MD01G1227100.v1.1

Auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011864
Physical Location & Seq
Forward (+)
31700591 .. 31700992
402 bp
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UTR
Exon/CDS
Intron
MD01G1227100.v1.1.491

Sequence Viewer

Length: 402 bp
ATGGGATTTATCGCCGCCGACAGGCCCAGCAAGCACAACCACCTGAACTTCCACCTCCACTTCCCCCACCTTCTCCACCACCACCACGACAAGAACAGCAAGGAGCTCAAAGATATTCCGAAAGGCTGTATGGCTGTGATGGTGGGCCAGGGGAAGGACCAGCAGAGGTTTGTTATTCCGGTGGTCTACATCAACCACCCGCTCTTCATGCAGCTGCTGAAGGAGGCGGAGAAGGAGTACGGATTCGATCACAAAGGTCCGATCACTATTCCTTGCCATGTGGAGGAGTTTCGCACCGTTCAAGGCATGATCGATAGGGAGACTTCGTTCCACGGCCATCATCATCATCATCACCATCACAACCACCACCATCACCATTATGCCTGGTGTTTTAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

134

Amino Acids

15.84

Weight (kDa)

7.97

Isoelectric Point (pI)

24.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 29 - 103 9.6e-23 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 186
AciI CCGC 3 cut(s) 15, 200, 227
AcoI YGGCCR 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 239
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 21, 154, 283
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 2 cut(s) 147, 383
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
AluBI AGCT 2 cut(s) 106, 214
AluI AGCT 2 cut(s) 106, 214
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 3 cut(s) 23, 145, 334
ApeKI GCWGC 2 cut(s) 211, 214
AspS9I GGNCC 4 cut(s) 24, 145, 157, 257
AsuHPI GGTGA 2 cut(s) 344, 365
AvaII GGWCC 2 cut(s) 157, 257
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 201, 223
BccI CCATC 4 cut(s) 133, 345, 363, 378
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 2 cut(s) 149, 385
BcoDI GTCTC 1 cut(s) 314
BisI GCNGC 3 cut(s) 15, 212, 215
BlsI GCNGC 3 cut(s) 16, 213, 216
Bme1390I CCNGG 2 cut(s) 149, 385
Bme18I GGWCC 2 cut(s) 157, 257
BmgT120I GGNCC 4 cut(s) 24, 145, 157, 257
BmrFI CCNGG 2 cut(s) 149, 385
Bsa29I ATCGAT 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 148, 331
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 311, 341
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 154, 283
BseBI CCWGG 2 cut(s) 149, 385
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 148, 331
BseLI CCNNNNNNNGG 3 cut(s) 21, 154, 283
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 2 cut(s) 201, 223
BseYI CCCAGC 1 cut(s) 26
BshFI GGCC 3 cut(s) 25, 147, 336
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 3 cut(s) 21, 154, 283
BsmAI GTCTC 1 cut(s) 314
BsnI GGCC 3 cut(s) 25, 147, 336
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 3 cut(s) 247, 261, 309
BspACI CCGC 3 cut(s) 15, 200, 227
BspANI GGCC 3 cut(s) 25, 147, 336
BspDI ATCGAT 1 cut(s) 312
BspQI GCTCTTC 1 cut(s) 209
BsrBI CCGCTC 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 148, 331
BssMI GATC 3 cut(s) 247, 261, 309
Bst2UI CCWGG 2 cut(s) 149, 385
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 331
BstKTI GATC 3 cut(s) 250, 264, 312
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 3 cut(s) 247, 261, 309
BstMWI GCNNNNNNNGC 2 cut(s) 31, 208
BstNI CCWGG 2 cut(s) 149, 385
BstSCI CCNGG 2 cut(s) 147, 383
BstV1I GCAGC 2 cut(s) 201, 223
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 3 cut(s) 25, 147, 336
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 32
CaiI CAGNNNCTG 1 cut(s) 217
Cfr13I GGNCC 4 cut(s) 24, 145, 157, 257
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 208, 278, 307
CviJI RGCY 7 cut(s) 25, 106, 126, 134, 147, 214, 336
CviKI_1 RGCY 7 cut(s) 25, 106, 126, 134, 147, 214, 336
CviQI GTAC 1 cut(s) 238
DpnI GATC 3 cut(s) 249, 263, 311
DpnII GATC 3 cut(s) 247, 261, 309
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
EciI GGCGGA 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 157, 257
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 147, 383
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 3 cut(s) 211, 281, 310
FaiI YATR 5 cut(s) 131, 209, 279, 308, 381
FatI CATG 3 cut(s) 207, 277, 306
FauI CCCGC 1 cut(s) 207
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 15, 212, 215
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 3 cut(s) 15, 212, 215
GluI GCNGC 3 cut(s) 15, 212, 215
GsaI CCCAGC 1 cut(s) 30
HaeIII GGCC 3 cut(s) 25, 147, 336
HapII CCGG 1 cut(s) 179
Hin1II CATG 3 cut(s) 211, 281, 310
HinfI GANTC 1 cut(s) 243
HpaII CCGG 1 cut(s) 179
HphI GGTGA 2 cut(s) 344, 365
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 120, 261
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 80, 148, 214, 226
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 208
Hsp92II CATG 3 cut(s) 211, 281, 310
Kzo9I GATC 3 cut(s) 247, 261, 309
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 9 cut(s) 7, 40, 56, 134, 161, 173, 192, 370, 397
Lsp1109I GCAGC 2 cut(s) 201, 223
MalI GATC 3 cut(s) 249, 263, 311
MbiI CCGCTC 1 cut(s) 202
MboI GATC 3 cut(s) 247, 261, 309
MboII GAAGA 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 108
MnlI CCTC 4 cut(s) 65, 159, 217, 277
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 149, 385
MvaI CCWGG 2 cut(s) 149, 385
MwoI GCNNNNNNNGC 2 cut(s) 31, 208
NdeII GATC 3 cut(s) 247, 261, 309
NlaIII CATG 3 cut(s) 211, 281, 310
PciSI GCTCTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 243
PkrI GCNGC 3 cut(s) 16, 213, 216
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 147, 383
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 147, 383
PspPI GGNCC 4 cut(s) 24, 145, 157, 257
PstNI CAGNNNCTG 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 209
SatI GCNGC 3 cut(s) 15, 212, 215
Sau3AI GATC 3 cut(s) 247, 261, 309
Sau96I GGNCC 4 cut(s) 24, 145, 157, 257
ScrFI CCNGG 2 cut(s) 149, 385
SduI GDGCHC 1 cut(s) 108
SetI ASST 7 cut(s) 45, 57, 72, 108, 170, 216, 259
SinI GGWCC 2 cut(s) 157, 257
SmiMI CAYNNNNRTG 1 cut(s) 378
SsiI CCGC 3 cut(s) 15, 200, 227
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 147, 383
TaaI ACNGT 1 cut(s) 298
TaqI TCGA 2 cut(s) 246, 312
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 243
TseI GCWGC 2 cut(s) 211, 214
TspDTI ATGAA 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 157, 257
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.