Prupe.2G292100_v2.0.a1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013887
Physical Location & Seq
Reverse (-)
28530389 .. 28531462
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G292100.1

Sequence Viewer

Length: 912 bp
ATGGCTCCGACGTCGGTGGAGCCGAACGGCGGTGAGTCCACATCAGGCGAGTCATCCCAGAGAGCTTTACCGACACCATTTCTGACCAAGACGTATCAGCTGGTCGATGATCCCACAATCGACGACGTCATCTCCTGGAACGACGACGGATCTAGCTTTGTCGTCTGGAACCCCACCGTCTTCGCCAGAGATTTGCTCCCCAAGTATTTCAAGCACAACAACTTCTCTAGCTTCGTCAGGCAGCTCAACACCTACGGATTTAGAAAGGTTATACCAGACCGTTGGGAATTCTCGAACGATTGCTTTCGAAGAGGCGAAAAGCGGCTTCTCTGCGAGATACAGCGCCGAAGAATCATGCCTCCGGCGCCGTCGGTGGCCGTTTCGCCGATGGCGACGGCAGCGGTGGTTCCGAATGCGAAACCTATGATATCTCCGTCGAACTCCGGCGAGGAGCAGGTGATTTCATCGAGCTCGTCGCCGATCAGAGCTCCGTCCGAGCTCATGGACGAGAACGAAAAGCTGAGAAAGGAGAACATGCAGCTCACCAAGGAACTGGCGGAAGTCAAGTCTCTCTGCAACAACATCTTCAGCATGGTCTCGAATTACGCGTACGCACAATCGGAAAGCGGTTTCCCATATGTGAAACCGCTGGATTTGATGCCTGAAAAGCGGTTCTCCGGCGACGGCGAGAAGGAAGAAGAGGAGGCGAGCCCGAAGCTTTTCGGTGTGGCGATTGGAGCCAAGCGAGCAAGAGAGACCGTCGGCGACGGTGTAGAGGAGGATGAGACCGGCTTACGACTGCAGCAACCTAGCGGTGGTGGCGACGTTAAATCAGAGCCGATAGATATGGATCGTCAGGAAACGCCGTGGCTGAATCAGCGCCACAGGGCTAATCAGAGGGTGTGTAATTGA

Protein Analysis

304

Amino Acids

33.9

Weight (kDa)

5.19

Isoelectric Point (pI)

61.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
AatII GACGTC 2 cut(s) 14, 129
Acc36I ACCTGC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 1 cut(s) 608
AciI CCGC 8 cut(s) 30, 322, 401, 557, 627, 647, 670, 813
AclWI GGATC 3 cut(s) 104, 157, 858
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 571
AcyI GRCGYC 3 cut(s) 11, 126, 365
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 2 cut(s) 29, 815
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 134
Alw21I GWGCWC 3 cut(s) 473, 490, 501
Alw26I GTCTC 4 cut(s) 573, 601, 749, 779
AlwI GGATC 3 cut(s) 104, 157, 858
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 4 cut(s) 241, 398, 538, 802
ApoI RAATTY 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 719
AspLEI GCGC 3 cut(s) 345, 367, 882
AsuHPI GGTGA 3 cut(s) 44, 469, 535
AsuII TTCGAA 1 cut(s) 307
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 4 cut(s) 473, 490, 501, 713
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 3 cut(s) 473, 490, 501
BbvI GCAGC 4 cut(s) 253, 410, 550, 814
BccI CCATC 1 cut(s) 382
BceAI ACGGC 6 cut(s) 43, 352, 362, 411, 700, 850
BciT130I CCWGG 1 cut(s) 136
BcoDI GTCTC 4 cut(s) 573, 601, 749, 779
BfaI CTAG 3 cut(s) 153, 228, 810
BfmI CTRYAG 1 cut(s) 800
BfoI RGCGCY 3 cut(s) 346, 368, 883
BfuAI ACCTGC 1 cut(s) 445
BisI GCNGC 5 cut(s) 242, 323, 399, 539, 803
BlsI GCNGC 5 cut(s) 243, 324, 400, 540, 804
Bme1390I CCNGG 1 cut(s) 136
BmiI GGNNCC 6 cut(s) 6, 21, 170, 366, 408, 739
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 648
BpiI GAAGAC 1 cut(s) 172
BplI GAGNNNNNCTC 2 cut(s) 180, 212
Bpu14I TTCGAA 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 849
BsaHI GRCGYC 3 cut(s) 11, 126, 365
BsaI GGTCTC 3 cut(s) 601, 749, 779
BsaJI CCNNGG 2 cut(s) 546, 866
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 815
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 2 cut(s) 546, 866
BseGI GGATG 2 cut(s) 53, 787
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 2 cut(s) 29, 815
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 3 cut(s) 464, 716, 791
BseXI GCAGC 4 cut(s) 253, 410, 550, 814
Bsh1236I CGCG 1 cut(s) 608
BshFI GGCC 1 cut(s) 377
BshNI GGYRCC 1 cut(s) 364
BsiHKAI GWGCWC 3 cut(s) 473, 490, 501
BsiSI CCGG 4 cut(s) 362, 444, 678, 789
BsiWI CGTACG 1 cut(s) 609
BslI CCNNNNNNNGG 2 cut(s) 29, 815
BsmAI GTCTC 4 cut(s) 573, 601, 749, 779
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 1 cut(s) 377
Bso31I GGTCTC 3 cut(s) 601, 749, 779
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 4 cut(s) 473, 490, 501, 713
Bsp143I GATC 4 cut(s) 109, 149, 480, 850
BspACI CCGC 8 cut(s) 30, 322, 401, 557, 627, 647, 670, 813
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 1 cut(s) 513
BspFNI CGCG 1 cut(s) 608
BspLI GGNNCC 6 cut(s) 6, 21, 170, 366, 408, 739
BspMAI CTGCAG 1 cut(s) 804
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 3 cut(s) 104, 157, 858
BspT104I TTCGAA 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 3 cut(s) 601, 749, 779
BsrFI RCCGGY 1 cut(s) 788
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 2 cut(s) 546, 866
BssMI GATC 4 cut(s) 109, 149, 480, 850
BssNI GRCGYC 3 cut(s) 11, 126, 365
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 4 cut(s) 178, 281, 760, 770
Bst6I CTCTTC 2 cut(s) 304, 693
BstACI GRCGYC 3 cut(s) 11, 126, 365
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 709, 747
BstDEI CTNAG 1 cut(s) 521
BstDSI CCRYGG 1 cut(s) 866
BstF5I GGATG 2 cut(s) 53, 787
BstFNI CGCG 1 cut(s) 608
BstH2I RGCGCY 3 cut(s) 346, 368, 883
BstHHI GCGC 3 cut(s) 345, 367, 882
BstKTI GATC 4 cut(s) 112, 152, 483, 853
BstMAI GTCTC 4 cut(s) 573, 601, 749, 779
BstMBI GATC 4 cut(s) 109, 149, 480, 850
BstMWI GCNNNNNNNGC 7 cut(s) 364, 398, 667, 737, 746, 819, 877
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 800
BstUI CGCG 1 cut(s) 608
BstV1I GCAGC 4 cut(s) 253, 410, 550, 814
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 2 cut(s) 282, 553
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 1 cut(s) 377
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 2 cut(s) 53, 787
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 2 cut(s) 709, 747
CfoI GCGC 3 cut(s) 345, 367, 882
Cfr10I RCCGGY 1 cut(s) 788
Csp6I GTAC 1 cut(s) 610
CviAII CATG 4 cut(s) 355, 502, 535, 592
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 1 cut(s) 521
DinI GGCGCC 1 cut(s) 366
DpnI GATC 4 cut(s) 111, 151, 482, 852
DpnII GATC 4 cut(s) 109, 149, 480, 850
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 2 cut(s) 304, 693
EarI CTCTTC 2 cut(s) 304, 693
EciI GGCGGA 1 cut(s) 572
Ecl136II GAGCTC 3 cut(s) 471, 488, 499
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 4 cut(s) 473, 490, 501, 713
Eco31I GGTCTC 3 cut(s) 601, 749, 779
Eco32I GATATC 1 cut(s) 429
Eco53kI GAGCTC 3 cut(s) 471, 488, 499
Eco57I CTGAAG 1 cut(s) 571
EcoICRI GAGCTC 3 cut(s) 471, 488, 499
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 134
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 4 cut(s) 473, 490, 501, 713
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 4 cut(s) 358, 505, 538, 595
FaiI YATR 9 cut(s) 272, 356, 425, 503, 536, 593, 637, 639, 848
FatI CATG 4 cut(s) 354, 501, 534, 591
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 5 cut(s) 242, 323, 399, 539, 803
FokI GGATG 2 cut(s) 40, 794
FriOI GRGCYC 4 cut(s) 473, 490, 501, 713
Fsp4HI GCNGC 5 cut(s) 242, 323, 399, 539, 803
FspBI CTAG 3 cut(s) 153, 228, 810
GlaI GCGC 3 cut(s) 344, 366, 881
GluI GCNGC 5 cut(s) 242, 323, 399, 539, 803
HaeII RGCGCY 3 cut(s) 346, 368, 883
HaeIII GGCC 1 cut(s) 377
HapII CCGG 4 cut(s) 362, 444, 678, 789
HhaI GCGC 3 cut(s) 345, 367, 882
Hin1I GRCGYC 3 cut(s) 11, 126, 365
Hin1II CATG 4 cut(s) 358, 505, 538, 595
Hin6I GCGC 3 cut(s) 343, 365, 880
HinP1I GCGC 3 cut(s) 343, 365, 880
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 4 cut(s) 35, 50, 351, 874
HpaII CCGG 4 cut(s) 362, 444, 678, 789
HphI GGTGA 3 cut(s) 44, 469, 535
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 8 cut(s) 9, 84, 411, 485, 496, 622, 835, 897
Hpy188III TCNNGA 4 cut(s) 166, 292, 598, 857
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 685
HpyCH4III ACNGT 4 cut(s) 178, 281, 760, 770
HpyCH4IV ACGT 4 cut(s) 11, 92, 126, 825
HpyCH4V TGCA 3 cut(s) 538, 576, 802
HpyF10VI GCNNNNNNNGC 7 cut(s) 364, 398, 667, 737, 746, 819, 877
HpyF3I CTNAG 1 cut(s) 521
HpySE526I ACGT 4 cut(s) 11, 92, 126, 825
Hsp92I GRCGYC 3 cut(s) 11, 126, 365
Hsp92II CATG 4 cut(s) 358, 505, 538, 595
HspAI GCGC 3 cut(s) 343, 365, 880
KasI GGCGCC 1 cut(s) 364
Kzo9I GATC 4 cut(s) 109, 149, 480, 850
LmnI GCTCC 6 cut(s) 10, 19, 201, 451, 493, 737
Lsp1109I GCAGC 4 cut(s) 253, 410, 550, 814
LweI GCATC 1 cut(s) 648
MaeI CTAG 3 cut(s) 153, 228, 810
MaeII ACGT 4 cut(s) 11, 92, 126, 825
MalI GATC 4 cut(s) 111, 151, 482, 852
MboI GATC 4 cut(s) 109, 149, 480, 850
MboII GAAGA 6 cut(s) 172, 321, 360, 577, 707, 710
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 4 cut(s) 473, 490, 501, 713
MluCI AATT 3 cut(s) 287, 601, 907
MluI ACGCGT 1 cut(s) 606
Mly113I GGCGCC 1 cut(s) 365
MlyI GAGTC 2 cut(s) 44, 59
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 8 cut(s) 305, 369, 442, 694, 697, 769, 772, 891
MroXI GAANNNNTTC 1 cut(s) 719
MseI TTAA 1 cut(s) 828
MspA1I CMGCKG 3 cut(s) 100, 401, 649
MspI CCGG 4 cut(s) 362, 444, 678, 789
MspR9I CCNGG 1 cut(s) 136
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 608
MwoI GCNNNNNNNGC 7 cut(s) 364, 398, 667, 737, 746, 819, 877
NarI GGCGCC 1 cut(s) 365
NdeI CATATG 1 cut(s) 637
NdeII GATC 4 cut(s) 109, 149, 480, 850
NlaIII CATG 4 cut(s) 358, 505, 538, 595
NlaIV GGNNCC 6 cut(s) 6, 21, 170, 366, 408, 739
NspI RCATGY 1 cut(s) 538
NspV TTCGAA 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 389, 605
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 2 cut(s) 351, 874
Pfl23II CGTACG 1 cut(s) 609
PflFI GACNNNGTC 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 134
PkrI GCNGC 5 cut(s) 243, 324, 400, 540, 804
PleI GAGTC 2 cut(s) 43, 58
PluTI GGCGCC 1 cut(s) 368
PpsI GAGTC 2 cut(s) 43, 58
Psp124BI GAGCTC 3 cut(s) 473, 490, 501
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspLI CGTACG 1 cut(s) 609
PspN4I GGNNCC 6 cut(s) 6, 21, 170, 366, 408, 739
PstI CTGCAG 1 cut(s) 804
PsuI RGATCY 1 cut(s) 149
PsyI GACNNNGTC 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
SacI GAGCTC 3 cut(s) 473, 490, 501
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 5 cut(s) 242, 323, 399, 539, 803
Sau3AI GATC 4 cut(s) 109, 149, 480, 850
SchI GAGTC 2 cut(s) 44, 59
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 4 cut(s) 473, 490, 501, 713
SfaNI GCATC 1 cut(s) 648
SfcI CTRYAG 1 cut(s) 800
SfoI GGCGCC 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 307
Sse9I AATT 3 cut(s) 287, 601, 907
SsiI CCGC 8 cut(s) 30, 322, 401, 557, 627, 647, 670, 813
SspDI GGCGCC 1 cut(s) 364
SspMI CTAG 3 cut(s) 153, 228, 810
SstI GAGCTC 3 cut(s) 473, 490, 501
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 4 cut(s) 178, 281, 760, 770
TaiI ACGT 4 cut(s) 14, 95, 129, 828
TaqI TCGA 7 cut(s) 105, 120, 293, 307, 437, 467, 599
TasI AATT 3 cut(s) 287, 601, 907
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 2 cut(s) 351, 874
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TseI GCWGC 4 cut(s) 241, 398, 538, 802
TspDTI ATGAA 1 cut(s) 453
TspGWI ACGGA 4 cut(s) 162, 270, 423, 480
Tth111I GACNNNGTC 1 cut(s) 125
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 3 cut(s) 153, 228, 810
ZraI GACGTC 2 cut(s) 12, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.