MD01G1219300.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008674
Physical Location & Seq
Reverse (-)
31159994 .. 31162992
2999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1219300.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGAATATTCCCAGACCAATCTTGTTCTTCTTCATCCTCTTCTTCTTCTTCTTGTCAAGCAGCTTAGAAAATTGCATGTTGGATGATATCTGCGGCGACGGCGAGCCGGCCATCCAATTCCCGTTTGGGATACAAAAAAACCAGCCAGAGTCGTGCTCCTATCCCGGTTTTGAACTGTCCTGTGACAGCAGCAACCATACAATCCTCACCCTTCCTTATTCCGGGCAGTTCGCTGTCCAGGGTATAGACTATGCCGAACAACAAATATGGATTAACGATCCAAACAGCTGCCTCGCACAACGAGTTTTGTCCCTCAATCTCTCAGGCTCTCCATTTTATGGATTGACAAACGAAAGCTTTACGTTCTTTAACTGCTCGTTGGATTATTTGAAATACAGATTATTAAACCCTATTGCTTGCATGAGCGGCTCTACGTACACAGTTTTTGCCACCACTTCTTCGAGGGTGATTAGTTTCTTGGCGTCTACATCGACATGCACTCGAATCGGTACGTTTGTTGTTCCGGTTGAGTTTCCGTTGTACGCGGAGGTTATGTCGTCGGACCTGAATGGCCATCTCCGGCTCGCATGGGACTGGCCAGGGTGCGGCCGTTGTGCCGCACGTGGTGGCCGGTGTGGGTTTGAGAGTAATTCTAGTCATCAAGTTGTTTGCTCAAGACTTCCTCAGCGAGCTGTTGGACGTGAGATGCCAGCAATCTTTGCTATTTGGGTTTTATGCTGCGTCTTTGGCAAGGTCAAGTCGTTAACAAGGGGACATAGAACTCTGCCTGAGTTTAATTCCGCAGTGGCTCCGCAGCCCACCATCGTCAGGGGTCTCGATAGGCCGAGCCTCGAGGCCTACCCAAAAATAGTCCTTGGAGAAAGCCGGCGCTTACCAAAGCCCGACGACAACATGTGCTCAATATGCTTGTCCGAGTACCGGCCCAAGGAGACATTGAAGACCATCCCCCCATGCCAGCATTGCTTCCATGCTGATTGCATCGATGAATGGCTTCAAATGAATGCTACTTGCCCTATTTGTCGGAATTCTCCACCGTGA

Protein Analysis

353

Amino Acids

39.27

Weight (kDa)

6.31

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 93 4.5e-19 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 304 - 348 4.9e-14 Ring finger domain
zf-C3HC4_2 PF13923 305 - 347 2.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 306 - 334 3.9e-09 RING-like zinc finger
zf-rbx1 PF12678 306 - 348 1.7e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 306 - 347 2.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 306 - 345 1.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 851
AccB7I CCANNNNNTGG 1 cut(s) 338
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 485
AccII CGCG 1 cut(s) 545
AciI CCGC 7 cut(s) 93, 426, 545, 606, 618, 801, 812
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 5 cut(s) 108, 571, 596, 607, 628
AcsI RAATTY 1 cut(s) 1045
AcvI CACGTG 1 cut(s) 623
AcyI GRCGYC 1 cut(s) 482
AdeI CACNNNGTG 1 cut(s) 626
AfaI GTAC 4 cut(s) 437, 511, 542, 938
AfiI CCNNNNNNNGG 6 cut(s) 221, 338, 605, 828, 939, 946
AflIII ACRYGT 1 cut(s) 912
AgsI TTSAA 4 cut(s) 173, 391, 958, 1016
AjiI CACGTC 1 cut(s) 701
AjnI CCWGG 2 cut(s) 237, 598
AluBI AGCT 4 cut(s) 63, 288, 357, 692
AluI AGCT 4 cut(s) 63, 288, 357, 692
Alw21I GWGCWC 2 cut(s) 158, 920
Alw26I GTCTC 2 cut(s) 839, 944
AlwI GGATC 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 851
AoxI GGCC 8 cut(s) 108, 571, 596, 607, 628, 842, 855, 941
ApeKI GCWGC 5 cut(s) 60, 189, 288, 738, 814
ApoI RAATTY 1 cut(s) 1045
Asp700I GAANNNNTTC 1 cut(s) 1011
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 2 cut(s) 562, 942
AsuC2I CCSGG 2 cut(s) 165, 223
AsuHPI GGTGA 2 cut(s) 199, 478
AvaI CYCGRG 1 cut(s) 851
AvaII GGWCC 1 cut(s) 562
BaeI ACNNNNGTAYC 2 cut(s) 501, 534
BalI TGGCCA 2 cut(s) 573, 598
BbrPI CACGTG 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 2 cut(s) 158, 920
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 5 cut(s) 72, 201, 275, 725, 826
BccI CCATC 4 cut(s) 119, 582, 830, 971
BceAI ACGGC 2 cut(s) 115, 594
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 2 cut(s) 239, 600
BciVI GTATCC 1 cut(s) 123
BcnI CCSGG 2 cut(s) 165, 223
BcoDI GTCTC 2 cut(s) 839, 944
BfaI CTAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 892
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 9 cut(s) 61, 94, 190, 289, 427, 607, 618, 739, 815
BlsI GCNGC 9 cut(s) 62, 95, 191, 290, 428, 608, 619, 740, 816
Bme1390I CCNGG 4 cut(s) 165, 223, 239, 600
Bme18I GGWCC 1 cut(s) 562
BmeT110I CYCGRG 1 cut(s) 851
BmgBI CACGTC 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 562, 942
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 4 cut(s) 165, 223, 239, 600
BmsI GCATC 2 cut(s) 696, 1008
BpiI GAAGAC 1 cut(s) 965
BplI GAGNNNNNCTC 2 cut(s) 140, 172
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 658
BpuMI CCSGG 2 cut(s) 165, 223
Bsa29I ATCGAT 1 cut(s) 1002
BsaAI YACGTR 2 cut(s) 435, 623
BsaHI GRCGYC 1 cut(s) 482
BsaI GGTCTC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 238, 599, 874, 945
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 6 cut(s) 221, 338, 605, 828, 939, 946
Bse118I RCCGGY 4 cut(s) 106, 630, 885, 939
Bse1I ACTGG 1 cut(s) 599
Bse3DI GCAATG 1 cut(s) 979
BseBI CCWGG 2 cut(s) 239, 600
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 4 cut(s) 238, 599, 874, 945
BseGI GGATG 4 cut(s) 33, 88, 111, 963
BseLI CCNNNNNNNGG 6 cut(s) 221, 338, 605, 828, 939, 946
BseMI GCAATG 1 cut(s) 979
BseMII CTCAG 3 cut(s) 336, 698, 780
BseNI ACTGG 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 607
BseXI GCAGC 5 cut(s) 72, 201, 275, 725, 826
Bsh1236I CGCG 1 cut(s) 545
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 8 cut(s) 110, 573, 598, 609, 630, 844, 857, 943
BshVI ATCGAT 1 cut(s) 1002
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 2 cut(s) 158, 920
BsiHKCI CYCGRG 1 cut(s) 851
BsiSI CCGG 8 cut(s) 107, 165, 222, 524, 580, 631, 886, 940
BslFI GGGAC 3 cut(s) 295, 605, 786
BslI CCNNNNNNNGG 6 cut(s) 221, 338, 605, 828, 939, 946
BsmAI GTCTC 2 cut(s) 839, 944
BsmFI GGGAC 3 cut(s) 295, 605, 786
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 8 cut(s) 110, 573, 598, 609, 630, 844, 857, 943
Bso31I GGTCTC 1 cut(s) 839
BsoBI CYCGRG 1 cut(s) 851
Bsp1286I GDGCHC 2 cut(s) 158, 920
Bsp143I GATC 1 cut(s) 277
BspACI CCGC 7 cut(s) 93, 426, 545, 606, 618, 801, 812
BspANI GGCC 8 cut(s) 110, 573, 598, 609, 630, 844, 857, 943
BspCNI CTCAG 3 cut(s) 335, 697, 781
BspDI ATCGAT 1 cut(s) 1002
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 810
BspPI GGATC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 839
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 979
BsrFI RCCGGY 4 cut(s) 106, 630, 885, 939
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 4 cut(s) 106, 630, 885, 939
BssECI CCNNGG 4 cut(s) 238, 599, 874, 945
BssMI GATC 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 482
BssT1I CCWWGG 2 cut(s) 874, 945
Bst2UI CCWGG 2 cut(s) 239, 600
Bst4CI ACNGT 3 cut(s) 177, 442, 1056
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 2 cut(s) 435, 623
BstC8I GCNNGC 8 cut(s) 104, 108, 418, 585, 690, 711, 887, 977
BstDEI CTNAG 4 cut(s) 64, 322, 684, 789
BstF5I GGATG 4 cut(s) 33, 88, 111, 963
BstFNI CGCG 1 cut(s) 545
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 2 cut(s) 839, 944
BstMBI GATC 1 cut(s) 277
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 6 cut(s) 99, 426, 719, 747, 924, 981
BstNI CCWGG 2 cut(s) 239, 600
BstNSI RCATGY 3 cut(s) 79, 498, 916
BstSCI CCNGG 4 cut(s) 163, 221, 237, 598
BstSNI TACGTA 1 cut(s) 435
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 5 cut(s) 72, 201, 275, 725, 826
BstV2I GAAGAC 1 cut(s) 965
BstZI CGGCCG 1 cut(s) 607
Bsu15I ATCGAT 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 8 cut(s) 110, 573, 598, 609, 630, 844, 857, 943
BsuTUI ATCGAT 1 cut(s) 1002
BtrI CACGTC 1 cut(s) 701
BtsCI GGATG 4 cut(s) 33, 88, 111, 963
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 1 cut(s) 810
Cac8I GCNNGC 8 cut(s) 104, 108, 418, 585, 690, 711, 887, 977
CfoI GCGC 1 cut(s) 891
Cfr10I RCCGGY 4 cut(s) 106, 630, 885, 939
Cfr13I GGNCC 2 cut(s) 562, 942
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 2 cut(s) 471, 730
Csp6I GTAC 4 cut(s) 436, 510, 541, 937
CviAII CATG 7 cut(s) 76, 421, 495, 588, 913, 972, 989
CviQI GTAC 4 cut(s) 436, 510, 541, 937
DdeI CTNAG 4 cut(s) 64, 322, 684, 789
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
DraIII CACNNNGTG 1 cut(s) 626
EaeI YGGCCR 5 cut(s) 108, 571, 596, 607, 628
EagI CGGCCG 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 607
Eco105I TACGTA 1 cut(s) 435
Eco130I CCWWGG 2 cut(s) 874, 945
Eco147I AGGCCT 1 cut(s) 857
Eco31I GGTCTC 1 cut(s) 839
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 562
Eco52I CGGCCG 1 cut(s) 607
Eco72I CACGTG 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 237, 598
EcoRV GATATC 1 cut(s) 88
EcoT14I CCWWGG 2 cut(s) 874, 945
ErhI CCWWGG 2 cut(s) 874, 945
FaeI CATG 7 cut(s) 79, 424, 498, 591, 916, 975, 992
FaqI GGGAC 3 cut(s) 295, 605, 786
FatI CATG 7 cut(s) 75, 420, 494, 587, 912, 971, 988
FblI GTMKAC 1 cut(s) 485
Fnu4HI GCNGC 9 cut(s) 61, 94, 190, 289, 427, 607, 618, 739, 815
FokI GGATG 4 cut(s) 20, 95, 98, 950
Fsp4HI GCNGC 9 cut(s) 61, 94, 190, 289, 427, 607, 618, 739, 815
FspBI CTAG 1 cut(s) 654
GlaI GCGC 1 cut(s) 890
GluI GCNGC 9 cut(s) 61, 94, 190, 289, 427, 607, 618, 739, 815
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 8 cut(s) 110, 573, 598, 609, 630, 844, 857, 943
HapII CCGG 8 cut(s) 107, 165, 222, 524, 580, 631, 886, 940
HgaI GACGC 2 cut(s) 471, 730
HhaI GCGC 1 cut(s) 891
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 7 cut(s) 79, 424, 498, 591, 916, 975, 992
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 2 cut(s) 149, 504
HpaI GTTAAC 1 cut(s) 765
HpaII CCGG 8 cut(s) 107, 165, 222, 524, 580, 631, 886, 940
HphI GGTGA 2 cut(s) 199, 478
Hpy166II GTNNAC 3 cut(s) 438, 486, 765
Hpy188I TCNGA 3 cut(s) 562, 934, 1044
Hpy188III TCNNGA 2 cut(s) 675, 836
Hpy8I GTNNAC 3 cut(s) 438, 486, 765
Hpy99I CGWCG 3 cut(s) 101, 562, 908
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 3 cut(s) 177, 442, 1056
HpyCH4IV ACGT 5 cut(s) 362, 434, 512, 622, 700
HpyCH4V TGCA 4 cut(s) 75, 420, 498, 999
HpyF10VI GCNNNNNNNGC 6 cut(s) 99, 426, 719, 747, 924, 981
HpyF3I CTNAG 4 cut(s) 64, 322, 684, 789
HpySE526I ACGT 5 cut(s) 362, 434, 512, 622, 700
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 7 cut(s) 79, 424, 498, 591, 916, 975, 992
HspAI GCGC 1 cut(s) 889
KroI GCCGGC 2 cut(s) 106, 885
KroNI GCCGGC 2 cut(s) 108, 887
KspAI GTTAAC 1 cut(s) 765
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 2 cut(s) 161, 814
Lsp1109I GCAGC 5 cut(s) 72, 201, 275, 725, 826
LweI GCATC 2 cut(s) 696, 1008
MaeI CTAG 1 cut(s) 654
MaeII ACGT 5 cut(s) 362, 434, 512, 622, 700
MaeIII GTNAC 1 cut(s) 182
MalI GATC 1 cut(s) 279
MbiI CCGCTC 1 cut(s) 426
MboI GATC 1 cut(s) 277
MboII GAAGA 8 cut(s) 19, 22, 31, 34, 37, 40, 450, 970
MhlI GDGCHC 2 cut(s) 158, 920
MlsI TGGCCA 2 cut(s) 573, 598
MluCI AATT 5 cut(s) 70, 116, 649, 796, 1045
MluNI TGGCCA 2 cut(s) 573, 598
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 5 cut(s) 60, 360, 540, 676, 1022
MnlI CCTC 9 cut(s) 47, 215, 302, 323, 456, 541, 693, 847, 860
Mox20I TGGCCA 2 cut(s) 573, 598
MroNI GCCGGC 2 cut(s) 106, 885
MroXI GAANNNNTTC 1 cut(s) 1011
MscI TGGCCA 2 cut(s) 573, 598
MseI TTAA 5 cut(s) 273, 369, 404, 764, 795
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 2 cut(s) 573, 598
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 8 cut(s) 107, 165, 222, 524, 580, 631, 886, 940
MspR9I CCNGG 4 cut(s) 165, 223, 239, 600
Mva1269I GAATGC 1 cut(s) 1027
MvaI CCWGG 2 cut(s) 239, 600
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 6 cut(s) 99, 426, 719, 747, 924, 981
NaeI GCCGGC 2 cut(s) 108, 887
NciI CCSGG 2 cut(s) 165, 223
NdeII GATC 1 cut(s) 277
NgoMIV GCCGGC 2 cut(s) 106, 885
NlaIII CATG 7 cut(s) 79, 424, 498, 591, 916, 975, 992
NlaIV GGNNCC 1 cut(s) 810
NmeAIII GCCGAG 1 cut(s) 870
NmuCI GTSAC 1 cut(s) 182
NspI RCATGY 3 cut(s) 79, 498, 916
PaeR7I CTCGAG 1 cut(s) 851
PceI AGGCCT 1 cut(s) 857
PciI ACATGT 1 cut(s) 912
PctI GAATGC 1 cut(s) 1027
PdiI GCCGGC 2 cut(s) 108, 887
PdmI GAANNNNTTC 1 cut(s) 1011
PfeI GAWTC 1 cut(s) 504
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 9 cut(s) 62, 95, 191, 290, 428, 608, 619, 740, 816
PleI GAGTC 1 cut(s) 157
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 1 cut(s) 157
Ppu21I YACGTR 2 cut(s) 435, 623
PscI ACATGT 1 cut(s) 912
Psp6I CCWGG 2 cut(s) 237, 598
PspCI CACGTG 1 cut(s) 623
PspGI CCWGG 2 cut(s) 237, 598
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 2 cut(s) 562, 942
PspXI VCTCGAGB 1 cut(s) 851
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 4 cut(s) 437, 511, 542, 938
RsaNI GTAC 4 cut(s) 436, 510, 541, 937
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 5 cut(s) 273, 369, 404, 764, 795
SatI GCNGC 9 cut(s) 61, 94, 190, 289, 427, 607, 618, 739, 815
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 2 cut(s) 562, 942
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 4 cut(s) 165, 223, 239, 600
SduI GDGCHC 2 cut(s) 158, 920
SfaNI GCATC 2 cut(s) 696, 1008
Sfr274I CTCGAG 1 cut(s) 851
SinI GGWCC 1 cut(s) 562
SlaI CTCGAG 1 cut(s) 851
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 2 cut(s) 673, 851
SmoI CTYRAG 2 cut(s) 673, 851
SnaBI TACGTA 1 cut(s) 435
Sse9I AATT 5 cut(s) 70, 116, 649, 796, 1045
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 7 cut(s) 93, 426, 545, 606, 618, 801, 812
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 654
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 4 cut(s) 163, 221, 237, 598
StyI CCWWGG 2 cut(s) 874, 945
TaaI ACNGT 3 cut(s) 177, 442, 1056
TaiI ACGT 5 cut(s) 365, 437, 515, 625, 703
TaqI TCGA 6 cut(s) 461, 491, 502, 837, 852, 1002
TasI AATT 5 cut(s) 70, 116, 649, 796, 1045
TauI GCSGC 4 cut(s) 96, 429, 609, 620
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 5 cut(s) 273, 369, 404, 764, 795
Tru9I TTAA 5 cut(s) 273, 369, 404, 764, 795
TscAI CASTG 1 cut(s) 810
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 5 cut(s) 60, 189, 288, 738, 814
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 17, 22, 1020, 1034
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 1 cut(s) 810
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 562
XapI RAATTY 1 cut(s) 1045
XceI RCATGY 3 cut(s) 79, 498, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.