Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007146

Physical Location & Seq

Genomic Coordinates Reverse (-)

31892842 .. 31895877

Gene Structure 3036 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1229700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGGGTGAAGACGCTAAGCAGGAACAAGCTAAGGCAGAGCCTAAGCCAGAGGAGAAGGCCGAGGCGAAAGTAGAGGAAAAGAAAGAAGAAGAGGAGAAAAAGGAAGAGCCAAAGCCTCCTTCTCCCTTCGTGTTGTTTGTAAACTTGCACTGTGTGGGATGTGCAAAGAAGATTGAGAGGTCCATCATGAGAATTAGAGGAGTGGAGGGGGTTGTGATAGATATGGCTAAAAATGAAGTGACCATAAAGGGAATAGTGGAGCCTCAGGCAGTGTGCAACGAAATCTTGAAGAAAACCAAGAGAAAAGCAAAAGTCTTGTCCCCATTACCAGCTGCTGAGGGTGAACCCATTCCAGAAGTTGTTGCCTCACAGGTTAGTGGACTTGTAACTGTAGAACTCGAAGTTAACATGCACTGTGAGGCCTGTGCAGAGCAACTCAAGAAAAAGATACTCAAACTCAGAGGAGTGCAAAGTGCTGTGACTGATCATAGTAGCGGGAAGGTAATGGTGACAGGGACCATGGACGGGGACAAGCTAGTAGAGTATGTGTATAGACGAACCAAGAAGCAAGCCCGAATAGTCCCACAACCCGAGCCCGAACCCGAGAAGAAAGAAGATGAGAAGCCAGCTGCAGAAGAAGCAAAACCCGAAGAGGAGAAGAAAGAAGAAAATGCAGGGAAAAAAGAAGAGGATAAACCGGCAGAAGGGGAGGGAAAGAAAGAAGAGGGCGGAGCTGACGGGGGAGAAAGTAATAATAAGGAGGAGGAGAAAGGTGGCCAGGAGAACAAAGAAGAGGGGAAGAAGGTGGGAGAGATGATGAGTGGCAGTTACGTAAATAGTGGCATGGATGAGGAACAGATGAAAAGAATGATGCAGTACTATTATCAACCTCTTTTCGTGATTGAGAGAATCCCACCTCCTCAGCTGTTCAGTGATGAGAATCCCAATGCGTGTTGCATTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko04978 map04978

Pfam Domains

Protein Families

HMA

Protein Analysis

322

Amino Acids

35.71

Weight (kDa)

5.14

Isoelectric Point (pI)

60.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	45 - 99	4.4e-11	Heavy-metal-associated domain
HMA	PF00403	135 - 180	1.2e-08	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007146)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24580 AT5G24580 AT5G24580 AT5G24580 AT5G24580
fragaria_vesca	FvH4_7g33060
malus_domestica	MD01G1229700.v1.1 MD07G1301700.v1.1
prunus_persica	Prupe.2G320300_v2.0.a1
pyrus_communis	pycom01g23780 pycom07g27450
rosa_chinensis	RchiOBHm_Chr1g0330391 RchiOBHm_Chr1g0383071
rosa_laevigata	RLG00000026135
rosa_multiflora	Rmu_sc0000634.1_g000006 Rmu_sc0008563.1_g000016 Rmu_sc0033623.1_g000002
rosa_roxburghii	Rroxscaffold_4G00277640
rosa_rugosa	Rorug01G0438100
rosa_samantha	Rh1AG463800 Rh1BG421000 Rh1CG434500 Rh1DG453600
rosa_wichuraiana	Rw1G008460 Rw1G040840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	495, 729
AcoI	YGGCCR	1 cut(s)	775
AdeI	CACNNNGTG	1 cut(s)	154
AfaI	GTAC	1 cut(s)	878
AfiI	CCNNNNNNNGG	2 cut(s)	525, 704
AgsI	TTSAA	1 cut(s)	289
AjnI	CCWGG	1 cut(s)	777
AjuI	GAANNNNNNNTTGG	2 cut(s)	103, 135
AluBI	AGCT	6 cut(s)	29, 332, 535, 629, 734, 925
AluI	AGCT	6 cut(s)	29, 332, 535, 629, 734, 925
AlwNI	CAGNNNCTG	1 cut(s)	335
Ama87I	CYCGRG	2 cut(s)	590, 602
AoxI	GGCC	3 cut(s)	57, 420, 775
ApeKI	GCWGC	2 cut(s)	332, 629
ArsI	GACNNNNNNTTYG	2 cut(s)	302, 334
Asp700I	GAANNNNTTC	1 cut(s)	348
AspS9I	GGNCC	2 cut(s)	180, 516
AsuHPI	GGTGA	3 cut(s)	17, 353, 520
AvaI	CYCGRG	2 cut(s)	590, 602
AvaII	GGWCC	2 cut(s)	180, 516
AxyI	CCTNAGG	1 cut(s)	264
BalI	TGGCCA	1 cut(s)	777
BanII	GRGCYC	1 cut(s)	597
BbsI	GAAGAC	1 cut(s)	15
BbvCI	CCTCAGC	2 cut(s)	336, 921
BbvI	GCAGC	2 cut(s)	319, 616
BccI	CCATC	1 cut(s)	191
BciT130I	CCWGG	1 cut(s)	779
BclI	TGATCA	1 cut(s)	484
BfaI	CTAG	1 cut(s)	536
BfmI	CTRYAG	2 cut(s)	390, 630
BisI	GCNGC	2 cut(s)	333, 630
BlpI	GCTNAGC	1 cut(s)	15
BlsI	GCNGC	2 cut(s)	334, 631
BmcAI	AGTACT	1 cut(s)	878
Bme1390I	CCNGG	1 cut(s)	779
Bme18I	GGWCC	2 cut(s)	180, 516
BmeT110I	CYCGRG	2 cut(s)	590, 602
BmgT120I	GGNCC	2 cut(s)	180, 516
BmiI	GGNNCC	2 cut(s)	261, 517
BmrFI	CCNGG	1 cut(s)	779
BmsI	GCATC	1 cut(s)	861
BpiI	GAAGAC	1 cut(s)	15
Bpu10I	CCTNAGC	4 cut(s)	30, 42, 336, 921
Bpu1102I	GCTNAGC	1 cut(s)	15
BpuEI	CTTGAG	1 cut(s)	422
BsaAI	YACGTR	1 cut(s)	832
BsaBI	GATNNNNATC	1 cut(s)	939
BsaJI	CCNNGG	2 cut(s)	60, 519
Bsc4I	CCNNNNNNNGG	2 cut(s)	525, 704
Bse118I	RCCGGY	1 cut(s)	697
Bse21I	CCTNAGG	1 cut(s)	264
Bse8I	GATNNNNATC	1 cut(s)	939
BseBI	CCWGG	1 cut(s)	779
BseDI	CCNNGG	2 cut(s)	60, 519
BseGI	GGATG	2 cut(s)	164, 853
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	2 cut(s)	525, 704
BseMII	CTCAG	4 cut(s)	278, 327, 472, 935
BseRI	GAGGAG	8 cut(s)	65, 107, 213, 477, 668, 776, 779, 909
BseXI	GCAGC	2 cut(s)	319, 616
BsgI	GTGCAG	1 cut(s)	447
BshFI	GGCC	3 cut(s)	59, 422, 777
BsiHKCI	CYCGRG	2 cut(s)	590, 602
BsiSI	CCGG	1 cut(s)	698
BslFI	GGGAC	4 cut(s)	304, 529, 542, 566
BslI	CCNNNNNNNGG	2 cut(s)	525, 704
BsmFI	GGGAC	4 cut(s)	304, 529, 542, 566
BsnI	GGCC	3 cut(s)	59, 422, 777
BsoBI	CYCGRG	2 cut(s)	590, 602
Bsp1286I	GDGCHC	1 cut(s)	597
Bsp143I	GATC	1 cut(s)	484
Bsp1720I	GCTNAGC	1 cut(s)	15
Bsp19I	CCATGG	1 cut(s)	519
BspACI	CCGC	2 cut(s)	495, 729
BspANI	GGCC	3 cut(s)	59, 422, 777
BspCNI	CTCAG	4 cut(s)	277, 328, 471, 934
BspHI	TCATGA	2 cut(s)	186, 962
BspLI	GGNNCC	2 cut(s)	261, 517
BspMAI	CTGCAG	1 cut(s)	634
BspQI	GCTCTTC	1 cut(s)	99
BsrFI	RCCGGY	1 cut(s)	697
BssAI	RCCGGY	1 cut(s)	697
BssECI	CCNNGG	2 cut(s)	60, 519
BssMI	GATC	1 cut(s)	484
BssT1I	CCWWGG	1 cut(s)	519
Bst2UI	CCWGG	1 cut(s)	779
Bst4CI	ACNGT	3 cut(s)	152, 391, 416
Bst6I	CTCTTC	6 cut(s)	84, 99, 645, 681, 717, 786
BstBAI	YACGTR	1 cut(s)	832
BstC8I	GCNNGC	2 cut(s)	570, 627
BstDEI	CTNAG	7 cut(s)	15, 30, 42, 264, 336, 458, 921
BstDSI	CCRYGG	1 cut(s)	519
BstF5I	GGATG	2 cut(s)	164, 853
BstKTI	GATC	1 cut(s)	487
BstMBI	GATC	1 cut(s)	484
BstMWI	GCNNNNNNNGC	1 cut(s)	638
BstNI	CCWGG	1 cut(s)	779
BstNSI	RCATGY	1 cut(s)	412
BstSCI	CCNGG	1 cut(s)	777
BstSFI	CTRYAG	2 cut(s)	390, 630
BstSNI	TACGTA	1 cut(s)	832
BstV1I	GCAGC	2 cut(s)	319, 616
BstV2I	GAAGAC	1 cut(s)	15
Bsu36I	CCTNAGG	1 cut(s)	264
BsuRI	GGCC	3 cut(s)	59, 422, 777
BtgI	CCRYGG	1 cut(s)	519
BtsCI	GGATG	2 cut(s)	164, 853
BtsI	GCAGTG	1 cut(s)	276
BtsIMutI	CAGTG	4 cut(s)	148, 276, 412, 937
Cac8I	GCNNGC	2 cut(s)	570, 627
CaiI	CAGNNNCTG	1 cut(s)	335
CciI	TCATGA	2 cut(s)	186, 962
Cfr10I	RCCGGY	1 cut(s)	697
Cfr13I	GGNCC	2 cut(s)	180, 516
CseI	GACGC	1 cut(s)	20
Csp6I	GTAC	1 cut(s)	877
CviAII	CATG	5 cut(s)	187, 409, 520, 844, 963
CviQI	GTAC	1 cut(s)	877
DdeI	CTNAG	7 cut(s)	15, 30, 42, 264, 336, 458, 921
DpnI	GATC	1 cut(s)	486
DpnII	GATC	1 cut(s)	484
DraIII	CACNNNGTG	1 cut(s)	154
EaeI	YGGCCR	1 cut(s)	775
Eam1104I	CTCTTC	6 cut(s)	84, 99, 645, 681, 717, 786
EarI	CTCTTC	6 cut(s)	84, 99, 645, 681, 717, 786
EciI	GGCGGA	1 cut(s)	744
Eco105I	TACGTA	1 cut(s)	832
Eco130I	CCWWGG	1 cut(s)	519
Eco147I	AGGCCT	1 cut(s)	422
Eco24I	GRGCYC	1 cut(s)	597
Eco47I	GGWCC	2 cut(s)	180, 516
Eco81I	CCTNAGG	1 cut(s)	264
Eco88I	CYCGRG	2 cut(s)	590, 602
EcoRII	CCWGG	1 cut(s)	777
EcoT14I	CCWWGG	1 cut(s)	519
EcoT38I	GRGCYC	1 cut(s)	597
ErhI	CCWWGG	1 cut(s)	519
FaeI	CATG	5 cut(s)	190, 412, 523, 847, 966
FaqI	GGGAC	4 cut(s)	304, 529, 542, 566
FatI	CATG	5 cut(s)	186, 408, 519, 843, 962
FauI	CCCGC	1 cut(s)	488
FbaI	TGATCA	1 cut(s)	484
Fnu4HI	GCNGC	2 cut(s)	333, 630
FokI	GGATG	2 cut(s)	171, 860
FriOI	GRGCYC	1 cut(s)	597
Fsp4HI	GCNGC	2 cut(s)	333, 630
FspBI	CTAG	1 cut(s)	536
GluI	GCNGC	2 cut(s)	333, 630
HaeIII	GGCC	3 cut(s)	59, 422, 777
HapII	CCGG	1 cut(s)	698
HgaI	GACGC	1 cut(s)	20
Hin1II	CATG	5 cut(s)	190, 412, 523, 847, 966
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HinfI	GANTC	2 cut(s)	909, 940
HpaI	GTTAAC	1 cut(s)	406
HpaII	CCGG	1 cut(s)	698
HphI	GGTGA	3 cut(s)	17, 353, 520
Hpy166II	GTNNAC	4 cut(s)	142, 344, 380, 406
Hpy188I	TCNGA	1 cut(s)	461
Hpy188III	TCNNGA	6 cut(s)	187, 286, 353, 439, 898, 963
Hpy8I	GTNNAC	4 cut(s)	142, 344, 380, 406
HpyAV	CCTTC	6 cut(s)	49, 129, 136, 493, 698, 796
HpyCH4III	ACNGT	3 cut(s)	152, 391, 416
HpyCH4IV	ACGT	1 cut(s)	831
HpyF10VI	GCNNNNNNNGC	1 cut(s)	638
HpyF3I	CTNAG	7 cut(s)	15, 30, 42, 264, 336, 458, 921
HpySE526I	ACGT	1 cut(s)	831
Hsp92II	CATG	5 cut(s)	190, 412, 523, 847, 966
Ksp22I	TGATCA	1 cut(s)	484
KspAI	GTTAAC	1 cut(s)	406
Kzo9I	GATC	1 cut(s)	484
LguI	GCTCTTC	1 cut(s)	99
LmnI	GCTCC	2 cut(s)	259, 731
Lsp1109I	GCAGC	2 cut(s)	319, 616
LweI	GCATC	1 cut(s)	861
MaeI	CTAG	1 cut(s)	536
MaeII	ACGT	1 cut(s)	831
MaeIII	GTNAC	5 cut(s)	238, 385, 478, 508, 827
MalI	GATC	1 cut(s)	486
MboI	GATC	1 cut(s)	484
MhlI	GDGCHC	1 cut(s)	597
MlsI	TGGCCA	1 cut(s)	777
MluCI	AATT	1 cut(s)	192
MluNI	TGGCCA	1 cut(s)	777
Mox20I	TGGCCA	1 cut(s)	777
MroXI	GAANNNNTTC	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	777
MseI	TTAA	1 cut(s)	405
Msp20I	TGGCCA	1 cut(s)	777
MspA1I	CMGCKG	3 cut(s)	332, 629, 925
MspI	CCGG	1 cut(s)	698
MspR9I	CCNGG	1 cut(s)	779
MvaI	CCWGG	1 cut(s)	779
MwoI	GCNNNNNNNGC	1 cut(s)	638
NcoI	CCATGG	1 cut(s)	519
NdeII	GATC	1 cut(s)	484
NlaIII	CATG	5 cut(s)	190, 412, 523, 847, 966
NlaIV	GGNNCC	2 cut(s)	261, 517
NmeAIII	GCCGAG	1 cut(s)	85
NmuCI	GTSAC	3 cut(s)	238, 478, 508
NspI	RCATGY	1 cut(s)	412
PagI	TCATGA	2 cut(s)	186, 962
PceI	AGGCCT	1 cut(s)	422
PciSI	GCTCTTC	1 cut(s)	99
PdmI	GAANNNNTTC	1 cut(s)	348
PfeI	GAWTC	2 cut(s)	909, 940
PkrI	GCNGC	2 cut(s)	334, 631
Ppu21I	YACGTR	1 cut(s)	832
Psp6I	CCWGG	1 cut(s)	777
PspGI	CCWGG	1 cut(s)	777
PspN4I	GGNNCC	2 cut(s)	261, 517
PspPI	GGNCC	2 cut(s)	180, 516
PstI	CTGCAG	1 cut(s)	634
PstNI	CAGNNNCTG	1 cut(s)	335
PvuII	CAGCTG	3 cut(s)	332, 629, 925
RsaI	GTAC	1 cut(s)	878
RsaNI	GTAC	1 cut(s)	877
SapI	GCTCTTC	1 cut(s)	99
SaqAI	TTAA	1 cut(s)	405
SatI	GCNGC	2 cut(s)	333, 630
Sau3AI	GATC	1 cut(s)	484
Sau96I	GGNCC	2 cut(s)	180, 516
ScaI	AGTACT	1 cut(s)	878
ScrFI	CCNGG	1 cut(s)	779
SduI	GDGCHC	1 cut(s)	597
SfaNI	GCATC	1 cut(s)	861
SfcI	CTRYAG	2 cut(s)	390, 630
SinI	GGWCC	2 cut(s)	180, 516
SmlI	CTYRAG	1 cut(s)	437
SmoI	CTYRAG	1 cut(s)	437
SnaBI	TACGTA	1 cut(s)	832
Sse9I	AATT	1 cut(s)	192
SseBI	AGGCCT	1 cut(s)	422
SsiI	CCGC	2 cut(s)	495, 729
SspMI	CTAG	1 cut(s)	536
StuI	AGGCCT	1 cut(s)	422
StyD4I	CCNGG	1 cut(s)	777
StyI	CCWWGG	1 cut(s)	519
TaaI	ACNGT	3 cut(s)	152, 391, 416
TaiI	ACGT	1 cut(s)	834
TaqI	TCGA	1 cut(s)	399
TasI	AATT	1 cut(s)	192
TatI	WGTACW	1 cut(s)	876
TfiI	GAWTC	2 cut(s)	909, 940
Tru1I	TTAA	1 cut(s)	405
Tru9I	TTAA	1 cut(s)	405
TscAI	CASTG	4 cut(s)	155, 276, 419, 937
TseFI	GTSAC	3 cut(s)	238, 478, 508
TseI	GCWGC	2 cut(s)	332, 629
Tsp45I	GTSAC	3 cut(s)	238, 478, 508
TspDTI	ATGAA	3 cut(s)	249, 875, 951
TspRI	CASTG	4 cut(s)	155, 276, 419, 937
VpaK11BI	GGWCC	2 cut(s)	180, 516
XceI	RCATGY	1 cut(s)	412
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	1 cut(s)	536
ZrmI	AGTACT	1 cut(s)	878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.