Rroxscaffold_2G00154880

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009289
Physical Location & Seq
Reverse (-)
91343436 .. 91351359
7924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154880.1

Sequence Viewer

Length: 3987 bp
ATGGAAGACGACGATGAATTCGGAGATCTCTACACCGACGTTCTCCAATCCTTCCAGTCATCATCCCAGCCGTCCTCGTCAGCTCCGGCGCCGCCGCAATCGCTGCACCGTCCGATCGATCTCAACCTCAAGACCGAAGACGCCGACGACGAGATCCTAGCCCCACCTCCTCCTCCTCGCTCCCACGAGTCCAAGCCCAATTCGCAAACCCTAGCTCCCGATTCGACCAGAGCCGCCGATTCGGGCGGCGGTTCTAGGGTTTTGGAGGTGAAAGACGTCAAATTGCAGAGTAAAGATTCGGATTTGACGGAAAAGGAGGTGAATTTCGATATCGAGGAGGAGGGGAACAATGGAATTTCCGGTCTGGGATTGGACGCCGTGATTCCGGGGCTTTCGGCCGCCGCGCCGGTGAATAACACGGCGGCGAATGTGGAGAACAACCCGGAAGGTTCGAGAAGGGACGGCGGCGGAGGCGATGATTGGGACAGTGATGATAGCGAGGATGATTTGCAGATTGTGTTGAATGATAACAACCCAATGGGGATGGAGAGAGGCAATGGCGAAGAGGATGACGACGACGGGTTGGTTATTGTGGCGGACAGTGAGCACAACGGGCCGGCGGAGGAGCAAGAGTGGGAAGAGGGGCAGCAGGGGGCGGCGGAGGGGGAGAGGAAGGAGACGGGGGAGGCAGGGAAAGGCGGAGGAGGCGGTCCGGTGGTGGCTCCGAAAATTGGGTATAGCAATCATGGGTATCATCCATTTCATTCCCAGTTTAAGTATGTGAGACCTGGTGCTGTGCCAATGCCTGGACCAACAACTTCTGGTCCTGGAGGAGTCCCGGGTCAAGTTCGTCCACTTGTAAACATGGGTCCAACACCTGGTCGAGGTAGAGGTGACTGGAGACCAACTGGATTAAAAAATGGTACTCCGCTGCAGAAAAATTTTCATTCGGGCTTTGGTACACCTGGTTGGGGAAACAATATGGGTGGTCGCGGTTTCGGGGGTGGACTTGAATTCACACTTCCTTCTCACAAGACCATATTTGATGTCGACATTGATGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACTTCAGACTATTTCAACTTTGGATTGAATGAAGATAGCTGGAGAGATTATTGCAAACAGCTGGAACAGCTCCGCTTGGAGTCCACCATGCAAAGCAAAATCCGTGTTTATGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCTCCAGAATTGGCAGCAGCAACTGGTATGCATGACTTTCCTGCTGAAAATTCCAACCCTGGGAAGTCAGAGGGTGGGCAGAGTGATTTTGCAAAAGGATCTGCTCGCCTGCGTCCACCTATTCCAACTGGAAGAGCAATACAGGTGGAAAGTGGTTATGGTGAACGTTTCCCCTCAAGTGAAAACCGGCCACAGCGAATGCGTGACTCAGATGCTGTCATTGAAATTGTCTTGCAGGACTCTTTAGATGATGATTCTTCTGCAGGAAATGGTATCCCAGACGGAAGTGAAAACGATCCTTCAAAGGAGGGTGGAAGTGCTATTGGTGAGGGAGACCTTCCACAGGATGACAAGGCCTACTTCAATGGTTTTCCACAGGCTCACAATAATCGAAAGAGGGATCCACTGGAAAGAAAAAAGCCATTCAATGATAACGTGCCAGAAGATGATGAAAGCTTGCCCTTCCGTCCGGAAGGACCAGTCCAACATGCTGGTTCTGGGGACCAGACTCCCAGTTCTGCTGGTGGTAGTTTTGGAGAAAATAGGGGGACACAGAGAAGAGCACGTGACAGATCTCCTCGTTTGACTCCTAGCCGGAATACAAGAGACAAGAAATTTCCTGACAATCAAAAGGAAGGATCAGTGGAAAGCGTGGATGGTAGACGTAGTCCGTTGATCTCATCTCCCGTCTCACATGGGGCTGCCCACGAGTCAAGTGTCGAGCATAGAGGCGGTGACCAAGATGAGACTCTGCTGGGTGATGAAAGCTCTGGAATGGAAAAGGAGGAAATGGCTGCGAATGTAACTGATGATGTCCATGATGGAGTTCCAAACTATCAAAAGTTGAGTTCACGAGTTGACCAGTCTGCTGATGAAGAACTTGATGATGGCGAAGACTCCAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGTAGAGATTATCAGAAGTGGAGGGATGGGGTTGAGGAGGAAGTCATTCAAGGACGTTCAGCTCACTCGGGAGGCATCAAGAGTCACCTTGATGAAAAGGAACAGGGATTCCAAAGAAAAGGCCGTGATGGTAGACAGGAACTGGATAGAAGTCACATGTTAGTCAAAGGGAGGGAGGGTTCTTATCCGTACAGGGATTGGGATCCTAGCTCTGCTCATCATTTGCAATTGAAAAATGATGGGCTTAATAGGCGAAAAGAGAGGGAAAATCTTGACGGAGCATGGCAAAGGAGGGATGATGATCCTTATAGCAGAAGGATTAGAACTGAAGAACCAAGGAAGAGGGAACGTGGTGATGAAATGGGATCCAGGCACAGGAGTAAGGTTCGTGAAAGCGAGAGGAGTGACAAAGATGAATATATGCAGTCAAGAAAGCAGTTAGATAATGGCAGCTATAGAGTCTCTTATGATAAGGATGTTGGATCACGACCCAGGGAAAGAGAAGATAGTTTGAAGGGCAGGTATGAACATATAGATGATTACCATGGCAAGAGAAGGAAAGATGAGGAATACATGAGGAGGGACCAAATAGATAAAGAAGAACTTCTGCAGGGCCACAGAGATACTACCACTCGCCGAAAGCGGGAAAGGGATGAAGTTTTAGATCAGCGTAAAAGAGATGACCAGCAGAAAGTCAGGGATAATCCTGATGACCACCACTCTGTCAGGCACAAAGATGAAAGCTGGTTGCCGAGGGAGAGAGGTGACAGACAAAGAGAAAGGGAGGAGTGGCATAGGCTTAAGCAATCCCATGAAGAAAATATACCCAAGAGGGAAAGAGATGATGGACGAGTTTCTGTAAGGGGTGGGCGTGCTGCAGAAGACAAAGCATGGGTAGGCCATGCTAGGGCAAAGGATGAGAACAAAGGCTCTGATAAGGAGTACCAATATAAAGAGACTGTGCGACACAGTGAGCAGTCTAAAAGAAGGGATAGGGTTGAGGAGGAGAGTTCACATCATAGGGGACGTGAGGATACTCATGCACGGGGAAATCAAATGAATATTGATGAGAGGAGATCTGGCAAGGAGAGATCAAGTACTCGCAGTGAACGTGGTGATAGCCAAAAAGCCCAAGATAGAAAACATAAAGAGAATTCAAGAAGGAATAAAGAAACTGAAATTGCTGATATCAACACTTCAGTCACTTCCAAGAGACATCAAGAAGACCAAAGTGGTCGTAGCAAGGAGATGGGCTTGAAAGGCACCCGTGAGCAAGGACCTGGTCACTCTTCTAAAAGGCACAGGGAAGATGCTTCCTCTGATGATGAGCAGCAGGATTTAAAAAAGGGGCGCTCCAAATTGGAACGATGGACAAGTCATAAGGAGAGGGATTTCAGCATCAACAGTAAGGTATCCTCTACCTTAAAGTTCAAAGAGCTTGATAGGGCATCCTCAGATGCCAGTAAACTTCCAGATGATTCCAAGCCAGTTGAGGCTGTTGATAACCATCACCCATTGGCCGAGGAGAATGCTGGTGATCAGGATATTAAGGATGCTGACACAAAACCATTAGATACAGACACGGCATTGGAGGGTCGTCACCTGGACACAGTTGAGAAGTTGAAGAAACGAAGTGAGCGCTTCAAGCATCCATTGCCAAGTGAAAAAGAACCATCAACAATTAAAAAGACTGATACTGAAGTGCTGCCAACCCCTAATAGTGACCCTCCTGTGGTGGAATCTGAGATTAAACCAGAACGGCCAGCTCGGAAAAGAAGATGGATCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1328

Amino Acids

149.08

Weight (kDa)

5.4

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 349 - 391 2.5e-21 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 2703
AccB1I GGYRCC 2 cut(s) 88, 3455
AccB7I CCANNNNNTGG 2 cut(s) 806, 878
AccI GTMKAC 3 cut(s) 1050, 1909, 2326
AccII CGCG 2 cut(s) 404, 993
AccIII TCCGGA 1 cut(s) 1717
AclI AACGTT 1 cut(s) 1414
AcoI YGGCCR 4 cut(s) 396, 1436, 3711, 3953
AcsI RAATTY 8 cut(s) 17, 322, 354, 940, 1013, 1297, 1862, 3346
AcuI CTGAAG 4 cut(s) 1086, 2541, 3375, 3912
AcvI CACGTG 1 cut(s) 1814
AcyI GRCGYC 4 cut(s) 89, 141, 276, 375
AfaI GTAC 5 cut(s) 925, 961, 2384, 3137, 3292
AfeI AGCGCT 1 cut(s) 3833
AflII CTTAAG 1 cut(s) 2993
AflIII ACRYGT 1 cut(s) 2349
AjiI CACGTC 1 cut(s) 3221
AloI GAACNNNNNNTCC 4 cut(s) 2286, 2318, 2356, 2388
Alw21I GWGCWC 2 cut(s) 609, 1813
AlwNI CAGNNNCTG 3 cut(s) 1271, 1463, 2335
Ama87I CYCGRG 2 cut(s) 838, 2260
Aor13HI TCCGGA 1 cut(s) 1717
Aor51HI AGCGCT 1 cut(s) 3833
AoxI GGCC 9 cut(s) 396, 614, 1436, 1602, 2314, 2806, 3091, 3711, 3953
ApoI RAATTY 8 cut(s) 17, 322, 354, 940, 1013, 1297, 1862, 3346
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 2238, 2796
AspLEI GCGC 4 cut(s) 91, 406, 3546, 3834
AsuC2I CCSGG 4 cut(s) 387, 443, 839, 840
AvaI CYCGRG 2 cut(s) 838, 2260
AvaII GGWCC 8 cut(s) 710, 809, 824, 869, 1724, 1750, 2776, 3470
BaeI ACNNNNGTAYC 2 cut(s) 3879, 3912
BamHI GGATCC 3 cut(s) 1648, 2395, 2558
BanI GGYRCC 2 cut(s) 88, 3455
BarI GAAGNNNNNNTAC 2 cut(s) 3000, 3032
BauI CACGAG 3 cut(s) 185, 1955, 2100
BbrPI CACGTG 1 cut(s) 1814
BbsI GAAGAC 5 cut(s) 12, 144, 2148, 3081, 3423
Bbv12I GWGCWC 2 cut(s) 609, 1813
BceAI ACGGC 7 cut(s) 55, 362, 435, 478, 2301, 3792, 3968
BcgI CGANNNNNNTGC 2 cut(s) 3704, 3738
BciVI GTATCC 3 cut(s) 1532, 3220, 3616
BclI TGATCA 1 cut(s) 3730
BcnI CCSGG 4 cut(s) 387, 443, 839, 840
BfaI CTAG 6 cut(s) 158, 212, 255, 1839, 2400, 3099
BfmI CTRYAG 5 cut(s) 932, 1509, 2647, 2801, 3069
BfoI RGCGCY 3 cut(s) 92, 3547, 3835
BfrI CTTAAG 1 cut(s) 2993
BfuAI ACCTGC 1 cut(s) 2703
BfuI GTATCC 3 cut(s) 1532, 3220, 3616
BglII AGATCT 4 cut(s) 25, 1243, 1820, 3269
BmcAI AGTACT 1 cut(s) 3292
Bme18I GGWCC 8 cut(s) 710, 809, 824, 869, 1724, 1750, 2776, 3470
BmeT110I CYCGRG 2 cut(s) 838, 2260
BmgBI CACGTC 1 cut(s) 3221
BmiI GGNNCC 9 cut(s) 90, 723, 870, 1650, 1751, 2397, 2560, 2777, 3457
BmrI ACTGGG 2 cut(s) 763, 1755
BmsI GCATC 8 cut(s) 1450, 2277, 3493, 3600, 3640, 3650, 3736, 3850
BmuI ACTGGG 2 cut(s) 763, 1755
BpiI GAAGAC 5 cut(s) 12, 144, 2148, 3081, 3423
BpmI CTGGAG 4 cut(s) 849, 919, 1159, 1236
BpuEI CTTGAG 2 cut(s) 113, 1408
BpuMI CCSGG 4 cut(s) 387, 443, 839, 840
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 1814
BsaBI GATNNNNATC 4 cut(s) 1242, 2394, 2493, 3699
BsaHI GRCGYC 4 cut(s) 89, 141, 276, 375
BsaI GGTCTC 3 cut(s) 778, 895, 1575
BsaWI WCCGGW 3 cut(s) 359, 712, 1717
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 617, 647, 2356, 2386
Bse118I RCCGGY 3 cut(s) 406, 616, 1434
Bse3DI GCAATG 2 cut(s) 562, 3845
Bse8I GATNNNNATC 4 cut(s) 1242, 2394, 2493, 3699
BseAI TCCGGA 1 cut(s) 1717
BseCI ATCGAT 1 cut(s) 117
BseJI GATNNNNATC 4 cut(s) 1242, 2394, 2493, 3699
BseMI GCAATG 2 cut(s) 562, 3845
BseMII CTCAG 3 cut(s) 1470, 3660, 3927
BseX3I CGGCCG 1 cut(s) 396
BseYI CCCAGC 2 cut(s) 66, 2002
BsgI GTGCAG 1 cut(s) 89
Bsh1236I CGCG 2 cut(s) 404, 993
Bsh1285I CGRYCG 2 cut(s) 117, 399
BshFI GGCC 9 cut(s) 398, 616, 1438, 1604, 2316, 2808, 3093, 3713, 3955
BshNI GGYRCC 2 cut(s) 88, 3455
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 117, 399
BsiHKAI GWGCWC 2 cut(s) 609, 1813
BsiHKCI CYCGRG 2 cut(s) 838, 2260
BslFI GGGAC 7 cut(s) 473, 497, 821, 1763, 1810, 2789, 3231
BsmBI CGTCTC 2 cut(s) 671, 1942
BsmFI GGGAC 7 cut(s) 473, 497, 821, 1763, 1810, 2789, 3231
BsmI GAATGC 2 cut(s) 1452, 3727
BsnI GGCC 9 cut(s) 398, 616, 1438, 1604, 2316, 2808, 3093, 3713, 3955
Bso31I GGTCTC 3 cut(s) 778, 895, 1575
BsoBI CYCGRG 2 cut(s) 838, 2260
Bsp1286I GDGCHC 2 cut(s) 609, 1813
Bsp13I TCCGGA 1 cut(s) 1717
Bsp19I CCATGG 1 cut(s) 2737
BspANI GGCC 9 cut(s) 398, 616, 1438, 1604, 2316, 2808, 3093, 3713, 3955
BspCNI CTCAG 3 cut(s) 1469, 3659, 3928
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 1717
BspFNI CGCG 2 cut(s) 404, 993
BspLI GGNNCC 9 cut(s) 90, 723, 870, 1650, 1751, 2397, 2560, 2777, 3457
BspMAI CTGCAG 4 cut(s) 936, 1513, 2805, 3073
BspMI ACCTGC 1 cut(s) 2703
BspQI GCTCTTC 2 cut(s) 1375, 1801
BspT107I GGYRCC 2 cut(s) 88, 3455
BspTI CTTAAG 1 cut(s) 2993
BspTNI GGTCTC 3 cut(s) 778, 895, 1575
BsrDI GCAATG 2 cut(s) 562, 3845
BsrFI RCCGGY 3 cut(s) 406, 616, 1434
BssAI RCCGGY 3 cut(s) 406, 616, 1434
BssNI GRCGYC 4 cut(s) 89, 141, 276, 375
BssSI CACGAG 3 cut(s) 185, 1955, 2100
BssT1I CCWWGG 4 cut(s) 1076, 2148, 2528, 2737
Bst2BI CACGAG 3 cut(s) 185, 1955, 2100
Bst4CI ACNGT 7 cut(s) 110, 488, 602, 3154, 3164, 3599, 3805
Bst6I CTCTTC 6 cut(s) 558, 633, 1375, 1801, 2528, 3487
BstACI GRCGYC 4 cut(s) 89, 141, 276, 375
BstAFI CTTAAG 1 cut(s) 2993
BstAPI GCANNNNNTGC 2 cut(s) 103, 3847
BstBAI YACGTR 1 cut(s) 1814
BstC8I GCNNGC 6 cut(s) 618, 1354, 1358, 1706, 3066, 3957
BstDEI CTNAG 3 cut(s) 1456, 3646, 3936
BstDSI CCRYGG 1 cut(s) 2737
BstEII GGTNACC 1 cut(s) 1982
BstENI CCTNNNNNAGG 2 cut(s) 882, 1589
BstFNI CGCG 2 cut(s) 404, 993
BstH2I RGCGCY 3 cut(s) 92, 3547, 3835
BstHHI GCGC 4 cut(s) 91, 406, 3546, 3834
BstMCI CGRYCG 2 cut(s) 117, 399
BstNSI RCATGY 2 cut(s) 1739, 2353
BstPI GGTNACC 1 cut(s) 1982
BstSFI CTRYAG 5 cut(s) 932, 1509, 2647, 2801, 3069
BstUI CGCG 2 cut(s) 404, 993
BstV2I GAAGAC 5 cut(s) 12, 144, 2148, 3081, 3423
BstX2I RGATCY 9 cut(s) 25, 153, 1243, 1346, 1648, 1820, 2395, 2558, 3269
BstXI CCANNNNNNTGG 1 cut(s) 1739
BstYI RGATCY 9 cut(s) 25, 153, 1243, 1346, 1648, 1820, 2395, 2558, 3269
BstZI CGGCCG 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 3 cut(s) 1532, 3220, 3616
BsuRI GGCC 9 cut(s) 398, 616, 1438, 1604, 2316, 2808, 3093, 3713, 3955
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 2737
BtgZI GCGATG 1 cut(s) 489
BtrI CACGTC 1 cut(s) 3221
BtsI GCAGTG 1 cut(s) 3304
BtsIMutI CAGTG 6 cut(s) 493, 607, 1652, 1896, 3169, 3304
BveI ACCTGC 1 cut(s) 2703
Cac8I GCNNGC 6 cut(s) 618, 1354, 1358, 1706, 3066, 3957
CaiI CAGNNNCTG 3 cut(s) 1271, 1463, 2335
CfoI GCGC 4 cut(s) 91, 406, 3546, 3834
Cfr10I RCCGGY 3 cut(s) 406, 616, 1434
Cfr9I CCCGGG 1 cut(s) 838
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 710
CseI GACGC 3 cut(s) 149, 383, 1349
CsiI ACCWGGT 4 cut(s) 787, 877, 964, 3472
Csp6I GTAC 5 cut(s) 924, 960, 2383, 3136, 3291
CspCI CAANNNNNGTGG 4 cut(s) 967, 1002, 1374, 1409
CspI CGGWCCG 1 cut(s) 710
CviQI GTAC 5 cut(s) 924, 960, 2383, 3136, 3291
DdeI CTNAG 3 cut(s) 1456, 3646, 3936
DinI GGCGCC 1 cut(s) 90
DraI TTTAAA 1 cut(s) 3534
EaeI YGGCCR 4 cut(s) 396, 1436, 3711, 3953
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 6 cut(s) 558, 633, 1375, 1801, 2528, 3487
EarI CTCTTC 6 cut(s) 558, 633, 1375, 1801, 2528, 3487
EciI GGCGGA 5 cut(s) 483, 611, 635, 674, 714
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 4 cut(s) 1076, 2148, 2528, 2737
Eco147I AGGCCT 1 cut(s) 1604
Eco31I GGTCTC 3 cut(s) 778, 895, 1575
Eco32I GATATC 2 cut(s) 331, 3382
Eco47I GGWCC 8 cut(s) 710, 809, 824, 869, 1724, 1750, 2776, 3470
Eco47III AGCGCT 1 cut(s) 3833
Eco52I CGGCCG 1 cut(s) 396
Eco57I CTGAAG 4 cut(s) 1086, 2541, 3375, 3912
Eco72I CACGTG 1 cut(s) 1814
Eco88I CYCGRG 2 cut(s) 838, 2260
Eco91I GGTNACC 1 cut(s) 1982
EcoNI CCTNNNNNAGG 2 cut(s) 882, 1589
EcoO109I RGGNCCY 1 cut(s) 3470
EcoO65I GGTNACC 1 cut(s) 1982
EcoRI GAATTC 3 cut(s) 17, 1013, 3346
EcoRV GATATC 2 cut(s) 331, 3382
EcoT14I CCWWGG 4 cut(s) 1076, 2148, 2528, 2737
EcoT22I ATGCAT 1 cut(s) 1281
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 4 cut(s) 1076, 2148, 2528, 2737
Esp3I CGTCTC 2 cut(s) 671, 1942
FalI AAGNNNNNCTT 4 cut(s) 1592, 1624, 2781, 2813
FaqI GGGAC 7 cut(s) 473, 497, 821, 1763, 1810, 2789, 3231
FauI CCCGC 1 cut(s) 2829
FbaI TGATCA 1 cut(s) 3730
FblI GTMKAC 3 cut(s) 1050, 1909, 2326
FspBI CTAG 6 cut(s) 158, 212, 255, 1839, 2400, 3099
GlaI GCGC 4 cut(s) 90, 405, 3545, 3833
GsaI CCCAGC 2 cut(s) 70, 2006
GsuI CTGGAG 4 cut(s) 849, 919, 1159, 1236
HaeII RGCGCY 3 cut(s) 92, 3547, 3835
HaeIII GGCC 9 cut(s) 398, 616, 1438, 1604, 2316, 2808, 3093, 3713, 3955
HgaI GACGC 3 cut(s) 149, 383, 1349
HhaI GCGC 4 cut(s) 91, 406, 3546, 3834
Hin1I GRCGYC 4 cut(s) 89, 141, 276, 375
Hin6I GCGC 4 cut(s) 89, 404, 3544, 3832
HinP1I GCGC 4 cut(s) 89, 404, 3544, 3832
HincII GTYRAC 2 cut(s) 1051, 2107
HindII GTYRAC 2 cut(s) 1051, 2107
HindIII AAGCTT 1 cut(s) 1702
Hpy99I CGWCG 6 cut(s) 14, 41, 149, 152, 578, 581
HpyCH4III ACNGT 7 cut(s) 110, 488, 602, 3154, 3164, 3599, 3805
HpyF3I CTNAG 3 cut(s) 1456, 3646, 3936
Hsp92I GRCGYC 4 cut(s) 89, 141, 276, 375
HspAI GCGC 4 cut(s) 89, 404, 3544, 3832
KasI GGCGCC 1 cut(s) 88
Kpn2I TCCGGA 1 cut(s) 1717
KroI GCCGGC 1 cut(s) 616
KroNI GCCGGC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 3730
LguI GCTCTTC 2 cut(s) 1375, 1801
LmnI GCTCC 8 cut(s) 88, 185, 220, 625, 727, 1173, 2471, 3551
LweI GCATC 8 cut(s) 1450, 2277, 3493, 3600, 3640, 3650, 3736, 3850
MabI ACCWGGT 4 cut(s) 787, 877, 964, 3472
MaeI CTAG 6 cut(s) 158, 212, 255, 1839, 2400, 3099
MfeI CAATTG 1 cut(s) 2420
MflI RGATCY 9 cut(s) 25, 153, 1243, 1346, 1648, 1820, 2395, 2558, 3269
MhlI GDGCHC 2 cut(s) 609, 1813
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 896, 1326, 1397, 1756, 2653
Mph1103I ATGCAT 1 cut(s) 1281
MroI TCCGGA 1 cut(s) 1717
MroNI GCCGGC 1 cut(s) 616
MroXI GAANNNNTTC 2 cut(s) 2238, 2796
MseI TTAA 9 cut(s) 774, 914, 2439, 2994, 3533, 3617, 3741, 3876, 3942
MslI CAYNNNNRTG 3 cut(s) 2283, 2727, 2928
MspA1I CMGCKG 2 cut(s) 931, 1159
MspCI CTTAAG 1 cut(s) 2993
MunI CAATTG 1 cut(s) 2420
Mva1269I GAATGC 2 cut(s) 1452, 3727
MvnI CGCG 2 cut(s) 404, 993
NaeI GCCGGC 1 cut(s) 618
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 4 cut(s) 387, 443, 839, 840
NcoI CCATGG 1 cut(s) 2737
NgoMIV GCCGGC 1 cut(s) 616
NlaIV GGNNCC 9 cut(s) 90, 723, 870, 1650, 1751, 2397, 2560, 2777, 3457
NmeAIII GCCGAG 2 cut(s) 2970, 3739
NsiI ATGCAT 1 cut(s) 1281
NspI RCATGY 2 cut(s) 1739, 2353
PasI CCCWGGG 2 cut(s) 1307, 2685
PceI AGGCCT 1 cut(s) 1604
PciI ACATGT 1 cut(s) 2349
PciSI GCTCTTC 2 cut(s) 1375, 1801
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 3301
PctI GAATGC 2 cut(s) 1452, 3727
PdiI GCCGGC 1 cut(s) 618
PdmI GAANNNNTTC 2 cut(s) 2238, 2796
PfeI GAWTC 8 cut(s) 221, 239, 296, 382, 1502, 2301, 3671, 3932
PflFI GACNNNGTC 2 cut(s) 1914, 3474
PflMI CCANNNNNTGG 2 cut(s) 806, 878
PfoI TCCNGGA 1 cut(s) 826
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 92
PmaCI CACGTG 1 cut(s) 1814
PmlI CACGTG 1 cut(s) 1814
Ppu21I YACGTR 1 cut(s) 1814
PpuMI RGGWCCY 1 cut(s) 3470
PscI ACATGT 1 cut(s) 2349
Psp1406I AACGTT 1 cut(s) 1414
Psp5II RGGWCCY 1 cut(s) 3470
PspCI CACGTG 1 cut(s) 1814
PspEI GGTNACC 1 cut(s) 1982
PspFI CCCAGC 2 cut(s) 66, 2002
PspN4I GGNNCC 9 cut(s) 90, 723, 870, 1650, 1751, 2397, 2560, 2777, 3457
PspPPI RGGWCCY 1 cut(s) 3470
PstI CTGCAG 4 cut(s) 936, 1513, 2805, 3073
PstNI CAGNNNCTG 3 cut(s) 1271, 1463, 2335
PsuI RGATCY 9 cut(s) 25, 153, 1243, 1346, 1648, 1820, 2395, 2558, 3269
PsyI GACNNNGTC 2 cut(s) 1914, 3474
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1159
RsaI GTAC 5 cut(s) 925, 961, 2384, 3137, 3292
RsaNI GTAC 5 cut(s) 924, 960, 2383, 3136, 3291
RseI CAYNNNNRTG 3 cut(s) 2283, 2727, 2928
Rsr2I CGGWCCG 1 cut(s) 710
RsrII CGGWCCG 1 cut(s) 710
SalI GTCGAC 1 cut(s) 1049
SapI GCTCTTC 2 cut(s) 1375, 1801
SaqAI TTAA 9 cut(s) 774, 914, 2439, 2994, 3533, 3617, 3741, 3876, 3942
ScaI AGTACT 1 cut(s) 3292
SduI GDGCHC 2 cut(s) 609, 1813
SexAI ACCWGGT 4 cut(s) 787, 877, 964, 3472
SfaNI GCATC 8 cut(s) 1450, 2277, 3493, 3600, 3640, 3650, 3736, 3850
SfcI CTRYAG 5 cut(s) 932, 1509, 2647, 2801, 3069
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 406
SinI GGWCC 8 cut(s) 710, 809, 824, 869, 1724, 1750, 2776, 3470
SmaI CCCGGG 1 cut(s) 840
SmiMI CAYNNNNRTG 3 cut(s) 2283, 2727, 2928
SmlI CTYRAG 3 cut(s) 128, 1423, 2993
SmoI CTYRAG 3 cut(s) 128, 1423, 2993
SseBI AGGCCT 1 cut(s) 1604
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 3256
SspMI CTAG 6 cut(s) 158, 212, 255, 1839, 2400, 3099
StuI AGGCCT 1 cut(s) 1604
StyI CCWWGG 4 cut(s) 1076, 2148, 2528, 2737
TaaI ACNGT 7 cut(s) 110, 488, 602, 3154, 3164, 3599, 3805
TaqI TCGA 9 cut(s) 117, 224, 327, 333, 452, 883, 1050, 1639, 1968
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 1 cut(s) 3290
TauI GCSGC 9 cut(s) 94, 97, 236, 249, 401, 404, 425, 468, 659
TfiI GAWTC 8 cut(s) 221, 239, 296, 382, 1502, 2301, 3671, 3932
Tru1I TTAA 9 cut(s) 774, 914, 2439, 2994, 3533, 3617, 3741, 3876, 3942
Tru9I TTAA 9 cut(s) 774, 914, 2439, 2994, 3533, 3617, 3741, 3876, 3942
TscAI CASTG 6 cut(s) 493, 607, 1659, 1896, 3169, 3304
TspGWI ACGGA 7 cut(s) 323, 1190, 1545, 1703, 1908, 2370, 2484
TspMI CCCGGG 1 cut(s) 838
TspRI CASTG 6 cut(s) 493, 607, 1659, 1896, 3169, 3304
Tth111I GACNNNGTC 2 cut(s) 1914, 3474
Van91I CCANNNNNTGG 2 cut(s) 806, 878
Vha464I CTTAAG 1 cut(s) 2993
VpaK11BI GGWCC 8 cut(s) 710, 809, 824, 869, 1724, 1750, 2776, 3470
XagI CCTNNNNNAGG 2 cut(s) 882, 1589
XapI RAATTY 8 cut(s) 17, 322, 354, 940, 1013, 1297, 1862, 3346
XceI RCATGY 2 cut(s) 1739, 2353
XmaI CCCGGG 1 cut(s) 838
XmiI GTMKAC 3 cut(s) 1050, 1909, 2326
XmnI GAANNNNTTC 2 cut(s) 2238, 2796
XspI CTAG 6 cut(s) 158, 212, 255, 1839, 2400, 3099
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 3292
Zsp2I ATGCAT 1 cut(s) 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.