MD01G1199100.v1.1

Protein phosphatase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008626
Physical Location & Seq
Reverse (-)
29648218 .. 29650790
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1199100.v1.1.491

Sequence Viewer

Length: 561 bp
ATGAAAGGCCGAGTTAGATGGGACGAGGCTAATATTGGAGAAATTGAAGCAAATAAACCTGTAAGGCAGAAAATTACTGAGCCGAAGACGCCATACCACCCCATGATGACCGATGATGAGTATGGATCACTGTCTCCTATGCGGGGCGGTACCTTCGATGATTGTGTTGGTGATGCAGATCATGCAGAAGCAATACGAACTGCTTTGAGTGATGTGGCGTCATCAAGTAGAAAGAGCACCCAACGGTCTACTGGCTGGACATCATCTGAGGATGAGGCCGATACTATGGAACAAGATGATGAAGATTCCGAGAAAAGCAAGAGTTTTAGGGAGCACAGAAGAGCCCATTATGATGAATTCCAGAAAGTAAAAGAACTCAGGCGGAAAGCTTCTTTGCTCGACGATGAAGAAGAGGACGAGGATGAGAATGTTGACATGGATAAGAAGGAGAAGAAATCTGGTTCCTCTTCGTTAACTGCCGGTGTCAAAGAAATAGATATCGACGAAGATGGTACCTCATCAACCAAAAACTCTTCCGTACCTCCGGCGAATGGCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IPP-2

Protein Analysis

187

Amino Acids

20.77

Weight (kDa)

4.65

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPP-2 PF04979 5 - 138 3.6e-24 Protein phosphatase inhibitor 2 (IPP-2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 149, 512
AccB1I GGYRCC 2 cut(s) 149, 512
AccI GTMKAC 1 cut(s) 248
AciI CCGC 3 cut(s) 142, 147, 382
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 1 cut(s) 356
AcyI GRCGYC 2 cut(s) 89, 218
AfaI GTAC 3 cut(s) 151, 514, 540
AfiI CCNNNNNNNGG 2 cut(s) 143, 551
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
Alw21I GWGCWC 2 cut(s) 239, 336
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 133
AoxI GGCC 2 cut(s) 7, 276
ApoI RAATTY 1 cut(s) 356
Asp718I GGTACC 2 cut(s) 149, 512
AspLEI GCGC 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 182
BanI GGYRCC 2 cut(s) 149, 512
BanII GRGCYC 1 cut(s) 346
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 2 cut(s) 239, 336
BccI CCATC 2 cut(s) 12, 503
BcoDI GTCTC 1 cut(s) 138
BfoI RGCGCY 1 cut(s) 558
BmiI GGNNCC 3 cut(s) 151, 463, 514
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 2 cut(s) 89, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 551
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 177
BseGI GGATG 2 cut(s) 277, 427
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 143, 551
BseMII CTCAG 3 cut(s) 69, 258, 391
BseNI ACTGG 1 cut(s) 256
BshFI GGCC 2 cut(s) 9, 278
BshNI GGYRCC 2 cut(s) 149, 512
BsiHKAI GWGCWC 2 cut(s) 239, 336
BsiSI CCGG 2 cut(s) 480, 545
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 143, 551
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 2 cut(s) 9, 278
Bsp1286I GDGCHC 3 cut(s) 239, 336, 346
Bsp143I GATC 2 cut(s) 125, 178
BspACI CCGC 3 cut(s) 142, 147, 382
BspANI GGCC 2 cut(s) 9, 278
BspCNI CTCAG 3 cut(s) 70, 259, 390
BspLI GGNNCC 3 cut(s) 151, 463, 514
BspPI GGATC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 334
BspT107I GGYRCC 2 cut(s) 149, 512
BsrFI RCCGGY 1 cut(s) 479
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 479
BssMI GATC 2 cut(s) 125, 178
BssNI GRCGYC 2 cut(s) 89, 218
Bst4CI ACNGT 2 cut(s) 132, 246
Bst6I CTCTTC 4 cut(s) 334, 405, 472, 538
BstACI GRCGYC 2 cut(s) 89, 218
BstAPI GCANNNNNTGC 1 cut(s) 182
BstDEI CTNAG 4 cut(s) 78, 267, 377, 558
BstF5I GGATG 2 cut(s) 277, 427
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 2 cut(s) 128, 181
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 125, 178
BstMWI GCNNNNNNNGC 3 cut(s) 88, 182, 554
BstV2I GAAGAC 1 cut(s) 92
BsuRI GGCC 2 cut(s) 9, 278
BtsCI GGATG 2 cut(s) 277, 427
BtsIMutI CAGTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 557
Cfr10I RCCGGY 1 cut(s) 479
CseI GACGC 2 cut(s) 97, 207
Csp6I GTAC 3 cut(s) 150, 513, 539
CviAII CATG 3 cut(s) 103, 182, 436
CviJI RGCY 7 cut(s) 9, 29, 82, 255, 278, 344, 389
CviKI_1 RGCY 7 cut(s) 9, 29, 82, 255, 278, 344, 389
CviQI GTAC 3 cut(s) 150, 513, 539
DdeI CTNAG 4 cut(s) 78, 267, 377, 558
DpnI GATC 2 cut(s) 127, 180
DpnII GATC 2 cut(s) 125, 178
Eam1104I CTCTTC 4 cut(s) 334, 405, 472, 538
EarI CTCTTC 4 cut(s) 334, 405, 472, 538
EciI GGCGGA 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 346
Eco32I GATATC 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 356
EcoRV GATATC 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 346
FaeI CATG 3 cut(s) 106, 185, 439
FaiI YATR 8 cut(s) 94, 104, 123, 140, 183, 287, 351, 437
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 102, 181, 435
FauI CCCGC 1 cut(s) 135
FblI GTMKAC 1 cut(s) 248
FokI GGATG 2 cut(s) 284, 434
FriOI GRGCYC 1 cut(s) 346
GlaI GCGC 1 cut(s) 556
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 2 cut(s) 9, 278
HapII CCGG 2 cut(s) 480, 545
HgaI GACGC 2 cut(s) 97, 207
HhaI GCGC 1 cut(s) 557
Hin1I GRCGYC 2 cut(s) 89, 218
Hin1II CATG 3 cut(s) 106, 185, 439
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HincII GTYRAC 2 cut(s) 433, 474
HindII GTYRAC 2 cut(s) 433, 474
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 1 cut(s) 305
HpaI GTTAAC 1 cut(s) 474
HpaII CCGG 2 cut(s) 480, 545
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 3 cut(s) 249, 433, 474
Hpy188I TCNGA 2 cut(s) 268, 310
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 3 cut(s) 249, 433, 474
Hpy99I CGWCG 2 cut(s) 404, 506
HpyAV CCTTC 2 cut(s) 163, 439
HpyCH4III ACNGT 2 cut(s) 132, 246
HpyCH4V TGCA 2 cut(s) 176, 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 182, 554
HpyF3I CTNAG 4 cut(s) 78, 267, 377, 558
Hsp92I GRCGYC 2 cut(s) 89, 218
Hsp92II CATG 3 cut(s) 106, 185, 439
HspAI GCGC 1 cut(s) 555
KpnI GGTACC 2 cut(s) 153, 516
KspAI GTTAAC 1 cut(s) 474
Kzo9I GATC 2 cut(s) 125, 178
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 7 cut(s) 72, 237, 241, 364, 374, 444, 493
LweI GCATC 1 cut(s) 163
MalI GATC 2 cut(s) 127, 180
MboI GATC 2 cut(s) 125, 178
MboII GAAGA 9 cut(s) 97, 314, 351, 419, 422, 459, 463, 518, 525
MhlI GDGCHC 3 cut(s) 239, 336, 346
MluCI AATT 3 cut(s) 42, 72, 356
MnlI CCTC 8 cut(s) 19, 262, 268, 406, 412, 475, 526, 552
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 351
MspI CCGG 2 cut(s) 480, 545
MwoI GCNNNNNNNGC 3 cut(s) 88, 182, 554
NdeII GATC 2 cut(s) 125, 178
NlaIII CATG 3 cut(s) 106, 185, 439
NlaIV GGNNCC 3 cut(s) 151, 463, 514
NmeAIII GCCGAG 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 151, 463, 514
RsaI GTAC 3 cut(s) 151, 514, 540
RsaNI GTAC 3 cut(s) 150, 513, 539
RseI CAYNNNNRTG 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 473
Sau3AI GATC 2 cut(s) 125, 178
SduI GDGCHC 3 cut(s) 239, 336, 346
SetI ASST 5 cut(s) 61, 155, 391, 518, 544
SfaNI GCATC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 3 cut(s) 42, 72, 356
SsiI CCGC 3 cut(s) 142, 147, 382
SspI AATATT 1 cut(s) 34
TaaI ACNGT 2 cut(s) 132, 246
TaqI TCGA 3 cut(s) 156, 399, 501
TaqII GACCGA 1 cut(s) 125
TasI AATT 3 cut(s) 42, 72, 356
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 135
TspDTI ATGAA 4 cut(s) 17, 315, 369, 420
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 135
XapI RAATTY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.