MD01G1223900.v1.1

Thylakoid lumenal 17.4 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008800
Physical Location & Seq
Reverse (-)
31531356 .. 31534116
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1223900.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGCTGCTTCACTTTCTTCAGTGATTCCTCTGTCTCGAAACGGCGTTTCTCTCCAATTCTCCCACACGAAACGCCCGCAATTCCACTCCCCCACGCGCGTATTTTGCTCCGCTTCTAGGGATGGTCATGAAACAAAGGAAGAACTGAGTCAGTGGAAGCAAGCTAAGAATGTAGCTTGTGGGATTCTTGCTGTTTGGGCCGTGACTACCGCTGCCTCACCTGTAATTGCTGCTGGTCAGAGGTTGCCTCCACTGTCTACTGATCCAAAGCGATGTGAGGTTGCATTTGTTGGAAATACAATAGGTCAGGCAAATGGAGTTTACGACAAGGCAATTGATCTCCGCTTCTGTGACTACACAAACGAGAAGTCCAATCTTAAGGGAAAGAGTCTTGCGGCAGCACTCATGTCCGGAGCCAAATTTGATGGCGCAGACATGTCAGAAGTTGTGATGTCAAAGGCTTATGCTGCAGGAGCTAGCTTCAAGGGTACTGACTTCACGAATGCAGTTCTAGACCGGGTTAACTTTGAGAAAGCGAATCTGCAAGGAGCTTTGTTTGTTAACAGTGTCTTATCAGGTTCCACCTTTAACGAAGCAAACCTGGACGGTGCAGTTTTCGAGGATACCATTATAGGGTATATTGATCTTCAGAAAATTTGTAGAAATACATCTATCAATGACGAAGGAAGAGCTGCTCTAGGGTACTCCGTGGCCTTTGGATTGCATAAATCAAAGAAAAATCAAGCGACAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

252

Amino Acids

26.84

Weight (kDa)

8.54

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide PF00805 136 - 171 6.7e-07 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 151 - 186 1.2e-07 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 160 - 218 4.4e-08 Pentapeptide repeat region
Pentapeptide PF00805 170 - 208 7e-09 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 257
AccII CGCG 2 cut(s) 97, 99
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 5 cut(s) 77, 111, 210, 343, 395
AclWI GGATC 1 cut(s) 257
AcsI RAATTY 2 cut(s) 419, 654
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 2 cut(s) 490, 704
AfiI CCNNNNNNNGG 2 cut(s) 117, 633
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 484
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 6 cut(s) 164, 176, 476, 480, 551, 692
AluI AGCT 6 cut(s) 164, 176, 476, 480, 551, 692
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 2 cut(s) 198, 711
ApeKI GCWGC 6 cut(s) 5, 212, 230, 398, 467, 692
ApoI RAATTY 2 cut(s) 419, 654
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspLEI GCGC 2 cut(s) 99, 431
AspS9I GGNCC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 210
AsuNHI GCTAGC 1 cut(s) 476
BbvI GCAGC 5 cut(s) 199, 217, 410, 454, 679
BccI CCATC 2 cut(s) 116, 419
BceAI ACGGC 2 cut(s) 58, 185
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 1 cut(s) 616
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 4 cut(s) 117, 477, 512, 698
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 616
BisI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
BlsI GCNGC 7 cut(s) 7, 214, 232, 397, 400, 469, 694
Bme1390I CCNGG 2 cut(s) 518, 602
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 2 cut(s) 415, 580
BmrFI CCNGG 2 cut(s) 518, 602
BmtI GCTAGC 1 cut(s) 480
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpuMI CCSGG 1 cut(s) 518
BsaJI CCNNGG 1 cut(s) 708
BsaWI WCCGGW 1 cut(s) 410
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 633
BseAI TCCGGA 1 cut(s) 410
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 1 cut(s) 708
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 117, 633
BseMII CTCAG 1 cut(s) 137
BseXI GCAGC 5 cut(s) 199, 217, 410, 454, 679
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 2 cut(s) 97, 99
BshFI GGCC 2 cut(s) 200, 713
BsiSI CCGG 2 cut(s) 411, 517
BslI CCNNNNNNNGG 2 cut(s) 117, 633
BsmAI GTCTC 1 cut(s) 39
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 200, 713
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 3 cut(s) 262, 337, 643
BspACI CCGC 5 cut(s) 77, 111, 210, 343, 395
BspANI GGCC 2 cut(s) 200, 713
BspCNI CTCAG 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 2 cut(s) 97, 99
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 415, 580
BspMAI CTGCAG 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 682
BspTI CTTAAG 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 708
BssMI GATC 3 cut(s) 262, 337, 643
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 3 cut(s) 255, 566, 608
Bst6I CTCTTC 1 cut(s) 682
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 77, 162, 478
BstDEI CTNAG 2 cut(s) 146, 165
BstDSI CCRYGG 1 cut(s) 708
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 2 cut(s) 97, 99
BstHHI GCGC 2 cut(s) 99, 431
BstKTI GATC 3 cut(s) 265, 340, 646
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 3 cut(s) 262, 337, 643
BstMWI GCNNNNNNNGC 4 cut(s) 105, 197, 467, 473
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 2 cut(s) 516, 600
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 2 cut(s) 97, 99
BstV1I GCAGC 5 cut(s) 199, 217, 410, 454, 679
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 2 cut(s) 200, 713
BtgI CCRYGG 1 cut(s) 708
BtgZI GCGATG 1 cut(s) 286
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 4 cut(s) 27, 158, 251, 571
Cac8I GCNNGC 3 cut(s) 77, 162, 478
CciI TCATGA 1 cut(s) 127
CfoI GCGC 2 cut(s) 99, 431
Cfr13I GGNCC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 489, 703
CviAII CATG 3 cut(s) 128, 406, 436
CviQI GTAC 2 cut(s) 489, 703
DdeI CTNAG 2 cut(s) 146, 165
DpnI GATC 3 cut(s) 264, 339, 645
DpnII GATC 3 cut(s) 262, 337, 643
Eam1104I CTCTTC 1 cut(s) 682
EarI CTCTTC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 600
FaeI CATG 3 cut(s) 131, 409, 439
FaiI YATR 7 cut(s) 129, 407, 437, 465, 632, 639, 726
FatI CATG 3 cut(s) 127, 405, 435
FauI CCCGC 1 cut(s) 84
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
FspBI CTAG 4 cut(s) 117, 477, 512, 698
GlaI GCGC 2 cut(s) 98, 430
GluI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
HaeIII GGCC 2 cut(s) 200, 713
HapII CCGG 2 cut(s) 411, 517
HhaI GCGC 2 cut(s) 99, 431
Hin1II CATG 3 cut(s) 131, 409, 439
Hin6I GCGC 2 cut(s) 97, 429
HinP1I GCGC 2 cut(s) 97, 429
HincII GTYRAC 2 cut(s) 523, 562
HindII GTYRAC 2 cut(s) 523, 562
HinfI GANTC 5 cut(s) 25, 148, 184, 388, 538
HpaI GTTAAC 2 cut(s) 523, 562
HpaII CCGG 2 cut(s) 411, 517
HphI GGTGA 1 cut(s) 210
Hpy166II GTNNAC 4 cut(s) 258, 322, 523, 562
Hpy188I TCNGA 3 cut(s) 240, 442, 651
Hpy188III TCNNGA 5 cut(s) 36, 128, 411, 499, 512
Hpy8I GTNNAC 4 cut(s) 258, 322, 523, 562
HpyAV CCTTC 1 cut(s) 677
HpyCH4III ACNGT 3 cut(s) 255, 566, 608
HpyCH4V TGCA 6 cut(s) 284, 470, 506, 544, 611, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 197, 467, 473
HpyF3I CTNAG 2 cut(s) 146, 165
Hsp92II CATG 3 cut(s) 131, 409, 439
HspAI GCGC 2 cut(s) 97, 429
Kpn2I TCCGGA 1 cut(s) 410
KspAI GTTAAC 2 cut(s) 523, 562
Kzo9I GATC 3 cut(s) 262, 337, 643
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 4 cut(s) 113, 413, 473, 548
LpnPI CCDG 9 cut(s) 219, 234, 293, 424, 456, 530, 561, 587, 614
Lsp1109I GCAGC 5 cut(s) 199, 217, 410, 454, 679
MaeI CTAG 4 cut(s) 117, 477, 512, 698
MaeIII GTNAC 2 cut(s) 202, 350
MalI GATC 3 cut(s) 264, 339, 645
MboI GATC 3 cut(s) 262, 337, 643
MboII GAAGA 4 cut(s) 9, 152, 638, 699
MfeI CAATTG 1 cut(s) 333
MluCI AATT 6 cut(s) 56, 80, 225, 333, 419, 654
MlyI GAGTC 2 cut(s) 157, 397
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 6 cut(s) 39, 226, 234, 258, 271, 613
MroI TCCGGA 1 cut(s) 410
MseI TTAA 4 cut(s) 378, 522, 561, 588
MspA1I CMGCKG 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 411, 517
MspR9I CCNGG 2 cut(s) 518, 602
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 2 cut(s) 97, 99
MwoI GCNNNNNNNGC 4 cut(s) 105, 197, 467, 473
NciI CCSGG 1 cut(s) 518
NdeII GATC 3 cut(s) 262, 337, 643
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 3 cut(s) 131, 409, 439
NlaIV GGNNCC 2 cut(s) 415, 580
NmuCI GTSAC 2 cut(s) 202, 350
NspI RCATGY 1 cut(s) 439
PagI TCATGA 1 cut(s) 127
PciI ACATGT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 682
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 25, 184, 538
PkrI GCNGC 7 cut(s) 7, 214, 232, 397, 400, 469, 694
PleI GAGTC 2 cut(s) 156, 396
PpsI GAGTC 2 cut(s) 156, 396
PscI ACATGT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 2 cut(s) 415, 580
PspPI GGNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 472
RsaI GTAC 2 cut(s) 490, 704
RsaNI GTAC 2 cut(s) 489, 703
SapI GCTCTTC 1 cut(s) 682
SaqAI TTAA 4 cut(s) 378, 522, 561, 588
SatI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
Sau3AI GATC 3 cut(s) 262, 337, 643
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 2 cut(s) 157, 397
ScrFI CCNGG 2 cut(s) 518, 602
SfcI CTRYAG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 56, 80, 225, 333, 419, 654
SsiI CCGC 5 cut(s) 77, 111, 210, 343, 395
SspMI CTAG 4 cut(s) 117, 477, 512, 698
StyD4I CCNGG 2 cut(s) 516, 600
TaaI ACNGT 3 cut(s) 255, 566, 608
TaqI TCGA 2 cut(s) 37, 618
TasI AATT 6 cut(s) 56, 80, 225, 333, 419, 654
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 3 cut(s) 25, 184, 538
Tru1I TTAA 4 cut(s) 378, 522, 561, 588
Tru9I TTAA 4 cut(s) 378, 522, 561, 588
TscAI CASTG 4 cut(s) 27, 158, 258, 571
TseFI GTSAC 2 cut(s) 202, 350
TseI GCWGC 6 cut(s) 5, 212, 230, 398, 467, 692
Tsp45I GTSAC 2 cut(s) 202, 350
TspDTI ATGAA 1 cut(s) 144
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 4 cut(s) 27, 158, 258, 571
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 2 cut(s) 419, 654
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 4 cut(s) 117, 477, 512, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.