pycom07g28110

Transducin beta-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010104
Physical Location & Seq
Forward (+)
27568478 .. 27574060
5583 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28110.1

Sequence Viewer

Length: 2616 bp
ATGGCGTCTCTACCTCTGAAGAAGAACTACCGGTGCGTGCCATCTCTGCAGCAGTTCTACTCCGGCGGTCCCTTCGCCGTCTCATCCGACGGCTCATTCATCGCCTGCAAGTGCGGTGAATCCATTAAGATCGTGGATTCATCGAACGCCTCGATTCGGTCTACCATTGAAGGCGACTCAGATGAAGTTACCGCGTTGGCCCTCAGCCCCGACAACAGGCTGCTCTTCTCCGCCGGTCACAGTCGCCAAATTAGGATTTGGGACCTCTCCACCTTGAAGTGCGTGCGTTCTTGGAAGGGTCATGAGGGGCCAGTTATGGGAATGTCTTGTCACCCGTCTGGTGGATTGCTTGCGACTGCTGGAGCTGATAGGAAGGTTCTTGTTTGGGATGTTGATGGCAGCTTTTGCACTCATTACTTTAAGGGCCACAAAGGGGTCGTTTCTAGTGTTCTCTTCCATCCTGATCCTACTCAACAACTTCTTTTCTCCGCAAGCGATGATACAAATGTGCATGTTTGGGACCTATTAACCAAGAAGTGCGTTGCGACGCTTAATGCGCATAACGCAACAGTAACTTCTATGGCACTGTCTGAAGACGGATGGACTTTGCTCACAGCTGGAAGAGATAAGGTTGTAATCTTGTGGGACCTTCATGACTATAGCTGCATGAAGACTGTAACAACATATGAGGTTCTTGAAGCTGTGTGTGCAATCCATTCTGGAACTCCATTATCATCCTATTTGGGTTCCTGCAAGAAGCCTTGTGGGAAGAAAAGTGGATTACCTGAAATTTGCTTTATAACAGTTGGTGACCGTGGGATTGTACGGATTTGGAATTCTGAAGGTGCAGTTTGCCTGTATGAGCAAAAGTCCTCAGATGTTACTCTATCTTCAGATGTGGATCAATTAAATAGGGGTTTCACCGCAGCTGTCATGCTTCCTTCAGATAAAGGGTTTCTTTGTGTGACTGCTGATCAGGAGTGCCTTTTATATTCCCCAATTAAGTTGCCAGAGGGGACGCTGGAATTTGACTTGAGCAAAAGACTTGTAGGTTACAACGATGAGATTGTGGATATGAAGTTTGTCGGAGATGAGGAACAATTTCTTGCTGTTGCCACAAATCTTGAACAGGTACGAGTATATGACATTGCATCCATGTCATGTTCGTACGTATTGGCAGGTCATACCGGAATTGTTCTATGCCTTGACACCTGTGTATCAAGTTGTGGAAAACCACTTATTGTGACTGGAAGCAAAGACAACACTGTTCGGTTGTGGGATTCAGCAAGCAAATGTTGCCTTGGTCTTGGCATAGGTCATATGGGAGGTATTGGAGCTATTGCTTTCTCAAAAAAGCGGAAAGACTTCTTCGTTAGTGGTAGTAGTGATCGTACCCTCAAGGTGTGGAGTTTGGATGGTCTTCCAGACAATGCGGAAAAACCAGTGAATCTGAAAGCAAAGGCCGGTGTAGCGGCCCACGATAAGGATATTAATTCAGTAGCTGTTGCACCAAATGATAGTTTAGTTTGTAGCGGCTCACAGGATCGCACTGCTTGTGTATGGAGGCTTCCGGATCTTGTACCAGTTGTTGTACTTAAGGGTCATAAAAGGGGAGTTTGGTCTGTAGAGTTTTCTCCAGTTGATCAATGTGTTATAACGGCTTCTGGTGATAAAACAATAAAGATATGGTCCATATCTGATGGTTCATGCTTGAAAACATTTGAAGGGCATACATCAAGTGTATTAAGAGCATCATTTCTTACTCGTGGAACCCAATTTATTTCTTCAGGTGCTGATGGGTTGGTAAAACTGTGGATGGTCAAAACTGATGAATGCATTGCTACATATGATCAGCATGATGACAAGGTTTATGCCTTGGCTGTTGGAAAAAAGACAGAAATGCTTGCAACTGGCAGCAGTGATACTGTCATCAATATGTGGTATGATTGTACTGCTTCCGATAAAGAGGAAGCTTTTCGTAGGGAGGAGGAAGGTGTTTTAAAGAATCAAGAACTAGAAAATGCTGTATTAGATGCTGACTTTTCTAAAGCAATTCAAGTCGCATTTGAGCTTCGCAGGCCTCATAAGCTTTATCAGTGCTTTTCTGAAATCTGGAGGAAGAGAGAAACTGAAAAGCAGATGGAGAAAGCCCTTCTTGCCCTCGGCGAGGAAGAGATGAGGCTACTATTTGAATATGTTCGAGAATGGAATACAAAGCCAAAGCTCTGTCATGTTGCACACTTTGTGCTTTTCAAAGTTTTCAACATCCTCAGTCCAACAAAGATTAATGAGATAAAAGGCATTGGGGAAATCCTTGAAGGTCTCCTCTCGTATTCTCAGAGGCATTTTAGCAGAATGGACAGGCACGAAACAAACACGTATTTGGTGAACTACACTCTGACTGGAATGTCAGTCATTGAACCAGAGACAGATACAATAGTATTGCAGGACAAGTCGTTGATGCACTCTGCTGCGGACGATGAGAATGGGACGTTAATAAACGTACCTGAAGATGAGGAGCGAAAGACCTCTGAGCGGTTGAAAGAAACAGCAGCGACGAAAAAACGGAAGTCAAAGAAATCGAAAGATGGATCCAGTAAGAAAGTTAAGGCATAG

Protein Analysis

872

Amino Acids

95.85

Weight (kDa)

6.23

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_DCAF4 PF23761 25 - 132 1.8e-07 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_THOC3 PF25174 25 - 155 1.9e-20 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 26 - 100 1.8e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 27 - 156 1.5e-23 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 27 - 90 6.2e-06 WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz PF24807 29 - 184 1.4e-18 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 33 - 217 2.9e-38 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 34 - 215 3.6e-14 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 40 - 242 3.1e-38 Prp19 WD40 domain
Beta-prop_EML PF23409 42 - 248 8.2e-12 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd PF25047 47 - 234 4.1e-17 TEP-1 second beta-propeller
WD40 PF00400 54 - 88 2e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 71 - 155 2.5e-08 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 78 - 175 7.5e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 91 - 216 2e-19 WDR3 second beta-propeller domain
WD40 PF00400 93 - 130 2.3e-08 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 96 - 186 6.7e-07 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 96 - 226 5.4e-11 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 99 - 224 1.2e-13 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 104 - 186 2.1e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 111 - 222 3.3e-10 WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_1st PF24780 113 - 224 1.2e-06 MABP1/WDR62 first WD40 domain
Beta-prop_SCAP PF24017 114 - 216 2.9e-07 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 136 - 230 3.4e-19 WDR5 beta-propeller domain
WD40 PF00400 136 - 174 1.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 137 - 228 5.8e-20 WDR3 first beta-propeller domain
EIF3I PF24805 141 - 235 3e-07 EIF3I
Beta-prop_Aladin PF25460 147 - 236 2.8e-06 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 156 - 230 5.1e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 157 - 236 3e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 158 - 228 6.1e-08 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 162 - 244 5.2e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 265 - 522 8e-18 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 310 - 473 6.4e-10 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 312 - 471 8e-09 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 342 - 474 3.1e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 342 - 471 8.9e-18 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 350 - 472 4.2e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 352 - 472 6.7e-17 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 365 - 606 2.1e-18 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 368 - 472 2.8e-06 WDHD1 first WD40 domain
WD40 PF00400 387 - 427 5e-07 WD domain, G-beta repeat
WD40_Prp19 PF24814 399 - 472 1.1e-07 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 438 - 611 1.7e-13 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 441 - 575 5.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 443 - 648 1.5e-30 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 452 - 590 1.1e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 485 - 647 8.3e-21 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 486 - 647 8.5e-26 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 491 - 649 3.9e-37 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 491 - 650 9.8e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 491 - 605 1.1e-09 SCAP Beta-propeller
Beta-prop_WDR3_1st PF25173 491 - 647 9.4e-33 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 491 - 573 7.5e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 492 - 521 6.5e-06 WD domain, G-beta repeat
EIF3I PF24805 492 - 578 1.1e-10 EIF3I
Beta-prop_EML PF23409 492 - 586 5.2e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 493 - 576 7.8e-15 THOC3 beta-propeller domain
WDR55 PF24796 494 - 647 6.2e-13 WDR55
WD40_WDHD1_1st PF24817 494 - 574 4.7e-12 WDHD1 first WD40 domain
WD40 PF00400 528 - 564 8e-09 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 538 - 647 1.4e-07 MABP1/WDR62 second WD40 domain
Beta-prop_IFT122_1st PF23381 541 - 647 9e-06 IFT122 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 548 - 677 1.1e-07 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 548 - 652 1.4e-13 G protein beta WD-40 repeat protein
Beta-prop_SCAP PF24017 551 - 651 3e-06 SCAP Beta-propeller
WD40 PF00400 568 - 605 1.7e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 569 - 648 6e-12 THOC3 beta-propeller domain
EIF3I PF24805 575 - 650 1.3e-07 EIF3I
Utp13 PF08625 669 - 803 6.7e-39 Utp13 specific WD40 associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 800, 1655
AasI GACNNNNNNGTC 1 cut(s) 2407
Acc16I TGCGCA 1 cut(s) 558
Acc36I ACCTGC 1 cut(s) 1169
AccBSI CCGCTC 1 cut(s) 2536
AccI GTMKAC 1 cut(s) 161
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 1570
AclWI GGATC 6 cut(s) 458, 909, 1551, 1581, 2586, 2599
AcsI RAATTY 3 cut(s) 789, 835, 1025
AcuI CTGAAG 7 cut(s) 38, 612, 861, 876, 927, 1770, 2529
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 2245
AfaI GTAC 8 cut(s) 825, 1134, 1169, 1393, 1581, 1593, 1951, 2505
AfiI CCNNNNNNNGG 8 cut(s) 156, 216, 317, 341, 433, 1483, 2167, 2535
AflII CTTAAG 1 cut(s) 1595
AflIII ACRYGT 1 cut(s) 2376
AgeI ACCGGT 1 cut(s) 30
Alw26I GTCTC 4 cut(s) 12, 85, 2327, 2420
AlwI GGATC 6 cut(s) 458, 909, 1551, 1581, 2586, 2599
AlwNI CAGNNNCTG 2 cut(s) 1502, 1793
Aor13HI TCCGGA 1 cut(s) 1570
AoxI GGCC 6 cut(s) 198, 308, 424, 1461, 1473, 2078
ApeKI GCWGC 8 cut(s) 49, 220, 399, 663, 926, 1914, 2471, 2552
ApoI RAATTY 3 cut(s) 789, 835, 1025
AseI ATTAAT 2 cut(s) 1491, 2286
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 4 cut(s) 1101, 1364, 1974, 2196
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 9 cut(s) 68, 199, 262, 308, 424, 520, 646, 1474, 1689
AsuHPI GGTGA 6 cut(s) 128, 323, 821, 913, 1679, 2398
AvaII GGWCC 5 cut(s) 68, 262, 520, 646, 1689
BaeI ACNNNNGTAYC 6 cut(s) 492, 525, 1200, 1233, 2424, 2457
BamHI GGATCC 1 cut(s) 2591
BarI GAAGNNNNNNTAC 4 cut(s) 11, 43, 1551, 1583
BauI CACGAG 1 cut(s) 1764
BbsI GAAGAC 3 cut(s) 600, 677, 1412
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 8 cut(s) 61, 207, 411, 650, 938, 1926, 2458, 2564
BceAI ACGGC 3 cut(s) 62, 106, 1674
BclI TGATCA 3 cut(s) 973, 1642, 1849
BcoDI GTCTC 4 cut(s) 12, 85, 2327, 2420
BfaI CTAG 2 cut(s) 444, 2015
BfmI CTRYAG 3 cut(s) 47, 658, 1623
BfrI CTTAAG 1 cut(s) 1595
BfuAI ACCTGC 1 cut(s) 1169
Bme18I GGWCC 5 cut(s) 68, 262, 520, 646, 1689
BmgT120I GGNCC 9 cut(s) 68, 199, 262, 308, 424, 520, 646, 1474, 1689
BmiI GGNNCC 8 cut(s) 70, 263, 309, 521, 647, 748, 1771, 2593
BmsI GCATC 4 cut(s) 1160, 1760, 2023, 2451
BpiI GAAGAC 3 cut(s) 600, 677, 1412
BpmI CTGGAG 3 cut(s) 381, 1620, 2134
Bpu10I CCTNAGC 1 cut(s) 203
BpuEI CTTGAG 2 cut(s) 1054, 1382
BsaAI YACGTR 2 cut(s) 1171, 2379
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 2327
BsaJI CCNNGG 4 cut(s) 814, 1300, 1875, 2161
BsaWI WCCGGW 3 cut(s) 30, 1187, 1570
Bsc4I CCNNNNNNNGG 8 cut(s) 156, 216, 317, 341, 433, 1483, 2167, 2535
Bse118I RCCGGY 3 cut(s) 30, 233, 1463
Bse1I ACTGG 8 cut(s) 311, 1252, 1442, 1583, 1637, 1915, 2407, 2595
Bse3DI GCAATG 2 cut(s) 1146, 1836
Bse8I GATNNNNATC 1 cut(s) 900
BseAI TCCGGA 1 cut(s) 1570
BseDI CCNNGG 4 cut(s) 814, 1300, 1875, 2161
BseGI GGATG 9 cut(s) 83, 394, 457, 605, 734, 1151, 1420, 1821, 2265
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 8 cut(s) 156, 216, 317, 341, 433, 1483, 2167, 2535
BseMI GCAATG 2 cut(s) 1146, 1836
BseMII CTCAG 6 cut(s) 192, 217, 888, 2284, 2351, 2523
BseNI ACTGG 8 cut(s) 311, 1252, 1442, 1583, 1637, 1915, 2407, 2595
BseRI GAGGAG 3 cut(s) 2000, 2315, 2531
BseXI GCAGC 8 cut(s) 61, 207, 411, 650, 938, 1926, 2458, 2564
BsgI GTGCAG 1 cut(s) 867
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 6 cut(s) 200, 310, 426, 1463, 1475, 2080
BshTI ACCGGT 1 cut(s) 30
BsiSI CCGG 6 cut(s) 31, 63, 234, 1188, 1464, 1571
BsiWI CGTACG 1 cut(s) 1167
BslFI GGGAC 6 cut(s) 54, 275, 533, 659, 1030, 2503
BslI CCNNNNNNNGG 8 cut(s) 156, 216, 317, 341, 433, 1483, 2167, 2535
BsmAI GTCTC 4 cut(s) 12, 85, 2327, 2420
BsmBI CGTCTC 2 cut(s) 12, 85
BsmFI GGGAC 6 cut(s) 54, 275, 533, 659, 1030, 2503
BsmI GAATGC 1 cut(s) 1838
BsnI GGCC 6 cut(s) 200, 310, 426, 1463, 1475, 2080
Bso31I GGTCTC 1 cut(s) 2327
Bsp13I TCCGGA 1 cut(s) 1570
BspANI GGCC 6 cut(s) 200, 310, 426, 1463, 1475, 2080
BspCNI CTCAG 6 cut(s) 191, 216, 887, 2283, 2350, 2524
BspEI TCCGGA 1 cut(s) 1570
BspFNI CGCG 1 cut(s) 194
BspHI TCATGA 2 cut(s) 301, 652
BspLI GGNNCC 8 cut(s) 70, 263, 309, 521, 647, 748, 1771, 2593
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 6 cut(s) 458, 909, 1551, 1581, 2586, 2599
BspQI GCTCTTC 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 1595
BspTNI GGTCTC 1 cut(s) 2327
BsrBI CCGCTC 1 cut(s) 2536
BsrDI GCAATG 2 cut(s) 1146, 1836
BsrFI RCCGGY 3 cut(s) 30, 233, 1463
BsrI ACTGG 8 cut(s) 311, 1252, 1442, 1583, 1637, 1915, 2407, 2595
BssAI RCCGGY 3 cut(s) 30, 233, 1463
BssECI CCNNGG 4 cut(s) 814, 1300, 1875, 2161
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1764
BssT1I CCWWGG 2 cut(s) 1300, 1875
Bst2BI CACGAG 1 cut(s) 1764
Bst4CI ACNGT 9 cut(s) 242, 571, 588, 676, 805, 815, 1267, 1812, 1927
Bst6I CTCTTC 5 cut(s) 230, 458, 616, 2114, 2166
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1595
BstAPI GCANNNNNTGC 2 cut(s) 405, 1296
BstBAI YACGTR 2 cut(s) 1171, 2379
BstC8I GCNNGC 8 cut(s) 38, 106, 284, 351, 493, 1288, 1905, 2078
BstDEI CTNAG 6 cut(s) 178, 203, 874, 2270, 2337, 2532
BstDSI CCRYGG 1 cut(s) 814
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 1 cut(s) 2165
BstF5I GGATG 9 cut(s) 83, 394, 457, 605, 734, 1151, 1420, 1821, 2265
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 559
BstMAI GTCTC 4 cut(s) 12, 85, 2327, 2420
BstNSI RCATGY 1 cut(s) 515
BstPI GGTNACC 1 cut(s) 809
BstSFI CTRYAG 3 cut(s) 47, 658, 1623
BstSNI TACGTA 1 cut(s) 1171
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 8 cut(s) 61, 207, 411, 650, 938, 1926, 2458, 2564
BstV2I GAAGAC 3 cut(s) 600, 677, 1412
BstX2I RGATCY 2 cut(s) 1573, 2591
BstYI RGATCY 2 cut(s) 1573, 2591
BsuRI GGCC 6 cut(s) 200, 310, 426, 1463, 1475, 2080
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 2 cut(s) 85, 510
BtsCI GGATG 9 cut(s) 83, 394, 457, 605, 734, 1151, 1420, 1821, 2265
BtsI GCAGTG 2 cut(s) 1548, 1924
BtsIMutI CAGTG 6 cut(s) 584, 1263, 1449, 1548, 1924, 2102
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 8 cut(s) 38, 106, 284, 351, 493, 1288, 1905, 2078
CaiI CAGNNNCTG 2 cut(s) 1502, 1793
CciI TCATGA 2 cut(s) 301, 652
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 3 cut(s) 30, 233, 1463
Cfr13I GGNCC 9 cut(s) 68, 199, 262, 308, 424, 520, 646, 1474, 1689
CseI GACGC 2 cut(s) 556, 1027
Csp6I GTAC 8 cut(s) 824, 1133, 1168, 1392, 1580, 1592, 1950, 2504
CspAI ACCGGT 1 cut(s) 30
CspCI CAANNNNNGTGG 2 cut(s) 1105, 1140
CviQI GTAC 8 cut(s) 824, 1133, 1168, 1392, 1580, 1592, 1950, 2504
DdeI CTNAG 6 cut(s) 178, 203, 874, 2270, 2337, 2532
DraI TTTAAA 1 cut(s) 2001
DraIII CACNNNGTG 1 cut(s) 2245
DrdI GACNNNNNNGTC 1 cut(s) 2407
DseDI GACNNNNNNGTC 1 cut(s) 2407
Eam1104I CTCTTC 5 cut(s) 230, 458, 616, 2114, 2166
EarI CTCTTC 5 cut(s) 230, 458, 616, 2114, 2166
EciI GGCGGA 1 cut(s) 220
Eco105I TACGTA 1 cut(s) 1171
Eco130I CCWWGG 2 cut(s) 1300, 1875
Eco147I AGGCCT 1 cut(s) 2080
Eco31I GGTCTC 1 cut(s) 2327
Eco47I GGWCC 5 cut(s) 68, 262, 520, 646, 1689
Eco57I CTGAAG 7 cut(s) 38, 612, 861, 876, 927, 1770, 2529
Eco91I GGTNACC 1 cut(s) 809
EcoNI CCTNNNNNAGG 1 cut(s) 2165
EcoO109I RGGNCCY 3 cut(s) 262, 520, 646
EcoO65I GGTNACC 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 835
EcoT14I CCWWGG 2 cut(s) 1300, 1875
EcoT22I ATGCAT 1 cut(s) 1838
ErhI CCWWGG 2 cut(s) 1300, 1875
Esp3I CGTCTC 2 cut(s) 12, 85
FalI AAGNNNNNCTT 4 cut(s) 942, 974, 2139, 2171
FaqI GGGAC 6 cut(s) 54, 275, 533, 659, 1030, 2503
FauNDI CATATG 3 cut(s) 685, 1320, 1846
FbaI TGATCA 3 cut(s) 973, 1642, 1849
FblI GTMKAC 1 cut(s) 161
FokI GGATG 9 cut(s) 70, 401, 444, 612, 721, 1138, 1427, 1828, 2252
FspAI RTGCGCAY 1 cut(s) 558
FspBI CTAG 2 cut(s) 444, 2015
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 1 cut(s) 558
GsuI CTGGAG 3 cut(s) 381, 1620, 2134
HaeIII GGCC 6 cut(s) 200, 310, 426, 1463, 1475, 2080
HapII CCGG 6 cut(s) 31, 63, 234, 1188, 1464, 1571
HgaI GACGC 2 cut(s) 556, 1027
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HindIII AAGCTT 2 cut(s) 1971, 2087
HinfI GANTC 7 cut(s) 119, 137, 154, 176, 1280, 1447, 2005
HpaII CCGG 6 cut(s) 31, 63, 234, 1188, 1464, 1571
HphI GGTGA 6 cut(s) 128, 323, 821, 913, 1679, 2398
Hpy166II GTNNAC 2 cut(s) 162, 2389
Hpy8I GTNNAC 2 cut(s) 162, 2389
Hpy99I CGWCG 3 cut(s) 92, 550, 2560
HpyCH4III ACNGT 9 cut(s) 242, 571, 588, 676, 805, 815, 1267, 1812, 1927
HpyCH4IV ACGT 4 cut(s) 1170, 2378, 2492, 2502
HpyF3I CTNAG 6 cut(s) 178, 203, 874, 2270, 2337, 2532
HpySE526I ACGT 4 cut(s) 1170, 2378, 2492, 2502
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 557
Kpn2I TCCGGA 1 cut(s) 1570
Ksp22I TGATCA 3 cut(s) 973, 1642, 1849
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 3 cut(s) 362, 1334, 2518
Lsp1109I GCAGC 8 cut(s) 61, 207, 411, 650, 938, 1926, 2458, 2564
LweI GCATC 4 cut(s) 1160, 1760, 2023, 2451
MaeI CTAG 2 cut(s) 444, 2015
MaeII ACGT 4 cut(s) 1170, 2378, 2492, 2502
MbiI CCGCTC 1 cut(s) 2536
MflI RGATCY 2 cut(s) 1573, 2591
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 4 cut(s) 111, 1066, 1864, 2300
Mph1103I ATGCAT 1 cut(s) 1838
MroI TCCGGA 1 cut(s) 1570
MroXI GAANNNNTTC 4 cut(s) 1101, 1364, 1974, 2196
MslI CAYNNNNRTG 1 cut(s) 1934
MspA1I CMGCKG 2 cut(s) 617, 929
MspCI CTTAAG 1 cut(s) 1595
MspI CCGG 6 cut(s) 31, 63, 234, 1188, 1464, 1571
Mva1269I GAATGC 1 cut(s) 1838
MvnI CGCG 1 cut(s) 194
NdeI CATATG 3 cut(s) 685, 1320, 1846
NlaIV GGNNCC 8 cut(s) 70, 263, 309, 521, 647, 748, 1771, 2593
NmeAIII GCCGAG 1 cut(s) 2142
NmuCI GTSAC 5 cut(s) 236, 329, 809, 964, 1243
NsbI TGCGCA 1 cut(s) 558
NsiI ATGCAT 1 cut(s) 1838
NspI RCATGY 1 cut(s) 515
PagI TCATGA 2 cut(s) 301, 652
PceI AGGCCT 1 cut(s) 2080
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PctI GAATGC 1 cut(s) 1838
PdmI GAANNNNTTC 4 cut(s) 1101, 1364, 1974, 2196
PfeI GAWTC 6 cut(s) 119, 137, 154, 1280, 1447, 2005
Pfl23II CGTACG 1 cut(s) 1167
PinAI ACCGGT 1 cut(s) 30
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Ppu21I YACGTR 2 cut(s) 1171, 2379
PpuMI RGGWCCY 3 cut(s) 262, 520, 646
PshBI ATTAAT 2 cut(s) 1491, 2286
PsiI TTATAA 2 cut(s) 800, 1655
Psp5II RGGWCCY 3 cut(s) 262, 520, 646
PspEI GGTNACC 1 cut(s) 809
PspLI CGTACG 1 cut(s) 1167
PspN4I GGNNCC 8 cut(s) 70, 263, 309, 521, 647, 748, 1771, 2593
PspPI GGNCC 9 cut(s) 68, 199, 262, 308, 424, 520, 646, 1474, 1689
PspPPI RGGWCCY 3 cut(s) 262, 520, 646
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 2 cut(s) 1502, 1793
PsuI RGATCY 2 cut(s) 1573, 2591
PvuII CAGCTG 2 cut(s) 617, 929
RsaI GTAC 8 cut(s) 825, 1134, 1169, 1393, 1581, 1593, 1951, 2505
RsaNI GTAC 8 cut(s) 824, 1133, 1168, 1392, 1580, 1592, 1950, 2504
RseI CAYNNNNRTG 1 cut(s) 1934
SapI GCTCTTC 1 cut(s) 230
Sau96I GGNCC 9 cut(s) 68, 199, 262, 308, 424, 520, 646, 1474, 1689
SchI GAGTC 1 cut(s) 170
SfaNI GCATC 4 cut(s) 1160, 1760, 2023, 2451
SfcI CTRYAG 3 cut(s) 47, 658, 1623
SinI GGWCC 5 cut(s) 68, 262, 520, 646, 1689
SmiMI CAYNNNNRTG 1 cut(s) 1934
SmlI CTYRAG 3 cut(s) 1033, 1397, 1595
SmoI CTYRAG 3 cut(s) 1033, 1397, 1595
SnaBI TACGTA 1 cut(s) 1171
SseBI AGGCCT 1 cut(s) 2080
SspMI CTAG 2 cut(s) 444, 2015
StuI AGGCCT 1 cut(s) 2080
StyI CCWWGG 2 cut(s) 1300, 1875
TaaI ACNGT 9 cut(s) 242, 571, 588, 676, 805, 815, 1267, 1812, 1927
TaiI ACGT 4 cut(s) 1173, 2381, 2495, 2505
TaqI TCGA 4 cut(s) 143, 152, 2200, 2582
TaqII GACCGA 1 cut(s) 147
TatI WGTACW 2 cut(s) 1591, 1949
TauI GCSGC 2 cut(s) 1475, 1536
TfiI GAWTC 6 cut(s) 119, 137, 154, 1280, 1447, 2005
TscAI CASTG 6 cut(s) 591, 1270, 1449, 1555, 1924, 2102
TseFI GTSAC 5 cut(s) 236, 329, 809, 964, 1243
TseI GCWGC 8 cut(s) 49, 220, 399, 663, 926, 1914, 2471, 2552
Tsp45I GTSAC 5 cut(s) 236, 329, 809, 964, 1243
TspDTI ATGAA 8 cut(s) 88, 129, 198, 641, 683, 1091, 1695, 1845
TspGWI ACGGA 3 cut(s) 612, 841, 2581
TspRI CASTG 6 cut(s) 591, 1270, 1449, 1555, 1924, 2102
Vha464I CTTAAG 1 cut(s) 1595
VpaK11BI GGWCC 5 cut(s) 68, 262, 520, 646, 1689
VspI ATTAAT 2 cut(s) 1491, 2286
XagI CCTNNNNNAGG 1 cut(s) 2165
XapI RAATTY 3 cut(s) 789, 835, 1025
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 4 cut(s) 1101, 1364, 1974, 2196
XspI CTAG 2 cut(s) 444, 2015
Zsp2I ATGCAT 1 cut(s) 1838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.