MD02G1009900.v1.1

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009289
Physical Location & Seq
Forward (+)
651086 .. 659187
8102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1009900.v1.1.491

Sequence Viewer

Length: 4131 bp
ATGGAAGACGACGACGAATTCGGAGATCTCTACACCGACGTTCTTCGACCCTTTGAATCGTCCGAACCATCGTTATCATCTGCGCCGCAGCCCCACCAATCCTCCGCCGCGCCCCAATCTTTCAACCGTCCGATCGATCTCAACGTCCTCGACGAAGAGGACAAGATCCTTTTTGCAGCTCCGCACTCTAATCCTTCTGTTTCTCACCCGTCCAATTCCCAAACCCTAGCTCCCGCCGCCTCTGTTCCAACCAATTCCGCTAGAGATGCCGCTCCAGTTGGTGGGTCTAGGGTTTTGGAGGCTAAAGATGTCGAATTGCCGAAAGTCAATTCCGTGGATTTGAACATTGGTGGTAAAGATTTGGATTCGATGGACAAGGATGTGAATTTCGACATCGAGGAGGTTAACAATGGAGCTGATGCTATGGGTTTGGGTCCAGTGATTCCCGGGCTTTCGGATACCTTTCCGGTGAATGATTCCGCAGCGAATTTCGGAAACCCGGAAGTTACAAGAAGAGATGGTGAGAGAGGGGAAGATGACTGGGACAGCGACGATAGCGAGGACGATTTGCAGATAGTATTGAACGATAACAACCACGGGCCTATGGATATGGAGAGAGGTGGAATGGGCGGCGAAGATGACGATGACGATGACGGCCTCGTTATTGTGGCTGATAGTGAGCCGAACCAGCCAATGGACGACCAAGAGTGGGTGGAAGAGAGCGCACAGGCGGCGGAAGGTGAGAGGAAGGAGATGGCTGAAGCCGGGAAGACTGCTGGCGGCGTGGTTGTGCCCCCAAAAGTTGGGTATAGCAGTCATGGGTATCATCCATTTCATTCTCAGTTTAAGTATGTAAGACCTGGGGCTGTGCCAATGCCTGGACCACCTACATCTGGCCTTGGAGGAGTCCCACTCCCAGGTCAAGTTCGTCCACTTGTAAACTTGGGTCCTGGAGCTGGTCGAGGTAGAGGTGACTGGAGACCAACAGGAATGAAAAATGGCACTCCACTGCAGAAGAACTCTCATCCAGGCTTTGGTACACCTGGCTGGAGCAACAATATGGGTGGTCGCGGTTTCGGTGGTGGACTGGATTTCACACTTCCTTCACACAAGACCATATTTGATGTTGTCATTGATGGTTTTGAGGAAAAACCTTGGAAGTATCCTGGTGTTGATACATCGGACTTTTTCAATTTTGGCTTAAATGAAGATAGCTGGAAAGATTATTGCAAACAGCTGGAACAGCTTCGCTTGGAGTCCACCATGCAAAGTAAGATTCGTGTTTATGAAAGTGGACGAGCAGAACAGGAGTATGATCCAGATCTGCCCCCAGAGCTTGCAGCAGCAACTGGTATTCATGATTTTCCTGCTGAAAATGTAAATCCTGGGAAGTCGGATGTTGTCCAAAGTGATTTAGCAAAAGGATCTGCTCGTCTGCGTCCTCCTATACCAACTGGAAGAGCAATTCAGGTGGAAGGTGGTTTTGGTGAGCGTCTCCCCTCGATTGATACCCGACCACCTCGAGTTCGTGATTCAGATGCAATCATTGAGATTGTCTTGCAGGACTCTTTAGATGATGATTCCTCTGCAGGAAATGGTATCCCAGACGGAGCTGAAAATGATCGCCCAAGAGAGGGTTTTGGTAGAGGTGAAGGAGACCTCCCACAGGTTGAGAGCGAGTATTTTGATGGCTCTCCCCAGGCTTATAACGATCAAAAGGTTGGAAGAAAAATGCCTTTCCATGATAACATACCAGAAGAAGGAAACTTACCCTCCCCTCCTGAAGTGGCAGTCCCATATACTGGTTCTGGGGGTGAGACTCCTAGTTATCAGGAAAGGAAAACACAACGAAGAGCATGTGACAGATCTCCTCATGTGACTCCTAGTCGAAATATAGGGGATAAGAAATGCCTTGAAAATCAGAAGGAAGAATCGATTGAAAGCATGGATGGTAAACATAGTCCAGGGATATCATCACCCGTCACAAATAGAGCTGCCCGGGAGTCAAGTGCTGATTATAGAGATAGTGACCAAGATGAGCCTGTTCTGGCTGATGGAAGCTCTGAAATGGGAAAGGAGGAAACATCTACTGTTGCTGAAAATGATGCCTTGCAAGATGGGGCTCCAAAGCATAAACGATTAGTTTCAAGAGTTGAACAGTCTGCTGACGAAGAACTTGATGATGGTGAGGACTCAAAAGCTGCAAAAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGCAGGGATTATCCGAAGTGGAGGGATGGGGTTGAAGAGGAAGTCATTCAAGGACGTTCAACACATATGGGGGGCATCAAGAGGCACCTTGATGAAAATGAAAAGGGGTTCCAGAGGAAAAACCGTGATGGTAGACAGGAACCAGATAGAAGTCACATGGTAGTTAAAGGGAGGGAGGGCTCTTATCCTTATAGGGACTGGGATCCTAGCTCTGCACATCAGTTGCAATTGAAAAATGATGGCCTTCATGGGCGAAAGGAGAGGGACAATCTTGATGTACCGTGGCAACGGAGAGAAAATGATCCTTGTAGCAGAAGGATCAGGCCTGAAGAAACAAGGAAGAGAGAACGTAGTGATGAAATGGGATCTAGGCACAGGAGTAAGGTTCGAGAAGGTGACAGGAACGACAAAGACGAACATCTGCAGTCAAGAAAACAAGTGGACAATGGTAGCTATAGGGTTTATCATGATAAGGATGTCGGTTCACGACCCAGGGAAAGGGAAGGTAGTTTGAAGGCCAGGTATGAACATGTGGAAGATTACCATGGCAAGAGAAGGAAAGATGAGGAATATATGAAGAGAGACCATATAGATAAAGAAGATTTTTTGCATGGGCACAGAGATAATAGCACTCGCAGAAAGCGTGAAAGGGATGAAATTCTGGATCAGCGTAAGAGAGATGAGCAACAAAGAGTAAGAGAAAATCTTGATGATCTGCACCCTGCTAGGCACAAAGATGATGGCTGGTCACATAGGGAGAGAGGTGACAGGCAAAGAGAGAAGGAGGAGTGGCATAGGGTCAAACAATCCCAGGAGGAAAATATACCCAAGCGTGAAAGAGATGAAGGACGAGTTGCTATAAGGGGTGGGCGTGGTGCAGAAGACAAAGCATGGGTAGGCCATACTAGGGCAAAGGATGAGAACAAGGGCGCTGATAAGGAGCACCAATATAAAGAGACTGCAAGGCACAGTGAACCATCTAAAAGGAGGGATAGGGTTGAGGAAGAAAGCTCACATCGTAGGGGACGTGAAGATGTTCATGGACGTGGAAATCAAATAAATAATGATGAGAAAAGATCCGGAAAGGAGAGATCAAGTACTCGTAATGAGCGTGCGGACAGCCAAAAGGTCCATGACAGAAAACATAAAGAAAATTCAAGGAAGAGTAAAGAATCTGAGATTGCTAATAACAGCACCACTTCCAAGAGACATCAAGAAGATCAAAGTGGTCATAATAAGGAGATGGGCTTGAAAGGCACCCGTGTGCAAGGAACTGGTGAGGAAATCCCACCGCAGCGTCACTCTTCCAAAAGGCATAAGGAAGATGTTTCCTCTGATGATGAACAACAGGATTTGAAAAGGGGGCGCTCCAAATTGGAGCGCTGGACAAGCCATAAGGAGAGGGATTTCAGTATCAACAGTAAATCATCTTTGAAGCTCAAAGAGCTTGATAGGATCAACAACCGAGGATCCTCAGATGCCAAGAAAGTTCCAGAGGAACTCAAAGAGCTTGATAGGATCAACAACAGAGGATCCGCAGATGCCAGTAAAGTTCGAGAGGAATCTTCCAAGCCAGTTGAGGCTGTTGATAATCAACATTCAATGGCCGAGGAGAAAGATGCCGGTGATCAGGAGGATATCAAGGATGCAGACACAAAACCATTGGAGGAGCGACACCTGGACACAGTCGAGAAGTTGAAGAAAAGAAGTGAGCGTTTCAAGCGTCCGATGCCAAGTGAAAAAGAGCCAGCGGCAATCAAAAAGGTGGAGAGTGATGTGCCGCCTTCCACCATTAGCGAGACTCAGACTCAGACTCAGACTCAGACTCCTGTGGAGTCAGAGATCAAACCAGAACGGCCAGCTAGGAAAAGAAGGTGGATCAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1377

Amino Acids

154.77

Weight (kDa)

5.46

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 375 - 417 1.1e-19 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1707
AccB1I GGYRCC 2 cut(s) 2325, 3497
AccB7I CCANNNNNTGG 6 cut(s) 281, 694, 803, 878, 1034, 1802
AccBSI CCGCTC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 2374
AccII CGCG 2 cut(s) 110, 1071
AccIII TCCGGA 1 cut(s) 3320
AcoI YGGCCR 2 cut(s) 3846, 4097
AcsI RAATTY 5 cut(s) 17, 385, 487, 2898, 3394
AcuI CTGAAG 3 cut(s) 780, 1803, 2589
AfaI GTAC 3 cut(s) 1039, 2520, 3340
AfeI AGCGCT 1 cut(s) 3623
AflIII ACRYGT 1 cut(s) 2770
AhdI GACNNNNNGTC 1 cut(s) 1884
AjiI CACGTC 2 cut(s) 3269, 3287
AjuI GAANNNNNNNTTGG 2 cut(s) 1467, 1499
AleI CACNNNNGTG 1 cut(s) 3503
Alw21I GWGCWC 1 cut(s) 3186
Alw26I GTCTC 8 cut(s) 973, 1499, 1650, 1811, 2817, 3191, 3442, 4034
AlwNI CAGNNNCTG 2 cut(s) 1349, 4128
Ama87I CYCGRG 3 cut(s) 446, 1521, 1998
Aor13HI TCCGGA 1 cut(s) 3320
Aor51HI AGCGCT 1 cut(s) 3623
AoxI GGCC 9 cut(s) 599, 655, 895, 2482, 2564, 2757, 3139, 3846, 4097
ApeKI GCWGC 9 cut(s) 88, 176, 482, 1340, 1343, 1994, 2201, 2241, 3535
ApoI RAATTY 5 cut(s) 17, 385, 487, 2898, 3394
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 3 cut(s) 1245, 2286, 3276
AspLEI GCGC 6 cut(s) 85, 112, 725, 3173, 3609, 3624
AspS9I GGNCC 5 cut(s) 434, 599, 881, 947, 3370
AsuC2I CCSGG 6 cut(s) 447, 448, 500, 766, 1999, 2000
AvaI CYCGRG 3 cut(s) 446, 1521, 1998
AvaII GGWCC 4 cut(s) 434, 881, 947, 3370
BaeGI GKGCMC 2 cut(s) 795, 2859
BamHI GGATCC 3 cut(s) 2443, 3710, 3773
BanI GGYRCC 2 cut(s) 2325, 3497
BanII GRGCYC 2 cut(s) 2125, 2423
BarI GAAGNNNNNNTAC 2 cut(s) 1752, 1784
BbsI GAAGAC 3 cut(s) 12, 776, 3129
Bbv12I GWGCWC 1 cut(s) 3186
BbvI GCAGC 9 cut(s) 100, 188, 494, 1352, 1355, 1981, 2188, 2253, 3547
BceAI ACGGC 2 cut(s) 670, 4112
BciVI GTATCC 3 cut(s) 451, 1173, 1610
BclI TGATCA 1 cut(s) 3868
BcnI CCSGG 6 cut(s) 447, 448, 500, 766, 1999, 2000
BcoDI GTCTC 8 cut(s) 973, 1499, 1650, 1811, 2817, 3191, 3442, 4034
BfmI CTRYAG 4 cut(s) 1010, 1587, 2663, 2695
BfoI RGCGCY 3 cut(s) 3174, 3610, 3625
BfuI GTATCC 3 cut(s) 451, 1173, 1610
BglII AGATCT 3 cut(s) 25, 1321, 1865
BmcAI AGTACT 1 cut(s) 3340
Bme18I GGWCC 4 cut(s) 434, 881, 947, 3370
BmeRI GACNNNNNGTC 1 cut(s) 1884
BmeT110I CYCGRG 3 cut(s) 446, 1521, 1998
BmgBI CACGTC 2 cut(s) 3269, 3287
BmgT120I GGNCC 5 cut(s) 434, 599, 881, 947, 3370
BmrI ACTGGG 2 cut(s) 550, 2449
BmuI ACTGGG 2 cut(s) 550, 2449
BpiI GAAGAC 3 cut(s) 12, 776, 3129
BplI GAGNNNNNCTC 2 cut(s) 897, 929
BpmI CTGGAG 4 cut(s) 258, 972, 997, 1069
BpuMI CCSGG 6 cut(s) 447, 448, 500, 766, 1999, 2000
Bsa29I ATCGAT 2 cut(s) 135, 1934
BsaBI GATNNNNATC 1 cut(s) 1320
BsaI GGTCTC 3 cut(s) 973, 1650, 2817
BsaWI WCCGGW 2 cut(s) 466, 3320
BsaXI ACNNNNNCTCC 8 cut(s) 86, 116, 214, 244, 1757, 1787, 4051, 4081
Bse118I RCCGGY 1 cut(s) 3863
Bse8I GATNNNNATC 1 cut(s) 1320
BseAI TCCGGA 1 cut(s) 3320
BseCI ATCGAT 2 cut(s) 135, 1934
BseJI GATNNNNATC 1 cut(s) 1320
BseMII CTCAG 7 cut(s) 854, 3408, 3729, 4058, 4064, 4070, 4076
BseRI GAGGAG 6 cut(s) 413, 918, 1860, 3041, 3866, 3923
BseSI GKGCMC 2 cut(s) 795, 2859
BseXI GCAGC 9 cut(s) 100, 188, 494, 1352, 1355, 1981, 2188, 2253, 3547
BsgI GTGCAG 3 cut(s) 2439, 2942, 3138
Bsh1236I CGCG 2 cut(s) 110, 1071
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 9 cut(s) 601, 657, 897, 2484, 2566, 2759, 3141, 3848, 4099
BshNI GGYRCC 2 cut(s) 2325, 3497
BshVI ATCGAT 2 cut(s) 135, 1934
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 3186
BsiHKCI CYCGRG 3 cut(s) 446, 1521, 1998
BsiSI CCGG 7 cut(s) 447, 467, 500, 765, 1999, 3321, 3864
BslFI GGGAC 6 cut(s) 557, 893, 1778, 2450, 2519, 3279
BsmAI GTCTC 8 cut(s) 973, 1499, 1650, 1811, 2817, 3191, 3442, 4034
BsmBI CGTCTC 1 cut(s) 1499
BsmFI GGGAC 6 cut(s) 557, 893, 1778, 2450, 2519, 3279
BsnI GGCC 9 cut(s) 601, 657, 897, 2484, 2566, 2759, 3141, 3848, 4099
Bso31I GGTCTC 3 cut(s) 973, 1650, 2817
BsoBI CYCGRG 3 cut(s) 446, 1521, 1998
Bsp1286I GDGCHC 5 cut(s) 795, 2125, 2423, 2859, 3186
Bsp13I TCCGGA 1 cut(s) 3320
Bsp19I CCATGG 1 cut(s) 2785
BspANI GGCC 9 cut(s) 601, 657, 897, 2484, 2566, 2759, 3141, 3848, 4099
BspCNI CTCAG 7 cut(s) 853, 3409, 3728, 4057, 4063, 4069, 4075
BspDI ATCGAT 2 cut(s) 135, 1934
BspEI TCCGGA 1 cut(s) 3320
BspFNI CGCG 2 cut(s) 110, 1071
BspHI TCATGA 2 cut(s) 1357, 2707
BspMAI CTGCAG 3 cut(s) 1014, 1591, 2667
BspQI GCTCTTC 2 cut(s) 1453, 1846
BspT107I GGYRCC 2 cut(s) 2325, 3497
BspTNI GGTCTC 3 cut(s) 973, 1650, 2817
BsrBI CCGCTC 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 3863
BssAI RCCGGY 1 cut(s) 3863
BssT1I CCWWGG 3 cut(s) 898, 1154, 2785
Bst4CI ACNGT 8 cut(s) 128, 2092, 2160, 2366, 2523, 3212, 3662, 3928
BstC8I GCNNGC 5 cut(s) 778, 1338, 3354, 3990, 4101
BstDEI CTNAG 7 cut(s) 840, 3417, 3715, 4044, 4050, 4056, 4062
BstDSI CCRYGG 4 cut(s) 333, 595, 2522, 2785
BstENI CCTNNNNNAGG 1 cut(s) 1664
BstFNI CGCG 2 cut(s) 110, 1071
BstH2I RGCGCY 3 cut(s) 3174, 3610, 3625
BstHHI GCGC 6 cut(s) 85, 112, 725, 3173, 3609, 3624
BstMAI GTCTC 8 cut(s) 973, 1499, 1650, 1811, 2817, 3191, 3442, 4034
BstMCI CGRYCG 1 cut(s) 135
BstNSI RCATGY 2 cut(s) 1860, 2774
BstSFI CTRYAG 4 cut(s) 1010, 1587, 2663, 2695
BstSLI GKGCMC 2 cut(s) 795, 2859
BstUI CGCG 2 cut(s) 110, 1071
BstV1I GCAGC 9 cut(s) 100, 188, 494, 1352, 1355, 1981, 2188, 2253, 3547
BstV2I GAAGAC 3 cut(s) 12, 776, 3129
Bsu15I ATCGAT 2 cut(s) 135, 1934
BsuI GTATCC 3 cut(s) 451, 1173, 1610
BsuRI GGCC 9 cut(s) 601, 657, 897, 2484, 2566, 2759, 3141, 3848, 4099
BsuTUI ATCGAT 2 cut(s) 135, 1934
BtgI CCRYGG 4 cut(s) 333, 595, 2522, 2785
BtrI CACGTC 2 cut(s) 3269, 3287
BtsI GCAGTG 1 cut(s) 1007
BtsIMutI CAGTG 3 cut(s) 444, 1007, 3217
Cac8I GCNNGC 5 cut(s) 778, 1338, 3354, 3990, 4101
CaiI CAGNNNCTG 2 cut(s) 1349, 4128
CciI TCATGA 2 cut(s) 1357, 2707
CfoI GCGC 6 cut(s) 85, 112, 725, 3173, 3609, 3624
Cfr10I RCCGGY 1 cut(s) 3863
Cfr13I GGNCC 5 cut(s) 434, 599, 881, 947, 3370
Cfr9I CCCGGG 2 cut(s) 446, 1998
ClaI ATCGAT 2 cut(s) 135, 1934
CseI GACGC 4 cut(s) 1427, 1481, 3527, 3953
Csp6I GTAC 3 cut(s) 1038, 2519, 3339
CspCI CAANNNNNGTGG 4 cut(s) 1045, 1080, 1452, 1487
CviQI GTAC 3 cut(s) 1038, 2519, 3339
DdeI CTNAG 7 cut(s) 840, 3417, 3715, 4044, 4050, 4056, 4062
DriI GACNNNNNGTC 1 cut(s) 1884
EaeI YGGCCR 2 cut(s) 3846, 4097
Eam1105I GACNNNNNGTC 1 cut(s) 1884
EciI GGCGGA 2 cut(s) 94, 749
Eco130I CCWWGG 3 cut(s) 898, 1154, 2785
Eco147I AGGCCT 1 cut(s) 2566
Eco24I GRGCYC 2 cut(s) 2125, 2423
Eco31I GGTCTC 3 cut(s) 973, 1650, 2817
Eco32I GATATC 2 cut(s) 1971, 3880
Eco47I GGWCC 4 cut(s) 434, 881, 947, 3370
Eco47III AGCGCT 1 cut(s) 3623
Eco57I CTGAAG 3 cut(s) 780, 1803, 2589
Eco88I CYCGRG 3 cut(s) 446, 1521, 1998
EcoNI CCTNNNNNAGG 1 cut(s) 1664
EcoO109I RGGNCCY 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 17
EcoRV GATATC 2 cut(s) 1971, 3880
EcoT14I CCWWGG 3 cut(s) 898, 1154, 2785
EcoT38I GRGCYC 2 cut(s) 2125, 2423
ErhI CCWWGG 3 cut(s) 898, 1154, 2785
Esp3I CGTCTC 1 cut(s) 1499
FaqI GGGAC 6 cut(s) 557, 893, 1778, 2450, 2519, 3279
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 1 cut(s) 2307
FbaI TGATCA 1 cut(s) 3868
FblI GTMKAC 1 cut(s) 2374
FriOI GRGCYC 2 cut(s) 2125, 2423
GlaI GCGC 6 cut(s) 84, 111, 724, 3172, 3608, 3623
GsuI CTGGAG 4 cut(s) 258, 972, 997, 1069
HaeII RGCGCY 3 cut(s) 3174, 3610, 3625
HaeIII GGCC 9 cut(s) 601, 657, 897, 2484, 2566, 2759, 3141, 3848, 4099
HapII CCGG 7 cut(s) 447, 467, 500, 765, 1999, 3321, 3864
HgaI GACGC 4 cut(s) 1427, 1481, 3527, 3953
HhaI GCGC 6 cut(s) 85, 112, 725, 3173, 3609, 3624
Hin6I GCGC 6 cut(s) 83, 110, 723, 3171, 3607, 3622
HinP1I GCGC 6 cut(s) 83, 110, 723, 3171, 3607, 3622
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 7 cut(s) 447, 467, 500, 765, 1999, 3321, 3864
Hpy99I CGWCG 5 cut(s) 14, 17, 41, 155, 554
HpyCH4III ACNGT 8 cut(s) 128, 2092, 2160, 2366, 2523, 3212, 3662, 3928
HpyCH4IV ACGT 6 cut(s) 39, 144, 2296, 2590, 3268, 3286
HpyF3I CTNAG 7 cut(s) 840, 3417, 3715, 4044, 4050, 4056, 4062
HpySE526I ACGT 6 cut(s) 39, 144, 2296, 2590, 3268, 3286
HspAI GCGC 6 cut(s) 83, 110, 723, 3171, 3607, 3622
Kpn2I TCCGGA 1 cut(s) 3320
Ksp22I TGATCA 1 cut(s) 3868
KspAI GTTAAC 1 cut(s) 406
LguI GCTCTTC 2 cut(s) 1453, 1846
Lsp1109I GCAGC 9 cut(s) 100, 188, 494, 1352, 1355, 1981, 2188, 2253, 3547
MaeII ACGT 6 cut(s) 39, 144, 2296, 2590, 3268, 3286
MbiI CCGCTC 1 cut(s) 272
MfeI CAATTG 1 cut(s) 2468
MhlI GDGCHC 5 cut(s) 795, 2125, 2423, 2859, 3186
MmeI TCCRAC 3 cut(s) 272, 1374, 1702
MroI TCCGGA 1 cut(s) 3320
MroXI GAANNNNTTC 3 cut(s) 1245, 2286, 3276
MseI TTAA 4 cut(s) 405, 846, 1202, 2406
MslI CAYNNNNRTG 4 cut(s) 2331, 2976, 3285, 3503
MspA1I CMGCKG 2 cut(s) 1237, 3992
MspI CCGG 7 cut(s) 447, 467, 500, 765, 1999, 3321, 3864
MunI CAATTG 1 cut(s) 2468
MvnI CGCG 2 cut(s) 110, 1071
NciI CCSGG 6 cut(s) 447, 448, 500, 766, 1999, 2000
NcoI CCATGG 1 cut(s) 2785
NdeI CATATG 1 cut(s) 2307
NmeAIII GCCGAG 1 cut(s) 3874
NspI RCATGY 2 cut(s) 1860, 2774
OliI CACNNNNGTG 1 cut(s) 3503
PaeR7I CTCGAG 1 cut(s) 1521
PagI TCATGA 2 cut(s) 1357, 2707
PasI CCCWGGG 1 cut(s) 2733
PceI AGGCCT 1 cut(s) 2566
PciI ACATGT 1 cut(s) 2770
PciSI GCTCTTC 2 cut(s) 1453, 1846
PcsI WCGNNNNNNNCGW 6 cut(s) 141, 150, 2652, 2881, 3265, 3349
PdmI GAANNNNTTC 3 cut(s) 1245, 2286, 3276
PflFI GACNNNGTC 1 cut(s) 3926
PflMI CCANNNNNTGG 6 cut(s) 281, 694, 803, 878, 1034, 1802
PfoI TCCNGGA 1 cut(s) 949
Ple19I CGATCG 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 947
PscI ACATGT 1 cut(s) 2770
PsiI TTATAA 1 cut(s) 1707
Psp5II RGGWCCY 1 cut(s) 947
PspPI GGNCC 5 cut(s) 434, 599, 881, 947, 3370
PspPPI RGGWCCY 1 cut(s) 947
PspXI VCTCGAGB 1 cut(s) 1521
PstI CTGCAG 3 cut(s) 1014, 1591, 2667
PstNI CAGNNNCTG 2 cut(s) 1349, 4128
PsyI GACNNNGTC 1 cut(s) 3926
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 1237
RsaI GTAC 3 cut(s) 1039, 2520, 3340
RsaNI GTAC 3 cut(s) 1038, 2519, 3339
RseI CAYNNNNRTG 4 cut(s) 2331, 2976, 3285, 3503
SapI GCTCTTC 2 cut(s) 1453, 1846
SaqAI TTAA 4 cut(s) 405, 846, 1202, 2406
Sau96I GGNCC 5 cut(s) 434, 599, 881, 947, 3370
ScaI AGTACT 1 cut(s) 3340
SduI GDGCHC 5 cut(s) 795, 2125, 2423, 2859, 3186
SfcI CTRYAG 4 cut(s) 1010, 1587, 2663, 2695
Sfr274I CTCGAG 1 cut(s) 1521
SinI GGWCC 4 cut(s) 434, 881, 947, 3370
SlaI CTCGAG 1 cut(s) 1521
SmaI CCCGGG 2 cut(s) 448, 2000
SmiMI CAYNNNNRTG 4 cut(s) 2331, 2976, 3285, 3503
SmlI CTYRAG 1 cut(s) 1521
SmoI CTYRAG 1 cut(s) 1521
SseBI AGGCCT 1 cut(s) 2566
StuI AGGCCT 1 cut(s) 2566
StyI CCWWGG 3 cut(s) 898, 1154, 2785
TaaI ACNGT 8 cut(s) 128, 2092, 2160, 2366, 2523, 3212, 3662, 3928
TaiI ACGT 6 cut(s) 42, 147, 2299, 2593, 3271, 3289
TatI WGTACW 1 cut(s) 3338
TauI GCSGC 9 cut(s) 88, 110, 239, 272, 633, 734, 783, 3995, 4024
Tru1I TTAA 4 cut(s) 405, 846, 1202, 2406
Tru9I TTAA 4 cut(s) 405, 846, 1202, 2406
TscAI CASTG 3 cut(s) 444, 1014, 3217
TseI GCWGC 9 cut(s) 88, 176, 482, 1340, 1343, 1994, 2201, 2241, 3535
TspGWI ACGGA 3 cut(s) 322, 1623, 2545
TspMI CCCGGG 2 cut(s) 446, 1998
TspRI CASTG 3 cut(s) 444, 1014, 3217
Tth111I GACNNNGTC 1 cut(s) 3926
Van91I CCANNNNNTGG 6 cut(s) 281, 694, 803, 878, 1034, 1802
VpaK11BI GGWCC 4 cut(s) 434, 881, 947, 3370
XagI CCTNNNNNAGG 1 cut(s) 1664
XapI RAATTY 5 cut(s) 17, 385, 487, 2898, 3394
XceI RCATGY 2 cut(s) 1860, 2774
XhoI CTCGAG 1 cut(s) 1521
XmaI CCCGGG 2 cut(s) 446, 1998
XmiI GTMKAC 1 cut(s) 2374
XmnI GAANNNNTTC 3 cut(s) 1245, 2286, 3276
ZrmI AGTACT 1 cut(s) 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.