MD01G1198700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013887
Physical Location & Seq
Reverse (-)
29635013 .. 29636279
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1198700.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGCTCCGACGCCGGTAGAGCCGAATGGCGAGTCCACGTCCGGCGAGTCGTTGCAGCGAGCTCTGCCGACGCCGTTTTTGATCAAGACGTATCAGCTCGTCGACGATCCCTCAATCAACGACGTTATCTCCTGGAACGACGACGGATCTAGCTTCGTCGTCTGGAACCCCACCGTCTTCGCCAGAGATTTGCTCCCCAAATACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTACGGATTTCGAAAGGTTGGGCTGGACCGATGGGAATTCGCGAATGATTGCTTCCAAAGAGGCCAGAAGCGGCTTCTCTGCGAAATACAACGTCGGAGAAGCACGCCTCTGGCACCGCCGGTGGCGTCGGCATGGGCGGTTCCAGCGGCGAAACCGATGATGTCGCCGTCGAATTCCGGCGACGAGCAGGTGATATCGTCGAGCTCGTCGCCGAACGGAGCTCCCTCGAAACTCATGCAGGAGAACGAGAGGCTGAGGAAGGAGAACATGCACCTCACCAAGGAGTTGGCAGAGGTCAAGACCCTATGCAACAGTATCTTCAGCATGGTCTCGAATTACGCGTGCTCCCAATGGGAAAGCGGCTTCCCGGAGCTGAAACCGCTCGATCTGATACCCGGGAAGCGGTTTTCCGTCAAGGGGGAGAAGGAAGAGGAAGAAGAGGCGAGACTGAAGCTTTTCGGGGTGGCGTTAGGGGCCAAGCGCGCGAGAGAGGCCAGCGCCGACGGCGTAGAGGAGGATGAGACCGACTTGCGGCTTCAGCCGAGTGAGCGATTATTGGTAGGAAGTTACGGTAGATATTAG

Protein Analysis

277

Amino Acids

30.97

Weight (kDa)

5.91

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 5.8e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 361
AccBSI CCGCTC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 102
AccII CGCG 4 cut(s) 290, 590, 732, 734
AciI CCGC 9 cut(s) 235, 319, 365, 386, 395, 609, 629, 652, 781
AclWI GGATC 2 cut(s) 101, 154
AcsI RAATTY 2 cut(s) 284, 421
AcuI CTGAAG 3 cut(s) 553, 719, 770
AcyI GRCGYC 3 cut(s) 11, 71, 374
AfiI CCNNNNNNNGG 4 cut(s) 529, 651, 666, 780
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 1 cut(s) 208
AjiI CACGTC 1 cut(s) 39
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 9 cut(s) 62, 97, 153, 228, 241, 453, 470, 622, 703
AluI AGCT 9 cut(s) 62, 97, 153, 228, 241, 453, 470, 622, 703
Alw21I GWGCWC 4 cut(s) 64, 455, 472, 596
Alw26I GTCTC 3 cut(s) 583, 688, 764
AlwI GGATC 2 cut(s) 101, 154
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 3 cut(s) 310, 723, 741
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 2 cut(s) 284, 421
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 61, 93
Asp700I GAANNNNTTC 1 cut(s) 704
AspLEI GCGC 3 cut(s) 732, 734, 749
AspS9I GGNCC 2 cut(s) 274, 723
AsuC2I CCSGG 3 cut(s) 617, 645, 646
AsuHPI GGTGA 2 cut(s) 451, 517
AsuII TTCGAA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 3 cut(s) 64, 455, 472
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 4 cut(s) 64, 455, 472, 596
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 273
BceAI ACGGC 3 cut(s) 58, 400, 769
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 1 cut(s) 81
BcnI CCSGG 3 cut(s) 617, 645, 646
BcoDI GTCTC 3 cut(s) 583, 688, 764
BfaI CTAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 5 cut(s) 56, 320, 396, 610, 782
BlsI GCNGC 5 cut(s) 57, 321, 397, 611, 783
Bme1390I CCNGG 4 cut(s) 133, 617, 645, 646
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 644
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 274, 723
BmiI GGNNCC 5 cut(s) 6, 167, 363, 390, 724
BmrFI CCNGG 4 cut(s) 133, 617, 645, 646
BpiI GAAGAC 1 cut(s) 169
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 503
Bpu14I TTCGAA 1 cut(s) 259
BpuMI CCSGG 3 cut(s) 617, 645, 646
BsaHI GRCGYC 3 cut(s) 11, 71, 374
BsaI GGTCTC 2 cut(s) 583, 764
BsaJI CCNNGG 2 cut(s) 528, 644
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 206, 236, 578, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 529, 651, 666, 780
Bse118I RCCGGY 2 cut(s) 13, 367
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 2 cut(s) 528, 644
BseGI GGATG 1 cut(s) 772
BseLI CCNNNNNNNGG 4 cut(s) 529, 651, 666, 780
BseMII CTCAG 1 cut(s) 494
BsePI GCGCGC 1 cut(s) 730
BseRI GAGGAG 1 cut(s) 776
BseXI GCAGC 1 cut(s) 67
Bsh1236I CGCG 4 cut(s) 290, 590, 732, 734
BshFI GGCC 3 cut(s) 312, 725, 743
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 4 cut(s) 64, 455, 472, 596
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 6 cut(s) 14, 42, 368, 426, 617, 645
BslI CCNNNNNNNGG 4 cut(s) 529, 651, 666, 780
BsmAI GTCTC 3 cut(s) 583, 688, 764
BsnI GGCC 3 cut(s) 312, 725, 743
Bso31I GGTCTC 2 cut(s) 583, 764
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 4 cut(s) 64, 455, 472, 596
Bsp143I GATC 4 cut(s) 81, 106, 146, 634
Bsp68I TCGCGA 1 cut(s) 290
BspACI CCGC 9 cut(s) 235, 319, 365, 386, 395, 609, 629, 652, 781
BspANI GGCC 3 cut(s) 312, 725, 743
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 4 cut(s) 290, 590, 732, 734
BspLI GGNNCC 5 cut(s) 6, 167, 363, 390, 724
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 101, 154
BspT104I TTCGAA 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 583, 764
BsrBI CCGCTC 1 cut(s) 631
BsrFI RCCGGY 2 cut(s) 13, 367
BssAI RCCGGY 2 cut(s) 13, 367
BssECI CCNNGG 2 cut(s) 528, 644
BssHII GCGCGC 1 cut(s) 730
BssMI GATC 4 cut(s) 81, 106, 146, 634
BssNI GRCGYC 3 cut(s) 11, 71, 374
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 3 cut(s) 175, 563, 821
Bst6I CTCTTC 2 cut(s) 672, 681
BstACI GRCGYC 3 cut(s) 11, 71, 374
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 6 cut(s) 60, 239, 353, 592, 732, 745
BstDEI CTNAG 1 cut(s) 503
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 1 cut(s) 772
BstFNI CGCG 4 cut(s) 290, 590, 732, 734
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 3 cut(s) 732, 734, 749
BstKTI GATC 4 cut(s) 84, 109, 149, 637
BstMAI GTCTC 3 cut(s) 583, 688, 764
BstMBI GATC 4 cut(s) 81, 106, 146, 634
BstNI CCWGG 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 4 cut(s) 131, 615, 643, 644
BstUI CGCG 4 cut(s) 290, 590, 732, 734
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 3 cut(s) 312, 725, 743
BtrI CACGTC 1 cut(s) 39
BtsCI GGATG 1 cut(s) 772
BtuMI TCGCGA 1 cut(s) 290
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 6 cut(s) 60, 239, 353, 592, 732, 745
CfoI GCGC 3 cut(s) 732, 734, 749
Cfr10I RCCGGY 2 cut(s) 13, 367
Cfr13I GGNCC 2 cut(s) 274, 723
Cfr9I CCCGGG 1 cut(s) 644
CseI GACGC 3 cut(s) 19, 79, 363
CviAII CATG 4 cut(s) 381, 484, 517, 574
DdeI CTNAG 1 cut(s) 503
DpnI GATC 4 cut(s) 83, 108, 148, 636
DpnII GATC 4 cut(s) 81, 106, 146, 634
Eam1104I CTCTTC 2 cut(s) 672, 681
EarI CTCTTC 2 cut(s) 672, 681
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 3 cut(s) 62, 453, 470
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 3 cut(s) 64, 455, 472
Eco31I GGTCTC 2 cut(s) 583, 764
Eco32I GATATC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 3 cut(s) 62, 453, 470
Eco57I CTGAAG 3 cut(s) 553, 719, 770
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 3 cut(s) 62, 453, 470
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRI GAATTC 2 cut(s) 284, 421
EcoRII CCWGG 1 cut(s) 131
EcoRV GATATC 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 3 cut(s) 64, 455, 472
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 4 cut(s) 384, 487, 520, 577
FaiI YATR 5 cut(s) 382, 485, 518, 556, 575
FatI CATG 4 cut(s) 380, 483, 516, 573
FbaI TGATCA 1 cut(s) 81
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 56, 320, 396, 610, 782
FokI GGATG 1 cut(s) 779
FriOI GRGCYC 3 cut(s) 64, 455, 472
Fsp4HI GCNGC 5 cut(s) 56, 320, 396, 610, 782
FspBI CTAG 1 cut(s) 150
GlaI GCGC 3 cut(s) 731, 733, 748
GluI GCNGC 5 cut(s) 56, 320, 396, 610, 782
GsuI CTGGAG 1 cut(s) 208
HaeII RGCGCY 1 cut(s) 750
HaeIII GGCC 3 cut(s) 312, 725, 743
HapII CCGG 6 cut(s) 14, 42, 368, 426, 617, 645
HgaI GACGC 3 cut(s) 19, 79, 363
HhaI GCGC 3 cut(s) 732, 734, 749
Hin1I GRCGYC 3 cut(s) 11, 71, 374
Hin1II CATG 4 cut(s) 384, 487, 520, 577
Hin6I GCGC 3 cut(s) 730, 732, 747
HinP1I GCGC 3 cut(s) 730, 732, 747
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 2 cut(s) 32, 47
HpaII CCGG 6 cut(s) 14, 42, 368, 426, 617, 645
HphI GGTGA 2 cut(s) 451, 517
Hpy166II GTNNAC 2 cut(s) 36, 103
Hpy188I TCNGA 3 cut(s) 9, 345, 639
Hpy188III TCNNGA 5 cut(s) 85, 163, 289, 547, 580
Hpy8I GTNNAC 2 cut(s) 36, 103
HpyAV CCTTC 2 cut(s) 502, 667
HpyCH4III ACNGT 3 cut(s) 175, 563, 821
HpyCH4IV ACGT 4 cut(s) 38, 89, 123, 340
HpyCH4V TGCA 4 cut(s) 55, 487, 520, 558
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 4 cut(s) 38, 89, 123, 340
Hsp92I GRCGYC 3 cut(s) 11, 71, 374
Hsp92II CATG 4 cut(s) 384, 487, 520, 577
HspAI GCGC 3 cut(s) 730, 732, 747
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 4 cut(s) 81, 106, 146, 634
LmnI GCTCC 6 cut(s) 10, 198, 467, 475, 599, 619
Lsp1109I GCAGC 1 cut(s) 67
MaeI CTAG 1 cut(s) 150
MaeII ACGT 4 cut(s) 38, 89, 123, 340
MaeIII GTNAC 1 cut(s) 815
MalI GATC 4 cut(s) 83, 108, 148, 636
MbiI CCGCTC 1 cut(s) 631
MboI GATC 4 cut(s) 81, 106, 146, 634
MboII GAAGA 5 cut(s) 169, 559, 689, 695, 698
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 4 cut(s) 64, 455, 472, 596
MluCI AATT 3 cut(s) 284, 421, 583
MluI ACGCGT 1 cut(s) 588
MlyI GAGTC 2 cut(s) 41, 56
MmeI TCCRAC 2 cut(s) 32, 323
MroXI GAANNNNTTC 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 6 cut(s) 14, 42, 368, 426, 617, 645
MspR9I CCNGG 4 cut(s) 133, 617, 645, 646
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 4 cut(s) 290, 590, 732, 734
NciI CCSGG 3 cut(s) 617, 645, 646
NdeII GATC 4 cut(s) 81, 106, 146, 634
NlaIII CATG 4 cut(s) 384, 487, 520, 577
NlaIV GGNNCC 5 cut(s) 6, 167, 363, 390, 724
NmeAIII GCCGAG 1 cut(s) 816
NruI TCGCGA 1 cut(s) 290
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 259
PaqCI CACCTGC 1 cut(s) 427
PauI GCGCGC 1 cut(s) 730
PcsI WCGNNNNNNNCGW 2 cut(s) 452, 587
PdmI GAANNNNTTC 1 cut(s) 704
PfoI TCCNGGA 2 cut(s) 131, 615
PkrI GCNGC 5 cut(s) 57, 321, 397, 611, 783
PleI GAGTC 2 cut(s) 40, 55
PpsI GAGTC 2 cut(s) 40, 55
Psp124BI GAGCTC 3 cut(s) 64, 455, 472
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 5 cut(s) 6, 167, 363, 390, 724
PspPI GGNCC 2 cut(s) 274, 723
PsuI RGATCY 1 cut(s) 146
PteI GCGCGC 1 cut(s) 730
RruI TCGCGA 1 cut(s) 290
SacI GAGCTC 3 cut(s) 64, 455, 472
SalI GTCGAC 1 cut(s) 101
SatI GCNGC 5 cut(s) 56, 320, 396, 610, 782
Sau3AI GATC 4 cut(s) 81, 106, 146, 634
Sau96I GGNCC 2 cut(s) 274, 723
SchI GAGTC 2 cut(s) 41, 56
ScrFI CCNGG 4 cut(s) 133, 617, 645, 646
SduI GDGCHC 4 cut(s) 64, 455, 472, 596
SfuI TTCGAA 1 cut(s) 259
SgrAI CRCCGGYG 1 cut(s) 367
SgrDI CGTCGACG 1 cut(s) 101
SinI GGWCC 1 cut(s) 274
SmaI CCCGGG 1 cut(s) 646
Sse9I AATT 3 cut(s) 284, 421, 583
SsiI CCGC 9 cut(s) 235, 319, 365, 386, 395, 609, 629, 652, 781
SspMI CTAG 1 cut(s) 150
SstI GAGCTC 3 cut(s) 64, 455, 472
StyD4I CCNGG 4 cut(s) 131, 615, 643, 644
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 3 cut(s) 175, 563, 821
TaiI ACGT 4 cut(s) 41, 92, 126, 343
TaqI TCGA 7 cut(s) 102, 259, 419, 449, 476, 581, 633
TaqII GACCGA 2 cut(s) 291, 788
TasI AATT 3 cut(s) 284, 421, 583
TauI GCSGC 4 cut(s) 322, 398, 612, 784
TseI GCWGC 1 cut(s) 55
TspGWI ACGGA 4 cut(s) 159, 267, 480, 649
TspMI CCCGGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 274
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 2 cut(s) 284, 421
XceI RCATGY 1 cut(s) 520
XmaI CCCGGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 704
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.