Rh2DG016300

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009289
Physical Location & Seq
Forward (+)
936890 .. 944832
7943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG016300.1

Sequence Viewer

Length: 3492 bp
ATGGAAGACGACGATGAATTCGGAGATCTCTACACCGACGTTCTCCAATCCTTCCAGTCATCATCCCAGCAGTCCTCCTCAGCTCCGGCGCCGCCGCAATCGCTCCACCGTCCGATCGATCTCAACCTCAAGACCGAAGACGCCGACGACGAGATCCTAGCCCCAGCTCCTCCTCCTCGCTCCCACGAGTCCAATCCCAATTCGCAAACCCTAGCTCCCGATTCGACCAGAGCCGCCGATTCGGGCGGCGGTGCTAGGGTTTTGGAGGCGAAAGACGTCAAATTGCATAGTAAAGATTCGGATTTGACGGAAAAGGAGGTGAATTTCGATATCGAGGAGGAGGGGAACAATGGGATTTCCGGTCTGGGATTCGACGCCGTGATTCCGGGGCTTTCGGCCGCCGCGCCGGTGAACAACACGGCGGCGAATGTGGAGAACAACCCGGAAGGTTCGAGAAGGGACGGCGGCGGAGGCGGAGGCGGAGGCGATGATTGGGACAGTGATGATAGCGAGGATGATTTGCAGATTGTGTTGAATGATAACAACCCAATGGGGATGGAGAGAGGCAATGGCGAAGAGGATGACGACGACGGGTTGGTTATTGTGGCGGACAGTGAGCACAACGGGCCGGCGGAGGAGCAAGAGTGGGAAGAGGGGCAGCAGGGGGCGGCGGAGGGGGAGAGGAAGGAGATGGGGGAGGCAGGGAAAGGCGGAGGAGGCGGTCCGGTGGTGGCTCCGAAAATTGGGTATAGCAATCATGGGTATCATCCATTTCATTCCCAGTTTAAGTATGTGAGACCTGGTGCTGTGCCAATGCCTGCACCAACAACTTCTGGTCCTGGAGGAGTCCCGGGTCAAGTTCGTCCACTTGTAAACATGGGTCCAACACCTGGTCGAGGTAGAGGTGACTGGAGACCAACTGGATTAAAAAATGGTACCCCGCTGCAGAAAAATTTTCATTCGGGCTTTGGTACACCTGGTTGGGGAAACAATTTGGGTGGTCGCGGTTTTGGGGGTGGACTTGAATTCACACTTCCTTCTCACAAGACCATATTTGATGTCGACATTGACGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACTTCAGACTATTTCAACTTTGGATTGAATGAAGATAGCTGGAGAGATTATTGCAAACAGCTGGAACAGCTCCGCTTGGAGTCCACCATGCAAAGCAAAATCCGTGTTTATGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCTCCAGAATTGGCAGCAGCAACTGGTATGCATGACTTTCCTGCTGAAAATTCCAACCCTGGGAAGTCAGAGGGTGGGCAAAGTGATTTTGCAAAAGGATCTGCTCGCCTGCGTCCACCTATTCCAACTGGAAGAGCAATACAGGTGGAAAGTGGCTATGGTGAACGTTTCCCCTCAAGTGAAAACCGGCCACAGCGAATGCGTGACTCAGATGCTGTCATTGAAATTGTCTTGCAGGACTCTTTAGATGATGATTCTTCTGCAGGAAATGGTATCCCAGACGGAAGTGAAAACGATCCTTCAAAGGAGGGTGGAAGTGCTATTGGTGAGGGAGACCTTCCACAGGATGACAAGGCCTACTTCAATGGTTTTCCACAGGCTCACAATAATCGAAAGAGGGATCCACTTGAAAGAAAAAAGCCATTCAATGATAACGTGCCAGAAGATGATGAAAGCTTGCCCTTCAGTCCAGAAGGGCCAGTCCAACATGCTGGTTCTGGGGACCAGACTCCCAGTTCTGCTGGTGGTAGTTTTGGAGAAAATAGGGGGACACAGAGAAGAGCACGTGACAGATCTCCTCGTTTGACTCCTAGCCGGAATACAAGAGACAAGAAATTTCCTGACAGTCAAAAGGAAGGATCAGTGGAAAGCGTGGATGGTAGACGTAGTCCGTTGATCTCATCCCCCGCCTCACATGGGGCTGCCCGCGAGTCAAGTGTCGAGCATAGAGGCGGTGACCAAGATGAGACTCTGCTGGGTGATGAAAACTCTGGAATGGAAAAGGAGGAAATGGCTGTGAATGTAACTGATGATGTCCATGATGGAGTTCCAAACTATCAAAAGTTGAGTTCACGAGTTGACCAGTCTGCTGATGAAGAACTTGATGATGGCGAAGACTCCAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGTAGAGATTATCAGAAGTGGAGGGATGGGGTTGAGGAGGAAGTCATTCAAGGACGTTCAGCTCACTCGGGAGGCATTAAGAGTCACCTTGATGAAAAGGAACAGGGATTCCAAAGAAAAGGCCGTGATGGTAGACAGGAACTGGATAGAAGTCACATGTTAGTCAAAGGGAGGGAGGGTTCTTATCCGTACAGGGATTGGGATCCTAGCTCTGCTCATCATTTGCAATTGAAAAATGATGGGCTTAATAGGCGAAAAGAGAGGGAAAATCTTGACGGAGCATGGCAAAGGAGGGATGATGATCCTTATAGCAGAAGGATTAGAACTGAAGAACCAAGGAAGAGGGAACGTGGTGATGAAATGGGATCCAGGCACAGAAGTAAGGTTCGTGAAAGCGAGAGGAGTGACAAAGATGAATATATGCAGTCAAGAAAGCAGTTAGATAATGGCAGCTATAGGGTCTTTTATGATAAGGATGTTGGATCACGACCCAGGGAAAGAGAAGATAGTTTGAAGGGCAGGTATGAACATATAGATGATTACCATGGCAAGAGAAGGAAAGATGAGGAATACATGAGGAGGGACCAAATAGATAAAGAAGAACTTCTGCAGGGCCACAGAGATACTACCACTCGCCGAAAGCGGGAAAGGGATGAAGTTTTAGATCAGCGTAAAAGAGATGACCAGCAGAAAGTCAGGGATAATCCTGATGATCACCACTCTGTCAGGCACAAAGATGAAAGCTGGTTGCCGAGGGAGAGAGGTGACAGACAAAGAGAAAGGGAGGAGTGGCATAGGCTTAAGCAATCCCATGAAGAAAATATACCCAAGAGGGAAAGAGATGATGGACGAGTTTCTGTAAGGGGTGGGCGTGCTGCAGAAGACAAAGCATGGGTAGGCCATGCTAGGGCAAAGGATGAGAACAAGGGCTCTGATAAGGAGTACCAATATAAAGAGACTGTGCGACACAGTGAGCAGTCTAAAAGAAGGGATAGGGTTGAGGAGGAAAGTTCACATCATAGGGGGCGTGAGGATGCTCATGCACGGGGAAATCAAATGAATATTGATGAGAGGAGATCTGGCAAGGAGAGATCAAGTACTCGCAGTGAACGTGGTGATAGCCAAAAAGTCCAAGATAGAAAACATAAAGAGAATTCAAGAAGGAATAAAGAAACTGAAATTGCTGATATCAACACTTCTGTCACTTCCAAGAGACATCAAGAAGACCAAAGTGGTCGTAGCAAGGAGATGGTATGTACTGTTCTTGACATCAGGTCTCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1163

Amino Acids

130.19

Weight (kDa)

5.27

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 353 - 395 2.2e-21 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 2715
Acc65I GGTACC 1 cut(s) 935
AccB1I GGYRCC 2 cut(s) 88, 935
AccB7I CCANNNNNTGG 1 cut(s) 890
AccI GTMKAC 3 cut(s) 1062, 1921, 2338
AccII CGCG 3 cut(s) 404, 1005, 1968
AclI AACGTT 1 cut(s) 1426
AcoI YGGCCR 2 cut(s) 396, 1448
AcsI RAATTY 7 cut(s) 17, 322, 952, 1025, 1309, 1874, 3358
AcuI CTGAAG 3 cut(s) 1098, 1708, 2553
AcvI CACGTG 1 cut(s) 1826
AcyI GRCGYC 4 cut(s) 89, 141, 276, 375
AfaI GTAC 6 cut(s) 937, 973, 2396, 3149, 3304, 3463
AfiI CCNNNNNNNGG 9 cut(s) 743, 890, 896, 983, 1320, 1603, 1956, 2848, 3112
AflII CTTAAG 1 cut(s) 3005
AflIII ACRYGT 1 cut(s) 2361
AhdI GACNNNNNGTC 1 cut(s) 3478
AjnI CCWGG 8 cut(s) 799, 838, 889, 976, 1099, 1318, 2573, 2696
AloI GAACNNNNNNTCC 4 cut(s) 2298, 2330, 2368, 2400
Alw21I GWGCWC 2 cut(s) 621, 1825
Alw26I GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3486
AlwNI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
Ama87I CYCGRG 2 cut(s) 850, 2272
AoxI GGCC 8 cut(s) 396, 626, 1448, 1614, 1736, 2326, 2818, 3103
ApeKI GCWGC 8 cut(s) 658, 943, 1274, 1277, 1961, 2165, 2655, 3080
ApoI RAATTY 7 cut(s) 17, 322, 952, 1025, 1309, 1874, 3358
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 2250, 2808
Asp718I GGTACC 1 cut(s) 935
AspLEI GCGC 2 cut(s) 91, 406
AspS9I GGNCC 8 cut(s) 626, 722, 836, 881, 1736, 1762, 2788, 2818
AsuC2I CCSGG 4 cut(s) 387, 443, 851, 852
AvaI CYCGRG 2 cut(s) 850, 2272
AvaII GGWCC 5 cut(s) 722, 836, 881, 1762, 2788
BamHI GGATCC 3 cut(s) 1660, 2407, 2570
BanI GGYRCC 2 cut(s) 88, 935
BanII GRGCYC 1 cut(s) 3137
BarI GAAGNNNNNNTAC 2 cut(s) 3012, 3044
BauI CACGAG 3 cut(s) 185, 2112, 3483
BbrPI CACGTG 1 cut(s) 1826
BbsI GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
Bbv12I GWGCWC 2 cut(s) 621, 1825
BbvCI CCTCAGC 1 cut(s) 79
BbvI GCAGC 8 cut(s) 670, 930, 1286, 1289, 1948, 2152, 2667, 3067
BceAI ACGGC 4 cut(s) 362, 435, 478, 2313
BciT130I CCWGG 8 cut(s) 801, 840, 891, 978, 1101, 1320, 2575, 2698
BciVI GTATCC 1 cut(s) 1544
BclI TGATCA 1 cut(s) 2917
BcnI CCSGG 4 cut(s) 387, 443, 851, 852
BcoDI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3486
BfaI CTAG 6 cut(s) 158, 212, 255, 1851, 2412, 3111
BfmI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
BfoI RGCGCY 1 cut(s) 92
BfrI CTTAAG 1 cut(s) 3005
BfuAI ACCTGC 1 cut(s) 2715
BfuI GTATCC 1 cut(s) 1544
BglII AGATCT 4 cut(s) 25, 1255, 1832, 3281
BmcAI AGTACT 1 cut(s) 3304
Bme18I GGWCC 5 cut(s) 722, 836, 881, 1762, 2788
BmeRI GACNNNNNGTC 1 cut(s) 3478
BmeT110I CYCGRG 2 cut(s) 850, 2272
BmgT120I GGNCC 8 cut(s) 626, 722, 836, 881, 1736, 1762, 2788, 2818
BmiI GGNNCC 9 cut(s) 90, 735, 882, 937, 1662, 1763, 2409, 2572, 2789
BmrI ACTGGG 2 cut(s) 775, 1767
BmsI GCATC 2 cut(s) 1462, 3229
BmuI ACTGGG 2 cut(s) 775, 1767
BpiI GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
BpmI CTGGAG 4 cut(s) 861, 931, 1171, 1248
Bpu10I CCTNAGC 1 cut(s) 79
BpuEI CTTGAG 2 cut(s) 113, 1420
BpuMI CCSGG 4 cut(s) 387, 443, 851, 852
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 1826
BsaBI GATNNNNATC 3 cut(s) 1254, 2406, 2505
BsaHI GRCGYC 4 cut(s) 89, 141, 276, 375
BsaI GGTCTC 4 cut(s) 790, 907, 1587, 3486
BsaWI WCCGGW 2 cut(s) 359, 724
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 629, 659, 2368, 2398
Bsc4I CCNNNNNNNGG 9 cut(s) 743, 890, 896, 983, 1320, 1603, 1956, 2848, 3112
Bse118I RCCGGY 3 cut(s) 406, 628, 1446
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 3 cut(s) 1254, 2406, 2505
BseBI CCWGG 8 cut(s) 801, 840, 891, 978, 1101, 1320, 2575, 2698
BseCI ATCGAT 1 cut(s) 117
BseJI GATNNNNATC 3 cut(s) 1254, 2406, 2505
BseLI CCNNNNNNNGG 9 cut(s) 743, 890, 896, 983, 1320, 1603, 1956, 2848, 3112
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 93, 1482
BseX3I CGGCCG 1 cut(s) 396
BseXI GCAGC 8 cut(s) 670, 930, 1286, 1289, 1948, 2152, 2667, 3067
BseYI CCCAGC 3 cut(s) 66, 163, 2014
BsgI GTGCAG 1 cut(s) 804
Bsh1236I CGCG 3 cut(s) 404, 1005, 1968
Bsh1285I CGRYCG 2 cut(s) 117, 399
BshFI GGCC 8 cut(s) 398, 628, 1450, 1616, 1738, 2328, 2820, 3105
BshNI GGYRCC 2 cut(s) 88, 935
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 117, 399
BsiHKAI GWGCWC 2 cut(s) 621, 1825
BsiHKCI CYCGRG 2 cut(s) 850, 2272
BslFI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
BslI CCNNNNNNNGG 9 cut(s) 743, 890, 896, 983, 1320, 1603, 1956, 2848, 3112
BsmAI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3486
BsmFI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
BsmI GAATGC 1 cut(s) 1464
BsnI GGCC 8 cut(s) 398, 628, 1450, 1616, 1738, 2328, 2820, 3105
Bso31I GGTCTC 4 cut(s) 790, 907, 1587, 3486
BsoBI CYCGRG 2 cut(s) 850, 2272
Bsp1286I GDGCHC 3 cut(s) 621, 1825, 3137
Bsp19I CCATGG 1 cut(s) 2749
BspANI GGCC 8 cut(s) 398, 628, 1450, 1616, 1738, 2328, 2820, 3105
BspCNI CTCAG 2 cut(s) 92, 1481
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 3 cut(s) 404, 1005, 1968
BspLI GGNNCC 9 cut(s) 90, 735, 882, 937, 1662, 1763, 2409, 2572, 2789
BspMAI CTGCAG 4 cut(s) 948, 1525, 2817, 3085
BspMI ACCTGC 1 cut(s) 2715
BspQI GCTCTTC 2 cut(s) 1387, 1813
BspT107I GGYRCC 2 cut(s) 88, 935
BspTI CTTAAG 1 cut(s) 3005
BspTNI GGTCTC 4 cut(s) 790, 907, 1587, 3486
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 3 cut(s) 406, 628, 1446
BssAI RCCGGY 3 cut(s) 406, 628, 1446
BssNI GRCGYC 4 cut(s) 89, 141, 276, 375
BssSI CACGAG 3 cut(s) 185, 2112, 3483
BssT1I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
Bst2BI CACGAG 3 cut(s) 185, 2112, 3483
Bst2UI CCWGG 8 cut(s) 801, 840, 891, 978, 1101, 1320, 2575, 2698
Bst4CI ACNGT 8 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3466
Bst6I CTCTTC 5 cut(s) 570, 645, 1387, 1813, 2540
BstACI GRCGYC 4 cut(s) 89, 141, 276, 375
BstAFI CTTAAG 1 cut(s) 3005
BstBAI YACGTR 1 cut(s) 1826
BstC8I GCNNGC 7 cut(s) 630, 819, 1366, 1370, 1718, 1966, 3078
BstDEI CTNAG 2 cut(s) 79, 1468
BstDSI CCRYGG 1 cut(s) 2749
BstEII GGTNACC 1 cut(s) 1994
BstENI CCTNNNNNAGG 2 cut(s) 894, 1601
BstFNI CGCG 3 cut(s) 404, 1005, 1968
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 91, 406
BstMAI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3486
BstMCI CGRYCG 2 cut(s) 117, 399
BstMWI GCNNNNNNNGC 6 cut(s) 100, 471, 625, 717, 1177, 2455
BstNI CCWGG 8 cut(s) 801, 840, 891, 978, 1101, 1320, 2575, 2698
BstNSI RCATGY 2 cut(s) 1751, 2365
BstPI GGTNACC 1 cut(s) 1994
BstSFI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
BstUI CGCG 3 cut(s) 404, 1005, 1968
BstV1I GCAGC 8 cut(s) 670, 930, 1286, 1289, 1948, 2152, 2667, 3067
BstV2I GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
BstX2I RGATCY 9 cut(s) 25, 153, 1255, 1358, 1660, 1832, 2407, 2570, 3281
BstXI CCANNNNNNTGG 1 cut(s) 1751
BstYI RGATCY 9 cut(s) 25, 153, 1255, 1358, 1660, 1832, 2407, 2570, 3281
BstZI CGGCCG 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 1 cut(s) 1544
BsuRI GGCC 8 cut(s) 398, 628, 1450, 1616, 1738, 2328, 2820, 3105
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 2749
BtgZI GCGATG 1 cut(s) 501
BtsI GCAGTG 1 cut(s) 3316
BtsIMutI CAGTG 5 cut(s) 505, 619, 1908, 3181, 3316
BveI ACCTGC 1 cut(s) 2715
Cac8I GCNNGC 7 cut(s) 630, 819, 1366, 1370, 1718, 1966, 3078
CaiI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
CfoI GCGC 2 cut(s) 91, 406
Cfr10I RCCGGY 3 cut(s) 406, 628, 1446
Cfr13I GGNCC 8 cut(s) 626, 722, 836, 881, 1736, 1762, 2788, 2818
Cfr9I CCCGGG 1 cut(s) 850
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 722
CseI GACGC 3 cut(s) 149, 383, 1361
CsiI ACCWGGT 3 cut(s) 799, 889, 976
Csp6I GTAC 6 cut(s) 936, 972, 2395, 3148, 3303, 3462
CspCI CAANNNNNGTGG 4 cut(s) 979, 1014, 1386, 1421
CspI CGGWCCG 1 cut(s) 722
CviQI GTAC 6 cut(s) 936, 972, 2395, 3148, 3303, 3462
DdeI CTNAG 2 cut(s) 79, 1468
DinI GGCGCC 1 cut(s) 90
DriI GACNNNNNGTC 1 cut(s) 3478
EaeI YGGCCR 2 cut(s) 396, 1448
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 5 cut(s) 570, 645, 1387, 1813, 2540
Eam1105I GACNNNNNGTC 1 cut(s) 3478
EarI CTCTTC 5 cut(s) 570, 645, 1387, 1813, 2540
EciI GGCGGA 7 cut(s) 483, 489, 495, 623, 647, 686, 726
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
Eco147I AGGCCT 1 cut(s) 1616
Eco24I GRGCYC 1 cut(s) 3137
Eco31I GGTCTC 4 cut(s) 790, 907, 1587, 3486
Eco32I GATATC 2 cut(s) 331, 3394
Eco47I GGWCC 5 cut(s) 722, 836, 881, 1762, 2788
Eco52I CGGCCG 1 cut(s) 396
Eco57I CTGAAG 3 cut(s) 1098, 1708, 2553
Eco72I CACGTG 1 cut(s) 1826
Eco88I CYCGRG 2 cut(s) 850, 2272
Eco91I GGTNACC 1 cut(s) 1994
EcoNI CCTNNNNNAGG 2 cut(s) 894, 1601
EcoO65I GGTNACC 1 cut(s) 1994
EcoRI GAATTC 3 cut(s) 17, 1025, 3358
EcoRII CCWGG 8 cut(s) 799, 838, 889, 976, 1099, 1318, 2573, 2696
EcoRV GATATC 2 cut(s) 331, 3394
EcoT14I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
EcoT22I ATGCAT 1 cut(s) 1293
EcoT38I GRGCYC 1 cut(s) 3137
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
FalI AAGNNNNNCTT 4 cut(s) 1604, 1636, 2793, 2825
FaqI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
FauI CCCGC 4 cut(s) 948, 1954, 1973, 2841
FbaI TGATCA 1 cut(s) 2917
FblI GTMKAC 3 cut(s) 1062, 1921, 2338
FriOI GRGCYC 1 cut(s) 3137
FspBI CTAG 6 cut(s) 158, 212, 255, 1851, 2412, 3111
GlaI GCGC 2 cut(s) 90, 405
GsaI CCCAGC 3 cut(s) 70, 167, 2018
GsuI CTGGAG 4 cut(s) 861, 931, 1171, 1248
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 8 cut(s) 398, 628, 1450, 1616, 1738, 2328, 2820, 3105
HgaI GACGC 3 cut(s) 149, 383, 1361
HhaI GCGC 2 cut(s) 91, 406
Hin1I GRCGYC 4 cut(s) 89, 141, 276, 375
Hin6I GCGC 2 cut(s) 89, 404
HinP1I GCGC 2 cut(s) 89, 404
HincII GTYRAC 2 cut(s) 1063, 2119
HindII GTYRAC 2 cut(s) 1063, 2119
HindIII AAGCTT 1 cut(s) 1714
Hpy188I TCNGA 9 cut(s) 23, 114, 301, 738, 1117, 1330, 1471, 2220, 3139
Hpy99I CGWCG 7 cut(s) 14, 41, 149, 152, 377, 590, 593
HpyCH4III ACNGT 8 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3466
HpyCH4IV ACGT 9 cut(s) 39, 276, 1426, 1695, 1825, 1924, 2260, 2554, 3316
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 471, 625, 717, 1177, 2455
HpyF3I CTNAG 2 cut(s) 79, 1468
HpySE526I ACGT 9 cut(s) 39, 276, 1426, 1695, 1825, 1924, 2260, 2554, 3316
Hsp92I GRCGYC 4 cut(s) 89, 141, 276, 375
HspAI GCGC 2 cut(s) 89, 404
KasI GGCGCC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 939
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 1 cut(s) 2917
LguI GCTCTTC 2 cut(s) 1387, 1813
LmnI GCTCC 9 cut(s) 88, 108, 172, 185, 220, 637, 739, 1185, 2483
Lsp1109I GCAGC 8 cut(s) 670, 930, 1286, 1289, 1948, 2152, 2667, 3067
LweI GCATC 2 cut(s) 1462, 3229
MabI ACCWGGT 3 cut(s) 799, 889, 976
MaeI CTAG 6 cut(s) 158, 212, 255, 1851, 2412, 3111
MaeII ACGT 9 cut(s) 39, 276, 1426, 1695, 1825, 1924, 2260, 2554, 3316
MfeI CAATTG 1 cut(s) 2432
MflI RGATCY 9 cut(s) 25, 153, 1255, 1358, 1660, 1832, 2407, 2570, 3281
MhlI GDGCHC 3 cut(s) 621, 1825, 3137
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 908, 1338, 1409, 1768, 2665
Mph1103I ATGCAT 1 cut(s) 1293
MroNI GCCGGC 1 cut(s) 628
MroXI GAANNNNTTC 2 cut(s) 2250, 2808
MseI TTAA 5 cut(s) 786, 926, 2283, 2451, 3006
MslI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
MspA1I CMGCKG 2 cut(s) 943, 1171
MspCI CTTAAG 1 cut(s) 3005
MunI CAATTG 1 cut(s) 2432
Mva1269I GAATGC 1 cut(s) 1464
MvaI CCWGG 8 cut(s) 801, 840, 891, 978, 1101, 1320, 2575, 2698
MvnI CGCG 3 cut(s) 404, 1005, 1968
MwoI GCNNNNNNNGC 6 cut(s) 100, 471, 625, 717, 1177, 2455
NaeI GCCGGC 1 cut(s) 630
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 4 cut(s) 387, 443, 851, 852
NcoI CCATGG 1 cut(s) 2749
NgoMIV GCCGGC 1 cut(s) 628
NlaIV GGNNCC 9 cut(s) 90, 735, 882, 937, 1662, 1763, 2409, 2572, 2789
NmeAIII GCCGAG 1 cut(s) 2982
NsiI ATGCAT 1 cut(s) 1293
NspI RCATGY 2 cut(s) 1751, 2365
PasI CCCWGGG 2 cut(s) 1319, 2697
PceI AGGCCT 1 cut(s) 1616
PciI ACATGT 1 cut(s) 2361
PciSI GCTCTTC 2 cut(s) 1387, 1813
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 3313
PctI GAATGC 1 cut(s) 1464
PdiI GCCGGC 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 2250, 2808
PfeI GAWTC 7 cut(s) 221, 239, 296, 369, 382, 1514, 2313
PflFI GACNNNGTC 1 cut(s) 1926
PflMI CCANNNNNTGG 1 cut(s) 890
PfoI TCCNGGA 1 cut(s) 838
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 92
PmaCI CACGTG 1 cut(s) 1826
PmlI CACGTG 1 cut(s) 1826
Ppu21I YACGTR 1 cut(s) 1826
PscI ACATGT 1 cut(s) 2361
Psp1406I AACGTT 1 cut(s) 1426
Psp6I CCWGG 8 cut(s) 799, 838, 889, 976, 1099, 1318, 2573, 2696
PspCI CACGTG 1 cut(s) 1826
PspEI GGTNACC 1 cut(s) 1994
PspFI CCCAGC 3 cut(s) 66, 163, 2014
PspGI CCWGG 8 cut(s) 799, 838, 889, 976, 1099, 1318, 2573, 2696
PspN4I GGNNCC 9 cut(s) 90, 735, 882, 937, 1662, 1763, 2409, 2572, 2789
PspPI GGNCC 8 cut(s) 626, 722, 836, 881, 1736, 1762, 2788, 2818
PsrI GAACNNNNNNTAC 2 cut(s) 3450, 3482
PstI CTGCAG 4 cut(s) 948, 1525, 2817, 3085
PstNI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
PsuI RGATCY 9 cut(s) 25, 153, 1255, 1358, 1660, 1832, 2407, 2570, 3281
PsyI GACNNNGTC 1 cut(s) 1926
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1171
RsaI GTAC 6 cut(s) 937, 973, 2396, 3149, 3304, 3463
RsaNI GTAC 6 cut(s) 936, 972, 2395, 3148, 3303, 3462
RseI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
Rsr2I CGGWCCG 1 cut(s) 722
RsrII CGGWCCG 1 cut(s) 722
SalI GTCGAC 1 cut(s) 1061
SapI GCTCTTC 2 cut(s) 1387, 1813
SaqAI TTAA 5 cut(s) 786, 926, 2283, 2451, 3006
Sau96I GGNCC 8 cut(s) 626, 722, 836, 881, 1736, 1762, 2788, 2818
ScaI AGTACT 1 cut(s) 3304
SduI GDGCHC 3 cut(s) 621, 1825, 3137
SexAI ACCWGGT 3 cut(s) 799, 889, 976
SfaNI GCATC 2 cut(s) 1462, 3229
SfcI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 406
SinI GGWCC 5 cut(s) 722, 836, 881, 1762, 2788
SmaI CCCGGG 1 cut(s) 852
SmiMI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
SmlI CTYRAG 3 cut(s) 128, 1435, 3005
SmoI CTYRAG 3 cut(s) 128, 1435, 3005
SseBI AGGCCT 1 cut(s) 1616
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 3268
SspMI CTAG 6 cut(s) 158, 212, 255, 1851, 2412, 3111
StuI AGGCCT 1 cut(s) 1616
StyI CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
TaaI ACNGT 8 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3466
TaiI ACGT 9 cut(s) 42, 279, 1429, 1698, 1828, 1927, 2263, 2557, 3319
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 2 cut(s) 3302, 3461
TauI GCSGC 9 cut(s) 94, 97, 236, 249, 401, 404, 425, 468, 671
TfiI GAWTC 7 cut(s) 221, 239, 296, 369, 382, 1514, 2313
Tru1I TTAA 5 cut(s) 786, 926, 2283, 2451, 3006
Tru9I TTAA 5 cut(s) 786, 926, 2283, 2451, 3006
TscAI CASTG 5 cut(s) 505, 619, 1908, 3181, 3316
TseI GCWGC 8 cut(s) 658, 943, 1274, 1277, 1961, 2165, 2655, 3080
TspGWI ACGGA 6 cut(s) 323, 1202, 1557, 1920, 2382, 2496
TspMI CCCGGG 1 cut(s) 850
TspRI CASTG 5 cut(s) 505, 619, 1908, 3181, 3316
Tth111I GACNNNGTC 1 cut(s) 1926
Van91I CCANNNNNTGG 1 cut(s) 890
Vha464I CTTAAG 1 cut(s) 3005
VpaK11BI GGWCC 5 cut(s) 722, 836, 881, 1762, 2788
XagI CCTNNNNNAGG 2 cut(s) 894, 1601
XapI RAATTY 7 cut(s) 17, 322, 952, 1025, 1309, 1874, 3358
XceI RCATGY 2 cut(s) 1751, 2365
XmaI CCCGGG 1 cut(s) 850
XmiI GTMKAC 3 cut(s) 1062, 1921, 2338
XmnI GAANNNNTTC 2 cut(s) 2250, 2808
XspI CTAG 6 cut(s) 158, 212, 255, 1851, 2412, 3111
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 3304
Zsp2I ATGCAT 1 cut(s) 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.