Rorug01G0399900

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013887
Physical Location & Seq
Reverse (-)
50832128 .. 50834441
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0399900.1

Sequence Viewer

Length: 1167 bp
ATGGGTGCTTATGATCAAGGTTCTAATGGGTTTTCCCAGCCCTTGGATAGTTCTAAGAAAAGGGGCCGTCGAAGTAAAAAGGATGGAGCCAGTGTTGAGGAGACCCTGTCAAGGTGGAAAGAATATAACAAGCTTTTAGAGTCTGGGATTTACGATGACAAGGGGCGTAAGATTCAGGCCAAGGGGTCGAAGAAGGGTTGTATGAAAGGTAAAGGAGGGCCGGAGAATCAGCGGTGTAATTACAGAGGTGTTAGGCAGAGGACGTGGGGAAAGTGGGTTGCAGAAATCCGAGAGCCCAACAGAGGAAGTAGGCTTTGGTTAGGTACTTTTCCCACGGCTGTTGATGCTGCACTTGCTTATGATGAAGCTGCAAAAGCCATGTATGGTGCCACTGCTCGCCTTAACCACCCTAATGTAAACAATCACAACAGTTATACATCTTCTTGGAATGAATCGTCACATGAAACTTCGTCAGTCACAACTCCATCAGGGTGTTCTACTGTGGTAACACAAGGATGCTGTGAATCTATTACGATATCAAATCGGTCTGATCACTCCGAGGTCGTCTGTGCAGAGGAGGAATCTTTTATAGTGAAAACTGAGGACGGTGAGGATGAATCGACAAGTCATGCTTGGCCTAATGCATTTACTCAACCCAGTGCGTCTAATGCTACGGCAGATGTAGAGGTGAAAAGTGAGGTTCCTATGGACCACTTCCACACAGATCAACCTGGAGTCCAAGAGCAGGCACATGACTGGATTAGCACAGATGGCTTTGATATGGATTGCTTGAAGCAGTTTTCAATTGATGAGCTTTTTTATGTGGACGAGCTTGTCGGCCCTATGGATGGTAAACCCATCCCTGCTCCGAGATTGCAGCAAGATTTAAGCTCTGATGTTGGTCGTCCTCAGTTCACGGATATGGTCCCTGTTGATAATGCCACACTATCGGATTCATCATGCCAGTTACAGTATCCAGATAACAAGCCTCCTGGTGGTAATGTGCAGCATATGGACGTGGAGCCATCAGTTGCCGACTATGGCTTTGATTTGTTAAATCGTCAGATGCAAGAGAATAATGTTGGAGCAGATAATCACGGATACTTCAATGTAGATGATTTCGATTTCAATTTCACTGGAGGCGGCGGCGGCGGCAATGCAAACTGA

Protein Analysis

388

Amino Acids

42.62

Weight (kDa)

4.85

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 80 - 129 2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 43
AciI CCGC 5 cut(s) 232, 1143, 1146, 1149, 1152
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 6 cut(s) 43, 111, 302, 745, 848, 995
AgsI TTSAA 4 cut(s) 793, 804, 1108, 1129
AjiI CACGTC 2 cut(s) 264, 1018
AjnI CCWGG 2 cut(s) 730, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AluBI AGCT 5 cut(s) 133, 368, 814, 832, 891
AluI AGCT 5 cut(s) 133, 368, 814, 832, 891
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 5 cut(s) 64, 177, 218, 635, 838
ApeKI GCWGC 4 cut(s) 347, 368, 877, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
AspS9I GGNCC 5 cut(s) 64, 218, 709, 839, 925
AsuHPI GGTGA 2 cut(s) 620, 700
AvaII GGWCC 2 cut(s) 709, 925
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 297
BbvI GCAGC 4 cut(s) 334, 355, 889, 1018
BccI CCATC 6 cut(s) 77, 493, 764, 842, 866, 1033
BceAI ACGGC 3 cut(s) 51, 351, 690
BciT130I CCWGG 2 cut(s) 732, 993
BciVI GTATCC 2 cut(s) 984, 1094
BclI TGATCA 2 cut(s) 13, 550
BcoDI GTCTC 1 cut(s) 95
BfuI GTATCC 2 cut(s) 984, 1094
BisI GCNGC 8 cut(s) 348, 369, 878, 1007, 1144, 1147, 1150, 1153
BlsI GCNGC 8 cut(s) 349, 370, 879, 1008, 1145, 1148, 1151, 1154
Bme1390I CCNGG 2 cut(s) 732, 993
Bme18I GGWCC 2 cut(s) 709, 925
BmgBI CACGTC 2 cut(s) 264, 1018
BmgT120I GGNCC 5 cut(s) 64, 218, 709, 839, 925
BmiI GGNNCC 6 cut(s) 65, 88, 388, 702, 927, 1023
BmrFI CCNGG 2 cut(s) 732, 993
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 3 cut(s) 334, 506, 1056
BmuI ACTGGG 1 cut(s) 651
BpmI CTGGAG 2 cut(s) 753, 1158
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 4 cut(s) 42, 180, 333, 558
BsaXI ACNNNNNCTCC 4 cut(s) 78, 92, 108, 122
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 111, 302, 745, 848, 995
Bse1I ACTGG 5 cut(s) 90, 657, 761, 964, 1141
Bse3DI GCAATG 1 cut(s) 1162
BseBI CCWGG 2 cut(s) 732, 993
BseDI CCNNGG 4 cut(s) 42, 180, 333, 558
BseGI GGATG 5 cut(s) 88, 521, 619, 853, 858
BseLI CCNNNNNNNGG 6 cut(s) 43, 111, 302, 745, 848, 995
BseMI GCAATG 1 cut(s) 1162
BseMII CTCAG 2 cut(s) 591, 923
BseNI ACTGG 5 cut(s) 90, 657, 761, 964, 1141
BseRI GAGGAG 2 cut(s) 113, 590
BseXI GCAGC 4 cut(s) 334, 355, 889, 1018
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 3 cut(s) 333, 591, 1025
BshFI GGCC 5 cut(s) 66, 179, 220, 637, 840
BshNI GGYRCC 1 cut(s) 386
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 6 cut(s) 43, 111, 302, 745, 848, 995
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 911
BsnI GGCC 5 cut(s) 66, 179, 220, 637, 840
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 3 cut(s) 13, 550, 724
BspACI CCGC 5 cut(s) 232, 1143, 1146, 1149, 1152
BspANI GGCC 5 cut(s) 66, 179, 220, 637, 840
BspCNI CTCAG 2 cut(s) 592, 922
BspLI GGNNCC 6 cut(s) 65, 88, 388, 702, 927, 1023
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 1162
BsrI ACTGG 5 cut(s) 90, 657, 761, 964, 1141
BssECI CCNNGG 4 cut(s) 42, 180, 333, 558
BssMI GATC 3 cut(s) 13, 550, 724
BssT1I CCWWGG 2 cut(s) 42, 180
Bst2UI CCWGG 2 cut(s) 732, 993
Bst4CI ACNGT 4 cut(s) 431, 502, 608, 972
BstC8I GCNNGC 2 cut(s) 397, 747
BstDEI CTNAG 3 cut(s) 54, 600, 909
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 5 cut(s) 88, 521, 619, 853, 858
BstKTI GATC 3 cut(s) 16, 553, 727
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 13, 550, 724
BstMWI GCNNNNNNNGC 7 cut(s) 344, 353, 374, 668, 771, 1149, 1152
BstNI CCWGG 2 cut(s) 732, 993
BstSCI CCNGG 2 cut(s) 730, 991
BstV1I GCAGC 4 cut(s) 334, 355, 889, 1018
BsuI GTATCC 2 cut(s) 984, 1094
BsuRI GGCC 5 cut(s) 66, 179, 220, 637, 840
BtgI CCRYGG 1 cut(s) 333
BtrI CACGTC 2 cut(s) 264, 1018
BtsCI GGATG 5 cut(s) 88, 521, 619, 853, 858
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 4 cut(s) 97, 390, 664, 1134
Cac8I GCNNGC 2 cut(s) 397, 747
Cfr13I GGNCC 5 cut(s) 64, 218, 709, 839, 925
CseI GACGC 1 cut(s) 651
Csp6I GTAC 1 cut(s) 324
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 5 cut(s) 379, 461, 629, 752, 960
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 3 cut(s) 54, 600, 909
DpnI GATC 3 cut(s) 15, 552, 726
DpnII GATC 3 cut(s) 13, 550, 724
DrdI GACNNNNNNGTC 1 cut(s) 833
DseDI GACNNNNNNGTC 1 cut(s) 833
Eco130I CCWWGG 2 cut(s) 42, 180
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 95
Eco32I GATATC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 709, 925
EcoRII CCWGG 2 cut(s) 730, 991
EcoRV GATATC 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 42, 180
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 2 cut(s) 42, 180
FaeI CATG 5 cut(s) 382, 464, 632, 755, 963
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FaqI GGGAC 1 cut(s) 911
FatI CATG 5 cut(s) 378, 460, 628, 751, 959
FauNDI CATATG 1 cut(s) 1011
FbaI TGATCA 2 cut(s) 13, 550
Fnu4HI GCNGC 8 cut(s) 348, 369, 878, 1007, 1144, 1147, 1150, 1153
FokI GGATG 5 cut(s) 95, 528, 626, 845, 860
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 8 cut(s) 348, 369, 878, 1007, 1144, 1147, 1150, 1153
GluI GCNGC 8 cut(s) 348, 369, 878, 1007, 1144, 1147, 1150, 1153
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 2 cut(s) 753, 1158
HaeIII GGCC 5 cut(s) 66, 179, 220, 637, 840
HapII CCGG 1 cut(s) 221
HgaI GACGC 1 cut(s) 651
Hin1II CATG 5 cut(s) 382, 464, 632, 755, 963
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 9 cut(s) 140, 172, 226, 452, 524, 581, 617, 735, 953
HpaII CCGG 1 cut(s) 221
HphI GGTGA 2 cut(s) 620, 700
Hpy166II GTNNAC 4 cut(s) 418, 826, 854, 915
Hpy188I TCNGA 7 cut(s) 290, 550, 559, 870, 895, 952, 1065
Hpy188III TCNNGA 1 cut(s) 977
Hpy8I GTNNAC 4 cut(s) 418, 826, 854, 915
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 4 cut(s) 431, 502, 608, 972
HpyCH4IV ACGT 2 cut(s) 263, 1017
HpyCH4V TGCA 9 cut(s) 281, 350, 371, 572, 644, 877, 1006, 1069, 1160
HpyF10VI GCNNNNNNNGC 7 cut(s) 344, 353, 374, 668, 771, 1149, 1152
HpyF3I CTNAG 3 cut(s) 54, 600, 909
HpySE526I ACGT 2 cut(s) 263, 1017
Hsp92II CATG 5 cut(s) 382, 464, 632, 755, 963
Ksp22I TGATCA 2 cut(s) 13, 550
Kzo9I GATC 3 cut(s) 13, 550, 724
LmnI GCTCC 4 cut(s) 86, 871, 1021, 1085
Lsp1109I GCAGC 4 cut(s) 334, 355, 889, 1018
LweI GCATC 3 cut(s) 334, 506, 1056
MaeII ACGT 2 cut(s) 263, 1017
MaeIII GTNAC 4 cut(s) 456, 475, 505, 966
MalI GATC 3 cut(s) 15, 552, 726
MboI GATC 3 cut(s) 13, 550, 724
MboII GAAGA 2 cut(s) 202, 432
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 3 cut(s) 238, 804, 1129
MlyI GAGTC 2 cut(s) 149, 744
MmeI TCCRAC 1 cut(s) 1063
Mph1103I ATGCAT 1 cut(s) 646
MseI TTAA 3 cut(s) 402, 887, 1055
MslI CAYNNNNRTG 4 cut(s) 411, 490, 514, 920
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 732, 993
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 2 cut(s) 732, 993
MwoI GCNNNNNNNGC 7 cut(s) 344, 353, 374, 668, 771, 1149, 1152
NdeI CATATG 1 cut(s) 1011
NdeII GATC 3 cut(s) 13, 550, 724
NlaIII CATG 5 cut(s) 382, 464, 632, 755, 963
NlaIV GGNNCC 6 cut(s) 65, 88, 388, 702, 927, 1023
NmuCI GTSAC 2 cut(s) 456, 475
NsiI ATGCAT 1 cut(s) 646
PfeI GAWTC 7 cut(s) 172, 226, 452, 524, 581, 617, 953
PflFI GACNNNGTC 1 cut(s) 106
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 8 cut(s) 349, 370, 879, 1008, 1145, 1148, 1151, 1154
PleI GAGTC 2 cut(s) 148, 743
PpsI GAGTC 2 cut(s) 148, 743
Psp6I CCWGG 2 cut(s) 730, 991
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 2 cut(s) 730, 991
PspN4I GGNNCC 6 cut(s) 65, 88, 388, 702, 927, 1023
PspPI GGNCC 5 cut(s) 64, 218, 709, 839, 925
PsyI GACNNNGTC 1 cut(s) 106
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
RseI CAYNNNNRTG 4 cut(s) 411, 490, 514, 920
SaqAI TTAA 3 cut(s) 402, 887, 1055
SatI GCNGC 8 cut(s) 348, 369, 878, 1007, 1144, 1147, 1150, 1153
Sau3AI GATC 3 cut(s) 13, 550, 724
Sau96I GGNCC 5 cut(s) 64, 218, 709, 839, 925
SchI GAGTC 2 cut(s) 149, 744
ScrFI CCNGG 2 cut(s) 732, 993
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 3 cut(s) 334, 506, 1056
SinI GGWCC 2 cut(s) 709, 925
SmiMI CAYNNNNRTG 4 cut(s) 411, 490, 514, 920
Sse9I AATT 3 cut(s) 238, 804, 1129
SsiI CCGC 5 cut(s) 232, 1143, 1146, 1149, 1152
StyD4I CCNGG 2 cut(s) 730, 991
StyI CCWWGG 2 cut(s) 42, 180
TaaI ACNGT 4 cut(s) 431, 502, 608, 972
TaiI ACGT 2 cut(s) 266, 1020
TaqI TCGA 4 cut(s) 70, 188, 620, 1122
TaqII GACCGA 1 cut(s) 534
TasI AATT 3 cut(s) 238, 804, 1129
TauI GCSGC 4 cut(s) 1146, 1149, 1152, 1155
TfiI GAWTC 7 cut(s) 172, 226, 452, 524, 581, 617, 953
Tru1I TTAA 3 cut(s) 402, 887, 1055
Tru9I TTAA 3 cut(s) 402, 887, 1055
TscAI CASTG 4 cut(s) 97, 397, 664, 1141
TseFI GTSAC 2 cut(s) 456, 475
TseI GCWGC 4 cut(s) 347, 368, 877, 1006
Tsp45I GTSAC 2 cut(s) 456, 475
TspDTI ATGAA 6 cut(s) 218, 378, 465, 477, 630, 945
TspGWI ACGGA 2 cut(s) 932, 1113
TspRI CASTG 4 cut(s) 97, 397, 664, 1141
Tth111I GACNNNGTC 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 709, 925
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.