Rw1G039600

Thylakoid lumenal 17.4 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008800
Physical Location & Seq
Reverse (-)
67125408 .. 67127169
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G039600.1

Sequence Viewer

Length: 702 bp
ATGGCTGCTTCTCTTTCAATTCCATTGTCGACCCGGTTCTCTCCAGCGAAACGTCATCGTTTTAATGCCTTGGAGTTCCGTTCACCTCTGCGTGTTAGTTGCTCTGCTTCTAGGGATGGTCCTGAATTGGAGGGAAAGTTACCTCAGTTGAAGCAGCTAAAGAATGTAGCTTGTGGCATTCTTGCTGTTTGGGCAGTAACTACTGCCTCACCAGTAATTGCTGCTAATCAGAGGCTGCCTCCACTGTCAACTGACCCAAAAAGATGTGAGCTTGCATTTGTTGGTAACACGATAGGTCAAGCAAATGGAGTTTATGACAAGGCAATTGATCTCCGCTTCTGTGATTTTACAAATGAAAAGGCCAACCTGAAGGGGAAGAGTCTTGCAGCAGCACTCATGTCTGAGGCAAAGTTTGATGGTGCAGACATGACAGAAGTTGTAATGTCAAAGGCTTATGCCGCAGGAGCTAGCTTCAAGGGTACAGACTTCTCGAATGCAGTTCTAGACAGGGTTAACTTTGAGAAAGCTAATCTACAAGGAGCTTTATTTATAAACACTGTATTATCAGGCTCCACTTTCAATGATGCACAACTTGATGGTGCAGTGTTTGAGGACACCATTATAGGATACATTGATCTTCAGAAACTTTGTAGAAATACAAGTATCAACGAAGAAGGAAGAGATGTTCTGGGGTGCCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pentapeptide

Protein Analysis

233

Amino Acids

25.1

Weight (kDa)

8.19

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide PF00805 133 - 168 1.5e-06 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 148 - 183 8.4e-08 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 157 - 214 4.8e-07 Pentapeptide repeat region
Pentapeptide PF00805 167 - 205 6e-09 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
AccB1I GGYRCC 1 cut(s) 693
AccI GTMKAC 1 cut(s) 29
AciI CCGC 2 cut(s) 334, 459
AcuI CTGAAG 2 cut(s) 389, 623
AfaI GTAC 1 cut(s) 481
AgsI TTSAA 4 cut(s) 18, 151, 475, 580
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 7 cut(s) 157, 170, 271, 467, 471, 527, 542
AluI AGCT 7 cut(s) 157, 170, 271, 467, 471, 527, 542
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 6 cut(s) 5, 154, 221, 235, 386, 389
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 75, 201
AsuNHI GCTAGC 1 cut(s) 467
AvaII GGWCC 1 cut(s) 119
BanI GGYRCC 1 cut(s) 693
BbvI GCAGC 5 cut(s) 166, 208, 222, 398, 401
BccI CCATC 3 cut(s) 110, 410, 590
BciVI GTATCC 1 cut(s) 620
BcnI CCSGG 1 cut(s) 34
BfaI CTAG 3 cut(s) 111, 468, 503
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 7 cut(s) 6, 155, 222, 236, 387, 390, 459
BlsI GCNGC 7 cut(s) 7, 156, 223, 237, 388, 391, 460
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 571, 695
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 574
BmtI GCTAGC 1 cut(s) 471
BplI GAGNNNNNCTC 2 cut(s) 223, 255
BpmI CTGGAG 1 cut(s) 27
BpuMI CCSGG 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 158, 393
BseNI ACTGG 1 cut(s) 212
BseXI GCAGC 5 cut(s) 166, 208, 222, 398, 401
BsgI GTGCAG 2 cut(s) 441, 621
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 1 cut(s) 693
BsiSI CCGG 1 cut(s) 34
BsmI GAATGC 2 cut(s) 177, 499
BsnI GGCC 1 cut(s) 362
Bsp143I GATC 2 cut(s) 328, 634
BspACI CCGC 2 cut(s) 334, 459
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 2 cut(s) 157, 394
BspLI GGNNCC 2 cut(s) 571, 695
BspOI GCTAGC 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 693
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 328, 634
BssT1I CCWWGG 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 246, 559
Bst6I CTCTTC 2 cut(s) 371, 673
BstC8I GCNNGC 2 cut(s) 273, 469
BstDEI CTNAG 2 cut(s) 144, 402
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 331, 637
BstMBI GATC 2 cut(s) 328, 634
BstMWI GCNNNNNNNGC 3 cut(s) 191, 458, 464
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 5 cut(s) 166, 208, 222, 398, 401
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 242, 555, 609
Cac8I GCNNGC 2 cut(s) 273, 469
CaiI CAGNNNCTG 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 480
CviAII CATG 2 cut(s) 397, 427
CviQI GTAC 1 cut(s) 480
DdeI CTNAG 2 cut(s) 144, 402
DpnI GATC 2 cut(s) 330, 636
DpnII GATC 2 cut(s) 328, 634
Eam1104I CTCTTC 2 cut(s) 371, 673
EarI CTCTTC 2 cut(s) 371, 673
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 119
Eco57I CTGAAG 2 cut(s) 389, 623
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 2 cut(s) 400, 430
FaiI YATR 6 cut(s) 315, 398, 428, 456, 551, 623
FatI CATG 2 cut(s) 396, 426
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 7 cut(s) 6, 155, 222, 236, 387, 390, 459
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 7 cut(s) 6, 155, 222, 236, 387, 390, 459
FspBI CTAG 3 cut(s) 111, 468, 503
GluI GCNGC 7 cut(s) 6, 155, 222, 236, 387, 390, 459
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 362
HapII CCGG 1 cut(s) 34
Hin1II CATG 2 cut(s) 400, 430
HincII GTYRAC 3 cut(s) 30, 249, 514
HindII GTYRAC 3 cut(s) 30, 249, 514
HinfI GANTC 1 cut(s) 379
HpaI GTTAAC 1 cut(s) 514
HpaII CCGG 1 cut(s) 34
HphI GGTGA 2 cut(s) 75, 201
Hpy166II GTNNAC 4 cut(s) 30, 83, 249, 514
Hpy188I TCNGA 3 cut(s) 231, 403, 642
Hpy188III TCNNGA 3 cut(s) 122, 490, 503
Hpy8I GTNNAC 4 cut(s) 30, 83, 249, 514
HpyAV CCTTC 2 cut(s) 364, 668
HpyCH4III ACNGT 2 cut(s) 246, 559
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 6 cut(s) 275, 386, 422, 497, 587, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 458, 464
HpyF3I CTNAG 2 cut(s) 144, 402
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 2 cut(s) 400, 430
KspAI GTTAAC 1 cut(s) 514
Kzo9I GATC 2 cut(s) 328, 634
LmnI GCTCC 3 cut(s) 464, 539, 575
LpnPI CCDG 9 cut(s) 47, 57, 135, 225, 380, 447, 493, 552, 674
Lsp1109I GCAGC 5 cut(s) 166, 208, 222, 398, 401
LweI GCATC 1 cut(s) 574
MaeI CTAG 3 cut(s) 111, 468, 503
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 3 cut(s) 138, 196, 284
MalI GATC 2 cut(s) 330, 636
MboI GATC 2 cut(s) 328, 634
MboII GAAGA 4 cut(s) 388, 629, 683, 690
MfeI CAATTG 1 cut(s) 324
MluCI AATT 4 cut(s) 18, 125, 216, 324
MlyI GAGTC 1 cut(s) 388
MnlI CCTC 8 cut(s) 96, 124, 153, 217, 225, 249, 397, 604
MseI TTAA 2 cut(s) 63, 513
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 34
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 2 cut(s) 177, 499
MwoI GCNNNNNNNGC 3 cut(s) 191, 458, 464
NciI CCSGG 1 cut(s) 34
NdeII GATC 2 cut(s) 328, 634
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 2 cut(s) 400, 430
NlaIV GGNNCC 2 cut(s) 571, 695
PctI GAATGC 2 cut(s) 177, 499
PkrI GCNGC 7 cut(s) 7, 156, 223, 237, 388, 391, 460
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
PsiI TTATAA 1 cut(s) 551
PspN4I GGNNCC 2 cut(s) 571, 695
PspPI GGNCC 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 2 cut(s) 63, 513
SatI GCNGC 7 cut(s) 6, 155, 222, 236, 387, 390, 459
Sau3AI GATC 2 cut(s) 328, 634
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 34
SfaNI GCATC 1 cut(s) 574
SinI GGWCC 1 cut(s) 119
Sse9I AATT 4 cut(s) 18, 125, 216, 324
SsiI CCGC 2 cut(s) 334, 459
SspMI CTAG 3 cut(s) 111, 468, 503
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 246, 559
TaiI ACGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 29, 491
TasI AATT 4 cut(s) 18, 125, 216, 324
TauI GCSGC 1 cut(s) 461
Tru1I TTAA 2 cut(s) 63, 513
Tru9I TTAA 2 cut(s) 63, 513
TscAI CASTG 3 cut(s) 249, 562, 609
TseI GCWGC 6 cut(s) 5, 154, 221, 235, 386, 389
TspDTI ATGAA 1 cut(s) 369
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 3 cut(s) 249, 562, 609
VpaK11BI GGWCC 1 cut(s) 119
XbaI TCTAGA 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 3 cut(s) 111, 468, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.