MD07G1266900.v1.1

Protein phosphatase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008626
Physical Location & Seq
Reverse (-)
33499972 .. 33502148
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1266900.v1.1.491

Sequence Viewer

Length: 555 bp
ATGAAAGGCCGAGTTAGATGGGACGAGGATAATATAGGAGAAATTGAAGCAAATAAACCTGTGAGACAGAAAATTACTGAGCCGAAGACACCATACCACCCCATGATGACGGATGATGAGGATGGATCTCTGTCTCCTATACGAGGGGGTGGTACCTTCGACGATTGTGTTGGTGATGCAGATCATGCAGAAGCAATACGAACTGCTTTGTGTGATGTGGCATCATCAAGTAGAAAGAACGCGCTACAATCTACTGGCTGGACATCATCTGAAGATGAGGCAGAAGCTATGGAACAAGATGATGAAGATTCTGAGAAAAGCAAGAGTTTTAGGGAGCTCAGAAGAGCCCATTATGATGAATTCCAGAAAGTAAAAGAACTCAGGCGGAAAGGTTCTTTGCTCGACGATGAAGAAGAGGACGAGGTCGTTAACATGGATAAGGAGAAGAAATCTGGCTCCTCTTCGTTAACTGCCGGTGTCAAAGAAATAGATATCGATGAAGATGGTACCGCATCAACCAAAACCTTTTCCGGACCTCCGGCGACCGGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IPP-2

Protein Analysis

185

Amino Acids

20.31

Weight (kDa)

4.55

Isoelectric Point (pI)

63.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPP-2 PF04979 5 - 139 3.9e-25 Protein phosphatase inhibitor 2 (IPP-2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 152, 506
AccB1I GGYRCC 2 cut(s) 152, 506
AccII CGCG 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 530
AciI CCGC 2 cut(s) 385, 510
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 2 cut(s) 154, 508
AfiI CCNNNNNNNGG 2 cut(s) 143, 545
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 3 cut(s) 287, 337, 551
AluI AGCT 3 cut(s) 287, 337, 551
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 58, 138
AlwI GGATC 1 cut(s) 133
Aor13HI TCCGGA 1 cut(s) 530
AoxI GGCC 1 cut(s) 7
ApoI RAATTY 1 cut(s) 359
Asp718I GGTACC 2 cut(s) 152, 506
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 1 cut(s) 533
BanI GGYRCC 2 cut(s) 152, 506
BanII GRGCYC 2 cut(s) 339, 349
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 339
BccI CCATC 3 cut(s) 12, 116, 497
BcoDI GTCTC 2 cut(s) 58, 138
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 3 cut(s) 154, 457, 508
BmsI GCATC 3 cut(s) 166, 230, 521
BpiI GAAGAC 1 cut(s) 92
Bsa29I ATCGAT 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 180
BsaWI WCCGGW 2 cut(s) 530, 545
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 545
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 530
BseCI ATCGAT 1 cut(s) 495
BseGI GGATG 2 cut(s) 118, 127
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 143, 545
BseMII CTCAG 4 cut(s) 69, 303, 352, 394
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 1 cut(s) 9
BshNI GGYRCC 2 cut(s) 152, 506
BshVI ATCGAT 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 4 cut(s) 474, 531, 539, 546
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 143, 545
BsmAI GTCTC 2 cut(s) 58, 138
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 339, 349
Bsp13I TCCGGA 1 cut(s) 530
Bsp143I GATC 2 cut(s) 125, 181
BspACI CCGC 2 cut(s) 385, 510
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 4 cut(s) 70, 304, 351, 393
BspDI ATCGAT 1 cut(s) 495
BspEI TCCGGA 1 cut(s) 530
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 3 cut(s) 154, 457, 508
BspPI GGATC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 337
BspT107I GGYRCC 2 cut(s) 152, 506
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 473
BssMI GATC 2 cut(s) 125, 181
Bst6I CTCTTC 3 cut(s) 337, 408, 466
BstAPI GCANNNNNTGC 1 cut(s) 185
BstDEI CTNAG 5 cut(s) 78, 312, 338, 380, 552
BstENI CCTNNNNNAGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 118, 127
BstFNI CGCG 1 cut(s) 242
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 2 cut(s) 128, 184
BstMAI GTCTC 2 cut(s) 58, 138
BstMBI GATC 2 cut(s) 125, 181
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 2 cut(s) 185, 548
BstUI CGCG 1 cut(s) 242
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
Bsu15I ATCGAT 1 cut(s) 495
BsuRI GGCC 1 cut(s) 9
BsuTUI ATCGAT 1 cut(s) 495
BtsCI GGATG 2 cut(s) 118, 127
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 533
ClaI ATCGAT 1 cut(s) 495
Csp6I GTAC 2 cut(s) 153, 507
CviAII CATG 3 cut(s) 103, 185, 433
CviJI RGCY 8 cut(s) 9, 82, 258, 287, 337, 347, 456, 551
CviKI_1 RGCY 8 cut(s) 9, 82, 258, 287, 337, 347, 456, 551
CviQI GTAC 2 cut(s) 153, 507
DdeI CTNAG 5 cut(s) 78, 312, 338, 380, 552
DpnI GATC 2 cut(s) 127, 183
DpnII GATC 2 cut(s) 125, 181
Eam1104I CTCTTC 3 cut(s) 337, 408, 466
EarI CTCTTC 3 cut(s) 337, 408, 466
EciI GGCGGA 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 339, 349
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 359
EcoRV GATATC 1 cut(s) 493
EcoT38I GRGCYC 2 cut(s) 339, 349
FaeI CATG 3 cut(s) 106, 188, 436
FaiI YATR 8 cut(s) 35, 94, 104, 140, 186, 290, 354, 434
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 102, 184, 432
FokI GGATG 2 cut(s) 125, 134
FriOI GRGCYC 2 cut(s) 339, 349
GlaI GCGC 1 cut(s) 243
HaeIII GGCC 1 cut(s) 9
HapII CCGG 4 cut(s) 474, 531, 539, 546
HhaI GCGC 1 cut(s) 244
Hin1II CATG 3 cut(s) 106, 188, 436
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 2 cut(s) 430, 468
HindII GTYRAC 2 cut(s) 430, 468
HinfI GANTC 1 cut(s) 308
HpaI GTTAAC 2 cut(s) 430, 468
HpaII CCGG 4 cut(s) 474, 531, 539, 546
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 2 cut(s) 430, 468
Hpy188I TCNGA 3 cut(s) 271, 313, 341
Hpy188III TCNNGA 2 cut(s) 364, 531
Hpy8I GTNNAC 2 cut(s) 430, 468
Hpy99I CGWCG 2 cut(s) 164, 407
HpyAV CCTTC 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 179, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 548
HpyF3I CTNAG 5 cut(s) 78, 312, 338, 380, 552
Hsp92II CATG 3 cut(s) 106, 188, 436
HspAI GCGC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 530
KpnI GGTACC 2 cut(s) 156, 510
KspAI GTTAAC 2 cut(s) 430, 468
Kzo9I GATC 2 cut(s) 125, 181
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 3 cut(s) 334, 461, 548
LpnPI CCDG 8 cut(s) 72, 240, 244, 367, 377, 438, 487, 544
LweI GCATC 3 cut(s) 166, 230, 521
MalI GATC 2 cut(s) 127, 183
MboI GATC 2 cut(s) 125, 181
MboII GAAGA 9 cut(s) 97, 284, 317, 354, 422, 425, 453, 457, 512
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 2 cut(s) 339, 349
MluCI AATT 3 cut(s) 42, 72, 359
MnlI CCTC 8 cut(s) 19, 112, 137, 271, 409, 415, 469, 546
MroI TCCGGA 1 cut(s) 530
MseI TTAA 2 cut(s) 429, 467
MslI CAYNNNNRTG 1 cut(s) 354
MspI CCGG 4 cut(s) 474, 531, 539, 546
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 185, 548
NdeII GATC 2 cut(s) 125, 181
NlaIII CATG 3 cut(s) 106, 188, 436
NlaIV GGNNCC 3 cut(s) 154, 457, 508
NmeAIII GCCGAG 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 308
PflFI GACNNNGTC 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 339
PspN4I GGNNCC 3 cut(s) 154, 457, 508
PspPI GGNCC 1 cut(s) 533
PsuI RGATCY 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 422
RsaI GTAC 2 cut(s) 154, 508
RsaNI GTAC 2 cut(s) 153, 507
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 2 cut(s) 429, 467
Sau3AI GATC 2 cut(s) 125, 181
Sau96I GGNCC 1 cut(s) 533
SduI GDGCHC 2 cut(s) 339, 349
SetI ASST 9 cut(s) 61, 158, 289, 339, 394, 426, 527, 538, 553
SfaNI GCATC 3 cut(s) 166, 230, 521
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 354
Sse9I AATT 3 cut(s) 42, 72, 359
SsiI CCGC 2 cut(s) 385, 510
SstI GAGCTC 1 cut(s) 339
TaqI TCGA 3 cut(s) 159, 402, 495
TasI AATT 3 cut(s) 42, 72, 359
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 429, 467
Tru9I TTAA 2 cut(s) 429, 467
TspDTI ATGAA 5 cut(s) 17, 318, 372, 423, 513
TspGWI ACGGA 1 cut(s) 125
Tth111I GACNNNGTC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 533
XagI CCTNNNNNAGG 1 cut(s) 141
XapI RAATTY 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.