MD01G1198200.v1.1

Plant organelle RNA recognition domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009357
Physical Location & Seq
Forward (+)
29606194 .. 29607540
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1198200.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGCGCATATTCTTAACCTACTCATCCAATTTCTTGAACCCCAAGTCAAAAACCCTAAACCCCATCACTTTCCTCCTCTGCAATCTCACCCCGACCCGACCCAAATCGCAGTCGACCTCCATCCCCAGAAAACAAGCCAGAATCCGCGACCACGGCTACGACAACTACATGGAAATCGAAAAGAAAACCCGAAAAGTCCTCAAATTTCAGGACCTCATCCTGTCGCAGCCCAACCAAATCATCCAAGTCTCCCGCCTCGACCTCCTCGCCCGCCGCTTCGGCTTCAAGCAGCACGAGGCCGGCGTTTTCGTGCTCAAATTCCCCCACGTTTTCGAGATTTACGAGCACCCGGTTCAGCGGATCCTCTACTGCCGGTTGACCCGAAAAGCCCACCTCCAGATTGAGCAGGAGAAGCAGGCGCTGGTGGCACAAACTCCTGACGCCGTCACCCGCCTGAGAAAGCTCCTGATGATGTCCAACACCGGGCGGCTCCGCCTCGAGCATGTTCGGATTGCGAGGGCGGAGTTTGGGCTGCCGGATGATTTCGAGTACTCGGTAATTCTCAAACACCCTGAATACTTTAGATTGTTTGATGCTGAGGAAACTAGGAACAAGTACATTGAGGTTGTTGAGAAAGATTGTAGCTTATCTTCTTGTGCAATTGAGAAACTTAGGGAGATTGAGTATAGGGAGAGAGGGATTGATGCCGAGGATATAAGGTTTTCGTTTATTGTGAATTTTCCACCCGGGTTTAAGATTGGGAAGTACTATAGGATTGCCGTCTGGAAATGGCAGAGACTTCCGTATTGGTCTCCGTACGAGGATGTTTCTGGTTATGACTTGAGGTCGATTGAGGCGCAGAAGCGGATGGAGAAGAGAGCGGTCGCTGTGATTCATGAACTGCTGTCGTTGACGGTGGAGAAGAAGATCACATTGGAGAGGATTGCTCATTTTAGAATGGTGATGAATTTGCCGAATAAGTTGAAAGAGTTTCTGCTTCAGCATCAGGGGATTTTCTATATTTCAACTAGAGGGAATCACGGGAAGCTTCATACGGTGTTTCTAAGGGAGGCTTATAAGAAGGGTGAGTTGATAGAACCAAATGATTTGTATTTGGCGAGAAGGAAATTGGCTGAGTTGGTTTTGATTAGCCCTAGGAAGGCGAGAATGGACAGCGAATTGGTTAGTTACCACAGAGATTGGGAAGATGATGAGATGGGGCACATTGGAAGAGAACGCATTGTGAATGGTTTTGAGGATTTAGGAGGCGGGGATGATGTTAGGCGAGAGAGGGATGTGGAGGGTGGTACGAAATCAGATAGTGGTAGTAATTATGACTCTGATTGA

Protein Analysis

449

Amino Acids

52.76

Weight (kDa)

9.29

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 49 - 383 9.2e-112 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1077
Acc16I TGCGCA 1 cut(s) 5
AccBSI CCGCTC 1 cut(s) 881
AccI GTMKAC 1 cut(s) 113
AccII CGCG 1 cut(s) 147
AclWI GGATC 2 cut(s) 355, 368
AcsI RAATTY 4 cut(s) 203, 317, 736, 967
AcuI CTGAAG 1 cut(s) 983
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 5 cut(s) 551, 617, 767, 818, 1309
AfiI CCNNNNNNNGG 2 cut(s) 483, 1159
AgsI TTSAA 4 cut(s) 37, 286, 985, 1026
AhdI GACNNNNNGTC 1 cut(s) 844
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AluBI AGCT 3 cut(s) 463, 645, 1048
AluI AGCT 3 cut(s) 463, 645, 1048
Alw21I GWGCWC 2 cut(s) 315, 348
Alw26I GTCTC 3 cut(s) 253, 790, 816
AlwI GGATC 2 cut(s) 355, 368
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 2 cut(s) 497, 746
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 3 cut(s) 226, 289, 532
ApoI RAATTY 4 cut(s) 203, 317, 736, 967
AspA2I CCTAGG 1 cut(s) 1154
AspLEI GCGC 3 cut(s) 6, 421, 859
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 4 cut(s) 350, 484, 747, 748
AsuHPI GGTGA 4 cut(s) 79, 439, 973, 1097
AvaI CYCGRG 2 cut(s) 497, 746
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 1154
BaeGI GKGCMC 1 cut(s) 1224
BamHI GGATCC 1 cut(s) 360
BarI GAAGNNNNNNTAC 2 cut(s) 634, 666
BauI CACGAG 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 315, 348
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 238, 301, 519
BccI CCATC 4 cut(s) 71, 128, 862, 1210
BceAI ACGGC 3 cut(s) 169, 428, 764
BcnI CCSGG 4 cut(s) 350, 484, 747, 748
BcoDI GTCTC 3 cut(s) 253, 790, 816
BfaI CTAG 3 cut(s) 606, 1029, 1155
BfmI CTRYAG 1 cut(s) 769
BfoI RGCGCY 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 279
BisI GCNGC 5 cut(s) 227, 274, 290, 488, 533
BlnI CCTAGG 1 cut(s) 1154
BlsI GCNGC 5 cut(s) 228, 275, 291, 489, 534
BmcAI AGTACT 2 cut(s) 551, 767
Bme1390I CCNGG 4 cut(s) 350, 484, 747, 748
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 844
BmeT110I CYCGRG 2 cut(s) 497, 746
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 362, 491
BmrFI CCNGG 4 cut(s) 350, 484, 747, 748
BmsI GCATC 3 cut(s) 583, 694, 1012
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 862
BpuMI CCSGG 4 cut(s) 350, 484, 747, 748
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 151, 708, 746, 1154
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 1159
Bse118I RCCGGY 2 cut(s) 299, 372
BseDI CCNNGG 4 cut(s) 151, 708, 746, 1154
BseGI GGATG 9 cut(s) 23, 120, 216, 240, 544, 829, 873, 1279, 1300
BseLI CCNNNNNNNGG 2 cut(s) 483, 1159
BseMII CTCAG 3 cut(s) 446, 588, 1125
BseRI GAGGAG 2 cut(s) 65, 254
BseSI GKGCMC 1 cut(s) 1224
BseXI GCAGC 3 cut(s) 238, 301, 519
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 885
BshFI GGCC 1 cut(s) 299
BsiEI CGRYCG 1 cut(s) 885
BsiHKAI GWGCWC 2 cut(s) 315, 348
BsiHKCI CYCGRG 2 cut(s) 497, 746
BsiSI CCGG 6 cut(s) 300, 350, 373, 483, 536, 747
BsiWI CGTACG 1 cut(s) 816
BslI CCNNNNNNNGG 2 cut(s) 483, 1159
BsmAI GTCTC 3 cut(s) 253, 790, 816
BsnI GGCC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 816
BsoBI CYCGRG 2 cut(s) 497, 746
Bsp1286I GDGCHC 3 cut(s) 315, 348, 1224
Bsp143I GATC 2 cut(s) 360, 927
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 3 cut(s) 447, 589, 1126
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 2 cut(s) 362, 491
BspPI GGATC 2 cut(s) 355, 368
BspTNI GGTCTC 1 cut(s) 816
BsrBI CCGCTC 1 cut(s) 881
BsrFI RCCGGY 2 cut(s) 299, 372
BssAI RCCGGY 2 cut(s) 299, 372
BssECI CCNNGG 4 cut(s) 151, 708, 746, 1154
BssMI GATC 2 cut(s) 360, 927
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 1154
Bst2BI CACGAG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 916, 1057
Bst6I CTCTTC 2 cut(s) 869, 1225
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 271, 301, 417
BstDEI CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 9 cut(s) 23, 120, 216, 240, 544, 829, 873, 1279, 1300
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 3 cut(s) 6, 421, 859
BstKTI GATC 2 cut(s) 363, 930
BstMAI GTCTC 3 cut(s) 253, 790, 816
BstMBI GATC 2 cut(s) 360, 927
BstMCI CGRYCG 1 cut(s) 885
BstMWI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 4 cut(s) 348, 482, 745, 746
BstSFI CTRYAG 1 cut(s) 769
BstSLI GKGCMC 1 cut(s) 1224
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 238, 301, 519
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 9 cut(s) 23, 120, 216, 240, 544, 829, 873, 1279, 1300
Cac8I GCNNGC 3 cut(s) 271, 301, 417
CaiI CAGNNNCTG 1 cut(s) 421
CciI TCATGA 1 cut(s) 895
CfoI GCGC 3 cut(s) 6, 421, 859
Cfr10I RCCGGY 2 cut(s) 299, 372
Cfr13I GGNCC 1 cut(s) 211
Cfr9I CCCGGG 1 cut(s) 746
CseI GACGC 1 cut(s) 449
Csp6I GTAC 5 cut(s) 550, 616, 766, 817, 1308
CspCI CAANNNNNGTGG 2 cut(s) 1181, 1216
CviAII CATG 3 cut(s) 169, 503, 896
CviQI GTAC 5 cut(s) 550, 616, 766, 817, 1308
DdeI CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
DpnI GATC 2 cut(s) 362, 929
DpnII GATC 2 cut(s) 360, 927
DriI GACNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 2 cut(s) 869, 1225
Eam1105I GACNNNNNGTC 1 cut(s) 844
EarI CTCTTC 2 cut(s) 869, 1225
EciI GGCGGA 2 cut(s) 482, 536
Eco130I CCWWGG 1 cut(s) 1154
Eco31I GGTCTC 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 983
Eco88I CYCGRG 2 cut(s) 497, 746
EcoO109I RGGNCCY 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 1154
ErhI CCWWGG 1 cut(s) 1154
FaeI CATG 3 cut(s) 172, 506, 899
FalI AAGNNNNNCTT 2 cut(s) 1057, 1089
FatI CATG 3 cut(s) 168, 502, 895
FauI CCCGC 4 cut(s) 260, 278, 458, 1262
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 5 cut(s) 227, 274, 290, 488, 533
FokI GGATG 9 cut(s) 10, 107, 203, 227, 551, 836, 880, 1286, 1307
Fsp4HI GCNGC 5 cut(s) 227, 274, 290, 488, 533
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 3 cut(s) 606, 1029, 1155
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 420, 858
GluI GCNGC 5 cut(s) 227, 274, 290, 488, 533
GsuI CTGGAG 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 1 cut(s) 299
HapII CCGG 6 cut(s) 300, 350, 373, 483, 536, 747
HgaI GACGC 1 cut(s) 449
HhaI GCGC 3 cut(s) 6, 421, 859
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 3 cut(s) 172, 506, 899
Hin6I GCGC 3 cut(s) 4, 419, 857
HinP1I GCGC 3 cut(s) 4, 419, 857
HincII GTYRAC 3 cut(s) 114, 378, 912
HindII GTYRAC 3 cut(s) 114, 378, 912
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 4 cut(s) 141, 892, 1036, 1337
HpaII CCGG 6 cut(s) 300, 350, 373, 483, 536, 747
HphI GGTGA 4 cut(s) 79, 439, 973, 1097
Hpy166II GTNNAC 3 cut(s) 114, 378, 912
Hpy188I TCNGA 3 cut(s) 510, 1318, 1342
Hpy188III TCNNGA 8 cut(s) 34, 209, 334, 397, 437, 466, 784, 896
Hpy8I GTNNAC 3 cut(s) 114, 378, 912
HpyAV CCTTC 3 cut(s) 1075, 1116, 1153
HpyCH4III ACNGT 2 cut(s) 916, 1057
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 81, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
HpyF3I CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 3 cut(s) 172, 506, 899
HspAI GCGC 3 cut(s) 4, 419, 857
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 360, 927
LmnI GCTCC 2 cut(s) 468, 495
Lsp1109I GCAGC 3 cut(s) 238, 301, 519
LweI GCATC 3 cut(s) 583, 694, 1012
MaeI CTAG 3 cut(s) 606, 1029, 1155
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 445, 1187
MalI GATC 2 cut(s) 362, 929
MbiI CCGCTC 1 cut(s) 881
MboI GATC 2 cut(s) 360, 927
MboII GAAGA 6 cut(s) 642, 886, 934, 937, 1217, 1242
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 3 cut(s) 315, 348, 1224
MlyI GAGTC 1 cut(s) 1331
MmeI TCCRAC 1 cut(s) 501
MroNI GCCGGC 1 cut(s) 299
MseI TTAA 2 cut(s) 14, 753
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 6 cut(s) 300, 350, 373, 483, 536, 747
MspR9I CCNGG 4 cut(s) 350, 484, 747, 748
MunI CAATTG 1 cut(s) 660
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 4 cut(s) 350, 484, 747, 748
NdeII GATC 2 cut(s) 360, 927
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 3 cut(s) 172, 506, 899
NlaIV GGNNCC 2 cut(s) 362, 491
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 895
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 339
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 3 cut(s) 141, 892, 1036
Pfl23II CGTACG 1 cut(s) 816
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 5 cut(s) 228, 275, 291, 489, 534
PleI GAGTC 1 cut(s) 1331
PpsI GAGTC 1 cut(s) 1331
PpuMI RGGWCCY 1 cut(s) 211
PsiI TTATAA 1 cut(s) 1077
Psp5II RGGWCCY 1 cut(s) 211
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 2 cut(s) 362, 491
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PspXI VCTCGAGB 1 cut(s) 497
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 360
PsyI GACNNNGTC 1 cut(s) 443
RsaI GTAC 5 cut(s) 551, 617, 767, 818, 1309
RsaNI GTAC 5 cut(s) 550, 616, 766, 817, 1308
SalI GTCGAC 1 cut(s) 112
SaqAI TTAA 2 cut(s) 14, 753
SatI GCNGC 5 cut(s) 227, 274, 290, 488, 533
Sau3AI GATC 2 cut(s) 360, 927
Sau96I GGNCC 1 cut(s) 211
ScaI AGTACT 2 cut(s) 551, 767
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 4 cut(s) 350, 484, 747, 748
SduI GDGCHC 3 cut(s) 315, 348, 1224
SfaNI GCATC 3 cut(s) 583, 694, 1012
SfcI CTRYAG 1 cut(s) 769
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 497
SmaI CCCGGG 1 cut(s) 748
SmlI CTYRAG 2 cut(s) 497, 841
SmoI CTYRAG 2 cut(s) 497, 841
SspMI CTAG 3 cut(s) 606, 1029, 1155
StyD4I CCNGG 4 cut(s) 348, 482, 745, 746
StyI CCWWGG 1 cut(s) 1154
TaaI ACNGT 2 cut(s) 916, 1057
TaiI ACGT 1 cut(s) 330
TaqI TCGA 7 cut(s) 113, 177, 258, 333, 498, 546, 848
TatI WGTACW 3 cut(s) 549, 615, 765
TauI GCSGC 2 cut(s) 276, 490
TfiI GAWTC 3 cut(s) 141, 892, 1036
Tru1I TTAA 2 cut(s) 14, 753
Tru9I TTAA 2 cut(s) 14, 753
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 3 cut(s) 226, 289, 532
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 4 cut(s) 884, 912, 980, 1040
TspGWI ACGGA 2 cut(s) 792, 804
TspMI CCCGGG 1 cut(s) 746
Tth111I GACNNNGTC 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 4 cut(s) 203, 317, 736, 967
XceI RCATGY 1 cut(s) 506
XhoI CTCGAG 1 cut(s) 497
XmaI CCCGGG 1 cut(s) 746
XmaJI CCTAGG 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 3 cut(s) 606, 1029, 1155
ZrmI AGTACT 2 cut(s) 551, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.