pycom01g20830

Plant organelle RNA recognition domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009357
Physical Location & Seq
Forward (+)
19149590 .. 19150936
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20830.1

Sequence Viewer

Length: 1347 bp
ATGCGCATTTTCTTAACCTACTCATCCAATTTCTTGAACCCCAAGCCAAAAACCCTAAACCCCATCACTTTCCTCCTCTGCAATCTCACCCCGACCCGACCCAAATCGCAGTCGACCTCCATCCCCAGAAAACAAGCCAGAATCCGCGACCACGGCTACGACAACTACATGGAAATCGAAAAGAAAACCCGAAAAGTCCTCAAATTTCAGGACCTCATCCTGTCGCAACCCAACCAAATCATCCAAGTCTCCCGCCTCGACCTCCTCGCCCGCCGCTTCGGCTTCAAGCAACACGAGGCCGGCGTTTTCGTGCTCAAATTCCCCCACGTCTTCGAGATTTACGAGCACCCGGTTCAGCGGATCCTCTACTGCCGGTTGACCCGAAAAGCCCACCTCCAGATTGAGCAGGAGAAGCAGGCGCTGGTGGCACAAACTCCTGACGCCGTCACCCGCCTGAGAAAGCTCCTGATGATGTCCAACACGGGGCGGCTCCGCCTCGAGCATGTTCGGATTGCGAGGGCGGAGTTTGGGCTGCCGGATGATTTCGAGTACTCGGTAATTCTCAAACACCCTGAATACTTTAGATTGTTTGATGCTGAGGAAACTAGGAACAAGTACATTGAGGTTGTTGAGAAAGATTGTAGCTTATCTGTTTGTGCAATTGAGAAACTTAGGGAGATTGAGTATAGGGGGAGAGGGATTGATGCCGAGGATATAAGGTTTTCGTTTATTGTGAATTTTCCACCCGGTTTTAAGATTGGGAAGTACTATCGGATTGCCGTGTGGAAATGGCAGAGACTTCCCTATTGGTCTCCGTACGAGGATATTTCCGGTTATGACTTGAGGTCGATTGAGGCGCAGAAGCGGATGGAGAAGAGAGCAGTTGCTGTGATTCATGAATTGCTGTCGTTGACGGTGGAGAAGAAGAGCACATTGGAGAGGATTGCTCATTTTAGAATGGCGATGAATTTGCCTAATAAGTTGAAAGAGTTTCTGCTTCAGCATCAGGGGATTTTCTATATTTCAACTAGAGGGAATCATGGGAAGCTTCACACGGTGTTTCTCAGGGAGGCTTATAAGAAGGGGGAGTTGATAGAGCCAAATGATTTGTATTTGGCGAGAAGGAAATTGGCTGAGTTGGTTTTGATTAGCCCTAGGAAGGCGAGAATGGATAGCGAATTGGTTAGTTACCGCAGAGATTGGGAAGATGATGAGATGGGGCACATTGGAAGAGAACGCATTGTGAATGCTTTTGAGGATTTAGGAGGTGGGGATGATGTTAGGCGGGAGAGGGATGTGGTGGGTGGTAGAAAATCAGATAATGGTAGTGATTATGACTCTGATTGA

Protein Analysis

449

Amino Acids

52.76

Weight (kDa)

9.44

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 49 - 383 5.3e-112 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1077
Acc16I TGCGCA 1 cut(s) 5
AccI GTMKAC 1 cut(s) 113
AccII CGCG 1 cut(s) 147
AclWI GGATC 2 cut(s) 355, 368
AcsI RAATTY 4 cut(s) 203, 317, 736, 967
AcuI CTGAAG 1 cut(s) 983
AcyI GRCGYC 1 cut(s) 441
AdeI CACNNNGTG 1 cut(s) 1057
AfaI GTAC 4 cut(s) 551, 617, 767, 818
AfiI CCNNNNNNNGG 2 cut(s) 483, 1159
AgsI TTSAA 4 cut(s) 37, 286, 985, 1026
AhdI GACNNNNNGTC 1 cut(s) 844
AjiI CACGTC 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AluBI AGCT 3 cut(s) 463, 645, 1048
AluI AGCT 3 cut(s) 463, 645, 1048
Alw21I GWGCWC 3 cut(s) 315, 348, 932
Alw26I GTCTC 3 cut(s) 253, 790, 816
AlwI GGATC 2 cut(s) 355, 368
AlwNI CAGNNNCTG 2 cut(s) 421, 887
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 1 cut(s) 532
ApoI RAATTY 4 cut(s) 203, 317, 736, 967
AspA2I CCTAGG 1 cut(s) 1154
AspLEI GCGC 3 cut(s) 6, 421, 859
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 2 cut(s) 350, 747
AsuHPI GGTGA 2 cut(s) 79, 439
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 1154
BaeGI GKGCMC 1 cut(s) 1224
BamHI GGATCC 1 cut(s) 360
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 322
Bbv12I GWGCWC 3 cut(s) 315, 348, 932
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 519
BccI CCATC 4 cut(s) 71, 128, 862, 1210
BceAI ACGGC 3 cut(s) 169, 428, 764
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BcnI CCSGG 2 cut(s) 350, 747
BcoDI GTCTC 3 cut(s) 253, 790, 816
BfaI CTAG 3 cut(s) 606, 1029, 1155
BfoI RGCGCY 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 279
BisI GCNGC 3 cut(s) 274, 488, 533
BlnI CCTAGG 1 cut(s) 1154
BlsI GCNGC 3 cut(s) 275, 489, 534
BmcAI AGTACT 2 cut(s) 551, 767
Bme1390I CCNGG 2 cut(s) 350, 747
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 362, 491
BmrFI CCNGG 2 cut(s) 350, 747
BmsI GCATC 3 cut(s) 583, 694, 1012
BpiI GAAGAC 1 cut(s) 322
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 862
BpuMI CCSGG 2 cut(s) 350, 747
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 3 cut(s) 151, 708, 1154
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 1159
Bse118I RCCGGY 2 cut(s) 299, 372
BseDI CCNNGG 3 cut(s) 151, 708, 1154
BseGI GGATG 8 cut(s) 23, 120, 216, 240, 544, 873, 1279, 1300
BseLI CCNNNNNNNGG 2 cut(s) 483, 1159
BseMII CTCAG 4 cut(s) 446, 588, 1078, 1125
BseRI GAGGAG 2 cut(s) 65, 254
BseSI GKGCMC 1 cut(s) 1224
BseXI GCAGC 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 299
BsiHKAI GWGCWC 3 cut(s) 315, 348, 932
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 6 cut(s) 300, 350, 373, 536, 747, 831
BsiWI CGTACG 1 cut(s) 816
BslI CCNNNNNNNGG 2 cut(s) 483, 1159
BsmAI GTCTC 3 cut(s) 253, 790, 816
BsmI GAATGC 1 cut(s) 1252
BsnI GGCC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 816
BsoBI CYCGRG 1 cut(s) 497
Bsp1286I GDGCHC 4 cut(s) 315, 348, 932, 1224
Bsp143I GATC 1 cut(s) 360
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 4 cut(s) 447, 589, 1077, 1126
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 2 cut(s) 362, 491
BspPI GGATC 2 cut(s) 355, 368
BspQI GCTCTTC 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 299, 372
BssAI RCCGGY 2 cut(s) 299, 372
BssECI CCNNGG 3 cut(s) 151, 708, 1154
BssMI GATC 1 cut(s) 360
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 1154
Bst2BI CACGAG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 916, 1057
Bst6I CTCTTC 3 cut(s) 869, 920, 1225
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 271, 301, 417
BstDEI CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 8 cut(s) 23, 120, 216, 240, 544, 873, 1279, 1300
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 3 cut(s) 6, 421, 859
BstKTI GATC 1 cut(s) 363
BstMAI GTCTC 3 cut(s) 253, 790, 816
BstMBI GATC 1 cut(s) 360
BstMWI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 348, 745
BstSLI GKGCMC 1 cut(s) 1224
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 519
BstV2I GAAGAC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 151
BtgZI GCGATG 1 cut(s) 977
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 8 cut(s) 23, 120, 216, 240, 544, 873, 1279, 1300
Cac8I GCNNGC 3 cut(s) 271, 301, 417
CaiI CAGNNNCTG 2 cut(s) 421, 887
CciI TCATGA 1 cut(s) 895
CfoI GCGC 3 cut(s) 6, 421, 859
Cfr10I RCCGGY 2 cut(s) 299, 372
Cfr13I GGNCC 1 cut(s) 211
CseI GACGC 1 cut(s) 449
Csp6I GTAC 4 cut(s) 550, 616, 766, 817
CviAII CATG 4 cut(s) 169, 503, 896, 1040
CviQI GTAC 4 cut(s) 550, 616, 766, 817
DdeI CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
DpnI GATC 1 cut(s) 362
DpnII GATC 1 cut(s) 360
DraIII CACNNNGTG 1 cut(s) 1057
DriI GACNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 3 cut(s) 869, 920, 1225
Eam1105I GACNNNNNGTC 1 cut(s) 844
EarI CTCTTC 3 cut(s) 869, 920, 1225
EciI GGCGGA 2 cut(s) 482, 536
Eco130I CCWWGG 1 cut(s) 1154
Eco31I GGTCTC 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 983
Eco88I CYCGRG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 1154
ErhI CCWWGG 1 cut(s) 1154
FaeI CATG 4 cut(s) 172, 506, 899, 1043
FatI CATG 4 cut(s) 168, 502, 895, 1039
FauI CCCGC 4 cut(s) 260, 278, 458, 1278
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 274, 488, 533
FokI GGATG 8 cut(s) 10, 107, 203, 227, 551, 880, 1286, 1307
Fsp4HI GCNGC 3 cut(s) 274, 488, 533
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 3 cut(s) 606, 1029, 1155
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 420, 858
GluI GCNGC 3 cut(s) 274, 488, 533
GsuI CTGGAG 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 1 cut(s) 299
HapII CCGG 6 cut(s) 300, 350, 373, 536, 747, 831
HgaI GACGC 1 cut(s) 449
HhaI GCGC 3 cut(s) 6, 421, 859
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 4 cut(s) 172, 506, 899, 1043
Hin6I GCGC 3 cut(s) 4, 419, 857
HinP1I GCGC 3 cut(s) 4, 419, 857
HincII GTYRAC 3 cut(s) 114, 378, 912
HindII GTYRAC 3 cut(s) 114, 378, 912
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 4 cut(s) 141, 892, 1036, 1337
HpaII CCGG 6 cut(s) 300, 350, 373, 536, 747, 831
HphI GGTGA 2 cut(s) 79, 439
Hpy166II GTNNAC 3 cut(s) 114, 378, 912
Hpy188I TCNGA 4 cut(s) 510, 774, 1318, 1342
Hpy188III TCNNGA 7 cut(s) 34, 209, 334, 397, 437, 466, 896
Hpy8I GTNNAC 3 cut(s) 114, 378, 912
HpyAV CCTTC 3 cut(s) 1075, 1116, 1153
HpyCH4III ACNGT 2 cut(s) 916, 1057
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 81, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
HpyF3I CTNAG 5 cut(s) 455, 597, 671, 1064, 1134
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 4 cut(s) 172, 506, 899, 1043
HspAI GCGC 3 cut(s) 4, 419, 857
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 1 cut(s) 360
LguI GCTCTTC 1 cut(s) 920
LmnI GCTCC 2 cut(s) 468, 495
Lsp1109I GCAGC 1 cut(s) 519
LweI GCATC 3 cut(s) 583, 694, 1012
MaeI CTAG 3 cut(s) 606, 1029, 1155
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 445, 1187
MalI GATC 1 cut(s) 362
MboI GATC 1 cut(s) 360
MboII GAAGA 6 cut(s) 322, 886, 934, 937, 1217, 1242
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 4 cut(s) 315, 348, 932, 1224
MlyI GAGTC 1 cut(s) 1331
MmeI TCCRAC 1 cut(s) 501
MroNI GCCGGC 1 cut(s) 299
MseI TTAA 2 cut(s) 14, 753
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 6 cut(s) 300, 350, 373, 536, 747, 831
MspR9I CCNGG 2 cut(s) 350, 747
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 1252
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 153, 279, 412, 425
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 2 cut(s) 350, 747
NdeII GATC 1 cut(s) 360
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 4 cut(s) 172, 506, 899, 1043
NlaIV GGNNCC 2 cut(s) 362, 491
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 339
PctI GAATGC 1 cut(s) 1252
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 3 cut(s) 141, 892, 1036
Pfl23II CGTACG 1 cut(s) 816
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 3 cut(s) 275, 489, 534
PleI GAGTC 1 cut(s) 1331
PpsI GAGTC 1 cut(s) 1331
PpuMI RGGWCCY 1 cut(s) 211
PsiI TTATAA 1 cut(s) 1077
Psp5II RGGWCCY 1 cut(s) 211
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 2 cut(s) 362, 491
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PspXI VCTCGAGB 1 cut(s) 497
PstNI CAGNNNCTG 2 cut(s) 421, 887
PsuI RGATCY 1 cut(s) 360
PsyI GACNNNGTC 1 cut(s) 443
RsaI GTAC 4 cut(s) 551, 617, 767, 818
RsaNI GTAC 4 cut(s) 550, 616, 766, 817
SalI GTCGAC 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 2 cut(s) 14, 753
SatI GCNGC 3 cut(s) 274, 488, 533
Sau3AI GATC 1 cut(s) 360
Sau96I GGNCC 1 cut(s) 211
ScaI AGTACT 2 cut(s) 551, 767
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 2 cut(s) 350, 747
SduI GDGCHC 4 cut(s) 315, 348, 932, 1224
SfaNI GCATC 3 cut(s) 583, 694, 1012
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 497, 841
SmoI CTYRAG 2 cut(s) 497, 841
SspMI CTAG 3 cut(s) 606, 1029, 1155
StyD4I CCNGG 2 cut(s) 348, 745
StyI CCWWGG 1 cut(s) 1154
TaaI ACNGT 2 cut(s) 916, 1057
TaiI ACGT 1 cut(s) 330
TaqI TCGA 7 cut(s) 113, 177, 258, 333, 498, 546, 848
TatI WGTACW 3 cut(s) 549, 615, 765
TauI GCSGC 2 cut(s) 276, 490
TfiI GAWTC 3 cut(s) 141, 892, 1036
Tru1I TTAA 2 cut(s) 14, 753
Tru9I TTAA 2 cut(s) 14, 753
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 1 cut(s) 532
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 3 cut(s) 884, 912, 980
TspGWI ACGGA 1 cut(s) 804
Tth111I GACNNNGTC 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 4 cut(s) 203, 317, 736, 967
XceI RCATGY 1 cut(s) 506
XhoI CTCGAG 1 cut(s) 497
XmaJI CCTAGG 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 3 cut(s) 606, 1029, 1155
ZrmI AGTACT 2 cut(s) 551, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.