Rw1G008460

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007146
Physical Location & Seq
Forward (+)
18299256 .. 18300595
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G008460.1

Sequence Viewer

Length: 831 bp
ATGGGTGAAGAAACTAATCCGGAAGAAATTAAGGTAGAGGAGGTTAAGCCAGAAGAGAAGGCAGAAGAGAAGAAAGAAGAGAAGGCAGAGGAGGAGAAGAAGGAAGAGCCAAAACCACCAGCTCCTTTTGTCCTATTTGTGGACTTGCATTGTGTGGGGTGTGCAAAGAAAATTGAGAGGTCTATCATGAAAATTAGAGTGACCATAAAGGGAATAGTGGAGCCTCAAGCAGTGTGCAACAAAACCATGAAAAGAACCAAGAGAAAGGCTCAAGTCTTGTCCCCATTACCAGCTGCTGAGGGTGAACCTATTCCAGAAATTGTTGCTTCTCAGGTCAGTGGAGTAACAACTGTGGAACTCCAAGTTAACATGCACTGTGAGGCCTGTGCAGAACAACTTAAGAAAAAGATACTCAAAATGAGAGGGGTTCAAAATGCCGTGACTGATCATACTAGCGGGAAGGTGACCGTGACGGGGACAATGGGCAGCGACAAACTAGTAGACTACGTTTACAGACGATCGAACCAGAAAACAAGCCAAAATCCCGAAGAAGCAAAGCCGGAAGAGAATGCGGAGAAGAAAGAGGAGGAAAAGCCCGCAGAAGAGGCAACGAAAGAAGAAGGTGGCGGTGGAGAAAGTAATAAAGAGGGTGAGGAAAAGAAAGAGGAGGAGACCAAGATTGAAGAGCCTAACGACGTCGTTAATGGTATACCGGAGGAGGAATTGAAGAGGATGGTGCAGCACTATTATCAGCCGCTTTACGTGTTTGAGAGAATCCCGCCGCCGCAGCTCTTCAGCGATGAGAATCCAAATGCATGTTGCATTTCGTAA

Protein Analysis

276

Amino Acids

31.0

Weight (kDa)

5.07

Isoelectric Point (pI)

64.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 121 - 164 2.4e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 699
AccI GTMKAC 2 cut(s) 501, 709
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 8 cut(s) 456, 572, 597, 627, 755, 779, 782, 785
AcuI CTGAAG 1 cut(s) 778
AcyI GRCGYC 1 cut(s) 696
AfiI CCNNNNNNNGG 2 cut(s) 139, 474
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 3 cut(s) 431, 683, 727
AhlI ACTAGT 1 cut(s) 496
AluBI AGCT 3 cut(s) 122, 293, 790
AluI AGCT 3 cut(s) 122, 293, 790
Alw26I GTCTC 1 cut(s) 665
AlwNI CAGNNNCTG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 4 cut(s) 293, 486, 739, 787
Asp700I GAANNNNTTC 1 cut(s) 309
AsuHPI GGTGA 4 cut(s) 17, 314, 475, 662
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 4 cut(s) 280, 498, 751, 799
BccI CCATC 1 cut(s) 727
BceAI ACGGC 1 cut(s) 422
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 665
BcuI ACTAGT 1 cut(s) 496
BfaI CTAG 2 cut(s) 453, 497
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 7 cut(s) 294, 487, 740, 755, 782, 785, 788
BlsI GCNGC 7 cut(s) 295, 488, 741, 756, 783, 786, 789
BmiI GGNNCC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 253, 285
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 210, 255
BsaAI YACGTR 1 cut(s) 763
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 696
BsaI GGTCTC 1 cut(s) 665
BsaWI WCCGGW 2 cut(s) 19, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 474
Bse8I GATNNNNATC 1 cut(s) 804
BseAI TCCGGA 1 cut(s) 19
BseGI GGATG 1 cut(s) 738
BseJI GATNNNNATC 1 cut(s) 804
BseLI CCNNNNNNNGG 2 cut(s) 139, 474
BseMII CTCAG 2 cut(s) 288, 344
BseRI GAGGAG 7 cut(s) 53, 104, 107, 599, 680, 683, 731
BseXI GCAGC 4 cut(s) 280, 498, 751, 799
BsgI GTGCAG 2 cut(s) 408, 758
Bsh1285I CGRYCG 1 cut(s) 521
BshFI GGCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 521
BsiSI CCGG 3 cut(s) 20, 560, 713
BslFI GGGAC 2 cut(s) 265, 490
BslI CCNNNNNNNGG 2 cut(s) 139, 474
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 2 cut(s) 265, 490
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 665
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 2 cut(s) 445, 518
BspACI CCGC 8 cut(s) 456, 572, 597, 627, 755, 779, 782, 785
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 289, 343
BspEI TCCGGA 1 cut(s) 19
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 222
BspQI GCTCTTC 3 cut(s) 99, 678, 797
BspTI CTTAAG 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 665
BssMI GATC 2 cut(s) 445, 518
BssNAI GTATAC 1 cut(s) 710
BssNI GRCGYC 1 cut(s) 696
Bst1107I GTATAC 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 352, 377, 469
Bst6I CTCTTC 9 cut(s) 48, 60, 72, 99, 558, 597, 678, 722, 797
BstACI GRCGYC 1 cut(s) 696
BstAFI CTTAAG 1 cut(s) 398
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 2 cut(s) 297, 330
BstEII GGTNACC 1 cut(s) 463
BstF5I GGATG 1 cut(s) 738
BstKTI GATC 2 cut(s) 448, 521
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 2 cut(s) 445, 518
BstMCI CGRYCG 1 cut(s) 521
BstMWI GCNNNNNNNGC 2 cut(s) 605, 787
BstNSI RCATGY 2 cut(s) 373, 819
BstPI GGTNACC 1 cut(s) 463
BstV1I GCAGC 4 cut(s) 280, 498, 751, 799
BstZ17I GTATAC 1 cut(s) 710
BsuRI GGCC 1 cut(s) 383
BtgZI GCGATG 1 cut(s) 813
BtsCI GGATG 1 cut(s) 738
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 3 cut(s) 237, 343, 373
Cac8I GCNNGC 1 cut(s) 597
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 186
CviAII CATG 4 cut(s) 187, 247, 370, 816
DdeI CTNAG 2 cut(s) 297, 330
DpnI GATC 2 cut(s) 447, 520
DpnII GATC 2 cut(s) 445, 518
Eam1104I CTCTTC 9 cut(s) 48, 60, 72, 99, 558, 597, 678, 722, 797
EarI CTCTTC 9 cut(s) 48, 60, 72, 99, 558, 597, 678, 722, 797
Eco147I AGGCCT 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 778
Eco91I GGTNACC 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 817
FaeI CATG 4 cut(s) 190, 250, 373, 819
FaiI YATR 7 cut(s) 188, 206, 248, 371, 450, 710, 817
FaqI GGGAC 2 cut(s) 265, 490
FatI CATG 4 cut(s) 186, 246, 369, 815
FauI CCCGC 3 cut(s) 449, 604, 786
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 2 cut(s) 501, 709
Fnu4HI GCNGC 7 cut(s) 294, 487, 740, 755, 782, 785, 788
FokI GGATG 1 cut(s) 745
Fsp4HI GCNGC 7 cut(s) 294, 487, 740, 755, 782, 785, 788
FspBI CTAG 2 cut(s) 453, 497
GluI GCNGC 7 cut(s) 294, 487, 740, 755, 782, 785, 788
HaeIII GGCC 1 cut(s) 383
HapII CCGG 3 cut(s) 20, 560, 713
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 4 cut(s) 190, 250, 373, 819
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 2 cut(s) 774, 805
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 3 cut(s) 20, 560, 713
HphI GGTGA 4 cut(s) 17, 314, 475, 662
Hpy166II GTNNAC 6 cut(s) 142, 305, 367, 502, 511, 710
Hpy188III TCNNGA 4 cut(s) 20, 187, 314, 545
Hpy8I GTNNAC 6 cut(s) 142, 305, 367, 502, 511, 710
Hpy99I CGWCG 2 cut(s) 698, 701
HpyAV CCTTC 5 cut(s) 52, 76, 94, 454, 614
HpyCH4III ACNGT 3 cut(s) 352, 377, 469
HpyCH4IV ACGT 3 cut(s) 507, 696, 762
HpyCH4V TGCA 8 cut(s) 148, 164, 237, 373, 389, 739, 815, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 787
HpyF3I CTNAG 2 cut(s) 297, 330
HpySE526I ACGT 3 cut(s) 507, 696, 762
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 4 cut(s) 190, 250, 373, 819
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 2 cut(s) 445, 518
LguI GCTCTTC 3 cut(s) 99, 678, 797
LmnI GCTCC 2 cut(s) 127, 220
Lsp1109I GCAGC 4 cut(s) 280, 498, 751, 799
MaeI CTAG 2 cut(s) 453, 497
MaeII ACGT 3 cut(s) 507, 696, 762
MaeIII GTNAC 5 cut(s) 199, 343, 439, 463, 469
MalI GATC 2 cut(s) 447, 520
MboI GATC 2 cut(s) 445, 518
MluCI AATT 5 cut(s) 27, 171, 192, 318, 722
Mph1103I ATGCAT 1 cut(s) 817
MroI TCCGGA 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 5 cut(s) 30, 45, 366, 399, 702
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 3 cut(s) 20, 560, 713
Mva1269I GAATGC 1 cut(s) 574
MwoI GCNNNNNNNGC 2 cut(s) 605, 787
NdeII GATC 2 cut(s) 445, 518
NlaIII CATG 4 cut(s) 190, 250, 373, 819
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 4 cut(s) 199, 439, 463, 469
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 2 cut(s) 373, 819
PagI TCATGA 1 cut(s) 186
PceI AGGCCT 1 cut(s) 383
PciSI GCTCTTC 3 cut(s) 99, 678, 797
PctI GAATGC 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 2 cut(s) 774, 805
PkrI GCNGC 7 cut(s) 295, 488, 741, 756, 783, 786, 789
Ple19I CGATCG 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 763
PspEI GGTNACC 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 296
PvuI CGATCG 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 293
SapI GCTCTTC 3 cut(s) 99, 678, 797
SaqAI TTAA 5 cut(s) 30, 45, 366, 399, 702
SatI GCNGC 7 cut(s) 294, 487, 740, 755, 782, 785, 788
Sau3AI GATC 2 cut(s) 445, 518
SmlI CTYRAG 3 cut(s) 225, 270, 398
SmoI CTYRAG 3 cut(s) 225, 270, 398
SpeI ACTAGT 1 cut(s) 496
Sse9I AATT 5 cut(s) 27, 171, 192, 318, 722
SseBI AGGCCT 1 cut(s) 383
SsiI CCGC 8 cut(s) 456, 572, 597, 627, 755, 779, 782, 785
SspMI CTAG 2 cut(s) 453, 497
StuI AGGCCT 1 cut(s) 383
TaaI ACNGT 3 cut(s) 352, 377, 469
TaiI ACGT 3 cut(s) 510, 699, 765
TaqI TCGA 1 cut(s) 521
TasI AATT 5 cut(s) 27, 171, 192, 318, 722
TauI GCSGC 3 cut(s) 757, 784, 787
TfiI GAWTC 2 cut(s) 774, 805
Tru1I TTAA 5 cut(s) 30, 45, 366, 399, 702
Tru9I TTAA 5 cut(s) 30, 45, 366, 399, 702
TscAI CASTG 3 cut(s) 237, 343, 380
TseFI GTSAC 4 cut(s) 199, 439, 463, 469
TseI GCWGC 4 cut(s) 293, 486, 739, 787
Tsp45I GTSAC 4 cut(s) 199, 439, 463, 469
TspDTI ATGAA 2 cut(s) 203, 263
TspRI CASTG 3 cut(s) 237, 343, 380
Vha464I CTTAAG 1 cut(s) 398
XceI RCATGY 2 cut(s) 373, 819
XmiI GTMKAC 2 cut(s) 501, 709
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 2 cut(s) 453, 497
ZraI GACGTC 1 cut(s) 697
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.