Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003169

Physical Location & Seq

Genomic Coordinates Forward (+)

25588422 .. 25595993

Gene Structure 7572 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1146300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGGAGGCCACCATAGCTTGCTGCTCATCCGCCAGGGCGCTCCTGCCGGCTGCCGGCATTTCGACTCACCAGCATTCCACTTCAGTTGTGGCTCCATCTTTCAGCTCCAAGGGCCTAAAATCAAGCTCCCTCTTCGGAGAATCACTACGACAAGTGCCACGATCATCGCTCAAGTTTTCTACAACAAAACAGTCCTCCCATGTGATCAAGTGTGAGATCGGCGATAGCCTGGAGGAGTTTCTTGCAAAGGCAACCCCGGATAAGGGACTGATCAGGTTGCTGTTAAGCATGGGAGAAGCATTGAGGACTATATCCTTCAAGGTCAGAACAGCTTCTTGTGGAGGAACAGCCTGCGTTAATTCTTTCGGAGATGAGCAGCTCGCCGTGGATATGCTTGCTGACAAGCTTTTGTTCGAGGCCTTAAGTTACTCGCACGTCTGCAAATACGCTTGCTCTGAAGAGGTTCCGGAACTCCAAGACATGGGAGGCCCAGTTGAAGGTGGATTTAGTGTTGCTTTTGATCCGCTTGATGGCTCCAGCATAGTCGACACAAACTTCACAGTAGGGACAATCTTTGGTGTGTGGCCAGGGGATAAGTTAACTGGGGTAACAGGAAGAGACCAAGTTGCTGCAGCCATGGGGATTTATGGTCCTAGAACTACATATGTTCTTGCTATCAAGGGCTTCCCCGGAACCCACGAGTTTCTTCTTCTTGATGAAGGAAAGTGGCAACATGTCAAAGAGACAACAGAGATTGGTGAAGGAAAAATGTTTTCGCCCGGGAATTTGAGAGCCACATTTGACAATCCAGACTATGACAAGTTGATCAGCTACTACGTAAAAGAGAAGTACACGTTGAGATACACCGGAGGAATGGTGCCAGATGTCAATCAGATAATTGTGAAAGAGAAGGGGGTTTTCACAAACGTGATATCTCCAACTACGAAAGCCAAGCTGAGGCTGTTATTTGAGGTAGCTCCATTAGGGTTGTTGATTGAGAATGCTGGAGGTTATAGTAGTGATGGTCAGCAGTCTGTGCTAGACAAGGTCGTTGTTAACCTTGACGATAGAACTCAGGTTGCTTATGGATCAAAAAATGAGATAATCCGATTCGAAGAAACTTTATACGGATCATCCAGGCTCAAAGGTGTGCCTGTTGGAGCTGCAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0005986 GO:0005996 GO:0006000 GO:0006002 GO:0006006 GO:0006073 GO:0006094 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009311 GO:0009312 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009941 GO:0009987 GO:0015977 GO:0015979 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0019252 GO:0019253 GO:0019318 GO:0019319 GO:0019637 GO:0019685 GO:0030388 GO:0031967 GO:0031975 GO:0033692 GO:0034637 GO:0034645 GO:0042132 GO:0042578 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046351 GO:0046364 GO:0048046 GO:0050278 GO:0050308 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0098542 GO:1901135 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00710 ko01100 ko01120 ko01200 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

FBPase

Protein Analysis

391

Amino Acids

41.93

Weight (kDa)

5.79

Isoelectric Point (pI)

33.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FBPase	PF00316	69 - 248	6.9e-57	Fructose-1-6-bisphosphatase, N-terminal domain
FBPase_C	PF18913	254 - 374	3.7e-37	Fructose-1-6-bisphosphatase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55800
fragaria_vesca	FvH4_3g15380 FvH4_7g23960
malus_domestica	MD01G1146300.v1.1 MD05G1223500.v1.1 MD07G1213200.v1.1 MD10G1204900.v1.1 MD10G1205000.v1.1
prunus_persica	Prupe.2G245700_v2.0.a1 Prupe.2G245700_v2.0.a1 Prupe.4G136700_v2.0.a1
pyrus_communis	pycom01g16550 pycom05g20640 pycom07g19600 pycom10g17540
rosa_chinensis	RchiOBHm_Chr1g0369301 RchiOBHm_Chr5g0025831
rosa_laevigata	RLG00000027139 RLG00000032880
rosa_multiflora	Rmu_sc0000968.1_g000014 Rmu_sc0001482.1_g000011
rosa_roxburghii	Rroxscaffold_1G00053370 Rroxscaffold_1G00054370 Rroxscaffold_4G00287750
rosa_rugosa	Rorug01G0349400 Rorug05G0088800 Rorug05G0088900
rosa_samantha	Rh1AG356600 Rh1BG320600 Rh1CG334600 Rh1DG351300 Rh5AG180900 Rh5BG179500 Rh5CG197300 Rh5DG179200
rosa_wichuraiana	Rw1G031480 Rw5G016410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	877
AccB7I	CCANNNNNTGG	1 cut(s)	481
AccI	GTMKAC	1 cut(s)	546
AccIII	TCCGGA	1 cut(s)	466
AciI	CCGC	2 cut(s)	30, 524
AclWI	GGATC	3 cut(s)	515, 1096, 1138
AcoI	YGGCCR	1 cut(s)	584
AcsI	RAATTY	1 cut(s)	784
AcuI	CTGAAG	2 cut(s)	66, 477
AfaI	GTAC	1 cut(s)	851
AfiI	CCNNNNNNNGG	7 cut(s)	53, 262, 263, 481, 497, 530, 957
AflII	CTTAAG	1 cut(s)	421
AflIII	ACRYGT	2 cut(s)	733, 852
AgsI	TTSAA	2 cut(s)	319, 497
AjiI	CACGTC	1 cut(s)	436
AjnI	CCWGG	4 cut(s)	32, 228, 586, 1136
AleI	CACNNNNGTG	1 cut(s)	926
Alw26I	GTCTC	2 cut(s)	612, 737
AlwI	GGATC	3 cut(s)	515, 1096, 1138
Ama87I	CYCGRG	1 cut(s)	779
Aor13HI	TCCGGA	1 cut(s)	466
AoxI	GGCC	5 cut(s)	6, 112, 417, 487, 584
ApeKI	GCWGC	7 cut(s)	21, 50, 376, 629, 632, 1163, 1166
ApoI	RAATTY	1 cut(s)	784
Asp700I	GAANNNNTTC	2 cut(s)	331, 462
AspLEI	GCGC	1 cut(s)	40
AspS9I	GGNCC	3 cut(s)	112, 488, 650
AsuC2I	CCSGG	4 cut(s)	257, 690, 780, 781
AsuHPI	GGTGA	2 cut(s)	59, 770
AsuII	TTCGAA	1 cut(s)	1113
AvaI	CYCGRG	1 cut(s)	779
AvaII	GGWCC	1 cut(s)	650
BalI	TGGCCA	1 cut(s)	586
BanI	GGYRCC	1 cut(s)	877
BauI	CACGAG	1 cut(s)	698
BbvCI	CCTCAGC	1 cut(s)	956
BbvI	GCAGC	6 cut(s)	8, 37, 388, 616, 644, 1150
BccI	CCATC	3 cut(s)	103, 524, 1016
BceAI	ACGGC	1 cut(s)	368
BciT130I	CCWGG	4 cut(s)	34, 230, 588, 1138
BclI	TGATCA	3 cut(s)	204, 270, 825
BcnI	CCSGG	4 cut(s)	257, 690, 780, 781
BcoDI	GTCTC	2 cut(s)	612, 737
BfaI	CTAG	2 cut(s)	654, 1040
BfmI	CTRYAG	2 cut(s)	630, 1164
BfoI	RGCGCY	1 cut(s)	41
BfrI	CTTAAG	1 cut(s)	421
BisI	GCNGC	7 cut(s)	22, 51, 377, 630, 633, 1164, 1167
BlsI	GCNGC	7 cut(s)	23, 52, 378, 631, 634, 1165, 1168
Bme1390I	CCNGG	8 cut(s)	34, 230, 257, 588, 690, 780, 781, 1138
Bme18I	GGWCC	1 cut(s)	650
BmeT110I	CYCGRG	1 cut(s)	779
BmgBI	CACGTC	1 cut(s)	436
BmgT120I	GGNCC	3 cut(s)	112, 488, 650
BmiI	GGNNCC	5 cut(s)	93, 465, 535, 694, 879
BmrFI	CCNGG	8 cut(s)	34, 230, 257, 588, 690, 780, 781, 1138
BmrI	ACTGGG	2 cut(s)	485, 612
BmuI	ACTGGG	2 cut(s)	485, 612
BpmI	CTGGAG	3 cut(s)	251, 520, 1026
Bpu10I	CCTNAGC	1 cut(s)	956
Bpu14I	TTCGAA	1 cut(s)	1113
BpuEI	CTTGAG	1 cut(s)	155
BpuMI	CCSGG	4 cut(s)	257, 690, 780, 781
BsaAI	YACGTR	1 cut(s)	838
BsaBI	GATNNNNATC	1 cut(s)	888
BsaI	GGTCTC	1 cut(s)	612
BsaJI	CCNNGG	8 cut(s)	33, 108, 255, 384, 587, 636, 688, 779
BsaWI	WCCGGW	2 cut(s)	466, 866
BsaXI	ACNNNNNCTCC	4 cut(s)	477, 507, 861, 891
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 262, 263, 481, 497, 530, 957
Bse118I	RCCGGY	2 cut(s)	46, 53
Bse1I	ACTGG	2 cut(s)	491, 607
Bse8I	GATNNNNATC	1 cut(s)	888
BseAI	TCCGGA	1 cut(s)	466
BseBI	CCWGG	4 cut(s)	34, 230, 588, 1138
BseDI	CCNNGG	8 cut(s)	33, 108, 255, 384, 587, 636, 688, 779
BseGI	GGATG	2 cut(s)	26, 1133
BseJI	GATNNNNATC	1 cut(s)	888
BseLI	CCNNNNNNNGG	7 cut(s)	53, 262, 263, 481, 497, 530, 957
BseMII	CTCAG	2 cut(s)	947, 1088
BseNI	ACTGG	2 cut(s)	491, 607
BseRI	GAGGAG	1 cut(s)	248
BseXI	GCAGC	6 cut(s)	8, 37, 388, 616, 644, 1150
BshFI	GGCC	5 cut(s)	8, 114, 419, 489, 586
BshNI	GGYRCC	1 cut(s)	877
BsiHKCI	CYCGRG	1 cut(s)	779
BsiSI	CCGG	7 cut(s)	47, 54, 257, 467, 690, 780, 867
BslFI	GGGAC	2 cut(s)	279, 580
BslI	CCNNNNNNNGG	7 cut(s)	53, 262, 263, 481, 497, 530, 957
BsmAI	GTCTC	2 cut(s)	612, 737
BsmFI	GGGAC	2 cut(s)	279, 580
BsmI	GAATGC	2 cut(s)	73, 1006
BsnI	GGCC	5 cut(s)	8, 114, 419, 489, 586
Bso31I	GGTCTC	1 cut(s)	612
BsoBI	CYCGRG	1 cut(s)	779
Bsp119I	TTCGAA	1 cut(s)	1113
Bsp13I	TCCGGA	1 cut(s)	466
Bsp143I	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
Bsp19I	CCATGG	1 cut(s)	636
BspACI	CCGC	2 cut(s)	30, 524
BspANI	GGCC	5 cut(s)	8, 114, 419, 489, 586
BspCNI	CTCAG	2 cut(s)	948, 1087
BspEI	TCCGGA	1 cut(s)	466
BspLI	GGNNCC	5 cut(s)	93, 465, 535, 694, 879
BspMAI	CTGCAG	2 cut(s)	634, 1168
BspPI	GGATC	3 cut(s)	515, 1096, 1138
BspT104I	TTCGAA	1 cut(s)	1113
BspT107I	GGYRCC	1 cut(s)	877
BspTI	CTTAAG	1 cut(s)	421
BspTNI	GGTCTC	1 cut(s)	612
BsrFI	RCCGGY	2 cut(s)	46, 53
BsrI	ACTGG	2 cut(s)	491, 607
BssAI	RCCGGY	2 cut(s)	46, 53
BssECI	CCNNGG	8 cut(s)	33, 108, 255, 384, 587, 636, 688, 779
BssMI	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
BssSI	CACGAG	1 cut(s)	698
BssT1I	CCWWGG	2 cut(s)	108, 636
Bst2BI	CACGAG	1 cut(s)	698
Bst2UI	CCWGG	4 cut(s)	34, 230, 588, 1138
Bst4CI	ACNGT	2 cut(s)	192, 562
Bst6I	CTCTTC	3 cut(s)	137, 453, 610
BstAFI	CTTAAG	1 cut(s)	421
BstAPI	GCANNNNNTGC	1 cut(s)	1036
BstBAI	YACGTR	1 cut(s)	838
BstBI	TTCGAA	1 cut(s)	1113
BstC8I	GCNNGC	7 cut(s)	19, 48, 55, 352, 381, 396, 451
BstDEI	CTNAG	2 cut(s)	956, 1074
BstDSI	CCRYGG	2 cut(s)	384, 636
BstF5I	GGATG	2 cut(s)	26, 1133
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	1 cut(s)	40
BstKTI	GATC	8 cut(s)	164, 207, 219, 273, 523, 828, 1091, 1133
BstMAI	GTCTC	2 cut(s)	612, 737
BstMBI	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 111, 1036
BstNI	CCWGG	4 cut(s)	34, 230, 588, 1138
BstNSI	RCATGY	1 cut(s)	737
BstSCI	CCNGG	8 cut(s)	32, 228, 255, 586, 688, 778, 779, 1136
BstSFI	CTRYAG	2 cut(s)	630, 1164
BstSNI	TACGTA	1 cut(s)	838
BstV1I	GCAGC	6 cut(s)	8, 37, 388, 616, 644, 1150
BsuRI	GGCC	5 cut(s)	8, 114, 419, 489, 586
BtgI	CCRYGG	2 cut(s)	384, 636
BtgZI	GCGATG	1 cut(s)	150
BtrI	CACGTC	1 cut(s)	436
BtsCI	GGATG	2 cut(s)	26, 1133
Cac8I	GCNNGC	7 cut(s)	19, 48, 55, 352, 381, 396, 451
CfoI	GCGC	1 cut(s)	40
Cfr10I	RCCGGY	2 cut(s)	46, 53
Cfr13I	GGNCC	3 cut(s)	112, 488, 650
Cfr9I	CCCGGG	1 cut(s)	779
Csp6I	GTAC	1 cut(s)	850
CspCI	CAANNNNNGTGG	2 cut(s)	67, 102
CviAII	CATG	5 cut(s)	200, 289, 481, 637, 734
CviQI	GTAC	1 cut(s)	850
DdeI	CTNAG	2 cut(s)	956, 1074
DpnI	GATC	8 cut(s)	163, 206, 218, 272, 522, 827, 1090, 1132
DpnII	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
EaeI	YGGCCR	1 cut(s)	584
Eam1104I	CTCTTC	3 cut(s)	137, 453, 610
EarI	CTCTTC	3 cut(s)	137, 453, 610
EciI	GGCGGA	1 cut(s)	19
Eco105I	TACGTA	1 cut(s)	838
Eco130I	CCWWGG	2 cut(s)	108, 636
Eco147I	AGGCCT	1 cut(s)	419
Eco31I	GGTCTC	1 cut(s)	612
Eco32I	GATATC	1 cut(s)	933
Eco47I	GGWCC	1 cut(s)	650
Eco57I	CTGAAG	2 cut(s)	66, 477
Eco88I	CYCGRG	1 cut(s)	779
EcoO109I	RGGNCCY	1 cut(s)	112
EcoRII	CCWGG	4 cut(s)	32, 228, 586, 1136
EcoRV	GATATC	1 cut(s)	933
EcoT14I	CCWWGG	2 cut(s)	108, 636
ErhI	CCWWGG	2 cut(s)	108, 636
FaeI	CATG	5 cut(s)	203, 292, 484, 640, 737
FaqI	GGGAC	2 cut(s)	279, 580
FatI	CATG	5 cut(s)	199, 288, 480, 636, 733
FauNDI	CATATG	1 cut(s)	664
FbaI	TGATCA	3 cut(s)	204, 270, 825
FblI	GTMKAC	1 cut(s)	546
Fnu4HI	GCNGC	7 cut(s)	22, 51, 377, 630, 633, 1164, 1167
FokI	GGATG	2 cut(s)	13, 1120
Fsp4HI	GCNGC	7 cut(s)	22, 51, 377, 630, 633, 1164, 1167
FspBI	CTAG	2 cut(s)	654, 1040
GlaI	GCGC	1 cut(s)	39
GluI	GCNGC	7 cut(s)	22, 51, 377, 630, 633, 1164, 1167
GsuI	CTGGAG	3 cut(s)	251, 520, 1026
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	5 cut(s)	8, 114, 419, 489, 586
HapII	CCGG	7 cut(s)	47, 54, 257, 467, 690, 780, 867
HhaI	GCGC	1 cut(s)	40
Hin1II	CATG	5 cut(s)	203, 292, 484, 640, 737
Hin6I	GCGC	1 cut(s)	38
HinP1I	GCGC	1 cut(s)	38
HincII	GTYRAC	3 cut(s)	547, 600, 1057
HindII	GTYRAC	3 cut(s)	547, 600, 1057
HindIII	AAGCTT	1 cut(s)	404
HinfI	GANTC	3 cut(s)	64, 140, 1110
HpaI	GTTAAC	2 cut(s)	600, 1057
HpaII	CCGG	7 cut(s)	47, 54, 257, 467, 690, 780, 867
HphI	GGTGA	2 cut(s)	59, 770
Hpy166II	GTNNAC	4 cut(s)	547, 600, 852, 1057
Hpy188I	TCNGA	6 cut(s)	137, 326, 368, 457, 894, 1109
Hpy188III	TCNNGA	3 cut(s)	467, 713, 809
Hpy8I	GTNNAC	4 cut(s)	547, 600, 852, 1057
HpyAV	CCTTC	5 cut(s)	325, 491, 713, 755, 904
HpyCH4III	ACNGT	2 cut(s)	192, 562
HpyCH4IV	ACGT	4 cut(s)	435, 837, 854, 927
HpyCH4V	TGCA	4 cut(s)	245, 441, 632, 1166
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 111, 1036
HpyF3I	CTNAG	2 cut(s)	956, 1074
HpySE526I	ACGT	4 cut(s)	435, 837, 854, 927
Hsp92II	CATG	5 cut(s)	203, 292, 484, 640, 737
HspAI	GCGC	1 cut(s)	38
Kpn2I	TCCGGA	1 cut(s)	466
KroI	GCCGGC	2 cut(s)	46, 53
KroNI	GCCGGC	2 cut(s)	48, 55
Ksp22I	TGATCA	3 cut(s)	204, 270, 825
KspAI	GTTAAC	2 cut(s)	600, 1057
Kzo9I	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
LmnI	GCTCC	7 cut(s)	45, 97, 110, 131, 539, 982, 1160
Lsp1109I	GCAGC	6 cut(s)	8, 37, 388, 616, 644, 1150
MaeI	CTAG	2 cut(s)	654, 1040
MaeII	ACGT	4 cut(s)	435, 837, 854, 927
MaeIII	GTNAC	2 cut(s)	425, 607
MalI	GATC	8 cut(s)	163, 206, 218, 272, 522, 827, 1090, 1132
MboI	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
MboII	GAAGA	6 cut(s)	124, 470, 627, 698, 701, 1127
MlsI	TGGCCA	1 cut(s)	586
MluCI	AATT	3 cut(s)	358, 784, 897
MluNI	TGGCCA	1 cut(s)	586
MlyI	GAGTC	1 cut(s)	58
MmeI	TCCRAC	2 cut(s)	962, 1138
Mox20I	TGGCCA	1 cut(s)	586
MroI	TCCGGA	1 cut(s)	466
MroNI	GCCGGC	2 cut(s)	46, 53
MroXI	GAANNNNTTC	2 cut(s)	331, 462
MscI	TGGCCA	1 cut(s)	586
MseI	TTAA	6 cut(s)	284, 357, 422, 599, 1056, 1171
MslI	CAYNNNNRTG	1 cut(s)	926
Msp20I	TGGCCA	1 cut(s)	586
MspCI	CTTAAG	1 cut(s)	421
MspI	CCGG	7 cut(s)	47, 54, 257, 467, 690, 780, 867
MspR9I	CCNGG	8 cut(s)	34, 230, 257, 588, 690, 780, 781, 1138
Mva1269I	GAATGC	2 cut(s)	73, 1006
MvaI	CCWGG	4 cut(s)	34, 230, 588, 1138
MwoI	GCNNNNNNNGC	3 cut(s)	14, 111, 1036
NaeI	GCCGGC	2 cut(s)	48, 55
NciI	CCSGG	4 cut(s)	257, 690, 780, 781
NcoI	CCATGG	1 cut(s)	636
NdeI	CATATG	1 cut(s)	664
NdeII	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
NgoMIV	GCCGGC	2 cut(s)	46, 53
NlaIII	CATG	5 cut(s)	203, 292, 484, 640, 737
NlaIV	GGNNCC	5 cut(s)	93, 465, 535, 694, 879
NspI	RCATGY	1 cut(s)	737
NspV	TTCGAA	1 cut(s)	1113
OliI	CACNNNNGTG	1 cut(s)	926
PceI	AGGCCT	1 cut(s)	419
PciI	ACATGT	1 cut(s)	733
PctI	GAATGC	2 cut(s)	73, 1006
PdiI	GCCGGC	2 cut(s)	48, 55
PdmI	GAANNNNTTC	2 cut(s)	331, 462
PfeI	GAWTC	2 cut(s)	140, 1110
PflFI	GACNNNGTC	1 cut(s)	1046
PflMI	CCANNNNNTGG	1 cut(s)	481
PkrI	GCNGC	7 cut(s)	23, 52, 378, 631, 634, 1165, 1168
PleI	GAGTC	1 cut(s)	58
PpsI	GAGTC	1 cut(s)	58
Ppu21I	YACGTR	1 cut(s)	838
PscI	ACATGT	1 cut(s)	733
Psp6I	CCWGG	4 cut(s)	32, 228, 586, 1136
PspGI	CCWGG	4 cut(s)	32, 228, 586, 1136
PspN4I	GGNNCC	5 cut(s)	93, 465, 535, 694, 879
PspPI	GGNCC	3 cut(s)	112, 488, 650
PstI	CTGCAG	2 cut(s)	634, 1168
PsyI	GACNNNGTC	1 cut(s)	1046
RsaI	GTAC	1 cut(s)	851
RsaNI	GTAC	1 cut(s)	850
RseI	CAYNNNNRTG	1 cut(s)	926
SalI	GTCGAC	1 cut(s)	545
SaqAI	TTAA	6 cut(s)	284, 357, 422, 599, 1056, 1171
SatI	GCNGC	7 cut(s)	22, 51, 377, 630, 633, 1164, 1167
Sau3AI	GATC	8 cut(s)	161, 204, 216, 270, 520, 825, 1088, 1130
Sau96I	GGNCC	3 cut(s)	112, 488, 650
SchI	GAGTC	1 cut(s)	58
ScrFI	CCNGG	8 cut(s)	34, 230, 257, 588, 690, 780, 781, 1138
SfcI	CTRYAG	2 cut(s)	630, 1164
SfuI	TTCGAA	1 cut(s)	1113
SinI	GGWCC	1 cut(s)	650
SmaI	CCCGGG	1 cut(s)	781
SmiMI	CAYNNNNRTG	1 cut(s)	926
SmlI	CTYRAG	2 cut(s)	170, 421
SmoI	CTYRAG	2 cut(s)	170, 421
SnaBI	TACGTA	1 cut(s)	838
Sse9I	AATT	3 cut(s)	358, 784, 897
SseBI	AGGCCT	1 cut(s)	419
SsiI	CCGC	2 cut(s)	30, 524
SspMI	CTAG	2 cut(s)	654, 1040
StuI	AGGCCT	1 cut(s)	419
StyD4I	CCNGG	8 cut(s)	32, 228, 255, 586, 688, 778, 779, 1136
StyI	CCWWGG	2 cut(s)	108, 636
TaaI	ACNGT	2 cut(s)	192, 562
TaiI	ACGT	4 cut(s)	438, 840, 857, 930
TaqI	TCGA	4 cut(s)	62, 414, 546, 1113
TasI	AATT	3 cut(s)	358, 784, 897
TatI	WGTACW	1 cut(s)	849
TfiI	GAWTC	2 cut(s)	140, 1110
Tru1I	TTAA	6 cut(s)	284, 357, 422, 599, 1056, 1171
Tru9I	TTAA	6 cut(s)	284, 357, 422, 599, 1056, 1171
TseI	GCWGC	7 cut(s)	21, 50, 376, 629, 632, 1163, 1166
TspDTI	ATGAA	1 cut(s)	732
TspGWI	ACGGA	1 cut(s)	1143
TspMI	CCCGGG	1 cut(s)	779
Tth111I	GACNNNGTC	1 cut(s)	1046
Van91I	CCANNNNNTGG	1 cut(s)	481
Vha464I	CTTAAG	1 cut(s)	421
VpaK11BI	GGWCC	1 cut(s)	650
XapI	RAATTY	1 cut(s)	784
XceI	RCATGY	1 cut(s)	737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	85
XmaI	CCCGGG	1 cut(s)	779
XmiI	GTMKAC	1 cut(s)	546
XmnI	GAANNNNTTC	2 cut(s)	331, 462
XspI	CTAG	2 cut(s)	654, 1040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.