Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007255

Physical Location & Seq

Genomic Coordinates Forward (+)

34553136 .. 34556640

Gene Structure 3505 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1282400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCAATGTCCTCCACCTATTTTTCTACTTATTTGGTGGCACTGTTCTTCTTGGTTGTAGCTGGGTTTGATGCCTGCTCGGCTAGCCGGATTGGTTTGGGCTCGAGGCTGTTGGCTAGAGAGAACCAAACGTTGGTCTCAGATAATGGCACATTTGCTTTAGGGTTCACTCCAACGTCGGACGAAGACGACAGACTTCAACTGGCAATTTGGTTTGCGGAGCTTCCCGGAGATCGAACCATAGTATGGTCTGCTAACAGAAACTCAGCCGTCTCCAACAATGCGATCCTGGAGCTTGACACAACCGGAAACCTTGTCCTCATGGATGGAGACGCTACCACGTGGACCTCAAACACCTCCGGCGCTGGTGTTGAAGGCGCAACGTTGCAAGAATCTGGAAATTTCATTATCTATGACGATGTCAACCGCCCTGTGTGGCAGAGTTTCTCACACCCATCTGACACTCTCCTCCCAAACCAACCTCTTTCAGTCTCTTTAGAGCTAACAACTTCTAAGTCCTCCCCACGTGGTGGATACTATGCACTAAAAATGCTTCAACAACGCACTTCTTTAAGCCTTGCCTTGACTTACAACATGCCAGAAACTTTATACAATTCCTCCCCTGAATCATATTACAACTATTCTTATTGGAACGGACCAGACATATCAAATGTAACCGGGGATGTTGTAGCGGTTTTAGATGAAGCAGGGAGCTTTGGAATTGTGTATGGGGAGTCATCAGATGGAGCTGTATATGTGTACAAGAATGATGGCGATGATGGGGGACTATCAGCCGCATCAACCAGATCATTGGTTCTTCGTAGATTGATAATCGAGAGTAATGGGAATTTACGCTTGTATCGTTGGGATAATGATATTAATGGGTCAAGGCAATGGGTGCCAGAGTGGGCTGCAGTTTCAACACCATGTGACATTGCTGGAATTTGTGGTAAAGGGATATGTAATTTGGATAGGAGTAAGACTAATGCTTCTTGCACATGTCTACCGGGGACTTATAAGGAAAATGGGGGAAGTCTTTGTTCAGAAAATTCATCACTTACAGGAAAATGTGATTCGCGGAGTAATTATCAGCCATCCCAGTTTAGAATTTCAACGGTGCAGCAAACCAATTACTATTTTCCAGAATTTTCAGTCATAGCAAACTACAGCGATGTTGAGAATGTAACAAAATGTGGAGAGATTTGTCTAGCTGATTGTGAATGTGTTGCTTCAGTTTACGGCCTTGATGACGAAAATCCATACTGTTGGGTTTTGAGAAGCATGGATTTTGGTGGCTATGAGGATCCTGCTTCAACTCTGTTTGTGAAGGTTCGGTCTAATGGCTTAGAGGGCAACGCAAAAGGATCCGGGGAGTCTTCCAAGGGGTCGGGTAATCAGCAAACAAAAGTTTTGGTTATTCCTATTGTTCTTAGCATGACCTTCCTTGTTGCCCTCCTATGTCTTTTGCTATATTACAATGTCCATAGGAGGAGATCCTTGAAGAGAACACTGGAAAGCTCAGTAATCTTGTCTGGTGCTCCGTTGAATCTTAGTTACCGCGAATTACAAATTCGTACGTGGAATTTCTCACAGCTTCTTGGAACTGGGGGATTTGGGAGTGTTTACAAGGGAAGCCTTGCTGATGGCACTTTGGTTGCCGTAAAAAAACTTGAGAGGGTTTTGCCACATGGGGAGAAGGAGTTTATAACTGAAGTGAACACCATTGGTTCCATGCATCACATGAACTTGGTTCGTCTATGCGGGTACTGCTCCGAGAGCTCACAACGGCTTTTAGTTTATGAGTTCATGAAAAATGGGTCACTGGACAAATGGATATTTCGCTCAAATCATTCCCGAGACAAGTTGCTAGATTGGCAATCACGCTTTGATATAGCCGTGGGTACTGCACAAGGAATTGCATATTTTCATGAGCAATGCCGAGATCGAATTATACATTGTGACATCAAACCAGAAAACATTCTGCTGGATGAAAATTTCTGTCCTAAAGTTTCCGACTTTGGACTAGCTAAGTTGATGGGCAGAGAGCACTCACAGGTTGTTACCATGGTCAGAGGAACAAGAGGCTATTTGGCTCCGGAGTGGGTGAGTAACCGGCCTATAACTGTCAAGGCTGATGTTTATAGTTACGGTATGCTTCTTCTAGAGATCGTTGGTGGTCGAAGAAACCTTGACATGTCATTTGATGCAGATGACTTTTTCTATCCTGGATGGGCTTTTAAGGAGATGACAAGTGGAAATCCCATGAAAGTTGCAGACAGGAGACTGGAAGGAGCGGTTGAAGAAGAAGAACTCATTAGAGCCTTGAAAGTTGCCTTTTGGTGCATCCAAGATGAGATATTCATGAGACCAACAATGGGGGAAGTAGTGAGAATGTTGGAAGGATCAGTCGGCATCAACATGCCACCGATGCCCCAAACGGTTTTGGAGTTAATTGAGGAAGGCCTAGATCATGTGTACAAGGCAATGAAGAGAGAATTCAATCACTTCAGCTCCTTCACCATCAATACTCATCCTTCTTCATCTCAAGCTACATGCAGTTACTCCACAATGTCCCCTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin Pkinase S_locus_glycop

Protein Analysis

861

Amino Acids

95.39

Weight (kDa)

5.2

Isoelectric Point (pI)

38.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	80 - 167	2.7e-17	D-mannose binding lectin
S_locus_glycop	PF00954	257 - 338	1e-08	S-locus glycoprotein domain
Pkinase	PF00069	528 - 731	2.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	529 - 796	6.3e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24080 AT5G24080 AT5G24080
fragaria_vesca	FvH4_7g30670 FvH4_7g30670
malus_domestica	MD01G1211900.v1.1 MD07G1282400.v1.1
prunus_persica	Prupe.2G304500_v2.0.a1 Prupe.2G304500_v2.0.a1
pyrus_communis	pycom01g22230 pycom07g25630 pycom07g25640
rosa_chinensis	RchiOBHm_Chr1g0379601
rosa_laevigata	RLG00000026391
rosa_multiflora	Rmu_co8451749.1_g000001 Rmu_sc0011286.1_g000005
rosa_roxburghii	Rroxscaffold_4G00280050
rosa_rugosa	Rorug01G0413200 Rorug01G0413300 Rorug01G0413400
rosa_samantha	Rh1AG436400 Rh1BG393400 Rh1CG406200 Rh1DG424300
rosa_wichuraiana	Rw1G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1017, 1706
AccB1I	GGYRCC	1 cut(s)	898
AccB7I	CCANNNNNTGG	3 cut(s)	133, 246, 530
AccBSI	CCGCTC	1 cut(s)	2294
AccI	GTMKAC	1 cut(s)	1003
AccII	CGCG	2 cut(s)	1078, 1560
AccIII	TCCGGA	1 cut(s)	2095
AciI	CCGC	8 cut(s)	218, 427, 692, 795, 1078, 1558, 1761, 2294
AclI	AACGTT	2 cut(s)	131, 383
AclWI	GGATC	7 cut(s)	280, 1298, 1311, 1359, 1372, 1488, 2410
AcuI	CTGAAG	3 cut(s)	1215, 1731, 2491
AcvI	CACGTG	2 cut(s)	342, 527
AdeI	CACNNNGTG	2 cut(s)	530, 929
AfaI	GTAC	5 cut(s)	761, 1576, 1766, 1903, 2477
AfiI	CCNNNNNNNGG	4 cut(s)	133, 246, 530, 2375
AflIII	ACRYGT	2 cut(s)	998, 2193
AjnI	CCWGG	2 cut(s)	288, 2224
AjuI	GAANNNNNNNTTGG	2 cut(s)	116, 148
Alw21I	GWGCWC	3 cut(s)	1540, 1781, 2049
Alw26I	GTCTC	7 cut(s)	142, 277, 324, 496, 1851, 2275, 2359
AlwI	GGATC	7 cut(s)	280, 1298, 1311, 1359, 1372, 1488, 2410
Ama87I	CYCGRG	2 cut(s)	103, 1854
Aor13HI	TCCGGA	1 cut(s)	2095
AoxI	GGCC	3 cut(s)	1240, 2114, 2461
ApeKI	GCWGC	2 cut(s)	911, 1120
ArsI	GACNNNNNNTTYG	2 cut(s)	2183, 2215
AseI	ATTAAT	1 cut(s)	879
Asp700I	GAANNNNTTC	1 cut(s)	1977
AspLEI	GCGC	2 cut(s)	365, 380
AspS9I	GGNCC	2 cut(s)	345, 656
AsuC2I	CCSGG	4 cut(s)	228, 679, 1008, 1369
AsuHPI	GGTGA	2 cut(s)	2116, 2509
AsuNHI	GCTAGC	1 cut(s)	83
AvaI	CYCGRG	2 cut(s)	103, 1854
AvaII	GGWCC	2 cut(s)	345, 656
BaeI	ACNNNNGTAYC	2 cut(s)	842, 875
BamHI	GGATCC	2 cut(s)	1303, 1364
BanI	GGYRCC	1 cut(s)	898
BanII	GRGCYC	2 cut(s)	104, 1781
BbrPI	CACGTG	2 cut(s)	342, 527
BbsI	GAAGAC	2 cut(s)	192, 1368
Bbv12I	GWGCWC	3 cut(s)	1540, 1781, 2049
BbvI	GCAGC	2 cut(s)	898, 1132
BceAI	ACGGC	5 cut(s)	254, 1255, 1643, 1802, 1880
BciT130I	CCWGG	2 cut(s)	290, 2226
BciVI	GTATCC	1 cut(s)	527
BcnI	CCSGG	4 cut(s)	228, 679, 1008, 1369
BcoDI	GTCTC	7 cut(s)	142, 277, 324, 496, 1851, 2275, 2359
BfaI	CTAG	8 cut(s)	84, 117, 1208, 1868, 2024, 2162, 2465, 2577
BfmI	CTRYAG	2 cut(s)	912, 1165
BfoI	RGCGCY	1 cut(s)	366
BfuI	GTATCC	1 cut(s)	527
BglI	GCCNNNNNGGC	1 cut(s)	80
BisI	GCNGC	3 cut(s)	795, 912, 1121
BlsI	GCNGC	3 cut(s)	796, 913, 1122
Bme1390I	CCNGG	6 cut(s)	228, 290, 679, 1008, 1369, 2226
Bme18I	GGWCC	2 cut(s)	345, 656
BmeT110I	CYCGRG	2 cut(s)	103, 1854
BmgT120I	GGNCC	2 cut(s)	345, 656
BmiI	GGNNCC	5 cut(s)	900, 1305, 1366, 1729, 2094
BmrFI	CCNGG	6 cut(s)	228, 290, 679, 1008, 1369, 2226
BmrI	ACTGGG	2 cut(s)	1093, 1614
BmsI	GCATC	7 cut(s)	61, 806, 1744, 2194, 2352, 2418, 2421
BmtI	GCTAGC	1 cut(s)	87
BmuI	ACTGGG	2 cut(s)	1093, 1614
BpiI	GAAGAC	2 cut(s)	192, 1368
BpmI	CTGGAG	1 cut(s)	311
BpuEI	CTTGAG	2 cut(s)	1691, 2529
BpuMI	CCSGG	4 cut(s)	228, 679, 1008, 1369
BsaAI	YACGTR	3 cut(s)	342, 527, 1578
BsaI	GGTCTC	2 cut(s)	142, 2359
BsaJI	CCNNGG	6 cut(s)	678, 1007, 1368, 1380, 1896, 2064
BsaWI	WCCGGW	2 cut(s)	305, 2095
BsaXI	ACNNNNNCTCC	6 cut(s)	602, 632, 1685, 1715, 2495, 2525
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 246, 530, 2375
Bse118I	RCCGGY	1 cut(s)	2112
Bse1I	ACTGG	6 cut(s)	207, 1099, 1515, 1609, 1827, 2289
Bse3DI	GCAATG	5 cut(s)	12, 899, 933, 1940, 2490
BseAI	TCCGGA	1 cut(s)	2095
BseBI	CCWGG	2 cut(s)	290, 2226
BseDI	CCNNGG	6 cut(s)	678, 1007, 1368, 1380, 1896, 2064
BseGI	GGATG	7 cut(s)	331, 688, 1094, 1993, 2234, 2343, 2530
BseLI	CCNNNNNNNGG	4 cut(s)	133, 246, 530, 2375
BseMI	GCAATG	5 cut(s)	12, 899, 933, 1940, 2490
BseMII	CTCAG	3 cut(s)	153, 279, 1533
BseNI	ACTGG	6 cut(s)	207, 1099, 1515, 1609, 1827, 2289
BseRI	GAGGAG	2 cut(s)	458, 1504
BseXI	GCAGC	2 cut(s)	898, 1132
BseYI	CCCAGC	1 cut(s)	62
BsgI	GTGCAG	2 cut(s)	1139, 1890
Bsh1236I	CGCG	2 cut(s)	1078, 1560
BshFI	GGCC	3 cut(s)	1242, 2116, 2463
BshNI	GGYRCC	1 cut(s)	898
BsiHKAI	GWGCWC	3 cut(s)	1540, 1781, 2049
BsiHKCI	CYCGRG	2 cut(s)	103, 1854
BsiSI	CCGG	9 cut(s)	88, 228, 306, 360, 678, 1007, 1368, 2096, 2113
BsiWI	CGTACG	1 cut(s)	1574
BslFI	GGGAC	3 cut(s)	798, 1024, 2557
BslI	CCNNNNNNNGG	4 cut(s)	133, 246, 530, 2375
BsmAI	GTCTC	7 cut(s)	142, 277, 324, 496, 1851, 2275, 2359
BsmBI	CGTCTC	2 cut(s)	277, 324
BsmFI	GGGAC	3 cut(s)	798, 1024, 2557
BsnI	GGCC	3 cut(s)	1242, 2116, 2463
Bso31I	GGTCTC	2 cut(s)	142, 2359
BsoBI	CYCGRG	2 cut(s)	103, 1854
Bsp1286I	GDGCHC	4 cut(s)	104, 1540, 1781, 2049
Bsp13I	TCCGGA	1 cut(s)	2095
Bsp1407I	TGTACA	2 cut(s)	759, 2475
Bsp19I	CCATGG	1 cut(s)	2064
BspACI	CCGC	8 cut(s)	218, 427, 692, 795, 1078, 1558, 1761, 2294
BspANI	GGCC	3 cut(s)	1242, 2116, 2463
BspCNI	CTCAG	3 cut(s)	152, 278, 1532
BspEI	TCCGGA	1 cut(s)	2095
BspFNI	CGCG	2 cut(s)	1078, 1560
BspHI	TCATGA	3 cut(s)	1806, 1927, 2361
BspLI	GGNNCC	5 cut(s)	900, 1305, 1366, 1729, 2094
BspMAI	CTGCAG	1 cut(s)	916
BspOI	GCTAGC	1 cut(s)	87
BspPI	GGATC	7 cut(s)	280, 1298, 1311, 1359, 1372, 1488, 2410
BspT107I	GGYRCC	1 cut(s)	898
BspTNI	GGTCTC	2 cut(s)	142, 2359
BsrBI	CCGCTC	1 cut(s)	2294
BsrDI	GCAATG	5 cut(s)	12, 899, 933, 1940, 2490
BsrFI	RCCGGY	1 cut(s)	2112
BsrGI	TGTACA	2 cut(s)	759, 2475
BsrI	ACTGG	6 cut(s)	207, 1099, 1515, 1609, 1827, 2289
BssAI	RCCGGY	1 cut(s)	2112
BssECI	CCNNGG	6 cut(s)	678, 1007, 1368, 1380, 1896, 2064
BssT1I	CCWWGG	2 cut(s)	1380, 2064
Bst2UI	CCWGG	2 cut(s)	290, 2226
Bst4CI	ACNGT	6 cut(s)	45, 1117, 1265, 2125, 2150, 2440
Bst6I	CTCTTC	2 cut(s)	1496, 2483
BstAPI	GCANNNNNTGC	1 cut(s)	898
BstAUI	TGTACA	2 cut(s)	759, 2475
BstBAI	YACGTR	3 cut(s)	342, 527, 1578
BstC8I	GCNNGC	2 cut(s)	76, 85
BstDEI	CTNAG	8 cut(s)	139, 265, 513, 1345, 1430, 1519, 1550, 2028
BstDSI	CCRYGG	2 cut(s)	1896, 2064
BstF5I	GGATG	7 cut(s)	331, 688, 1094, 1993, 2234, 2343, 2530
BstFNI	CGCG	2 cut(s)	1078, 1560
BstH2I	RGCGCY	1 cut(s)	366
BstHHI	GCGC	2 cut(s)	365, 380
BstMAI	GTCTC	7 cut(s)	142, 277, 324, 496, 1851, 2275, 2359
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 84, 898, 1350, 1767, 1776, 1873, 2428
BstNI	CCWGG	2 cut(s)	290, 2226
BstNSI	RCATGY	5 cut(s)	598, 1002, 2197, 2422, 2556
BstSCI	CCNGG	6 cut(s)	226, 288, 677, 1006, 1367, 2224
BstSFI	CTRYAG	2 cut(s)	912, 1165
BstUI	CGCG	2 cut(s)	1078, 1560
BstV1I	GCAGC	2 cut(s)	898, 1132
BstV2I	GAAGAC	2 cut(s)	192, 1368
BstX2I	RGATCY	3 cut(s)	1303, 1364, 1493
BstXI	CCANNNNNNTGG	2 cut(s)	811, 1266
BstYI	RGATCY	3 cut(s)	1303, 1364, 1493
BsuI	GTATCC	1 cut(s)	527
BsuRI	GGCC	3 cut(s)	1242, 2116, 2463
BtgI	CCRYGG	2 cut(s)	1896, 2064
BtgZI	GCGATG	2 cut(s)	789, 1185
BtsCI	GGATG	7 cut(s)	331, 688, 1094, 1993, 2234, 2343, 2530
BtsIMutI	CAGTG	3 cut(s)	41, 1508, 1820
Cac8I	GCNNGC	2 cut(s)	76, 85
CciI	TCATGA	3 cut(s)	1806, 1927, 2361
CfoI	GCGC	2 cut(s)	365, 380
Cfr10I	RCCGGY	1 cut(s)	2112
Cfr13I	GGNCC	2 cut(s)	345, 656
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	5 cut(s)	760, 1575, 1765, 1902, 2476
CviQI	GTAC	5 cut(s)	760, 1575, 1765, 1902, 2476
DdeI	CTNAG	8 cut(s)	139, 265, 513, 1345, 1430, 1519, 1550, 2028
DraIII	CACNNNGTG	2 cut(s)	530, 929
Eam1104I	CTCTTC	2 cut(s)	1496, 2483
EarI	CTCTTC	2 cut(s)	1496, 2483
Ecl136II	GAGCTC	1 cut(s)	1779
Eco130I	CCWWGG	2 cut(s)	1380, 2064
Eco147I	AGGCCT	1 cut(s)	2463
Eco24I	GRGCYC	2 cut(s)	104, 1781
Eco31I	GGTCTC	2 cut(s)	142, 2359
Eco47I	GGWCC	2 cut(s)	345, 656
Eco53kI	GAGCTC	1 cut(s)	1779
Eco57I	CTGAAG	3 cut(s)	1215, 1731, 2491
Eco72I	CACGTG	2 cut(s)	342, 527
Eco88I	CYCGRG	2 cut(s)	103, 1854
EcoICRI	GAGCTC	1 cut(s)	1779
EcoRI	GAATTC	1 cut(s)	2495
EcoRII	CCWGG	2 cut(s)	288, 2224
EcoT14I	CCWWGG	2 cut(s)	1380, 2064
EcoT22I	ATGCAT	1 cut(s)	1737
EcoT38I	GRGCYC	2 cut(s)	104, 1781
ErhI	CCWWGG	2 cut(s)	1380, 2064
Esp3I	CGTCTC	2 cut(s)	277, 324
FaqI	GGGAC	3 cut(s)	798, 1024, 2557
FauI	CCCGC	1 cut(s)	1754
FblI	GTMKAC	1 cut(s)	1003
Fnu4HI	GCNGC	3 cut(s)	795, 912, 1121
FokI	GGATG	7 cut(s)	338, 695, 1081, 2000, 2241, 2330, 2517
FriOI	GRGCYC	2 cut(s)	104, 1781
Fsp4HI	GCNGC	3 cut(s)	795, 912, 1121
FspBI	CTAG	8 cut(s)	84, 117, 1208, 1868, 2024, 2162, 2465, 2577
GlaI	GCGC	2 cut(s)	364, 379
GluI	GCNGC	3 cut(s)	795, 912, 1121
GsaI	CCCAGC	1 cut(s)	66
GsuI	CTGGAG	1 cut(s)	311
HaeII	RGCGCY	1 cut(s)	366
HaeIII	GGCC	3 cut(s)	1242, 2116, 2463
HapII	CCGG	9 cut(s)	88, 228, 306, 360, 678, 1007, 1368, 2096, 2113
HgaI	GACGC	1 cut(s)	341
HhaI	GCGC	2 cut(s)	365, 380
Hin6I	GCGC	2 cut(s)	363, 378
HinP1I	GCGC	2 cut(s)	363, 378
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HinfI	GANTC	6 cut(s)	392, 626, 734, 1073, 1373, 1546
HpaII	CCGG	9 cut(s)	88, 228, 306, 360, 678, 1007, 1368, 2096, 2113
HphI	GGTGA	2 cut(s)	2116, 2509
Hpy166II	GTNNAC	9 cut(s)	168, 345, 424, 760, 1004, 1237, 1624, 1717, 2476
Hpy188I	TCNGA	8 cut(s)	142, 181, 460, 742, 1045, 1774, 2014, 2072
Hpy188III	TCNNGA	9 cut(s)	396, 835, 1142, 1807, 1854, 1928, 2096, 2162, 2362
Hpy8I	GTNNAC	9 cut(s)	168, 345, 424, 760, 1004, 1237, 1624, 1717, 2476
Hpy99I	CGWCG	1 cut(s)	181
HpyAV	CCTTC	9 cut(s)	368, 1321, 1450, 1690, 2282, 2393, 2453, 2524, 2544
HpyCH4III	ACNGT	6 cut(s)	45, 1117, 1265, 2125, 2150, 2440
HpyCH4IV	ACGT	6 cut(s)	131, 176, 341, 383, 526, 1577
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 84, 898, 1350, 1767, 1776, 1873, 2428
HpyF3I	CTNAG	8 cut(s)	139, 265, 513, 1345, 1430, 1519, 1550, 2028
HpySE526I	ACGT	6 cut(s)	131, 176, 341, 383, 526, 1577
HspAI	GCGC	2 cut(s)	363, 378
Kpn2I	TCCGGA	1 cut(s)	2095
LmnI	GCTCC	9 cut(s)	220, 292, 711, 746, 1543, 1775, 2098, 2291, 2516
Lsp1109I	GCAGC	2 cut(s)	898, 1132
LweI	GCATC	7 cut(s)	61, 806, 1744, 2194, 2352, 2418, 2421
MaeI	CTAG	8 cut(s)	84, 117, 1208, 1868, 2024, 2162, 2465, 2577
MaeII	ACGT	6 cut(s)	131, 176, 341, 383, 526, 1577
MbiI	CCGCTC	1 cut(s)	2294
MflI	RGATCY	3 cut(s)	1303, 1364, 1493
MhlI	GDGCHC	4 cut(s)	104, 1540, 1781, 2049
MlyI	GAGTC	2 cut(s)	743, 1382
MmeI	TCCRAC	5 cut(s)	159, 197, 300, 2037, 2376
Mph1103I	ATGCAT	1 cut(s)	1737
MroI	TCCGGA	1 cut(s)	2095
MroXI	GAANNNNTTC	1 cut(s)	1977
MseI	TTAA	4 cut(s)	572, 879, 2238, 2450
MslI	CAYNNNNRTG	1 cut(s)	2417
MspI	CCGG	9 cut(s)	88, 228, 306, 360, 678, 1007, 1368, 2096, 2113
MspR9I	CCNGG	6 cut(s)	228, 290, 679, 1008, 1369, 2226
MvaI	CCWGG	2 cut(s)	290, 2226
MvnI	CGCG	2 cut(s)	1078, 1560
MwoI	GCNNNNNNNGC	8 cut(s)	80, 84, 898, 1350, 1767, 1776, 1873, 2428
NciI	CCSGG	4 cut(s)	228, 679, 1008, 1369
NcoI	CCATGG	1 cut(s)	2064
NheI	GCTAGC	1 cut(s)	83
NlaIV	GGNNCC	5 cut(s)	900, 1305, 1366, 1729, 2094
NmeAIII	GCCGAG	2 cut(s)	59, 1964
NmuCI	GTSAC	3 cut(s)	929, 1818, 1958
NsiI	ATGCAT	1 cut(s)	1737
NspI	RCATGY	5 cut(s)	598, 1002, 2197, 2422, 2556
PaeR7I	CTCGAG	1 cut(s)	103
PagI	TCATGA	3 cut(s)	1806, 1927, 2361
PceI	AGGCCT	1 cut(s)	2463
PciI	ACATGT	2 cut(s)	998, 2193
PcsI	WCGNNNNNNNCGW	1 cut(s)	859
PdmI	GAANNNNTTC	1 cut(s)	1977
PfeI	GAWTC	4 cut(s)	392, 626, 1073, 1546
Pfl23II	CGTACG	1 cut(s)	1574
PflFI	GACNNNGTC	1 cut(s)	419
PflMI	CCANNNNNTGG	3 cut(s)	133, 246, 530
PfoI	TCCNGGA	3 cut(s)	226, 288, 2224
PkrI	GCNGC	3 cut(s)	796, 913, 1122
PleI	GAGTC	2 cut(s)	742, 1381
PmaCI	CACGTG	2 cut(s)	342, 527
PmlI	CACGTG	2 cut(s)	342, 527
PpsI	GAGTC	2 cut(s)	742, 1381
Ppu21I	YACGTR	3 cut(s)	342, 527, 1578
PscI	ACATGT	2 cut(s)	998, 2193
PshBI	ATTAAT	1 cut(s)	879
PsiI	TTATAA	2 cut(s)	1017, 1706
Psp124BI	GAGCTC	1 cut(s)	1781
Psp1406I	AACGTT	2 cut(s)	131, 383
Psp6I	CCWGG	2 cut(s)	288, 2224
PspCI	CACGTG	2 cut(s)	342, 527
PspFI	CCCAGC	1 cut(s)	62
PspGI	CCWGG	2 cut(s)	288, 2224
PspLI	CGTACG	1 cut(s)	1574
PspN4I	GGNNCC	5 cut(s)	900, 1305, 1366, 1729, 2094
PspPI	GGNCC	2 cut(s)	345, 656
PspXI	VCTCGAGB	1 cut(s)	103
PstI	CTGCAG	1 cut(s)	916
PsuI	RGATCY	3 cut(s)	1303, 1364, 1493
PsyI	GACNNNGTC	1 cut(s)	419
RsaI	GTAC	5 cut(s)	761, 1576, 1766, 1903, 2477
RsaNI	GTAC	5 cut(s)	760, 1575, 1765, 1902, 2476
RseI	CAYNNNNRTG	1 cut(s)	2417
SacI	GAGCTC	1 cut(s)	1781
SaqAI	TTAA	4 cut(s)	572, 879, 2238, 2450
SatI	GCNGC	3 cut(s)	795, 912, 1121
Sau96I	GGNCC	2 cut(s)	345, 656
SchI	GAGTC	2 cut(s)	743, 1382
ScrFI	CCNGG	6 cut(s)	228, 290, 679, 1008, 1369, 2226
SduI	GDGCHC	4 cut(s)	104, 1540, 1781, 2049
SfaNI	GCATC	7 cut(s)	61, 806, 1744, 2194, 2352, 2418, 2421
SfcI	CTRYAG	2 cut(s)	912, 1165
Sfr274I	CTCGAG	1 cut(s)	103
SinI	GGWCC	2 cut(s)	345, 656
SlaI	CTCGAG	1 cut(s)	103
SmiMI	CAYNNNNRTG	1 cut(s)	2417
SmlI	CTYRAG	3 cut(s)	103, 1670, 2544
SmoI	CTYRAG	3 cut(s)	103, 1670, 2544
SseBI	AGGCCT	1 cut(s)	2463
SsiI	CCGC	8 cut(s)	218, 427, 692, 795, 1078, 1558, 1761, 2294
SspMI	CTAG	8 cut(s)	84, 117, 1208, 1868, 2024, 2162, 2465, 2577
SstI	GAGCTC	1 cut(s)	1781
StuI	AGGCCT	1 cut(s)	2463
StyD4I	CCNGG	6 cut(s)	226, 288, 677, 1006, 1367, 2224
StyI	CCWWGG	2 cut(s)	1380, 2064
TaaI	ACNGT	6 cut(s)	45, 1117, 1265, 2125, 2150, 2440
TaiI	ACGT	6 cut(s)	134, 179, 344, 386, 529, 1580
TaqI	TCGA	5 cut(s)	104, 235, 834, 1945, 2179
TaqII	GACCGA	1 cut(s)	1323
TatI	WGTACW	2 cut(s)	759, 2475
TauI	GCSGC	1 cut(s)	797
TfiI	GAWTC	4 cut(s)	392, 626, 1073, 1546
Tru1I	TTAA	4 cut(s)	572, 879, 2238, 2450
Tru9I	TTAA	4 cut(s)	572, 879, 2238, 2450
TscAI	CASTG	3 cut(s)	48, 1515, 1827
TseFI	GTSAC	3 cut(s)	929, 1818, 1958
TseI	GCWGC	2 cut(s)	911, 1120
Tsp45I	GTSAC	3 cut(s)	929, 1818, 1958
TspGWI	ACGGA	2 cut(s)	669, 1530
TspRI	CASTG	3 cut(s)	48, 1515, 1827
Tth111I	GACNNNGTC	1 cut(s)	419
Van91I	CCANNNNNTGG	3 cut(s)	133, 246, 530
VpaK11BI	GGWCC	2 cut(s)	345, 656
VspI	ATTAAT	1 cut(s)	879
XbaI	TCTAGA	1 cut(s)	2161
XceI	RCATGY	5 cut(s)	598, 1002, 2197, 2422, 2556
XhoI	CTCGAG	1 cut(s)	103
XmiI	GTMKAC	1 cut(s)	1003
XmnI	GAANNNNTTC	1 cut(s)	1977
XspI	CTAG	8 cut(s)	84, 117, 1208, 1868, 2024, 2162, 2465, 2577
Zsp2I	ATGCAT	1 cut(s)	1737

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.