Rw2G001240

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009289
Physical Location & Seq
Forward (+)
1038396 .. 1046141
7746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001240.1

Sequence Viewer

Length: 3993 bp
ATGGAAGACGACGATGAATTCGGAGATCTCTACACCGACGTTCTCCAATCCTTCCAGTCATCATCCCAGCAGTCCTCCTCAGCTCCGGCGCCGCCGCAATCGCTCCACCGTCCGATCGATCTCAACCTCAAGACCGAAGACGCCGACGACGAGATCCTAGCCCCAGCTCCTCCTCCTCGCTCCCACGAGTCCAATCCCAATTCGCAAACCCTAGCTCCCGATTCGACCAGAGCCGCCGATTCGGGCGGCGGTGCTAGGGTTTTGGAGGCGAAAGACGTCAAATTGCAGAGTAAGGATTCGGATTTGACGGAAAAGGAGGTGAATTTCGATATCGAGGAGGACGGGAACAATGGAATTTCCGGTCTGGGATTGGACGCCGTGATTCCGGGGCTTTCGGCCGCCGCGCCGGTGAATAACACGGCGGCGAATGTGGTGAACAACCCGGAAGGTTCGAGAAGGGACGGCGGCGGAGGTGGAGGCGATGATTGGGACAGTGATGATAGCGAGGATGATTTGCAGATTGTGTTGAATGATAACAACCCAATGGGGATGGAGAGAGGCAATGGCGAAGAGGATGACGACGACGGGTTGGTTATTGTGGCGGACAGTGAGCACAACGGGCCGGCGGAGGAGCAAGAGTGGGAAGAGGGGCAGCAGGGGGCGGCGGAGGGGGAGAGGAAGGAGATGGGGGAGGCAGGGAAAGGCGGAGGAGGCGGTCCGGTGGTGGCTCCGAAAATTGGGTATAGCAATCATGGGTATCATCCATTTCATTCCCAGTTTAAGTATGTGAGACCTGGTGCTGTGCCAATGCCTGCACCAACAACTTCTGGTCCTGGAGGAGTCCCGGGTCAAGTTCGTCCACTTGTAAACATGGGTCCAACACCTGGTCGAGGCAGAGGTGACTGGAGACCAACTGGATTAAAAAATGGTACCCCGCTGCAGAAAAATTTTCATTCGGGCTTTGGTACACCTGGTTGGGGAAACAATATGGGTGGTCGCGGTTTTGGGGGTGGACTTGAATTCACACTTCCTTCTCACAAGACCATATTTGATGTCGACATTGACGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACTTCAGACTATTTCAACTTTGGATTGAATGAAGATAGCTGGAGAGATTATTGCAAACAGCTGGAACAGCTCCGCTTGGAGTCCACCATGCAAAGCAAAATCCGTGTTTATGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCTCCAGAATTGGCAGCAGCAACTGGTATGCATGACTTTCCTGCTGAAAATTCCAACCCTGGGAAGTCAGAGGGTGGGCAAAGTGATTTTGCAAAAGGATCTGCTCGCCTGCGTCCACCTATTCCAACTGGAAGAGCAATACAGGTGGAAAGTGGCTATGGTGAACGTTTCCCCTCAAGTGAAAACCGGCCACAGCGAATGCGTGACTCAGATGCTGTCATTGAAATTGTCTTGCAGGACTCTTTAGATGATGATTCTTCTGCAGGAAATGGTATCCCAGACGGAAGTGAAAACGATCCTTCAAAGGAGGGTGGAAGTGCTATTGGTGAGGGAGACCTTCCACAGGATGACAAGGCCTACTTCAATGGTTTTCCACAGGCTCACAATAATCGAAAGAGGGATCCACTTGAAAGAAAAAAGCCATTCAATGATAACGTGCCAGAAGATGATGAAAGCTTGCCCTTCAGTCCAGAAGGGCCAGTCCAACATGCTGGTTCTGGGGACCAGACTCCCAGTTCTGCTGGTGGTAGTTTTGGAGAAAATAGGGGGACACAGAGAAGAGCACGTGACAGATCTCCTCGTTTGACTCCTAGCCGGAATACAAGAGACAAGAAATTTCCTGACAGTCAAAAGGAAGGATCAGTGGAAAGCGTGGATGGTAGACGTAGTCCGTTGATCTCATCCCCCGCCTCACATGGGGCTGCCCGCGAGTCAAGTGTCGAGCATAGAGGCGGTGACCAAGATGAGACTCTGCTGGGTGATGAAAACTCTGGAATGGAAAAGGAGGAAATGGCTGTGAATGTAACTGATGATGCCCATGATGGAGTTCCAAACTATCAAAAGTTGAGTTCACGAGTTGACCAGTCTGCTGATGAAGAACTTGATGATGGCGAAGACTCCAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGTAGAGATTATCAGAAGTGGAGGGATGGGGTTGAGGAGGAAGTCATTCAAGGACGTTCAGCTCACTCGGGAGGCATTAAGAGTCACCTTGATGAAAAGGAACATGGATTCCAAAGAAAAGGCCGTGATGGTAGACAGGAACTGGATAGAAGTCACGTGTTAGTCAAAGGGAGGGAGGGTTCTTATCCGTACAGGGATTGGGATCCTAGCTCTGCTCATCATTTGCAATTGAAAAATGATGGGCTTAATAGGCGAAAAGAGAGGGAAAATCTTGACGGAGCATGGCAAAGGAGGGATGATGATCCTTATAGCAGAAGGATTAGAACTGAAGAACCAAGGAAGAGGGAACGTGGTGATGAAATGGGATCCAGGCACAGAAGTAAGGTTCGTGAAAGCGAGAGGAGTGACAAAGATGAATATATGCAGTCAAGAAAGCAGTTAGATAATGGCAGCTATAGGGTCTTTTATGATAAGGATGTTGGATCACGACCCAGGGAAAGAGAAGATAGTTTGAAGGGCAGGTATGAACATATAGATGATTACCATGGCAAGAGAAGGAAAGATGAGGAATACATGAGGAGGGACCAAATAGATAAAGAAGAACTTCTGCAGGGCCACAGAGATACTACCACTCGCCGAAAGCGGGAAAGGGATGAAGTTTTAGATCAGCGTAAAAGAGATGACCAGCAGAAAGTCAGGGATAATCCTGATGACCACCACTCTGTCAGGCACAAAGATGAAAGCTGGTTGCCAAGGGAGAGAGGTGACAGACAAAGAGAAAGGGAGGAGTGGCATAGGCTTAAGCAATCCCATGAAGAAAATATACCCAAGAGGGAAAGAGATGATGGACGAGTTTCTGTAAGGGGTGGGCGTGCTGCAGAAGACAAAGCATGGGTAGGCCATGCTAGGGCAAAGGATGAGAACAAGGGCTCTGATAAGGAGTACCAATATAAAGAGACTGTGCGACACAGTGAGCAGTCTAAAAGAAGGGATAGGGTTGAGGAGGAAAGTTCACATCATAGGGGGCGTGAGGATGCTCATGCACGGGGAAATCAAATGAATATTGATGAGAGGAGATCTGGCAAGGAGAGATCAAGTACTCGCAGTGAACGTGGTGATAGCCAAAAAGTCCAAGATAGAAAACATAAAGAGAATTCAAGAAGGAATAAAGAAACTGAAATTGCTGATATCAACACTTCTGTCACTTCCAAGAGACATCAAGAAGACCAAAGTGGTCGTAGCAAGGAGATGGGCTTGAAAGGCACCCGTGAGCAAGGACCTGGTCACTCTTCTAAAAGGCACAGGGAAGATGGTTCCTCTGATGATGAGCAGCAGGATTTTAAAAAGGGGCGCTCAAAATTGGAACGATGGACAAGTCATAAGGAGAGGGATTTCAGTATCAACAGTAAGGTATCCTCTACCTTAAAGTCCAAAGAGCTTGATAGGGGATCCTCAGATGCCAGTAAACTTCCAGATGATTCCAAGCCAGTTGAGGCTGTTGATAACCATCACCCGTTGGCTGAGGAGAATACTGGTGATCAGGATATTAAGGATGTTGACACAAAACCATTAGATACAGACACGGCATTGGAGGGTCGTCACCTGGACACAGTTGAGAAGTTGAAGAAACGAAGTGAGCGCTTCAAGCATCCATTGCCAAGTGAAAAAGAACCCTCAACAATTAAAAAGACTGAGACTGAAGTGCTGCCAACCCCTAATAGTGACCCTCCTGTGGTGGAAACTGAGATTAAACCAGAACGGCCAGCTCGGAAAAGGAGATGGATCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1330

Amino Acids

149.13

Weight (kDa)

5.4

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 351 - 393 2.5e-21 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 2709
Acc65I GGTACC 1 cut(s) 929
AccB1I GGYRCC 3 cut(s) 88, 929, 3461
AccB7I CCANNNNNTGG 1 cut(s) 884
AccI GTMKAC 3 cut(s) 1056, 1915, 2332
AccII CGCG 3 cut(s) 404, 999, 1962
AclI AACGTT 1 cut(s) 1420
AcoI YGGCCR 3 cut(s) 396, 1442, 3959
AcsI RAATTY 8 cut(s) 17, 322, 354, 946, 1019, 1303, 1868, 3352
AcuI CTGAAG 4 cut(s) 1092, 1702, 2547, 3918
AcvI CACGTG 2 cut(s) 1820, 2356
AcyI GRCGYC 4 cut(s) 89, 141, 276, 375
AfaI GTAC 5 cut(s) 931, 967, 2390, 3143, 3298
AfeI AGCGCT 1 cut(s) 3839
AflII CTTAAG 1 cut(s) 2999
AflIII ACRYGT 1 cut(s) 2355
AloI GAACNNNNNNTCC 4 cut(s) 2292, 2324, 2362, 2394
Alw21I GWGCWC 2 cut(s) 615, 1819
Alw26I GTCTC 8 cut(s) 784, 901, 1581, 1854, 1994, 3149, 3406, 3887
AlwNI CAGNNNCTG 3 cut(s) 1277, 1469, 2341
Ama87I CYCGRG 2 cut(s) 844, 2266
Aor51HI AGCGCT 1 cut(s) 3839
AoxI GGCC 9 cut(s) 396, 620, 1442, 1608, 1730, 2320, 2812, 3097, 3959
ApoI RAATTY 8 cut(s) 17, 322, 354, 946, 1019, 1303, 1868, 3352
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 2244, 2802
Asp718I GGTACC 1 cut(s) 929
AspLEI GCGC 4 cut(s) 91, 406, 3552, 3840
AspS9I GGNCC 9 cut(s) 620, 716, 830, 875, 1730, 1756, 2782, 2812, 3476
AsuC2I CCSGG 4 cut(s) 387, 443, 845, 846
AvaI CYCGRG 2 cut(s) 844, 2266
AvaII GGWCC 6 cut(s) 716, 830, 875, 1756, 2782, 3476
BamHI GGATCC 4 cut(s) 1654, 2401, 2564, 3647
BanI GGYRCC 3 cut(s) 88, 929, 3461
BanII GRGCYC 1 cut(s) 3131
BarI GAAGNNNNNNTAC 2 cut(s) 3006, 3038
BauI CACGAG 2 cut(s) 185, 2106
BbrPI CACGTG 2 cut(s) 1820, 2356
BbsI GAAGAC 5 cut(s) 12, 144, 2154, 3087, 3429
Bbv12I GWGCWC 2 cut(s) 615, 1819
BbvCI CCTCAGC 2 cut(s) 79, 3720
BceAI ACGGC 6 cut(s) 362, 435, 478, 2307, 3798, 3974
BciVI GTATCC 2 cut(s) 1538, 3622
BclI TGATCA 1 cut(s) 3736
BcnI CCSGG 4 cut(s) 387, 443, 845, 846
BcoDI GTCTC 8 cut(s) 784, 901, 1581, 1854, 1994, 3149, 3406, 3887
BfaI CTAG 6 cut(s) 158, 212, 255, 1845, 2406, 3105
BfmI CTRYAG 5 cut(s) 938, 1515, 2653, 2807, 3075
BfoI RGCGCY 3 cut(s) 92, 3553, 3841
BfrI CTTAAG 1 cut(s) 2999
BfuAI ACCTGC 1 cut(s) 2709
BfuI GTATCC 2 cut(s) 1538, 3622
BglII AGATCT 4 cut(s) 25, 1249, 1826, 3275
BmcAI AGTACT 1 cut(s) 3298
Bme18I GGWCC 6 cut(s) 716, 830, 875, 1756, 2782, 3476
BmeT110I CYCGRG 2 cut(s) 844, 2266
BmgT120I GGNCC 9 cut(s) 620, 716, 830, 875, 1730, 1756, 2782, 2812, 3476
BmrI ACTGGG 2 cut(s) 769, 1761
BmsI GCATC 5 cut(s) 1456, 2056, 3223, 3646, 3856
BmuI ACTGGG 2 cut(s) 769, 1761
BpiI GAAGAC 5 cut(s) 12, 144, 2154, 3087, 3429
BpmI CTGGAG 4 cut(s) 855, 925, 1165, 1242
Bpu10I CCTNAGC 2 cut(s) 79, 3720
BpuEI CTTGAG 2 cut(s) 113, 1414
BpuMI CCSGG 4 cut(s) 387, 443, 845, 846
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 2 cut(s) 1820, 2356
BsaBI GATNNNNATC 4 cut(s) 1248, 2400, 2499, 3705
BsaHI GRCGYC 4 cut(s) 89, 141, 276, 375
BsaI GGTCTC 3 cut(s) 784, 901, 1581
BsaWI WCCGGW 2 cut(s) 359, 718
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 623, 653, 2362, 2392
Bse118I RCCGGY 3 cut(s) 406, 622, 1440
Bse3DI GCAATG 2 cut(s) 568, 3851
Bse8I GATNNNNATC 4 cut(s) 1248, 2400, 2499, 3705
BseCI ATCGAT 1 cut(s) 117
BseJI GATNNNNATC 4 cut(s) 1248, 2400, 2499, 3705
BseMI GCAATG 2 cut(s) 568, 3851
BseMII CTCAG 6 cut(s) 93, 1476, 3666, 3711, 3882, 3933
BseX3I CGGCCG 1 cut(s) 396
BseYI CCCAGC 3 cut(s) 66, 163, 2008
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 3 cut(s) 404, 999, 1962
Bsh1285I CGRYCG 2 cut(s) 117, 399
BshFI GGCC 9 cut(s) 398, 622, 1444, 1610, 1732, 2322, 2814, 3099, 3961
BshNI GGYRCC 3 cut(s) 88, 929, 3461
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 117, 399
BsiHKAI GWGCWC 2 cut(s) 615, 1819
BsiHKCI CYCGRG 2 cut(s) 844, 2266
BslFI GGGAC 6 cut(s) 473, 503, 827, 1769, 1816, 2795
BsmAI GTCTC 8 cut(s) 784, 901, 1581, 1854, 1994, 3149, 3406, 3887
BsmFI GGGAC 6 cut(s) 473, 503, 827, 1769, 1816, 2795
BsmI GAATGC 1 cut(s) 1458
BsnI GGCC 9 cut(s) 398, 622, 1444, 1610, 1732, 2322, 2814, 3099, 3961
Bso31I GGTCTC 3 cut(s) 784, 901, 1581
BsoBI CYCGRG 2 cut(s) 844, 2266
Bsp1286I GDGCHC 3 cut(s) 615, 1819, 3131
Bsp19I CCATGG 1 cut(s) 2743
BspANI GGCC 9 cut(s) 398, 622, 1444, 1610, 1732, 2322, 2814, 3099, 3961
BspCNI CTCAG 6 cut(s) 92, 1475, 3665, 3712, 3883, 3934
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 3 cut(s) 404, 999, 1962
BspMAI CTGCAG 4 cut(s) 942, 1519, 2811, 3079
BspMI ACCTGC 1 cut(s) 2709
BspQI GCTCTTC 2 cut(s) 1381, 1807
BspT107I GGYRCC 3 cut(s) 88, 929, 3461
BspTI CTTAAG 1 cut(s) 2999
BspTNI GGTCTC 3 cut(s) 784, 901, 1581
BsrDI GCAATG 2 cut(s) 568, 3851
BsrFI RCCGGY 3 cut(s) 406, 622, 1440
BssAI RCCGGY 3 cut(s) 406, 622, 1440
BssNI GRCGYC 4 cut(s) 89, 141, 276, 375
BssSI CACGAG 2 cut(s) 185, 2106
BssT1I CCWWGG 5 cut(s) 1082, 2154, 2534, 2743, 2951
Bst2BI CACGAG 2 cut(s) 185, 2106
Bst4CI ACNGT 9 cut(s) 110, 494, 608, 1067, 1880, 3160, 3170, 3605, 3811
Bst6I CTCTTC 6 cut(s) 564, 639, 1381, 1807, 2534, 3493
BstACI GRCGYC 4 cut(s) 89, 141, 276, 375
BstAFI CTTAAG 1 cut(s) 2999
BstAPI GCANNNNNTGC 1 cut(s) 3853
BstBAI YACGTR 2 cut(s) 1820, 2356
BstC8I GCNNGC 8 cut(s) 624, 813, 1360, 1364, 1712, 1960, 3072, 3963
BstDEI CTNAG 6 cut(s) 79, 1462, 3652, 3720, 3891, 3942
BstDSI CCRYGG 1 cut(s) 2743
BstEII GGTNACC 1 cut(s) 1988
BstENI CCTNNNNNAGG 2 cut(s) 888, 1595
BstFNI CGCG 3 cut(s) 404, 999, 1962
BstH2I RGCGCY 3 cut(s) 92, 3553, 3841
BstHHI GCGC 4 cut(s) 91, 406, 3552, 3840
BstMAI GTCTC 8 cut(s) 784, 901, 1581, 1854, 1994, 3149, 3406, 3887
BstMCI CGRYCG 2 cut(s) 117, 399
BstMWI GCNNNNNNNGC 8 cut(s) 100, 619, 711, 1171, 2449, 3459, 3844, 3853
BstNSI RCATGY 1 cut(s) 1745
BstPI GGTNACC 1 cut(s) 1988
BstSFI CTRYAG 5 cut(s) 938, 1515, 2653, 2807, 3075
BstUI CGCG 3 cut(s) 404, 999, 1962
BstV2I GAAGAC 5 cut(s) 12, 144, 2154, 3087, 3429
BstXI CCANNNNNNTGG 1 cut(s) 1745
BstZI CGGCCG 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 2 cut(s) 1538, 3622
BsuRI GGCC 9 cut(s) 398, 622, 1444, 1610, 1732, 2322, 2814, 3099, 3961
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 2743
BtgZI GCGATG 1 cut(s) 495
BtsI GCAGTG 1 cut(s) 3310
BtsIMutI CAGTG 5 cut(s) 499, 613, 1902, 3175, 3310
BveI ACCTGC 1 cut(s) 2709
Cac8I GCNNGC 8 cut(s) 624, 813, 1360, 1364, 1712, 1960, 3072, 3963
CaiI CAGNNNCTG 3 cut(s) 1277, 1469, 2341
CfoI GCGC 4 cut(s) 91, 406, 3552, 3840
Cfr10I RCCGGY 3 cut(s) 406, 622, 1440
Cfr13I GGNCC 9 cut(s) 620, 716, 830, 875, 1730, 1756, 2782, 2812, 3476
Cfr9I CCCGGG 1 cut(s) 844
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 716
CseI GACGC 3 cut(s) 149, 383, 1355
CsiI ACCWGGT 4 cut(s) 793, 883, 970, 3478
Csp6I GTAC 5 cut(s) 930, 966, 2389, 3142, 3297
CspCI CAANNNNNGTGG 4 cut(s) 973, 1008, 1380, 1415
CspI CGGWCCG 1 cut(s) 716
CviQI GTAC 5 cut(s) 930, 966, 2389, 3142, 3297
DdeI CTNAG 6 cut(s) 79, 1462, 3652, 3720, 3891, 3942
DinI GGCGCC 1 cut(s) 90
DraI TTTAAA 1 cut(s) 3541
EaeI YGGCCR 3 cut(s) 396, 1442, 3959
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 6 cut(s) 564, 639, 1381, 1807, 2534, 3493
EarI CTCTTC 6 cut(s) 564, 639, 1381, 1807, 2534, 3493
EciI GGCGGA 5 cut(s) 483, 617, 641, 680, 720
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 5 cut(s) 1082, 2154, 2534, 2743, 2951
Eco147I AGGCCT 1 cut(s) 1610
Eco24I GRGCYC 1 cut(s) 3131
Eco31I GGTCTC 3 cut(s) 784, 901, 1581
Eco32I GATATC 2 cut(s) 331, 3388
Eco47I GGWCC 6 cut(s) 716, 830, 875, 1756, 2782, 3476
Eco47III AGCGCT 1 cut(s) 3839
Eco52I CGGCCG 1 cut(s) 396
Eco57I CTGAAG 4 cut(s) 1092, 1702, 2547, 3918
Eco72I CACGTG 2 cut(s) 1820, 2356
Eco88I CYCGRG 2 cut(s) 844, 2266
Eco91I GGTNACC 1 cut(s) 1988
EcoNI CCTNNNNNAGG 2 cut(s) 888, 1595
EcoO109I RGGNCCY 1 cut(s) 3476
EcoO65I GGTNACC 1 cut(s) 1988
EcoRI GAATTC 3 cut(s) 17, 1019, 3352
EcoRV GATATC 2 cut(s) 331, 3388
EcoT14I CCWWGG 5 cut(s) 1082, 2154, 2534, 2743, 2951
EcoT22I ATGCAT 1 cut(s) 1287
EcoT38I GRGCYC 1 cut(s) 3131
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 5 cut(s) 1082, 2154, 2534, 2743, 2951
FalI AAGNNNNNCTT 4 cut(s) 1598, 1630, 2787, 2819
FaqI GGGAC 6 cut(s) 473, 503, 827, 1769, 1816, 2795
FauI CCCGC 4 cut(s) 942, 1948, 1967, 2835
FbaI TGATCA 1 cut(s) 3736
FblI GTMKAC 3 cut(s) 1056, 1915, 2332
FriOI GRGCYC 1 cut(s) 3131
FspBI CTAG 6 cut(s) 158, 212, 255, 1845, 2406, 3105
GlaI GCGC 4 cut(s) 90, 405, 3551, 3839
GsaI CCCAGC 3 cut(s) 70, 167, 2012
GsuI CTGGAG 4 cut(s) 855, 925, 1165, 1242
HaeII RGCGCY 3 cut(s) 92, 3553, 3841
HaeIII GGCC 9 cut(s) 398, 622, 1444, 1610, 1732, 2322, 2814, 3099, 3961
HgaI GACGC 3 cut(s) 149, 383, 1355
HhaI GCGC 4 cut(s) 91, 406, 3552, 3840
Hin1I GRCGYC 4 cut(s) 89, 141, 276, 375
Hin6I GCGC 4 cut(s) 89, 404, 3550, 3838
HinP1I GCGC 4 cut(s) 89, 404, 3550, 3838
HincII GTYRAC 3 cut(s) 1057, 2113, 3757
HindII GTYRAC 3 cut(s) 1057, 2113, 3757
HindIII AAGCTT 1 cut(s) 1708
Hpy99I CGWCG 6 cut(s) 14, 41, 149, 152, 584, 587
HpyCH4III ACNGT 9 cut(s) 110, 494, 608, 1067, 1880, 3160, 3170, 3605, 3811
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 619, 711, 1171, 2449, 3459, 3844, 3853
HpyF3I CTNAG 6 cut(s) 79, 1462, 3652, 3720, 3891, 3942
Hsp92I GRCGYC 4 cut(s) 89, 141, 276, 375
HspAI GCGC 4 cut(s) 89, 404, 3550, 3838
KasI GGCGCC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 933
KroI GCCGGC 1 cut(s) 622
KroNI GCCGGC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 3736
LguI GCTCTTC 2 cut(s) 1381, 1807
LmnI GCTCC 9 cut(s) 88, 108, 172, 185, 220, 631, 733, 1179, 2477
LweI GCATC 5 cut(s) 1456, 2056, 3223, 3646, 3856
MabI ACCWGGT 4 cut(s) 793, 883, 970, 3478
MaeI CTAG 6 cut(s) 158, 212, 255, 1845, 2406, 3105
MfeI CAATTG 1 cut(s) 2426
MhlI GDGCHC 3 cut(s) 615, 1819, 3131
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 902, 1332, 1403, 1762, 2659
Mph1103I ATGCAT 1 cut(s) 1287
MroNI GCCGGC 1 cut(s) 622
MroXI GAANNNNTTC 2 cut(s) 2244, 2802
MslI CAYNNNNRTG 3 cut(s) 2289, 2733, 2934
MspA1I CMGCKG 2 cut(s) 937, 1165
MspCI CTTAAG 1 cut(s) 2999
MunI CAATTG 1 cut(s) 2426
Mva1269I GAATGC 1 cut(s) 1458
MvnI CGCG 3 cut(s) 404, 999, 1962
MwoI GCNNNNNNNGC 8 cut(s) 100, 619, 711, 1171, 2449, 3459, 3844, 3853
NaeI GCCGGC 1 cut(s) 624
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 4 cut(s) 387, 443, 845, 846
NcoI CCATGG 1 cut(s) 2743
NgoMIV GCCGGC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 1287
NspI RCATGY 1 cut(s) 1745
PasI CCCWGGG 2 cut(s) 1313, 2691
PceI AGGCCT 1 cut(s) 1610
PciSI GCTCTTC 2 cut(s) 1381, 1807
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 3307
PctI GAATGC 1 cut(s) 1458
PdiI GCCGGC 1 cut(s) 624
PdmI GAANNNNTTC 2 cut(s) 2244, 2802
PfeI GAWTC 7 cut(s) 221, 239, 296, 382, 1508, 2307, 3677
PflFI GACNNNGTC 2 cut(s) 1920, 3480
PflMI CCANNNNNTGG 1 cut(s) 884
PfoI TCCNGGA 1 cut(s) 832
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 92
PmaCI CACGTG 2 cut(s) 1820, 2356
PmlI CACGTG 2 cut(s) 1820, 2356
Ppu21I YACGTR 2 cut(s) 1820, 2356
PpuMI RGGWCCY 1 cut(s) 3476
Psp1406I AACGTT 1 cut(s) 1420
Psp5II RGGWCCY 1 cut(s) 3476
PspCI CACGTG 2 cut(s) 1820, 2356
PspEI GGTNACC 1 cut(s) 1988
PspFI CCCAGC 3 cut(s) 66, 163, 2008
PspPI GGNCC 9 cut(s) 620, 716, 830, 875, 1730, 1756, 2782, 2812, 3476
PspPPI RGGWCCY 1 cut(s) 3476
PstI CTGCAG 4 cut(s) 942, 1519, 2811, 3079
PstNI CAGNNNCTG 3 cut(s) 1277, 1469, 2341
PsyI GACNNNGTC 2 cut(s) 1920, 3480
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1165
RsaI GTAC 5 cut(s) 931, 967, 2390, 3143, 3298
RsaNI GTAC 5 cut(s) 930, 966, 2389, 3142, 3297
RseI CAYNNNNRTG 3 cut(s) 2289, 2733, 2934
Rsr2I CGGWCCG 1 cut(s) 716
RsrII CGGWCCG 1 cut(s) 716
SalI GTCGAC 1 cut(s) 1055
SapI GCTCTTC 2 cut(s) 1381, 1807
Sau96I GGNCC 9 cut(s) 620, 716, 830, 875, 1730, 1756, 2782, 2812, 3476
ScaI AGTACT 1 cut(s) 3298
SduI GDGCHC 3 cut(s) 615, 1819, 3131
SexAI ACCWGGT 4 cut(s) 793, 883, 970, 3478
SfaNI GCATC 5 cut(s) 1456, 2056, 3223, 3646, 3856
SfcI CTRYAG 5 cut(s) 938, 1515, 2653, 2807, 3075
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 406
SinI GGWCC 6 cut(s) 716, 830, 875, 1756, 2782, 3476
SmaI CCCGGG 1 cut(s) 846
SmiMI CAYNNNNRTG 3 cut(s) 2289, 2733, 2934
SmlI CTYRAG 3 cut(s) 128, 1429, 2999
SmoI CTYRAG 3 cut(s) 128, 1429, 2999
SseBI AGGCCT 1 cut(s) 1610
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 3262
SspMI CTAG 6 cut(s) 158, 212, 255, 1845, 2406, 3105
StuI AGGCCT 1 cut(s) 1610
StyI CCWWGG 5 cut(s) 1082, 2154, 2534, 2743, 2951
TaaI ACNGT 9 cut(s) 110, 494, 608, 1067, 1880, 3160, 3170, 3605, 3811
TaqI TCGA 9 cut(s) 117, 224, 327, 333, 452, 889, 1056, 1645, 1974
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 1 cut(s) 3296
TauI GCSGC 9 cut(s) 94, 97, 236, 249, 401, 404, 425, 468, 665
TfiI GAWTC 7 cut(s) 221, 239, 296, 382, 1508, 2307, 3677
TscAI CASTG 5 cut(s) 499, 613, 1902, 3175, 3310
TspGWI ACGGA 6 cut(s) 323, 1196, 1551, 1914, 2376, 2490
TspMI CCCGGG 1 cut(s) 844
TspRI CASTG 5 cut(s) 499, 613, 1902, 3175, 3310
Tth111I GACNNNGTC 2 cut(s) 1920, 3480
Van91I CCANNNNNTGG 1 cut(s) 884
Vha464I CTTAAG 1 cut(s) 2999
VpaK11BI GGWCC 6 cut(s) 716, 830, 875, 1756, 2782, 3476
XagI CCTNNNNNAGG 2 cut(s) 888, 1595
XapI RAATTY 8 cut(s) 17, 322, 354, 946, 1019, 1303, 1868, 3352
XceI RCATGY 1 cut(s) 1745
XmaI CCCGGG 1 cut(s) 844
XmiI GTMKAC 3 cut(s) 1056, 1915, 2332
XmnI GAANNNNTTC 2 cut(s) 2244, 2802
XspI CTAG 6 cut(s) 158, 212, 255, 1845, 2406, 3105
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 3298
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.