Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007255

Physical Location & Seq

Genomic Coordinates Reverse (-)

30531699 .. 30535237

Gene Structure 3539 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1211900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGGCAATGGCCTCTACTTATTTTTCTACTTATTTGGTGGCACTGTTCTTCTTGGTTGTAGCTGGGTTTGATGCCTGCTCGGCTAGCCGGATTGGTTTGGGTTCAAGGCTGTTGGCTAGAGAGAACCAAACGCTGGTCTCAGATAATGGCACATTTGCTTTAGGGTTCACTCCAACGTCGGACGATGACTACAGACTTCAACTGGCTATTTGGTTTGCCGAGCTTCCCGGAGATCGAACCATAGTATGGTCTGCTAACAGGAACTCAGCCGTCTCCAACAATGCAATCCTAGAGCTTGATACAACCGGAAACCTTGTCCTCACGGATGGAGACGCTACCACGTGGACCTCAAACACCTCTGGCACTGGTGTTGAAGGCGCAACATTGCAAGAATCCGGAAATTTCGTTATCTACAATGATGTCAAGGGCCCGGTGTGGCAGAGTTTCTCACACCCATCTGACACTCTCCTCCCAAACCAACCTCTTTCAGTCTCTTTAGAGCTAACAACTTCTAAGTCCCCCTCACATGGTGGTTACTATGCACTCAAAATGCTTCAACAACGCACTTCTTTAAGCCTTGCCTTGACTTACAACGTGCCTGAAACTTTATACAATTCCTCCCCTGAATCATACTATAACTATTCTTATTGGAACGGACCAGACATATCAAATGTAACCGGGGATGTTGTAGCGGTTTTAGATGAAGCAGGAAGTTTTGGAATTGTGTATGGGGAGTCATCAGATGGAGCTGTTTATGTGTACAAGAATGATGGTGATAATGGGGGACTATCAGCAGCATCAAACATATCAACCAGATCATCGGTTCTTCGAAGATTGATTCTCGAGAGTAATGGGAATTTGCGCTTGTATCGTTGGGATAATGATGTTAACGGGTCAAGGCAATGGGTGCCAGAGTGGGCTGCAGTTTCAACACCATGTGACATCGCTGGAATTTGTGGCAATGGGATATGTAATTTGGATAGGAGTAAGACTAATGCTTCTTGCACATGTCTACCGGGGACTTATATGGTAGATGGGGGAAGTCAGTGTTCAGAAAATTCGTCACTTATTGGAAAATGTGATCCGCGGAGTAATTATCAGCCATCCCAGTTTAGAACTTCAACGGTGCAGCAAACCAATTACTATTTTCCAGAATTTTCAGTCATAGCAAACTACAGCGATGTTGATAATGTATCAAAATGTGGAGATATTTGTCTAACCGATTGTGAATGTGTTGCTTCAGTTTACAGCCTTGATGACGAAAGTCCATACTGTTGGGTTTTGAGAAGCTTGGGTTTTGGTGGCTATGAGGATCCTGGTTCCACTCTGTTTGTGAAGATTAGGTCTAATGGCTCAGAGGGCGATGCACAAGGATCCGGGGAGTCTTCGAAGGGGTCTGGTAATCGGCGAACAAAAGTTCTGGTTATTCCTATTGTTCTCAGCATGACCTTCCTTGTTGCCCTCCTATGTCTTTTGCTATATTACAATGTGCATAGGAGGAGATCCTTGAAGAGAACACTGGAAAGCTTGGTAATCTTGTCTGGTGCTCCGCTTAATCTTAGTTACCGCGAATTACAAATTCGTACGTGGAACTTCTCACAGCTTCTTGGAACTGGGGGATTTGGGAGTGTTTACAAGGGAAGCCTTGCAGATGGAACTTTGGTTGCCGTAAAAAAACTTGAAAGGGTTTTGCCACACGGGGAGAAGGAGTTTATAACCGAAGTGAACACCATTGGCTCCATGCATCACATGAACTTGGTTCGTCTATGCGGGTACTGCTCCGAGAGCTCACAACGGCTTTTAGTTTATGAGTTCATGAAAAATGGGTCACTGGACAAATGGATATTTGGCTCACGTTATTCCCGAGATAAGGTGCTAGATTGGCAATCACGCTTTGATATAGCCGTGGGTACTGCACAAGGAATTGCATATTTTCATGAGCAATGCCGAGATCGAATTATACATTGTGACATCAAACCAGAAAACATTCTGCTAGATGAAAATTTCTGTCCAAAAGTTTCCGATTTTGGACTAGCTAAGATGATGGGAAGAGAGCACTCACAGGTTGTTACCATGGTCAGAGGAACAAGAGGCTATTTGGCTCCGGAGTGGGTGAGTAACCGTCCTATAACCGTCAAGGCTGACGTTTATAGTTACGGTATGCTTCTTCTAGAGATCGTTGGCGGTCGAAGAAACCTGGACATGTCATTTGATGCAGATAACTTTTTCTATCCTGGATGGGCTTTTAAGGAGATGACAAGTGGAAATCCCATGAAAGTTGCAGACAGGAGACTCGAAGGAGCGGTGGAAGAAGAAGAACTCTTGAGAGCCTTGAAAGTTGCCTTTTGGTGCATCCAAGATGAGATATTCATGAGACCAACAATGGGGGAAGTGGTGAGAATGTTGGAAGGATCAGTCGGCATCAACATGCCACCAATGCCCCAAACGGTTTTGGAGTTAATTGAGGAAGGCCTAGATCATGTGTACAAGGCCATGAAGAGAGAATTCAATCAATCCAGCTCCTTCACCATCAATACCCATCCTTCTTCATCTCAAGCTACATGTAGTTACTCCACAATGTCCCCCAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin Pkinase S_locus_glycop

Protein Analysis

864

Amino Acids

95.39

Weight (kDa)

5.22

Isoelectric Point (pI)

39.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	80 - 167	2.7e-17	D-mannose binding lectin
S_locus_glycop	PF00954	258 - 340	2.7e-09	S-locus glycoprotein domain
Pkinase	PF00069	531 - 734	2.7e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	532 - 799	2.2e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24080 AT5G24080 AT5G24080
fragaria_vesca	FvH4_7g30670 FvH4_7g30670
malus_domestica	MD01G1211900.v1.1 MD07G1282400.v1.1
prunus_persica	Prupe.2G304500_v2.0.a1 Prupe.2G304500_v2.0.a1
pyrus_communis	pycom01g22230 pycom07g25630 pycom07g25640
rosa_chinensis	RchiOBHm_Chr1g0379601
rosa_laevigata	RLG00000026391
rosa_multiflora	Rmu_co8451749.1_g000001 Rmu_sc0011286.1_g000005
rosa_roxburghii	Rroxscaffold_4G00280050
rosa_rugosa	Rorug01G0413200 Rorug01G0413300 Rorug01G0413400
rosa_samantha	Rh1AG436400 Rh1BG393400 Rh1CG406200 Rh1DG424300
rosa_wichuraiana	Rw1G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1715
AccB1I	GGYRCC	1 cut(s)	907
AccB7I	CCANNNNNTGG	2 cut(s)	133, 246
AccBSI	CCGCTC	1 cut(s)	2303
AccI	GTMKAC	1 cut(s)	1012
AccII	CGCG	2 cut(s)	1087, 1569
AccIII	TCCGGA	2 cut(s)	395, 2104
AciI	CCGC	8 cut(s)	692, 1085, 1087, 1550, 1567, 1770, 2184, 2303
AclWI	GGATC	7 cut(s)	1076, 1307, 1320, 1368, 1381, 1497, 2419
AcsI	RAATTY	8 cut(s)	400, 856, 951, 1057, 1154, 1578, 2002, 2504
AcuI	CTGAAG	1 cut(s)	1224
AcvI	CACGTG	1 cut(s)	342
AdeI	CACNNNGTG	2 cut(s)	530, 938
AfaI	GTAC	5 cut(s)	761, 1585, 1775, 1912, 2486
AfiI	CCNNNNNNNGG	5 cut(s)	133, 246, 527, 1870, 2384
AflIII	ACRYGT	3 cut(s)	1007, 2202, 2561
AjnI	CCWGG	3 cut(s)	1315, 2196, 2233
Alw21I	GWGCWC	3 cut(s)	1549, 1790, 2058
Alw26I	GTCTC	6 cut(s)	142, 277, 324, 496, 2284, 2368
AlwI	GGATC	7 cut(s)	1076, 1307, 1320, 1368, 1381, 1497, 2419
Ama87I	CYCGRG	2 cut(s)	842, 1863
Aor13HI	TCCGGA	2 cut(s)	395, 2104
AoxI	GGCC	4 cut(s)	9, 427, 2470, 2490
ApaI	GGGCCC	1 cut(s)	431
ApeKI	GCWGC	3 cut(s)	794, 920, 1129
ApoI	RAATTY	8 cut(s)	400, 856, 951, 1057, 1154, 1578, 2002, 2504
ArsI	GACNNNNNNTTYG	2 cut(s)	2192, 2224
Asp700I	GAANNNNTTC	1 cut(s)	1986
AspLEI	GCGC	2 cut(s)	380, 864
AspS9I	GGNCC	4 cut(s)	345, 427, 428, 656
AsuC2I	CCSGG	5 cut(s)	228, 431, 679, 1017, 1378
AsuHPI	GGTGA	4 cut(s)	785, 2125, 2407, 2518
AsuII	TTCGAA	2 cut(s)	829, 1388
AsuNHI	GCTAGC	1 cut(s)	83
AvaI	CYCGRG	2 cut(s)	842, 1863
AvaII	GGWCC	2 cut(s)	345, 656
BaeGI	GKGCMC	1 cut(s)	431
BamHI	GGATCC	2 cut(s)	1312, 1373
BanI	GGYRCC	1 cut(s)	907
BanII	GRGCYC	2 cut(s)	431, 1790
BbrPI	CACGTG	1 cut(s)	342
BbsI	GAAGAC	1 cut(s)	1377
Bbv12I	GWGCWC	3 cut(s)	1549, 1790, 2058
BbvI	GCAGC	3 cut(s)	806, 907, 1141
BceAI	ACGGC	4 cut(s)	254, 1652, 1811, 1889
BciT130I	CCWGG	3 cut(s)	1317, 2198, 2235
BcnI	CCSGG	5 cut(s)	228, 431, 679, 1017, 1378
BcoDI	GTCTC	6 cut(s)	142, 277, 324, 496, 2284, 2368
BfaI	CTAG	8 cut(s)	84, 117, 290, 1877, 1994, 2033, 2171, 2474
BfmI	CTRYAG	3 cut(s)	190, 921, 1174
BglI	GCCNNNNNGGC	1 cut(s)	80
BisI	GCNGC	3 cut(s)	795, 921, 1130
BlsI	GCNGC	3 cut(s)	796, 922, 1131
Bme1390I	CCNGG	8 cut(s)	228, 431, 679, 1017, 1317, 1378, 2198, 2235
Bme18I	GGWCC	2 cut(s)	345, 656
BmeT110I	CYCGRG	2 cut(s)	842, 1863
BmgT120I	GGNCC	4 cut(s)	345, 427, 428, 656
BmiI	GGNNCC	7 cut(s)	429, 909, 1314, 1321, 1375, 1738, 2103
BmrFI	CCNGG	8 cut(s)	228, 431, 679, 1017, 1317, 1378, 2198, 2235
BmrI	ACTGGG	2 cut(s)	1102, 1623
BmsI	GCATC	7 cut(s)	61, 806, 1354, 1753, 2203, 2361, 2430
BmtI	GCTAGC	1 cut(s)	87
BmuI	ACTGGG	2 cut(s)	1102, 1623
BoxI	GACNNNNGTC	1 cut(s)	1263
BpiI	GAAGAC	1 cut(s)	1377
Bpu14I	TTCGAA	2 cut(s)	829, 1388
BpuEI	CTTGAG	2 cut(s)	2344, 2538
BpuMI	CCSGG	5 cut(s)	228, 431, 679, 1017, 1378
BsaAI	YACGTR	2 cut(s)	342, 1587
BsaI	GGTCTC	2 cut(s)	142, 2368
BsaJI	CCNNGG	6 cut(s)	678, 1016, 1085, 1377, 1905, 2073
BsaWI	WCCGGW	3 cut(s)	305, 395, 2104
BsaXI	ACNNNNNCTCC	4 cut(s)	602, 632, 1694, 1724
Bsc4I	CCNNNNNNNGG	5 cut(s)	133, 246, 527, 1870, 2384
Bse1I	ACTGG	6 cut(s)	207, 370, 1108, 1524, 1618, 1836
Bse3DI	GCAATG	5 cut(s)	12, 383, 908, 967, 1949
BseAI	TCCGGA	2 cut(s)	395, 2104
BseBI	CCWGG	3 cut(s)	1317, 2198, 2235
BseDI	CCNNGG	6 cut(s)	678, 1016, 1085, 1377, 1905, 2073
BseGI	GGATG	6 cut(s)	331, 688, 1103, 2243, 2352, 2539
BseLI	CCNNNNNNNGG	5 cut(s)	133, 246, 527, 1870, 2384
BseMI	GCAATG	5 cut(s)	12, 383, 908, 967, 1949
BseMII	CTCAG	4 cut(s)	153, 279, 1368, 1453
BseNI	ACTGG	6 cut(s)	207, 370, 1108, 1524, 1618, 1836
BseRI	GAGGAG	2 cut(s)	458, 1513
BseSI	GKGCMC	1 cut(s)	431
BseXI	GCAGC	3 cut(s)	806, 907, 1141
BseYI	CCCAGC	1 cut(s)	62
BsgI	GTGCAG	2 cut(s)	1148, 1899
Bsh1236I	CGCG	2 cut(s)	1087, 1569
Bsh1285I	CGRYCG	1 cut(s)	2188
BshFI	GGCC	4 cut(s)	11, 429, 2472, 2492
BshNI	GGYRCC	1 cut(s)	907
BsiEI	CGRYCG	1 cut(s)	2188
BsiHKAI	GWGCWC	3 cut(s)	1549, 1790, 2058
BsiHKCI	CYCGRG	2 cut(s)	842, 1863
BsiSI	CCGG	9 cut(s)	88, 228, 306, 396, 431, 678, 1016, 1377, 2105
BsiWI	CGTACG	1 cut(s)	1583
BslFI	GGGAC	4 cut(s)	502, 798, 1033, 2566
BslI	CCNNNNNNNGG	5 cut(s)	133, 246, 527, 1870, 2384
BsmAI	GTCTC	6 cut(s)	142, 277, 324, 496, 2284, 2368
BsmBI	CGTCTC	2 cut(s)	277, 324
BsmFI	GGGAC	4 cut(s)	502, 798, 1033, 2566
BsnI	GGCC	4 cut(s)	11, 429, 2472, 2492
Bso31I	GGTCTC	2 cut(s)	142, 2368
BsoBI	CYCGRG	2 cut(s)	842, 1863
Bsp119I	TTCGAA	2 cut(s)	829, 1388
Bsp120I	GGGCCC	1 cut(s)	427
Bsp1286I	GDGCHC	4 cut(s)	431, 1549, 1790, 2058
Bsp13I	TCCGGA	2 cut(s)	395, 2104
Bsp1407I	TGTACA	2 cut(s)	759, 2484
Bsp19I	CCATGG	1 cut(s)	2073
BspACI	CCGC	8 cut(s)	692, 1085, 1087, 1550, 1567, 1770, 2184, 2303
BspANI	GGCC	4 cut(s)	11, 429, 2472, 2492
BspCNI	CTCAG	4 cut(s)	152, 278, 1367, 1452
BspEI	TCCGGA	2 cut(s)	395, 2104
BspFNI	CGCG	2 cut(s)	1087, 1569
BspHI	TCATGA	3 cut(s)	1815, 1936, 2370
BspLI	GGNNCC	7 cut(s)	429, 909, 1314, 1321, 1375, 1738, 2103
BspMAI	CTGCAG	1 cut(s)	925
BspOI	GCTAGC	1 cut(s)	87
BspPI	GGATC	7 cut(s)	1076, 1307, 1320, 1368, 1381, 1497, 2419
BspT104I	TTCGAA	2 cut(s)	829, 1388
BspT107I	GGYRCC	1 cut(s)	907
BspTNI	GGTCTC	2 cut(s)	142, 2368
BsrBI	CCGCTC	1 cut(s)	2303
BsrDI	GCAATG	5 cut(s)	12, 383, 908, 967, 1949
BsrGI	TGTACA	2 cut(s)	759, 2484
BsrI	ACTGG	6 cut(s)	207, 370, 1108, 1524, 1618, 1836
BssECI	CCNNGG	6 cut(s)	678, 1016, 1085, 1377, 1905, 2073
BssT1I	CCWWGG	1 cut(s)	2073
Bst2UI	CCWGG	3 cut(s)	1317, 2198, 2235
Bst4CI	ACNGT	7 cut(s)	45, 1126, 1274, 2123, 2134, 2159, 2449
Bst6I	CTCTTC	3 cut(s)	1505, 2044, 2492
BstAPI	GCANNNNNTGC	1 cut(s)	907
BstAUI	TGTACA	2 cut(s)	759, 2484
BstBAI	YACGTR	2 cut(s)	342, 1587
BstBI	TTCGAA	2 cut(s)	829, 1388
BstC8I	GCNNGC	2 cut(s)	76, 85
BstDEI	CTNAG	7 cut(s)	139, 265, 513, 1354, 1439, 1559, 2037
BstDSI	CCRYGG	3 cut(s)	1085, 1905, 2073
BstF5I	GGATG	6 cut(s)	331, 688, 1103, 2243, 2352, 2539
BstFNI	CGCG	2 cut(s)	1087, 1569
BstHHI	GCGC	2 cut(s)	380, 864
BstMAI	GTCTC	6 cut(s)	142, 277, 324, 496, 2284, 2368
BstMCI	CGRYCG	1 cut(s)	2188
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 84, 907, 1359, 1776, 1785, 1882, 2437
BstNI	CCWGG	3 cut(s)	1317, 2198, 2235
BstNSI	RCATGY	4 cut(s)	1011, 2206, 2431, 2565
BstPAI	GACNNNNGTC	1 cut(s)	1263
BstSCI	CCNGG	8 cut(s)	226, 429, 677, 1015, 1315, 1376, 2196, 2233
BstSFI	CTRYAG	3 cut(s)	190, 921, 1174
BstSLI	GKGCMC	1 cut(s)	431
BstUI	CGCG	2 cut(s)	1087, 1569
BstV1I	GCAGC	3 cut(s)	806, 907, 1141
BstV2I	GAAGAC	1 cut(s)	1377
BstX2I	RGATCY	3 cut(s)	1312, 1373, 1502
BstXI	CCANNNNNNTGG	1 cut(s)	1275
BstYI	RGATCY	3 cut(s)	1312, 1373, 1502
BsuRI	GGCC	4 cut(s)	11, 429, 2472, 2492
BtgI	CCRYGG	3 cut(s)	1085, 1905, 2073
BtgZI	GCGATG	3 cut(s)	928, 1194, 1377
BtsCI	GGATG	6 cut(s)	331, 688, 1103, 2243, 2352, 2539
BtsIMutI	CAGTG	5 cut(s)	41, 363, 1052, 1517, 1829
Cac8I	GCNNGC	2 cut(s)	76, 85
CciI	TCATGA	3 cut(s)	1815, 1936, 2370
CfoI	GCGC	2 cut(s)	380, 864
Cfr13I	GGNCC	4 cut(s)	345, 427, 428, 656
Cfr42I	CCGCGG	1 cut(s)	1088
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	5 cut(s)	760, 1584, 1774, 1911, 2485
CspCI	CAANNNNNGTGG	2 cut(s)	1312, 1347
CviQI	GTAC	5 cut(s)	760, 1584, 1774, 1911, 2485
DdeI	CTNAG	7 cut(s)	139, 265, 513, 1354, 1439, 1559, 2037
DraIII	CACNNNGTG	2 cut(s)	530, 938
Eam1104I	CTCTTC	3 cut(s)	1505, 2044, 2492
EarI	CTCTTC	3 cut(s)	1505, 2044, 2492
Ecl136II	GAGCTC	1 cut(s)	1788
Eco130I	CCWWGG	1 cut(s)	2073
Eco147I	AGGCCT	1 cut(s)	2472
Eco24I	GRGCYC	2 cut(s)	431, 1790
Eco31I	GGTCTC	2 cut(s)	142, 2368
Eco47I	GGWCC	2 cut(s)	345, 656
Eco53kI	GAGCTC	1 cut(s)	1788
Eco57I	CTGAAG	1 cut(s)	1224
Eco72I	CACGTG	1 cut(s)	342
Eco88I	CYCGRG	2 cut(s)	842, 1863
EcoICRI	GAGCTC	1 cut(s)	1788
EcoO109I	RGGNCCY	1 cut(s)	427
EcoRI	GAATTC	1 cut(s)	2504
EcoRII	CCWGG	3 cut(s)	1315, 2196, 2233
EcoT14I	CCWWGG	1 cut(s)	2073
EcoT22I	ATGCAT	1 cut(s)	1746
EcoT38I	GRGCYC	2 cut(s)	431, 1790
ErhI	CCWWGG	1 cut(s)	2073
Esp3I	CGTCTC	2 cut(s)	277, 324
FaqI	GGGAC	4 cut(s)	502, 798, 1033, 2566
FauI	CCCGC	1 cut(s)	1763
FblI	GTMKAC	1 cut(s)	1012
Fnu4HI	GCNGC	3 cut(s)	795, 921, 1130
FokI	GGATG	6 cut(s)	338, 695, 1090, 2250, 2339, 2526
FriOI	GRGCYC	2 cut(s)	431, 1790
Fsp4HI	GCNGC	3 cut(s)	795, 921, 1130
FspBI	CTAG	8 cut(s)	84, 117, 290, 1877, 1994, 2033, 2171, 2474
GlaI	GCGC	2 cut(s)	379, 863
GluI	GCNGC	3 cut(s)	795, 921, 1130
GsaI	CCCAGC	1 cut(s)	66
HaeIII	GGCC	4 cut(s)	11, 429, 2472, 2492
HapII	CCGG	9 cut(s)	88, 228, 306, 396, 431, 678, 1016, 1377, 2105
HgaI	GACGC	1 cut(s)	341
HhaI	GCGC	2 cut(s)	380, 864
Hin6I	GCGC	2 cut(s)	378, 862
HinP1I	GCGC	2 cut(s)	378, 862
HincII	GTYRAC	1 cut(s)	889
HindII	GTYRAC	1 cut(s)	889
HindIII	AAGCTT	2 cut(s)	1288, 1525
HinfI	GANTC	6 cut(s)	392, 626, 734, 838, 1382, 2292
HpaI	GTTAAC	1 cut(s)	889
HpaII	CCGG	9 cut(s)	88, 228, 306, 396, 431, 678, 1016, 1377, 2105
HphI	GGTGA	4 cut(s)	785, 2125, 2407, 2518
Hpy166II	GTNNAC	9 cut(s)	168, 345, 760, 889, 1013, 1246, 1633, 1726, 2485
Hpy188I	TCNGA	9 cut(s)	142, 181, 460, 742, 1054, 1357, 1783, 2023, 2081
Hpy8I	GTNNAC	9 cut(s)	168, 345, 760, 889, 1013, 1246, 1633, 1726, 2485
Hpy99I	CGWCG	1 cut(s)	181
HpyAV	CCTTC	9 cut(s)	368, 1384, 1459, 1699, 2291, 2402, 2462, 2533, 2553
HpyCH4III	ACNGT	7 cut(s)	45, 1126, 1274, 2123, 2134, 2159, 2449
HpyCH4IV	ACGT	6 cut(s)	176, 341, 594, 1586, 1855, 2145
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 84, 907, 1359, 1776, 1785, 1882, 2437
HpyF3I	CTNAG	7 cut(s)	139, 265, 513, 1354, 1439, 1559, 2037
HpySE526I	ACGT	6 cut(s)	176, 341, 594, 1586, 1855, 2145
HspAI	GCGC	2 cut(s)	378, 862
Kpn2I	TCCGGA	2 cut(s)	395, 2104
KspAI	GTTAAC	1 cut(s)	889
KspI	CCGCGG	1 cut(s)	1088
LmnI	GCTCC	7 cut(s)	746, 1552, 1742, 1784, 2107, 2300, 2525
Lsp1109I	GCAGC	3 cut(s)	806, 907, 1141
LweI	GCATC	7 cut(s)	61, 806, 1354, 1753, 2203, 2361, 2430
MaeI	CTAG	8 cut(s)	84, 117, 290, 1877, 1994, 2033, 2171, 2474
MaeII	ACGT	6 cut(s)	176, 341, 594, 1586, 1855, 2145
MbiI	CCGCTC	1 cut(s)	2303
MflI	RGATCY	3 cut(s)	1312, 1373, 1502
MhlI	GDGCHC	4 cut(s)	431, 1549, 1790, 2058
MlyI	GAGTC	3 cut(s)	743, 1391, 2286
MmeI	TCCRAC	4 cut(s)	159, 197, 300, 2385
Mph1103I	ATGCAT	1 cut(s)	1746
MroI	TCCGGA	2 cut(s)	395, 2104
MroXI	GAANNNNTTC	1 cut(s)	1986
MseI	TTAA	5 cut(s)	572, 888, 1554, 2247, 2459
MslI	CAYNNNNRTG	1 cut(s)	2426
MspA1I	CMGCKG	1 cut(s)	1087
MspI	CCGG	9 cut(s)	88, 228, 306, 396, 431, 678, 1016, 1377, 2105
MspR9I	CCNGG	8 cut(s)	228, 431, 679, 1017, 1317, 1378, 2198, 2235
MvaI	CCWGG	3 cut(s)	1317, 2198, 2235
MvnI	CGCG	2 cut(s)	1087, 1569
MwoI	GCNNNNNNNGC	8 cut(s)	80, 84, 907, 1359, 1776, 1785, 1882, 2437
NciI	CCSGG	5 cut(s)	228, 431, 679, 1017, 1378
NcoI	CCATGG	1 cut(s)	2073
NheI	GCTAGC	1 cut(s)	83
NlaIV	GGNNCC	7 cut(s)	429, 909, 1314, 1321, 1375, 1738, 2103
NmeAIII	GCCGAG	3 cut(s)	59, 244, 1973
NmuCI	GTSAC	4 cut(s)	938, 1062, 1827, 1967
NsiI	ATGCAT	1 cut(s)	1746
NspI	RCATGY	4 cut(s)	1011, 2206, 2431, 2565
NspV	TTCGAA	2 cut(s)	829, 1388
PaeR7I	CTCGAG	1 cut(s)	842
PagI	TCATGA	3 cut(s)	1815, 1936, 2370
PceI	AGGCCT	1 cut(s)	2472
PciI	ACATGT	3 cut(s)	1007, 2202, 2561
PcsI	WCGNNNNNNNCGW	1 cut(s)	1861
PdmI	GAANNNNTTC	1 cut(s)	1986
PfeI	GAWTC	3 cut(s)	392, 626, 838
Pfl23II	CGTACG	1 cut(s)	1583
PflMI	CCANNNNNTGG	2 cut(s)	133, 246
PfoI	TCCNGGA	2 cut(s)	226, 2233
PkrI	GCNGC	3 cut(s)	796, 922, 1131
PleI	GAGTC	3 cut(s)	742, 1390, 2286
PmaCI	CACGTG	1 cut(s)	342
PmlI	CACGTG	1 cut(s)	342
PpsI	GAGTC	3 cut(s)	742, 1390, 2286
Ppu21I	YACGTR	2 cut(s)	342, 1587
PscI	ACATGT	3 cut(s)	1007, 2202, 2561
PshAI	GACNNNNGTC	1 cut(s)	1263
PsiI	TTATAA	1 cut(s)	1715
Psp124BI	GAGCTC	1 cut(s)	1790
Psp6I	CCWGG	3 cut(s)	1315, 2196, 2233
PspCI	CACGTG	1 cut(s)	342
PspFI	CCCAGC	1 cut(s)	62
PspGI	CCWGG	3 cut(s)	1315, 2196, 2233
PspLI	CGTACG	1 cut(s)	1583
PspN4I	GGNNCC	7 cut(s)	429, 909, 1314, 1321, 1375, 1738, 2103
PspOMI	GGGCCC	1 cut(s)	427
PspPI	GGNCC	4 cut(s)	345, 427, 428, 656
PstI	CTGCAG	1 cut(s)	925
PsuI	RGATCY	3 cut(s)	1312, 1373, 1502
RsaI	GTAC	5 cut(s)	761, 1585, 1775, 1912, 2486
RsaNI	GTAC	5 cut(s)	760, 1584, 1774, 1911, 2485
RseI	CAYNNNNRTG	1 cut(s)	2426
SacI	GAGCTC	1 cut(s)	1790
SacII	CCGCGG	1 cut(s)	1088
SaqAI	TTAA	5 cut(s)	572, 888, 1554, 2247, 2459
SatI	GCNGC	3 cut(s)	795, 921, 1130
Sau96I	GGNCC	4 cut(s)	345, 427, 428, 656
SchI	GAGTC	3 cut(s)	743, 1391, 2286
ScrFI	CCNGG	8 cut(s)	228, 431, 679, 1017, 1317, 1378, 2198, 2235
SduI	GDGCHC	4 cut(s)	431, 1549, 1790, 2058
SfaNI	GCATC	7 cut(s)	61, 806, 1354, 1753, 2203, 2361, 2430
SfcI	CTRYAG	3 cut(s)	190, 921, 1174
Sfr274I	CTCGAG	1 cut(s)	842
Sfr303I	CCGCGG	1 cut(s)	1088
SfuI	TTCGAA	2 cut(s)	829, 1388
SgrBI	CCGCGG	1 cut(s)	1088
SinI	GGWCC	2 cut(s)	345, 656
SlaI	CTCGAG	1 cut(s)	842
SmiMI	CAYNNNNRTG	1 cut(s)	2426
SmlI	CTYRAG	3 cut(s)	842, 2323, 2553
SmoI	CTYRAG	3 cut(s)	842, 2323, 2553
SseBI	AGGCCT	1 cut(s)	2472
SsiI	CCGC	8 cut(s)	692, 1085, 1087, 1550, 1567, 1770, 2184, 2303
SspMI	CTAG	8 cut(s)	84, 117, 290, 1877, 1994, 2033, 2171, 2474
SstI	GAGCTC	1 cut(s)	1790
StuI	AGGCCT	1 cut(s)	2472
StyD4I	CCNGG	8 cut(s)	226, 429, 677, 1015, 1315, 1376, 2196, 2233
StyI	CCWWGG	1 cut(s)	2073
TaaI	ACNGT	7 cut(s)	45, 1126, 1274, 2123, 2134, 2159, 2449
TaiI	ACGT	6 cut(s)	179, 344, 597, 1589, 1858, 2148
TaqI	TCGA	7 cut(s)	235, 829, 843, 1388, 1954, 2188, 2295
TatI	WGTACW	2 cut(s)	759, 2484
TfiI	GAWTC	3 cut(s)	392, 626, 838
Tru1I	TTAA	5 cut(s)	572, 888, 1554, 2247, 2459
Tru9I	TTAA	5 cut(s)	572, 888, 1554, 2247, 2459
TscAI	CASTG	5 cut(s)	48, 370, 1052, 1524, 1836
TseFI	GTSAC	4 cut(s)	938, 1062, 1827, 1967
TseI	GCWGC	3 cut(s)	794, 920, 1129
Tsp45I	GTSAC	4 cut(s)	938, 1062, 1827, 1967
TspGWI	ACGGA	2 cut(s)	338, 669
TspRI	CASTG	5 cut(s)	48, 370, 1052, 1524, 1836
Van91I	CCANNNNNTGG	2 cut(s)	133, 246
VpaK11BI	GGWCC	2 cut(s)	345, 656
XapI	RAATTY	8 cut(s)	400, 856, 951, 1057, 1154, 1578, 2002, 2504
XbaI	TCTAGA	1 cut(s)	2170
XceI	RCATGY	4 cut(s)	1011, 2206, 2431, 2565
XhoI	CTCGAG	1 cut(s)	842
XmiI	GTMKAC	1 cut(s)	1012
XmnI	GAANNNNTTC	1 cut(s)	1986
XspI	CTAG	8 cut(s)	84, 117, 290, 1877, 1994, 2033, 2171, 2474
Zsp2I	ATGCAT	1 cut(s)	1746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.