RLG00000026251

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008674
Physical Location & Seq
Reverse (-)
1622566 .. 1625017
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026251

Sequence Viewer

Length: 1086 bp
ATGAATACTGTCAGAGCCATCATCTTCTCTTTCCTTCTCTTCTCTTCATTCTCATGCCTTACAACGAGTAGTACCTTAGAGACTTGCAAAAAAGCTGTTTGCCAAACAGGCGAGGCCGTAATCCGGTTCCCGTTTCGGATCGACAAACGCCAGCCGGAGACTTGCGGCTTTCCCGGTTTTGATCTGACCTGTGACATCACATCCCAAACACTGCTCAATCTTCCTTCTTCGGGAGATTTCACCGTTCAGGGCATAGACTACGGCAAGCAAGAGATATGGATCAACGATCCAAACACCTGCCTCCCCAAACGGCTCCTCTCTCTGAACCTCACCGGCTCTCCGTTTCGGGCTGGGTTTAGTGAAGACTTCACGTTCTTTAACTGCTCCTTGGACTACTTGAAGTACAAGCTAAACCCTATTGCTTGCATGAGTGGTTCTAACTACGCGGTTTTCGCCACCAATTCCGCGAGGGTGATTAGGTACTTGTCGACGTGCAGTAAGATCGGAACGTTTTCTGTTCCGGTCGAGTGGACGTTCCACCAGGAGGTTATGTCGTCCGACCTCGACGTTCATCTCCGGCTAAAGTGGGACCGACCGGGGTGCGGCACCTGCGAGTTGCGTGGCGGAACGTGTGGCTTCAAGACCAATTCTACTAAAGTTGTTTGCTCCAGCCTTAATCAACGAGGAATCCCCAGGGGTGCACGCTATGCTATTACAGTGGGCGTTGGAGTGCCAGCAATTATGTGCCTTATAGGGCTATTATGTTGCATGTGTGGCAGACTCAAGCCTTTAACAATGAGACGCACATCACTCCCAGAATTTAATTCAATTGGGGCTCCGCAGCCTGGAATCGTAAAGGGACTCGATGTGGCAACACTAGCGGCTTACCCGAAAATAGTCCTCGGTGAAAGCCGGCGTCTACCGCAGCCGGATGATAGCACTTGTTCGATATGCTTGTCCGAATACCGGCCGAAGGAGACACTAAAGACCATACCCGAATGCCAACATTGCTTCCATGCTAATTGCATCGATGAATGGCTTAAATTGAATGCTACTTGCCCTATTTGTAGAAAATCTCCACAATGA

Protein Analysis

362

Amino Acids

39.9

Weight (kDa)

8.68

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 96 4.5e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 155 - 223 5.5e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 314 - 357 9e-13 Ring finger domain
zf-rbx1 PF12678 314 - 357 8e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 315 - 343 1.4e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 315 - 356 1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 305, 617
Acc36I ACCTGC 2 cut(s) 305, 617
AccB1I GGYRCC 1 cut(s) 605
AccI GTMKAC 2 cut(s) 488, 919
AccII CGCG 2 cut(s) 446, 467
AciI CCGC 8 cut(s) 165, 446, 465, 603, 624, 839, 881, 923
AclI AACGTT 1 cut(s) 509
AclWI GGATC 3 cut(s) 146, 281, 287
AcoI YGGCCR 1 cut(s) 968
AcsI RAATTY 1 cut(s) 818
AcyI GRCGYC 1 cut(s) 916
AfaI GTAC 3 cut(s) 73, 404, 482
AfiI CCNNNNNNNGG 7 cut(s) 123, 230, 544, 602, 845, 966, 973
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 4 cut(s) 400, 640, 828, 1048
AjiI CACGTC 1 cut(s) 492
AjnI CCWGG 3 cut(s) 540, 692, 844
AluBI AGCT 2 cut(s) 95, 409
AluI AGCT 2 cut(s) 95, 409
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 4 cut(s) 74, 152, 793, 971
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 3 cut(s) 146, 281, 287
AoxI GGCC 2 cut(s) 114, 968
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 2 cut(s) 841, 925
ApoI RAATTY 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 589
AsuC2I CCSGG 2 cut(s) 174, 597
AsuHPI GGTGA 4 cut(s) 232, 322, 484, 917
AvaII GGWCC 1 cut(s) 589
BaeGI GKGCMC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 838
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 703
BbvI GCAGC 2 cut(s) 853, 937
BccI CCATC 1 cut(s) 26
BceAI ACGGC 3 cut(s) 101, 277, 326
BcgI CGANNNNNNTGC 4 cut(s) 484, 518, 582, 616
BciT130I CCWGG 3 cut(s) 542, 694, 846
BcnI CCSGG 2 cut(s) 174, 597
BcoDI GTCTC 4 cut(s) 74, 152, 793, 971
BfaI CTAG 1 cut(s) 878
BfuAI ACCTGC 2 cut(s) 305, 617
BglI GCCNNNNNGGC 1 cut(s) 108
BisI GCNGC 5 cut(s) 166, 604, 842, 882, 926
BlsI GCNGC 5 cut(s) 167, 605, 843, 883, 927
Bme1390I CCNGG 5 cut(s) 174, 542, 597, 694, 846
Bme18I GGWCC 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 5 cut(s) 128, 314, 590, 607, 837
BmrFI CCNGG 5 cut(s) 174, 542, 597, 694, 846
BmsI GCATC 1 cut(s) 1035
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 652
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 174, 597
Bsa29I ATCGAT 1 cut(s) 1029
BsaBI GATNNNNATC 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 916
BsaJI CCNNGG 5 cut(s) 387, 596, 692, 693, 901
BsaWI WCCGGW 2 cut(s) 123, 520
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 536, 566
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 230, 544, 602, 845, 966, 973
Bse118I RCCGGY 3 cut(s) 332, 912, 966
Bse3DI GCAATG 1 cut(s) 1006
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 3 cut(s) 542, 694, 846
BseCI ATCGAT 1 cut(s) 1029
BseDI CCNNGG 5 cut(s) 387, 596, 692, 693, 901
BseGI GGATG 2 cut(s) 200, 937
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 7 cut(s) 123, 230, 544, 602, 845, 966, 973
BseMI GCAATG 1 cut(s) 1006
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 703
BseX3I CGGCCG 1 cut(s) 968
BseXI GCAGC 2 cut(s) 853, 937
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 2 cut(s) 446, 467
Bsh1285I CGRYCG 3 cut(s) 525, 596, 971
BshFI GGCC 2 cut(s) 116, 970
BshNI GGYRCC 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 1029
BsiEI CGRYCG 3 cut(s) 525, 596, 971
BsiHKAI GWGCWC 1 cut(s) 703
BslFI GGGAC 2 cut(s) 602, 873
BslI CCNNNNNNNGG 7 cut(s) 123, 230, 544, 602, 845, 966, 973
BsmAI GTCTC 4 cut(s) 74, 152, 793, 971
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 2 cut(s) 602, 873
BsmI GAATGC 2 cut(s) 1004, 1054
BsnI GGCC 2 cut(s) 116, 970
Bsp1286I GDGCHC 2 cut(s) 703, 838
Bsp143I GATC 5 cut(s) 138, 181, 279, 286, 501
BspACI CCGC 8 cut(s) 165, 446, 465, 603, 624, 839, 881, 923
BspANI GGCC 2 cut(s) 116, 970
BspDI ATCGAT 1 cut(s) 1029
BspFNI CGCG 2 cut(s) 446, 467
BspLI GGNNCC 5 cut(s) 128, 314, 590, 607, 837
BspMI ACCTGC 2 cut(s) 305, 617
BspPI GGATC 3 cut(s) 146, 281, 287
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 3 cut(s) 332, 912, 966
BssAI RCCGGY 3 cut(s) 332, 912, 966
BssECI CCNNGG 5 cut(s) 387, 596, 692, 693, 901
BssMI GATC 5 cut(s) 138, 181, 279, 286, 501
BssNI GRCGYC 1 cut(s) 916
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 542, 694, 846
Bst4CI ACNGT 3 cut(s) 10, 244, 718
Bst6I CTCTTC 2 cut(s) 44, 49
BstACI GRCGYC 1 cut(s) 916
BstAPI GCANNNNNTGC 1 cut(s) 707
BstC8I GCNNGC 6 cut(s) 152, 266, 424, 703, 735, 914
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 200, 937
BstFNI CGCG 2 cut(s) 446, 467
BstKTI GATC 5 cut(s) 141, 184, 282, 289, 504
BstMAI GTCTC 4 cut(s) 74, 152, 793, 971
BstMBI GATC 5 cut(s) 138, 181, 279, 286, 501
BstMCI CGRYCG 3 cut(s) 525, 596, 971
BstMWI GCNNNNNNNGC 8 cut(s) 108, 452, 609, 707, 774, 878, 922, 1008
BstNI CCWGG 3 cut(s) 542, 694, 846
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 5 cut(s) 172, 540, 595, 692, 844
BstSLI GKGCMC 1 cut(s) 703
BstUI CGCG 2 cut(s) 446, 467
BstV1I GCAGC 2 cut(s) 853, 937
BstV2I GAAGAC 1 cut(s) 369
BstZI CGGCCG 1 cut(s) 968
Bsu15I ATCGAT 1 cut(s) 1029
BsuRI GGCC 2 cut(s) 116, 970
BsuTUI ATCGAT 1 cut(s) 1029
BtrI CACGTC 1 cut(s) 492
BtsCI GGATG 2 cut(s) 200, 937
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 2 cut(s) 209, 723
BveI ACCTGC 2 cut(s) 305, 617
Cac8I GCNNGC 6 cut(s) 152, 266, 424, 703, 735, 914
Cfr10I RCCGGY 3 cut(s) 332, 912, 966
Cfr13I GGNCC 1 cut(s) 589
ClaI ATCGAT 1 cut(s) 1029
CseI GACGC 2 cut(s) 810, 905
Csp6I GTAC 3 cut(s) 72, 403, 481
CviAII CATG 4 cut(s) 54, 427, 769, 1016
CviQI GTAC 3 cut(s) 72, 403, 481
DdeI CTNAG 1 cut(s) 76
DpnI GATC 5 cut(s) 140, 183, 281, 288, 503
DpnII GATC 5 cut(s) 138, 181, 279, 286, 501
EaeI YGGCCR 1 cut(s) 968
EagI CGGCCG 1 cut(s) 968
Eam1104I CTCTTC 2 cut(s) 44, 49
EarI CTCTTC 2 cut(s) 44, 49
EciI GGCGGA 1 cut(s) 639
EclXI CGGCCG 1 cut(s) 968
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 589
Eco52I CGGCCG 1 cut(s) 968
EcoRII CCWGG 3 cut(s) 540, 692, 844
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 838
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 1 cut(s) 793
FaeI CATG 4 cut(s) 57, 430, 772, 1019
FaqI GGGAC 2 cut(s) 602, 873
FatI CATG 4 cut(s) 53, 426, 768, 1015
FblI GTMKAC 2 cut(s) 488, 919
Fnu4HI GCNGC 5 cut(s) 166, 604, 842, 882, 926
FokI GGATG 2 cut(s) 187, 944
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 5 cut(s) 166, 604, 842, 882, 926
FspBI CTAG 1 cut(s) 878
GluI GCNGC 5 cut(s) 166, 604, 842, 882, 926
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 652
HaeIII GGCC 2 cut(s) 116, 970
HgaI GACGC 2 cut(s) 810, 905
Hin1I GRCGYC 1 cut(s) 916
Hin1II CATG 4 cut(s) 57, 430, 772, 1019
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 4 cut(s) 687, 780, 849, 861
HphI GGTGA 4 cut(s) 232, 322, 484, 917
Hpy166II GTNNAC 4 cut(s) 489, 531, 701, 920
Hpy188I TCNGA 7 cut(s) 14, 138, 186, 324, 506, 559, 961
Hpy188III TCNNGA 2 cut(s) 231, 640
Hpy8I GTNNAC 4 cut(s) 489, 531, 701, 920
Hpy99I CGWCG 2 cut(s) 493, 569
HpyAV CCTTC 3 cut(s) 44, 234, 967
HpyCH4III ACNGT 3 cut(s) 10, 244, 718
HpyCH4IV ACGT 6 cut(s) 371, 491, 509, 533, 567, 629
HpyCH4V TGCA 6 cut(s) 87, 426, 495, 701, 768, 1026
HpyF10VI GCNNNNNNNGC 8 cut(s) 108, 452, 609, 707, 774, 878, 922, 1008
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 6 cut(s) 371, 491, 509, 533, 567, 629
Hsp92I GRCGYC 1 cut(s) 916
Hsp92II CATG 4 cut(s) 57, 430, 772, 1019
KroI GCCGGC 1 cut(s) 912
KroNI GCCGGC 1 cut(s) 914
Kzo9I GATC 5 cut(s) 138, 181, 279, 286, 501
LmnI GCTCC 4 cut(s) 318, 389, 671, 841
Lsp1109I GCAGC 2 cut(s) 853, 937
LweI GCATC 1 cut(s) 1035
MaeI CTAG 1 cut(s) 878
MaeII ACGT 6 cut(s) 371, 491, 509, 533, 567, 629
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 140, 183, 281, 288, 503
MboI GATC 5 cut(s) 138, 181, 279, 286, 501
MboII GAAGA 6 cut(s) 16, 31, 36, 212, 219, 374
MfeI CAATTG 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 703, 838
MluCI AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
MlyI GAGTC 2 cut(s) 774, 855
MmeI TCCRAC 2 cut(s) 582, 706
MnlI CCTC 9 cut(s) 106, 311, 326, 338, 462, 538, 572, 677, 911
MroNI GCCGGC 1 cut(s) 912
MroXI GAANNNNTTC 1 cut(s) 511
MseI TTAA 5 cut(s) 378, 675, 791, 822, 1041
MslI CAYNNNNRTG 1 cut(s) 52
MspR9I CCNGG 5 cut(s) 174, 542, 597, 694, 846
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 2 cut(s) 1004, 1054
MvaI CCWGG 3 cut(s) 542, 694, 846
MvnI CGCG 2 cut(s) 446, 467
MwoI GCNNNNNNNGC 8 cut(s) 108, 452, 609, 707, 774, 878, 922, 1008
NaeI GCCGGC 1 cut(s) 914
NciI CCSGG 2 cut(s) 174, 597
NdeII GATC 5 cut(s) 138, 181, 279, 286, 501
NgoMIV GCCGGC 1 cut(s) 912
NlaIII CATG 4 cut(s) 57, 430, 772, 1019
NlaIV GGNNCC 5 cut(s) 128, 314, 590, 607, 837
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 772
PaqCI CACCTGC 2 cut(s) 305, 617
PasI CCCWGGG 1 cut(s) 693
PctI GAATGC 2 cut(s) 1004, 1054
PdiI GCCGGC 1 cut(s) 914
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 687, 849
PkrI GCNGC 5 cut(s) 167, 605, 843, 883, 927
PleI GAGTC 2 cut(s) 774, 855
PpsI GAGTC 2 cut(s) 774, 855
Psp1406I AACGTT 1 cut(s) 509
Psp6I CCWGG 3 cut(s) 540, 692, 844
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 3 cut(s) 540, 692, 844
PspN4I GGNNCC 5 cut(s) 128, 314, 590, 607, 837
PspPI GGNCC 1 cut(s) 589
RsaI GTAC 3 cut(s) 73, 404, 482
RsaNI GTAC 3 cut(s) 72, 403, 481
RseI CAYNNNNRTG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 487
SaqAI TTAA 5 cut(s) 378, 675, 791, 822, 1041
SatI GCNGC 5 cut(s) 166, 604, 842, 882, 926
Sau3AI GATC 5 cut(s) 138, 181, 279, 286, 501
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 2 cut(s) 774, 855
ScrFI CCNGG 5 cut(s) 174, 542, 597, 694, 846
SduI GDGCHC 2 cut(s) 703, 838
SfaNI GCATC 1 cut(s) 1035
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
SsiI CCGC 8 cut(s) 165, 446, 465, 603, 624, 839, 881, 923
SspMI CTAG 1 cut(s) 878
StyD4I CCNGG 5 cut(s) 172, 540, 595, 692, 844
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 3 cut(s) 10, 244, 718
TaiI ACGT 6 cut(s) 374, 494, 512, 536, 570, 632
TaqI TCGA 7 cut(s) 141, 488, 525, 564, 864, 947, 1029
TaqII GACCGA 1 cut(s) 606
TasI AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
TatI WGTACW 1 cut(s) 402
TauI GCSGC 3 cut(s) 168, 606, 884
TfiI GAWTC 2 cut(s) 687, 849
Tru1I TTAA 5 cut(s) 378, 675, 791, 822, 1041
Tru9I TTAA 5 cut(s) 378, 675, 791, 822, 1041
TscAI CASTG 2 cut(s) 216, 723
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 2 cut(s) 841, 925
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 4 cut(s) 17, 36, 560, 1047
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 2 cut(s) 216, 723
VneI GTGCAC 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 1 cut(s) 818
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 488, 919
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.