pycom01g23290

Thylakoid lumenal 17.4 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008800
Physical Location & Seq
Reverse (-)
20651460 .. 20653431
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23290.1

Sequence Viewer

Length: 711 bp
ATGGCTGCTTTACTTTCTTCAGCGATTCCTCTGTCTCGAAACGGCGTTTCTCTCCAATTCTCCCACACGAAACGCCCGCAATTCCACTCCCCCACGCGTGTATTTTGCTCCGCTTCTAGGAATGGTCATGAAACAAAGGAAGAACTGAGTCAGTGGAAGCAAGCTAAGAATGTAGCTTGTGGGATTCTTGCTGTTTGGGCCGTGACTACCGCTGCCTCACCTGTAATTGCTGCTGGTCAGAGGTTGCCTCCACTGTCTACTGATCCAAGGCGATGTGAGGTTGCATTTGTTGGAAATACGATAGGTCAGGCAAATGGAGTTTACGACAAGGCAATTGATCTCCGCTTCTGTGACTACACAAACGAGAAGTCCAATCTTAAGGGAAAGAGTCTTGCAGCAGCACTCATGTCCGGAGCCAAATTTGATGGGGCAGACATGTCAGAAGTTGTGATGTCGAAGGCTTATGCTGCAGGAGCTAGCTTCAAGGGTACCGACTTCACGAATGCAGTTCTAGACCGGGTTAACTTTGAGAAGGCGAATCTGCAAGGAGCTTTGTTTGTTAACAGTGTCTTATCAGGTTCCACCTTTAACGAAGCAAACCTTGACGGTGCAGTTTTCGAGGATACCATTATAGGGTACATTGATCTTCAGAAAATTTGTAGAAATACATCTATCAATGACGAAGGAAGAGCTGCTCTAGGGTGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

237

Amino Acids

25.23

Weight (kDa)

8.52

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide PF00805 136 - 171 6.1e-07 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 151 - 186 1.1e-07 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 160 - 218 4e-08 Pentapeptide repeat region
Pentapeptide PF00805 170 - 208 6.3e-09 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AccI GTMKAC 1 cut(s) 257
AccII CGCG 1 cut(s) 97
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 4 cut(s) 77, 111, 210, 343
AclWI GGATC 1 cut(s) 257
AcsI RAATTY 2 cut(s) 419, 654
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 2 cut(s) 490, 638
AfiI CCNNNNNNNGG 2 cut(s) 117, 633
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 2 cut(s) 95, 435
AgsI TTSAA 1 cut(s) 484
AluBI AGCT 6 cut(s) 164, 176, 476, 480, 551, 692
AluI AGCT 6 cut(s) 164, 176, 476, 480, 551, 692
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 7 cut(s) 5, 212, 230, 395, 398, 467, 692
ApoI RAATTY 2 cut(s) 419, 654
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
Asp718I GGTACC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 210
AsuNHI GCTAGC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 488
BbvI GCAGC 6 cut(s) 199, 217, 407, 410, 454, 679
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 58, 185
BciVI GTATCC 1 cut(s) 616
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 4 cut(s) 117, 477, 512, 698
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 616
BisI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
BlsI GCNGC 7 cut(s) 7, 214, 232, 397, 400, 469, 694
Bme1390I CCNGG 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 3 cut(s) 415, 490, 580
BmrFI CCNGG 1 cut(s) 518
BmtI GCTAGC 1 cut(s) 480
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpuMI CCSGG 1 cut(s) 518
BsaJI CCNNGG 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 410
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 633
BseAI TCCGGA 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 266
BseLI CCNNNNNNNGG 2 cut(s) 117, 633
BseMII CTCAG 1 cut(s) 137
BseXI GCAGC 6 cut(s) 199, 217, 407, 410, 454, 679
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 488
BsiSI CCGG 2 cut(s) 411, 517
BslI CCNNNNNNNGG 2 cut(s) 117, 633
BsmAI GTCTC 1 cut(s) 39
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 3 cut(s) 262, 337, 643
BspACI CCGC 4 cut(s) 77, 111, 210, 343
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 1 cut(s) 97
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 3 cut(s) 415, 490, 580
BspMAI CTGCAG 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 682
BspT107I GGYRCC 1 cut(s) 488
BspTI CTTAAG 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 266
BssMI GATC 3 cut(s) 262, 337, 643
BssT1I CCWWGG 1 cut(s) 266
Bst4CI ACNGT 3 cut(s) 255, 566, 608
Bst6I CTCTTC 1 cut(s) 682
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 77, 162, 478
BstDEI CTNAG 2 cut(s) 146, 165
BstFNI CGCG 1 cut(s) 97
BstKTI GATC 3 cut(s) 265, 340, 646
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 3 cut(s) 262, 337, 643
BstMWI GCNNNNNNNGC 3 cut(s) 197, 467, 473
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 6 cut(s) 199, 217, 407, 410, 454, 679
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 286
BtsIMutI CAGTG 3 cut(s) 158, 251, 571
Cac8I GCNNGC 3 cut(s) 77, 162, 478
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 489, 637
CviAII CATG 3 cut(s) 128, 406, 436
CviQI GTAC 2 cut(s) 489, 637
DdeI CTNAG 2 cut(s) 146, 165
DpnI GATC 3 cut(s) 264, 339, 645
DpnII GATC 3 cut(s) 262, 337, 643
Eam1104I CTCTTC 1 cut(s) 682
EarI CTCTTC 1 cut(s) 682
Eco130I CCWWGG 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 632
EcoT14I CCWWGG 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 266
FaeI CATG 3 cut(s) 131, 409, 439
FaiI YATR 5 cut(s) 129, 407, 437, 465, 632
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FatI CATG 3 cut(s) 127, 405, 435
FauI CCCGC 1 cut(s) 84
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
Fsp4HI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
FspBI CTAG 4 cut(s) 117, 477, 512, 698
GluI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
HaeIII GGCC 1 cut(s) 200
HapII CCGG 2 cut(s) 411, 517
Hin1II CATG 3 cut(s) 131, 409, 439
HincII GTYRAC 2 cut(s) 523, 562
HindII GTYRAC 2 cut(s) 523, 562
HinfI GANTC 5 cut(s) 25, 148, 184, 388, 538
HpaI GTTAAC 2 cut(s) 523, 562
HpaII CCGG 2 cut(s) 411, 517
HphI GGTGA 1 cut(s) 210
Hpy166II GTNNAC 4 cut(s) 258, 322, 523, 562
Hpy188I TCNGA 3 cut(s) 240, 442, 651
Hpy188III TCNNGA 5 cut(s) 36, 128, 411, 499, 512
Hpy8I GTNNAC 4 cut(s) 258, 322, 523, 562
HpyAV CCTTC 3 cut(s) 451, 526, 677
HpyCH4III ACNGT 3 cut(s) 255, 566, 608
HpyCH4V TGCA 6 cut(s) 284, 395, 470, 506, 544, 611
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 467, 473
HpyF3I CTNAG 2 cut(s) 146, 165
Hsp92II CATG 3 cut(s) 131, 409, 439
Kpn2I TCCGGA 1 cut(s) 410
KpnI GGTACC 1 cut(s) 492
KspAI GTTAAC 2 cut(s) 523, 562
Kzo9I GATC 3 cut(s) 262, 337, 643
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 4 cut(s) 113, 413, 473, 548
LpnPI CCDG 7 cut(s) 219, 234, 293, 424, 456, 530, 561
Lsp1109I GCAGC 6 cut(s) 199, 217, 407, 410, 454, 679
MaeI CTAG 4 cut(s) 117, 477, 512, 698
MaeIII GTNAC 2 cut(s) 202, 350
MalI GATC 3 cut(s) 264, 339, 645
MboI GATC 3 cut(s) 262, 337, 643
MboII GAAGA 4 cut(s) 9, 152, 638, 699
MfeI CAATTG 1 cut(s) 333
MluCI AATT 6 cut(s) 56, 80, 225, 333, 419, 654
MluI ACGCGT 1 cut(s) 95
MlyI GAGTC 2 cut(s) 157, 397
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 6 cut(s) 39, 226, 234, 258, 271, 613
MroI TCCGGA 1 cut(s) 410
MseI TTAA 4 cut(s) 378, 522, 561, 588
MspA1I CMGCKG 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 411, 517
MspR9I CCNGG 1 cut(s) 518
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 508
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 3 cut(s) 197, 467, 473
NciI CCSGG 1 cut(s) 518
NdeII GATC 3 cut(s) 262, 337, 643
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 3 cut(s) 131, 409, 439
NlaIV GGNNCC 3 cut(s) 415, 490, 580
NmuCI GTSAC 2 cut(s) 202, 350
NspI RCATGY 1 cut(s) 439
PagI TCATGA 1 cut(s) 127
PciI ACATGT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 682
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 25, 184, 538
PkrI GCNGC 7 cut(s) 7, 214, 232, 397, 400, 469, 694
PleI GAGTC 2 cut(s) 156, 396
PpsI GAGTC 2 cut(s) 156, 396
PscI ACATGT 1 cut(s) 435
PspN4I GGNNCC 3 cut(s) 415, 490, 580
PspPI GGNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 472
RsaI GTAC 2 cut(s) 490, 638
RsaNI GTAC 2 cut(s) 489, 637
SapI GCTCTTC 1 cut(s) 682
SaqAI TTAA 4 cut(s) 378, 522, 561, 588
SatI GCNGC 7 cut(s) 6, 213, 231, 396, 399, 468, 693
Sau3AI GATC 3 cut(s) 262, 337, 643
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 2 cut(s) 157, 397
ScrFI CCNGG 1 cut(s) 518
SfcI CTRYAG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 56, 80, 225, 333, 419, 654
SsiI CCGC 4 cut(s) 77, 111, 210, 343
SspMI CTAG 4 cut(s) 117, 477, 512, 698
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 1 cut(s) 266
TaaI ACNGT 3 cut(s) 255, 566, 608
TaqI TCGA 4 cut(s) 37, 455, 618, 706
TasI AATT 6 cut(s) 56, 80, 225, 333, 419, 654
TfiI GAWTC 3 cut(s) 25, 184, 538
Tru1I TTAA 4 cut(s) 378, 522, 561, 588
Tru9I TTAA 4 cut(s) 378, 522, 561, 588
TscAI CASTG 3 cut(s) 158, 258, 571
TseFI GTSAC 2 cut(s) 202, 350
TseI GCWGC 7 cut(s) 5, 212, 230, 395, 398, 467, 692
Tsp45I GTSAC 2 cut(s) 202, 350
TspDTI ATGAA 1 cut(s) 144
TspRI CASTG 3 cut(s) 158, 258, 571
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 2 cut(s) 419, 654
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 4 cut(s) 117, 477, 512, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.