MD01G1155800.v1.1
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008985
Physical Location & Seq
Forward (+)
26372859 .. 26373998
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1155800.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGACCGCCCGACCCGTACCCACGCCCCGGCAGGCCGCGACGATTGTTGGAGTGAGGACGCCACGTTGGCACTAACAACGGCCTGGGGCGACCGCTACCTCCATTTAAACCGCGGCATCCTCCGTCAGAAAGACTGGAAGGAGGTGGCCGACGCCGTTAACTCCCATCAGAACGGCGGCGCCAAGCCGTTCAAGACGGACGTCCAGTGCAAGAACCGCATCGACACGTTGAAGAAAAAGTACAAGCTGGAAAAGTCGAAGCCCGGCCCGTCCACGTGGCCCTTCTACCGCCGCCTCCACTCCATAATCTGCTCAACCCCCTCAAACACCGCCGGTGCGACCAAACCAAACCCCCCAATCCTCGCTCTCACTGCCAAATCCCCCGACCCGAATACGAATCCCCTCGGAGGGTCCACCGACAGCTCAATTCGCCGCGACGACGGCGGTGATGAGGCGGCGGTGTTTTCGGGTGGGGCGGCGTGCAGGGAATTGGCTAGGGCAATTTCGAAGTTTGGGGAAATGTACGAGAGGATTGAGAATTCGAAGCGGAAGCAGATGATGGAGCTGGAGAAACAGAGGATGGAGTTCGAGAAGGAGCTCGCGTTGCAGAGGTTGAATATGTTCATGGATGTTCAGGTGGAGCTGGGGAAGAAGATGAAGCGCCCCAAGTACTCGCATTCTTCAGGGTCGGTTGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_4

Protein Analysis

234

Amino Acids

26.22

Weight (kDa)

9.44

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 15 - 102 2.6e-18 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 4 cut(s) 39, 114, 435, 602
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 666
AcvI CACGTG 1 cut(s) 276
AcyI GRCGYC 4 cut(s) 60, 153, 180, 201
AfaI GTAC 4 cut(s) 19, 242, 524, 671
AfiI CCNNNNNNNGG 3 cut(s) 28, 407, 408
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 193, 232, 616
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 5 cut(s) 247, 423, 565, 598, 643
AluI AGCT 5 cut(s) 247, 423, 565, 598, 643
Alw21I GWGCWC 1 cut(s) 600
AoxI GGCC 5 cut(s) 34, 81, 147, 265, 278
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 620
AspLEI GCGC 2 cut(s) 182, 663
AspS9I GGNCC 4 cut(s) 4, 266, 279, 411
AsuC2I CCSGG 2 cut(s) 29, 264
AsuHPI GGTGA 1 cut(s) 458
AsuII TTCGAA 2 cut(s) 506, 542
AvaII GGWCC 2 cut(s) 4, 411
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 600
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
BbrPI CACGTG 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 600
BccI CCATC 3 cut(s) 174, 553, 574
BceAI ACGGC 5 cut(s) 96, 140, 172, 190, 457
BciT130I CCWGG 1 cut(s) 85
BcnI CCSGG 2 cut(s) 29, 264
BfaI CTAG 1 cut(s) 495
BfoI RGCGCY 2 cut(s) 183, 664
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 7 cut(s) 37, 115, 178, 292, 433, 456, 477
BlsI GCNGC 7 cut(s) 38, 116, 179, 293, 434, 457, 478
BmcAI AGTACT 1 cut(s) 671
Bme1390I CCNGG 3 cut(s) 29, 85, 264
Bme18I GGWCC 2 cut(s) 4, 411
BmgT120I GGNCC 4 cut(s) 4, 266, 279, 411
BmiI GGNNCC 2 cut(s) 181, 412
BmrFI CCNGG 3 cut(s) 29, 85, 264
BmsI GCATC 2 cut(s) 126, 228
BoxI GACNNNNGTC 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 587
Bpu14I TTCGAA 2 cut(s) 506, 542
BpuMI CCSGG 2 cut(s) 29, 264
BsaAI YACGTR 1 cut(s) 276
BsaHI GRCGYC 4 cut(s) 60, 153, 180, 201
BsaJI CCNNGG 4 cut(s) 27, 84, 112, 403
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 407, 408
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 2 cut(s) 140, 205
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 4 cut(s) 27, 84, 112, 403
BseGI GGATG 3 cut(s) 117, 585, 634
BseLI CCNNNNNNNGG 3 cut(s) 28, 407, 408
BseNI ACTGG 2 cut(s) 140, 205
BseYI CCCAGC 1 cut(s) 643
BsgI GTGCAG 1 cut(s) 502
Bsh1236I CGCG 4 cut(s) 39, 114, 435, 602
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 5 cut(s) 36, 83, 149, 267, 280
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 600
BsiSI CCGG 3 cut(s) 29, 264, 333
BslI CCNNNNNNNGG 3 cut(s) 28, 407, 408
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 5 cut(s) 36, 83, 149, 267, 280
Bsp119I TTCGAA 2 cut(s) 506, 542
Bsp1286I GDGCHC 1 cut(s) 600
BspANI GGCC 5 cut(s) 36, 83, 149, 267, 280
BspFNI CGCG 4 cut(s) 39, 114, 435, 602
BspLI GGNNCC 2 cut(s) 181, 412
BspT104I TTCGAA 2 cut(s) 506, 542
BspT107I GGYRCC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 2 cut(s) 140, 205
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 4 cut(s) 27, 84, 112, 403
BssNI GRCGYC 4 cut(s) 60, 153, 180, 201
Bst2UI CCWGG 1 cut(s) 85
BstACI GRCGYC 4 cut(s) 60, 153, 180, 201
BstBAI YACGTR 1 cut(s) 276
BstBI TTCGAA 2 cut(s) 506, 542
BstC8I GCNNGC 3 cut(s) 34, 481, 600
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 3 cut(s) 117, 585, 634
BstFNI CGCG 4 cut(s) 39, 114, 435, 602
BstH2I RGCGCY 2 cut(s) 183, 664
BstHHI GCGC 2 cut(s) 182, 663
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 6 cut(s) 68, 216, 371, 429, 441, 604
BstNI CCWGG 1 cut(s) 85
BstPAI GACNNNNGTC 1 cut(s) 200
BstSCI CCNGG 3 cut(s) 27, 83, 262
BstUI CGCG 4 cut(s) 39, 114, 435, 602
BsuRI GGCC 5 cut(s) 36, 83, 149, 267, 280
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 117, 585, 634
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 212, 369
Cac8I GCNNGC 3 cut(s) 34, 481, 600
CfoI GCGC 2 cut(s) 182, 663
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 4 cut(s) 4, 266, 279, 411
Cfr42I CCGCGG 1 cut(s) 115
CseI GACGC 2 cut(s) 68, 161
Csp6I GTAC 4 cut(s) 18, 241, 523, 670
CviAII CATG 1 cut(s) 625
CviQI GTAC 4 cut(s) 18, 241, 523, 670
DinI GGCGCC 1 cut(s) 181
DraI TTTAAA 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 4, 411
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 600
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
FaeI CATG 1 cut(s) 628
FaiI YATR 3 cut(s) 305, 620, 626
FatI CATG 1 cut(s) 624
Fnu4HI GCNGC 7 cut(s) 37, 115, 178, 292, 433, 456, 477
FokI GGATG 3 cut(s) 104, 592, 641
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 7 cut(s) 37, 115, 178, 292, 433, 456, 477
FspBI CTAG 1 cut(s) 495
GlaI GCGC 2 cut(s) 181, 662
GluI GCNGC 7 cut(s) 37, 115, 178, 292, 433, 456, 477
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 587
HaeII RGCGCY 2 cut(s) 183, 664
HaeIII GGCC 5 cut(s) 36, 83, 149, 267, 280
HapII CCGG 3 cut(s) 29, 264, 333
HgaI GACGC 2 cut(s) 68, 161
HhaI GCGC 2 cut(s) 182, 663
Hin1I GRCGYC 4 cut(s) 60, 153, 180, 201
Hin1II CATG 1 cut(s) 628
Hin6I GCGC 2 cut(s) 180, 661
HinP1I GCGC 2 cut(s) 180, 661
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 1 cut(s) 397
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 3 cut(s) 29, 264, 333
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 3 cut(s) 160, 273, 414
Hpy188I TCNGA 3 cut(s) 129, 171, 407
Hpy188III TCNNGA 2 cut(s) 193, 589
Hpy8I GTNNAC 3 cut(s) 160, 273, 414
Hpy99I CGWCG 4 cut(s) 44, 155, 440, 443
HpyAV CCTTC 3 cut(s) 133, 292, 586
HpyCH4IV ACGT 4 cut(s) 65, 201, 227, 275
HpyCH4V TGCA 3 cut(s) 210, 483, 607
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 216, 371, 429, 441, 604
HpySE526I ACGT 4 cut(s) 65, 201, 227, 275
Hsp92I GRCGYC 4 cut(s) 60, 153, 180, 201
Hsp92II CATG 1 cut(s) 628
HspAI GCGC 2 cut(s) 180, 661
KasI GGCGCC 1 cut(s) 179
KspAI GTTAAC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 115
LmnI GCTCC 3 cut(s) 562, 595, 640
LweI GCATC 2 cut(s) 126, 228
MaeI CTAG 1 cut(s) 495
MaeII ACGT 4 cut(s) 65, 201, 227, 275
MboII GAAGA 4 cut(s) 244, 661, 664, 672
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 4 cut(s) 426, 488, 501, 538
Mly113I GGCGCC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 620
MseI TTAA 3 cut(s) 107, 159, 700
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 3 cut(s) 29, 264, 333
MspR9I CCNGG 3 cut(s) 29, 85, 264
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 1 cut(s) 85
MvnI CGCG 4 cut(s) 39, 114, 435, 602
MwoI GCNNNNNNNGC 6 cut(s) 68, 216, 371, 429, 441, 604
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 2 cut(s) 29, 264
NlaIII CATG 1 cut(s) 628
NlaIV GGNNCC 2 cut(s) 181, 412
NspV TTCGAA 2 cut(s) 506, 542
PctI GAATGC 1 cut(s) 676
PdmI GAANNNNTTC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 7 cut(s) 38, 116, 179, 293, 434, 457, 478
PluTI GGCGCC 1 cut(s) 183
PmaCI CACGTG 1 cut(s) 276
PmlI CACGTG 1 cut(s) 276
Ppu21I YACGTR 1 cut(s) 276
PshAI GACNNNNGTC 1 cut(s) 200
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 83
PspCI CACGTG 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 2 cut(s) 181, 412
PspPI GGNCC 4 cut(s) 4, 266, 279, 411
RsaI GTAC 4 cut(s) 19, 242, 524, 671
RsaNI GTAC 4 cut(s) 18, 241, 523, 670
SacI GAGCTC 1 cut(s) 600
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 3 cut(s) 107, 159, 700
SatI GCNGC 7 cut(s) 37, 115, 178, 292, 433, 456, 477
Sau96I GGNCC 4 cut(s) 4, 266, 279, 411
ScaI AGTACT 1 cut(s) 671
ScrFI CCNGG 3 cut(s) 29, 85, 264
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 2 cut(s) 126, 228
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 115
SfuI TTCGAA 2 cut(s) 506, 542
SgrAI CRCCGGYG 1 cut(s) 332
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 2 cut(s) 4, 411
Sse9I AATT 4 cut(s) 426, 488, 501, 538
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 3 cut(s) 27, 83, 262
TaiI ACGT 4 cut(s) 68, 204, 230, 278
TaqI TCGA 5 cut(s) 222, 257, 506, 542, 588
TasI AATT 4 cut(s) 426, 488, 501, 538
TatI WGTACW 2 cut(s) 240, 669
TauI GCSGC 7 cut(s) 39, 117, 180, 294, 435, 458, 479
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 3 cut(s) 107, 159, 700
Tru9I TTAA 3 cut(s) 107, 159, 700
TscAI CASTG 2 cut(s) 212, 376
TspDTI ATGAA 2 cut(s) 613, 671
TspGWI ACGGA 2 cut(s) 113, 212
TspRI CASTG 2 cut(s) 212, 376
VpaK11BI GGWCC 2 cut(s) 4, 411
XapI RAATTY 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 1 cut(s) 495
ZraI GACGTC 1 cut(s) 202
ZrmI AGTACT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.