MD07G1290600.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008674
Physical Location & Seq
Forward (+)
35126417 .. 35128877
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1290600.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGAATATTCCCAGAGCAATCTTGTCCTTCTTCATCCTCTTCTTCTTCTTGTCATCCTCATACTTTGCAAGCAGCTTAGAAAATTGCAAGGAGGCGGATATTTGCGGCGTCGGCGAGCCGGTCATCGAATTCCCGTTTGGTATACAAAAAAAGGGGCCAGAGTCATGCTCCTATCCCGGTTTTGAACTGTCCTGTGACAACACCAGCCAAACAATCCTCACCCTTCCTTATTCCGGACAGTTCACTCTCCACGATATAGACTATGCCGAACAAGAAATATGGATTAACGATCCAAACAAATGCCTCGCACAACGAATTTTGTCCCTCAATCTGTCGGGCTCTCCATTTTACGGTGTGACAAATGAAAGTTTCACTTACTTTAATTGCTCGTTGAATTATTTGAAATACACATTAAACCCTATTGCTTGCATGAGTGGCTCTACGTACACTGTATTTGCTACCACTTCTCCGGTGGTGATTAATTACTTGGCGTCCACATCAACATGCGATCCAATCGGTACGTTTGTTGTTCCGGTTGAATTCCCGTTGTATGAGGAGTTGATGTCGTCGGACCCGACTGGCAATCTCCGGCTCGCATGGGACAGGCCAGGGTGTGGCAGTTGCGCCGCACGTGGTGGCCGGTGTGGGTTTAAGAGTAATTCCAGTCATCAAGTTGTTTGCTCAAGACTTCCTCAACGAGGAATCCCAAGGAGCGCACGCTATGCTATTACTGTTGGAGTTGGAGTGCCAGGACTCATGTGCGTTTTGGGGGTTTTGTGTTGCGCCTTTGGCAAGGTCAAGTCTTTCACAAGGGGGCGCAGAACTCTGCCTGAATTTAATTCCGCAGCGGCTTCGCAGCCCACCATCGCCAGGGGCCTCGATAAACCAAGGCTCGAGGCCTACCCGAAAATAGTCCTCGGCGAAAGCCGGCGCTTACCAAAGCCCGACAACAGCACGTGCCCAATATGCTTGTCCGAGTACCAGCCCAAGGAGACATTGAAGACCATCCCCAAATGTCAACATTGCTTCCATGCAGATTGCATTGATGAATGGCTTCAAATGAATGCTACTTGCCCTATTTGTCGAAATTCTCCACCGTGA

Protein Analysis

367

Amino Acids

40.27

Weight (kDa)

7.72

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 97 8.9e-19 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 318 - 362 5e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 319 - 362 6.2e-14 Ring finger domain
zf-RING_11 PF17123 320 - 348 4.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 320 - 361 2.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 320 - 359 1.1e-06 RING-type zinc-finger
zf-C3HC4 PF00097 320 - 361 3.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 614
AccI GTMKAC 1 cut(s) 142
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 5 cut(s) 95, 105, 627, 843, 848
AclWI GGATC 2 cut(s) 284, 503
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 5 cut(s) 128, 315, 539, 833, 1087
AcvI CACGTG 2 cut(s) 632, 957
AcyI GRCGYC 2 cut(s) 108, 491
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 3 cut(s) 446, 520, 980
AfiI CCNNNNNNNGG 6 cut(s) 233, 350, 614, 698, 870, 988
AgsI TTSAA 6 cut(s) 185, 394, 403, 539, 1000, 1058
AjnI CCWGG 3 cut(s) 607, 748, 869
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 1 cut(s) 75
AluI AGCT 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 986
AlwI GGATC 2 cut(s) 284, 503
Ama87I CYCGRG 1 cut(s) 893
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 5 cut(s) 155, 605, 637, 874, 897
ApeKI GCWGC 3 cut(s) 72, 845, 856
ApoI RAATTY 5 cut(s) 128, 315, 539, 833, 1087
AseI ATTAAT 1 cut(s) 480
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 5 cut(s) 626, 716, 785, 819, 933
AspS9I GGNCC 3 cut(s) 155, 571, 874
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 2 cut(s) 211, 487
AvaI CYCGRG 1 cut(s) 893
AvaII GGWCC 1 cut(s) 571
BaeGI GKGCMC 1 cut(s) 962
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BanII GRGCYC 1 cut(s) 341
BbrPI CACGTG 2 cut(s) 632, 957
BbsI GAAGAC 1 cut(s) 1007
BbvI GCAGC 3 cut(s) 84, 857, 868
BccI CCATC 2 cut(s) 872, 1013
BcgI CGANNNNNNTGC 2 cut(s) 834, 868
BciT130I CCWGG 3 cut(s) 609, 750, 871
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 986
BfoI RGCGCY 1 cut(s) 934
BisI GCNGC 6 cut(s) 73, 106, 627, 846, 849, 857
BlsI GCNGC 6 cut(s) 74, 107, 628, 847, 850, 858
Bme1390I CCNGG 4 cut(s) 177, 609, 750, 871
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 893
BmgT120I GGNCC 3 cut(s) 155, 571, 874
BmiI GGNNCC 3 cut(s) 156, 573, 875
BmrFI CCNGG 4 cut(s) 177, 609, 750, 871
BpiI GAAGAC 1 cut(s) 1007
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpuEI CTTGAG 1 cut(s) 667
BpuMI CCSGG 1 cut(s) 177
BsaAI YACGTR 3 cut(s) 444, 632, 957
BsaHI GRCGYC 2 cut(s) 108, 491
BsaJI CCNNGG 6 cut(s) 608, 707, 870, 887, 916, 987
BsaWI WCCGGW 3 cut(s) 233, 469, 532
BsaXI ACNNNNNCTCC 6 cut(s) 451, 481, 548, 578, 729, 759
Bsc4I CCNNNNNNNGG 6 cut(s) 233, 350, 614, 698, 870, 988
Bse118I RCCGGY 3 cut(s) 118, 639, 927
Bse1I ACTGG 2 cut(s) 583, 663
Bse3DI GCAATG 1 cut(s) 1021
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 3 cut(s) 609, 750, 871
BseDI CCNNGG 6 cut(s) 608, 707, 870, 887, 916, 987
BseGI GGATG 3 cut(s) 33, 53, 1005
BseLI CCNNNNNNNGG 6 cut(s) 233, 350, 614, 698, 870, 988
BseMI GCAATG 1 cut(s) 1021
BseNI ACTGG 2 cut(s) 583, 663
BseRI GAGGAG 1 cut(s) 569
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 3 cut(s) 84, 857, 868
BshFI GGCC 5 cut(s) 157, 607, 639, 876, 899
BsiHKCI CYCGRG 1 cut(s) 893
BsiSI CCGG 8 cut(s) 119, 177, 234, 470, 533, 589, 640, 928
BslFI GGGAC 2 cut(s) 307, 614
BslI CCNNNNNNNGG 6 cut(s) 233, 350, 614, 698, 870, 988
BsmAI GTCTC 1 cut(s) 986
BsmFI GGGAC 2 cut(s) 307, 614
BsmI GAATGC 1 cut(s) 1069
BsnI GGCC 5 cut(s) 157, 607, 639, 876, 899
BsoBI CYCGRG 1 cut(s) 893
Bsp1286I GDGCHC 2 cut(s) 341, 962
Bsp13I TCCGGA 1 cut(s) 233
Bsp143I GATC 2 cut(s) 289, 508
BspACI CCGC 5 cut(s) 95, 105, 627, 843, 848
BspANI GGCC 5 cut(s) 157, 607, 639, 876, 899
BspEI TCCGGA 1 cut(s) 233
BspLI GGNNCC 3 cut(s) 156, 573, 875
BspPI GGATC 2 cut(s) 284, 503
BsrDI GCAATG 1 cut(s) 1021
BsrFI RCCGGY 3 cut(s) 118, 639, 927
BsrI ACTGG 2 cut(s) 583, 663
BssAI RCCGGY 3 cut(s) 118, 639, 927
BssECI CCNNGG 6 cut(s) 608, 707, 870, 887, 916, 987
BssMI GATC 2 cut(s) 289, 508
BssNAI GTATAC 1 cut(s) 143
BssNI GRCGYC 2 cut(s) 108, 491
BssT1I CCWWGG 3 cut(s) 707, 887, 987
Bst1107I GTATAC 1 cut(s) 143
Bst2UI CCWGG 3 cut(s) 609, 750, 871
Bst4CI ACNGT 6 cut(s) 189, 240, 353, 451, 733, 1098
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 2 cut(s) 108, 491
BstAPI GCANNNNNTGC 1 cut(s) 722
BstBAI YACGTR 3 cut(s) 444, 632, 957
BstC8I GCNNGC 6 cut(s) 70, 116, 427, 594, 718, 929
BstDEI CTNAG 1 cut(s) 76
BstENI CCTNNNNNAGG 1 cut(s) 696
BstF5I GGATG 3 cut(s) 33, 53, 1005
BstH2I RGCGCY 1 cut(s) 934
BstHHI GCGC 5 cut(s) 626, 716, 785, 819, 933
BstKTI GATC 2 cut(s) 292, 511
BstMAI GTCTC 1 cut(s) 986
BstMBI GATC 2 cut(s) 289, 508
BstMWI GCNNNNNNNGC 5 cut(s) 111, 435, 722, 789, 966
BstNI CCWGG 3 cut(s) 609, 750, 871
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 4 cut(s) 175, 607, 748, 869
BstSLI GKGCMC 1 cut(s) 962
BstSNI TACGTA 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 84, 857, 868
BstV2I GAAGAC 1 cut(s) 1007
BstZ17I GTATAC 1 cut(s) 143
BsuRI GGCC 5 cut(s) 157, 607, 639, 876, 899
BtgZI GCGATG 1 cut(s) 850
BtsCI GGATG 3 cut(s) 33, 53, 1005
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 6 cut(s) 70, 116, 427, 594, 718, 929
CfoI GCGC 5 cut(s) 626, 716, 785, 819, 933
Cfr10I RCCGGY 3 cut(s) 118, 639, 927
Cfr13I GGNCC 3 cut(s) 155, 571, 874
CseI GACGC 2 cut(s) 97, 480
Csp6I GTAC 3 cut(s) 445, 519, 979
CviAII CATG 6 cut(s) 165, 430, 504, 597, 757, 1031
CviQI GTAC 3 cut(s) 445, 519, 979
DdeI CTNAG 1 cut(s) 76
DpnI GATC 2 cut(s) 291, 510
DpnII GATC 2 cut(s) 289, 508
DraIII CACNNNGTG 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 110
Eco105I TACGTA 1 cut(s) 444
Eco130I CCWWGG 3 cut(s) 707, 887, 987
Eco147I AGGCCT 1 cut(s) 899
Eco24I GRGCYC 1 cut(s) 341
Eco47I GGWCC 1 cut(s) 571
Eco72I CACGTG 2 cut(s) 632, 957
Eco88I CYCGRG 1 cut(s) 893
EcoNI CCTNNNNNAGG 1 cut(s) 696
EcoO109I RGGNCCY 1 cut(s) 874
EcoRI GAATTC 2 cut(s) 128, 539
EcoRII CCWGG 3 cut(s) 607, 748, 869
EcoT14I CCWWGG 3 cut(s) 707, 887, 987
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 3 cut(s) 707, 887, 987
FaeI CATG 6 cut(s) 168, 433, 507, 600, 760, 1034
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 2 cut(s) 307, 614
FatI CATG 6 cut(s) 164, 429, 503, 596, 756, 1030
FblI GTMKAC 1 cut(s) 142
Fnu4HI GCNGC 6 cut(s) 73, 106, 627, 846, 849, 857
FokI GGATG 3 cut(s) 20, 40, 992
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 6 cut(s) 73, 106, 627, 846, 849, 857
GlaI GCGC 5 cut(s) 625, 715, 784, 818, 932
GluI GCNGC 6 cut(s) 73, 106, 627, 846, 849, 857
HaeII RGCGCY 1 cut(s) 934
HaeIII GGCC 5 cut(s) 157, 607, 639, 876, 899
HapII CCGG 8 cut(s) 119, 177, 234, 470, 533, 589, 640, 928
HgaI GACGC 2 cut(s) 97, 480
HhaI GCGC 5 cut(s) 626, 716, 785, 819, 933
Hin1I GRCGYC 2 cut(s) 108, 491
Hin1II CATG 6 cut(s) 168, 433, 507, 600, 760, 1034
Hin6I GCGC 5 cut(s) 624, 714, 783, 817, 931
HinP1I GCGC 5 cut(s) 624, 714, 783, 817, 931
HincII GTYRAC 1 cut(s) 1019
HindII GTYRAC 1 cut(s) 1019
HinfI GANTC 3 cut(s) 161, 702, 753
HpaII CCGG 8 cut(s) 119, 177, 234, 470, 533, 589, 640, 928
HphI GGTGA 2 cut(s) 211, 487
Hpy166II GTNNAC 5 cut(s) 143, 243, 447, 495, 1019
Hpy188I TCNGA 2 cut(s) 571, 976
Hpy188III TCNNGA 2 cut(s) 234, 684
Hpy8I GTNNAC 5 cut(s) 143, 243, 447, 495, 1019
Hpy99I CGWCG 2 cut(s) 113, 571
HpyAV CCTTC 2 cut(s) 37, 233
HpyCH4III ACNGT 6 cut(s) 189, 240, 353, 451, 733, 1098
HpyCH4IV ACGT 4 cut(s) 443, 521, 631, 956
HpyCH4V TGCA 5 cut(s) 68, 87, 429, 1034, 1041
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 435, 722, 789, 966
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 4 cut(s) 443, 521, 631, 956
Hsp92I GRCGYC 2 cut(s) 108, 491
Hsp92II CATG 6 cut(s) 168, 433, 507, 600, 760, 1034
HspAI GCGC 5 cut(s) 624, 714, 783, 817, 931
Kpn2I TCCGGA 1 cut(s) 233
KroI GCCGGC 1 cut(s) 927
KroNI GCCGGC 1 cut(s) 929
Kzo9I GATC 2 cut(s) 289, 508
LmnI GCTCC 2 cut(s) 173, 711
Lsp1109I GCAGC 3 cut(s) 84, 857, 868
MaeII ACGT 4 cut(s) 443, 521, 631, 956
MaeIII GTNAC 2 cut(s) 194, 355
MalI GATC 2 cut(s) 291, 510
MboI GATC 2 cut(s) 289, 508
MboII GAAGA 5 cut(s) 22, 31, 34, 37, 1012
MhlI GDGCHC 2 cut(s) 341, 962
MlyI GAGTC 2 cut(s) 170, 747
MmeI TCCRAC 3 cut(s) 549, 715, 721
MroI TCCGGA 1 cut(s) 233
MroNI GCCGGC 1 cut(s) 927
MroXI GAANNNNTTC 1 cut(s) 1053
MseI TTAA 6 cut(s) 285, 381, 413, 480, 651, 837
MslI CAYNNNNRTG 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 848
MspI CCGG 8 cut(s) 119, 177, 234, 470, 533, 589, 640, 928
MspR9I CCNGG 4 cut(s) 177, 609, 750, 871
Mva1269I GAATGC 1 cut(s) 1069
MvaI CCWGG 3 cut(s) 609, 750, 871
MwoI GCNNNNNNNGC 5 cut(s) 111, 435, 722, 789, 966
NaeI GCCGGC 1 cut(s) 929
NciI CCSGG 1 cut(s) 177
NdeII GATC 2 cut(s) 289, 508
NgoMIV GCCGGC 1 cut(s) 927
NlaIII CATG 6 cut(s) 168, 433, 507, 600, 760, 1034
NlaIV GGNNCC 3 cut(s) 156, 573, 875
NmeAIII GCCGAG 1 cut(s) 897
NmuCI GTSAC 2 cut(s) 194, 355
NspI RCATGY 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 893
PceI AGGCCT 1 cut(s) 899
PcsI WCGNNNNNNNCGW 1 cut(s) 572
PctI GAATGC 1 cut(s) 1069
PdiI GCCGGC 1 cut(s) 929
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 1 cut(s) 702
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 6 cut(s) 74, 107, 628, 847, 850, 858
PleI GAGTC 2 cut(s) 169, 747
PmaCI CACGTG 2 cut(s) 632, 957
PmlI CACGTG 2 cut(s) 632, 957
PpsI GAGTC 2 cut(s) 169, 747
Ppu21I YACGTR 3 cut(s) 444, 632, 957
PshBI ATTAAT 1 cut(s) 480
Psp6I CCWGG 3 cut(s) 607, 748, 869
PspCI CACGTG 2 cut(s) 632, 957
PspGI CCWGG 3 cut(s) 607, 748, 869
PspN4I GGNNCC 3 cut(s) 156, 573, 875
PspPI GGNCC 3 cut(s) 155, 571, 874
PspXI VCTCGAGB 1 cut(s) 893
RsaI GTAC 3 cut(s) 446, 520, 980
RsaNI GTAC 3 cut(s) 445, 519, 979
RseI CAYNNNNRTG 1 cut(s) 502
SaqAI TTAA 6 cut(s) 285, 381, 413, 480, 651, 837
SatI GCNGC 6 cut(s) 73, 106, 627, 846, 849, 857
Sau3AI GATC 2 cut(s) 289, 508
Sau96I GGNCC 3 cut(s) 155, 571, 874
SchI GAGTC 2 cut(s) 170, 747
ScrFI CCNGG 4 cut(s) 177, 609, 750, 871
SduI GDGCHC 2 cut(s) 341, 962
SetI ASST 6 cut(s) 77, 446, 524, 634, 798, 959
Sfr274I CTCGAG 1 cut(s) 893
SinI GGWCC 1 cut(s) 571
SlaI CTCGAG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 2 cut(s) 682, 893
SmoI CTYRAG 2 cut(s) 682, 893
SnaBI TACGTA 1 cut(s) 444
SseBI AGGCCT 1 cut(s) 899
SsiI CCGC 5 cut(s) 95, 105, 627, 843, 848
SspI AATATT 1 cut(s) 7
StuI AGGCCT 1 cut(s) 899
StyD4I CCNGG 4 cut(s) 175, 607, 748, 869
StyI CCWWGG 3 cut(s) 707, 887, 987
TaaI ACNGT 6 cut(s) 189, 240, 353, 451, 733, 1098
TaiI ACGT 4 cut(s) 446, 524, 634, 959
TaqI TCGA 4 cut(s) 126, 879, 894, 1084
TauI GCSGC 3 cut(s) 108, 629, 851
TfiI GAWTC 1 cut(s) 702
Tru1I TTAA 6 cut(s) 285, 381, 413, 480, 651, 837
Tru9I TTAA 6 cut(s) 285, 381, 413, 480, 651, 837
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 2 cut(s) 194, 355
TseI GCWGC 3 cut(s) 72, 845, 856
Tsp45I GTSAC 2 cut(s) 194, 355
TspDTI ATGAA 5 cut(s) 17, 22, 378, 1062, 1076
TspRI CASTG 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 571
VspI ATTAAT 1 cut(s) 480
XagI CCTNNNNNAGG 1 cut(s) 696
XapI RAATTY 5 cut(s) 128, 315, 539, 833, 1087
XceI RCATGY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XhoI CTCGAG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.