Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008674

Physical Location & Seq

Genomic Coordinates Forward (+)

35130444 .. 35132390

Gene Structure 1947 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1290700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 489 bp

ATGTCGTCGGACCTGACTAGCCATCTCGGGCTTGCATGGGACAGGCCAGGGTGCGGCAGTTGCTCCACAAGTGGTGGCCGGTGTGGGAATCCCAAGGAGCGTACGCTATGCTATTATTGTTGGAGTTGGAGTGCCAGGAAACTTGTGCGTTTTGGGGGGTTTTGTGTTGCGTCTTTGTCAAGGTCAAGTTTTTCACAAGGGGGCGCAGAACTCTCCCTGAGTTTAATTCCACAGTGGCTCCGCAGCCCAACATCGTCAGAGGCCTCGATAAGCCAAGGCTTGAGGGCTTACCTAAAAATAGTCCTCGGCGAAAGCCGCCGCTTACCAAAGCCCGACGACAGCATGTGCCCGATATGCTTGTCCGCGTACCGGCCCAAGGAGACATTGAAGACCATCCTCAAATGCCAACATTGCTTCCATGCAGATTGCGTGGATGTATGGCTTCAAATGAATGCTACTTGCCCTATTTGTCGGAATTCTCCACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

163

Amino Acids

17.83

Weight (kDa)

8.95

Isoelectric Point (pI)

52.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	115 - 158	2.1e-13	Ring finger domain
zf-C3HC4_2	PF13923	115 - 157	2.9e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	115 - 158	5.8e-07	RING-H2 zinc finger domain
zf-RING_11	PF17123	116 - 144	1.7e-07	RING-like zinc finger
zf-RING_UBOX	PF13445	116 - 155	1.5e-06	RING-type zinc-finger
zf-C3HC4	PF00097	116 - 157	1.8e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0008674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53110
fragaria_vesca	FvH4_7g31800 FvH4_7g31800
malus_domestica	MD01G1219300.v1.1 MD07G1290600.v1.1 MD07G1290700.v1.1
prunus_persica	Prupe.2G312000_v2.0.a1
pyrus_communis	pycom01g22910 pycom07g26340
rosa_chinensis	RchiOBHm_Chr1g0381031
rosa_laevigata	RLG00000026251
rosa_multiflora	Rmu_sc0004494.1_g000003 Rmu_sc0012952.1_g000007 Rmu_sc0028225.1_g000001 Rmu_sc0035898.1_g000001 Rmu_sc0039326.1_g000002
rosa_roxburghii	Rroxscaffold_4G00278840
rosa_rugosa	Rorug01G0426600
rosa_samantha	Rh1AG450400 Rh1BG406400 Rh1CG420400 Rh1DG436900
rosa_wichuraiana	Rw1G039250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	365
AciI	CCGC	5 cut(s)	54, 241, 316, 319, 363
AcoI	YGGCCR	1 cut(s)	76
AcsI	RAATTY	1 cut(s)	475
AfaI	GTAC	2 cut(s)	103, 368
AfiI	CCNNNNNNNGG	3 cut(s)	53, 369, 376
AgsI	TTSAA	2 cut(s)	388, 446
AjnI	CCWGG	2 cut(s)	46, 134
Alw26I	GTCTC	1 cut(s)	374
Ama87I	CYCGRG	1 cut(s)	26
AoxI	GGCC	4 cut(s)	44, 76, 261, 371
ApeKI	GCWGC	1 cut(s)	243
ApoI	RAATTY	1 cut(s)	475
AspLEI	GCGC	1 cut(s)	206
AspS9I	GGNCC	2 cut(s)	10, 372
AvaI	CYCGRG	1 cut(s)	26
AvaII	GGWCC	1 cut(s)	10
BaeGI	GKGCMC	1 cut(s)	350
BbsI	GAAGAC	1 cut(s)	395
BbvI	GCAGC	1 cut(s)	255
BccI	CCATC	2 cut(s)	30, 401
BciT130I	CCWGG	2 cut(s)	48, 136
BcoDI	GTCTC	1 cut(s)	374
BfaI	CTAG	1 cut(s)	18
BisI	GCNGC	4 cut(s)	55, 244, 316, 319
BlsI	GCNGC	4 cut(s)	56, 245, 317, 320
Bme1390I	CCNGG	2 cut(s)	48, 136
Bme18I	GGWCC	1 cut(s)	10
BmeT110I	CYCGRG	1 cut(s)	26
BmgT120I	GGNCC	2 cut(s)	10, 372
BmiI	GGNNCC	1 cut(s)	239
BmrFI	CCNGG	2 cut(s)	48, 136
BpiI	GAAGAC	1 cut(s)	395
BpuEI	CTTGAG	1 cut(s)	301
BsaJI	CCNNGG	5 cut(s)	47, 93, 274, 304, 375
BsaXI	ACNNNNNCTCC	4 cut(s)	115, 145, 222, 252
Bsc4I	CCNNNNNNNGG	3 cut(s)	53, 369, 376
Bse118I	RCCGGY	2 cut(s)	78, 369
Bse3DI	GCAATG	1 cut(s)	409
BseBI	CCWGG	2 cut(s)	48, 136
BseDI	CCNNGG	5 cut(s)	47, 93, 274, 304, 375
BseGI	GGATG	2 cut(s)	393, 439
BseLI	CCNNNNNNNGG	3 cut(s)	53, 369, 376
BseMI	GCAATG	1 cut(s)	409
BseMII	CTCAG	1 cut(s)	209
BseSI	GKGCMC	1 cut(s)	350
BseXI	GCAGC	1 cut(s)	255
Bsh1236I	CGCG	1 cut(s)	365
BshFI	GGCC	4 cut(s)	46, 78, 263, 373
BsiHKCI	CYCGRG	1 cut(s)	26
BsiSI	CCGG	2 cut(s)	79, 370
BsiWI	CGTACG	1 cut(s)	101
BslFI	GGGAC	1 cut(s)	53
BslI	CCNNNNNNNGG	3 cut(s)	53, 369, 376
BsmAI	GTCTC	1 cut(s)	374
BsmFI	GGGAC	1 cut(s)	53
BsmI	GAATGC	1 cut(s)	457
BsnI	GGCC	4 cut(s)	46, 78, 263, 373
BsoBI	CYCGRG	1 cut(s)	26
Bsp1286I	GDGCHC	1 cut(s)	350
BspACI	CCGC	5 cut(s)	54, 241, 316, 319, 363
BspANI	GGCC	4 cut(s)	46, 78, 263, 373
BspCNI	CTCAG	1 cut(s)	210
BspFNI	CGCG	1 cut(s)	365
BspLI	GGNNCC	1 cut(s)	239
BsrDI	GCAATG	1 cut(s)	409
BsrFI	RCCGGY	2 cut(s)	78, 369
BssAI	RCCGGY	2 cut(s)	78, 369
BssECI	CCNNGG	5 cut(s)	47, 93, 274, 304, 375
BssT1I	CCWWGG	3 cut(s)	93, 274, 375
Bst2UI	CCWGG	2 cut(s)	48, 136
Bst4CI	ACNGT	2 cut(s)	234, 486
BstC8I	GCNNGC	1 cut(s)	33
BstDEI	CTNAG	1 cut(s)	218
BstF5I	GGATG	2 cut(s)	393, 439
BstFNI	CGCG	1 cut(s)	365
BstHHI	GCGC	1 cut(s)	206
BstMAI	GTCTC	1 cut(s)	374
BstMWI	GCNNNNNNNGC	4 cut(s)	60, 315, 354, 411
BstNI	CCWGG	2 cut(s)	48, 136
BstNSI	RCATGY	1 cut(s)	346
BstSCI	CCNGG	2 cut(s)	46, 134
BstSLI	GKGCMC	1 cut(s)	350
BstUI	CGCG	1 cut(s)	365
BstV1I	GCAGC	1 cut(s)	255
BstV2I	GAAGAC	1 cut(s)	395
BsuRI	GGCC	4 cut(s)	46, 78, 263, 373
BtsCI	GGATG	2 cut(s)	393, 439
BtsIMutI	CAGTG	1 cut(s)	239
Cac8I	GCNNGC	1 cut(s)	33
CfoI	GCGC	1 cut(s)	206
Cfr10I	RCCGGY	2 cut(s)	78, 369
Cfr13I	GGNCC	2 cut(s)	10, 372
CseI	GACGC	1 cut(s)	159
Csp6I	GTAC	2 cut(s)	102, 367
CviAII	CATG	3 cut(s)	36, 343, 419
CviQI	GTAC	2 cut(s)	102, 367
DdeI	CTNAG	1 cut(s)	218
EaeI	YGGCCR	1 cut(s)	76
Eco130I	CCWWGG	3 cut(s)	93, 274, 375
Eco147I	AGGCCT	1 cut(s)	263
Eco47I	GGWCC	1 cut(s)	10
Eco88I	CYCGRG	1 cut(s)	26
EcoRI	GAATTC	1 cut(s)	475
EcoRII	CCWGG	2 cut(s)	46, 134
EcoT14I	CCWWGG	3 cut(s)	93, 274, 375
ErhI	CCWWGG	3 cut(s)	93, 274, 375
FaeI	CATG	3 cut(s)	39, 346, 422
FaiI	YATR	6 cut(s)	37, 109, 344, 355, 420, 439
FaqI	GGGAC	1 cut(s)	53
FatI	CATG	3 cut(s)	35, 342, 418
Fnu4HI	GCNGC	4 cut(s)	55, 244, 316, 319
FokI	GGATG	2 cut(s)	380, 446
Fsp4HI	GCNGC	4 cut(s)	55, 244, 316, 319
FspBI	CTAG	1 cut(s)	18
GlaI	GCGC	1 cut(s)	205
GluI	GCNGC	4 cut(s)	55, 244, 316, 319
HaeIII	GGCC	4 cut(s)	46, 78, 263, 373
HapII	CCGG	2 cut(s)	79, 370
HgaI	GACGC	1 cut(s)	159
HhaI	GCGC	1 cut(s)	206
Hin1II	CATG	3 cut(s)	39, 346, 422
Hin6I	GCGC	1 cut(s)	204
HinP1I	GCGC	1 cut(s)	204
HinfI	GANTC	1 cut(s)	88
HpaII	CCGG	2 cut(s)	79, 370
Hpy188I	TCNGA	3 cut(s)	10, 259, 474
Hpy99I	CGWCG	2 cut(s)	10, 338
HpyCH4III	ACNGT	2 cut(s)	234, 486
HpyCH4V	TGCA	2 cut(s)	35, 422
HpyF10VI	GCNNNNNNNGC	4 cut(s)	60, 315, 354, 411
HpyF3I	CTNAG	1 cut(s)	218
Hsp92II	CATG	3 cut(s)	39, 346, 422
HspAI	GCGC	1 cut(s)	204
LmnI	GCTCC	3 cut(s)	68, 97, 243
LpnPI	CCDG	9 cut(s)	26, 28, 33, 60, 92, 121, 148, 230, 383
Lsp1109I	GCAGC	1 cut(s)	255
MaeI	CTAG	1 cut(s)	18
MboII	GAAGA	1 cut(s)	400
MhlI	GDGCHC	1 cut(s)	350
MluCI	AATT	2 cut(s)	225, 475
MmeI	TCCRAC	3 cut(s)	101, 107, 452
MnlI	CCTC	5 cut(s)	253, 274, 276, 314, 407
MseI	TTAA	1 cut(s)	224
MspI	CCGG	2 cut(s)	79, 370
MspR9I	CCNGG	2 cut(s)	48, 136
Mva1269I	GAATGC	1 cut(s)	457
MvaI	CCWGG	2 cut(s)	48, 136
MvnI	CGCG	1 cut(s)	365
MwoI	GCNNNNNNNGC	4 cut(s)	60, 315, 354, 411
NlaIII	CATG	3 cut(s)	39, 346, 422
NlaIV	GGNNCC	1 cut(s)	239
NmeAIII	GCCGAG	1 cut(s)	285
NspI	RCATGY	1 cut(s)	346
PceI	AGGCCT	1 cut(s)	263
PctI	GAATGC	1 cut(s)	457
PfeI	GAWTC	1 cut(s)	88
Pfl23II	CGTACG	1 cut(s)	101
PkrI	GCNGC	4 cut(s)	56, 245, 317, 320
Psp6I	CCWGG	2 cut(s)	46, 134
PspGI	CCWGG	2 cut(s)	46, 134
PspLI	CGTACG	1 cut(s)	101
PspN4I	GGNNCC	1 cut(s)	239
PspPI	GGNCC	2 cut(s)	10, 372
RsaI	GTAC	2 cut(s)	103, 368
RsaNI	GTAC	2 cut(s)	102, 367
SaqAI	TTAA	1 cut(s)	224
SatI	GCNGC	4 cut(s)	55, 244, 316, 319
Sau96I	GGNCC	2 cut(s)	10, 372
ScrFI	CCNGG	2 cut(s)	48, 136
SduI	GDGCHC	1 cut(s)	350
SetI	ASST	3 cut(s)	15, 185, 294
SinI	GGWCC	1 cut(s)	10
SmlI	CTYRAG	1 cut(s)	280
SmoI	CTYRAG	1 cut(s)	280
Sse9I	AATT	2 cut(s)	225, 475
SseBI	AGGCCT	1 cut(s)	263
SsiI	CCGC	5 cut(s)	54, 241, 316, 319, 363
SspMI	CTAG	1 cut(s)	18
StuI	AGGCCT	1 cut(s)	263
StyD4I	CCNGG	2 cut(s)	46, 134
StyI	CCWWGG	3 cut(s)	93, 274, 375
TaaI	ACNGT	2 cut(s)	234, 486
TaqI	TCGA	1 cut(s)	266
TasI	AATT	2 cut(s)	225, 475
TauI	GCSGC	3 cut(s)	57, 318, 321
TfiI	GAWTC	1 cut(s)	88
Tru1I	TTAA	1 cut(s)	224
Tru9I	TTAA	1 cut(s)	224
TscAI	CASTG	1 cut(s)	239
TseI	GCWGC	1 cut(s)	243
TspDTI	ATGAA	1 cut(s)	464
TspRI	CASTG	1 cut(s)	239
VpaK11BI	GGWCC	1 cut(s)	10
XapI	RAATTY	1 cut(s)	475
XceI	RCATGY	1 cut(s)	346
XspI	CTAG	1 cut(s)	18

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.