1293
DpnI GATC 5 cut(s) 131, 580, 776, 1224, 1266
DpnII GATC 5 cut(s) 129, 578, 774, 1222, 1264
EaeI YGGCCR 2 cut(s) 1013, 1248
EagI CGGCCG 1 cut(s) 1013
Eam1104I CTCTTC 3 cut(s) 73, 531, 665
EarI CTCTTC 3 cut(s) 73, 531, 665
EclXI CGGCCG 1 cut(s) 1013
Eco130I CCWWGG 2 cut(s) 219, 973
Eco24I GRGCYC 1 cut(s) 891
Eco31I GGTCTC 1 cut(s) 787
Eco47I GGWCC 5 cut(s) 223, 354, 361, 492, 760
Eco52I CGGCCG 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 6 cut(s) 357, 470, 488, 610, 859, 884
EcoO109I RGGNCCY 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 248, 435, 948
EcoT14I CCWWGG 2 cut(s) 219, 973
EcoT38I GRGCYC 1 cut(s) 891
ErhI CCWWGG 2 cut(s) 219, 973
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 4 cut(s) 125, 223, 511, 571
FaiI YATR 6 cut(s) 123, 221, 371, 509, 569, 629
FaqI GGGAC 2 cut(s) 374, 505
FatI CATG 4 cut(s) 121, 219, 507, 567
FauI CCCGC 1 cut(s) 832
FblI GTMKAC 1 cut(s) 722
Fnu4HI GCNGC 2 cut(s) 346, 1088
FokI GGATG 3 cut(s) 302, 1225, 1252
FriOI GRGCYC 1 cut(s) 891
Fsp4HI GCNGC 2 cut(s) 346, 1088
FspBI CTAG 3 cut(s) 1236, 1283, 1297
GlaI GCGC 1 cut(s) 979
GluI GCNGC 2 cut(s) 346, 1088
GsaI CCCAGC 1 cut(s) 1166
GsuI CTGGAG 1 cut(s) 908
HaeIII GGCC 5 cut(s) 636, 690, 889, 1015, 1250
HgaI GACGC 5 cut(s) 417, 434, 551, 677, 733
HhaI GCGC 1 cut(s) 980
Hin1I GRCGYC 3 cut(s) 543, 669, 725
Hin1II CATG 4 cut(s) 125, 223, 511, 571
Hin6I GCGC 1 cut(s) 978
HinP1I GCGC 1 cut(s) 978
HincII GTYRAC 2 cut(s) 485, 723
HindII GTYRAC 2 cut(s) 485, 723
HinfI GANTC 4 cut(s) 512, 615, 641, 857
HphI GGTGA 5 cut(s) 163, 271, 1123, 1187, 1240
Hpy166II GTNNAC 7 cut(s) 242, 485, 723, 756, 856, 873, 895
Hpy188I TCNGA 5 cut(s) 457, 535, 846, 942, 1156
Hpy188III TCNNGA 4 cut(s) 610, 805, 925, 1147
Hpy8I GTNNAC 7 cut(s) 242, 485, 723, 756, 856, 873, 895
Hpy99I CGWCG 4 cut(s) 428, 671, 727, 773
HpyAV CCTTC 3 cut(s) 44, 434, 598
HpyCH4III ACNGT 6 cut(s) 56, 193, 260, 496, 735, 760
HpyCH4IV ACGT 6 cut(s) 168, 213, 285, 875, 1142, 1206
HpyCH4V TGCA 2 cut(s) 507, 627
HpyF10VI GCNNNNNNNGC 4 cut(s) 633, 677, 1021, 1030
HpySE526I ACGT 6 cut(s) 168, 213, 285, 875, 1142, 1206
Hsp92I GRCGYC 3 cut(s) 543, 669, 725
Hsp92II CATG 4 cut(s) 125, 223, 511, 571
HspAI GCGC 1 cut(s) 978
KpnI GGTACC 2 cut(s) 1103, 1296
Kzo9I GATC 5 cut(s) 129, 578, 774, 1222, 1264
LguI GCTCTTC 1 cut(s) 665
LmnI GCTCC 4 cut(s) 258, 335, 951, 1058
Lsp1109I GCAGC 1 cut(s) 1074
MaeI CTAG 3 cut(s) 1236, 1283, 1297
MaeII ACGT 6 cut(s) 168, 213, 285, 875, 1142, 1206
MaeIII GTNAC 8 cut(s) 169, 214, 592, 638, 729, 1039, 1072, 1187
MalI GATC 5 cut(s) 131, 580, 776, 1224, 1266
MboI GATC 5 cut(s) 129, 578, 774, 1222, 1264
MflI RGATCY 2 cut(s) 578, 774
MhlI GDGCHC 2 cut(s) 866, 891
MluI ACGCGT 1 cut(s) 426
MlyI GAGTC 3 cut(s) 506, 635, 851
MmeI TCCRAC 4 cut(s) 480, 490, 552, 1179
MseI TTAA 3 cut(s) 560, 695, 1053
MspA1I CMGCKG 1 cut(s) 1037
MvaI CCWGG 3 cut(s) 250, 437, 950
MvnI CGCG 5 cut(s) 428, 680, 752, 815, 980
MwoI GCNNNNNNNGC 4 cut(s) 633, 677, 1021, 1030
NcoI CCATGG 1 cut(s) 219
NdeII GATC 5 cut(s) 129, 578, 774, 1222, 1264
NlaIII CATG 4 cut(s) 125, 223, 511, 571
NmeAIII GCCGAG 2 cut(s) 666, 1276
NmuCI GTSAC 4 cut(s) 169, 638, 1039, 1187
PciSI GCTCTTC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 2 cut(s) 731, 1152
PfeI GAWTC 1 cut(s) 615
PflFI GACNNNGTC 1 cut(s) 746
PfoI TCCNGGA 2 cut(s) 785, 948
PinAI ACCGGT 1 cut(s) 934
PkrI GCNGC 2 cut(s) 347, 1089
PleI GAGTC 3 cut(s) 506, 635, 851
PpsI GAGTC 3 cut(s) 506, 635, 851
PpuMI RGGWCCY 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 248, 435, 948
PspFI CCCAGC 1 cut(s) 1162
PspGI CCWGG 3 cut(s) 248, 435, 948
PspOMI GGGCCC 1 cut(s) 887
PspPI GGNCC 9 cut(s) 223, 354, 361, 492, 634, 689, 760, 887, 888
PspPPI RGGWCCY 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 60
PsuI RGATCY 2 cut(s) 578, 774
PsyI GACNNNGTC 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 7 cut(s) 623, 737, 755, 795, 822, 1101, 1294
RsaNI GTAC 7 cut(s) 622, 736, 754, 794, 821, 1100, 1293
SalI GTCGAC 1 cut(s) 721
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 3 cut(s) 560, 695, 1053
SatI GCNGC 2 cut(s) 346, 1088
Sau3AI GATC 5 cut(s) 129, 578, 774, 1222, 1264
Sau96I GGNCC 9 cut(s) 223, 354, 361, 492, 634, 689, 760, 887, 888
ScaI AGTACT 1 cut(s) 795
SchI GAGTC 3 cut(s) 506, 635, 851
SduI GDGCHC 2 cut(s) 866, 891
SfcI CTRYAG 1 cut(s) 52
SinI GGWCC 5 cut(s) 223, 354, 361, 492, 760
SmaI CCCGGG 2 cut(s) 359, 490
SsiI CCGC 4 cut(s) 346, 813, 839, 900
SspMI CTAG 3 cut(s) 1236, 1283, 1297
StyI CCWWGG 2 cut(s) 219, 973
TaaI ACNGT 6 cut(s) 56, 193, 260, 496, 735, 760
TaiI ACGT 6 cut(s) 171, 216, 288, 878, 1145, 1209
TaqI TCGA 5 cut(s) 128, 228, 722, 741, 768
TatI WGTACW 1 cut(s) 793
TauI GCSGC 1 cut(s) 348
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 3 cut(s) 560, 695, 1053
Tru9I TTAA 3 cut(s) 560, 695, 1053
TscAI CASTG 2 cut(s) 244, 263
TseFI GTSAC 4 cut(s) 169, 638, 1039, 1187
TseI GCWGC 1 cut(s) 1087
Tsp45I GTSAC 4 cut(s) 169, 638, 1039, 1187
TspDTI ATGAA 4 cut(s) 14, 26, 584, 1022
TspMI CCCGGG 2 cut(s) 357, 488
TspRI CASTG 2 cut(s) 244, 263
Tth111I GACNNNGTC 1 cut(s) 746
VpaK11BI GGWCC 5 cut(s) 223, 354, 361, 492, 760
XmaI CCCGGG 2 cut(s) 357, 488
XmiI GTMKAC 1 cut(s) 722
XspI CTAG 3 cut(s) 1236, 1283, 1297
ZrmI AGTACT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.