Rh1BG429400

Transducin beta-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010104
Physical Location & Seq
Forward (+)
54625733 .. 54631237
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG429400.1

Sequence Viewer

Length: 2628 bp
ATGGCGTCTCTACCTTTGAAGAAGAACTACAGGTGCGTCCCCTCTCTGCAACCGTTCTACTCCGGCGGCGCCTTTGCCGTCTCATCCGACGGTTCCTTCATCGCCTGCGCTTCCGGCGAGTCCATTAACATCGTCGATGCCTCCAACTCCTCTGTACGCTCCACTCTGGAAGGCGACTCCGAGGCCGTCACTGCTTTAACCCTCAGCCCCGACGACAAGTTGCTCTTCTCGTCCGGCCACAGTCGCCTCATTAGGGTTTGGGACCTCTCCACCTTCAAATGCGTCCGCTCTTGGAAGGGTCATGATGGGCCTGTGATGGGAATGGCTTGTCACCCATCCGGCGGATATCTTGCCACCGCCGGAGCCGATAAGAAGGTTATAGTTTGGGATGTTGCCGGTAGCTTTGCCACTCATCACTTTAAAGGTCACCAAGGGGTTGTTTCTAGTCTTCTCTTTCATCCAGATCCTACCAAGCAAATTCTTTTCTCCGCAAGTCATGATACAACTGTGAACGTCTGGGATCTAGATACTAAGAAGCGTGTCGCAACCCTTACTGAGCATAACTCAACGGTAACTTCTATGGCATTATCCGAAGATGGGCGCACGTTGCTCACAGCTGGAAGAGATAAGGTTGTGATCTTGTGGAACCTTGATGACTACAGCTGCTTGAAGACTGTAACAACATATGAAGCCCTTGAAGCTGTGTGTGTAATTCCTTCTGGAACTCTTTTATCTTCCCGTTTCGGTTCATCCAAGCAGCGGAATGGGAAGAAAAGCAAGTCACCTGCAATATGTTTCATAACAGTTGGTGACCGTGGGATCGTACGAATTTGGAATTCTGAAGGTGCAGTTTGCCTATTTGAGCAGAAGTCCTCAGATGTTACTATCACCTCTGATGACGATGAATCAAAAAGGGGCTTTACTGCAGCTTTACTGCTTCCATCAGATCAAGGGCTGCTTTGTGTGACAGCTGATCAGGAGTTCCTTTTATATTCTCCTGTTGAAAGCCCTGAAGGGACCTTTGAATTTGTTCTAAGCAAAAGACTTGTAGGAAATAATGGAGAGATTCTGGATATGAAGTTTCTAGGTGATGAGGAACAATTTATCGCTGTTGCCACAAATACTGAACAGGTACAAGTGTTTGATGTTGCATCCATGTCATGTTCTCACGTATTGGCCGGTCATACTGAAATTGTTATGTGCCTTGACACCTGCGTATCAAGTTCCGGAAGAACACTTATAGTGACAGGAAGCAAGGATAATAGTGTTAGGTTGTGGGAATCAGAAAGCAGATGTTGCCTTGGTGTTGGCGTAGGTCACATGGGATCTGTTGGAGCTATTGCTTTCTCAAAGAAGCAGAGGGACTTCTTTGTCAGTGGTAGTAGTGATCGTACCCTCAAGGTCTGGAGTTTGGATGGTATCTCAGACAATGTGGAAAAGCCAATAAATTTGAAAGCAAAAGCTGTTGTAGCAGCCCACGATAAGGATATCAATTCAGTAGCTGTTGCACCAAATGATAGTTTAGTTTGTAGTGGTTCACAGGACCGCACTGCTTGTGTTTGGAGGCTTCCAGATCTTGTACCAGTAATTGTACTTAAGGGTCATAAAAGAGGGGTTTGGTCTGTAGAGTTTTCTCCAGTTGATCAATGCATTCTGACTACATCTGGTGATAAAACAATAAAGATATGGGCTATATCAGATGGCTCGTGCTTAAAATCATTTGAAGGACACATGTCAAGTGTATATCGAGCATCATTTCTTACTCGTGGGACCCAATTTATTTCTTCTGATGCTGATGGTTTGGTGAAGCTGTGGATGGTCAAAACAAATGAATGCATTGCTACATATGATCAGCATGAGGACAAGGTTTGGGCATTGACTGTTGGAAAGAAGACAGAAATGGTTGCAACTGGCAGCGGTGATGCTGCCATCAATCTGTGGTATGATTGTACTGCCTCTGATAAAGAGGAAGCTTTCCGCAGAGAGGAGGAAGGCGTCCTGAAAAGTCAAGAACTAGAAAATGCTGTCAAAGATGCTGACTATACTAAAGCAATCCAAATTGCATTTAAGCTTCGCAGGCCTCATAAACTTTATGAGTGTTTTCATGAACTTTTCAGGAAGAGAGGGGATGAAAAGCAGATAGAGAAAGCCCTTCATGACTTTGGCAACGAAGAGTTGGCGATACTTTTTGAATATGTTAGGGAATGGAATACAAAGCCAAAGTTTTGCCATGTTGCCCAGTTCGTGTTCTTTAAAATTCTCAACATTTATAGTCCAACAGAGATTATTAAGGTAAAAGGCATTCAGAATCTTCTTGAAGCTCTCACCCCATATTCTCAGAGGCACTATAGCAGAGTAGACAGGCATGTGAGAAACACACTCCTGTTGGACTTCATTCTGCACTCGATGGCAGTTGTTGAACCAGATACAGGTGCTAGAGAATTGAGTGAAGAGTCTTTGATTCCCTCTGGTGTCAATCATGAGAATGAAACACGTAGTATGGAGGCTGTTTCCGATCCTAAGCAGGCATCTGAAGGGTTGAAAGAAAAGGCAGCCTCGAGGAAACGGAAGTCAAAGAAATCAAAAGATGGTTCAAAGAAGAAAGCTAAAGCCTCCAGCTTAGAATAG

Protein Analysis

875

Amino Acids

96.17

Weight (kDa)

6.42

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 25 - 100 9.9e-10 WDHD1 first WD40 domain
Beta-prop_DCAF4 PF23761 25 - 131 3.2e-07 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR5 PF25175 26 - 156 1.6e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 26 - 130 1.7e-11 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 26 - 155 1.3e-19 THOC3 beta-propeller domain
WDR55 PF24796 27 - 130 3.1e-06 WDR55
WD40_CDC20-Fz PF24807 30 - 246 7.8e-27 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 32 - 245 2.1e-37 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 33 - 217 1.1e-39 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 33 - 218 9.4e-16 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 41 - 245 1.9e-20 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 45 - 221 2.8e-17 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 55 - 239 4.6e-37 WDR5 beta-propeller domain
EIF3I PF24805 56 - 139 4.2e-06 EIF3I
Beta-prop_EML_2 PF23414 64 - 203 7.2e-17 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 73 - 175 6.8e-11 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 83 - 226 3.6e-07 SCAP Beta-propeller
WD40 PF00400 93 - 130 3.9e-09 WD domain, G-beta repeat
Beta-prop_EML PF23409 96 - 232 1.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 99 - 246 1.9e-15 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 104 - 184 3.6e-10 WDHD1 first WD40 domain
WD40_MABP1-WDR62_1st PF24780 108 - 226 2.6e-09 MABP1/WDR62 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 111 - 219 4.2e-11 WDR90/POC16, second beta-propeller
WDR55 PF24796 112 - 225 8.1e-11 WDR55
Beta-prop_WDR41 PF25178 117 - 220 2e-07 WDR41
Beta-prop_WDR3_1st PF25173 138 - 244 3.6e-19 WDR3 first beta-propeller domain
WD40 PF00400 138 - 174 3.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 140 - 216 4e-11 WDR3 second beta-propeller domain
EIF3I PF24805 141 - 388 4.8e-10 EIF3I
Beta-prop_Aladin PF25460 143 - 244 6.3e-07 Aladin seven-bladed propeller
Beta-prop_IFT140_1st PF23383 146 - 224 2.4e-06 IFT140 first beta-propeller
WD40_WDHD1_1st PF24817 156 - 233 3.2e-10 WDHD1 first WD40 domain
WD40 PF00400 179 - 216 3.2e-07 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 256 - 606 1.4e-16 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 263 - 522 2.4e-19 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 313 - 471 6.8e-19 WDR5 beta-propeller domain
WD40_Gbeta PF25391 319 - 471 8.1e-09 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 349 - 474 4.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 351 - 472 2.1e-17 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 352 - 472 7.9e-15 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 353 - 474 4e-11 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 354 - 472 3.4e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 367 - 472 6.3e-09 WDHD1 first WD40 domain
WD40 PF00400 387 - 426 4e-07 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 414 - 609 3.4e-16 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 431 - 574 7.6e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 439 - 648 7.6e-29 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 448 - 625 1.2e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 458 - 648 1e-29 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 484 - 647 2.5e-22 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 485 - 575 1.5e-11 WDHD1 first WD40 domain
WD40_Prp19 PF24814 486 - 647 5.2e-24 Prp19 WD40 domain
Beta-prop_SCAP PF24017 486 - 606 4.2e-09 SCAP Beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 488 - 571 1.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 488 - 647 2.2e-32 WDR3 first beta-propeller domain
WD40 PF00400 489 - 521 3.2e-06 WD domain, G-beta repeat
EIF3I PF24805 490 - 588 3.1e-10 EIF3I
Beta-prop_WDR5 PF25175 491 - 649 2.3e-34 WDR5 beta-propeller domain
Beta-prop_EML PF23409 492 - 657 2e-12 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 492 - 652 3.4e-07 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 494 - 646 3.7e-14 WDR55
WD40 PF00400 528 - 563 3.6e-08 WD domain, G-beta repeat
Beta-prop_WDR90_POC16_2nd PF23393 548 - 655 8.3e-06 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 548 - 652 3.2e-13 G protein beta WD-40 repeat protein
EIF3I PF24805 574 - 648 2.1e-08 EIF3I
Utp13 PF08625 670 - 803 2.1e-41 Utp13 specific WD40 associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 793, 1220
AasI GACNNNNNNGTC 1 cut(s) 1370
AbsI CCTCGAGG 1 cut(s) 2557
Acc36I ACCTGC 2 cut(s) 793, 1220
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 2358
AccIII TCCGGA 1 cut(s) 1226
AciI CCGC 9 cut(s) 66, 286, 342, 357, 489, 760, 1546, 1917, 1978
AclWI GGATC 5 cut(s) 458, 528, 827, 1333, 2510
AcoI YGGCCR 2 cut(s) 235, 1176
AcsI RAATTY 6 cut(s) 477, 828, 835, 1025, 1447, 2256
AcuI CTGAAG 3 cut(s) 861, 1032, 2553
AcyI GRCGYC 3 cut(s) 5, 69, 1995
AfaI GTAC 7 cut(s) 156, 825, 1134, 1393, 1581, 1593, 1951
AfiI CCNNNNNNNGG 8 cut(s) 253, 317, 341, 597, 759, 1483, 1984, 2429
AflII CTTAAG 1 cut(s) 1595
AflIII ACRYGT 2 cut(s) 1731, 2492
Alw26I GTCTC 2 cut(s) 12, 85
AlwI GGATC 5 cut(s) 458, 528, 827, 1333, 2510
AlwNI CAGNNNCTG 1 cut(s) 1502
Ama87I CYCGRG 1 cut(s) 2557
Aor13HI TCCGGA 1 cut(s) 1226
AoxI GGCC 5 cut(s) 183, 235, 308, 1176, 2078
ApeKI GCWGC 8 cut(s) 663, 757, 926, 955, 1472, 1914, 1925, 2552
ApoI RAATTY 6 cut(s) 477, 828, 835, 1025, 1447, 2256
Asp700I GAANNNNTTC 1 cut(s) 1029
AspLEI GCGC 3 cut(s) 71, 110, 603
AspS9I GGNCC 5 cut(s) 262, 308, 1017, 1543, 1770
AvaI CYCGRG 1 cut(s) 2557
AvaII GGWCC 4 cut(s) 262, 1017, 1543, 1770
BaeI ACNNNNGTAYC 6 cut(s) 492, 525, 1124, 1157, 1200, 1233
BanI GGYRCC 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BauI CACGAG 2 cut(s) 1705, 1764
BbsI GAAGAC 3 cut(s) 440, 677, 1898
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 8 cut(s) 650, 769, 938, 942, 1484, 1912, 1926, 2564
BceAI ACGGC 2 cut(s) 62, 170
BclI TGATCA 3 cut(s) 973, 1642, 1849
BcoDI GTCTC 2 cut(s) 12, 85
BfaI CTAG 5 cut(s) 444, 524, 1085, 2015, 2436
BfmI CTRYAG 5 cut(s) 28, 658, 924, 1623, 2347
BfoI RGCGCY 1 cut(s) 72
BfrI CTTAAG 1 cut(s) 1595
BfuAI ACCTGC 2 cut(s) 793, 1220
BglII AGATCT 1 cut(s) 1573
BisI GCNGC 9 cut(s) 67, 664, 758, 927, 956, 1473, 1915, 1926, 2553
BlsI GCNGC 9 cut(s) 68, 665, 759, 928, 957, 1474, 1916, 1927, 2554
Bme18I GGWCC 4 cut(s) 262, 1017, 1543, 1770
BmeT110I CYCGRG 1 cut(s) 2557
BmgT120I GGNCC 5 cut(s) 262, 308, 1017, 1543, 1770
BmiI GGNNCC 8 cut(s) 70, 94, 263, 364, 647, 1018, 1771, 1772
BmrI ACTGGG 1 cut(s) 2233
BmsI GCATC 7 cut(s) 127, 1160, 1760, 1780, 1912, 2023, 2537
BmuI ACTGGG 1 cut(s) 2233
BoxI GACNNNNGTC 1 cut(s) 1732
BpiI GAAGAC 3 cut(s) 440, 677, 1898
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 3 cut(s) 1426, 1620, 2599
Bpu10I CCTNAGC 2 cut(s) 203, 2520
BpuEI CTTGAG 1 cut(s) 1382
BsaAI YACGTR 2 cut(s) 1171, 2495
BsaHI GRCGYC 3 cut(s) 5, 69, 1995
BsaJI CCNNGG 4 cut(s) 180, 430, 814, 1300
BsaWI WCCGGW 1 cut(s) 1226
Bsc4I CCNNNNNNNGG 8 cut(s) 253, 317, 341, 597, 759, 1483, 1984, 2429
Bse118I RCCGGY 2 cut(s) 395, 1178
Bse1I ACTGG 4 cut(s) 1583, 1637, 1915, 2239
Bse3DI GCAATG 1 cut(s) 1836
BseAI TCCGGA 1 cut(s) 1226
BseDI CCNNGG 4 cut(s) 180, 430, 814, 1300
BseGI GGATG 9 cut(s) 83, 335, 394, 457, 749, 1151, 1420, 1821, 2134
BseLI CCNNNNNNNGG 8 cut(s) 253, 317, 341, 597, 759, 1483, 1984, 2429
BseMI GCAATG 1 cut(s) 1836
BseMII CTCAG 5 cut(s) 217, 546, 888, 1437, 2351
BseNI ACTGG 4 cut(s) 1583, 1637, 1915, 2239
BseRI GAGGAG 2 cut(s) 139, 2000
BseXI GCAGC 8 cut(s) 650, 769, 938, 942, 1484, 1912, 1926, 2564
BsgI GTGCAG 2 cut(s) 867, 2384
BshFI GGCC 5 cut(s) 185, 237, 310, 1178, 2080
BshNI GGYRCC 1 cut(s) 68
BsiHKCI CYCGRG 1 cut(s) 2557
BsiSI CCGG 8 cut(s) 63, 114, 234, 339, 360, 396, 1179, 1227
BsiWI CGTACG 1 cut(s) 823
BslFI GGGAC 5 cut(s) 23, 275, 1030, 1376, 1783
BslI CCNNNNNNNGG 8 cut(s) 253, 317, 341, 597, 759, 1483, 1984, 2429
BsmAI GTCTC 2 cut(s) 12, 85
BsmBI CGTCTC 2 cut(s) 12, 85
BsmFI GGGAC 5 cut(s) 23, 275, 1030, 1376, 1783
BsmI GAATGC 3 cut(s) 1650, 1838, 2301
BsnI GGCC 5 cut(s) 185, 237, 310, 1178, 2080
BsoBI CYCGRG 1 cut(s) 2557
Bsp13I TCCGGA 1 cut(s) 1226
BspACI CCGC 9 cut(s) 66, 286, 342, 357, 489, 760, 1546, 1917, 1978
BspANI GGCC 5 cut(s) 185, 237, 310, 1178, 2080
BspCNI CTCAG 5 cut(s) 216, 547, 887, 1436, 2350
BspEI TCCGGA 1 cut(s) 1226
BspHI TCATGA 5 cut(s) 301, 496, 2104, 2155, 2479
BspLI GGNNCC 8 cut(s) 70, 94, 263, 364, 647, 1018, 1771, 1772
BspMAI CTGCAG 1 cut(s) 928
BspMI ACCTGC 2 cut(s) 793, 1220
BspPI GGATC 5 cut(s) 458, 528, 827, 1333, 2510
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 68
BspTI CTTAAG 1 cut(s) 1595
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 1836
BsrFI RCCGGY 2 cut(s) 395, 1178
BsrI ACTGG 4 cut(s) 1583, 1637, 1915, 2239
BssAI RCCGGY 2 cut(s) 395, 1178
BssECI CCNNGG 4 cut(s) 180, 430, 814, 1300
BssNI GRCGYC 3 cut(s) 5, 69, 1995
BssSI CACGAG 2 cut(s) 1705, 1764
BssT1I CCWWGG 2 cut(s) 430, 1300
Bst2BI CACGAG 2 cut(s) 1705, 1764
Bst4CI ACNGT 9 cut(s) 54, 92, 242, 508, 571, 676, 805, 815, 1882
Bst6I CTCTTC 5 cut(s) 230, 616, 2114, 2166, 2445
BstACI GRCGYC 3 cut(s) 5, 69, 1995
BstAFI CTTAAG 1 cut(s) 1595
BstAPI GCANNNNNTGC 1 cut(s) 1296
BstBAI YACGTR 2 cut(s) 1171, 2495
BstC8I GCNNGC 3 cut(s) 106, 2078, 2526
BstDEI CTNAG 9 cut(s) 203, 531, 555, 874, 1034, 1423, 2337, 2520, 2620
BstDSI CCRYGG 1 cut(s) 814
BstEII GGTNACC 2 cut(s) 425, 809
BstF5I GGATG 9 cut(s) 83, 335, 394, 457, 749, 1151, 1420, 1821, 2134
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 3 cut(s) 71, 110, 603
BstMAI GTCTC 2 cut(s) 12, 85
BstMWI GCNNNNNNNGC 8 cut(s) 114, 191, 243, 607, 698, 1296, 1469, 2077
BstNSI RCATGY 2 cut(s) 1735, 2369
BstPAI GACNNNNGTC 1 cut(s) 1732
BstPI GGTNACC 2 cut(s) 425, 809
BstSFI CTRYAG 5 cut(s) 28, 658, 924, 1623, 2347
BstV1I GCAGC 8 cut(s) 650, 769, 938, 942, 1484, 1912, 1926, 2564
BstV2I GAAGAC 3 cut(s) 440, 677, 1898
BstX2I RGATCY 4 cut(s) 463, 520, 1325, 1573
BstYI RGATCY 4 cut(s) 463, 520, 1325, 1573
BsuRI GGCC 5 cut(s) 185, 237, 310, 1178, 2080
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 9 cut(s) 83, 335, 394, 457, 749, 1151, 1420, 1821, 2134
BtsI GCAGTG 2 cut(s) 189, 1548
BtsIMutI CAGTG 3 cut(s) 189, 1381, 1548
BveI ACCTGC 2 cut(s) 793, 1220
Cac8I GCNNGC 3 cut(s) 106, 2078, 2526
CaiI CAGNNNCTG 1 cut(s) 1502
CciI TCATGA 5 cut(s) 301, 496, 2104, 2155, 2479
CfoI GCGC 3 cut(s) 71, 110, 603
Cfr10I RCCGGY 2 cut(s) 395, 1178
Cfr13I GGNCC 5 cut(s) 262, 308, 1017, 1543, 1770
CseI GACGC 3 cut(s) 25, 271, 1984
Csp6I GTAC 7 cut(s) 155, 824, 1133, 1392, 1580, 1592, 1950
CviQI GTAC 7 cut(s) 155, 824, 1133, 1392, 1580, 1592, 1950
DdeI CTNAG 9 cut(s) 203, 531, 555, 874, 1034, 1423, 2337, 2520, 2620
DinI GGCGCC 1 cut(s) 70
DraI TTTAAA 2 cut(s) 421, 2254
DrdI GACNNNNNNGTC 1 cut(s) 1370
DseDI GACNNNNNNGTC 1 cut(s) 1370
EaeI YGGCCR 2 cut(s) 235, 1176
Eam1104I CTCTTC 5 cut(s) 230, 616, 2114, 2166, 2445
EarI CTCTTC 5 cut(s) 230, 616, 2114, 2166, 2445
EciI GGCGGA 1 cut(s) 357
Eco130I CCWWGG 2 cut(s) 430, 1300
Eco147I AGGCCT 1 cut(s) 2080
Eco32I GATATC 2 cut(s) 347, 1489
Eco47I GGWCC 4 cut(s) 262, 1017, 1543, 1770
Eco57I CTGAAG 3 cut(s) 861, 1032, 2553
Eco88I CYCGRG 1 cut(s) 2557
Eco91I GGTNACC 2 cut(s) 425, 809
EcoO109I RGGNCCY 3 cut(s) 262, 1017, 1770
EcoO65I GGTNACC 2 cut(s) 425, 809
EcoRI GAATTC 1 cut(s) 835
EcoRV GATATC 2 cut(s) 347, 1489
EcoT14I CCWWGG 2 cut(s) 430, 1300
EcoT22I ATGCAT 2 cut(s) 1652, 1838
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 430, 1300
Esp3I CGTCTC 2 cut(s) 12, 85
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 942, 974
FaqI GGGAC 5 cut(s) 23, 275, 1030, 1376, 1783
FauNDI CATATG 2 cut(s) 685, 1846
FbaI TGATCA 3 cut(s) 973, 1642, 1849
FblI GTMKAC 1 cut(s) 2358
Fnu4HI GCNGC 9 cut(s) 67, 664, 758, 927, 956, 1473, 1915, 1926, 2553
FokI GGATG 9 cut(s) 70, 322, 401, 444, 736, 1138, 1427, 1828, 2141
Fsp4HI GCNGC 9 cut(s) 67, 664, 758, 927, 956, 1473, 1915, 1926, 2553
FspBI CTAG 5 cut(s) 444, 524, 1085, 2015, 2436
GlaI GCGC 3 cut(s) 70, 109, 602
GluI GCNGC 9 cut(s) 67, 664, 758, 927, 956, 1473, 1915, 1926, 2553
GsuI CTGGAG 3 cut(s) 1426, 1620, 2599
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 5 cut(s) 185, 237, 310, 1178, 2080
HapII CCGG 8 cut(s) 63, 114, 234, 339, 360, 396, 1179, 1227
HgaI GACGC 3 cut(s) 25, 271, 1984
HhaI GCGC 3 cut(s) 71, 110, 603
Hin1I GRCGYC 3 cut(s) 5, 69, 1995
Hin6I GCGC 3 cut(s) 69, 108, 601
HinP1I GCGC 3 cut(s) 69, 108, 601
HindIII AAGCTT 2 cut(s) 1971, 2069
HinfI GANTC 8 cut(s) 119, 176, 905, 1066, 1280, 2308, 2453, 2461
HpaII CCGG 8 cut(s) 63, 114, 234, 339, 360, 396, 1179, 1227
Hpy166II GTNNAC 3 cut(s) 511, 1538, 2359
Hpy8I GTNNAC 3 cut(s) 511, 1538, 2359
Hpy99I CGWCG 3 cut(s) 92, 137, 215
HpyCH4III ACNGT 9 cut(s) 54, 92, 242, 508, 571, 676, 805, 815, 1882
HpyCH4IV ACGT 4 cut(s) 513, 605, 1170, 2494
HpyF10VI GCNNNNNNNGC 8 cut(s) 114, 191, 243, 607, 698, 1296, 1469, 2077
HpyF3I CTNAG 9 cut(s) 203, 531, 555, 874, 1034, 1423, 2337, 2520, 2620
HpySE526I ACGT 4 cut(s) 513, 605, 1170, 2494
Hsp92I GRCGYC 3 cut(s) 5, 69, 1995
HspAI GCGC 3 cut(s) 69, 108, 601
KasI GGCGCC 1 cut(s) 68
KflI GGGWCCC 1 cut(s) 1770
Kpn2I TCCGGA 1 cut(s) 1226
Ksp22I TGATCA 3 cut(s) 973, 1642, 1849
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 3 cut(s) 164, 362, 1334
Lsp1109I GCAGC 8 cut(s) 650, 769, 938, 942, 1484, 1912, 1926, 2564
LweI GCATC 7 cut(s) 127, 1160, 1760, 1780, 1912, 2023, 2537
MaeI CTAG 5 cut(s) 444, 524, 1085, 2015, 2436
MaeII ACGT 4 cut(s) 513, 605, 1170, 2494
MbiI CCGCTC 1 cut(s) 288
MflI RGATCY 4 cut(s) 463, 520, 1325, 1573
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 3 cut(s) 128, 170, 2462
MmeI TCCRAC 6 cut(s) 111, 168, 1312, 1864, 2300, 2367
Mph1103I ATGCAT 2 cut(s) 1652, 1838
MroI TCCGGA 1 cut(s) 1226
MroXI GAANNNNTTC 1 cut(s) 1029
MseI TTAA 8 cut(s) 126, 197, 420, 1596, 1712, 2067, 2253, 2289
MslI CAYNNNNRTG 1 cut(s) 2484
MspA1I CMGCKG 5 cut(s) 617, 663, 760, 971, 1917
MspCI CTTAAG 1 cut(s) 1595
MspI CCGG 8 cut(s) 63, 114, 234, 339, 360, 396, 1179, 1227
Mva1269I GAATGC 3 cut(s) 1650, 1838, 2301
MwoI GCNNNNNNNGC 8 cut(s) 114, 191, 243, 607, 698, 1296, 1469, 2077
NarI GGCGCC 1 cut(s) 69
NdeI CATATG 2 cut(s) 685, 1846
NlaIV GGNNCC 8 cut(s) 70, 94, 263, 364, 647, 1018, 1771, 1772
NmuCI GTSAC 8 cut(s) 187, 329, 425, 780, 809, 964, 1243, 1316
NsiI ATGCAT 2 cut(s) 1652, 1838
NspI RCATGY 2 cut(s) 1735, 2369
PaeR7I CTCGAG 1 cut(s) 2557
PagI TCATGA 5 cut(s) 301, 496, 2104, 2155, 2479
PaqCI CACCTGC 2 cut(s) 793, 1220
PceI AGGCCT 1 cut(s) 2080
PciI ACATGT 1 cut(s) 1731
PciSI GCTCTTC 1 cut(s) 230
PctI GAATGC 3 cut(s) 1650, 1838, 2301
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 5 cut(s) 905, 1066, 1280, 2308, 2461
Pfl23II CGTACG 1 cut(s) 823
PkrI GCNGC 9 cut(s) 68, 665, 759, 928, 957, 1474, 1916, 1927, 2554
PleI GAGTC 3 cut(s) 127, 170, 2461
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 3 cut(s) 127, 170, 2461
Ppu21I YACGTR 2 cut(s) 1171, 2495
PpuMI RGGWCCY 3 cut(s) 262, 1017, 1770
PscI ACATGT 1 cut(s) 1731
PshAI GACNNNNGTC 1 cut(s) 1732
Psp5II RGGWCCY 3 cut(s) 262, 1017, 1770
PspEI GGTNACC 2 cut(s) 425, 809
PspLI CGTACG 1 cut(s) 823
PspN4I GGNNCC 8 cut(s) 70, 94, 263, 364, 647, 1018, 1771, 1772
PspPI GGNCC 5 cut(s) 262, 308, 1017, 1543, 1770
PspPPI RGGWCCY 3 cut(s) 262, 1017, 1770
PspXI VCTCGAGB 1 cut(s) 2557
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 1502
PsuI RGATCY 4 cut(s) 463, 520, 1325, 1573
PvuII CAGCTG 3 cut(s) 617, 663, 971
RsaI GTAC 7 cut(s) 156, 825, 1134, 1393, 1581, 1593, 1951
RsaNI GTAC 7 cut(s) 155, 824, 1133, 1392, 1580, 1592, 1950
RseI CAYNNNNRTG 1 cut(s) 2484
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 8 cut(s) 126, 197, 420, 1596, 1712, 2067, 2253, 2289
SatI GCNGC 9 cut(s) 67, 664, 758, 927, 956, 1473, 1915, 1926, 2553
Sau96I GGNCC 5 cut(s) 262, 308, 1017, 1543, 1770
SchI GAGTC 3 cut(s) 128, 170, 2462
SfaNI GCATC 7 cut(s) 127, 1160, 1760, 1780, 1912, 2023, 2537
SfcI CTRYAG 5 cut(s) 28, 658, 924, 1623, 2347
SfoI GGCGCC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 2557
SinI GGWCC 4 cut(s) 262, 1017, 1543, 1770
SlaI CTCGAG 1 cut(s) 2557
SmiMI CAYNNNNRTG 1 cut(s) 2484
SmlI CTYRAG 3 cut(s) 1397, 1595, 2557
SmoI CTYRAG 3 cut(s) 1397, 1595, 2557
SseBI AGGCCT 1 cut(s) 2080
SsiI CCGC 9 cut(s) 66, 286, 342, 357, 489, 760, 1546, 1917, 1978
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 5 cut(s) 444, 524, 1085, 2015, 2436
StuI AGGCCT 1 cut(s) 2080
StyI CCWWGG 2 cut(s) 430, 1300
TaaI ACNGT 9 cut(s) 54, 92, 242, 508, 571, 676, 805, 815, 1882
TaiI ACGT 4 cut(s) 516, 608, 1173, 2497
TaqI TCGA 4 cut(s) 135, 1747, 2405, 2558
TatI WGTACW 2 cut(s) 1591, 1949
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 5 cut(s) 905, 1066, 1280, 2308, 2461
Tru1I TTAA 8 cut(s) 126, 197, 420, 1596, 1712, 2067, 2253, 2289
Tru9I TTAA 8 cut(s) 126, 197, 420, 1596, 1712, 2067, 2253, 2289
TscAI CASTG 3 cut(s) 196, 1381, 1555
TseFI GTSAC 8 cut(s) 187, 329, 425, 780, 809, 964, 1243, 1316
TseI GCWGC 8 cut(s) 663, 757, 926, 955, 1472, 1914, 1925, 2552
Tsp45I GTSAC 8 cut(s) 187, 329, 425, 780, 809, 964, 1243, 1316
TspGWI ACGGA 1 cut(s) 2581
TspRI CASTG 3 cut(s) 196, 1381, 1555
Vha464I CTTAAG 1 cut(s) 1595
VpaK11BI GGWCC 4 cut(s) 262, 1017, 1543, 1770
XapI RAATTY 6 cut(s) 477, 828, 835, 1025, 1447, 2256
XbaI TCTAGA 1 cut(s) 523
XceI RCATGY 2 cut(s) 1735, 2369
XhoI CTCGAG 1 cut(s) 2557
XmiI GTMKAC 1 cut(s) 2358
XmnI GAANNNNTTC 1 cut(s) 1029
XspI CTAG 5 cut(s) 444, 524, 1085, 2015, 2436
Zsp2I ATGCAT 2 cut(s) 1652, 1838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.