RLG00000027248

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015032
Physical Location & Seq
Forward (+)
9957762 .. 9960191
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027248

Sequence Viewer

Length: 1293 bp
ATGCCACTTCTTCTTCATCATCTTCTCCTTCATTTTTTTACTTACTACAGTCTCTGCTTCTCTTCTCCACTACCTAAAACCCTCATTCTACCTCTCAAAACCCAGACACTCCCACATGGGTCGATCCCAAAACCCTCAAACAAGCTCTCATTCCACCACAACGTCACCCTAACCATCTCCCTCACAGTCGGGTCGCCCCCACAGAACGTTACCATGGTCCTCGACACAGGCAGTGAGCTCTCCTGGCTCCACTGTAAGAAAACCCCGACCCTCACCAACGTCTTTAACCCACTCGCCTCCAAATCCTACATCCCCGTCTCCTGCTCCACCCCCACTTGCCGCACCCAGACCCGGGACCTATCCATACCCGTTTCTTGCGACCCGAAAAAGCTCTGCCACGCCATTCTCTCCTACGCTGACGCCTCCTCCCTGGAAGGCAACCTCGCCTCCGACACTTTCCAACTCGGGTCGTCGGGTCAACCCGGGACCGTATTCGGGTGCATGGACTCCGGGTTCAGCTCCAACTCCGAAGAGGACGCCAAGACAACCGGCTTAATGGGCATGAACCGGGGCTCCTTATCCTTCGTGACCCAAATGGGCTTCCCGAGATTCTCGTACTGCATATCGGGCCGTGACTCCTCCGGCGTCTTGCTCTTCGGCGACGCCAACTTTGCGTGGCTCGGGCCGTTAAACTACACGCCGTTAGTCCAAATGTCGACGCCGTTACCGTACTTCGACCGGGTTGCGTACACGGTCCAGCTCGACGGGATCCGGGTCGCCGGGAAAGTACTCCCCCTCCCGAAATCCGTGTTCGTACCAGACCACACCGGGGCGGGTCAGACAATGGTGGACTCGGGCACCCAGTTCACGTTTCTTCTCGGGCCGGTTTACACCGCTCTGAAAAACGAGTTCGTTCTCCAGACCAAACCGGTTCTGAAGCTCCTGGACGACCCGAACTTCGTTTTTCAAGGCGCGATGGATTTGTGTTTTCTGGTTCCCATGAACCGGCCCGGTCTGCCGGTTCTGCCAGCTGTGACGATGATGTTTAAGGGAGCAGAGATGAGCGTAACGGGGGAAAGGCTGCTGTATCGGGTACCGGGAATGGTGAGGGGGAATAATCAGGTGTATTGCTTCACGTTCGGGAACTCCGACTTGCTGGGCGTAGAAGCGTTTGTGATTGGTCACTATCACCAGCAGAACGTGTGGATGGAGTTTGATCTGGTGAAGTCTAGGATGGGACTGGCCGAGGTTAGGTGTGATCTCGCAAGTCAAAGGCTAGGTTTGGGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

431

Amino Acids

46.99

Weight (kDa)

7.97

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 59 - 221 2.3e-36 Xylanase inhibitor N-terminal
Asp PF00026 60 - 417 1.9e-14 Eukaryotic aspartyl protease
TAXi_C PF14541 249 - 414 5.3e-31 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39710
fragaria_vesca FvH4_7g22840
malus_domestica MD01G1136600.v1.1 MD07G1201600.v1.1
prunus_persica Prupe.2G238200_v2.0.a1
pyrus_communis pycom01g15730 pycom07g18860
rosa_chinensis RchiOBHm_Chr1g0368001
rosa_laevigata RLG00000027248
rosa_roxburghii Rroxscaffold_4G00288960
rosa_rugosa Rorug01G0338400
rosa_samantha Rh1AG345100 Rh1BG307400 Rh1CG323000 Rh1DG339500
rosa_wichuraiana Rw1G030710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1093, 1286
AccB1I GGYRCC 3 cut(s) 857, 1093, 1286
AccBSI CCGCTC 1 cut(s) 896
AccI GTMKAC 1 cut(s) 716
AccII CGCG 1 cut(s) 974
AciI CCGC 3 cut(s) 340, 833, 894
AclI AACGTT 1 cut(s) 207
AclWI GGATC 3 cut(s) 118, 763, 776
AcoI YGGCCR 1 cut(s) 1242
AcuI CTGAAG 1 cut(s) 956
AcyI GRCGYC 5 cut(s) 420, 537, 645, 663, 719
AfaI GTAC 7 cut(s) 617, 731, 749, 789, 816, 1095, 1288
AfiI CCNNNNNNNGG 6 cut(s) 351, 352, 495, 829, 1005, 1251
AflIII ACRYGT 1 cut(s) 1200
AgeI ACCGGT 1 cut(s) 928
AgsI TTSAA 1 cut(s) 968
AjnI CCWGG 3 cut(s) 242, 429, 942
AloI GAACNNNNNNTCC 4 cut(s) 497, 529, 557, 589
AluBI AGCT 7 cut(s) 145, 238, 391, 519, 760, 940, 1031
AluI AGCT 7 cut(s) 145, 238, 391, 519, 760, 940, 1031
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 56, 322
AlwI GGATC 3 cut(s) 118, 763, 776
AlwNI CAGNNNCTG 1 cut(s) 54
Ama87I CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
AoxI GGCC 5 cut(s) 628, 683, 881, 1007, 1242
ApeKI GCWGC 1 cut(s) 1081
ArsI GACNNNNNNTTYG 4 cut(s) 653, 685, 941, 973
AsiGI ACCGGT 1 cut(s) 928
Asp718I GGTACC 2 cut(s) 1093, 1286
AspLEI GCGC 1 cut(s) 974
AspS9I GGNCC 8 cut(s) 217, 355, 486, 628, 683, 754, 881, 1008
AsuHPI GGTGA 5 cut(s) 157, 265, 1117, 1181, 1234
AvaI CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
AvaII GGWCC 4 cut(s) 217, 355, 486, 754
BaeGI GKGCMC 1 cut(s) 860
BamHI GGATCC 1 cut(s) 768
BanI GGYRCC 3 cut(s) 857, 1093, 1286
BanII GRGCYC 2 cut(s) 240, 575
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 1068
BccI CCATC 4 cut(s) 182, 970, 1201, 1228
BceAI ACGGC 4 cut(s) 615, 670, 685, 706
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BciT130I CCWGG 3 cut(s) 244, 431, 944
BcoDI GTCTC 2 cut(s) 56, 322
BfaI CTAG 3 cut(s) 1230, 1277, 1291
BfmI CTRYAG 1 cut(s) 46
BisI GCNGC 2 cut(s) 340, 1082
BlsI GCNGC 2 cut(s) 341, 1083
BmcAI AGTACT 1 cut(s) 789
Bme18I GGWCC 4 cut(s) 217, 355, 486, 754
BmeT110I CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
BmgT120I GGNCC 8 cut(s) 217, 355, 486, 628, 683, 754, 881, 1008
BmiI GGNNCC 9 cut(s) 248, 356, 487, 574, 770, 859, 996, 1095, 1288
BmrI ACTGGG 1 cut(s) 856
BmuI ACTGGG 1 cut(s) 856
BpmI CTGGAG 1 cut(s) 902
BsaHI GRCGYC 5 cut(s) 420, 537, 645, 663, 719
BsaJI CCNNGG 7 cut(s) 213, 351, 429, 482, 568, 828, 1245
BsaWI WCCGGW 1 cut(s) 928
BsaXI ACNNNNNCTCC 8 cut(s) 410, 431, 440, 461, 557, 587, 780, 810
Bsc4I CCNNNNNNNGG 6 cut(s) 351, 352, 495, 829, 1005, 1251
Bse118I RCCGGY 5 cut(s) 548, 883, 928, 1005, 1018
Bse1I ACTGG 2 cut(s) 862, 1245
BseBI CCWGG 3 cut(s) 244, 431, 944
BseDI CCNNGG 7 cut(s) 213, 351, 429, 482, 568, 828, 1245
BseGI GGATG 3 cut(s) 309, 1212, 1239
BseLI CCNNNNNNNGG 6 cut(s) 351, 352, 495, 829, 1005, 1251
BseNI ACTGG 2 cut(s) 862, 1245
BseRI GAGGAG 2 cut(s) 415, 628
BseSI GKGCMC 1 cut(s) 860
BseXI GCAGC 1 cut(s) 1068
BseYI CCCAGC 1 cut(s) 1156
Bsh1236I CGCG 1 cut(s) 974
Bsh1285I CGRYCG 1 cut(s) 739
BshFI GGCC 5 cut(s) 630, 685, 883, 1009, 1244
BshNI GGYRCC 3 cut(s) 857, 1093, 1286
BshTI ACCGGT 1 cut(s) 928
BsiEI CGRYCG 1 cut(s) 739
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
BslFI GGGAC 3 cut(s) 368, 499, 1251
BslI CCNNNNNNNGG 6 cut(s) 351, 352, 495, 829, 1005, 1251
BsmAI GTCTC 2 cut(s) 56, 322
BsmBI CGTCTC 1 cut(s) 322
BsmFI GGGAC 3 cut(s) 368, 499, 1251
BsnI GGCC 5 cut(s) 630, 685, 883, 1009, 1244
BsoBI CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
Bsp1286I GDGCHC 3 cut(s) 240, 575, 860
Bsp143I GATC 4 cut(s) 123, 768, 1216, 1258
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 3 cut(s) 340, 833, 894
BspANI GGCC 5 cut(s) 630, 685, 883, 1009, 1244
BspFNI CGCG 1 cut(s) 974
BspLI GGNNCC 9 cut(s) 248, 356, 487, 574, 770, 859, 996, 1095, 1288
BspPI GGATC 3 cut(s) 118, 763, 776
BspQI GCTCTTC 1 cut(s) 659
BspT107I GGYRCC 3 cut(s) 857, 1093, 1286
BsrBI CCGCTC 1 cut(s) 896
BsrFI RCCGGY 5 cut(s) 548, 883, 928, 1005, 1018
BsrI ACTGG 2 cut(s) 862, 1245
BssAI RCCGGY 5 cut(s) 548, 883, 928, 1005, 1018
BssECI CCNNGG 7 cut(s) 213, 351, 429, 482, 568, 828, 1245
BssMI GATC 4 cut(s) 123, 768, 1216, 1258
BssNI GRCGYC 5 cut(s) 420, 537, 645, 663, 719
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 3 cut(s) 244, 431, 944
Bst4CI ACNGT 6 cut(s) 50, 187, 254, 490, 729, 754
Bst6I CTCTTC 3 cut(s) 67, 525, 659
BstACI GRCGYC 5 cut(s) 420, 537, 645, 663, 719
BstC8I GCNNGC 1 cut(s) 1029
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 3 cut(s) 309, 1212, 1239
BstFNI CGCG 1 cut(s) 974
BstHHI GCGC 1 cut(s) 974
BstKTI GATC 4 cut(s) 126, 771, 1219, 1261
BstMAI GTCTC 2 cut(s) 56, 322
BstMBI GATC 4 cut(s) 123, 768, 1216, 1258
BstMCI CGRYCG 1 cut(s) 739
BstMWI GCNNNNNNNGC 6 cut(s) 244, 558, 627, 671, 1015, 1024
BstNI CCWGG 3 cut(s) 244, 431, 944
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 860
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 1 cut(s) 1068
BstX2I RGATCY 1 cut(s) 768
BstYI RGATCY 1 cut(s) 768
BsuRI GGCC 5 cut(s) 630, 685, 883, 1009, 1244
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 989
BtsCI GGATG 3 cut(s) 309, 1212, 1239
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 250
Cac8I GCNNGC 1 cut(s) 1029
CaiI CAGNNNCTG 1 cut(s) 54
CfoI GCGC 1 cut(s) 974
Cfr10I RCCGGY 5 cut(s) 548, 883, 928, 1005, 1018
Cfr13I GGNCC 8 cut(s) 217, 355, 486, 628, 683, 754, 881, 1008
Cfr9I CCCGGG 2 cut(s) 351, 482
CseI GACGC 5 cut(s) 428, 545, 634, 671, 727
Csp6I GTAC 7 cut(s) 616, 730, 748, 788, 815, 1094, 1287
CspAI ACCGGT 1 cut(s) 928
CviAII CATG 5 cut(s) 116, 214, 502, 562, 1000
CviQI GTAC 7 cut(s) 616, 730, 748, 788, 815, 1094, 1287
DpnI GATC 4 cut(s) 125, 770, 1218, 1260
DpnII GATC 4 cut(s) 123, 768, 1216, 1258
EaeI YGGCCR 1 cut(s) 1242
Eam1104I CTCTTC 3 cut(s) 67, 525, 659
EarI CTCTTC 3 cut(s) 67, 525, 659
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 2 cut(s) 240, 575
Eco47I GGWCC 4 cut(s) 217, 355, 486, 754
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 956
Eco88I CYCGRG 7 cut(s) 351, 464, 482, 604, 680, 853, 878
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 242, 429, 942
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 2 cut(s) 240, 575
ErhI CCWWGG 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 5 cut(s) 119, 217, 505, 565, 1003
FaiI YATR 7 cut(s) 117, 215, 365, 503, 563, 623, 1001
FaqI GGGAC 3 cut(s) 368, 499, 1251
FatI CATG 5 cut(s) 115, 213, 501, 561, 999
FauI CCCGC 1 cut(s) 826
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 2 cut(s) 340, 1082
FokI GGATG 3 cut(s) 296, 1219, 1246
FriOI GRGCYC 2 cut(s) 240, 575
Fsp4HI GCNGC 2 cut(s) 340, 1082
FspBI CTAG 3 cut(s) 1230, 1277, 1291
GlaI GCGC 1 cut(s) 973
GluI GCNGC 2 cut(s) 340, 1082
GsaI CCCAGC 1 cut(s) 1160
GsuI CTGGAG 1 cut(s) 902
HaeIII GGCC 5 cut(s) 630, 685, 883, 1009, 1244
HgaI GACGC 5 cut(s) 428, 545, 634, 671, 727
HhaI GCGC 1 cut(s) 974
Hin1I GRCGYC 5 cut(s) 420, 537, 645, 663, 719
Hin1II CATG 5 cut(s) 119, 217, 505, 565, 1003
Hin6I GCGC 1 cut(s) 972
HinP1I GCGC 1 cut(s) 972
HincII GTYRAC 2 cut(s) 479, 717
HindII GTYRAC 2 cut(s) 479, 717
HinfI GANTC 4 cut(s) 506, 609, 635, 851
HphI GGTGA 5 cut(s) 157, 265, 1117, 1181, 1234
Hpy166II GTNNAC 6 cut(s) 479, 717, 750, 850, 867, 889
Hpy188I TCNGA 6 cut(s) 451, 529, 840, 900, 936, 1150
Hpy188III TCNNGA 5 cut(s) 586, 604, 799, 919, 1141
Hpy8I GTNNAC 6 cut(s) 479, 717, 750, 850, 867, 889
Hpy99I CGWCG 4 cut(s) 475, 665, 721, 767
HpyAV CCTTC 3 cut(s) 38, 428, 592
HpyCH4III ACNGT 6 cut(s) 50, 187, 254, 490, 729, 754
HpyCH4IV ACGT 6 cut(s) 162, 207, 279, 869, 1136, 1200
HpyCH4V TGCA 2 cut(s) 501, 621
HpyF10VI GCNNNNNNNGC 6 cut(s) 244, 558, 627, 671, 1015, 1024
HpySE526I ACGT 6 cut(s) 162, 207, 279, 869, 1136, 1200
Hsp92I GRCGYC 5 cut(s) 420, 537, 645, 663, 719
Hsp92II CATG 5 cut(s) 119, 217, 505, 565, 1003
HspAI GCGC 1 cut(s) 972
KpnI GGTACC 2 cut(s) 1097, 1290
Kzo9I GATC 4 cut(s) 123, 768, 1216, 1258
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 6 cut(s) 252, 329, 524, 578, 945, 1052
Lsp1109I GCAGC 1 cut(s) 1068
MaeI CTAG 3 cut(s) 1230, 1277, 1291
MaeII ACGT 6 cut(s) 162, 207, 279, 869, 1136, 1200
MaeIII GTNAC 8 cut(s) 163, 208, 586, 632, 723, 1033, 1066, 1181
MalI GATC 4 cut(s) 125, 770, 1218, 1260
MbiI CCGCTC 1 cut(s) 896
MboI GATC 4 cut(s) 123, 768, 1216, 1258
MboII GAAGA 6 cut(s) 5, 14, 54, 542, 646, 866
MflI RGATCY 1 cut(s) 768
MhlI GDGCHC 3 cut(s) 240, 575, 860
MlyI GAGTC 3 cut(s) 500, 629, 845
MmeI TCCRAC 4 cut(s) 474, 484, 546, 1173
MseI TTAA 4 cut(s) 285, 554, 689, 1047
MspA1I CMGCKG 1 cut(s) 1031
MvaI CCWGG 3 cut(s) 244, 431, 944
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 6 cut(s) 244, 558, 627, 671, 1015, 1024
NcoI CCATGG 1 cut(s) 213
NdeII GATC 4 cut(s) 123, 768, 1216, 1258
NlaIII CATG 5 cut(s) 119, 217, 505, 565, 1003
NlaIV GGNNCC 9 cut(s) 248, 356, 487, 574, 770, 859, 996, 1095, 1288
NmeAIII GCCGAG 1 cut(s) 1270
NmuCI GTSAC 5 cut(s) 163, 586, 632, 1033, 1181
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 2 cut(s) 725, 1146
PfeI GAWTC 1 cut(s) 609
PfoI TCCNGGA 1 cut(s) 942
PinAI ACCGGT 1 cut(s) 928
PkrI GCNGC 2 cut(s) 341, 1083
PleI GAGTC 3 cut(s) 500, 629, 845
PpsI GAGTC 3 cut(s) 500, 629, 845
PpuMI RGGWCCY 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 3 cut(s) 242, 429, 942
PspFI CCCAGC 1 cut(s) 1156
PspGI CCWGG 3 cut(s) 242, 429, 942
PspN4I GGNNCC 9 cut(s) 248, 356, 487, 574, 770, 859, 996, 1095, 1288
PspPI GGNCC 8 cut(s) 217, 355, 486, 628, 683, 754, 881, 1008
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 54
PsuI RGATCY 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 7 cut(s) 617, 731, 749, 789, 816, 1095, 1288
RsaNI GTAC 7 cut(s) 616, 730, 748, 788, 815, 1094, 1287
SacI GAGCTC 1 cut(s) 240
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 4 cut(s) 285, 554, 689, 1047
SatI GCNGC 2 cut(s) 340, 1082
Sau3AI GATC 4 cut(s) 123, 768, 1216, 1258
Sau96I GGNCC 8 cut(s) 217, 355, 486, 628, 683, 754, 881, 1008
ScaI AGTACT 1 cut(s) 789
SchI GAGTC 3 cut(s) 500, 629, 845
SduI GDGCHC 3 cut(s) 240, 575, 860
SfcI CTRYAG 1 cut(s) 46
SinI GGWCC 4 cut(s) 217, 355, 486, 754
SmaI CCCGGG 2 cut(s) 353, 484
SsiI CCGC 3 cut(s) 340, 833, 894
SspMI CTAG 3 cut(s) 1230, 1277, 1291
SstI GAGCTC 1 cut(s) 240
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 6 cut(s) 50, 187, 254, 490, 729, 754
TaiI ACGT 6 cut(s) 165, 210, 282, 872, 1139, 1203
TaqI TCGA 5 cut(s) 122, 222, 716, 735, 762
TatI WGTACW 1 cut(s) 787
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 609
Tru1I TTAA 4 cut(s) 285, 554, 689, 1047
Tru9I TTAA 4 cut(s) 285, 554, 689, 1047
TscAI CASTG 2 cut(s) 238, 257
TseFI GTSAC 5 cut(s) 163, 586, 632, 1033, 1181
TseI GCWGC 1 cut(s) 1081
Tsp45I GTSAC 5 cut(s) 163, 586, 632, 1033, 1181
TspDTI ATGAA 4 cut(s) 5, 20, 578, 1016
TspGWI ACGGA 1 cut(s) 796
TspMI CCCGGG 2 cut(s) 351, 482
TspRI CASTG 2 cut(s) 238, 257
VpaK11BI GGWCC 4 cut(s) 217, 355, 486, 754
XmaI CCCGGG 2 cut(s) 351, 482
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 3 cut(s) 1230, 1277, 1291
ZrmI AGTACT 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.