Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010104

Physical Location & Seq

Genomic Coordinates Forward (+)

32380665 .. 32394974

Gene Structure 14310 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1236400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

ATGGCGTCTCTACCTTTGAAGAAGAACTACCGGTGCGTGCCCTCTCTGCAACAGTTCTACTCCGGCGGCCCCTTCGCCGTCTCATCCGACGGCTCCTCCATCGCCTGCAAATGCGGCGAGTCCATTAAGATCGTGGATTCATTGGACGCCTCGATTCGGTCCACTATTGAAGGCGACTTAGACGAAGTTACCGCTTTGGCCCTCAGCCCCGACGACACTCTGCTCTTCTCCGCCGGTCACAGTCGCCAAATTAGGATTTGGGACCTCTCCACCCTCAAGTGCGTGCGTTCTTGGAAGGGTCATGAGGGGCCAGTGATGGGAATGGCTTGTCACCCGTCTGGAGGATTGCTTGCGACTGCCGGAGCTGATAGGAAGGTTCTTGTTTGGGATGTTGATGGCAGCTTTTGCACTCATTACTTTAAAGGCCACAAAGGGGTTGTTTCTAGTGTTCTCTTCCATCCTGATCCTACTAAACAACTTCTTTTCTCCGCAAGCGATGATACAACTGTGCTTGTTTGGGACTTGTTAACCAAGAAGTGCGTTGCAACGCTTAGTGGGCATAACTCCACAGTAACTTCTATGGCACTCTCTGAAGACGGATGGACATTGCTCACAGCTGGAAGAGATAAGGTTGTAATTTTGTGGGACCTCCATGACTATAGCTGCATGAAGACTGTAACAACATATGAGGTTCTTGAAGCTGTGTGCGCAATCTATTCTGGAACTCCATTGTCATCCTATTTGGGTTCCTGCAAGAAGCAGAATGGGAAGAAAAGTGGATTAAGTGAAACTTGTTTTATAACAGTTGGTGACCGTGGGATTGTAAGGATTTGGAATTCTGAAGGTTCAGTTTGCCTATATGAGCAAAAGTCCTCAGATGTCACTCCATCTTCAGATGTGGATGAATCAAATAGGGGATTCACCGCAGCCGTCATGCTTCCTTCAGATAAAGGGTTGCTTTGTGTGACTGCTGATCAGGAGTTCCTTTTATATTCCCCGGTTAAGTTGCCAGAGGGGACGTTGGAATTTGAATTGAGCAAAAGACTTGTAGGTTACAACGATGAGATTGTGGATATGAAGTTTGTAGGAGATGACGAACAATTCCTTGCTGTTGCCACAAATCTTGAACAGGTACGAGTATATGACATTGCGTCTATGTCATGTTCTTATGTATTGGCAGGTCATACTGGAATTGTTCTATGCCTTGACACCTGTGTATTGAGTTGTGGAAAACCACTTATTGTGACTGGGAGCAAAGACAACACTGTTCGGTTGTGGGATTCAGAAAGCAAATGTTGCCTTGGTCTTGGCATAGGTCACATGGGAGGTATTGGAGCTATTGCTTTCTCAAAAAAGCGGAAAGACTTCTTCGTTAGTGGCAGTAGTGATCGTACCATCAAGGTGTGGAGTTTGGATGGTCTTTCAGACAATACAGAAAAGCCAGTGAATCTGAAAGCAAAGGCTGGTGTAGCAGCCCATGATAGGGATATCAATTCAGTAGCTGTTGCACCAAATGATAGTTTAGTTTGTAGTGGTTCACAGGATCGCACTGCTTGTGTATGGAGGCTTCCGGATCTTGTACCATTAATTGTACTTAAGGGTCATAAAAGGGGGGTTTGGTCTGTAGAGTTCTCTCCCGTTGATCAATGTGTTATAACAGCTTCTGGTGATAAAACAATAAAGATATGGGCCATATCTGATGGTTTGTGCTTGAAAACATTTGAAGGGCATACATCAAGCGTATTAAGAGCATCGTTTCTTACTCGTGGAACCCAATTTATTTCTTCAGGTGCTGACGGGTTGGTAAAACTGTGGATGGTCAAAACCGATGAATGCATTGCTACTTATGATCAGCATGACAACAAGGTTTATGCCTTGGCTGTTGGAAAAAAGGCAGAAATGCTTGCAACTGGCAGCAGCGATGCTGTCATCAATATGTGGTATGATTGTACTGCTTCTGATAAAGAGGAAGCTTTTCGTAGAGAGGAGGAAGGTGTTTTAAAGAATCAAGAGCTAGAAAATGCTGTGTTAGATGCCGACTTTTCTAAAGCAATCCAAGTTGCATTTGAGCTTCGCAGGCCTCATAAGCTTTATAAGTGCTTTTCTGAAATTTGGAGGAAGAGAGAGACTGAAAAGCAGATGGAGAAAGCCCTTCTTGCCCTTGGCGAGGAAGAGATCAAGCTACTATTTGAATATGTTCGGGAATGGAATACAAAGCCAAAGCTCTGTCATGTTGCTCACTTTGTGCTTTCAAAGGTTTTCAACATCCTCAGTCCAACAAAGATTAATGAGATAAAAGGCATTGGGGAAATCCTTGAAGGTCTCCTCTCCTATTCTCAGAGGCATTTTAGCCGAATGGACAGGCACGAAACGAACACGTATTTGGTGAACTACACTCTGACTGGAATGTCAGTCATCGAACCAGAGACAGATACACGAGTATTGCATGACAAGTCTTTGTTGCACTCCTCTGCGGACGATGAGAAAGGGACTTTAATAAACGAACCTGAAGATGAGGAGCGAAAAACCTCTCAACGTTTTAAAGAAGAGGCAGCTACGAAGAAACGGAAGTCAAAGAAATCAAAAGATGGTTCCACTTGCAATAAGAAAGTTAAGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp13 WD40

Protein Analysis

874

Amino Acids

96.12

Weight (kDa)

6.14

Isoelectric Point (pI)

35.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	25 - 155	2.6e-19	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	26 - 100	4.7e-07	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	27 - 156	4.7e-22	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	33 - 182	2.8e-16	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	37 - 243	2e-18	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	41 - 236	6.6e-36	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	57 - 235	6e-18	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	62 - 216	2.8e-22	WDR3 second beta-propeller domain
Beta-prop_DCAF4	PF23761	62 - 131	5.1e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	63 - 215	6.1e-17	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	63 - 249	2.1e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	64 - 202	2.1e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	66 - 175	4.3e-11	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	83 - 215	5.5e-12	WDR36/Utp21 first beta-propeller
WD40	PF00400	93 - 130	1.3e-08	WD domain, G-beta repeat
WDR55	PF24796	95 - 224	1.1e-10	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	99 - 225	1.3e-14	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	103 - 184	9.6e-10	WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	112 - 222	1.1e-09	WDR90/POC16, second beta-propeller
Beta-prop_SCAP	PF24017	113 - 216	1.6e-07	SCAP Beta-propeller
Beta-prop_Aladin	PF25460	122 - 236	2.6e-07	Aladin seven-bladed propeller
Beta-prop_WDR3_1st	PF25173	128 - 228	4.7e-22	WDR3 first beta-propeller domain
WD40	PF00400	136 - 174	1.1e-06	WD domain, G-beta repeat
EIF3I	PF24805	140 - 253	2.9e-08	EIF3I
Beta-prop_THOC3	PF25174	156 - 230	2.2e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	157 - 233	4.9e-09	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	158 - 235	1.2e-15	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	158 - 227	7.4e-08	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	161 - 217	6.4e-06	MABP1/WDR62 second WD40 domain
WD40	PF00400	179 - 216	2.4e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	264 - 522	2.3e-19	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	265 - 433	5.2e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	312 - 471	2.9e-19	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	314 - 471	2e-08	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	350 - 472	3.5e-15	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	352 - 472	5.8e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	353 - 475	2e-10	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	362 - 606	1.1e-18	MABP1/WDR62 second WD40 domain
WD40	PF00400	387 - 427	4.3e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	388 - 524	1.2e-10	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	400 - 473	3.8e-08	Prp19 WD40 domain
Beta-prop_SCAP	PF24017	413 - 470	9.4e-06	SCAP Beta-propeller
Beta-prop_TEP1_2nd	PF25047	413 - 608	1e-16	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	439 - 616	5.7e-18	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	440 - 648	2.6e-31	WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	446 - 572	4.4e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	452 - 589	8.6e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	453 - 572	2.4e-06	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	485 - 647	9.7e-20	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	487 - 647	2e-24	Prp19 WD40 domain
EIF3I	PF24805	491 - 587	9.3e-12	EIF3I
Beta-prop_WDR3_1st	PF25173	491 - 647	3.4e-32	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	491 - 649	5.4e-36	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	492 - 597	1e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	492 - 650	4.8e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	493 - 577	5.8e-14	THOC3 beta-propeller domain
WDR55	PF24796	494 - 647	3.5e-11	WDR55
WD40	PF00400	528 - 563	1.9e-08	WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd	PF24782	536 - 647	4.8e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	548 - 677	2.3e-07	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	548 - 652	4.6e-14	G protein beta WD-40 repeat protein
Beta-prop_SCAP	PF24017	551 - 651	1.8e-06	SCAP Beta-propeller
WD40	PF00400	569 - 605	4.6e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	570 - 648	4.8e-12	THOC3 beta-propeller domain
EIF3I	PF24805	574 - 648	3.2e-07	EIF3I
Utp13	PF08625	669 - 803	4.9e-39	Utp13 specific WD40 associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16750
fragaria_vesca	FvH4_7g34000 FvH4_7g34000
malus_domestica	MD01G1236400.v1.1 MD07G1309100.v1.1
prunus_persica	Prupe.2G326400_v2.0.a1 Prupe.2G326400_v2.0.a1
pyrus_communis	pycom01g24430 pycom07g28110
rosa_chinensis	RchiOBHm_Chr1g0384251
rosa_laevigata	RLG00000026054
rosa_multiflora	Rmu_sc0011271.1_g000002
rosa_roxburghii	Rroxscaffold_4G00276810
rosa_rugosa	Rorug01G0446100 Rorug01G0446200 Rorug01G0446200
rosa_samantha	Rh1AG471700 Rh1BG429400 Rh1CG442000 Rh1DG462100
rosa_wichuraiana	Rw1G040090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	800, 1655, 2094
AasI	GACNNNNNNGTC	1 cut(s)	2407
Acc16I	TGCGCA	1 cut(s)	709
Acc36I	ACCTGC	1 cut(s)	1169
AccIII	TCCGGA	1 cut(s)	1570
AciI	CCGC	8 cut(s)	66, 114, 192, 231, 489, 924, 1357, 2474
AclI	AACGTT	1 cut(s)	2536
AclWI	GGATC	3 cut(s)	458, 1551, 1581
AcsI	RAATTY	3 cut(s)	835, 1025, 2109
AcuI	CTGAAG	6 cut(s)	612, 861, 876, 927, 1770, 2529
AcyI	GRCGYC	2 cut(s)	5, 147
AdeI	CACNNNGTG	1 cut(s)	2245
AfaI	GTAC	5 cut(s)	1134, 1393, 1581, 1593, 1951
AfiI	CCNNNNNNNGG	7 cut(s)	156, 317, 341, 433, 1482, 1483, 2167
AflII	CTTAAG	1 cut(s)	1595
AflIII	ACRYGT	1 cut(s)	2376
AgeI	ACCGGT	1 cut(s)	30
AhdI	GACNNNNNGTC	1 cut(s)	1150
AjuI	GAANNNNNNNTTGG	2 cut(s)	2366, 2398
Alw26I	GTCTC	5 cut(s)	12, 85, 2120, 2327, 2420
AlwI	GGATC	3 cut(s)	458, 1551, 1581
AlwNI	CAGNNNCTG	3 cut(s)	1502, 1664, 1793
Aor13HI	TCCGGA	1 cut(s)	1570
AoxI	GGCC	6 cut(s)	67, 198, 308, 424, 1689, 2078
ApeKI	GCWGC	7 cut(s)	399, 663, 926, 1472, 1914, 1917, 2552
ApoI	RAATTY	3 cut(s)	835, 1025, 2109
AseI	ATTAAT	2 cut(s)	1586, 2286
AsiGI	ACCGGT	1 cut(s)	30
Asp700I	GAANNNNTTC	3 cut(s)	1364, 1974, 2196
AspLEI	GCGC	1 cut(s)	710
AspS9I	GGNCC	7 cut(s)	68, 159, 199, 262, 308, 646, 1689
AsuC2I	CCSGG	1 cut(s)	998
AsuHPI	GGTGA	5 cut(s)	323, 821, 913, 1679, 2398
AvaII	GGWCC	3 cut(s)	159, 262, 646
BaeGI	GKGCMC	1 cut(s)	42
BaeI	ACNNNNGTAYC	4 cut(s)	492, 525, 2424, 2457
BarI	GAAGNNNNNNTAC	4 cut(s)	11, 43, 1551, 1583
BauI	CACGAG	2 cut(s)	1764, 2436
BbsI	GAAGAC	2 cut(s)	600, 677
BbvCI	CCTCAGC	1 cut(s)	203
BbvI	GCAGC	7 cut(s)	411, 650, 938, 1484, 1926, 1929, 2564
BceAI	ACGGC	3 cut(s)	62, 106, 914
BclI	TGATCA	3 cut(s)	973, 1642, 1849
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	5 cut(s)	12, 85, 2120, 2327, 2420
BfaI	CTAG	2 cut(s)	444, 2015
BfmI	CTRYAG	2 cut(s)	658, 1623
BfrI	CTTAAG	1 cut(s)	1595
BfuAI	ACCTGC	1 cut(s)	1169
BisI	GCNGC	9 cut(s)	67, 115, 400, 664, 927, 1473, 1915, 1918, 2553
BlsI	GCNGC	9 cut(s)	68, 116, 401, 665, 928, 1474, 1916, 1919, 2554
Bme1390I	CCNGG	1 cut(s)	998
Bme18I	GGWCC	3 cut(s)	159, 262, 646
BmeRI	GACNNNNNGTC	1 cut(s)	1150
BmgT120I	GGNCC	7 cut(s)	68, 159, 199, 262, 308, 646, 1689
BmiI	GGNNCC	8 cut(s)	70, 94, 263, 309, 647, 748, 1771, 2593
BmrFI	CCNGG	1 cut(s)	998
BmrI	ACTGGG	1 cut(s)	1257
BmsI	GCATC	3 cut(s)	1760, 1912, 2023
BmuI	ACTGGG	1 cut(s)	1257
BpiI	GAAGAC	2 cut(s)	600, 677
BpmI	CTGGAG	1 cut(s)	360
Bpu10I	CCTNAGC	1 cut(s)	203
BpuEI	CTTGAG	1 cut(s)	260
BpuMI	CCSGG	1 cut(s)	998
BsaAI	YACGTR	1 cut(s)	2379
BsaHI	GRCGYC	2 cut(s)	5, 147
BsaI	GGTCTC	1 cut(s)	2327
BsaJI	CCNNGG	5 cut(s)	814, 996, 1300, 1875, 2161
BsaWI	WCCGGW	2 cut(s)	30, 1570
BsaXI	ACNNNNNCTCC	2 cut(s)	80, 110
Bsc4I	CCNNNNNNNGG	7 cut(s)	156, 317, 341, 433, 1482, 1483, 2167
Bse118I	RCCGGY	2 cut(s)	30, 233
Bse1I	ACTGG	6 cut(s)	311, 1192, 1252, 1442, 1915, 2407
Bse3DI	GCAATG	3 cut(s)	605, 1146, 1836
BseAI	TCCGGA	1 cut(s)	1570
BseDI	CCNNGG	5 cut(s)	814, 996, 1300, 1875, 2161
BseGI	GGATG	9 cut(s)	83, 394, 457, 605, 734, 907, 1420, 1821, 2265
BseLI	CCNNNNNNNGG	7 cut(s)	156, 317, 341, 433, 1482, 1483, 2167
BseMI	GCAATG	3 cut(s)	605, 1146, 1836
BseMII	CTCAG	4 cut(s)	217, 888, 2284, 2351
BseNI	ACTGG	6 cut(s)	311, 1192, 1252, 1442, 1915, 2407
BseRI	GAGGAG	5 cut(s)	85, 2000, 2315, 2458, 2531
BseSI	GKGCMC	1 cut(s)	42
BseXI	GCAGC	7 cut(s)	411, 650, 938, 1484, 1926, 1929, 2564
BshFI	GGCC	6 cut(s)	69, 200, 310, 426, 1691, 2080
BshTI	ACCGGT	1 cut(s)	30
BsiSI	CCGG	6 cut(s)	31, 63, 234, 360, 998, 1571
BslFI	GGGAC	5 cut(s)	275, 533, 659, 1030, 2503
BslI	CCNNNNNNNGG	7 cut(s)	156, 317, 341, 433, 1482, 1483, 2167
BsmAI	GTCTC	5 cut(s)	12, 85, 2120, 2327, 2420
BsmBI	CGTCTC	2 cut(s)	12, 85
BsmFI	GGGAC	5 cut(s)	275, 533, 659, 1030, 2503
BsmI	GAATGC	1 cut(s)	1838
BsnI	GGCC	6 cut(s)	69, 200, 310, 426, 1691, 2080
Bso31I	GGTCTC	1 cut(s)	2327
Bsp1286I	GDGCHC	1 cut(s)	42
Bsp13I	TCCGGA	1 cut(s)	1570
Bsp143I	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
BspACI	CCGC	8 cut(s)	66, 114, 192, 231, 489, 924, 1357, 2474
BspANI	GGCC	6 cut(s)	69, 200, 310, 426, 1691, 2080
BspCNI	CTCAG	4 cut(s)	216, 887, 2283, 2350
BspEI	TCCGGA	1 cut(s)	1570
BspHI	TCATGA	1 cut(s)	301
BspLI	GGNNCC	8 cut(s)	70, 94, 263, 309, 647, 748, 1771, 2593
BspMI	ACCTGC	1 cut(s)	1169
BspPI	GGATC	3 cut(s)	458, 1551, 1581
BspQI	GCTCTTC	1 cut(s)	230
BspTI	CTTAAG	1 cut(s)	1595
BspTNI	GGTCTC	1 cut(s)	2327
BsrDI	GCAATG	3 cut(s)	605, 1146, 1836
BsrFI	RCCGGY	2 cut(s)	30, 233
BsrI	ACTGG	6 cut(s)	311, 1192, 1252, 1442, 1915, 2407
BssAI	RCCGGY	2 cut(s)	30, 233
BssECI	CCNNGG	5 cut(s)	814, 996, 1300, 1875, 2161
BssMI	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
BssNI	GRCGYC	2 cut(s)	5, 147
BssSI	CACGAG	2 cut(s)	1764, 2436
BssT1I	CCWWGG	3 cut(s)	1300, 1875, 2161
Bst2BI	CACGAG	2 cut(s)	1764, 2436
Bst4CI	ACNGT	9 cut(s)	54, 242, 508, 571, 676, 805, 815, 1267, 1812
Bst6I	CTCTTC	6 cut(s)	230, 458, 616, 2114, 2166, 2541
BstACI	GRCGYC	2 cut(s)	5, 147
BstAFI	CTTAAG	1 cut(s)	1595
BstAPI	GCANNNNNTGC	2 cut(s)	405, 1296
BstBAI	YACGTR	1 cut(s)	2379
BstC8I	GCNNGC	7 cut(s)	38, 106, 284, 351, 493, 1905, 2078
BstDEI	CTNAG	6 cut(s)	178, 203, 551, 874, 2270, 2337
BstDSI	CCRYGG	1 cut(s)	814
BstEII	GGTNACC	1 cut(s)	809
BstENI	CCTNNNNNAGG	1 cut(s)	2165
BstF5I	GGATG	9 cut(s)	83, 394, 457, 605, 734, 907, 1420, 1821, 2265
BstHHI	GCGC	1 cut(s)	710
BstKTI	GATC	9 cut(s)	132, 466, 976, 1390, 1546, 1576, 1645, 1852, 2178
BstMAI	GTCTC	5 cut(s)	12, 85, 2120, 2327, 2420
BstMBI	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
BstPI	GGTNACC	1 cut(s)	809
BstSCI	CCNGG	1 cut(s)	996
BstSFI	CTRYAG	2 cut(s)	658, 1623
BstSLI	GKGCMC	1 cut(s)	42
BstV1I	GCAGC	7 cut(s)	411, 650, 938, 1484, 1926, 1929, 2564
BstV2I	GAAGAC	2 cut(s)	600, 677
BstX2I	RGATCY	1 cut(s)	1573
BstYI	RGATCY	1 cut(s)	1573
BsuRI	GGCC	6 cut(s)	69, 200, 310, 426, 1691, 2080
BtgI	CCRYGG	1 cut(s)	814
BtgZI	GCGATG	3 cut(s)	85, 510, 1935
BtsCI	GGATG	9 cut(s)	83, 394, 457, 605, 734, 907, 1420, 1821, 2265
BtsI	GCAGTG	1 cut(s)	1548
BtsIMutI	CAGTG	4 cut(s)	318, 1263, 1449, 1548
BveI	ACCTGC	1 cut(s)	1169
Cac8I	GCNNGC	7 cut(s)	38, 106, 284, 351, 493, 1905, 2078
CaiI	CAGNNNCTG	3 cut(s)	1502, 1664, 1793
CciI	TCATGA	1 cut(s)	301
CfoI	GCGC	1 cut(s)	710
Cfr10I	RCCGGY	2 cut(s)	30, 233
Cfr13I	GGNCC	7 cut(s)	68, 159, 199, 262, 308, 646, 1689
CseI	GACGC	2 cut(s)	155, 1140
Csp6I	GTAC	5 cut(s)	1133, 1392, 1580, 1592, 1950
CspAI	ACCGGT	1 cut(s)	30
CspCI	CAANNNNNGTGG	2 cut(s)	1105, 1140
CviQI	GTAC	5 cut(s)	1133, 1392, 1580, 1592, 1950
DdeI	CTNAG	6 cut(s)	178, 203, 551, 874, 2270, 2337
DpnI	GATC	9 cut(s)	131, 465, 975, 1389, 1545, 1575, 1644, 1851, 2177
DpnII	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
DraI	TTTAAA	3 cut(s)	421, 2001, 2542
DraIII	CACNNNGTG	1 cut(s)	2245
DrdI	GACNNNNNNGTC	1 cut(s)	2407
DriI	GACNNNNNGTC	1 cut(s)	1150
DseDI	GACNNNNNNGTC	1 cut(s)	2407
Eam1104I	CTCTTC	6 cut(s)	230, 458, 616, 2114, 2166, 2541
Eam1105I	GACNNNNNGTC	1 cut(s)	1150
EarI	CTCTTC	6 cut(s)	230, 458, 616, 2114, 2166, 2541
EciI	GGCGGA	1 cut(s)	220
Eco130I	CCWWGG	3 cut(s)	1300, 1875, 2161
Eco147I	AGGCCT	1 cut(s)	2080
Eco31I	GGTCTC	1 cut(s)	2327
Eco32I	GATATC	1 cut(s)	1489
Eco47I	GGWCC	3 cut(s)	159, 262, 646
Eco57I	CTGAAG	6 cut(s)	612, 861, 876, 927, 1770, 2529
Eco91I	GGTNACC	1 cut(s)	809
EcoNI	CCTNNNNNAGG	1 cut(s)	2165
EcoO109I	RGGNCCY	2 cut(s)	262, 646
EcoO65I	GGTNACC	1 cut(s)	809
EcoRI	GAATTC	1 cut(s)	835
EcoRV	GATATC	1 cut(s)	1489
EcoT14I	CCWWGG	3 cut(s)	1300, 1875, 2161
EcoT22I	ATGCAT	1 cut(s)	1838
ErhI	CCWWGG	3 cut(s)	1300, 1875, 2161
Esp3I	CGTCTC	2 cut(s)	12, 85
FalI	AAGNNNNNCTT	6 cut(s)	775, 807, 942, 974, 2139, 2171
FaqI	GGGAC	5 cut(s)	275, 533, 659, 1030, 2503
FauNDI	CATATG	1 cut(s)	685
FbaI	TGATCA	3 cut(s)	973, 1642, 1849
Fnu4HI	GCNGC	9 cut(s)	67, 115, 400, 664, 927, 1473, 1915, 1918, 2553
FokI	GGATG	9 cut(s)	70, 401, 444, 612, 721, 914, 1427, 1828, 2252
Fsp4HI	GCNGC	9 cut(s)	67, 115, 400, 664, 927, 1473, 1915, 1918, 2553
FspBI	CTAG	2 cut(s)	444, 2015
FspI	TGCGCA	1 cut(s)	709
GlaI	GCGC	1 cut(s)	709
GluI	GCNGC	9 cut(s)	67, 115, 400, 664, 927, 1473, 1915, 1918, 2553
GsuI	CTGGAG	1 cut(s)	360
HaeIII	GGCC	6 cut(s)	69, 200, 310, 426, 1691, 2080
HapII	CCGG	6 cut(s)	31, 63, 234, 360, 998, 1571
HgaI	GACGC	2 cut(s)	155, 1140
HhaI	GCGC	1 cut(s)	710
Hin1I	GRCGYC	2 cut(s)	5, 147
Hin6I	GCGC	1 cut(s)	708
HinP1I	GCGC	1 cut(s)	708
HincII	GTYRAC	1 cut(s)	528
HindII	GTYRAC	1 cut(s)	528
HindIII	AAGCTT	2 cut(s)	1971, 2087
HinfI	GANTC	8 cut(s)	119, 137, 154, 905, 918, 1280, 1447, 2005
HpaI	GTTAAC	1 cut(s)	528
HpaII	CCGG	6 cut(s)	31, 63, 234, 360, 998, 1571
HphI	GGTGA	5 cut(s)	323, 821, 913, 1679, 2398
Hpy166II	GTNNAC	4 cut(s)	162, 528, 1538, 2389
Hpy8I	GTNNAC	4 cut(s)	162, 528, 1538, 2389
Hpy99I	CGWCG	2 cut(s)	92, 215
HpyCH4III	ACNGT	9 cut(s)	54, 242, 508, 571, 676, 805, 815, 1267, 1812
HpyCH4IV	ACGT	3 cut(s)	1019, 2378, 2536
HpyF3I	CTNAG	6 cut(s)	178, 203, 551, 874, 2270, 2337
HpySE526I	ACGT	3 cut(s)	1019, 2378, 2536
Hsp92I	GRCGYC	2 cut(s)	5, 147
HspAI	GCGC	1 cut(s)	708
Kpn2I	TCCGGA	1 cut(s)	1570
Ksp22I	TGATCA	3 cut(s)	973, 1642, 1849
KspAI	GTTAAC	1 cut(s)	528
Kzo9I	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
LguI	GCTCTTC	1 cut(s)	230
LmnI	GCTCC	5 cut(s)	98, 362, 1251, 1334, 2518
Lsp1109I	GCAGC	7 cut(s)	411, 650, 938, 1484, 1926, 1929, 2564
LweI	GCATC	3 cut(s)	1760, 1912, 2023
MaeI	CTAG	2 cut(s)	444, 2015
MaeII	ACGT	3 cut(s)	1019, 2378, 2536
MalI	GATC	9 cut(s)	131, 465, 975, 1389, 1545, 1575, 1644, 1851, 2177
MboI	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
MflI	RGATCY	1 cut(s)	1573
MhlI	GDGCHC	1 cut(s)	42
MlyI	GAGTC	1 cut(s)	128
MmeI	TCCRAC	4 cut(s)	111, 1002, 1864, 2300
Mph1103I	ATGCAT	1 cut(s)	1838
MroI	TCCGGA	1 cut(s)	1570
MroXI	GAANNNNTTC	3 cut(s)	1364, 1974, 2196
MslI	CAYNNNNRTG	2 cut(s)	1400, 1934
MspA1I	CMGCKG	1 cut(s)	617
MspCI	CTTAAG	1 cut(s)	1595
MspI	CCGG	6 cut(s)	31, 63, 234, 360, 998, 1571
MspR9I	CCNGG	1 cut(s)	998
Mva1269I	GAATGC	1 cut(s)	1838
NciI	CCSGG	1 cut(s)	998
NdeI	CATATG	1 cut(s)	685
NdeII	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
NlaIV	GGNNCC	8 cut(s)	70, 94, 263, 309, 647, 748, 1771, 2593
NmuCI	GTSAC	7 cut(s)	236, 329, 809, 880, 964, 1243, 1316
NsbI	TGCGCA	1 cut(s)	709
NsiI	ATGCAT	1 cut(s)	1838
PagI	TCATGA	1 cut(s)	301
PceI	AGGCCT	1 cut(s)	2080
PciSI	GCTCTTC	1 cut(s)	230
PctI	GAATGC	1 cut(s)	1838
PdmI	GAANNNNTTC	3 cut(s)	1364, 1974, 2196
PfeI	GAWTC	7 cut(s)	137, 154, 905, 918, 1280, 1447, 2005
PinAI	ACCGGT	1 cut(s)	30
PkrI	GCNGC	9 cut(s)	68, 116, 401, 665, 928, 1474, 1916, 1919, 2554
PleI	GAGTC	1 cut(s)	127
PpsI	GAGTC	1 cut(s)	127
Ppu21I	YACGTR	1 cut(s)	2379
PpuMI	RGGWCCY	2 cut(s)	262, 646
PshBI	ATTAAT	2 cut(s)	1586, 2286
PsiI	TTATAA	3 cut(s)	800, 1655, 2094
Psp1406I	AACGTT	1 cut(s)	2536
Psp5II	RGGWCCY	2 cut(s)	262, 646
PspEI	GGTNACC	1 cut(s)	809
PspN4I	GGNNCC	8 cut(s)	70, 94, 263, 309, 647, 748, 1771, 2593
PspPI	GGNCC	7 cut(s)	68, 159, 199, 262, 308, 646, 1689
PspPPI	RGGWCCY	2 cut(s)	262, 646
PstNI	CAGNNNCTG	3 cut(s)	1502, 1664, 1793
PsuI	RGATCY	1 cut(s)	1573
PvuII	CAGCTG	1 cut(s)	617
RsaI	GTAC	5 cut(s)	1134, 1393, 1581, 1593, 1951
RsaNI	GTAC	5 cut(s)	1133, 1392, 1580, 1592, 1950
RseI	CAYNNNNRTG	2 cut(s)	1400, 1934
SapI	GCTCTTC	1 cut(s)	230
SatI	GCNGC	9 cut(s)	67, 115, 400, 664, 927, 1473, 1915, 1918, 2553
Sau3AI	GATC	9 cut(s)	129, 463, 973, 1387, 1543, 1573, 1642, 1849, 2175
Sau96I	GGNCC	7 cut(s)	68, 159, 199, 262, 308, 646, 1689
SchI	GAGTC	1 cut(s)	128
ScrFI	CCNGG	1 cut(s)	998
SduI	GDGCHC	1 cut(s)	42
SfaNI	GCATC	3 cut(s)	1760, 1912, 2023
SfcI	CTRYAG	2 cut(s)	658, 1623
SinI	GGWCC	3 cut(s)	159, 262, 646
SmiMI	CAYNNNNRTG	2 cut(s)	1400, 1934
SmlI	CTYRAG	2 cut(s)	275, 1595
SmoI	CTYRAG	2 cut(s)	275, 1595
SseBI	AGGCCT	1 cut(s)	2080
SsiI	CCGC	8 cut(s)	66, 114, 192, 231, 489, 924, 1357, 2474
SspMI	CTAG	2 cut(s)	444, 2015
StuI	AGGCCT	1 cut(s)	2080
StyD4I	CCNGG	1 cut(s)	996
StyI	CCWWGG	3 cut(s)	1300, 1875, 2161
TaaI	ACNGT	9 cut(s)	54, 242, 508, 571, 676, 805, 815, 1267, 1812
TaiI	ACGT	3 cut(s)	1022, 2381, 2539
TaqI	TCGA	2 cut(s)	152, 2418
TaqII	GACCGA	1 cut(s)	147
TatI	WGTACW	2 cut(s)	1591, 1949
TauI	GCSGC	2 cut(s)	69, 117
TfiI	GAWTC	7 cut(s)	137, 154, 905, 918, 1280, 1447, 2005
TscAI	CASTG	4 cut(s)	318, 1270, 1449, 1555
TseFI	GTSAC	7 cut(s)	236, 329, 809, 880, 964, 1243, 1316
TseI	GCWGC	7 cut(s)	399, 663, 926, 1472, 1914, 1917, 2552
Tsp45I	GTSAC	7 cut(s)	236, 329, 809, 880, 964, 1243, 1316
TspDTI	ATGAA	5 cut(s)	129, 683, 918, 1091, 1845
TspGWI	ACGGA	2 cut(s)	612, 2581
TspRI	CASTG	4 cut(s)	318, 1270, 1449, 1555
Vha464I	CTTAAG	1 cut(s)	1595
VpaK11BI	GGWCC	3 cut(s)	159, 262, 646
VspI	ATTAAT	2 cut(s)	1586, 2286
XagI	CCTNNNNNAGG	1 cut(s)	2165
XapI	RAATTY	3 cut(s)	835, 1025, 2109
XcmI	CCANNNNNNNNNTGG	1 cut(s)	130
XmnI	GAANNNNTTC	3 cut(s)	1364, 1974, 2196
XspI	CTAG	2 cut(s)	444, 2015
Zsp2I	ATGCAT	1 cut(s)	1838

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.