Rh1AG417100

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0013887
Physical Location & Seq
Reverse (-)
64915528 .. 64916858
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG417100.1

Sequence Viewer

Length: 870 bp
ATGGCTCCTCCGCCTACGGTGGAGCAGAACGGCGAGTCCGCCTCCGGCGAGTCGACGCAGCGAGGCTCTCCGACGCCGTTTCTGAGCAAAACGTACCAGCTCGTCGACGATCCCGCCATCGATGACGTCATCGCCTGGAGCGGCGACGGATCCAGCTTCGTGGTGAAGAACCCCACCGTGTTCGCCAGAGATTTGCTGCCCAAGTATTTCAAGCACAACAACTTCTCCAGCTTCGTCCGGCAGCTCAACACCTACGGATTTCGGAAGATTATACCTGACCGGTGGGAATTCTCCAACGACTGCTTCCGCAGAGGCGAGAAGCGGCTGCTCTGCGAAATACAGCGGCGGAGAATCACGCCTCCGGCGGCTGCTCCGGCCGTGATTCCGGCGAAACCTATCACCTCTCCGGCGAACTCCGGCGAGGAGCAGGTGACTACTTCGTCGAGCTCCTCGCCGCGGCCGAGGATTCCGTGGCAGCTGGTGGAGGAGAACGAGAGGCTGAGGAAGGAGAACCTCCGGCTGACCAAGGAGCTGGCGGAGATCAAGTCTCTCTGCCACAACATCTTCTCCCTGGTGTCGAATTACGCCTGTAATAAGCCACCGGAGGGCGGTTTAGGTGAGTTGACACAGCTGAGTTTGATGCCCGGGGAGCGGTTTTCCGGCGGGGAGACGAGTCCGAAGATTTTCGGGGTGGAAATCGGAGCGAAGCGGGCGAGGGAGGAAGGCGGTGGAGGCGATGAGGAGACGGATCTACGGCTGCGGCAACCGAGCGGTGGTGGCGTTGTGAAATCAGAGCCGTCCGATGTGGAGCATCGTGAGAAGCCGTGGCTGAAGCAGTGCCACAGGGCTAATCAGAGGGTGTGTAATTGA

Protein Analysis

289

Amino Acids

32.12

Weight (kDa)

8.5

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 27 - 116 2.6e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 418
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 418
AccBSI CCGCTC 3 cut(s) 141, 652, 771
AccI GTMKAC 2 cut(s) 53, 105
AccII CGCG 1 cut(s) 457
AclWI GGATC 4 cut(s) 104, 144, 157, 756
AcoI YGGCCR 2 cut(s) 375, 458
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 851
AcyI GRCGYC 2 cut(s) 74, 126
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 5 cut(s) 456, 605, 608, 651, 773
AgeI ACCGGT 1 cut(s) 279
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 2 cut(s) 134, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AloI GAACNNNNNNTCC 2 cut(s) 20, 52
AluBI AGCT 8 cut(s) 100, 156, 231, 244, 447, 478, 532, 631
AluI AGCT 8 cut(s) 100, 156, 231, 244, 447, 478, 532, 631
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 3 cut(s) 552, 662, 737
AlwI GGATC 4 cut(s) 104, 144, 157, 756
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 2 cut(s) 375, 458
ApeKI GCWGC 7 cut(s) 58, 196, 241, 325, 368, 475, 757
ApoI RAATTY 1 cut(s) 287
AsiGI ACCGGT 1 cut(s) 279
Asp700I GAANNNNTTC 1 cut(s) 683
AsuC2I CCSGG 2 cut(s) 645, 646
AsuHPI GGTGA 4 cut(s) 175, 391, 442, 629
AvaI CYCGRG 1 cut(s) 644
BamHI GGATCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 449
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 7 cut(s) 70, 183, 253, 312, 355, 487, 744
BccI CCATC 1 cut(s) 125
BceAI ACGGC 6 cut(s) 46, 61, 362, 770, 781, 808
BciT130I CCWGG 2 cut(s) 136, 572
BcnI CCSGG 2 cut(s) 645, 646
BcoDI GTCTC 3 cut(s) 552, 662, 737
BfuAI ACCTGC 1 cut(s) 418
Bme1390I CCNGG 4 cut(s) 136, 572, 645, 646
BmeT110I CYCGRG 1 cut(s) 644
BmiI GGNNCC 2 cut(s) 6, 151
BmrFI CCNGG 4 cut(s) 136, 572, 645, 646
BmsI GCATC 2 cut(s) 630, 820
BplI GAGNNNNNCTC 2 cut(s) 26, 58
BpmI CTGGAG 2 cut(s) 157, 211
Bpu10I CCTNAGC 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 645, 646
Bsa29I ATCGAT 1 cut(s) 120
BsaHI GRCGYC 2 cut(s) 74, 126
BsaJI CCNNGG 8 cut(s) 455, 461, 470, 525, 570, 644, 645, 824
BsaWI WCCGGW 2 cut(s) 279, 601
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 530, 551, 560, 581
Bsc4I CCNNNNNNNGG 5 cut(s) 456, 605, 608, 651, 773
Bse118I RCCGGY 1 cut(s) 279
BseBI CCWGG 2 cut(s) 136, 572
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 8 cut(s) 455, 461, 470, 525, 570, 644, 645, 824
BseLI CCNNNNNNNGG 5 cut(s) 456, 605, 608, 651, 773
BseMII CTCAG 3 cut(s) 74, 491, 623
BseRI GAGGAG 4 cut(s) 437, 439, 500, 755
BseX3I CGGCCG 2 cut(s) 375, 458
BseXI GCAGC 7 cut(s) 70, 183, 253, 312, 355, 487, 744
Bsh1236I CGCG 1 cut(s) 457
Bsh1285I CGRYCG 2 cut(s) 378, 461
BshFI GGCC 2 cut(s) 377, 460
BshTI ACCGGT 1 cut(s) 279
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 2 cut(s) 378, 461
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 644
BslI CCNNNNNNNGG 5 cut(s) 456, 605, 608, 651, 773
BsmAI GTCTC 3 cut(s) 552, 662, 737
BsmBI CGTCTC 2 cut(s) 662, 737
BsnI GGCC 2 cut(s) 377, 460
BsoBI CYCGRG 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 4 cut(s) 109, 149, 540, 748
BspANI GGCC 2 cut(s) 377, 460
BspCNI CTCAG 3 cut(s) 75, 492, 624
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 6, 151
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 4 cut(s) 104, 144, 157, 756
BsrBI CCGCTC 3 cut(s) 141, 652, 771
BsrFI RCCGGY 1 cut(s) 279
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 8 cut(s) 455, 461, 470, 525, 570, 644, 645, 824
BssMI GATC 4 cut(s) 109, 149, 540, 748
BssNI GRCGYC 2 cut(s) 74, 126
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 136, 572
Bst4CI ACNGT 2 cut(s) 19, 178
BstACI GRCGYC 2 cut(s) 74, 126
BstC8I GCNNGC 2 cut(s) 534, 711
BstDEI CTNAG 3 cut(s) 83, 500, 632
BstDSI CCRYGG 3 cut(s) 455, 470, 824
BstFNI CGCG 1 cut(s) 457
BstKTI GATC 4 cut(s) 112, 152, 543, 751
BstMAI GTCTC 3 cut(s) 552, 662, 737
BstMBI GATC 4 cut(s) 109, 149, 540, 748
BstMCI CGRYCG 2 cut(s) 378, 461
BstMWI GCNNNNNNNGC 5 cut(s) 374, 649, 710, 732, 777
BstNI CCWGG 2 cut(s) 136, 572
BstSCI CCNGG 4 cut(s) 134, 570, 643, 644
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 7 cut(s) 70, 183, 253, 312, 355, 487, 744
BstX2I RGATCY 2 cut(s) 149, 748
BstXI CCANNNNNNTGG 2 cut(s) 160, 532
BstYI RGATCY 2 cut(s) 149, 748
BstZI CGGCCG 2 cut(s) 375, 458
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 2 cut(s) 377, 460
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 3 cut(s) 455, 470, 824
BtgZI GCGATG 2 cut(s) 115, 750
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 1 cut(s) 842
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 2 cut(s) 534, 711
Cfr10I RCCGGY 1 cut(s) 279
Cfr42I CCGCGG 1 cut(s) 458
Cfr9I CCCGGG 1 cut(s) 644
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 2 cut(s) 64, 82
Csp6I GTAC 1 cut(s) 94
CspAI ACCGGT 1 cut(s) 279
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 3 cut(s) 83, 500, 632
DpnI GATC 4 cut(s) 111, 151, 542, 750
DpnII GATC 4 cut(s) 109, 149, 540, 748
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 375, 458
EagI CGGCCG 2 cut(s) 375, 458
EciI GGCGGA 3 cut(s) 28, 361, 551
Ecl136II GAGCTC 1 cut(s) 447
EclXI CGGCCG 2 cut(s) 375, 458
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 449
Eco52I CGGCCG 2 cut(s) 375, 458
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 851
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 134, 570
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 662, 737
FaiI YATR 1 cut(s) 272
FauI CCCGC 3 cut(s) 121, 656, 702
FblI GTMKAC 2 cut(s) 53, 105
FriOI GRGCYC 1 cut(s) 449
GsuI CTGGAG 2 cut(s) 157, 211
HaeIII GGCC 2 cut(s) 377, 460
HgaI GACGC 2 cut(s) 64, 82
Hin1I GRCGYC 2 cut(s) 74, 126
HincII GTYRAC 3 cut(s) 54, 106, 624
HindII GTYRAC 3 cut(s) 54, 106, 624
HinfI GANTC 6 cut(s) 35, 50, 351, 382, 466, 673
HphI GGTGA 4 cut(s) 175, 391, 442, 629
Hpy166II GTNNAC 3 cut(s) 54, 106, 624
Hpy188I TCNGA 8 cut(s) 72, 84, 264, 678, 701, 793, 802, 855
Hpy188III TCNNGA 1 cut(s) 815
Hpy8I GTNNAC 3 cut(s) 54, 106, 624
Hpy99I CGWCG 6 cut(s) 58, 76, 107, 110, 149, 445
HpyAV CCTTC 2 cut(s) 499, 716
HpyCH4III ACNGT 2 cut(s) 19, 178
HpyCH4IV ACGT 2 cut(s) 92, 126
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 649, 710, 732, 777
HpyF3I CTNAG 3 cut(s) 83, 500, 632
HpySE526I ACGT 2 cut(s) 92, 126
Hsp92I GRCGYC 2 cut(s) 74, 126
KspI CCGCGG 1 cut(s) 458
Kzo9I GATC 4 cut(s) 109, 149, 540, 748
Lsp1109I GCAGC 7 cut(s) 70, 183, 253, 312, 355, 487, 744
LweI GCATC 2 cut(s) 630, 820
MaeII ACGT 2 cut(s) 92, 126
MaeIII GTNAC 1 cut(s) 430
MalI GATC 4 cut(s) 111, 151, 542, 750
MbiI CCGCTC 3 cut(s) 141, 652, 771
MboI GATC 4 cut(s) 109, 149, 540, 748
MboII GAAGA 4 cut(s) 178, 277, 556, 691
MflI RGATCY 2 cut(s) 149, 748
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 3 cut(s) 287, 580, 865
MlyI GAGTC 3 cut(s) 44, 59, 682
MmeI TCCRAC 2 cut(s) 95, 318
MroXI GAANNNNTTC 1 cut(s) 683
MspA1I CMGCKG 4 cut(s) 343, 457, 478, 631
MspR9I CCNGG 4 cut(s) 136, 572, 645, 646
MvaI CCWGG 2 cut(s) 136, 572
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 5 cut(s) 374, 649, 710, 732, 777
NciI CCSGG 2 cut(s) 645, 646
NdeII GATC 4 cut(s) 109, 149, 540, 748
NlaIV GGNNCC 2 cut(s) 6, 151
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 3 cut(s) 351, 382, 466
PinAI ACCGGT 1 cut(s) 279
PleI GAGTC 3 cut(s) 43, 58, 681
PpsI GAGTC 3 cut(s) 43, 58, 681
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 134, 570
PspGI CCWGG 2 cut(s) 134, 570
PspN4I GGNNCC 2 cut(s) 6, 151
PsuI RGATCY 2 cut(s) 149, 748
PvuII CAGCTG 2 cut(s) 478, 631
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacI GAGCTC 1 cut(s) 449
SacII CCGCGG 1 cut(s) 458
SalI GTCGAC 2 cut(s) 52, 104
Sau3AI GATC 4 cut(s) 109, 149, 540, 748
SchI GAGTC 3 cut(s) 44, 59, 682
ScrFI CCNGG 4 cut(s) 136, 572, 645, 646
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 2 cut(s) 630, 820
Sfr303I CCGCGG 1 cut(s) 458
SgrBI CCGCGG 1 cut(s) 458
SgrDI CGTCGACG 1 cut(s) 104
SmaI CCCGGG 1 cut(s) 646
Sse9I AATT 3 cut(s) 287, 580, 865
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 4 cut(s) 134, 570, 643, 644
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 19, 178
TaiI ACGT 2 cut(s) 95, 129
TaqI TCGA 5 cut(s) 53, 105, 120, 443, 578
TasI AATT 3 cut(s) 287, 580, 865
TauI GCSGC 7 cut(s) 144, 325, 346, 368, 457, 460, 763
TfiI GAWTC 3 cut(s) 351, 382, 466
TscAI CASTG 1 cut(s) 842
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 7 cut(s) 58, 196, 241, 325, 368, 475, 757
Tsp45I GTSAC 1 cut(s) 430
TspGWI ACGGA 4 cut(s) 162, 270, 459, 761
TspMI CCCGGG 1 cut(s) 644
TspRI CASTG 1 cut(s) 842
XapI RAATTY 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 644
XmiI GTMKAC 2 cut(s) 53, 105
XmnI GAANNNNTTC 1 cut(s) 683
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.