Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007146

Physical Location & Seq

Genomic Coordinates Reverse (-)

36028838 .. 36030828

Gene Structure 1991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1301700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGGTGAAGAGGCTAAGCAGGAGCAAGCTAAGGCAGAGGCTAAGGCAGAGGAGAAAGCCGAGGTGAAAGTAGAGGAAAAGAAAGACGAGAAGGCAGAGGAGGAAAAGAAGGAAGAGCCAAAACCTCCTTCTCCTTTTGTGTTGTTTGTAGACTTGCATTGTGTGGGATGTGCAAAGAAGATTGAGAGGTCCATCATGAGAATTAGAGGAGTGGAGGGGGTTGTGATAGACATGGCAAAAAATGAAGTGACCATAAAGGGAATAGTGGAGCCTCAGGCAGTTTGCAACAAAATCATGAAGAAAACCAAGAGAATAGCAAAAGTCTTGTCCCCATTACCAGCTGCTGAGGGTGAAGCTATTCCAGAAGTTGTTGCTTCACAGGTTAGTGGACTAGTAACTGTGGAACTCGAAGTTAACATGCACTGTGAGCCCTGCGCAGAGCAACTCAAGAGAAAGATTCTCAAACTCAGAGGAGTTCAAAGTGCTGTAACTGATCATAGTAGCGGGAAGGTGATGGTGACAGGGACCATGGACGGGGACAAGCTAGTAGAATATGTGTATAGACGAACCAAGAAGCAAGCCCGAATAGTCCCACAACCCGAGCCCGAACCCGAGAAGAAGGAGGAACCAGCTGAAGAAGAAGCAAAACCCGAAGAAAAGAAAGAAGAAAATGCAGAGAAAAAAGAAGAGGAAAAACCGGCAGAAGGGGAGGCAAAGAAAGAAGAGGGCGGAGCTGAAGGCGGAGAAAGTAGTAATAAGGAGGGGGAGAAAGGTGGTGAGGAGAACAAAGAAGAGGAGAAGGTGGAAGTGACGATGAGTGGCAGTTACGTAAATAGTGGAATGGATGAGGAACATATGAAAAGAATGATGCAGTACTATTACCAACCTCTTTACGTTATTGAGAGAATCCCACCTCCTCAGCTGTTCAGTGATGAGAATCCCAATGCATGTTGCATTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko04978 map04978

Pfam Domains

Protein Families

HMA

Protein Analysis

321

Amino Acids

35.73

Weight (kDa)

5.07

Isoelectric Point (pI)

59.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	48 - 100	4.7e-11	Heavy-metal-associated domain
HMA	PF00403	138 - 183	1.6e-07	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007146)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24580 AT5G24580 AT5G24580 AT5G24580 AT5G24580
fragaria_vesca	FvH4_7g33060
malus_domestica	MD01G1229700.v1.1 MD07G1301700.v1.1
prunus_persica	Prupe.2G320300_v2.0.a1
pyrus_communis	pycom01g23780 pycom07g27450
rosa_chinensis	RchiOBHm_Chr1g0330391 RchiOBHm_Chr1g0383071
rosa_laevigata	RLG00000026135
rosa_multiflora	Rmu_sc0000634.1_g000006 Rmu_sc0008563.1_g000016 Rmu_sc0033623.1_g000002
rosa_roxburghii	Rroxscaffold_4G00277640
rosa_rugosa	Rorug01G0438100
rosa_samantha	Rh1AG463800 Rh1BG421000 Rh1CG434500 Rh1DG453600
rosa_wichuraiana	Rw1G008460 Rw1G040840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	436
AccI	GTMKAC	1 cut(s)	150
AciI	CCGC	3 cut(s)	504, 729, 741
AcuI	CTGAAG	2 cut(s)	654, 756
AfaI	GTAC	1 cut(s)	875
AfiI	CCNNNNNNNGG	2 cut(s)	534, 704
AgsI	TTSAA	1 cut(s)	479
AhlI	ACTAGT	1 cut(s)	391
AjuI	GAANNNNNNNTTGG	2 cut(s)	112, 144
AluBI	AGCT	7 cut(s)	29, 341, 356, 544, 632, 734, 922
AluI	AGCT	7 cut(s)	29, 341, 356, 544, 632, 734, 922
AlwNI	CAGNNNCTG	1 cut(s)	344
Ama87I	CYCGRG	2 cut(s)	599, 611
ApeKI	GCWGC	1 cut(s)	341
ArsI	GACNNNNNNTTYG	2 cut(s)	311, 343
Asp700I	GAANNNNTTC	1 cut(s)	357
AspLEI	GCGC	1 cut(s)	437
AspS9I	GGNCC	2 cut(s)	189, 525
AsuHPI	GGTGA	6 cut(s)	17, 76, 362, 523, 529, 788
AvaI	CYCGRG	2 cut(s)	599, 611
AvaII	GGWCC	2 cut(s)	189, 525
AxyI	CCTNAGG	1 cut(s)	273
BanII	GRGCYC	2 cut(s)	432, 606
BbvCI	CCTCAGC	2 cut(s)	345, 918
BbvI	GCAGC	1 cut(s)	328
BccI	CCATC	2 cut(s)	200, 508
BclI	TGATCA	1 cut(s)	493
BcuI	ACTAGT	1 cut(s)	391
BfaI	CTAG	2 cut(s)	392, 545
BisI	GCNGC	1 cut(s)	342
BlpI	GCTNAGC	1 cut(s)	15
BlsI	GCNGC	1 cut(s)	343
BmcAI	AGTACT	1 cut(s)	875
Bme18I	GGWCC	2 cut(s)	189, 525
BmeT110I	CYCGRG	2 cut(s)	599, 611
BmgT120I	GGNCC	2 cut(s)	189, 525
BmiI	GGNNCC	3 cut(s)	270, 526, 627
BmsI	GCATC	1 cut(s)	858
Bpu10I	CCTNAGC	4 cut(s)	30, 42, 345, 918
Bpu1102I	GCTNAGC	1 cut(s)	15
BpuEI	CTTGAG	1 cut(s)	431
BsaAI	YACGTR	1 cut(s)	829
BsaBI	GATNNNNATC	1 cut(s)	936
BsaJI	CCNNGG	2 cut(s)	60, 528
Bsc4I	CCNNNNNNNGG	2 cut(s)	534, 704
Bse118I	RCCGGY	1 cut(s)	697
Bse21I	CCTNAGG	1 cut(s)	273
Bse8I	GATNNNNATC	1 cut(s)	936
BseDI	CCNNGG	2 cut(s)	60, 528
BseGI	GGATG	2 cut(s)	173, 850
BseJI	GATNNNNATC	1 cut(s)	936
BseLI	CCNNNNNNNGG	2 cut(s)	534, 704
BseMII	CTCAG	4 cut(s)	287, 336, 481, 932
BseRI	GAGGAG	7 cut(s)	65, 113, 222, 486, 794, 809, 906
BseXI	GCAGC	1 cut(s)	328
BsiHKCI	CYCGRG	2 cut(s)	599, 611
BsiSI	CCGG	1 cut(s)	698
BslFI	GGGAC	4 cut(s)	313, 538, 551, 575
BslI	CCNNNNNNNGG	2 cut(s)	534, 704
BsmFI	GGGAC	4 cut(s)	313, 538, 551, 575
BsoBI	CYCGRG	2 cut(s)	599, 611
Bsp1286I	GDGCHC	2 cut(s)	432, 606
Bsp143I	GATC	1 cut(s)	493
Bsp1720I	GCTNAGC	1 cut(s)	15
Bsp19I	CCATGG	1 cut(s)	528
BspACI	CCGC	3 cut(s)	504, 729, 741
BspCNI	CTCAG	4 cut(s)	286, 337, 480, 931
BspHI	TCATGA	3 cut(s)	195, 294, 959
BspLI	GGNNCC	3 cut(s)	270, 526, 627
BspQI	GCTCTTC	1 cut(s)	108
BsrFI	RCCGGY	1 cut(s)	697
BssAI	RCCGGY	1 cut(s)	697
BssECI	CCNNGG	2 cut(s)	60, 528
BssMI	GATC	1 cut(s)	493
BssT1I	CCWWGG	1 cut(s)	528
Bst4CI	ACNGT	2 cut(s)	400, 425
Bst6I	CTCTTC	5 cut(s)	3, 108, 681, 717, 786
BstBAI	YACGTR	1 cut(s)	829
BstC8I	GCNNGC	2 cut(s)	27, 579
BstDEI	CTNAG	7 cut(s)	15, 30, 42, 273, 345, 467, 918
BstDSI	CCRYGG	1 cut(s)	528
BstF5I	GGATG	2 cut(s)	173, 850
BstHHI	GCGC	1 cut(s)	437
BstKTI	GATC	1 cut(s)	496
BstMBI	GATC	1 cut(s)	493
BstMWI	GCNNNNNNNGC	1 cut(s)	427
BstNSI	RCATGY	2 cut(s)	421, 951
BstSNI	TACGTA	1 cut(s)	829
BstV1I	GCAGC	1 cut(s)	328
Bsu36I	CCTNAGG	1 cut(s)	273
BtgI	CCRYGG	1 cut(s)	528
BtsCI	GGATG	2 cut(s)	173, 850
BtsIMutI	CAGTG	2 cut(s)	421, 934
Cac8I	GCNNGC	2 cut(s)	27, 579
CaiI	CAGNNNCTG	1 cut(s)	344
CciI	TCATGA	3 cut(s)	195, 294, 959
CfoI	GCGC	1 cut(s)	437
Cfr10I	RCCGGY	1 cut(s)	697
Cfr13I	GGNCC	2 cut(s)	189, 525
Csp6I	GTAC	1 cut(s)	874
CviAII	CATG	7 cut(s)	196, 232, 295, 418, 529, 948, 960
CviQI	GTAC	1 cut(s)	874
DdeI	CTNAG	7 cut(s)	15, 30, 42, 273, 345, 467, 918
DpnI	GATC	1 cut(s)	495
DpnII	GATC	1 cut(s)	493
Eam1104I	CTCTTC	5 cut(s)	3, 108, 681, 717, 786
EarI	CTCTTC	5 cut(s)	3, 108, 681, 717, 786
EciI	GGCGGA	2 cut(s)	744, 756
Eco105I	TACGTA	1 cut(s)	829
Eco130I	CCWWGG	1 cut(s)	528
Eco24I	GRGCYC	2 cut(s)	432, 606
Eco47I	GGWCC	2 cut(s)	189, 525
Eco57I	CTGAAG	2 cut(s)	654, 756
Eco81I	CCTNAGG	1 cut(s)	273
Eco88I	CYCGRG	2 cut(s)	599, 611
EcoT14I	CCWWGG	1 cut(s)	528
EcoT22I	ATGCAT	1 cut(s)	949
EcoT38I	GRGCYC	2 cut(s)	432, 606
ErhI	CCWWGG	1 cut(s)	528
FaeI	CATG	7 cut(s)	199, 235, 298, 421, 532, 951, 963
FaqI	GGGAC	4 cut(s)	313, 538, 551, 575
FatI	CATG	7 cut(s)	195, 231, 294, 417, 528, 947, 959
FauI	CCCGC	1 cut(s)	497
FauNDI	CATATG	1 cut(s)	855
FbaI	TGATCA	1 cut(s)	493
FblI	GTMKAC	1 cut(s)	150
Fnu4HI	GCNGC	1 cut(s)	342
FokI	GGATG	2 cut(s)	180, 857
FriOI	GRGCYC	2 cut(s)	432, 606
Fsp4HI	GCNGC	1 cut(s)	342
FspBI	CTAG	2 cut(s)	392, 545
FspI	TGCGCA	1 cut(s)	436
GlaI	GCGC	1 cut(s)	436
GluI	GCNGC	1 cut(s)	342
HapII	CCGG	1 cut(s)	698
HhaI	GCGC	1 cut(s)	437
Hin1II	CATG	7 cut(s)	199, 235, 298, 421, 532, 951, 963
Hin6I	GCGC	1 cut(s)	435
HinP1I	GCGC	1 cut(s)	435
HincII	GTYRAC	1 cut(s)	415
HindII	GTYRAC	1 cut(s)	415
HinfI	GANTC	3 cut(s)	457, 906, 937
HpaI	GTTAAC	1 cut(s)	415
HpaII	CCGG	1 cut(s)	698
HphI	GGTGA	6 cut(s)	17, 76, 362, 523, 529, 788
Hpy166II	GTNNAC	3 cut(s)	151, 389, 415
Hpy188I	TCNGA	1 cut(s)	470
Hpy188III	TCNNGA	5 cut(s)	196, 295, 362, 448, 960
Hpy8I	GTNNAC	3 cut(s)	151, 389, 415
HpyAV	CCTTC	8 cut(s)	85, 103, 138, 502, 613, 698, 731, 793
HpyCH4III	ACNGT	2 cut(s)	400, 425
HpyCH4IV	ACGT	2 cut(s)	828, 894
HpyCH4V	TGCA	8 cut(s)	157, 173, 285, 421, 674, 871, 947, 954
HpyF10VI	GCNNNNNNNGC	1 cut(s)	427
HpyF3I	CTNAG	7 cut(s)	15, 30, 42, 273, 345, 467, 918
HpySE526I	ACGT	2 cut(s)	828, 894
Hsp92II	CATG	7 cut(s)	199, 235, 298, 421, 532, 951, 963
HspAI	GCGC	1 cut(s)	435
Ksp22I	TGATCA	1 cut(s)	493
KspAI	GTTAAC	1 cut(s)	415
Kzo9I	GATC	1 cut(s)	493
LguI	GCTCTTC	1 cut(s)	108
LmnI	GCTCC	3 cut(s)	22, 268, 731
LpnPI	CCDG	9 cut(s)	5, 260, 351, 365, 375, 445, 507, 642, 711
Lsp1109I	GCAGC	1 cut(s)	328
LweI	GCATC	1 cut(s)	858
MaeI	CTAG	2 cut(s)	392, 545
MaeII	ACGT	2 cut(s)	828, 894
MaeIII	GTNAC	6 cut(s)	247, 394, 487, 517, 808, 824
MalI	GATC	1 cut(s)	495
MboI	GATC	1 cut(s)	493
MhlI	GDGCHC	2 cut(s)	432, 606
MluCI	AATT	1 cut(s)	201
Mph1103I	ATGCAT	1 cut(s)	949
MroXI	GAANNNNTTC	1 cut(s)	357
MseI	TTAA	1 cut(s)	414
MspA1I	CMGCKG	3 cut(s)	341, 632, 922
MspI	CCGG	1 cut(s)	698
MwoI	GCNNNNNNNGC	1 cut(s)	427
NcoI	CCATGG	1 cut(s)	528
NdeI	CATATG	1 cut(s)	855
NdeII	GATC	1 cut(s)	493
NlaIII	CATG	7 cut(s)	199, 235, 298, 421, 532, 951, 963
NlaIV	GGNNCC	3 cut(s)	270, 526, 627
NmeAIII	GCCGAG	1 cut(s)	85
NmuCI	GTSAC	3 cut(s)	247, 517, 808
NsbI	TGCGCA	1 cut(s)	436
NsiI	ATGCAT	1 cut(s)	949
NspI	RCATGY	2 cut(s)	421, 951
PagI	TCATGA	3 cut(s)	195, 294, 959
PciSI	GCTCTTC	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	357
PfeI	GAWTC	3 cut(s)	457, 906, 937
PkrI	GCNGC	1 cut(s)	343
Ppu21I	YACGTR	1 cut(s)	829
PspN4I	GGNNCC	3 cut(s)	270, 526, 627
PspPI	GGNCC	2 cut(s)	189, 525
PstNI	CAGNNNCTG	1 cut(s)	344
PvuII	CAGCTG	3 cut(s)	341, 632, 922
RsaI	GTAC	1 cut(s)	875
RsaNI	GTAC	1 cut(s)	874
SapI	GCTCTTC	1 cut(s)	108
SaqAI	TTAA	1 cut(s)	414
SatI	GCNGC	1 cut(s)	342
Sau3AI	GATC	1 cut(s)	493
Sau96I	GGNCC	2 cut(s)	189, 525
ScaI	AGTACT	1 cut(s)	875
SduI	GDGCHC	2 cut(s)	432, 606
SfaNI	GCATC	1 cut(s)	858
SinI	GGWCC	2 cut(s)	189, 525
SmlI	CTYRAG	1 cut(s)	446
SmoI	CTYRAG	1 cut(s)	446
SnaBI	TACGTA	1 cut(s)	829
SpeI	ACTAGT	1 cut(s)	391
Sse9I	AATT	1 cut(s)	201
SsiI	CCGC	3 cut(s)	504, 729, 741
SspMI	CTAG	2 cut(s)	392, 545
StyI	CCWWGG	1 cut(s)	528
TaaI	ACNGT	2 cut(s)	400, 425
TaiI	ACGT	2 cut(s)	831, 897
TaqI	TCGA	1 cut(s)	408
TasI	AATT	1 cut(s)	201
TatI	WGTACW	1 cut(s)	873
TfiI	GAWTC	3 cut(s)	457, 906, 937
Tru1I	TTAA	1 cut(s)	414
Tru9I	TTAA	1 cut(s)	414
TscAI	CASTG	2 cut(s)	428, 934
TseFI	GTSAC	3 cut(s)	247, 517, 808
TseI	GCWGC	1 cut(s)	341
Tsp45I	GTSAC	3 cut(s)	247, 517, 808
TspDTI	ATGAA	4 cut(s)	258, 311, 872, 948
TspRI	CASTG	2 cut(s)	428, 934
VpaK11BI	GGWCC	2 cut(s)	189, 525
XceI	RCATGY	2 cut(s)	421, 951
XmiI	GTMKAC	1 cut(s)	150
XmnI	GAANNNNTTC	1 cut(s)	357
XspI	CTAG	2 cut(s)	392, 545
ZrmI	AGTACT	1 cut(s)	875
Zsp2I	ATGCAT	1 cut(s)	949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.