MD07G1266500.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013887
Physical Location & Seq
Reverse (-)
33486432 .. 33488085
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1266500.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGCTCCGACGCCGGTGGAGCCGACCGGCGAGTCCACGTCCAGCGAGTCGTCGCAGCGAGCTCTTCCGACTCCGTTTTTGACCAAGACGTATCAGCTCGTCGACGATCCCTCAATCGACGACGTCATCTCATGGAGCGACGACGGATCCAGCTTCGTCGTCTGGAACCCCACCGTCTTCGCCAGAGATTTGCTCCCCAAGTACTTCAAGCACAACAACTTCTCCAGCTTCGTACGGCAGCTCAACACCTACGGATTTCGGAAGGTTGTGCCGGACCGATGGGAATTCTTGAACGATAGCTTTCGGAGAGGCGAGAAGCGGCTTCTCTGCGAAATACAACGCCGGAAAATCAACCCTCCGGCGCCGCCGGTGGCGTCGGTTTGGGCGGTTCCGACGGCAAAACCGATTATTTCCCCGGCGAACTCGGGCGACGAGCAGGTGATATCTTCGAGTTCGTCGCCGATCAGAGCATCGTCGGAGATTATGGACGAGAACGAGAGGCTGAGGAAAGAGAACATGCAGCTCACCAAGGAGCTGGTGGAGCTGAAGTCCCTCTGCAACAACATCTTCACCATGGTATCGAATTACGCTTGCGCCCAATCGGAGAGCGGTTTCCCGGAGCTAAAACCGCTCGATTTGATGCCCGGGAAGCGTTTCCCCGGAAAGAGGGAGCACGAAGAGGAAGAAGAGGCGAGTCCGAAACTTTTCGGGGTGGCGATTGGGGCCAAGCGCGCGCGAGAGGCCCGCGGCGATGGCGTAGAGAACGATGAGACCAACTTGCGGCTTCAGCAACCGAGCGCTAGCGAGGTTAAATCAGAGCCGTCGGATATTGGTCATAAGGAAACGCCGTGGCTGAAGCAGTGCCACATGGCTAATCAGAGGGTGTGCAATTGA

Protein Analysis

298

Amino Acids

33.41

Weight (kDa)

5.76

Isoelectric Point (pI)

63.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 7.8e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
AasI GACNNNNNNGTC 1 cut(s) 31
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 361
AccBSI CCGCTC 2 cut(s) 609, 631
AccI GTMKAC 1 cut(s) 102
AccII CGCG 4 cut(s) 732, 734, 736, 747
AciI CCGC 8 cut(s) 319, 365, 386, 609, 629, 745, 747, 781
AclWI GGATC 3 cut(s) 101, 141, 154
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 3 cut(s) 566, 770, 875
AcyI GRCGYC 4 cut(s) 11, 123, 362, 374
AfaI GTAC 2 cut(s) 203, 234
AfeI AGCGCT 1 cut(s) 799
AfiI CCNNNNNNNGG 2 cut(s) 666, 780
AgsI TTSAA 2 cut(s) 208, 292
AjiI CACGTC 1 cut(s) 39
Alw21I GWGCWC 2 cut(s) 64, 675
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 3 cut(s) 101, 141, 154
Ama87I CYCGRG 2 cut(s) 424, 644
Aor51HI AGCGCT 1 cut(s) 799
AoxI GGCC 2 cut(s) 723, 741
ApeKI GCWGC 3 cut(s) 55, 238, 520
ApoI RAATTY 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
Asp700I GAANNNNTTC 2 cut(s) 653, 704
AspLEI GCGC 6 cut(s) 364, 596, 732, 734, 736, 800
AspS9I GGNCC 3 cut(s) 274, 723, 742
AsuC2I CCSGG 5 cut(s) 416, 617, 645, 646, 660
AsuHPI GGTGA 3 cut(s) 451, 517, 562
AsuNHI GCTAGC 1 cut(s) 800
AvaI CYCGRG 2 cut(s) 424, 644
AvaII GGWCC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 146
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 64, 675
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 3 cut(s) 67, 250, 532
BccI CCATC 2 cut(s) 273, 746
BceAI ACGGC 4 cut(s) 251, 411, 805, 832
BcnI CCSGG 5 cut(s) 416, 617, 645, 646, 660
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 1 cut(s) 801
BfoI RGCGCY 2 cut(s) 365, 801
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 7 cut(s) 56, 239, 320, 365, 521, 748, 782
BlsI GCNGC 7 cut(s) 57, 240, 321, 366, 522, 749, 783
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 5 cut(s) 416, 617, 645, 646, 660
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 2 cut(s) 424, 644
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 3 cut(s) 274, 723, 742
BmiI GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
BmrFI CCNGG 5 cut(s) 416, 617, 645, 646, 660
BmsI GCATC 2 cut(s) 479, 630
BmtI GCTAGC 1 cut(s) 804
BpiI GAAGAC 1 cut(s) 169
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 503
BpuMI CCSGG 5 cut(s) 416, 617, 645, 646, 660
BsaHI GRCGYC 4 cut(s) 11, 123, 362, 374
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 7 cut(s) 414, 528, 573, 644, 658, 745, 848
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 533, 563
Bsc4I CCNNNNNNNGG 2 cut(s) 666, 780
Bse118I RCCGGY 3 cut(s) 13, 26, 367
BseDI CCNNGG 7 cut(s) 414, 528, 573, 644, 658, 745, 848
BseLI CCNNNNNNNGG 2 cut(s) 666, 780
BseMII CTCAG 1 cut(s) 494
BsePI GCGCGC 2 cut(s) 730, 732
BseXI GCAGC 3 cut(s) 67, 250, 532
Bsh1236I CGCG 4 cut(s) 732, 734, 736, 747
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 725, 743
BshNI GGYRCC 1 cut(s) 361
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 64, 675
BsiHKCI CYCGRG 2 cut(s) 424, 644
BsiWI CGTACG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 2 cut(s) 666, 780
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 2 cut(s) 725, 743
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 2 cut(s) 424, 644
Bsp1286I GDGCHC 2 cut(s) 64, 675
Bsp143I GATC 3 cut(s) 106, 146, 462
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 8 cut(s) 319, 365, 386, 609, 629, 745, 747, 781
BspANI GGCC 2 cut(s) 725, 743
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 4 cut(s) 732, 734, 736, 747
BspLI GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
BspMI ACCTGC 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 804
BspPI GGATC 3 cut(s) 101, 141, 154
BspQI GCTCTTC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 764
BsrBI CCGCTC 2 cut(s) 609, 631
BsrFI RCCGGY 3 cut(s) 13, 26, 367
BssAI RCCGGY 3 cut(s) 13, 26, 367
BssECI CCNNGG 7 cut(s) 414, 528, 573, 644, 658, 745, 848
BssHII GCGCGC 2 cut(s) 730, 732
BssMI GATC 3 cut(s) 106, 146, 462
BssNI GRCGYC 4 cut(s) 11, 123, 362, 374
BssT1I CCWWGG 2 cut(s) 528, 573
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 3 cut(s) 69, 672, 681
BstACI GRCGYC 4 cut(s) 11, 123, 362, 374
BstC8I GCNNGC 6 cut(s) 60, 592, 732, 734, 745, 802
BstDEI CTNAG 1 cut(s) 503
BstDSI CCRYGG 3 cut(s) 573, 745, 848
BstFNI CGCG 4 cut(s) 732, 734, 736, 747
BstH2I RGCGCY 2 cut(s) 365, 801
BstHHI GCGC 6 cut(s) 364, 596, 732, 734, 736, 800
BstKTI GATC 3 cut(s) 109, 149, 465
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 3 cut(s) 106, 146, 462
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 9 cut(s) 19, 541, 628, 649, 722, 731, 740, 753, 787
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 5 cut(s) 414, 615, 643, 644, 658
BstUI CGCG 4 cut(s) 732, 734, 736, 747
BstV1I GCAGC 3 cut(s) 67, 250, 532
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 725, 743
BtgI CCRYGG 3 cut(s) 573, 745, 848
BtgZI GCGATG 1 cut(s) 765
BtrI CACGTC 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 866
BtsIMutI CAGTG 1 cut(s) 866
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 6 cut(s) 60, 592, 732, 734, 745, 802
CfoI GCGC 6 cut(s) 364, 596, 732, 734, 736, 800
Cfr10I RCCGGY 3 cut(s) 13, 26, 367
Cfr13I GGNCC 3 cut(s) 274, 723, 742
Cfr42I CCGCGG 1 cut(s) 748
Cfr9I CCCGGG 1 cut(s) 644
CpoI CGGWCCG 1 cut(s) 274
CseI GACGC 2 cut(s) 19, 363
Csp6I GTAC 2 cut(s) 202, 233
CspI CGGWCCG 1 cut(s) 274
CviAII CATG 4 cut(s) 132, 517, 574, 868
CviQI GTAC 2 cut(s) 202, 233
DdeI CTNAG 1 cut(s) 503
DinI GGCGCC 1 cut(s) 363
DpnI GATC 3 cut(s) 108, 148, 464
DpnII GATC 3 cut(s) 106, 146, 462
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 69, 672, 681
EarI CTCTTC 3 cut(s) 69, 672, 681
Ecl136II GAGCTC 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 528, 573
Eco24I GRGCYC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 799
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 3 cut(s) 566, 770, 875
Eco88I CYCGRG 2 cut(s) 424, 644
EcoICRI GAGCTC 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 284
EcoRV GATATC 1 cut(s) 444
EcoT14I CCWWGG 2 cut(s) 528, 573
EcoT38I GRGCYC 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 363
EheI GGCGCC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 528, 573
FaeI CATG 4 cut(s) 135, 520, 577, 871
FaiI YATR 6 cut(s) 133, 485, 518, 575, 837, 869
FaqI GGGAC 1 cut(s) 535
FatI CATG 4 cut(s) 131, 516, 573, 867
FauI CCCGC 1 cut(s) 752
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 7 cut(s) 56, 239, 320, 365, 521, 748, 782
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 7 cut(s) 56, 239, 320, 365, 521, 748, 782
FspBI CTAG 1 cut(s) 801
GlaI GCGC 6 cut(s) 363, 595, 731, 733, 735, 799
GluI GCNGC 7 cut(s) 56, 239, 320, 365, 521, 748, 782
GsuI CTGGAG 1 cut(s) 208
HaeII RGCGCY 2 cut(s) 365, 801
HaeIII GGCC 2 cut(s) 725, 743
HgaI GACGC 2 cut(s) 19, 363
HhaI GCGC 6 cut(s) 364, 596, 732, 734, 736, 800
Hin1I GRCGYC 4 cut(s) 11, 123, 362, 374
Hin1II CATG 4 cut(s) 135, 520, 577, 871
Hin6I GCGC 6 cut(s) 362, 594, 730, 732, 734, 798
HinP1I GCGC 6 cut(s) 362, 594, 730, 732, 734, 798
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 4 cut(s) 32, 47, 70, 694
HphI GGTGA 3 cut(s) 451, 517, 562
Hpy166II GTNNAC 2 cut(s) 36, 103
Hpy188III TCNNGA 2 cut(s) 163, 289
Hpy8I GTNNAC 2 cut(s) 36, 103
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 3 cut(s) 38, 89, 123
HpyCH4V TGCA 3 cut(s) 520, 558, 888
HpyF10VI GCNNNNNNNGC 9 cut(s) 19, 541, 628, 649, 722, 731, 740, 753, 787
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 3 cut(s) 38, 89, 123
Hsp92I GRCGYC 4 cut(s) 11, 123, 362, 374
Hsp92II CATG 4 cut(s) 135, 520, 577, 871
HspAI GCGC 6 cut(s) 362, 594, 730, 732, 734, 798
KasI GGCGCC 1 cut(s) 361
KspI CCGCGG 1 cut(s) 748
Kzo9I GATC 3 cut(s) 106, 146, 462
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 8 cut(s) 10, 19, 135, 198, 532, 541, 619, 670
Lsp1109I GCAGC 3 cut(s) 67, 250, 532
LweI GCATC 2 cut(s) 479, 630
MaeI CTAG 1 cut(s) 801
MaeII ACGT 3 cut(s) 38, 89, 123
MalI GATC 3 cut(s) 108, 148, 464
MauBI CGCGCGCG 1 cut(s) 732
MbiI CCGCTC 2 cut(s) 609, 631
MboI GATC 3 cut(s) 106, 146, 462
MboII GAAGA 7 cut(s) 56, 169, 438, 559, 689, 695, 698
MfeI CAATTG 1 cut(s) 889
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 64, 675
MluCI AATT 3 cut(s) 284, 583, 889
Mly113I GGCGCC 1 cut(s) 362
MlyI GAGTC 4 cut(s) 41, 56, 64, 703
MmeI TCCRAC 5 cut(s) 32, 92, 416, 456, 804
MroXI GAANNNNTTC 2 cut(s) 653, 704
MseI TTAA 1 cut(s) 810
MspA1I CMGCKG 1 cut(s) 747
MspR9I CCNGG 5 cut(s) 416, 617, 645, 646, 660
MunI CAATTG 1 cut(s) 889
MvnI CGCG 4 cut(s) 732, 734, 736, 747
MwoI GCNNNNNNNGC 9 cut(s) 19, 541, 628, 649, 722, 731, 740, 753, 787
NarI GGCGCC 1 cut(s) 362
NciI CCSGG 5 cut(s) 416, 617, 645, 646, 660
NcoI CCATGG 1 cut(s) 573
NdeII GATC 3 cut(s) 106, 146, 462
NheI GCTAGC 1 cut(s) 800
NlaIII CATG 4 cut(s) 135, 520, 577, 871
NlaIV GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
NspI RCATGY 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 427
PauI GCGCGC 2 cut(s) 730, 732
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdmI GAANNNNTTC 2 cut(s) 653, 704
Pfl23II CGTACG 1 cut(s) 232
PflFI GACNNNGTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 7 cut(s) 57, 240, 321, 366, 522, 749, 783
PleI GAGTC 4 cut(s) 40, 55, 64, 702
PluTI GGCGCC 1 cut(s) 365
PpsI GAGTC 4 cut(s) 40, 55, 64, 702
Psp124BI GAGCTC 1 cut(s) 64
PspLI CGTACG 1 cut(s) 232
PspN4I GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
PspPI GGNCC 3 cut(s) 274, 723, 742
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 122
PteI GCGCGC 2 cut(s) 730, 732
RsaI GTAC 2 cut(s) 203, 234
RsaNI GTAC 2 cut(s) 202, 233
Rsr2I CGGWCCG 1 cut(s) 274
RsrII CGGWCCG 1 cut(s) 274
SacI GAGCTC 1 cut(s) 64
SacII CCGCGG 1 cut(s) 748
SalI GTCGAC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 810
SatI GCNGC 7 cut(s) 56, 239, 320, 365, 521, 748, 782
Sau3AI GATC 3 cut(s) 106, 146, 462
Sau96I GGNCC 3 cut(s) 274, 723, 742
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 4 cut(s) 41, 56, 64, 703
ScrFI CCNGG 5 cut(s) 416, 617, 645, 646, 660
SduI GDGCHC 2 cut(s) 64, 675
SfaNI GCATC 2 cut(s) 479, 630
SfoI GGCGCC 1 cut(s) 363
Sfr303I CCGCGG 1 cut(s) 748
SgrAI CRCCGGYG 2 cut(s) 13, 367
SgrBI CCGCGG 1 cut(s) 748
SgrDI CGTCGACG 1 cut(s) 101
SinI GGWCC 1 cut(s) 274
SmaI CCCGGG 1 cut(s) 646
Sse9I AATT 3 cut(s) 284, 583, 889
SsiI CCGC 8 cut(s) 319, 365, 386, 609, 629, 745, 747, 781
SspDI GGCGCC 1 cut(s) 361
SspMI CTAG 1 cut(s) 801
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 5 cut(s) 414, 615, 643, 644, 658
StyI CCWWGG 2 cut(s) 528, 573
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 3 cut(s) 41, 92, 126
TaqI TCGA 5 cut(s) 102, 117, 449, 581, 633
TaqII GACCGA 1 cut(s) 291
TasI AATT 3 cut(s) 284, 583, 889
TatI WGTACW 1 cut(s) 201
TauI GCSGC 4 cut(s) 322, 367, 750, 784
Tru1I TTAA 1 cut(s) 810
Tru9I TTAA 1 cut(s) 810
TscAI CASTG 1 cut(s) 866
TseI GCWGC 3 cut(s) 55, 238, 520
TspGWI ACGGA 3 cut(s) 63, 159, 267
TspMI CCCGGG 1 cut(s) 644
TspRI CASTG 1 cut(s) 866
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 1 cut(s) 284
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmaI CCCGGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 2 cut(s) 653, 704
XspI CTAG 1 cut(s) 801
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.