Rh1CG323000

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0015032
Physical Location & Seq
Reverse (-)
59844584 .. 59847095
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG323000.1

Sequence Viewer

Length: 1080 bp
ATGGTCCTCGACACAGGCAGTGAACTCTCCTGGCTCCACTGTAAGAAAACCCCGACCCTCACCAACGTCTTCAACCCACTCGCCTCCAAATCCTACATCCCCGTCTCCTGCTCCACCCCCACTTGCCGCACCCGGACCCGGGACCTATCCATACCCGTTTCTTGCGACCCGAAAAAGCTCTGCCACGCCATTCTCTCCTACGCTGACGCCTCCTCCCTGGAAGGCAACCTCGCCTCCGACACTTTCCAACTCGGGTCGTCGGGTCAACCCGGGACCGTATTCGGGTGCATGGACTCCGGGTTCAGCTCCAACTCCGAAGAGGACGCCAAGACAACCGGGTTAATGGGCATGAACCGGGGCTCCTTATCCTTCGTAACCCAAATGGGCTTCCCGAGATTCTCGTACTGCATATCAGGCCGTGACTCCTCCGGCGTCTTGCTCTTCGGCGACGCCAACTTCGCGTGGCTCGGCCCGTTAAACTACACGCCGTTAGTCCAAATGTCGACGCCGTTACCGTACTTCGACCGGGTCGCGTACACGGTCCAGCTCGACGGGATCCGGGTCGCCGGGAAAGTACTCCCCCTCCCGAAATCCGTGTTCGTACCCGACCACACCGGGGCGGGTCAGACAATGGTGGACTCGGGCACCCAGTTCACGTTTCTTCTCGGGCCGGTTTACACCGCTTTGAAAAACGAGTTCGTTCTCCAGACCAAACCGGTTCTGAAGCTCCTGGACGACCCGAACTTCGTTTTTCAAGGCGCGATGGATTTGTGTTTTCTGGTTCCGATGAACCGGCCCGGTCTGCCGGTTCTGCCAGCTGTGACGATGATGTGTAAGGGAGCAGAGATGAGCGTAACGGGGGAAAGGCTGCTGTATCGGGTACCGGGAATGGTGAGGGGGAATAATCAGGTGTACTGCTTCACGTTCGGGAACTCCGACTTGCTGGGCGTAGAGGCGTTTGTGATTGGTCACTATCACCAGCAGAACGTGTGGATGGAGTTTGATCTGGTGAAGTCTAGGATGGGAGTGGCCGAGGTTAGGTGTGATCTAGCAAGTCAAAGGCTAGGTTTGGGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

359

Amino Acids

39.05

Weight (kDa)

6.51

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 1 - 150 3.5e-31 Xylanase inhibitor N-terminal
Asp PF00026 2 - 346 3.7e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 178 - 342 9.9e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39710
fragaria_vesca FvH4_7g22840
malus_domestica MD01G1136600.v1.1 MD07G1201600.v1.1
prunus_persica Prupe.2G238200_v2.0.a1
pyrus_communis pycom01g15730 pycom07g18860
rosa_chinensis RchiOBHm_Chr1g0368001
rosa_laevigata RLG00000027248
rosa_roxburghii Rroxscaffold_4G00288960
rosa_rugosa Rorug01G0338400
rosa_samantha Rh1AG345100 Rh1BG307400 Rh1CG323000 Rh1DG339500
rosa_wichuraiana Rw1G030710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 880, 1073
AccB1I GGYRCC 3 cut(s) 644, 880, 1073
AccI GTMKAC 1 cut(s) 503
AccII CGCG 3 cut(s) 461, 533, 761
AciI CCGC 3 cut(s) 127, 620, 681
AclWI GGATC 2 cut(s) 550, 563
AcoI YGGCCR 1 cut(s) 1029
AcuI CTGAAG 1 cut(s) 743
AcyI GRCGYC 5 cut(s) 207, 324, 432, 450, 506
AfaI GTAC 8 cut(s) 404, 518, 536, 576, 603, 882, 914, 1075
AfiI CCNNNNNNNGG 5 cut(s) 138, 139, 282, 616, 1038
AflIII ACRYGT 1 cut(s) 987
AgeI ACCGGT 1 cut(s) 715
AgsI TTSAA 3 cut(s) 73, 688, 755
AjnI CCWGG 3 cut(s) 29, 216, 729
AloI GAACNNNNNNTCC 4 cut(s) 284, 316, 344, 376
AluBI AGCT 5 cut(s) 178, 306, 547, 727, 818
AluI AGCT 5 cut(s) 178, 306, 547, 727, 818
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 2 cut(s) 550, 563
Ama87I CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
AoxI GGCC 5 cut(s) 415, 469, 668, 794, 1029
ApeKI GCWGC 1 cut(s) 868
ArsI GACNNNNNNTTYG 4 cut(s) 440, 472, 728, 760
AsiGI ACCGGT 1 cut(s) 715
Asp718I GGTACC 2 cut(s) 880, 1073
AspLEI GCGC 1 cut(s) 761
AspS9I GGNCC 8 cut(s) 4, 135, 142, 273, 470, 541, 668, 795
AsuHPI GGTGA 4 cut(s) 52, 904, 968, 1021
AvaI CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
AvaII GGWCC 5 cut(s) 4, 135, 142, 273, 541
BaeGI GKGCMC 1 cut(s) 647
BamHI GGATCC 1 cut(s) 555
BanI GGYRCC 3 cut(s) 644, 880, 1073
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 61
BbvI GCAGC 1 cut(s) 855
BccI CCATC 3 cut(s) 757, 988, 1015
BceAI ACGGC 3 cut(s) 402, 472, 493
BciT130I CCWGG 3 cut(s) 31, 218, 731
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 4 cut(s) 1017, 1049, 1064, 1078
BisI GCNGC 2 cut(s) 127, 869
BlsI GCNGC 2 cut(s) 128, 870
BmcAI AGTACT 1 cut(s) 576
Bme18I GGWCC 5 cut(s) 4, 135, 142, 273, 541
BmeT110I CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
BmgT120I GGNCC 8 cut(s) 4, 135, 142, 273, 470, 541, 668, 795
BmrI ACTGGG 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 643
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 689
BsaHI GRCGYC 5 cut(s) 207, 324, 432, 450, 506
BsaJI CCNNGG 6 cut(s) 138, 216, 269, 355, 615, 1032
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 8 cut(s) 197, 218, 227, 248, 344, 374, 567, 597
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 139, 282, 616, 1038
Bse118I RCCGGY 4 cut(s) 670, 715, 792, 805
Bse1I ACTGG 1 cut(s) 649
BseBI CCWGG 3 cut(s) 31, 218, 731
BseDI CCNNGG 6 cut(s) 138, 216, 269, 355, 615, 1032
BseGI GGATG 3 cut(s) 96, 999, 1026
BseLI CCNNNNNNNGG 5 cut(s) 138, 139, 282, 616, 1038
BseNI ACTGG 1 cut(s) 649
BseRI GAGGAG 2 cut(s) 202, 415
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 1 cut(s) 855
BseYI CCCAGC 1 cut(s) 943
Bsh1236I CGCG 3 cut(s) 461, 533, 761
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 5 cut(s) 417, 471, 670, 796, 1031
BshNI GGYRCC 3 cut(s) 644, 880, 1073
BshTI ACCGGT 1 cut(s) 715
BsiEI CGRYCG 1 cut(s) 526
BsiHKCI CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
BslFI GGGAC 2 cut(s) 155, 286
BslI CCNNNNNNNGG 5 cut(s) 138, 139, 282, 616, 1038
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 155, 286
BsnI GGCC 5 cut(s) 417, 471, 670, 796, 1031
BsoBI CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
Bsp1286I GDGCHC 2 cut(s) 362, 647
Bsp143I GATC 3 cut(s) 555, 1003, 1045
BspACI CCGC 3 cut(s) 127, 620, 681
BspANI GGCC 5 cut(s) 417, 471, 670, 796, 1031
BspFNI CGCG 3 cut(s) 461, 533, 761
BspPI GGATC 2 cut(s) 550, 563
BspQI GCTCTTC 1 cut(s) 446
BspT107I GGYRCC 3 cut(s) 644, 880, 1073
BsrFI RCCGGY 4 cut(s) 670, 715, 792, 805
BsrI ACTGG 1 cut(s) 649
BssAI RCCGGY 4 cut(s) 670, 715, 792, 805
BssECI CCNNGG 6 cut(s) 138, 216, 269, 355, 615, 1032
BssMI GATC 3 cut(s) 555, 1003, 1045
BssNI GRCGYC 5 cut(s) 207, 324, 432, 450, 506
Bst2UI CCWGG 3 cut(s) 31, 218, 731
Bst4CI ACNGT 4 cut(s) 41, 277, 516, 541
Bst6I CTCTTC 2 cut(s) 312, 446
BstACI GRCGYC 5 cut(s) 207, 324, 432, 450, 506
BstC8I GCNNGC 1 cut(s) 816
BstF5I GGATG 3 cut(s) 96, 999, 1026
BstFNI CGCG 3 cut(s) 461, 533, 761
BstHHI GCGC 1 cut(s) 761
BstKTI GATC 3 cut(s) 558, 1006, 1048
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 555, 1003, 1045
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 4 cut(s) 414, 458, 802, 811
BstNI CCWGG 3 cut(s) 31, 218, 731
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 3 cut(s) 461, 533, 761
BstV1I GCAGC 1 cut(s) 855
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 555
BstYI RGATCY 1 cut(s) 555
BsuRI GGCC 5 cut(s) 417, 471, 670, 796, 1031
BtgZI GCGATG 1 cut(s) 776
BtsCI GGATG 3 cut(s) 96, 999, 1026
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 37
Cac8I GCNNGC 1 cut(s) 816
CfoI GCGC 1 cut(s) 761
Cfr10I RCCGGY 4 cut(s) 670, 715, 792, 805
Cfr13I GGNCC 8 cut(s) 4, 135, 142, 273, 470, 541, 668, 795
Cfr9I CCCGGG 2 cut(s) 138, 269
CseI GACGC 5 cut(s) 215, 332, 421, 458, 514
Csp6I GTAC 8 cut(s) 403, 517, 535, 575, 602, 881, 913, 1074
CspAI ACCGGT 1 cut(s) 715
CviAII CATG 2 cut(s) 289, 349
CviQI GTAC 8 cut(s) 403, 517, 535, 575, 602, 881, 913, 1074
DpnI GATC 3 cut(s) 557, 1005, 1047
DpnII GATC 3 cut(s) 555, 1003, 1045
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 312, 446
EarI CTCTTC 2 cut(s) 312, 446
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 5 cut(s) 4, 135, 142, 273, 541
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 6 cut(s) 138, 251, 269, 391, 640, 665
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 3 cut(s) 29, 216, 729
EcoT38I GRGCYC 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 2 cut(s) 292, 352
FaiI YATR 4 cut(s) 152, 290, 350, 410
FaqI GGGAC 2 cut(s) 155, 286
FatI CATG 2 cut(s) 288, 348
FauI CCCGC 1 cut(s) 613
FblI GTMKAC 1 cut(s) 503
Fnu4HI GCNGC 2 cut(s) 127, 869
FokI GGATG 3 cut(s) 83, 1006, 1033
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 2 cut(s) 127, 869
FspBI CTAG 4 cut(s) 1017, 1049, 1064, 1078
GlaI GCGC 1 cut(s) 760
GluI GCNGC 2 cut(s) 127, 869
GsaI CCCAGC 1 cut(s) 947
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 5 cut(s) 417, 471, 670, 796, 1031
HgaI GACGC 5 cut(s) 215, 332, 421, 458, 514
HhaI GCGC 1 cut(s) 761
Hin1I GRCGYC 5 cut(s) 207, 324, 432, 450, 506
Hin1II CATG 2 cut(s) 292, 352
Hin6I GCGC 1 cut(s) 759
HinP1I GCGC 1 cut(s) 759
HincII GTYRAC 2 cut(s) 266, 504
HindII GTYRAC 2 cut(s) 266, 504
HinfI GANTC 4 cut(s) 293, 396, 422, 638
HphI GGTGA 4 cut(s) 52, 904, 968, 1021
Hpy166II GTNNAC 8 cut(s) 23, 266, 504, 537, 637, 654, 676, 913
Hpy188I TCNGA 6 cut(s) 238, 316, 627, 723, 786, 937
Hpy188III TCNNGA 4 cut(s) 391, 586, 706, 928
Hpy8I GTNNAC 8 cut(s) 23, 266, 504, 537, 637, 654, 676, 913
Hpy99I CGWCG 4 cut(s) 262, 452, 508, 554
HpyAV CCTTC 2 cut(s) 215, 379
HpyCH4III ACNGT 4 cut(s) 41, 277, 516, 541
HpyCH4IV ACGT 4 cut(s) 66, 656, 923, 987
HpyCH4V TGCA 2 cut(s) 288, 408
HpyF10VI GCNNNNNNNGC 4 cut(s) 414, 458, 802, 811
HpySE526I ACGT 4 cut(s) 66, 656, 923, 987
Hsp92I GRCGYC 5 cut(s) 207, 324, 432, 450, 506
Hsp92II CATG 2 cut(s) 292, 352
HspAI GCGC 1 cut(s) 759
KpnI GGTACC 2 cut(s) 884, 1077
Kzo9I GATC 3 cut(s) 555, 1003, 1045
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 6 cut(s) 39, 116, 311, 365, 732, 839
Lsp1109I GCAGC 1 cut(s) 855
MaeI CTAG 4 cut(s) 1017, 1049, 1064, 1078
MaeII ACGT 4 cut(s) 66, 656, 923, 987
MaeIII GTNAC 6 cut(s) 373, 419, 510, 820, 853, 968
MalI GATC 3 cut(s) 557, 1005, 1047
MboI GATC 3 cut(s) 555, 1003, 1045
MboII GAAGA 4 cut(s) 61, 329, 433, 653
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 2 cut(s) 362, 647
MlyI GAGTC 3 cut(s) 287, 416, 632
MmeI TCCRAC 4 cut(s) 261, 271, 333, 960
MseI TTAA 2 cut(s) 341, 476
MspA1I CMGCKG 1 cut(s) 818
MvaI CCWGG 3 cut(s) 31, 218, 731
MvnI CGCG 3 cut(s) 461, 533, 761
MwoI GCNNNNNNNGC 4 cut(s) 414, 458, 802, 811
NdeII GATC 3 cut(s) 555, 1003, 1045
NlaIII CATG 2 cut(s) 292, 352
NmeAIII GCCGAG 2 cut(s) 447, 1057
NmuCI GTSAC 3 cut(s) 419, 820, 968
PciSI GCTCTTC 1 cut(s) 446
PcsI WCGNNNNNNNCGW 2 cut(s) 512, 933
PfeI GAWTC 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 729
PinAI ACCGGT 1 cut(s) 715
PkrI GCNGC 2 cut(s) 128, 870
PleI GAGTC 3 cut(s) 287, 416, 632
PpsI GAGTC 3 cut(s) 287, 416, 632
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 3 cut(s) 29, 216, 729
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 3 cut(s) 29, 216, 729
PspPI GGNCC 8 cut(s) 4, 135, 142, 273, 470, 541, 668, 795
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 1 cut(s) 555
PsyI GACNNNGTC 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 8 cut(s) 404, 518, 536, 576, 603, 882, 914, 1075
RsaNI GTAC 8 cut(s) 403, 517, 535, 575, 602, 881, 913, 1074
SalI GTCGAC 1 cut(s) 502
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 2 cut(s) 341, 476
SatI GCNGC 2 cut(s) 127, 869
Sau3AI GATC 3 cut(s) 555, 1003, 1045
Sau96I GGNCC 8 cut(s) 4, 135, 142, 273, 470, 541, 668, 795
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 3 cut(s) 287, 416, 632
SduI GDGCHC 2 cut(s) 362, 647
SinI GGWCC 5 cut(s) 4, 135, 142, 273, 541
SmaI CCCGGG 2 cut(s) 140, 271
SsiI CCGC 3 cut(s) 127, 620, 681
SspMI CTAG 4 cut(s) 1017, 1049, 1064, 1078
TaaI ACNGT 4 cut(s) 41, 277, 516, 541
TaiI ACGT 4 cut(s) 69, 659, 926, 990
TaqI TCGA 4 cut(s) 9, 503, 522, 549
TatI WGTACW 2 cut(s) 574, 912
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 2 cut(s) 341, 476
Tru9I TTAA 2 cut(s) 341, 476
TscAI CASTG 2 cut(s) 25, 44
TseFI GTSAC 3 cut(s) 419, 820, 968
TseI GCWGC 1 cut(s) 868
Tsp45I GTSAC 3 cut(s) 419, 820, 968
TspDTI ATGAA 2 cut(s) 365, 803
TspGWI ACGGA 1 cut(s) 583
TspMI CCCGGG 2 cut(s) 138, 269
TspRI CASTG 2 cut(s) 25, 44
Tth111I GACNNNGTC 1 cut(s) 527
VpaK11BI GGWCC 5 cut(s) 4, 135, 142, 273, 541
XmaI CCCGGG 2 cut(s) 138, 269
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 4 cut(s) 1017, 1049, 1064, 1078
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.